Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85000.t1 | AGQ42774.1 | 100 | 175 | 0 | 0 | 1 | 175 | 161 | 335 | 4.30E-96 | 360.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85000.t1 | Q07185 | 96.6 | 175 | 6 | 0 | 1 | 175 | 147 | 321 | 9.1e-95 | 347.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85000.t1 | S5FNL5 | 100.0 | 175 | 0 | 0 | 1 | 175 | 161 | 335 | 3.1e-96 | 360.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055150 | MS.gene85000 | 0.804952 | 1.69E-49 | -1.69E-46 |
| MS.gene056530 | MS.gene85000 | 0.817167 | 3.79E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85000.t1 | MTR_5g026620 | 99.429 | 175 | 1 | 0 | 1 | 175 | 88 | 262 | 2.16e-129 | 363 |
| MS.gene85000.t1 | MTR_5g026620 | 99.429 | 175 | 1 | 0 | 1 | 175 | 156 | 330 | 3.09e-128 | 363 |
| MS.gene85000.t1 | MTR_4g094222 | 78.857 | 175 | 37 | 0 | 1 | 175 | 171 | 345 | 1.40e-104 | 303 |
| MS.gene85000.t1 | MTR_5g070680 | 75.429 | 175 | 43 | 0 | 1 | 175 | 149 | 323 | 1.90e-99 | 290 |
| MS.gene85000.t1 | MTR_5g070870 | 75.429 | 175 | 43 | 0 | 1 | 175 | 150 | 324 | 3.37e-93 | 274 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85000.t1 | AT3G22370 | 94.857 | 175 | 9 | 0 | 1 | 175 | 180 | 354 | 1.17e-122 | 350 |
| MS.gene85000.t1 | AT3G22360 | 90.857 | 175 | 16 | 0 | 1 | 175 | 151 | 325 | 8.41e-118 | 336 |
| MS.gene85000.t1 | AT3G27620 | 88.000 | 175 | 21 | 0 | 1 | 175 | 155 | 329 | 1.77e-113 | 325 |
| MS.gene85000.t1 | AT1G32350 | 79.429 | 175 | 36 | 0 | 1 | 175 | 144 | 318 | 2.67e-106 | 307 |
| MS.gene85000.t1 | AT5G64210 | 80.571 | 175 | 34 | 0 | 1 | 175 | 179 | 353 | 8.93e-106 | 307 |
| MS.gene85000.t1 | AT1G32350 | 80.769 | 156 | 30 | 0 | 1 | 156 | 144 | 299 | 3.93e-95 | 278 |
Find 46 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATACGGTGTGCAAATT+TGG | 0.112313 | 5.2:+17603200 | None:intergenic |
| TTCCTCAAAACTCTACTTTA+AGG | 0.145682 | 5.2:-17603065 | MS.gene85000:CDS |
| TTCTTCAATGCTTATTTCTT+AGG | 0.252741 | 5.2:-17603237 | MS.gene85000:CDS |
| CCACTTCCATGAAAGTCATT+AGG | 0.338652 | 5.2:+17603310 | None:intergenic |
| CAGCTATTGCTATTGACTAT+TGG | 0.356927 | 5.2:-17603092 | MS.gene85000:CDS |
| GGGATGTTGTCGAGGTTGTC+AGG | 0.363968 | 5.2:-17603044 | MS.gene85000:CDS |
| CATGCACCTAATGACTTTCA+TGG | 0.370912 | 5.2:-17603316 | MS.gene85000:CDS |
| ACCATACGGTGTGCAAATTT+GGG | 0.381391 | 5.2:+17603201 | None:intergenic |
| TCCTCAAAACTCTACTTTAA+GGG | 0.382420 | 5.2:-17603064 | MS.gene85000:CDS |
| AGGGAGGCTGCTGCTCCAAT+TGG | 0.436968 | 5.2:-17602667 | MS.gene85000:CDS |
| ATTACCAAGGACGAGAGTTA+AGG | 0.452922 | 5.2:-17602687 | MS.gene85000:CDS |
| TTACCAAGGACGAGAGTTAA+GGG | 0.457110 | 5.2:-17602686 | MS.gene85000:CDS |
| TGCACTTGTCATAACTGTCC+AGG | 0.463520 | 5.2:-17603265 | MS.gene85000:CDS |
| GGTATCTAGTGATAACCAAT+TGG | 0.473206 | 5.2:+17602652 | None:intergenic |
| AGCATTCCTCCTACCATACC+AGG | 0.499814 | 5.2:+17603420 | None:intergenic |
| GAGACAGTAGCTGCTGTCCC+TGG | 0.507998 | 5.2:-17603438 | MS.gene85000:CDS |
| ATCCACCACTGTGCTCGAAT+CGG | 0.508390 | 5.2:+17603376 | None:intergenic |
| ACCCAAATTTGCACACCGTA+TGG | 0.511597 | 5.2:-17603202 | MS.gene85000:CDS |
| CCTAATGACTTTCATGGAAG+TGG | 0.513462 | 5.2:-17603310 | MS.gene85000:CDS |
| TACTTTAAGGGATGTTGTCG+AGG | 0.527952 | 5.2:-17603052 | MS.gene85000:CDS |
| CCTCCCTTAACTCTCGTCCT+TGG | 0.532033 | 5.2:+17602683 | None:intergenic |
| AGTAGCTGCTGTCCCTGGTA+TGG | 0.538754 | 5.2:-17603433 | MS.gene85000:CDS |
| AGTGCACGCTCATACCACTT+TGG | 0.545912 | 5.2:+17603282 | None:intergenic |
| GGATGTTGTCGAGGTTGTCA+GGG | 0.547504 | 5.2:-17603043 | MS.gene85000:CDS |
| TCAATAGCAATAGCTGGAGC+AGG | 0.548905 | 5.2:+17603099 | None:intergenic |
| GCTGCTGTCCCTGGTATGGT+AGG | 0.561437 | 5.2:-17603429 | MS.gene85000:CDS |
| CGCCGATTCGAGCACAGTGG+TGG | 0.562853 | 5.2:-17603378 | MS.gene85000:CDS |
| AAATTTGCACACCGTATGGT+TGG | 0.563076 | 5.2:-17603198 | MS.gene85000:CDS |
| GTTTCTCAAGGAGCTTGACA+AGG | 0.563279 | 5.2:-17603139 | MS.gene85000:CDS |
| CTCATACACTGAGTTTCTCA+AGG | 0.566793 | 5.2:-17603151 | MS.gene85000:CDS |
| CAATAGTCAATAGCAATAGC+TGG | 0.570433 | 5.2:+17603093 | None:intergenic |
| GATTCGAGCACAGTGGTGGA+TGG | 0.584320 | 5.2:-17603374 | MS.gene85000:CDS |
| GCTGTCCCTGGTATGGTAGG+AGG | 0.587252 | 5.2:-17603426 | MS.gene85000:CDS |
| AGTGAATAGCTTCTTCCTCA+AGG | 0.587319 | 5.2:+17603172 | None:intergenic |
| CGATGTTAACCACTTTGCAT+CGG | 0.619490 | 5.2:-17603001 | MS.gene85000:intron |
| GCACTTGTCATAACTGTCCA+GGG | 0.622780 | 5.2:-17603264 | MS.gene85000:CDS |
| CCTCAAGGTAACCAACCATA+CGG | 0.624191 | 5.2:+17603187 | None:intergenic |
| GACAACATACCGATGCAAAG+TGG | 0.644093 | 5.2:+17602992 | None:intergenic |
| CCGTATGGTTGGTTACCTTG+AGG | 0.649479 | 5.2:-17603187 | MS.gene85000:CDS |
| TTTCTCAAGGAGCTTGACAA+GGG | 0.653346 | 5.2:-17603138 | MS.gene85000:CDS |
| TGGAAGTGGCAAAACCAAAG+TGG | 0.670752 | 5.2:-17603296 | MS.gene85000:CDS |
| AAGCATTGAAGAAAACACCC+TGG | 0.672042 | 5.2:+17603247 | None:intergenic |
| TTGCAGGATATACATTACCA+AGG | 0.701646 | 5.2:-17602700 | MS.gene85000:intron |
| GCATTCCTCCTACCATACCA+GGG | 0.715449 | 5.2:+17603421 | None:intergenic |
| CCAAGGACGAGAGTTAAGGG+AGG | 0.747025 | 5.2:-17602683 | MS.gene85000:CDS |
| TTGCGCCGATTCGAGCACAG+TGG | 0.770199 | 5.2:-17603381 | MS.gene85000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAGCTTATAGATAAATAAA+TGG | + | chr5.2:17603169-17603188 | None:intergenic | 20.0% |
| ! | CATAATAAAAAGACAGATCA+TGG | + | chr5.2:17603219-17603238 | None:intergenic | 25.0% |
| !! | TTCTTCAATGCTTATTTCTT+AGG | - | chr5.2:17602867-17602886 | MS.gene85000:intron | 25.0% |
| !! | TTTCTAAAAAGACACAACTA+TGG | + | chr5.2:17603307-17603326 | None:intergenic | 25.0% |
| !!! | TAGTTGTGTCTTTTTAGAAA+TGG | - | chr5.2:17603307-17603326 | MS.gene85000:CDS | 25.0% |
| !!! | TTCATCTGAGTATTTTGAAT+GGG | - | chr5.2:17603346-17603365 | MS.gene85000:CDS | 25.0% |
| CATTCAAAATACTCAGATGA+AGG | + | chr5.2:17603347-17603366 | None:intergenic | 30.0% | |
| CTATAAGCTACAAATTCAAG+AGG | - | chr5.2:17603178-17603197 | MS.gene85000:CDS | 30.0% | |
| TCCTCAAAACTCTACTTTAA+GGG | - | chr5.2:17603040-17603059 | MS.gene85000:CDS | 30.0% | |
| TTCCTCAAAACTCTACTTTA+AGG | - | chr5.2:17603039-17603058 | MS.gene85000:CDS | 30.0% | |
| ! | GTTTTCATGTAATGAATTGC+AGG | - | chr5.2:17603388-17603407 | MS.gene85000:CDS | 30.0% |
| !! | CTTCATCTGAGTATTTTGAA+TGG | - | chr5.2:17603345-17603364 | MS.gene85000:CDS | 30.0% |
| CAATAGTCAATAGCAATAGC+TGG | + | chr5.2:17603014-17603033 | None:intergenic | 35.0% | |
| TTGCAGGATATACATTACCA+AGG | - | chr5.2:17603404-17603423 | MS.gene85000:CDS | 35.0% | |
| ! | CAGCTATTGCTATTGACTAT+TGG | - | chr5.2:17603012-17603031 | MS.gene85000:CDS | 35.0% |
| ! | TCCCTTAAAGTAGAGTTTTG+AGG | + | chr5.2:17603044-17603063 | None:intergenic | 35.0% |
| AAATTTGCACACCGTATGGT+TGG | - | chr5.2:17602906-17602925 | MS.gene85000:intron | 40.0% | |
| AACCATACGGTGTGCAAATT+TGG | + | chr5.2:17602907-17602926 | None:intergenic | 40.0% | |
| ACCATACGGTGTGCAAATTT+GGG | + | chr5.2:17602906-17602925 | None:intergenic | 40.0% | |
| AGTGAATAGCTTCTTCCTCA+AGG | + | chr5.2:17602935-17602954 | None:intergenic | 40.0% | |
| ATTACCAAGGACGAGAGTTA+AGG | - | chr5.2:17603417-17603436 | MS.gene85000:CDS | 40.0% | |
| CATGCACCTAATGACTTTCA+TGG | - | chr5.2:17602788-17602807 | MS.gene85000:intron | 40.0% | |
| CCACTTCCATGAAAGTCATT+AGG | + | chr5.2:17602797-17602816 | None:intergenic | 40.0% | |
| CCTAATGACTTTCATGGAAG+TGG | - | chr5.2:17602794-17602813 | MS.gene85000:intron | 40.0% | |
| CGATGTTAACCACTTTGCAT+CGG | - | chr5.2:17603103-17603122 | MS.gene85000:CDS | 40.0% | |
| CTCATACACTGAGTTTCTCA+AGG | - | chr5.2:17602953-17602972 | MS.gene85000:intron | 40.0% | |
| TTACCAAGGACGAGAGTTAA+GGG | - | chr5.2:17603418-17603437 | MS.gene85000:CDS | 40.0% | |
| TTTCTCAAGGAGCTTGACAA+GGG | - | chr5.2:17602966-17602985 | MS.gene85000:intron | 40.0% | |
| ! | TACTTTAAGGGATGTTGTCG+AGG | - | chr5.2:17603052-17603071 | MS.gene85000:CDS | 40.0% |
| !! | AAGCATTGAAGAAAACACCC+TGG | + | chr5.2:17602860-17602879 | None:intergenic | 40.0% |
| ACCCAAATTTGCACACCGTA+TGG | - | chr5.2:17602902-17602921 | MS.gene85000:intron | 45.0% | |
| ATTGCGCAAGAGTGAGACAA+AGG | + | chr5.2:17603142-17603161 | None:intergenic | 45.0% | |
| CCTCAAGGTAACCAACCATA+CGG | + | chr5.2:17602920-17602939 | None:intergenic | 45.0% | |
| GACAACATACCGATGCAAAG+TGG | + | chr5.2:17603115-17603134 | None:intergenic | 45.0% | |
| GCACTTGTCATAACTGTCCA+GGG | - | chr5.2:17602840-17602859 | MS.gene85000:intron | 45.0% | |
| GTTTCTCAAGGAGCTTGACA+AGG | - | chr5.2:17602965-17602984 | MS.gene85000:intron | 45.0% | |
| TCAATAGCAATAGCTGGAGC+AGG | + | chr5.2:17603008-17603027 | None:intergenic | 45.0% | |
| TGCACTTGTCATAACTGTCC+AGG | - | chr5.2:17602839-17602858 | MS.gene85000:intron | 45.0% | |
| TGGAAGTGGCAAAACCAAAG+TGG | - | chr5.2:17602808-17602827 | MS.gene85000:intron | 45.0% | |
| TTGCGCAAGAGTGAGACAAA+GGG | + | chr5.2:17603141-17603160 | None:intergenic | 45.0% | |
| AGCATTCCTCCTACCATACC+AGG | + | chr5.2:17602687-17602706 | None:intergenic | 50.0% | |
| AGTGCACGCTCATACCACTT+TGG | + | chr5.2:17602825-17602844 | None:intergenic | 50.0% | |
| ATCCACCACTGTGCTCGAAT+CGG | + | chr5.2:17602731-17602750 | None:intergenic | 50.0% | |
| GCATTCCTCCTACCATACCA+GGG | + | chr5.2:17602686-17602705 | None:intergenic | 50.0% | |
| GGATGTTGTCGAGGTTGTCA+GGG | - | chr5.2:17603061-17603080 | MS.gene85000:CDS | 50.0% | |
| ! | CCGTATGGTTGGTTACCTTG+AGG | - | chr5.2:17602917-17602936 | MS.gene85000:intron | 50.0% |
| AGTAGCTGCTGTCCCTGGTA+TGG | - | chr5.2:17602671-17602690 | MS.gene85000:CDS | 55.0% | |
| CCAAGGACGAGAGTTAAGGG+AGG | - | chr5.2:17603421-17603440 | MS.gene85000:CDS | 55.0% | |
| CCTCCCTTAACTCTCGTCCT+TGG | + | chr5.2:17603424-17603443 | None:intergenic | 55.0% | |
| GGGATGTTGTCGAGGTTGTC+AGG | - | chr5.2:17603060-17603079 | MS.gene85000:CDS | 55.0% | |
| ! | GATTCGAGCACAGTGGTGGA+TGG | - | chr5.2:17602730-17602749 | MS.gene85000:intron | 55.0% |
| AGGGAGGCTGCTGCTCCAAT+TGG | - | chr5.2:17603437-17603456 | MS.gene85000:CDS | 60.0% | |
| GAGACAGTAGCTGCTGTCCC+TGG | - | chr5.2:17602666-17602685 | MS.gene85000:CDS | 60.0% | |
| GCTGCTGTCCCTGGTATGGT+AGG | - | chr5.2:17602675-17602694 | MS.gene85000:CDS | 60.0% | |
| GCTGTCCCTGGTATGGTAGG+AGG | - | chr5.2:17602678-17602697 | MS.gene85000:CDS | 60.0% | |
| !! | TTGCGCCGATTCGAGCACAG+TGG | - | chr5.2:17602723-17602742 | MS.gene85000:intron | 60.0% |
| !! | CGCCGATTCGAGCACAGTGG+TGG | - | chr5.2:17602726-17602745 | MS.gene85000:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 17602657 | 17603469 | 17602657 | ID=MS.gene85000 |
| chr5.2 | mRNA | 17602657 | 17603469 | 17602657 | ID=MS.gene85000.t1;Parent=MS.gene85000 |
| chr5.2 | exon | 17603002 | 17603469 | 17603002 | ID=MS.gene85000.t1.exon1;Parent=MS.gene85000.t1 |
| chr5.2 | CDS | 17603002 | 17603469 | 17603002 | ID=cds.MS.gene85000.t1;Parent=MS.gene85000.t1 |
| chr5.2 | exon | 17602657 | 17602716 | 17602657 | ID=MS.gene85000.t1.exon2;Parent=MS.gene85000.t1 |
| chr5.2 | CDS | 17602657 | 17602716 | 17602657 | ID=cds.MS.gene85000.t1;Parent=MS.gene85000.t1 |
| Gene Sequence |
| Protein sequence |
