Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050799 | MS.gene84542 | 0.82442 | 8.15E-54 | -1.69E-46 |
| MS.gene053900 | MS.gene84542 | 0.816081 | 6.65E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATACCTTTGTGAAGATAAA+AGG | 0.212182 | 3.1:-46240881 | None:intergenic |
| GTGATCTCATCCAGGATGAT+AGG | 0.356626 | 3.1:-46241572 | None:intergenic |
| CCAGGATGATAGGGTGGATA+GGG | 0.403549 | 3.1:-46241562 | None:intergenic |
| ATGTTGAAGGAGGCAAGAAC+TGG | 0.403774 | 3.1:+46241459 | MS.gene84542:CDS |
| ATAAAGGCGAGTACAAAGTT+GGG | 0.426432 | 3.1:+46239846 | MS.gene84542:CDS |
| TAAGGACGGGAAGGAACATT+TGG | 0.428961 | 3.1:+46241083 | MS.gene84542:CDS |
| TCTTATAAAATCATCATGAT+AGG | 0.434799 | 3.1:-46241634 | None:intergenic |
| GCAAGAGGTGTGATGGCAGC+AGG | 0.439246 | 3.1:-46241503 | None:intergenic |
| GAAGAAGCACGAGAAAATAA+AGG | 0.445989 | 3.1:+46239830 | MS.gene84542:CDS |
| AAATGTTCCTTCCCGTCCTT+AGG | 0.450368 | 3.1:-46241081 | None:intergenic |
| TGATGGCAGCAGGGGTAAGT+TGG | 0.456592 | 3.1:-46241493 | None:intergenic |
| CATACTACCTCTGCTAAGAT+TGG | 0.469905 | 3.1:+46241527 | MS.gene84542:CDS |
| AATAAAGGCGAGTACAAAGT+TGG | 0.474716 | 3.1:+46239845 | MS.gene84542:CDS |
| CCCTATCCACCCTATCATCC+TGG | 0.477480 | 3.1:+46241562 | MS.gene84542:CDS |
| ACCATGTTGCAGAATGTTGA+AGG | 0.481802 | 3.1:+46241446 | MS.gene84542:CDS |
| TAAGATAGTAACATATCCTA+AGG | 0.501123 | 3.1:+46241065 | MS.gene84542:intron |
| ATTCTGCAACATGGTGGAAG+GGG | 0.513075 | 3.1:-46241438 | None:intergenic |
| TTGCAGGAGTGATCTCATCC+AGG | 0.524797 | 3.1:-46241580 | None:intergenic |
| AAAATCATCATGATAGGAAG+AGG | 0.535692 | 3.1:-46241628 | None:intergenic |
| ACATTCTGCAACATGGTGGA+AGG | 0.539324 | 3.1:-46241440 | None:intergenic |
| TGATCTCATCCAGGATGATA+GGG | 0.550931 | 3.1:-46241571 | None:intergenic |
| TAGTAACATATCCTAAGGAC+GGG | 0.551671 | 3.1:+46241070 | MS.gene84542:CDS |
| TCCTTCAACATTCTGCAACA+TGG | 0.556193 | 3.1:-46241447 | None:intergenic |
| CATTCTGCAACATGGTGGAA+GGG | 0.560478 | 3.1:-46241439 | None:intergenic |
| AGTATGAGCAAGAGGTGTGA+TGG | 0.566077 | 3.1:-46241510 | None:intergenic |
| GATGGTCATTGTTGATGCTA+AGG | 0.581330 | 3.1:+46241107 | MS.gene84542:CDS |
| TCCAGGATGATAGGGTGGAT+AGG | 0.585429 | 3.1:-46241563 | None:intergenic |
| CAAGAGGTGTGATGGCAGCA+GGG | 0.589536 | 3.1:-46241502 | None:intergenic |
| GCAGAGGTAGTATGAGCAAG+AGG | 0.599567 | 3.1:-46241518 | None:intergenic |
| ATAGTAACATATCCTAAGGA+CGG | 0.602531 | 3.1:+46241069 | MS.gene84542:CDS |
| CGGGAAGGAACATTTGGAGA+TGG | 0.612772 | 3.1:+46241089 | MS.gene84542:CDS |
| TGTTGAAGGAGGCAAGAACT+GGG | 0.613431 | 3.1:+46241460 | MS.gene84542:CDS |
| TATGATTTAGGACACAAATG+TGG | 0.617365 | 3.1:+46241246 | MS.gene84542:intron |
| AACATATCCTAAGGACGGGA+AGG | 0.619664 | 3.1:+46241074 | MS.gene84542:CDS |
| CAGGATGATAGGGTGGATAG+GGG | 0.620934 | 3.1:-46241561 | None:intergenic |
| AAGAGGTGTGATGGCAGCAG+GGG | 0.623171 | 3.1:-46241501 | None:intergenic |
| TAAAGGCGAGTACAAAGTTG+GGG | 0.634627 | 3.1:+46239847 | MS.gene84542:CDS |
| TTCAACATTCTGCAACATGG+TGG | 0.658953 | 3.1:-46241444 | None:intergenic |
| TCTCATCCAGGATGATAGGG+TGG | 0.663515 | 3.1:-46241568 | None:intergenic |
| ACTTGAACCAATCTTAGCAG+AGG | 0.665354 | 3.1:-46241534 | None:intergenic |
| ATGTTGCAGAATGTTGAAGG+AGG | 0.685850 | 3.1:+46241449 | MS.gene84542:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAAAAAAGATTATAATT+AGG | + | chr3.1:46240386-46240405 | MS.gene84542:intron | 10.0% |
| !! | TATATTAGATCTTAAAAAAT+TGG | - | chr3.1:46240295-46240314 | None:intergenic | 10.0% |
| !!! | ATATTTTATGTATAAAACAA+TGG | + | chr3.1:46240175-46240194 | MS.gene84542:intron | 10.0% |
| !!! | TACATAAAGATTTTTTTTAA+AGG | - | chr3.1:46240207-46240226 | None:intergenic | 10.0% |
| !! | AAAAAAATCTTTATGTATTG+AGG | + | chr3.1:46240210-46240229 | MS.gene84542:intron | 15.0% |
| !!! | AACAGTAATTAATTCTTTTT+TGG | + | chr3.1:46239880-46239899 | MS.gene84542:intron | 15.0% |
| !! | AACAAAATCACATTTATCAA+AGG | - | chr3.1:46240501-46240520 | None:intergenic | 20.0% |
| !! | ACAATATACAATAGTAATAC+AGG | - | chr3.1:46240144-46240163 | None:intergenic | 20.0% |
| !! | ACATATGTTTAAACATATCA+AGG | - | chr3.1:46241355-46241374 | None:intergenic | 20.0% |
| !! | ATGTTATTATGCAGTTATTT+AGG | - | chr3.1:46241009-46241028 | None:intergenic | 20.0% |
| !! | CAAACTTATAACATGTTAAA+TGG | + | chr3.1:46240962-46240981 | MS.gene84542:intron | 20.0% |
| !! | TAAAAAAAAATAGCAGTTGA+TGG | - | chr3.1:46241047-46241066 | None:intergenic | 20.0% |
| !!! | TTAATTCTTTTTTGGCTTTT+AGG | + | chr3.1:46239888-46239907 | MS.gene84542:intron | 20.0% |
| !!! | TTTGATAAATGTGATTTTGT+TGG | + | chr3.1:46240500-46240519 | MS.gene84542:intron | 20.0% |
| ! | AAAATCTTTATGTATTGAGG+TGG | + | chr3.1:46240213-46240232 | MS.gene84542:intron | 25.0% |
| ! | AAGATGTCATAAATTAGTCA+AGG | - | chr3.1:46241168-46241187 | None:intergenic | 25.0% |
| ! | CACATTTATCAAAGGAAAAA+AGG | - | chr3.1:46240493-46240512 | None:intergenic | 25.0% |
| ! | GATATGTTCTGCAAATTTAT+TGG | + | chr3.1:46240100-46240119 | MS.gene84542:intron | 25.0% |
| ! | TAAGATAGTAACATATCCTA+AGG | + | chr3.1:46241065-46241084 | MS.gene84542:intron | 25.0% |
| ! | TCAATTAAGATGATCTACAT+TGG | + | chr3.1:46241312-46241331 | MS.gene84542:intron | 25.0% |
| !! | AAAAAACTCTTACTCTAAGA+TGG | + | chr3.1:46241601-46241620 | MS.gene84542:CDS | 25.0% |
| !! | AATTTGTAGTGTTTTCAACT+AGG | + | chr3.1:46240757-46240776 | MS.gene84542:intron | 25.0% |
| !! | ATTGTGTTGTTCTATGATTT+AGG | + | chr3.1:46241234-46241253 | MS.gene84542:intron | 25.0% |
| !! | TTTAGGATATGTACATATGA+AGG | - | chr3.1:46240992-46241011 | None:intergenic | 25.0% |
| !!! | CAACAAATCAAATGGTTTTA+AGG | - | chr3.1:46240570-46240589 | None:intergenic | 25.0% |
| !!! | CGAATATTTTTTTTCATGTG+CGG | - | chr3.1:46240413-46240432 | None:intergenic | 25.0% |
| ACATGCATCAACAAATCAAA+TGG | - | chr3.1:46240578-46240597 | None:intergenic | 30.0% | |
| ATAGTAACATATCCTAAGGA+CGG | + | chr3.1:46241069-46241088 | MS.gene84542:CDS | 30.0% | |
| CATACCTTTGTGAAGATAAA+AGG | - | chr3.1:46240884-46240903 | None:intergenic | 30.0% | |
| GATGCATGTTTGAAAGAATT+AGG | + | chr3.1:46240589-46240608 | MS.gene84542:intron | 30.0% | |
| GTGTGTTTGTTTGCTTATTA+GGG | + | chr3.1:46241192-46241211 | MS.gene84542:intron | 30.0% | |
| TATTAGGGTGATGATATACA+AGG | + | chr3.1:46241207-46241226 | MS.gene84542:intron | 30.0% | |
| TCAAGGAAATAATCAGTTGT+AGG | - | chr3.1:46241151-46241170 | None:intergenic | 30.0% | |
| TGTGTGTTTGTTTGCTTATT+AGG | + | chr3.1:46241191-46241210 | MS.gene84542:intron | 30.0% | |
| TTCTTGTGAGGAAACATAAT+TGG | - | chr3.1:46240451-46240470 | None:intergenic | 30.0% | |
| ! | ATATCATGTAAGCTCTCTTT+TGG | - | chr3.1:46240616-46240635 | None:intergenic | 30.0% |
| ! | CTACATTGGTTATTTATCTG+CGG | + | chr3.1:46241326-46241345 | MS.gene84542:intron | 30.0% |
| ! | TACTCCTTTTATCTTCACAA+AGG | + | chr3.1:46240877-46240896 | MS.gene84542:CDS | 30.0% |
| ! | TATGATTTAGGACACAAATG+TGG | + | chr3.1:46241246-46241265 | MS.gene84542:intron | 30.0% |
| ! | TTTTCTGTCCAATAAACCAA+GGG | - | chr3.1:46239999-46240018 | None:intergenic | 30.0% |
| ! | TTTTTCTGTCCAATAAACCA+AGG | - | chr3.1:46240000-46240019 | None:intergenic | 30.0% |
| !!! | TTAGAGTAAGAGTTTTTTGC+AGG | - | chr3.1:46241599-46241618 | None:intergenic | 30.0% |
| AAAGCTTTCCCTTGGTTTAT+TGG | + | chr3.1:46239988-46240007 | MS.gene84542:intron | 35.0% | |
| AATAAAGGCGAGTACAAAGT+TGG | + | chr3.1:46239845-46239864 | MS.gene84542:CDS | 35.0% | |
| AGAGAGCTTACATGATATGT+AGG | + | chr3.1:46240618-46240637 | MS.gene84542:intron | 35.0% | |
| ATAAAGGCGAGTACAAAGTT+GGG | + | chr3.1:46239846-46239865 | MS.gene84542:CDS | 35.0% | |
| TAAGAACATGATCTGTCACT+TGG | + | chr3.1:46241404-46241423 | MS.gene84542:intron | 35.0% | |
| TAGTAACATATCCTAAGGAC+GGG | + | chr3.1:46241070-46241089 | MS.gene84542:CDS | 35.0% | |
| TTGTATCTGTTGTTCTTGTG+AGG | - | chr3.1:46240463-46240482 | None:intergenic | 35.0% | |
| ! | GAAGAAGCACGAGAAAATAA+AGG | + | chr3.1:46239830-46239849 | MS.gene84542:CDS | 35.0% |
| !! | CAGTTGATGGATATGTACTT+AGG | - | chr3.1:46241034-46241053 | None:intergenic | 35.0% |
| ACCATGTTGCAGAATGTTGA+AGG | + | chr3.1:46241446-46241465 | MS.gene84542:CDS | 40.0% | |
| ACTTGAACCAATCTTAGCAG+AGG | - | chr3.1:46241537-46241556 | None:intergenic | 40.0% | |
| ATGTTGCAGAATGTTGAAGG+AGG | + | chr3.1:46241449-46241468 | MS.gene84542:CDS | 40.0% | |
| CATACTACCTCTGCTAAGAT+TGG | + | chr3.1:46241527-46241546 | MS.gene84542:CDS | 40.0% | |
| GATGGTCATTGTTGATGCTA+AGG | + | chr3.1:46241107-46241126 | MS.gene84542:CDS | 40.0% | |
| TAAAGGCGAGTACAAAGTTG+GGG | + | chr3.1:46239847-46239866 | MS.gene84542:CDS | 40.0% | |
| TCCATGAATGAGGTCGAAAA+TGG | + | chr3.1:46240661-46240680 | MS.gene84542:intron | 40.0% | |
| TCCTTCAACATTCTGCAACA+TGG | - | chr3.1:46241450-46241469 | None:intergenic | 40.0% | |
| TGATCTCATCCAGGATGATA+GGG | - | chr3.1:46241574-46241593 | None:intergenic | 40.0% | |
| TGTCTTTGAACAACAAGTGC+AGG | + | chr3.1:46240847-46240866 | MS.gene84542:intron | 40.0% | |
| TTCAACATTCTGCAACATGG+TGG | - | chr3.1:46241447-46241466 | None:intergenic | 40.0% | |
| ! | ACCATTTTCGACCTCATTCA+TGG | - | chr3.1:46240665-46240684 | None:intergenic | 40.0% |
| AAATGTTCCTTCCCGTCCTT+AGG | - | chr3.1:46241084-46241103 | None:intergenic | 45.0% | |
| AACATATCCTAAGGACGGGA+AGG | + | chr3.1:46241074-46241093 | MS.gene84542:CDS | 45.0% | |
| ATGTTGAAGGAGGCAAGAAC+TGG | + | chr3.1:46241459-46241478 | MS.gene84542:CDS | 45.0% | |
| GCTGTAGTACTCCATGAATG+AGG | + | chr3.1:46240651-46240670 | MS.gene84542:intron | 45.0% | |
| GTGATCTCATCCAGGATGAT+AGG | - | chr3.1:46241575-46241594 | None:intergenic | 45.0% | |
| GTTCTTGCAAAGCTTTCCCT+TGG | + | chr3.1:46239980-46239999 | MS.gene84542:intron | 45.0% | |
| TAAGGACGGGAAGGAACATT+TGG | + | chr3.1:46241083-46241102 | MS.gene84542:CDS | 45.0% | |
| TGTTGAAGGAGGCAAGAACT+GGG | + | chr3.1:46241460-46241479 | MS.gene84542:CDS | 45.0% | |
| ! | ACATTCTGCAACATGGTGGA+AGG | - | chr3.1:46241443-46241462 | None:intergenic | 45.0% |
| ! | AGTATGAGCAAGAGGTGTGA+TGG | - | chr3.1:46241513-46241532 | None:intergenic | 45.0% |
| ! | ATTCTGCAACATGGTGGAAG+GGG | - | chr3.1:46241441-46241460 | None:intergenic | 45.0% |
| ! | CATTCTGCAACATGGTGGAA+GGG | - | chr3.1:46241442-46241461 | None:intergenic | 45.0% |
| AAGTTGCAGATGCAGCACAC+TGG | - | chr3.1:46241379-46241398 | None:intergenic | 50.0% | |
| CAGGATGATAGGGTGGATAG+GGG | - | chr3.1:46241564-46241583 | None:intergenic | 50.0% | |
| CCAGGATGATAGGGTGGATA+GGG | - | chr3.1:46241565-46241584 | None:intergenic | 50.0% | |
| CGGGAAGGAACATTTGGAGA+TGG | + | chr3.1:46241089-46241108 | MS.gene84542:CDS | 50.0% | |
| GCAGAGGTAGTATGAGCAAG+AGG | - | chr3.1:46241521-46241540 | None:intergenic | 50.0% | |
| TCCAGGATGATAGGGTGGAT+AGG | - | chr3.1:46241566-46241585 | None:intergenic | 50.0% | |
| TCTCATCCAGGATGATAGGG+TGG | - | chr3.1:46241571-46241590 | None:intergenic | 50.0% | |
| TTGCAGGAGTGATCTCATCC+AGG | - | chr3.1:46241583-46241602 | None:intergenic | 50.0% | |
| CCCTATCCACCCTATCATCC+TGG | + | chr3.1:46241562-46241581 | MS.gene84542:CDS | 55.0% | |
| TGATGGCAGCAGGGGTAAGT+TGG | - | chr3.1:46241496-46241515 | None:intergenic | 55.0% | |
| ! | AAGAGGTGTGATGGCAGCAG+GGG | - | chr3.1:46241504-46241523 | None:intergenic | 55.0% |
| ! | CAAGAGGTGTGATGGCAGCA+GGG | - | chr3.1:46241505-46241524 | None:intergenic | 55.0% |
| ! | GCAAGAGGTGTGATGGCAGC+AGG | - | chr3.1:46241506-46241525 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 46239827 | 46241643 | 46239827 | ID=MS.gene84542 |
| chr3.1 | mRNA | 46239827 | 46241643 | 46239827 | ID=MS.gene84542.t1;Parent=MS.gene84542 |
| chr3.1 | exon | 46239827 | 46239883 | 46239827 | ID=MS.gene84542.t1.exon1;Parent=MS.gene84542.t1 |
| chr3.1 | CDS | 46239827 | 46239883 | 46239827 | ID=cds.MS.gene84542.t1;Parent=MS.gene84542.t1 |
| chr3.1 | exon | 46240869 | 46240898 | 46240869 | ID=MS.gene84542.t1.exon2;Parent=MS.gene84542.t1 |
| chr3.1 | CDS | 46240869 | 46240898 | 46240869 | ID=cds.MS.gene84542.t1;Parent=MS.gene84542.t1 |
| chr3.1 | exon | 46241069 | 46241128 | 46241069 | ID=MS.gene84542.t1.exon3;Parent=MS.gene84542.t1 |
| chr3.1 | CDS | 46241069 | 46241128 | 46241069 | ID=cds.MS.gene84542.t1;Parent=MS.gene84542.t1 |
| chr3.1 | exon | 46241256 | 46241294 | 46241256 | ID=MS.gene84542.t1.exon4;Parent=MS.gene84542.t1 |
| chr3.1 | CDS | 46241256 | 46241294 | 46241256 | ID=cds.MS.gene84542.t1;Parent=MS.gene84542.t1 |
| chr3.1 | exon | 46241431 | 46241643 | 46241431 | ID=MS.gene84542.t1.exon5;Parent=MS.gene84542.t1 |
| chr3.1 | CDS | 46241431 | 46241643 | 46241431 | ID=cds.MS.gene84542.t1;Parent=MS.gene84542.t1 |
| Gene Sequence |
| Protein sequence |
