Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84524.t1 | XP_021741128.1 | 67.3 | 315 | 84 | 2 | 1 | 315 | 1 | 296 | 9.50E-100 | 373.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84524.t1 | A0A1U8KII2 | 64.5 | 324 | 103 | 1 | 1 | 324 | 1 | 312 | 1.1e-94 | 356.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059272 | MS.gene84524 | 0.826472 | 2.66E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84524.t1 | MTR_7g062720 | 65.909 | 132 | 45 | 0 | 1 | 132 | 1 | 132 | 7.30e-60 | 190 |
| MS.gene84524.t1 | MTR_5g061150 | 62.992 | 127 | 47 | 0 | 20 | 146 | 10 | 136 | 1.39e-48 | 160 |
| MS.gene84524.t1 | MTR_4g037355 | 56.429 | 140 | 36 | 4 | 151 | 289 | 1 | 116 | 4.61e-42 | 143 |
| MS.gene84524.t1 | MTR_4g112730 | 69.863 | 73 | 20 | 1 | 138 | 210 | 1 | 71 | 6.34e-27 | 102 |
| MS.gene84524.t1 | MTR_1g036690 | 70.588 | 34 | 10 | 0 | 282 | 315 | 5 | 38 | 4.06e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 64 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGACTCTTAATATTTC+TGG | 0.080746 | 3.1:+45978132 | MS.gene84524:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 3.1:+45979025 | MS.gene84524:CDS |
| TATGGAAGGGGACGTGGTTT+TGG | 0.259444 | 3.1:+45978798 | MS.gene84524:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.269782 | 3.1:+45978322 | MS.gene84524:CDS |
| TCACTTCAAAATAAGGATAA+AGG | 0.276156 | 3.1:+45979032 | MS.gene84524:CDS |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 3.1:+45978146 | MS.gene84524:CDS |
| TGTGTTGCTACATTCACTTC+TGG | 0.294995 | 3.1:-45978696 | None:intergenic |
| ATGGTTGTATTTGCGTTTAC+AGG | 0.299146 | 3.1:+45978407 | MS.gene84524:CDS |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 3.1:-45979002 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 3.1:+45979084 | MS.gene84524:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 3.1:+45978151 | MS.gene84524:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.361577 | 3.1:+45978152 | MS.gene84524:CDS |
| CGTGGACGTGGTCGTGGTTA+TGG | 0.362984 | 3.1:+45978780 | MS.gene84524:CDS |
| TTTGGGAAAATTTAAAAGAA+AGG | 0.385472 | 3.1:+45978340 | MS.gene84524:CDS |
| AACCATTCGTTACGAGCATT+AGG | 0.390171 | 3.1:-45978390 | None:intergenic |
| AGAAGATCCAAAAGATCTTT+GGG | 0.393236 | 3.1:+45978323 | MS.gene84524:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.394855 | 3.1:+45979076 | MS.gene84524:CDS |
| GTGTTGCTACATTCACTTCT+GGG | 0.423307 | 3.1:-45978695 | None:intergenic |
| CGTGGTTATGGAAGGGGACG+TGG | 0.429618 | 3.1:+45978792 | MS.gene84524:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 3.1:+45978275 | MS.gene84524:CDS |
| CACTTCAAAATAAGGATAAA+GGG | 0.446661 | 3.1:+45979033 | MS.gene84524:CDS |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 3.1:+45978236 | MS.gene84524:CDS |
| CAAACTCGTGGTCGTGGTCG+TGG | 0.459890 | 3.1:+45978732 | MS.gene84524:CDS |
| AATTGTATCTCCATGCCCTT+CGG | 0.460742 | 3.1:-45978202 | None:intergenic |
| CGTGGTCGTGGTCGTGGCCA+TGG | 0.462334 | 3.1:+45978738 | MS.gene84524:CDS |
| GTTGTGGAGGCAAAGGTCAT+TGG | 0.462627 | 3.1:+45978961 | MS.gene84524:CDS |
| TTTCACGGTATTGTTGCTGC+AGG | 0.465441 | 3.1:-45978563 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 3.1:+45978903 | MS.gene84524:CDS |
| GCAGCAACAATACCGTGAAA+AGG | 0.474876 | 3.1:+45978566 | MS.gene84524:CDS |
| GCTACATTCACTTCTGGGAA+TGG | 0.483138 | 3.1:-45978690 | None:intergenic |
| ATTGACACTGTGTGGAGAAA+AGG | 0.483584 | 3.1:+45978482 | MS.gene84524:CDS |
| TGCTATCGTTGTGGAGGCAA+AGG | 0.489090 | 3.1:+45978954 | MS.gene84524:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 3.1:+45978192 | MS.gene84524:CDS |
| CAGCAACAATACCGTGAAAA+GGG | 0.498612 | 3.1:+45978567 | MS.gene84524:CDS |
| GACGTGGTCGTGGTTATGGA+AGG | 0.501950 | 3.1:+45978784 | MS.gene84524:CDS |
| GTAGCAACACATGATCACTA+TGG | 0.504040 | 3.1:+45978708 | MS.gene84524:CDS |
| TATGGACAAACTCGTGGTCG+TGG | 0.513531 | 3.1:+45978726 | MS.gene84524:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.515141 | 3.1:+45978186 | MS.gene84524:CDS |
| TACATTAGATGCATGAAAAG+TGG | 0.516304 | 3.1:-45978538 | None:intergenic |
| CGTGGCCATGGTCATGGTCG+TGG | 0.517086 | 3.1:+45978750 | MS.gene84524:CDS |
| CATGGTCGTGGACGTGGACG+TGG | 0.524744 | 3.1:+45978762 | MS.gene84524:CDS |
| CGTGGACGTGGACGTGGTCG+TGG | 0.524981 | 3.1:+45978774 | MS.gene84524:CDS |
| CGTGGTCGTGGCCATGGTCA+TGG | 0.529681 | 3.1:+45978744 | MS.gene84524:CDS |
| CATGGTCATGGTCGTGGACG+TGG | 0.530863 | 3.1:+45978756 | MS.gene84524:CDS |
| CATGGAGATACAATTAAAGA+AGG | 0.533844 | 3.1:+45978210 | MS.gene84524:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.536657 | 3.1:-45979114 | None:intergenic |
| CGTGGACGTGGACGTGGACG+TGG | 0.546682 | 3.1:+45978768 | MS.gene84524:CDS |
| CGTGGTCGTGGTTATGGAAG+GGG | 0.551769 | 3.1:+45978786 | MS.gene84524:CDS |
| TTCCTAATGCTCGTAACGAA+TGG | 0.575736 | 3.1:+45978388 | MS.gene84524:CDS |
| AACCACACGACTCGTCCCAC+TGG | 0.578009 | 3.1:+45978660 | MS.gene84524:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 3.1:+45978242 | MS.gene84524:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 3.1:+45978945 | MS.gene84524:CDS |
| ATTCGTTACGAGCATTAGGA+AGG | 0.589689 | 3.1:-45978386 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 3.1:-45978275 | None:intergenic |
| ACGTGGTCGTGGTTATGGAA+GGG | 0.598966 | 3.1:+45978785 | MS.gene84524:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.613176 | 3.1:+45978948 | MS.gene84524:CDS |
| AAATTCATTTAAATGCCGAA+GGG | 0.633517 | 3.1:+45978187 | MS.gene84524:CDS |
| CACGTCCACGACCATGACCA+TGG | 0.652278 | 3.1:-45978755 | None:intergenic |
| TACCAGTGGGACGAGTCGTG+TGG | 0.662791 | 3.1:-45978662 | None:intergenic |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 3.1:-45978265 | None:intergenic |
| ACTTCTCTATTGACACTGTG+TGG | 0.668278 | 3.1:+45978474 | MS.gene84524:CDS |
| TGGGAATGGAGCACTACCAG+TGG | 0.672955 | 3.1:-45978676 | None:intergenic |
| GGGAATGGAGCACTACCAGT+GGG | 0.676344 | 3.1:-45978675 | None:intergenic |
| GATCACTATGGACAAACTCG+TGG | 0.716183 | 3.1:+45978720 | MS.gene84524:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | + | chr3.1:45978903-45978922 | MS.gene84524:CDS | 20.0% |
| !! | TTTGGGAAAATTTAAAAGAA+AGG | + | chr3.1:45978340-45978359 | MS.gene84524:CDS | 20.0% |
| !!! | TAAATGAATTTTAGCATCAA+GGG | - | chr3.1:45978178-45978197 | None:intergenic | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | + | chr3.1:45978146-45978165 | MS.gene84524:CDS | 20.0% |
| !!! | TTAAATGAATTTTAGCATCA+AGG | - | chr3.1:45978179-45978198 | None:intergenic | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | + | chr3.1:45978186-45978205 | MS.gene84524:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | + | chr3.1:45978187-45978206 | MS.gene84524:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+GGG | + | chr3.1:45979033-45979052 | MS.gene84524:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | + | chr3.1:45979076-45979095 | MS.gene84524:CDS | 25.0% |
| ! | TCACTTCAAAATAAGGATAA+AGG | + | chr3.1:45979032-45979051 | MS.gene84524:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | + | chr3.1:45979084-45979103 | MS.gene84524:CDS | 25.0% |
| !! | AAATTTTCCCAAAGATCTTT+TGG | - | chr3.1:45978333-45978352 | None:intergenic | 25.0% |
| !! | ATCATTTTCACATGCAAAAT+GGG | - | chr3.1:45979065-45979084 | None:intergenic | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | - | chr3.1:45979005-45979024 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | - | chr3.1:45978893-45978912 | None:intergenic | 25.0% |
| !! | TTTGAGACTCTTAATATTTC+TGG | + | chr3.1:45978132-45978151 | MS.gene84524:CDS | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | - | chr3.1:45978290-45978309 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | - | chr3.1:45978869-45978888 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | + | chr3.1:45978323-45978342 | MS.gene84524:CDS | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | + | chr3.1:45978236-45978255 | MS.gene84524:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | + | chr3.1:45978210-45978229 | MS.gene84524:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | + | chr3.1:45979025-45979044 | MS.gene84524:CDS | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | - | chr3.1:45978541-45978560 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | + | chr3.1:45978322-45978341 | MS.gene84524:CDS | 30.0% | |
| ! | AAGATCTTTTGGATCTTCTA+TGG | - | chr3.1:45978322-45978341 | None:intergenic | 30.0% |
| ! | CATCATTTTCACATGCAAAA+TGG | - | chr3.1:45979066-45979085 | None:intergenic | 30.0% |
| ! | TCTCAAAATCAAGTTTTGCA+AGG | - | chr3.1:45978119-45978138 | None:intergenic | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | + | chr3.1:45978152-45978171 | MS.gene84524:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | - | chr3.1:45978287-45978306 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | + | chr3.1:45978819-45978838 | MS.gene84524:CDS | 30.0% |
| !!! | ACCTTTTAAAACCCTTTTCA+CGG | - | chr3.1:45978581-45978600 | None:intergenic | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | - | chr3.1:45979028-45979047 | None:intergenic | 30.0% |
| AACTGAACAACAAAAGGCTA+AGG | + | chr3.1:45978242-45978261 | MS.gene84524:CDS | 35.0% | |
| ATGGTTGTATTTGCGTTTAC+AGG | + | chr3.1:45978407-45978426 | MS.gene84524:CDS | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | + | chr3.1:45978151-45978170 | MS.gene84524:CDS | 35.0% | |
| ! | CTTTTGGATCTTCTATGGTA+AGG | - | chr3.1:45978317-45978336 | None:intergenic | 35.0% |
| !!! | ACCGTGAAAAGGGTTTTAAA+AGG | + | chr3.1:45978577-45978596 | MS.gene84524:CDS | 35.0% |
| AACCATTCGTTACGAGCATT+AGG | - | chr3.1:45978393-45978412 | None:intergenic | 40.0% | |
| AATTGTATCTCCATGCCCTT+CGG | - | chr3.1:45978205-45978224 | None:intergenic | 40.0% | |
| ACTTCTCTATTGACACTGTG+TGG | + | chr3.1:45978474-45978493 | MS.gene84524:CDS | 40.0% | |
| ATTCGTTACGAGCATTAGGA+AGG | - | chr3.1:45978389-45978408 | None:intergenic | 40.0% | |
| ATTGACACTGTGTGGAGAAA+AGG | + | chr3.1:45978482-45978501 | MS.gene84524:CDS | 40.0% | |
| CAGCAACAATACCGTGAAAA+GGG | + | chr3.1:45978567-45978586 | MS.gene84524:CDS | 40.0% | |
| GTAGCAACACATGATCACTA+TGG | + | chr3.1:45978708-45978727 | MS.gene84524:CDS | 40.0% | |
| TTCCTAATGCTCGTAACGAA+TGG | + | chr3.1:45978388-45978407 | MS.gene84524:CDS | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | - | chr3.1:45978371-45978390 | None:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | - | chr3.1:45978698-45978717 | None:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | - | chr3.1:45978699-45978718 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | + | chr3.1:45978948-45978967 | MS.gene84524:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | + | chr3.1:45978945-45978964 | MS.gene84524:CDS | 40.0% |
| !!! | AAGATGTTTTGGCGTACGAA+AGG | - | chr3.1:45978994-45979013 | None:intergenic | 40.0% |
| CATTTAAATGCCGAAGGGCA+TGG | + | chr3.1:45978192-45978211 | MS.gene84524:CDS | 45.0% | |
| GATCACTATGGACAAACTCG+TGG | + | chr3.1:45978720-45978739 | MS.gene84524:CDS | 45.0% | |
| GCAGCAACAATACCGTGAAA+AGG | + | chr3.1:45978566-45978585 | MS.gene84524:CDS | 45.0% | |
| TTTCACGGTATTGTTGCTGC+AGG | - | chr3.1:45978566-45978585 | None:intergenic | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | - | chr3.1:45978693-45978712 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | - | chr3.1:45978268-45978287 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | + | chr3.1:45978785-45978804 | MS.gene84524:CDS | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | + | chr3.1:45978275-45978294 | MS.gene84524:CDS | 50.0% | |
| GTTGTGGAGGCAAAGGTCAT+TGG | + | chr3.1:45978961-45978980 | MS.gene84524:CDS | 50.0% | |
| TATGGACAAACTCGTGGTCG+TGG | + | chr3.1:45978726-45978745 | MS.gene84524:CDS | 50.0% | |
| TGCTATCGTTGTGGAGGCAA+AGG | + | chr3.1:45978954-45978973 | MS.gene84524:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | - | chr3.1:45978278-45978297 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | + | chr3.1:45978798-45978817 | MS.gene84524:CDS | 50.0% |
| CGTGGTCGTGGTTATGGAAG+GGG | + | chr3.1:45978786-45978805 | MS.gene84524:CDS | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | + | chr3.1:45978784-45978803 | MS.gene84524:CDS | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | - | chr3.1:45978678-45978697 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | - | chr3.1:45978679-45978698 | None:intergenic | 55.0% |
| AACCACACGACTCGTCCCAC+TGG | + | chr3.1:45978660-45978679 | MS.gene84524:CDS | 60.0% | |
| CAAACTCGTGGTCGTGGTCG+TGG | + | chr3.1:45978732-45978751 | MS.gene84524:CDS | 60.0% | |
| CACGTCCACGACCATGACCA+TGG | - | chr3.1:45978758-45978777 | None:intergenic | 60.0% | |
| CATGGTCATGGTCGTGGACG+TGG | + | chr3.1:45978756-45978775 | MS.gene84524:CDS | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | + | chr3.1:45978780-45978799 | MS.gene84524:CDS | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | + | chr3.1:45978792-45978811 | MS.gene84524:CDS | 60.0% | |
| TACCAGTGGGACGAGTCGTG+TGG | - | chr3.1:45978665-45978684 | None:intergenic | 60.0% | |
| CATGGTCGTGGACGTGGACG+TGG | + | chr3.1:45978762-45978781 | MS.gene84524:CDS | 65.0% | |
| CGTGGCCATGGTCATGGTCG+TGG | + | chr3.1:45978750-45978769 | MS.gene84524:CDS | 65.0% | |
| CGTGGTCGTGGCCATGGTCA+TGG | + | chr3.1:45978744-45978763 | MS.gene84524:CDS | 65.0% | |
| CGTGGACGTGGACGTGGACG+TGG | + | chr3.1:45978768-45978787 | MS.gene84524:CDS | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | + | chr3.1:45978774-45978793 | MS.gene84524:CDS | 70.0% | |
| CGTGGTCGTGGTCGTGGCCA+TGG | + | chr3.1:45978738-45978757 | MS.gene84524:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 45978108 | 45979121 | 45978108 | ID=MS.gene84524 |
| chr3.1 | mRNA | 45978108 | 45979121 | 45978108 | ID=MS.gene84524.t1;Parent=MS.gene84524 |
| chr3.1 | exon | 45978108 | 45979121 | 45978108 | ID=MS.gene84524.t1.exon1;Parent=MS.gene84524.t1 |
| chr3.1 | CDS | 45978108 | 45979121 | 45978108 | ID=cds.MS.gene84524.t1;Parent=MS.gene84524.t1 |
| Gene Sequence |
| Protein sequence |
