AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83911

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83911.t1 MTR_7g098750 88.182 110 13 0 113 222 675 784 4.10e-57 196
MS.gene83911.t1 MTR_7g098750 88.182 110 13 0 113 222 945 1054 5.05e-57 196
MS.gene83911.t1 MTR_7g098750 88.182 110 13 0 113 222 945 1054 5.85e-57 196
MS.gene83911.t1 MTR_7g098300 87.273 110 14 0 113 222 941 1050 7.43e-57 196
MS.gene83911.t1 MTR_7g098740 74.306 144 29 2 113 255 903 1039 7.69e-57 196
MS.gene83911.t1 MTR_7g098760 85.455 110 16 0 113 222 942 1051 3.48e-55 191
MS.gene83911.t1 MTR_7g098370 86.364 110 15 0 113 222 903 1012 3.53e-55 191
MS.gene83911.t1 MTR_7g098320 81.818 110 20 0 113 222 937 1046 4.56e-53 185
MS.gene83911.t1 MTR_7g098320 81.818 110 20 0 113 222 937 1046 5.17e-53 184
MS.gene83911.t1 MTR_4g011630 66.667 144 40 2 113 255 928 1064 5.07e-52 182
MS.gene83911.t1 MTR_7g104110 80.000 110 22 0 113 222 937 1046 1.33e-51 181
MS.gene83911.t1 MTR_7g098800 64.430 149 35 2 113 255 865 1001 3.14e-51 179
MS.gene83911.t1 MTR_7g104130 79.091 110 23 0 113 222 945 1054 4.11e-51 179
MS.gene83911.t1 MTR_7g104130 79.091 110 23 0 113 222 945 1054 4.11e-51 179
MS.gene83911.t1 MTR_2g102670 65.972 144 41 2 113 255 900 1036 4.91e-51 179
MS.gene83911.t1 MTR_2g102660 65.972 144 41 2 113 255 917 1053 4.92e-51 179
MS.gene83911.t1 MTR_4g011620 66.667 144 40 2 113 255 918 1054 4.97e-51 179
MS.gene83911.t1 MTR_2g102640 65.972 144 41 2 113 255 920 1056 6.32e-51 179
MS.gene83911.t1 MTR_3g107870 78.899 109 23 0 113 221 952 1060 1.49e-50 177
MS.gene83911.t1 MTR_4g011640 71.311 122 34 1 102 222 913 1034 2.39e-50 177
MS.gene83911.t1 MTR_7g098780 65.000 140 43 1 113 246 907 1046 2.84e-50 177
MS.gene83911.t1 MTR_7g104150 77.273 110 25 0 113 222 945 1054 7.98e-50 176
MS.gene83911.t1 MTR_1g011640 78.182 110 24 0 113 222 916 1025 5.84e-49 173
MS.gene83911.t1 MTR_1g011650 77.273 110 25 0 113 222 939 1048 1.93e-48 171
MS.gene83911.t1 MTR_1g050525 73.636 110 29 0 113 222 956 1065 3.62e-48 171
MS.gene83911.t1 MTR_1g492950 73.394 109 29 0 113 221 956 1064 7.24e-48 170
MS.gene83911.t1 MTR_7g104100 74.545 110 28 0 113 222 943 1052 1.82e-47 169
MS.gene83911.t1 MTR_4g094235 94.872 78 4 0 1 78 1 78 2.20e-47 159
MS.gene83911.t1 MTR_4g094235 94.872 78 4 0 1 78 1 78 2.26e-47 159
MS.gene83911.t1 MTR_4g113070 72.642 106 29 0 113 218 906 1011 8.87e-46 164
MS.gene83911.t1 MTR_5g070450 91.026 78 7 0 1 78 1 78 1.44e-45 154
MS.gene83911.t1 MTR_7g098890 74.528 106 26 1 113 218 846 950 3.28e-44 159
MS.gene83911.t1 MTR_8g014360 69.725 109 33 0 113 221 967 1075 6.39e-44 158
MS.gene83911.t1 MTR_8g014360 69.725 109 33 0 113 221 967 1075 8.61e-44 158
MS.gene83911.t1 MTR_2g101090 68.807 109 34 0 113 221 933 1041 1.10e-42 155
MS.gene83911.t1 MTR_5g070320 67.890 109 35 0 113 221 920 1028 1.17e-39 146
MS.gene83911.t1 MTR_5g070320 67.273 110 36 0 113 222 633 742 1.53e-39 146
MS.gene83911.t1 MTR_5g070320 67.890 109 35 0 113 221 729 837 1.91e-39 145
MS.gene83911.t1 MTR_3g463680 66.055 109 37 0 113 221 905 1013 2.96e-38 142
MS.gene83911.t1 MTR_4g123850 65.263 95 33 0 113 207 920 1014 8.29e-35 132
MS.gene83911.t1 MTR_7g407080 61.468 109 13 1 113 221 170 249 1.48e-32 125
MS.gene83911.t1 MTR_8g103450 46.565 131 45 1 113 243 310 415 2.86e-28 111
MS.gene83911.t1 MTR_7g060570 48.246 114 41 2 113 217 990 1094 9.14e-22 94.7
MS.gene83911.t1 MTR_1g094660 33.654 104 69 0 113 216 579 682 7.06e-16 77.4
MS.gene83911.t1 MTR_1g094660 33.654 104 69 0 113 216 579 682 8.21e-16 77.0
MS.gene83911.t1 MTR_1g054935 33.562 146 85 2 113 255 232 368 9.63e-16 77.0
MS.gene83911.t1 MTR_1g054935 33.562 146 85 2 113 255 70 206 1.82e-15 75.9
MS.gene83911.t1 MTR_8g059150 33.333 108 72 0 113 220 126 233 2.72e-15 75.5
MS.gene83911.t1 MTR_5g025470 31.132 106 73 0 113 218 125 230 5.71e-15 74.3
MS.gene83911.t1 MTR_1g054960 34.247 146 84 2 113 255 85 221 6.36e-15 74.3
MS.gene83911.t1 MTR_2g079980 33.945 109 68 1 113 221 91 195 1.17e-14 73.6
MS.gene83911.t1 MTR_7g106880 32.877 146 86 3 113 255 221 357 1.57e-14 73.2
MS.gene83911.t1 MTR_7g106880 32.192 146 87 2 113 255 70 206 2.56e-14 72.4
MS.gene83911.t1 MTR_1g063920 37.895 95 59 0 113 207 555 649 3.75e-14 72.4
MS.gene83911.t1 MTR_5g030910 32.407 108 72 1 113 220 113 219 4.78e-14 72.0
MS.gene83911.t1 MTR_1g108340 35.616 146 82 4 113 255 156 292 5.34e-14 71.6
MS.gene83911.t1 MTR_1g108340 35.616 146 82 4 113 255 156 292 5.70e-14 71.6
MS.gene83911.t1 MTR_4g094050 32.759 116 78 0 103 218 124 239 8.45e-14 71.2
MS.gene83911.t1 MTR_8g107450 27.206 136 92 2 113 244 131 263 1.07e-13 70.9
MS.gene83911.t1 MTR_4g094060 31.897 116 79 0 103 218 124 239 2.57e-13 69.7
MS.gene83911.t1 MTR_1g093990 34.286 105 67 1 113 215 150 254 2.98e-13 69.3
MS.gene83911.t1 MTR_4g076970 32.051 156 94 3 103 255 102 248 3.31e-13 69.3
MS.gene83911.t1 MTR_4g054020 31.897 116 79 0 103 218 21 136 4.43e-13 68.9
MS.gene83911.t1 MTR_1g096580 33.333 99 66 0 113 211 151 249 6.19e-13 68.6
MS.gene83911.t1 MTR_7g100120 25.926 135 95 2 113 244 189 321 6.91e-13 68.6
MS.gene83911.t1 MTR_4g058000 36.264 91 58 0 113 203 569 659 1.49e-12 67.4
MS.gene83911.t1 MTR_7g101780 34.737 95 62 0 113 207 576 670 1.58e-12 67.4
MS.gene83911.t1 MTR_4g094010 31.897 116 79 0 103 218 101 216 1.63e-12 67.4
MS.gene83911.t1 MTR_4g128980 53.125 64 29 1 176 238 27 90 2.41e-12 62.8
MS.gene83911.t1 MTR_1g115790 24.444 135 97 2 113 244 184 316 1.06e-11 64.7
MS.gene83911.t1 MTR_4g076900 29.944 177 110 5 84 255 59 226 1.21e-11 64.7
MS.gene83911.t1 MTR_4g094090 33.333 111 74 0 113 223 92 202 1.94e-11 63.9
MS.gene83911.t1 MTR_5g096390 30.303 99 69 0 113 211 169 267 2.14e-11 63.9
MS.gene83911.t1 MTR_4g076940 29.412 170 107 4 90 255 90 250 2.74e-11 63.5
MS.gene83911.t1 MTR_8g015780 51.562 64 28 1 158 221 1 61 3.43e-11 60.1
MS.gene83911.t1 MTR_4g076940 29.412 170 107 4 90 255 130 290 3.67e-11 63.2
MS.gene83911.t1 MTR_2g078080 34.862 109 67 2 113 219 112 218 3.91e-11 63.2
MS.gene83911.t1 MTR_4g076940 30.128 156 97 3 103 255 11 157 4.94e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83911.t1 AT2G36380 79.817 109 22 0 113 221 941 1049 8.61e-52 181
MS.gene83911.t1 AT1G66950 78.899 109 23 0 113 221 942 1050 3.72e-51 179
MS.gene83911.t1 AT1G15520 65.972 144 41 2 113 255 914 1050 1.39e-49 175
MS.gene83911.t1 AT1G15520 65.972 144 41 2 113 255 914 1050 1.53e-49 175
MS.gene83911.t1 AT3G30842 63.889 144 44 2 113 255 910 1046 2.82e-47 168
MS.gene83911.t1 AT2G26910 61.594 138 50 2 84 218 875 1012 9.78e-44 158
MS.gene83911.t1 AT1G15210 70.642 109 32 0 113 221 929 1037 1.28e-43 157
MS.gene83911.t1 AT1G59870 57.857 140 53 1 113 246 956 1095 1.72e-43 157
MS.gene83911.t1 AT2G37280 57.143 140 54 1 113 246 744 883 4.34e-43 156
MS.gene83911.t1 AT2G37280 57.143 140 54 1 113 246 902 1041 4.88e-43 156
MS.gene83911.t1 AT2G37280 57.143 140 54 1 113 246 902 1041 5.36e-43 156
MS.gene83911.t1 AT2G37280 57.143 140 54 1 113 246 931 1070 5.92e-43 155
MS.gene83911.t1 AT5G64200 84.615 78 12 0 1 78 1 78 6.27e-43 148
MS.gene83911.t1 AT5G64200 84.615 78 12 0 1 78 1 78 6.27e-43 148
MS.gene83911.t1 AT3G16340 59.286 140 54 2 85 221 872 1011 1.50e-42 154
MS.gene83911.t1 AT3G16340 59.286 140 54 2 85 221 867 1006 1.54e-42 154
MS.gene83911.t1 AT3G53480 67.925 106 34 0 113 218 939 1044 7.64e-42 152
MS.gene83911.t1 AT4G15236 66.038 106 36 0 113 218 879 984 4.54e-41 150
MS.gene83911.t1 AT4G15230 66.981 106 35 0 113 218 889 994 6.75e-41 150
MS.gene83911.t1 AT4G15230 66.981 106 35 0 113 218 810 915 6.81e-41 150
MS.gene83911.t1 AT4G15230 66.981 106 35 0 113 218 889 994 7.14e-41 150
MS.gene83911.t1 AT4G15230 66.981 106 35 0 113 218 889 994 7.14e-41 150
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 879 984 7.63e-41 150
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 876 981 8.08e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 876 981 8.08e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 876 981 8.08e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 876 981 8.08e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 883 988 8.65e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 883 988 8.65e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 883 988 8.65e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 883 988 8.65e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 883 988 8.65e-41 149
MS.gene83911.t1 AT4G15215 66.038 106 36 0 113 218 886 991 8.90e-41 149
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 881 986 1.85e-40 149
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 872 977 1.94e-40 149
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 819 924 2.10e-40 148
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 819 924 2.10e-40 148
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 881 986 2.19e-40 148
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 863 968 2.21e-40 148
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 738 843 2.85e-40 148
MS.gene83911.t1 AT4G15233 65.094 106 37 0 113 218 738 843 2.85e-40 148
MS.gene83911.t1 AT2G29940 69.725 109 33 0 113 221 915 1023 5.76e-40 147
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.26e-15 76.3
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.26e-15 76.3
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.32e-15 76.6
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.34e-15 76.3
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.34e-15 76.3
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.34e-15 76.6
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.34e-15 76.6
MS.gene83911.t1 AT2G01320 36.986 146 80 5 113 255 153 289 1.34e-15 76.6
MS.gene83911.t1 AT5G06530 32.877 146 86 3 113 255 240 376 6.87e-15 74.3
MS.gene83911.t1 AT5G06530 32.877 146 86 3 113 255 240 376 6.87e-15 74.3
MS.gene83911.t1 AT5G06530 32.877 146 86 3 113 255 240 376 6.88e-15 74.3
MS.gene83911.t1 AT1G53390 30.631 111 77 0 113 223 585 695 1.02e-14 73.9
MS.gene83911.t1 AT5G06530 32.877 146 86 3 113 255 45 181 1.05e-14 73.6
MS.gene83911.t1 AT1G31770 33.333 99 66 0 113 211 141 239 1.11e-14 73.6
MS.gene83911.t1 AT1G71960 37.374 99 62 0 113 211 144 242 6.86e-14 71.2
MS.gene83911.t1 AT3G25620 33.981 103 68 0 113 215 159 261 8.03e-14 70.9
MS.gene83911.t1 AT2G37010 33.654 104 69 0 113 216 562 665 1.05e-13 70.9
MS.gene83911.t1 AT2G37010 33.654 104 69 0 113 216 562 665 1.05e-13 70.9
MS.gene83911.t1 AT3G25620 35.000 100 65 0 113 212 159 258 1.28e-13 70.5
MS.gene83911.t1 AT1G51500 31.613 155 97 2 103 255 95 242 2.43e-13 69.7
MS.gene83911.t1 AT1G51460 35.088 114 74 0 103 216 81 194 3.06e-13 69.3
MS.gene83911.t1 AT3G55090 25.185 135 96 2 113 244 169 301 4.47e-13 68.9
MS.gene83911.t1 AT3G21090 30.128 156 97 3 103 255 96 242 4.55e-13 68.9
MS.gene83911.t1 AT3G55130 25.185 135 96 2 113 244 162 294 6.71e-13 68.6
MS.gene83911.t1 AT5G13580 26.087 115 85 0 102 216 159 273 1.30e-12 67.8
MS.gene83911.t1 AT3G55100 28.846 104 74 0 113 216 113 216 1.32e-12 67.4
MS.gene83911.t1 AT3G55100 28.846 104 74 0 113 216 113 216 1.32e-12 67.4
MS.gene83911.t1 AT2G37360 25.926 135 95 2 113 244 195 327 1.35e-12 67.8
MS.gene83911.t1 AT4G27420 31.481 108 74 0 113 220 129 236 1.48e-12 67.4
MS.gene83911.t1 AT2G39350 24.444 135 97 2 113 244 171 303 1.61e-12 67.4
MS.gene83911.t1 AT2G13610 30.709 127 85 2 89 215 99 222 1.86e-12 67.0
MS.gene83911.t1 AT3G55110 23.737 198 144 4 50 244 93 286 1.96e-12 67.0
MS.gene83911.t1 AT3G13220 30.137 146 90 2 113 255 166 302 2.82e-12 66.6
MS.gene83911.t1 AT3G53510 25.926 135 95 2 113 244 188 320 3.20e-11 63.5
MS.gene83911.t1 AT1G53270 33.333 114 68 2 113 223 112 220 4.82e-11 62.8

Find 70 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTAAAATACTAACCTTTC+TGG 0.119600 6.1:+8859816 None:intergenic
AACAAAATTAAAGGAGAAAA+AGG 0.164946 6.1:+8859421 None:intergenic
CATTCTATGGTCGAGTTCTT+TGG 0.227216 6.1:+8860397 None:intergenic
TTTCCGTTGTTTGATAAGTA+TGG 0.248443 6.1:-8861010 MS.gene83911:CDS
GGAAACAACATTGGTCTTAC+TGG 0.254029 6.1:-8859517 MS.gene83911:CDS
TTCATGGATGAGCCAACTTC+TGG 0.262299 6.1:-8859582 MS.gene83911:CDS
TGAGGAAGTTATGGAACTTA+TGG 0.300971 6.1:-8859721 MS.gene83911:CDS
CCTCCACCTGGTGCATTCTA+TGG 0.302241 6.1:+8860384 None:intergenic
ACTTATCAAACAACGGAAAT+AGG 0.314806 6.1:+8861014 None:intergenic
GAGTAGGTATCGGAGATATT+TGG 0.327928 6.1:+8861180 None:intergenic
CACGGCGATGCTGCAGCGGC+GGG 0.354627 6.1:-8859460 MS.gene83911:CDS
TAATCCTTCAATAATATTCA+TGG 0.355171 6.1:-8859598 MS.gene83911:CDS
TCGCCGTGCACTTGATCTTA+TGG 0.364192 6.1:+8859475 None:intergenic
TATGGTCGAGTTCTTTGGCC+TGG 0.370910 6.1:+8860402 None:intergenic
GATCTTATGGCAGTGTATAC+AGG 0.383088 6.1:+8859488 None:intergenic
GCACGGCGATGCTGCAGCGG+CGG 0.447481 6.1:-8859461 MS.gene83911:CDS
GATGTTCATTGAGGAAGTTA+TGG 0.448837 6.1:-8859730 MS.gene83911:CDS
ATGGTCGAGTTCTTTGGCCT+GGG 0.450706 6.1:+8860403 None:intergenic
GCACTGCAAAACGCATTAGT+AGG 0.451716 6.1:-8859690 MS.gene83911:CDS
TCTCTTCAAGTTCCTCCACC+TGG 0.465182 6.1:+8860372 None:intergenic
AGACAGTAACATGGGGAGAA+TGG 0.471471 6.1:+8859896 None:intergenic
TCATGGATGAGCCAACTTCT+GGG 0.477286 6.1:-8859581 MS.gene83911:CDS
ATGTCTCACTTCGGTAGGTC+GGG 0.479067 6.1:-8861207 MS.gene83911:CDS
AAGCTTCCAAGGCTATTGAT+AGG 0.485732 6.1:-8860812 MS.gene83911:CDS
CATTGATGTCAAGGGACAAT+CGG 0.496450 6.1:+8859845 None:intergenic
ATCGAGCCTATCAATAGCCT+TGG 0.496893 6.1:+8860806 None:intergenic
TATTGTAATGAGAAGAGTTA+TGG 0.501543 6.1:-8859538 MS.gene83911:CDS
TGGTTTCAGCATTGATGTCA+AGG 0.516749 6.1:+8859836 None:intergenic
TCATCCATGAATATTATTGA+AGG 0.518364 6.1:+8859594 None:intergenic
CCAACTCAACTGCAATAGTC+AGG 0.527094 6.1:+8859625 None:intergenic
CATGTCTCACTTCGGTAGGT+CGG 0.527808 6.1:-8861208 MS.gene83911:CDS
CAACTTCTGGGCTAGATGCA+AGG 0.531166 6.1:-8859569 MS.gene83911:CDS
GTTGCAGCACATTGAAGCCC+AGG 0.531371 6.1:-8860420 MS.gene83911:CDS
GGTGGAGGAACTTGAAGAGA+AGG 0.533591 6.1:-8860369 MS.gene83911:CDS
CGGAAATAGGTCAGCAGCGG+TGG 0.537882 6.1:+8861027 None:intergenic
ATATGCTGATGAAGCTTCCA+AGG 0.553657 6.1:-8860823 MS.gene83911:CDS
AACTTCTGGGCTAGATGCAA+GGG 0.554605 6.1:-8859568 MS.gene83911:CDS
CCATAGAATGCACCAGGTGG+AGG 0.555814 6.1:-8860384 MS.gene83911:CDS
TTTAACACCGTCGATCTTTG+GGG 0.562379 6.1:+8860966 None:intergenic
CCTGACTATTGCAGTTGAGT+TGG 0.570806 6.1:-8859625 MS.gene83911:CDS
GTTGTTTGATAAGTATGGCA+AGG 0.571156 6.1:-8861005 MS.gene83911:CDS
AACGAGGCGAGAGTAGGTAT+CGG 0.572222 6.1:+8861170 None:intergenic
CATCAATGCTGAAACCAGAA+AGG 0.573881 6.1:-8859830 MS.gene83911:intron
GGTTTCAGCATTGATGTCAA+GGG 0.576052 6.1:+8859837 None:intergenic
CTTGCATCTAGCCCAGAAGT+TGG 0.583693 6.1:+8859570 None:intergenic
AAGAGTTATGGAAACAACAT+TGG 0.586854 6.1:-8859526 MS.gene83911:CDS
AGTGCACGGCGATGCTGCAG+CGG 0.588988 6.1:-8859464 MS.gene83911:CDS
AAAGCATGTCTCACTTCGGT+AGG 0.591319 6.1:-8861212 None:intergenic
GTTGAGAACGAGGCGAGAGT+AGG 0.591802 6.1:+8861164 None:intergenic
ACTTGAAGAGAAGGAGAGAG+TGG 0.596502 6.1:-8860360 MS.gene83911:CDS
AATCTTTGCTCTATTCAGCA+TGG 0.598977 6.1:-8859870 MS.gene83911:CDS
ATGCTTGTAGATGTTCATTG+AGG 0.599217 6.1:-8859739 MS.gene83911:intron
TAAAGGACTGGTGAATCGAG+AGG 0.602404 6.1:-8860867 MS.gene83911:intron
TAGGTATCGGAGATATTTGG+AGG 0.603733 6.1:+8861183 None:intergenic
AACGCATTAGTAGGACTACC+TGG 0.610421 6.1:-8859681 MS.gene83911:CDS
TTGCCATACTTATCAAACAA+CGG 0.633797 6.1:+8861007 None:intergenic
AAATAAAGCATGTCTCACTT+CGG 0.635826 6.1:-8861216 None:intergenic
AAGGCTATTGATAGGCTCGA+TGG 0.637906 6.1:-8860804 MS.gene83911:intron
ACTCGACCATAGAATGCACC+AGG 0.646539 6.1:-8860390 MS.gene83911:CDS
AGATTCATAGACAGTAACAT+GGG 0.652460 6.1:+8859888 None:intergenic
ACGGCGATGCTGCAGCGGCG+GGG 0.660691 6.1:-8859459 MS.gene83911:CDS
AAGATTCATAGACAGTAACA+TGG 0.664678 6.1:+8859887 None:intergenic
CTGCCATAAGATCAAGTGCA+CGG 0.666568 6.1:-8859478 MS.gene83911:CDS
TCTTCATCCCCAAAGATCGA+CGG 0.667294 6.1:-8860973 MS.gene83911:intron
GGAAAGTGATGTTGAGAACG+AGG 0.673806 6.1:+8861154 None:intergenic
CAACGGAAATAGGTCAGCAG+CGG 0.676990 6.1:+8861024 None:intergenic
GCTGAGAGACTACTAACACC+AGG 0.683951 6.1:+8859663 None:intergenic
CGACCATAGAATGCACCAGG+TGG 0.695799 6.1:-8860387 MS.gene83911:CDS
GATTCATAGACAGTAACATG+GGG 0.722326 6.1:+8859889 None:intergenic
TGGTGTTAGTAGTCTCTCAG+CGG 0.723328 6.1:-8859661 MS.gene83911:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGATAAATGAAAAAT+TGG + chr6.1:8859718-8859737 None:intergenic 10.0%
!! AATTTGATAAATGAAAAATT+GGG + chr6.1:8859717-8859736 None:intergenic 10.0%
!!! ATTTTTCTTGTATTTTTTAA+AGG - chr6.1:8859751-8859770 MS.gene83911:intron 10.0%
!! ATTTGATAAATGAAAAATTG+GGG + chr6.1:8859716-8859735 None:intergenic 15.0%
!! GTTAATTTATTTCGTTTATT+TGG - chr6.1:8859866-8859885 MS.gene83911:CDS 15.0%
!!! AAATGCACAATTTTATTTAT+TGG - chr6.1:8860578-8860597 MS.gene83911:intron 15.0%
!!! AATTTTATTTATTGGCAATT+TGG - chr6.1:8860586-8860605 MS.gene83911:intron 15.0%
!! AACATAATAATTCCAATGAA+TGG + chr6.1:8860858-8860877 None:intergenic 20.0%
!! ATAACATTAACATGTCTATA+GGG + chr6.1:8860118-8860137 None:intergenic 20.0%
!! TAATCCTTCAATAATATTCA+TGG - chr6.1:8861037-8861056 MS.gene83911:intron 20.0%
!! TATAACATTAACATGTCTAT+AGG + chr6.1:8860119-8860138 None:intergenic 20.0%
!! TTTGATAAATGAAAAATTGG+GGG + chr6.1:8859715-8859734 None:intergenic 20.0%
!!! TATTAAACTTTTAGCAAATG+TGG + chr6.1:8860322-8860341 None:intergenic 20.0%
! ATATTTGCTTAACTCCATTA+AGG - chr6.1:8860182-8860201 MS.gene83911:intron 25.0%
! ATCTTGAATAGACATAAACA+TGG + chr6.1:8860700-8860719 None:intergenic 25.0%
! CAGCATATATTCTAAACTAA+CGG + chr6.1:8860664-8860683 None:intergenic 25.0%
! CGTTTATTTGGAAGAAAAAA+AGG - chr6.1:8859878-8859897 MS.gene83911:CDS 25.0%
! GATTAAAATACTAACCTTTC+TGG + chr6.1:8860822-8860841 None:intergenic 25.0%
! TATTGTAATGAGAAGAGTTA+TGG - chr6.1:8861097-8861116 MS.gene83911:intron 25.0%
! TATTTGCTTAACTCCATTAA+GGG - chr6.1:8860183-8860202 MS.gene83911:intron 25.0%
! TCATCCATGAATATTATTGA+AGG + chr6.1:8861044-8861063 None:intergenic 25.0%
!!! AGGTTAGTATTTTAATCACT+TGG - chr6.1:8860825-8860844 MS.gene83911:CDS 25.0%
!!! GGTTAGTATTTTAATCACTT+GGG - chr6.1:8860826-8860845 MS.gene83911:CDS 25.0%
!!! TCTTGTATTTTTTAAAGGAC+TGG - chr6.1:8859756-8859775 MS.gene83911:intron 25.0%
AAGAGTTATGGAAACAACAT+TGG - chr6.1:8861109-8861128 MS.gene83911:intron 30.0%
AAGATTCATAGACAGTAACA+TGG + chr6.1:8860751-8860770 None:intergenic 30.0%
ACCTTCACAAATCACAATTA+AGG + chr6.1:8860350-8860369 None:intergenic 30.0%
ACTTATCAAACAACGGAAAT+AGG + chr6.1:8859624-8859643 None:intergenic 30.0%
AGATTCATAGACAGTAACAT+GGG + chr6.1:8860750-8860769 None:intergenic 30.0%
CCTTCACAAATCACAATTAA+GGG + chr6.1:8860349-8860368 None:intergenic 30.0%
TACCTAGAGGAAGTAAAATA+CGG + chr6.1:8859588-8859607 None:intergenic 30.0%
TTGCCATACTTATCAAACAA+CGG + chr6.1:8859631-8859650 None:intergenic 30.0%
TTTCCGTTGTTTGATAAGTA+TGG - chr6.1:8859625-8859644 MS.gene83911:CDS 30.0%
! AAATACGGTGTTAGGTTAAT+TGG + chr6.1:8859573-8859592 None:intergenic 30.0%
! AATACGGTGTTAGGTTAATT+GGG + chr6.1:8859572-8859591 None:intergenic 30.0%
! CGTTAGTTTAGAATATATGC+TGG - chr6.1:8860662-8860681 MS.gene83911:intron 30.0%
!! AGAATGTATGTTTTGTTGTG+TGG - chr6.1:8860392-8860411 MS.gene83911:CDS 30.0%
!! ATGTTTTGTTGTGTGGTTAT+GGG - chr6.1:8860399-8860418 MS.gene83911:CDS 30.0%
!! TATGTTTTGTTGTGTGGTTA+TGG - chr6.1:8860398-8860417 MS.gene83911:CDS 30.0%
!!! TTCTTGCTTTAGTTTTGCAA+TGG - chr6.1:8860615-8860634 MS.gene83911:intron 30.0%
AAACAGAGAGAAGAGAGAAA+AGG + chr6.1:8859518-8859537 None:intergenic 35.0%
AACAGAGAGAAGAGAGAAAA+GGG + chr6.1:8859517-8859536 None:intergenic 35.0%
AATCTTTGCTCTATTCAGCA+TGG - chr6.1:8860765-8860784 MS.gene83911:intron 35.0%
ACATGCATCTGCTTGATTTA+TGG - chr6.1:8860041-8860060 MS.gene83911:intron 35.0%
AGGAAGTAAAATACGGTGTT+AGG + chr6.1:8859581-8859600 None:intergenic 35.0%
ATGCTTGTAGATGTTCATTG+AGG - chr6.1:8860896-8860915 MS.gene83911:intron 35.0%
GATGTTCATTGAGGAAGTTA+TGG - chr6.1:8860905-8860924 MS.gene83911:intron 35.0%
GATTCATAGACAGTAACATG+GGG + chr6.1:8860749-8860768 None:intergenic 35.0%
GTTGTTTGATAAGTATGGCA+AGG - chr6.1:8859630-8859649 MS.gene83911:CDS 35.0%
TGAGGAAGTTATGGAACTTA+TGG - chr6.1:8860914-8860933 MS.gene83911:intron 35.0%
TGCTACTAATCTAGACAATG+TGG + chr6.1:8859975-8859994 None:intergenic 35.0%
! CCCTTAATTGTGATTTGTGA+AGG - chr6.1:8860346-8860365 MS.gene83911:intron 35.0%
! CTTGGGATTTTACCATTCAT+TGG - chr6.1:8860843-8860862 MS.gene83911:CDS 35.0%
! TTTTAACACCGTCGATCTTT+GGG + chr6.1:8859673-8859692 None:intergenic 35.0%
! TTTTTAACACCGTCGATCTT+TGG + chr6.1:8859674-8859693 None:intergenic 35.0%
!! TCTTACAAGTTTGCTATGTG+TGG - chr6.1:8860468-8860487 MS.gene83911:intron 35.0%
AAGCTTCCAAGGCTATTGAT+AGG - chr6.1:8859823-8859842 MS.gene83911:intron 40.0%
AGAGAAAAGGGTTTCGCTTA+CGG + chr6.1:8859505-8859524 None:intergenic 40.0%
ATATGCTGATGAAGCTTCCA+AGG - chr6.1:8859812-8859831 MS.gene83911:intron 40.0%
ATCTGCTTGATTTATGGTGG+AGG - chr6.1:8860047-8860066 MS.gene83911:intron 40.0%
CATCAATGCTGAAACCAGAA+AGG - chr6.1:8860805-8860824 MS.gene83911:CDS 40.0%
CATTCTATGGTCGAGTTCTT+TGG + chr6.1:8860241-8860260 None:intergenic 40.0%
CATTGATGTCAAGGGACAAT+CGG + chr6.1:8860793-8860812 None:intergenic 40.0%
GATCTTATGGCAGTGTATAC+AGG + chr6.1:8861150-8861169 None:intergenic 40.0%
GTAGCATGCAATAGCTTCTT+CGG - chr6.1:8859989-8860008 MS.gene83911:intron 40.0%
TGCATCTGCTTGATTTATGG+TGG - chr6.1:8860044-8860063 MS.gene83911:intron 40.0%
TTTAACACCGTCGATCTTTG+GGG + chr6.1:8859672-8859691 None:intergenic 40.0%
! CACCGTATTTTACTTCCTCT+AGG - chr6.1:8859583-8859602 MS.gene83911:CDS 40.0%
!! GAGTAGGTATCGGAGATATT+TGG + chr6.1:8859458-8859477 None:intergenic 40.0%
!! GGAAACAACATTGGTCTTAC+TGG - chr6.1:8861118-8861137 MS.gene83911:intron 40.0%
!! GGTTTCAGCATTGATGTCAA+GGG + chr6.1:8860801-8860820 None:intergenic 40.0%
!! TACAAGTTTGCTATGTGTGG+AGG - chr6.1:8860471-8860490 MS.gene83911:intron 40.0%
!! TAGGTATCGGAGATATTTGG+AGG + chr6.1:8859455-8859474 None:intergenic 40.0%
!! TGGTTTCAGCATTGATGTCA+AGG + chr6.1:8860802-8860821 None:intergenic 40.0%
AACGCATTAGTAGGACTACC+TGG - chr6.1:8860954-8860973 MS.gene83911:intron 45.0%
AAGGCTATTGATAGGCTCGA+TGG - chr6.1:8859831-8859850 MS.gene83911:CDS 45.0%
ACTTGAAGAGAAGGAGAGAG+TGG - chr6.1:8860275-8860294 MS.gene83911:intron 45.0%
AGACAGTAACATGGGGAGAA+TGG + chr6.1:8860742-8860761 None:intergenic 45.0%
ATCGAGCCTATCAATAGCCT+TGG + chr6.1:8859832-8859851 None:intergenic 45.0%
CAACTCAACTCTGCCCTTAA+TGG + chr6.1:8860199-8860218 None:intergenic 45.0%
CCAACTCAACTGCAATAGTC+AGG + chr6.1:8861013-8861032 None:intergenic 45.0%
CCTGACTATTGCAGTTGAGT+TGG - chr6.1:8861010-8861029 MS.gene83911:CDS 45.0%
CTGCCATAAGATCAAGTGCA+CGG - chr6.1:8861157-8861176 MS.gene83911:CDS 45.0%
GCACTGCAAAACGCATTAGT+AGG - chr6.1:8860945-8860964 MS.gene83911:intron 45.0%
GGAAAGTGATGTTGAGAACG+AGG + chr6.1:8859484-8859503 None:intergenic 45.0%
TCATGGATGAGCCAACTTCT+GGG - chr6.1:8861054-8861073 MS.gene83911:intron 45.0%
TCTTCATCCCCAAAGATCGA+CGG - chr6.1:8859662-8859681 MS.gene83911:CDS 45.0%
TTCATGGATGAGCCAACTTC+TGG - chr6.1:8861053-8861072 MS.gene83911:intron 45.0%
! AACTTCTGGGCTAGATGCAA+GGG - chr6.1:8861067-8861086 MS.gene83911:intron 45.0%
! AGTTTTGCAATGGTGCACTG+AGG - chr6.1:8860625-8860644 MS.gene83911:intron 45.0%
! GTTGTGTGGTTATGGGTTGT+GGG - chr6.1:8860406-8860425 MS.gene83911:CDS 45.0%
! GTTTTGCAATGGTGCACTGA+GGG - chr6.1:8860626-8860645 MS.gene83911:intron 45.0%
! TAAAGGACTGGTGAATCGAG+AGG - chr6.1:8859768-8859787 MS.gene83911:intron 45.0%
! TGGTGTTAGTAGTCTCTCAG+CGG - chr6.1:8860974-8860993 MS.gene83911:CDS 45.0%
! TGTTGTGTGGTTATGGGTTG+TGG - chr6.1:8860405-8860424 MS.gene83911:CDS 45.0%
!! GGTAATTTGCGAGAGGCATT+TGG - chr6.1:8860427-8860446 MS.gene83911:CDS 45.0%
AACGAGGCGAGAGTAGGTAT+CGG + chr6.1:8859468-8859487 None:intergenic 50.0%
ACTCGACCATAGAATGCACC+AGG - chr6.1:8860245-8860264 MS.gene83911:intron 50.0%
ATGGTCGAGTTCTTTGGCCT+GGG + chr6.1:8860235-8860254 None:intergenic 50.0%
ATGTCTCACTTCGGTAGGTC+GGG - chr6.1:8859428-8859447 MS.gene83911:CDS 50.0%
CAACGGAAATAGGTCAGCAG+CGG + chr6.1:8859614-8859633 None:intergenic 50.0%
CTTGCATCTAGCCCAGAAGT+TGG + chr6.1:8861068-8861087 None:intergenic 50.0%
GCTGAGAGACTACTAACACC+AGG + chr6.1:8860975-8860994 None:intergenic 50.0%
GGTGGAGGAACTTGAAGAGA+AGG - chr6.1:8860266-8860285 MS.gene83911:intron 50.0%
TATGGTCGAGTTCTTTGGCC+TGG + chr6.1:8860236-8860255 None:intergenic 50.0%
TCGCCGTGCACTTGATCTTA+TGG + chr6.1:8861163-8861182 None:intergenic 50.0%
! CAACTTCTGGGCTAGATGCA+AGG - chr6.1:8861066-8861085 MS.gene83911:intron 50.0%
! GGTTGTGGGTAATTTGCGAG+AGG - chr6.1:8860420-8860439 MS.gene83911:CDS 50.0%
! TCTCTTCAAGTTCCTCCACC+TGG + chr6.1:8860266-8860285 None:intergenic 50.0%
CCATAGAATGCACCAGGTGG+AGG - chr6.1:8860251-8860270 MS.gene83911:intron 55.0%
CCTCCACCTGGTGCATTCTA+TGG + chr6.1:8860254-8860273 None:intergenic 55.0%
CGACCATAGAATGCACCAGG+TGG - chr6.1:8860248-8860267 MS.gene83911:intron 55.0%
GTTGAGAACGAGGCGAGAGT+AGG + chr6.1:8859474-8859493 None:intergenic 55.0%
GTTGCAGCACATTGAAGCCC+AGG - chr6.1:8860215-8860234 MS.gene83911:intron 55.0%
CGGAAATAGGTCAGCAGCGG+TGG + chr6.1:8859611-8859630 None:intergenic 60.0%
TCAGCAGCGGTGGTACCTAG+AGG + chr6.1:8859601-8859620 None:intergenic 60.0%
AGTGCACGGCGATGCTGCAG+CGG - chr6.1:8861171-8861190 MS.gene83911:CDS 65.0%
ACGGCGATGCTGCAGCGGCG+GGG - chr6.1:8861176-8861195 MS.gene83911:CDS 75.0%
CACGGCGATGCTGCAGCGGC+GGG - chr6.1:8861175-8861194 MS.gene83911:CDS 75.0%
GCACGGCGATGCTGCAGCGG+CGG - chr6.1:8861174-8861193 MS.gene83911:CDS 75.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 8859428 8861229 8859428 ID=MS.gene83911
chr6.1 mRNA 8859428 8861229 8859428 ID=MS.gene83911.t1;Parent=MS.gene83911
chr6.1 exon 8861154 8861229 8861154 ID=MS.gene83911.t1.exon1;Parent=MS.gene83911.t1
chr6.1 CDS 8861154 8861229 8861154 ID=cds.MS.gene83911.t1;Parent=MS.gene83911.t1
chr6.1 exon 8860974 8861052 8860974 ID=MS.gene83911.t1.exon2;Parent=MS.gene83911.t1
chr6.1 CDS 8860974 8861052 8860974 ID=cds.MS.gene83911.t1;Parent=MS.gene83911.t1
chr6.1 exon 8860805 8860884 8860805 ID=MS.gene83911.t1.exon3;Parent=MS.gene83911.t1
chr6.1 CDS 8860805 8860884 8860805 ID=cds.MS.gene83911.t1;Parent=MS.gene83911.t1
chr6.1 exon 8860349 8860448 8860349 ID=MS.gene83911.t1.exon4;Parent=MS.gene83911.t1
chr6.1 CDS 8860349 8860448 8860349 ID=cds.MS.gene83911.t1;Parent=MS.gene83911.t1
chr6.1 exon 8859831 8859939 8859831 ID=MS.gene83911.t1.exon5;Parent=MS.gene83911.t1
chr6.1 CDS 8859831 8859939 8859831 ID=cds.MS.gene83911.t1;Parent=MS.gene83911.t1
chr6.1 exon 8859428 8859751 8859428 ID=MS.gene83911.t1.exon6;Parent=MS.gene83911.t1
chr6.1 CDS 8859428 8859751 8859428 ID=cds.MS.gene83911.t1;Parent=MS.gene83911.t1
Gene Sequence

>MS.gene83911

ATGTCTCACTTCGGTAGGTCGGGTCCTCCAAATATCTCCGATACCTACTCTCGCCTCGTTCTCAACATCACTTTCCGTAAGCGAAACCCTTTTCTCTCTTCTCTCTGTTTTCAATTCAATTTTTTTTCTTCTTATCTTTAACCCAATTAACCTAACACCGTATTTTACTTCCTCTAGGTACCACCGCTGCTGACCTATTTCCGTTGTTTGATAAGTATGGCAAGGTTGTTGACATCTTCATCCCCAAAGATCGACGGTGTTAAAAAAGTTTTTTTTTTTAATTTCCCCCAATTTTTCATTTATCAAATTTATTTATTTGTTGAATTTTTCTTGTATTTTTTAAAGGACTGGTGAATCGAGAGGTTTCGCTTTTGTGCGTTACAAATATGCTGATGAAGCTTCCAAGGCTATTGATAGGCTCGATGGTATATGCTTCTTGTTAATTTATTTCGTTTATTTGGAAGAAAAAAAGGTTTTTTTTAATGTGAATTTATAAAAATAAAAAAAAAGATTATTTTTTAATGACAATACTGTATGTTATGCTCCACATTGTCTAGATTAGTAGCATGCAATAGCTTCTTCGGAATTTTAAGATATATTAAATTATTGATTGACATGCATCTGCTTGATTTATGGTGGAGGTTTAGTGAACATGTTAATGTTATACTCTAGTATGATGAGTCAGAGCCCTATAGACATGTTAATGTTATACTTTAGTGAACATTCTACTGACGTCGTGATTCTTTTTCTTTGCATATTTGCTTAACTCCATTAAGGGCAGAGTTGAGTTGCAGCACATTGAAGCCCAGGCCAAAGAACTCGACCATAGAATGCACCAGGTGGAGGAACTTGAAGAGAAGGAGAGAGTGGCAAATGAAAATGTAAGCTTAACCACATTTGCTAAAAGTTTAATATGATCCCTTAATTGTGATTTGTGAAGGTTTTGTCGACTTTTTTATTTTGTAGAATGTATGTTTTGTTGTGTGGTTATGGGTTGTGGGTAATTTGCGAGAGGCATTTGGTGTCAATACTTTTTTATCTCTTACAAGTTTGCTATGTGTGGAGGATGTAACTGAATAAATTTCTAAGTTGCAGCTGATTGCATTATTCTATTCTCTATTTTTATTTTTCGTTATGCTCATATATATTTAAATGCACAATTTTATTTATTGGCAATTTGGCATGTTTTCTTGCTTTAGTTTTGCAATGGTGCACTGAGGGCTGCAAAATTTGCCGTTAGTTTAGAATATATGCTGGTTGCTTGTGATTCCATGTTTATGTCTATTCAAGATACTGTGAGCAAACTGATATCCATTCTCCCCATGTTACTGTCTATGAATCTTTGCTCTATTCAGCATGGCTCCGATTGTCCCTTGACATCAATGCTGAAACCAGAAAGGTTAGTATTTTAATCACTTGGGATTTTACCATTCATTGGAATTATTATGTTTAAGTTTGAAAATGACTTATGCTTGTAGATGTTCATTGAGGAAGTTATGGAACTTATGGAACTGAAAGCACTGCAAAACGCATTAGTAGGACTACCTGGTGTTAGTAGTCTCTCAGCGGAGCAGCACAAAAGCCTGACTATTGCAGTTGAGTTGGTTGCTAATCCTTCAATAATATTCATGGATGAGCCAACTTCTGGGCTAGATGCAAGGGATGCAGCTATTGTAATGAGAAGAGTTATGGAAACAACATTGGTCTTACTGGCGCAATCCTGTATACACTGCCATAAGATCAAGTGCACGGCGATGCTGCAGCGGCGGGGCTATAAGTTTGATTCCCTTTTTCTCCTTTAA
Protein sequence

>MS.gene83911.t1

MSHFGRSGPPNISDTYSRLVLNITFRTTAADLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAIDRLDELSCSTLKPRPKNSTIECTRWRNLKRRREWQMKIYCEQTDIHSPHVTVYESLLYSAWLRLSLDINAETRKMFIEEVMELMELKALQNALVGLPGVSSLSAEQHKSLTIAVELVANPSIIFMDEPTSGLDARDAAIVMRRVMETTLVLLAQSCIHCHKIKCTAMLQRRGYKFDSLFLL