Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83933.t1 | RHN55313.1 | 97.8 | 185 | 4 | 0 | 2 | 186 | 31 | 215 | 5.90E-98 | 367.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83933.t1 | Q8LDR3 | 84.0 | 187 | 30 | 0 | 2 | 188 | 37 | 223 | 9.6e-89 | 327.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83933.t1 | A0A396HS49 | 97.8 | 185 | 4 | 0 | 2 | 186 | 31 | 215 | 4.3e-98 | 367.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054584 | MS.gene83933 | 0.802439 | 5.61E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83933.t1 | MTR_5g039210 | 95.767 | 189 | 8 | 0 | 1 | 189 | 30 | 218 | 3.07e-135 | 379 |
| MS.gene83933.t1 | MTR_5g039210 | 98.089 | 157 | 3 | 0 | 1 | 157 | 30 | 186 | 6.65e-114 | 323 |
| MS.gene83933.t1 | MTR_8g036015 | 31.429 | 140 | 84 | 4 | 1 | 136 | 5 | 136 | 9.11e-13 | 64.3 |
| MS.gene83933.t1 | MTR_7g095750 | 36.800 | 125 | 61 | 5 | 33 | 152 | 59 | 170 | 7.10e-12 | 64.7 |
| MS.gene83933.t1 | MTR_7g105220 | 31.884 | 138 | 86 | 4 | 1 | 136 | 14 | 145 | 9.46e-12 | 61.6 |
| MS.gene83933.t1 | MTR_2g085075 | 31.690 | 142 | 85 | 4 | 1 | 138 | 466 | 599 | 1.04e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83933.t1 | AT1G26940 | 83.069 | 189 | 32 | 0 | 1 | 189 | 5 | 193 | 3.21e-120 | 340 |
| MS.gene83933.t1 | AT1G26940 | 83.069 | 189 | 32 | 0 | 1 | 189 | 36 | 224 | 1.42e-119 | 339 |
| MS.gene83933.t1 | AT3G44600 | 33.099 | 142 | 83 | 4 | 1 | 138 | 480 | 613 | 6.87e-13 | 67.8 |
| MS.gene83933.t1 | AT1G01940 | 31.429 | 140 | 84 | 4 | 1 | 136 | 5 | 136 | 5.53e-12 | 62.4 |
| MS.gene83933.t1 | AT2G36130 | 31.159 | 138 | 87 | 4 | 1 | 136 | 14 | 145 | 2.47e-11 | 60.5 |
| MS.gene83933.t1 | AT4G33060 | 28.000 | 200 | 115 | 6 | 1 | 192 | 17 | 195 | 5.96e-11 | 62.0 |
Find 51 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTTGTTCCTCATTCA+TGG | 0.236324 | 5.4:+29128738 | None:intergenic |
| TAACACCAATCATTTCTTTC+GGG | 0.252702 | 5.4:-29128938 | MS.gene83933:intron |
| TGACAAATATTCCCTCTTTA+CGG | 0.285365 | 5.4:+29128026 | None:intergenic |
| GAAAATGTGGTCAACTGTTT+TGG | 0.306456 | 5.4:+29128984 | None:intergenic |
| TCGTGAATGCAGACAAATTA+TGG | 0.318879 | 5.4:-29129042 | None:intergenic |
| ATAACACCAATCATTTCTTT+CGG | 0.318939 | 5.4:-29128939 | MS.gene83933:CDS |
| AATTATGGAGATATTGAGTT+TGG | 0.330974 | 5.4:-29129027 | MS.gene83933:CDS |
| AAAGAGGATCCACCACTATC+TGG | 0.341326 | 5.4:+29128232 | None:intergenic |
| CATTCGCAACATCTGCTACT+TGG | 0.346902 | 5.4:+29128773 | None:intergenic |
| GACAAATATTCCCTCTTTAC+GGG | 0.360829 | 5.4:+29128027 | None:intergenic |
| TCAACTTGATCGTATTGTTC+GGG | 0.373588 | 5.4:+29121226 | None:intergenic |
| CTTCTTTGTTCCTCATTCAT+GGG | 0.373755 | 5.4:+29128739 | None:intergenic |
| TTCAATGCTTAGGTAGATAA+GGG | 0.376717 | 5.4:-29128805 | MS.gene83933:intron |
| GGAAATTCTCCTCATCTTGA+TGG | 0.385053 | 5.4:-29128196 | MS.gene83933:CDS |
| ATCAACTTGATCGTATTGTT+CGG | 0.385876 | 5.4:+29121225 | None:intergenic |
| ATCCTCTCATCATATTACTA+TGG | 0.396619 | 5.4:-29126320 | MS.gene83933:intron |
| TACCTGTCTTTCAACTTCAA+TGG | 0.415502 | 5.4:+29124048 | None:intergenic |
| TTCAAGCTTGTGAGACTTGG+AGG | 0.430497 | 5.4:-29128964 | MS.gene83933:CDS |
| ATTCAATGCTTAGGTAGATA+AGG | 0.434823 | 5.4:-29128806 | MS.gene83933:intron |
| GAAGCTGAAAAGACAGTTGT+TGG | 0.436934 | 5.4:-29128715 | MS.gene83933:CDS |
| ATTCGCAACATCTGCTACTT+GGG | 0.441969 | 5.4:+29128774 | None:intergenic |
| TAGCGATGTCAAACATGTCC+GGG | 0.481197 | 5.4:-29128686 | MS.gene83933:CDS |
| TGTCCGGGGTATTCTTTCCA+TGG | 0.486863 | 5.4:-29128671 | MS.gene83933:CDS |
| TTAGCGATGTCAAACATGTC+CGG | 0.487431 | 5.4:-29128687 | MS.gene83933:CDS |
| TTCTCCTCATCTTGATGGAA+AGG | 0.495324 | 5.4:-29128191 | MS.gene83933:intron |
| AGCCATTGAAGTTGAAAGAC+AGG | 0.503275 | 5.4:-29124050 | MS.gene83933:intron |
| GTCTCACAAGCTTGAAAATG+TGG | 0.508296 | 5.4:+29128971 | None:intergenic |
| GGGGTATTCTTTCCATGGGA+AGG | 0.525012 | 5.4:-29128666 | MS.gene83933:intron |
| ATATTTGGAAAAGTTACTAA+AGG | 0.532245 | 5.4:-29128085 | MS.gene83933:CDS |
| GCAACTACCCACCCGTAAAG+AGG | 0.538995 | 5.4:-29128038 | MS.gene83933:CDS |
| GTCCGGGGTATTCTTTCCAT+GGG | 0.540623 | 5.4:-29128670 | MS.gene83933:CDS |
| AGGCACGACGATCCAGATAG+TGG | 0.560872 | 5.4:-29128244 | MS.gene83933:CDS |
| AGGATAGTGATGCGCTCCGT+TGG | 0.567136 | 5.4:+29126338 | None:intergenic |
| AAATATTCCCTCTTTACGGG+TGG | 0.567348 | 5.4:+29128030 | None:intergenic |
| ATGACACTGGTTATGCGGAT+AGG | 0.568014 | 5.4:-29121170 | MS.gene83933:CDS |
| CAGTACCCGAAAGAAATGAT+TGG | 0.581364 | 5.4:+29128933 | None:intergenic |
| CAACTACCCACCCGTAAAGA+GGG | 0.588030 | 5.4:-29128037 | MS.gene83933:CDS |
| AAGATCTGCTCCCATGAATG+AGG | 0.588977 | 5.4:-29128749 | MS.gene83933:CDS |
| TACCATAGTAATATGATGAG+AGG | 0.590982 | 5.4:+29126318 | None:intergenic |
| TAAGAAACCTAACCTTCCCA+TGG | 0.596016 | 5.4:+29128654 | None:intergenic |
| CAAGTAGCAGATGTTGCGAA+TGG | 0.600359 | 5.4:-29128772 | MS.gene83933:CDS |
| AATATTCCCTCTTTACGGGT+GGG | 0.615986 | 5.4:+29128031 | None:intergenic |
| TAAAGAGGGAATATTTGTCA+TGG | 0.617230 | 5.4:-29128023 | MS.gene83933:intron |
| CTCACCTTTCCATCAAGATG+AGG | 0.623174 | 5.4:+29128187 | None:intergenic |
| AGTTAATGACACTGGTTATG+CGG | 0.631899 | 5.4:-29121175 | MS.gene83933:CDS |
| GTGGAGGAAGTTAATGACAC+TGG | 0.644535 | 5.4:-29121183 | MS.gene83933:CDS |
| CACGACGATCCAGATAGTGG+TGG | 0.658792 | 5.4:-29128241 | MS.gene83933:CDS |
| GATAGGCCTTTATTACCAAG+TGG | 0.674110 | 5.4:-29121153 | MS.gene83933:CDS |
| AGCGATGTCAAACATGTCCG+GGG | 0.691354 | 5.4:-29128685 | MS.gene83933:CDS |
| TTCCCATGGAAAGAATACCC+CGG | 0.703104 | 5.4:+29128668 | None:intergenic |
| GCATTTGTTGTTAGACACGG+AGG | 0.708853 | 5.4:-29124125 | MS.gene83933:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 29121140 | 29129058 | 29121140 | ID=MS.gene83933 |
| chr5.4 | mRNA | 29121140 | 29129058 | 29121140 | ID=MS.gene83933.t1;Parent=MS.gene83933 |
| chr5.4 | exon | 29128939 | 29129058 | 29128939 | ID=MS.gene83933.t1.exon1;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29128939 | 29129058 | 29128939 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| chr5.4 | exon | 29128667 | 29128815 | 29128667 | ID=MS.gene83933.t1.exon2;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29128667 | 29128815 | 29128667 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| chr5.4 | exon | 29128192 | 29128264 | 29128192 | ID=MS.gene83933.t1.exon3;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29128192 | 29128264 | 29128192 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| chr5.4 | exon | 29128024 | 29128113 | 29128024 | ID=MS.gene83933.t1.exon4;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29128024 | 29128113 | 29128024 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| chr5.4 | exon | 29126321 | 29126360 | 29126321 | ID=MS.gene83933.t1.exon5;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29126321 | 29126360 | 29126321 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| chr5.4 | exon | 29124051 | 29124133 | 29124051 | ID=MS.gene83933.t1.exon6;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29124051 | 29124133 | 29124051 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| chr5.4 | exon | 29121140 | 29121265 | 29121140 | ID=MS.gene83933.t1.exon7;Parent=MS.gene83933.t1 |
| chr5.4 | CDS | 29121140 | 29121265 | 29121140 | ID=cds.MS.gene83933.t1;Parent=MS.gene83933.t1 |
| Gene Sequence |
| Protein sequence |
