Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83854.t1 | XP_024629673.1 | 94.3 | 53 | 3 | 0 | 1 | 53 | 65 | 117 | 1.40E-19 | 104.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83854.t1 | A0A072TRQ9 | 98.0 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 1.3e-19 | 104.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059894 | MS.gene83854 | 0.805451 | 1.33E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene83854 | 0.800817 | 1.21E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83854.t1 | MTR_8g069835 | 98.000 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 1.09e-31 | 103 |
| MS.gene83854.t1 | MTR_7g100335 | 52.000 | 50 | 24 | 0 | 1 | 50 | 24 | 73 | 1.04e-14 | 62.0 |
| MS.gene83854.t1 | MTR_7g022840 | 56.410 | 39 | 17 | 0 | 1 | 39 | 31 | 69 | 2.94e-13 | 58.9 |
| MS.gene83854.t1 | MTR_0001s0190 | 53.846 | 39 | 18 | 0 | 1 | 39 | 26 | 64 | 2.18e-11 | 53.9 |
| MS.gene83854.t1 | MTR_1g115490 | 51.282 | 39 | 19 | 0 | 1 | 39 | 26 | 64 | 5.32e-11 | 53.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83854.t1 | AT1G10657 | 57.143 | 49 | 20 | 1 | 1 | 49 | 39 | 86 | 8.43e-16 | 65.5 |
| MS.gene83854.t1 | AT1G10657 | 57.143 | 49 | 20 | 1 | 1 | 49 | 39 | 86 | 9.27e-16 | 65.1 |
| MS.gene83854.t1 | AT3G55240 | 58.974 | 39 | 16 | 0 | 1 | 39 | 25 | 63 | 2.88e-14 | 61.2 |
| MS.gene83854.t1 | AT3G28990 | 43.636 | 55 | 31 | 0 | 1 | 55 | 25 | 79 | 1.22e-13 | 59.3 |
| MS.gene83854.t1 | AT5G02580 | 56.410 | 39 | 17 | 0 | 1 | 39 | 27 | 65 | 6.84e-12 | 55.1 |
| MS.gene83854.t1 | AT5G02580 | 56.410 | 39 | 17 | 0 | 1 | 39 | 20 | 58 | 1.19e-11 | 54.3 |
Find 10 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCCACTTGTTACTCTTAC+AGG | 0.198423 | 8.3:+32917743 | MS.gene83854:CDS |
| CTTCTAGTGCCTTTATGCAT+TGG | 0.385653 | 8.3:-32917706 | None:intergenic |
| TTTACGTACGTGAACGAGCT+TGG | 0.432955 | 8.3:-32917925 | None:intergenic |
| GAGTCAAGACCAATGCATAA+AGG | 0.483045 | 8.3:+32917697 | MS.gene83854:CDS |
| CGTACGTGAACGAGCTTGGA+GGG | 0.493662 | 8.3:-32917921 | None:intergenic |
| ACGTACGTGAACGAGCTTGG+AGG | 0.547000 | 8.3:-32917922 | None:intergenic |
| AGTGTGGAGGGAGTTGCAAA+AGG | 0.569445 | 8.3:+32917858 | MS.gene83854:intron |
| AAGGCACTAGAAGAACATGC+AGG | 0.583674 | 8.3:+32917716 | MS.gene83854:CDS |
| AGGCACTAGAAGAACATGCA+GGG | 0.629832 | 8.3:+32917717 | MS.gene83854:CDS |
| AGACCTGTAAGAGTAACAAG+TGG | 0.670192 | 8.3:-32917746 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAACTAAATTATATGC+AGG | + | chr8.3:32918008-32918027 | MS.gene83854:intron | 15.0% |
| !!! | AAATTCCATATATATTTTCA+AGG | + | chr8.3:32917954-32917973 | MS.gene83854:intron | 15.0% |
| ! | CTGATCCTTGAAAATATATA+TGG | - | chr8.3:32917962-32917981 | None:intergenic | 25.0% |
| ! | TGAAACTTATGTTACACTTT+GGG | + | chr8.3:32917793-32917812 | MS.gene83854:intron | 25.0% |
| ! | TTGAAACTTATGTTACACTT+TGG | + | chr8.3:32917792-32917811 | MS.gene83854:intron | 25.0% |
| TATGCTTTGCACATGATTAA+AGG | - | chr8.3:32917988-32918007 | None:intergenic | 30.0% | |
| ! | TACACTTTGGGAAACATATA+TGG | + | chr8.3:32917805-32917824 | MS.gene83854:intron | 30.0% |
| ! | TGTATATTCATTTTGCAGTG+TGG | + | chr8.3:32917842-32917861 | MS.gene83854:intron | 30.0% |
| ! | ATATTCATTTTGCAGTGTGG+AGG | + | chr8.3:32917845-32917864 | MS.gene83854:intron | 35.0% |
| ! | TATTCATTTTGCAGTGTGGA+GGG | + | chr8.3:32917846-32917865 | MS.gene83854:intron | 35.0% |
| AGACCTGTAAGAGTAACAAG+TGG | - | chr8.3:32917749-32917768 | None:intergenic | 40.0% | |
| CTTCTAGTGCCTTTATGCAT+TGG | - | chr8.3:32917709-32917728 | None:intergenic | 40.0% | |
| GAGTCAAGACCAATGCATAA+AGG | + | chr8.3:32917697-32917716 | MS.gene83854:CDS | 40.0% | |
| AGGCACTAGAAGAACATGCA+GGG | + | chr8.3:32917717-32917736 | MS.gene83854:CDS | 45.0% | |
| TTTACGTACGTGAACGAGCT+TGG | - | chr8.3:32917928-32917947 | None:intergenic | 45.0% | |
| ! | AAGGCACTAGAAGAACATGC+AGG | + | chr8.3:32917716-32917735 | MS.gene83854:CDS | 45.0% |
| ! | CAGCCACTTGTTACTCTTAC+AGG | + | chr8.3:32917743-32917762 | MS.gene83854:CDS | 45.0% |
| AGTGTGGAGGGAGTTGCAAA+AGG | + | chr8.3:32917858-32917877 | MS.gene83854:intron | 50.0% | |
| ACGTACGTGAACGAGCTTGG+AGG | - | chr8.3:32917925-32917944 | None:intergenic | 55.0% | |
| CGTACGTGAACGAGCTTGGA+GGG | - | chr8.3:32917924-32917943 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 32917695 | 32918054 | 32917695 | ID=MS.gene83854 |
| chr8.3 | mRNA | 32917695 | 32918054 | 32917695 | ID=MS.gene83854.t1;Parent=MS.gene83854 |
| chr8.3 | exon | 32917695 | 32917764 | 32917695 | ID=MS.gene83854.t1.exon1;Parent=MS.gene83854.t1 |
| chr8.3 | CDS | 32917695 | 32917764 | 32917695 | ID=cds.MS.gene83854.t1;Parent=MS.gene83854.t1 |
| chr8.3 | exon | 32917860 | 32917938 | 32917860 | ID=MS.gene83854.t1.exon2;Parent=MS.gene83854.t1 |
| chr8.3 | CDS | 32917860 | 32917938 | 32917860 | ID=cds.MS.gene83854.t1;Parent=MS.gene83854.t1 |
| chr8.3 | exon | 32918030 | 32918054 | 32918030 | ID=MS.gene83854.t1.exon3;Parent=MS.gene83854.t1 |
| chr8.3 | CDS | 32918030 | 32918054 | 32918030 | ID=cds.MS.gene83854.t1;Parent=MS.gene83854.t1 |
| Gene Sequence |
| Protein sequence |
