Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83431.t1 | XP_003612502.1 | 94.1 | 170 | 10 | 0 | 1 | 170 | 98 | 267 | 1.60E-79 | 305.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83431.t1 | G7K428 | 94.1 | 170 | 10 | 0 | 1 | 170 | 98 | 267 | 1.2e-79 | 305.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050508 | MS.gene83431 | 0.818415 | 1.98E-52 | -1.69E-46 |
| MS.gene059710 | MS.gene83431 | 0.834612 | 2.71E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83431.t1 | MTR_5g025750 | 94.118 | 170 | 10 | 0 | 1 | 170 | 98 | 267 | 3.57e-103 | 297 |
| MS.gene83431.t1 | MTR_4g068770 | 49.724 | 181 | 75 | 4 | 1 | 170 | 87 | 262 | 7.73e-53 | 169 |
| MS.gene83431.t1 | MTR_4g082590 | 38.150 | 173 | 93 | 3 | 2 | 168 | 127 | 291 | 1.29e-38 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83431.t1 | AT4G19390 | 65.882 | 170 | 58 | 0 | 1 | 170 | 16 | 185 | 1.90e-79 | 234 |
| MS.gene83431.t1 | AT4G19390 | 65.882 | 170 | 58 | 0 | 1 | 170 | 104 | 273 | 1.69e-77 | 232 |
| MS.gene83431.t1 | AT5G13720 | 49.724 | 181 | 75 | 4 | 1 | 170 | 86 | 261 | 3.78e-52 | 167 |
Find 23 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATAGATCAAATCTATT+TGG | 0.202531 | 5.4:-16842652 | MS.gene83431:CDS |
| TGTCGGTTTCTTGCTATTCT+TGG | 0.241873 | 5.4:-16843768 | MS.gene83431:CDS |
| GGAACGGTGATGCTAGTCTT+TGG | 0.339539 | 5.4:-16842742 | MS.gene83431:CDS |
| CTTCTCAGGAACGACCAAAA+TGG | 0.370797 | 5.4:-16842503 | MS.gene83431:intron |
| ATTTGGCTTATTCACTTTGA+AGG | 0.417143 | 5.4:-16842635 | MS.gene83431:intron |
| ATAGTGATGCTGCTTCTGAT+TGG | 0.427966 | 5.4:-16842432 | MS.gene83431:CDS |
| TTTGTTTGACAGAAGTAAGA+AGG | 0.477435 | 5.4:-16842409 | MS.gene83431:CDS |
| TGTAGGTGATTTATAGATGT+CGG | 0.486045 | 5.4:-16843785 | None:intergenic |
| CACTATCACATGACCAACCT+TGG | 0.497716 | 5.4:+16842449 | None:intergenic |
| TTTGCTGTCCAAGCTGAATG+AGG | 0.509196 | 5.4:-16842316 | MS.gene83431:CDS |
| TTAATTAGCCTCATTCAGCT+TGG | 0.510184 | 5.4:+16842308 | None:intergenic |
| CAAATCCACAGGAGTTTGAA+TGG | 0.513144 | 5.4:+16842383 | None:intergenic |
| ATCAATTCTATGCTTCATCA+AGG | 0.516481 | 5.4:-16843724 | MS.gene83431:intron |
| GCAATTGAATTACTTTACTA+CGG | 0.524763 | 5.4:+16843089 | None:intergenic |
| AATAGATTTGATCTATCAGA+AGG | 0.529196 | 5.4:+16842655 | None:intergenic |
| GTGATGCTAGTCTTTGGAAT+GGG | 0.541127 | 5.4:-16842736 | MS.gene83431:CDS |
| CAGAAGCATAGCAAATCCAC+AGG | 0.569222 | 5.4:+16842372 | None:intergenic |
| GGTGATGCTAGTCTTTGGAA+TGG | 0.578084 | 5.4:-16842737 | MS.gene83431:CDS |
| AAAAGACTCTGCAACAAATG+TGG | 0.581983 | 5.4:+16843129 | None:intergenic |
| CAATTGCTGATAGAAGCTCT+TGG | 0.584191 | 5.4:-16843073 | MS.gene83431:intron |
| AATGAACTGAAAACCAAGGT+TGG | 0.611400 | 5.4:-16842462 | MS.gene83431:CDS |
| GGCTACCATTCAAACTCCTG+TGG | 0.627628 | 5.4:-16842388 | MS.gene83431:CDS |
| AGTGAATGAACTGAAAACCA+AGG | 0.699304 | 5.4:-16842466 | MS.gene83431:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTGTTTTTTTTTTTTTTT+TGG | + | chr5.4:16842435-16842454 | None:intergenic | 10.0% |
| !!! | AACAAATTATACAAACTTTT+AGG | + | chr5.4:16843063-16843082 | None:intergenic | 15.0% |
| !! | AATTCTTCATATAATCAACT+TGG | - | chr5.4:16843114-16843133 | MS.gene83431:CDS | 20.0% |
| !!! | AGATTTGCTTAACTAAATTT+GGG | + | chr5.4:16842712-16842731 | None:intergenic | 20.0% |
| !!! | GTTTTTATATTCAACCATTT+TGG | + | chr5.4:16843602-16843621 | None:intergenic | 20.0% |
| ! | AATAGATTTGATCTATCAGA+AGG | + | chr5.4:16843436-16843455 | None:intergenic | 25.0% |
| ! | ATTATTCAATTTGACATGCA+GGG | - | chr5.4:16842934-16842953 | MS.gene83431:intron | 25.0% |
| ! | ATTATTCACTTTCTCAAACT+GGG | - | chr5.4:16842833-16842852 | MS.gene83431:intron | 25.0% |
| ! | GCAATTGAATTACTTTACTA+CGG | + | chr5.4:16843002-16843021 | None:intergenic | 25.0% |
| ! | TATGATAGCATATTAGAATC+TGG | + | chr5.4:16842574-16842593 | None:intergenic | 25.0% |
| ! | TCTGATAGATCAAATCTATT+TGG | - | chr5.4:16843436-16843455 | MS.gene83431:intron | 25.0% |
| ! | TTGAGAAAGTGAATAATCTT+CGG | + | chr5.4:16842830-16842849 | None:intergenic | 25.0% |
| !! | AATAGTATGCTTTTGTGATT+TGG | - | chr5.4:16842483-16842502 | MS.gene83431:CDS | 25.0% |
| !! | GAGATTTGCTTAACTAAATT+TGG | + | chr5.4:16842713-16842732 | None:intergenic | 25.0% |
| !! | TCACAAAAGCATACTATTAA+AGG | + | chr5.4:16842481-16842500 | None:intergenic | 25.0% |
| !!! | GTTAGACCTAAATTTTAACA+CGG | + | chr5.4:16843166-16843185 | None:intergenic | 25.0% |
| ACTGAATAGTAACTACAAGT+GGG | - | chr5.4:16842506-16842525 | MS.gene83431:intron | 30.0% | |
| ATCAATTCTATGCTTCATCA+AGG | - | chr5.4:16842364-16842383 | MS.gene83431:CDS | 30.0% | |
| CATTATTCAATTTGACATGC+AGG | - | chr5.4:16842933-16842952 | MS.gene83431:intron | 30.0% | |
| CTGATACCGTGTTAAAATTT+AGG | - | chr5.4:16843157-16843176 | MS.gene83431:intron | 30.0% | |
| GATTATTCACTTTCTCAAAC+TGG | - | chr5.4:16842832-16842851 | MS.gene83431:intron | 30.0% | |
| GTTAGGCTTAAATTTGAAGA+TGG | - | chr5.4:16843218-16843237 | MS.gene83431:intron | 30.0% | |
| TGTGATTCTCTTCTTTCTAA+CGG | - | chr5.4:16843289-16843308 | MS.gene83431:intron | 30.0% | |
| TTTGTTTGACAGAAGTAAGA+AGG | - | chr5.4:16843679-16843698 | MS.gene83431:intron | 30.0% | |
| ! | ACTCTAAAAGCTATCTCATA+TGG | + | chr5.4:16842674-16842693 | None:intergenic | 30.0% |
| ! | ATTTGGCTTATTCACTTTGA+AGG | - | chr5.4:16843453-16843472 | MS.gene83431:intron | 30.0% |
| ! | CATGTAGATCTCTATCTTTT+AGG | - | chr5.4:16843325-16843344 | MS.gene83431:intron | 30.0% |
| !! | AAATCCTACTGACTTTGATA+AGG | + | chr5.4:16843510-16843529 | None:intergenic | 30.0% |
| !! | AGATCTCTATCTTTTAGGAA+CGG | - | chr5.4:16843330-16843349 | MS.gene83431:intron | 30.0% |
| !! | TTGCTATTCTTGGAGTTTTT+GGG | - | chr5.4:16842330-16842349 | MS.gene83431:CDS | 30.0% |
| !!! | ATTTGTTGCAGAGTCTTTTT+TGG | - | chr5.4:16842963-16842982 | MS.gene83431:intron | 30.0% |
| !!! | TTTGTTGCAGAGTCTTTTTT+GGG | - | chr5.4:16842964-16842983 | MS.gene83431:intron | 30.0% |
| AAAAGACTCTGCAACAAATG+TGG | + | chr5.4:16842962-16842981 | None:intergenic | 35.0% | |
| AAAAGCTATCTCATATGGTG+AGG | + | chr5.4:16842669-16842688 | None:intergenic | 35.0% | |
| AATGAACTGAAAACCAAGGT+TGG | - | chr5.4:16843626-16843645 | MS.gene83431:intron | 35.0% | |
| ACTTTCCTTAATTCGTACCA+AGG | + | chr5.4:16842767-16842786 | None:intergenic | 35.0% | |
| AGTGAATGAACTGAAAACCA+AGG | - | chr5.4:16843622-16843641 | MS.gene83431:intron | 35.0% | |
| CTAACTCATCGTTACAAAAC+TGG | + | chr5.4:16843203-16843222 | None:intergenic | 35.0% | |
| CTTTCCTTAATTCGTACCAA+GGG | + | chr5.4:16842766-16842785 | None:intergenic | 35.0% | |
| GACAACACTAACACAAAAGT+AGG | + | chr5.4:16843248-16843267 | None:intergenic | 35.0% | |
| GACTGAATAGTAACTACAAG+TGG | - | chr5.4:16842505-16842524 | MS.gene83431:intron | 35.0% | |
| TAAAAGCTAGCTCATCATTG+AGG | + | chr5.4:16842537-16842556 | None:intergenic | 35.0% | |
| TCAAAGTCAGTAGGATTTAG+TGG | - | chr5.4:16843512-16843531 | MS.gene83431:intron | 35.0% | |
| TCAACCTTATCAAAGTCAGT+AGG | - | chr5.4:16843503-16843522 | MS.gene83431:intron | 35.0% | |
| TGACTTTGATAAGGTTGACT+AGG | + | chr5.4:16843501-16843520 | None:intergenic | 35.0% | |
| TTAACCCTTGGTACGAATTA+AGG | - | chr5.4:16842759-16842778 | MS.gene83431:CDS | 35.0% | |
| ! | AGTGTTGTCATTGTTCTAAG+AGG | - | chr5.4:16843259-16843278 | MS.gene83431:intron | 35.0% |
| ! | TTAATTAGCCTCATTCAGCT+TGG | + | chr5.4:16843783-16843802 | None:intergenic | 35.0% |
| !! | CAGTTTTGTAACGATGAGTT+AGG | - | chr5.4:16843201-16843220 | MS.gene83431:intron | 35.0% |
| !!! | AGAGTCTTTTTTGGGATACT+TGG | - | chr5.4:16842972-16842991 | MS.gene83431:intron | 35.0% |
| !!! | CTTGCTATTCTTGGAGTTTT+TGG | - | chr5.4:16842329-16842348 | MS.gene83431:CDS | 35.0% |
| !!! | CTTTTAGAGTTGAGTTAAGC+TGG | - | chr5.4:16842684-16842703 | MS.gene83431:CDS | 35.0% |
| CAAATCCACAGGAGTTTGAA+TGG | + | chr5.4:16843708-16843727 | None:intergenic | 40.0% | |
| CAATTGCTGATAGAAGCTCT+TGG | - | chr5.4:16843015-16843034 | MS.gene83431:intron | 40.0% | |
| CCAATTCGTAAGTACATGTC+AGG | - | chr5.4:16842735-16842754 | MS.gene83431:CDS | 40.0% | |
| CCTGACATGTACTTACGAAT+TGG | + | chr5.4:16842738-16842757 | None:intergenic | 40.0% | |
| TCTCATATGGTGAGGTTTGT+AGG | + | chr5.4:16842661-16842680 | None:intergenic | 40.0% | |
| TTCCAATGTTTCTCCTTCTC+AGG | - | chr5.4:16843571-16843590 | MS.gene83431:intron | 40.0% | |
| TTCCTGAGAAGGAGAAACAT+TGG | + | chr5.4:16843576-16843595 | None:intergenic | 40.0% | |
| ! | ATAGTGATGCTGCTTCTGAT+TGG | - | chr5.4:16843656-16843675 | MS.gene83431:intron | 40.0% |
| !! | GTGATGCTAGTCTTTGGAAT+GGG | - | chr5.4:16843352-16843371 | MS.gene83431:intron | 40.0% |
| !! | TGTCGGTTTCTTGCTATTCT+TGG | - | chr5.4:16842320-16842339 | MS.gene83431:CDS | 40.0% |
| !!! | GGAGTTTTTGGGTCTTTGAT+TGG | - | chr5.4:16842341-16842360 | MS.gene83431:CDS | 40.0% |
| AGCATCAAAATGCAGCAGAG+AGG | + | chr5.4:16843549-16843568 | None:intergenic | 45.0% | |
| CACAGGACTGCACATTTACA+GGG | + | chr5.4:16842614-16842633 | None:intergenic | 45.0% | |
| CACTATCACATGACCAACCT+TGG | + | chr5.4:16843642-16843661 | None:intergenic | 45.0% | |
| CTGCACATTTACAGGGATCT+AGG | + | chr5.4:16842607-16842626 | None:intergenic | 45.0% | |
| CTTCTCAGGAACGACCAAAA+TGG | - | chr5.4:16843585-16843604 | MS.gene83431:intron | 45.0% | |
| GGTTTGTAGGACTAGACATC+CGG | + | chr5.4:16842648-16842667 | None:intergenic | 45.0% | |
| TACATGTCAGGCTTAACCCT+TGG | - | chr5.4:16842747-16842766 | MS.gene83431:CDS | 45.0% | |
| TCACAGGACTGCACATTTAC+AGG | + | chr5.4:16842615-16842634 | None:intergenic | 45.0% | |
| ! | CAGAAGCATAGCAAATCCAC+AGG | + | chr5.4:16843719-16843738 | None:intergenic | 45.0% |
| ! | GGTATCAGAGCCTATCGATT+CGG | + | chr5.4:16843145-16843164 | None:intergenic | 45.0% |
| ! | TTTGCTGTCCAAGCTGAATG+AGG | - | chr5.4:16843772-16843791 | MS.gene83431:CDS | 45.0% |
| !! | CATTTTGGTCGTTCCTGAGA+AGG | + | chr5.4:16843587-16843606 | None:intergenic | 45.0% |
| !! | GGTGATGCTAGTCTTTGGAA+TGG | - | chr5.4:16843351-16843370 | MS.gene83431:intron | 45.0% |
| !! | GTATCAGAGCCTATCGATTC+GGG | + | chr5.4:16843144-16843163 | None:intergenic | 45.0% |
| ATCCGGAGCGTGAAATTCAC+AGG | + | chr5.4:16842631-16842650 | None:intergenic | 50.0% | |
| CTTGGATAGCCCGAATCGAT+AGG | - | chr5.4:16843132-16843151 | MS.gene83431:CDS | 50.0% | |
| GGCTACCATTCAAACTCCTG+TGG | - | chr5.4:16843700-16843719 | MS.gene83431:intron | 50.0% | |
| GTCCTGTGAATTTCACGCTC+CGG | - | chr5.4:16842626-16842645 | MS.gene83431:intron | 50.0% | |
| !! | GGAACGGTGATGCTAGTCTT+TGG | - | chr5.4:16843346-16843365 | MS.gene83431:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 16842308 | 16843802 | 16842308 | ID=MS.gene83431 |
| chr5.4 | mRNA | 16842308 | 16843802 | 16842308 | ID=MS.gene83431.t1;Parent=MS.gene83431 |
| chr5.4 | exon | 16843725 | 16843802 | 16843725 | ID=MS.gene83431.t1.exon1;Parent=MS.gene83431.t1 |
| chr5.4 | CDS | 16843725 | 16843802 | 16843725 | ID=cds.MS.gene83431.t1;Parent=MS.gene83431.t1 |
| chr5.4 | exon | 16843074 | 16843155 | 16843074 | ID=MS.gene83431.t1.exon2;Parent=MS.gene83431.t1 |
| chr5.4 | CDS | 16843074 | 16843155 | 16843074 | ID=cds.MS.gene83431.t1;Parent=MS.gene83431.t1 |
| chr5.4 | exon | 16842636 | 16842778 | 16842636 | ID=MS.gene83431.t1.exon3;Parent=MS.gene83431.t1 |
| chr5.4 | CDS | 16842636 | 16842778 | 16842636 | ID=cds.MS.gene83431.t1;Parent=MS.gene83431.t1 |
| chr5.4 | exon | 16842308 | 16842517 | 16842308 | ID=MS.gene83431.t1.exon4;Parent=MS.gene83431.t1 |
| chr5.4 | CDS | 16842308 | 16842517 | 16842308 | ID=cds.MS.gene83431.t1;Parent=MS.gene83431.t1 |
| Gene Sequence |
| Protein sequence |
