Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85501.t1 | RHN65795.1 | 85.3 | 389 | 54 | 2 | 1 | 387 | 54 | 441 | 6.90E-179 | 636.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85501.t1 | Q9S7T8 | 53.7 | 391 | 174 | 4 | 1 | 385 | 1 | 390 | 1.2e-115 | 417.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85501.t1 | G7IW05 | 85.3 | 389 | 54 | 2 | 1 | 387 | 1 | 388 | 5.0e-179 | 636.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene065945 | MS.gene85501 | PPI |
| MS.gene85501 | MS.gene045585 | PPI |
| MS.gene006610 | MS.gene85501 | PPI |
| MS.gene031697 | MS.gene85501 | PPI |
| MS.gene85501 | MS.gene065944 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85501.t1 | MTR_3g018740 | 85.347 | 389 | 54 | 2 | 1 | 387 | 1 | 388 | 0.0 | 657 |
| MS.gene85501.t1 | MTR_3g101010 | 72.680 | 388 | 102 | 2 | 1 | 385 | 1 | 387 | 0.0 | 550 |
| MS.gene85501.t1 | MTR_2g045980 | 69.975 | 393 | 107 | 5 | 1 | 387 | 1 | 388 | 0.0 | 523 |
| MS.gene85501.t1 | MTR_8g027395 | 68.912 | 386 | 112 | 3 | 3 | 385 | 15 | 395 | 0.0 | 523 |
| MS.gene85501.t1 | MTR_4g045707 | 69.191 | 383 | 116 | 2 | 4 | 385 | 5 | 386 | 0.0 | 523 |
| MS.gene85501.t1 | MTR_2g046030 | 67.939 | 393 | 115 | 5 | 1 | 387 | 1 | 388 | 0.0 | 513 |
| MS.gene85501.t1 | MTR_3g048000 | 65.535 | 383 | 113 | 3 | 6 | 387 | 28 | 392 | 2.29e-177 | 499 |
| MS.gene85501.t1 | MTR_3g101030 | 64.736 | 397 | 116 | 5 | 10 | 385 | 34 | 427 | 3.58e-176 | 497 |
| MS.gene85501.t1 | MTR_4g088525 | 68.508 | 362 | 112 | 2 | 24 | 385 | 8 | 367 | 7.33e-176 | 494 |
| MS.gene85501.t1 | MTR_8g461160 | 66.923 | 390 | 121 | 4 | 5 | 387 | 6 | 394 | 8.14e-176 | 495 |
| MS.gene85501.t1 | MTR_3g101180 | 65.000 | 400 | 123 | 8 | 1 | 385 | 1 | 398 | 1.59e-175 | 494 |
| MS.gene85501.t1 | MTR_8g027420 | 66.839 | 386 | 109 | 4 | 3 | 385 | 4 | 373 | 3.00e-175 | 493 |
| MS.gene85501.t1 | MTR_7g451890 | 62.916 | 391 | 133 | 4 | 5 | 386 | 14 | 401 | 1.17e-171 | 485 |
| MS.gene85501.t1 | MTR_7g050750 | 63.613 | 393 | 131 | 5 | 3 | 386 | 6 | 395 | 2.13e-171 | 484 |
| MS.gene85501.t1 | MTR_7g047780 | 63.427 | 391 | 131 | 5 | 5 | 386 | 14 | 401 | 3.39e-171 | 484 |
| MS.gene85501.t1 | MTR_3g101130 | 63.402 | 388 | 131 | 4 | 4 | 385 | 5 | 387 | 3.94e-170 | 481 |
| MS.gene85501.t1 | MTR_4g088535 | 66.138 | 378 | 110 | 3 | 5 | 382 | 7 | 366 | 4.94e-168 | 475 |
| MS.gene85501.t1 | MTR_7g050810 | 61.637 | 391 | 142 | 3 | 3 | 386 | 14 | 403 | 1.95e-167 | 474 |
| MS.gene85501.t1 | MTR_3g101050 | 62.953 | 386 | 114 | 6 | 8 | 385 | 32 | 396 | 1.17e-156 | 447 |
| MS.gene85501.t1 | MTR_3g015620 | 60.925 | 389 | 120 | 4 | 5 | 386 | 16 | 379 | 5.58e-155 | 442 |
| MS.gene85501.t1 | MTR_3g018747 | 81.609 | 261 | 32 | 2 | 129 | 387 | 9 | 255 | 6.61e-148 | 419 |
| MS.gene85501.t1 | MTR_3g102920 | 55.838 | 394 | 125 | 7 | 5 | 385 | 51 | 408 | 6.86e-136 | 394 |
| MS.gene85501.t1 | MTR_3g101190 | 55.584 | 385 | 96 | 6 | 1 | 385 | 1 | 310 | 2.84e-132 | 381 |
| MS.gene85501.t1 | MTR_3g101200 | 53.866 | 388 | 148 | 5 | 3 | 385 | 29 | 390 | 4.07e-126 | 369 |
| MS.gene85501.t1 | MTR_3g100995 | 65.625 | 256 | 83 | 2 | 133 | 386 | 11 | 263 | 3.77e-118 | 344 |
| MS.gene85501.t1 | MTR_3g100010 | 58.013 | 312 | 83 | 3 | 28 | 306 | 40 | 336 | 9.10e-117 | 343 |
| MS.gene85501.t1 | MTR_7g050830 | 56.688 | 314 | 103 | 3 | 5 | 296 | 16 | 318 | 3.57e-116 | 341 |
| MS.gene85501.t1 | MTR_3g015760 | 62.879 | 264 | 88 | 4 | 128 | 386 | 22 | 280 | 6.52e-105 | 311 |
| MS.gene85501.t1 | MTR_3g111160 | 70.320 | 219 | 61 | 3 | 170 | 385 | 1 | 218 | 1.22e-101 | 300 |
| MS.gene85501.t1 | MTR_3g114310 | 43.257 | 393 | 204 | 10 | 6 | 384 | 20 | 407 | 5.56e-92 | 283 |
| MS.gene85501.t1 | MTR_3g100520 | 62.212 | 217 | 71 | 3 | 170 | 385 | 1 | 207 | 4.02e-86 | 260 |
| MS.gene85501.t1 | MTR_2g046410 | 66.346 | 208 | 62 | 4 | 186 | 387 | 144 | 349 | 3.31e-85 | 263 |
| MS.gene85501.t1 | MTR_3g101110 | 57.322 | 239 | 70 | 3 | 129 | 361 | 68 | 280 | 1.63e-84 | 259 |
| MS.gene85501.t1 | MTR_3g099970 | 63.547 | 203 | 56 | 3 | 123 | 325 | 14 | 198 | 1.43e-82 | 251 |
| MS.gene85501.t1 | MTR_3g111150 | 63.277 | 177 | 41 | 1 | 7 | 183 | 1 | 153 | 7.59e-68 | 212 |
| MS.gene85501.t1 | MTR_3g101020 | 54.301 | 186 | 56 | 3 | 203 | 386 | 1 | 159 | 3.02e-58 | 187 |
| MS.gene85501.t1 | MTR_3g102870 | 58.642 | 162 | 63 | 2 | 192 | 350 | 1 | 161 | 3.31e-57 | 185 |
| MS.gene85501.t1 | MTR_3g101150 | 58.861 | 158 | 57 | 3 | 235 | 385 | 1 | 157 | 1.06e-55 | 180 |
| MS.gene85501.t1 | MTR_3g100990 | 62.987 | 154 | 56 | 1 | 1 | 154 | 1 | 153 | 3.91e-54 | 187 |
| MS.gene85501.t1 | MTR_4g093550 | 70.238 | 84 | 25 | 0 | 5 | 88 | 6 | 89 | 1.06e-31 | 115 |
| MS.gene85501.t1 | MTR_6g046890 | 65.753 | 73 | 25 | 0 | 13 | 85 | 7 | 79 | 5.68e-23 | 94.0 |
| MS.gene85501.t1 | MTR_3g101140 | 55.405 | 74 | 28 | 2 | 316 | 385 | 42 | 114 | 1.90e-15 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85501.t1 | AT1G47710 | 53.708 | 391 | 174 | 4 | 1 | 385 | 1 | 390 | 1.07e-149 | 429 |
| MS.gene85501.t1 | AT1G47710 | 53.708 | 391 | 174 | 4 | 1 | 385 | 28 | 417 | 6.83e-149 | 428 |
| MS.gene85501.t1 | AT3G45220 | 49.109 | 393 | 188 | 5 | 1 | 384 | 1 | 390 | 2.14e-127 | 372 |
| MS.gene85501.t1 | AT2G25240 | 49.227 | 388 | 187 | 5 | 1 | 384 | 1 | 382 | 4.84e-126 | 369 |
| MS.gene85501.t1 | AT2G26390 | 49.495 | 396 | 178 | 6 | 1 | 384 | 1 | 386 | 1.94e-125 | 367 |
| MS.gene85501.t1 | AT1G64030 | 43.257 | 393 | 192 | 5 | 1 | 384 | 1 | 371 | 3.95e-104 | 313 |
| MS.gene85501.t1 | AT2G14540 | 43.878 | 392 | 189 | 6 | 1 | 384 | 29 | 397 | 1.31e-102 | 309 |
| MS.gene85501.t1 | AT1G62170 | 42.424 | 396 | 190 | 7 | 1 | 384 | 62 | 431 | 8.88e-102 | 308 |
| MS.gene85501.t1 | AT2G35580 | 43.257 | 393 | 178 | 9 | 1 | 384 | 15 | 371 | 3.58e-100 | 302 |
| MS.gene85501.t1 | AT1G62170 | 39.161 | 429 | 190 | 8 | 1 | 384 | 62 | 464 | 8.58e-95 | 291 |
| MS.gene85501.t1 | AT1G64010 | 45.205 | 219 | 92 | 5 | 170 | 384 | 1 | 195 | 1.99e-59 | 191 |
| MS.gene85501.t1 | AT1G64010 | 44.118 | 204 | 86 | 5 | 185 | 384 | 2 | 181 | 1.51e-51 | 171 |
| MS.gene85501.t1 | AT1G63280 | 56.780 | 118 | 47 | 2 | 126 | 241 | 5 | 120 | 9.68e-37 | 130 |
| MS.gene85501.t1 | AT1G62160 | 37.879 | 198 | 97 | 5 | 192 | 384 | 42 | 218 | 2.22e-34 | 127 |
| MS.gene85501.t1 | AT1G51330 | 50.000 | 120 | 50 | 1 | 103 | 222 | 15 | 124 | 2.15e-28 | 110 |
| MS.gene85501.t1 | AT1G64020 | 32.231 | 121 | 79 | 3 | 1 | 119 | 1 | 120 | 2.75e-14 | 69.3 |
Find 81 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAATGCACTCTACTTTAA+AGG | 0.141946 | 3.4:+12282723 | MS.gene85501:CDS |
| TGACTAAAGAAGTGAATTTA+TGG | 0.193244 | 3.4:+12282622 | MS.gene85501:CDS |
| TTATCTTTGCCTTTCTTATA+CGG | 0.278481 | 3.4:-12282891 | None:intergenic |
| GAGGAAGAAGTTCTTTAATA+AGG | 0.287252 | 3.4:-12282665 | None:intergenic |
| GTTGAGATGATCGGTGGATT+TGG | 0.313779 | 3.4:-12281778 | None:intergenic |
| GACAGTCCATCTTTAGCATT+TGG | 0.341529 | 3.4:-12282942 | None:intergenic |
| ACTGGAACCATTCTCTTTGT+TGG | 0.359618 | 3.4:+12283338 | MS.gene85501:CDS |
| TATTTCTGATTAGAGAAGAT+TGG | 0.360147 | 3.4:+12283315 | MS.gene85501:CDS |
| TCTCCTTCGTCAATGGTGTA+TGG | 0.363806 | 3.4:+12281865 | MS.gene85501:CDS |
| TTCTTTCAAGAACAGATGTT+TGG | 0.395078 | 3.4:-12281646 | None:intergenic |
| TCTCATATGTTGAAGGAGTT+AGG | 0.397032 | 3.4:+12283086 | MS.gene85501:CDS |
| TGAAGGAAGGTTGAAGAGAA+AGG | 0.398588 | 3.4:-12281899 | None:intergenic |
| TTTCTTCCAAATGCTAAAGA+TGG | 0.400337 | 3.4:+12282936 | MS.gene85501:CDS |
| CCTCGACTCTCCTTCGTCAA+TGG | 0.430388 | 3.4:+12281858 | MS.gene85501:CDS |
| AGATGACACGATTTCTTTGA+AGG | 0.432418 | 3.4:-12281916 | None:intergenic |
| TGCATAGGGAAACATTCGTT+TGG | 0.439694 | 3.4:-12281620 | None:intergenic |
| GTCAGCATTGGTTGATGAGT+TGG | 0.440539 | 3.4:+12282962 | MS.gene85501:CDS |
| GTTAAGCTATCAACTGATCT+TGG | 0.441723 | 3.4:-12282687 | None:intergenic |
| TGATACTTCTCATATGTTGA+AGG | 0.449488 | 3.4:+12283079 | MS.gene85501:CDS |
| CTCTCCGATGCTGCTCCCAC+AGG | 0.449677 | 3.4:+12281831 | MS.gene85501:CDS |
| GAAGGAAGGTTGAAGAGAAA+GGG | 0.450222 | 3.4:-12281898 | None:intergenic |
| GACTAAAGAAGTGAATTTAT+GGG | 0.454605 | 3.4:+12282623 | MS.gene85501:CDS |
| CTTCGTCTTCCGTATAAGAA+AGG | 0.461270 | 3.4:+12282882 | MS.gene85501:CDS |
| TTCATTGAGGTAAATGAAGA+AGG | 0.465817 | 3.4:+12283206 | MS.gene85501:CDS |
| CATCTCCTCGCTGGTCATGA+AGG | 0.480094 | 3.4:-12282826 | None:intergenic |
| TCCACCTGTGGGAGCAGCAT+CGG | 0.481475 | 3.4:-12281835 | None:intergenic |
| GTCAAACCTCCGGAAAAGAA+AGG | 0.482483 | 3.4:-12283119 | None:intergenic |
| TCTCTAATCAGAAATAAGAA+AGG | 0.483189 | 3.4:-12283308 | None:intergenic |
| TAGGGAAACATTCGTTTGGT+TGG | 0.488135 | 3.4:-12281616 | None:intergenic |
| AAGCTGAGTTTGAATACCCT+TGG | 0.490459 | 3.4:-12283267 | None:intergenic |
| AGACTATAGTGTAGTAAGTC+AGG | 0.490539 | 3.4:-12282993 | None:intergenic |
| CTCCTTCGTCAATGGTGTAT+GGG | 0.491333 | 3.4:+12281866 | MS.gene85501:CDS |
| TGCATTAGCAAAGATGAGTC+TGG | 0.499282 | 3.4:-12282709 | None:intergenic |
| CTGATTAGAGAAGATTGGAC+TGG | 0.501644 | 3.4:+12283320 | MS.gene85501:CDS |
| ATGGTGGACTCTTCTCTTGT+GGG | 0.507953 | 3.4:+12283146 | MS.gene85501:CDS |
| ATCATCGCTGCTGGCTCTGA+GGG | 0.511801 | 3.4:+12281720 | MS.gene85501:CDS |
| GTACTAAGCATCATCGCTGC+TGG | 0.512015 | 3.4:+12281711 | MS.gene85501:CDS |
| CACTCTACTTTAAAGGAGCA+TGG | 0.514338 | 3.4:+12282730 | MS.gene85501:CDS |
| TCCGATGCTGCTCCCACAGG+TGG | 0.515601 | 3.4:+12281834 | MS.gene85501:CDS |
| TGACCGAGCTGCCATTCAAA+AGG | 0.515686 | 3.4:-12282797 | None:intergenic |
| CTCTAATCAGAAATAAGAAA+GGG | 0.516757 | 3.4:-12283307 | None:intergenic |
| CATCATCGCTGCTGGCTCTG+AGG | 0.516897 | 3.4:+12281719 | MS.gene85501:CDS |
| CAGATGTTTGGCAATGCATA+GGG | 0.522319 | 3.4:-12281634 | None:intergenic |
| GACACGATTTCTTTGAAGGA+AGG | 0.523677 | 3.4:-12281912 | None:intergenic |
| TGCTGCAATATGTTGTTCCA+AGG | 0.525097 | 3.4:+12283250 | MS.gene85501:CDS |
| TCACCTACTTCTGCTTCAGC+AGG | 0.528408 | 3.4:-12283020 | None:intergenic |
| CACTTGTCCAACAAAGAGAA+TGG | 0.528533 | 3.4:-12283345 | None:intergenic |
| TTTGAATGGCAGCTCGGTCA+AGG | 0.529731 | 3.4:+12282800 | MS.gene85501:CDS |
| CGTCCTGCTGAAGCAGAAGT+AGG | 0.536267 | 3.4:+12283017 | MS.gene85501:CDS |
| ACAGATGTTTGGCAATGCAT+AGG | 0.540369 | 3.4:-12281635 | None:intergenic |
| AATGGTGGACTCTTCTCTTG+TGG | 0.543680 | 3.4:+12283145 | MS.gene85501:CDS |
| AATAAACTGCATCTCCTCGC+TGG | 0.546179 | 3.4:-12282835 | None:intergenic |
| ATCTCAACGATCTCACCTCT+CGG | 0.546457 | 3.4:+12281793 | MS.gene85501:CDS |
| ATTTCACAAATCTTTCATTG+AGG | 0.547073 | 3.4:+12283193 | MS.gene85501:CDS |
| GAAATATATTAGAAACAGAA+AGG | 0.558448 | 3.4:-12283175 | None:intergenic |
| GATGATCGGTGGATTTGGAC+TGG | 0.563091 | 3.4:-12281773 | None:intergenic |
| AAGCTATCAACTGATCTTGG+AGG | 0.569083 | 3.4:-12282684 | None:intergenic |
| GGTGAGATCGTTGAGATGAT+CGG | 0.570735 | 3.4:-12281787 | None:intergenic |
| ATCTCCTCGCTGGTCATGAA+GGG | 0.571071 | 3.4:-12282825 | None:intergenic |
| GCTGCAATATGTTGTTCCAA+GGG | 0.578130 | 3.4:+12283251 | MS.gene85501:CDS |
| TTATGTTGATTTCATAACCA+AGG | 0.579962 | 3.4:+12281959 | MS.gene85501:CDS |
| TGCGAAGATCACTCATCAAG+AGG | 0.581401 | 3.4:-12283374 | None:intergenic |
| GTCGAGAAGCTGTTGTTGTG+TGG | 0.586119 | 3.4:-12281745 | None:intergenic |
| TTTGGTTGGTGATTGATTCG+CGG | 0.591419 | 3.4:-12281602 | None:intergenic |
| GAAATCAACATAAGCCATTG+TGG | 0.594578 | 3.4:-12281949 | None:intergenic |
| TCGAGAAGCTGTTGTTGTGT+GGG | 0.605173 | 3.4:-12281744 | None:intergenic |
| TATGTTGAAGGAGTTAGGAG+TGG | 0.615125 | 3.4:+12283091 | MS.gene85501:CDS |
| ATCTAATTACGAGGCCACAA+TGG | 0.618182 | 3.4:+12281935 | MS.gene85501:CDS |
| GGGAGCAGCATCGGAGAGCA+CGG | 0.622379 | 3.4:-12281826 | None:intergenic |
| GAGAGTCGAGGTCCACCTGT+GGG | 0.635303 | 3.4:-12281846 | None:intergenic |
| TAAAGATGGACTGTCAGCAT+TGG | 0.637494 | 3.4:+12282950 | MS.gene85501:CDS |
| GATCGGTGGATTTGGACTGG+AGG | 0.638723 | 3.4:-12281770 | None:intergenic |
| TAATCAGAAATAAGAAAGGG+TGG | 0.640706 | 3.4:-12283304 | None:intergenic |
| GGAGAGTCGAGGTCCACCTG+TGG | 0.645915 | 3.4:-12281847 | None:intergenic |
| GAGATCGTTGAGATGATCGG+TGG | 0.659047 | 3.4:-12281784 | None:intergenic |
| AACCCATACACCATTGACGA+AGG | 0.669489 | 3.4:-12281868 | None:intergenic |
| AATCGTGTCATCTAATTACG+AGG | 0.678044 | 3.4:+12281926 | MS.gene85501:CDS |
| CACGGTAGAAACAAGCCGAG+AGG | 0.685748 | 3.4:-12281808 | None:intergenic |
| AGTACAACTTGAAGAGACAG+TGG | 0.716234 | 3.4:-12281693 | None:intergenic |
| CCATTGACGAAGGAGAGTCG+AGG | 0.741972 | 3.4:-12281858 | None:intergenic |
| GGTTCCCTTCATGACCAGCG+AGG | 0.762854 | 3.4:+12282821 | MS.gene85501:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATTTTATATGGTTTTAA+AGG | + | chr3.4:12282590-12282609 | MS.gene85501:intron | 10.0% |
| !!! | ATTTAAAAATCAATTAGCTT+TGG | - | chr3.4:12282575-12282594 | None:intergenic | 15.0% |
| !! | ATAAAATAAACTGTTTGAGA+AGG | - | chr3.4:12282055-12282074 | None:intergenic | 20.0% |
| !! | GAAATATATTAGAAACAGAA+AGG | - | chr3.4:12283178-12283197 | None:intergenic | 20.0% |
| !!! | GATTTTCCATTAACAATTAA+TGG | + | chr3.4:12282024-12282043 | MS.gene85501:intron | 20.0% |
| ! | ACTTATAAGTATAATGCTCT+TGG | + | chr3.4:12282152-12282171 | MS.gene85501:intron | 25.0% |
| ! | ATTTCACAAATCTTTCATTG+AGG | + | chr3.4:12283193-12283212 | MS.gene85501:CDS | 25.0% |
| ! | CAATTAGTATTTAGAAGCTT+TGG | - | chr3.4:12282441-12282460 | None:intergenic | 25.0% |
| ! | CATCATAACTATATGATTGA+AGG | - | chr3.4:12282275-12282294 | None:intergenic | 25.0% |
| ! | CTCTAATCAGAAATAAGAAA+GGG | - | chr3.4:12283310-12283329 | None:intergenic | 25.0% |
| ! | GACTAAAGAAGTGAATTTAT+GGG | + | chr3.4:12282623-12282642 | MS.gene85501:CDS | 25.0% |
| ! | GTAGATCCATTAATTGTTAA+TGG | - | chr3.4:12282033-12282052 | None:intergenic | 25.0% |
| ! | TCATATAGTTATGATGCATT+TGG | + | chr3.4:12282279-12282298 | MS.gene85501:intron | 25.0% |
| ! | TCTCTAATCAGAAATAAGAA+AGG | - | chr3.4:12283311-12283330 | None:intergenic | 25.0% |
| ! | TGACTAAAGAAGTGAATTTA+TGG | + | chr3.4:12282622-12282641 | MS.gene85501:CDS | 25.0% |
| ! | TTATCTTTGCCTTTCTTATA+CGG | - | chr3.4:12282894-12282913 | None:intergenic | 25.0% |
| !! | AAAGTAGGTTCTTCTTTTAA+AGG | + | chr3.4:12282208-12282227 | MS.gene85501:intron | 25.0% |
| !! | ATTGGTGGTATATACTATAA+TGG | + | chr3.4:12282302-12282321 | MS.gene85501:intron | 25.0% |
| !! | TATGATTTTCACCTTTTGAA+TGG | + | chr3.4:12282786-12282805 | MS.gene85501:CDS | 25.0% |
| !! | TATTTCTGATTAGAGAAGAT+TGG | + | chr3.4:12283315-12283334 | MS.gene85501:CDS | 25.0% |
| !! | TCATACGAAATTTTGAATCT+TGG | - | chr3.4:12283056-12283075 | None:intergenic | 25.0% |
| !!! | TTATGTTGATTTCATAACCA+AGG | + | chr3.4:12281959-12281978 | MS.gene85501:CDS | 25.0% |
| ATACCTCCAAATATACAAGA+TGG | - | chr3.4:12282505-12282524 | None:intergenic | 30.0% | |
| GAGGAAGAAGTTCTTTAATA+AGG | - | chr3.4:12282668-12282687 | None:intergenic | 30.0% | |
| GTATGTCCATCTTGTATATT+TGG | + | chr3.4:12282496-12282515 | MS.gene85501:intron | 30.0% | |
| GTTAATGGAAAATCAAAGCA+GGG | - | chr3.4:12282018-12282037 | None:intergenic | 30.0% | |
| GTTTATTTCAGCACAAAGTA+GGG | + | chr3.4:12282460-12282479 | MS.gene85501:intron | 30.0% | |
| TAATCAGAAATAAGAAAGGG+TGG | - | chr3.4:12283307-12283326 | None:intergenic | 30.0% | |
| TAGTTATGATGCATTTGGAT+TGG | + | chr3.4:12282284-12282303 | MS.gene85501:intron | 30.0% | |
| TGATACTTCTCATATGTTGA+AGG | + | chr3.4:12283079-12283098 | MS.gene85501:CDS | 30.0% | |
| TGCTCTTGGAATTTATTTGA+CGG | + | chr3.4:12282166-12282185 | MS.gene85501:intron | 30.0% | |
| TGTTAATGGAAAATCAAAGC+AGG | - | chr3.4:12282019-12282038 | None:intergenic | 30.0% | |
| TGTTTATTTCAGCACAAAGT+AGG | + | chr3.4:12282459-12282478 | MS.gene85501:intron | 30.0% | |
| TTCATTGAGGTAAATGAAGA+AGG | + | chr3.4:12283206-12283225 | MS.gene85501:CDS | 30.0% | |
| TTCTTTCAAGAACAGATGTT+TGG | - | chr3.4:12281649-12281668 | None:intergenic | 30.0% | |
| TTGATTCTAGTGTAGAAAGT+AGG | + | chr3.4:12282193-12282212 | MS.gene85501:intron | 30.0% | |
| TTTCTTCCAAATGCTAAAGA+TGG | + | chr3.4:12282936-12282955 | MS.gene85501:CDS | 30.0% | |
| ! | ATAAAAGGGTATACTTGGAT+TGG | - | chr3.4:12282393-12282412 | None:intergenic | 30.0% |
| ! | ATCCAAGTATACCCTTTTAT+TGG | + | chr3.4:12282393-12282412 | MS.gene85501:intron | 30.0% |
| ! | TGGTATATACTATAATGGAG+TGG | + | chr3.4:12282307-12282326 | MS.gene85501:intron | 30.0% |
| !! | GATTGGTGTTATTGAGTAAA+AGG | - | chr3.4:12282376-12282395 | None:intergenic | 30.0% |
| AATCGTGTCATCTAATTACG+AGG | + | chr3.4:12281926-12281945 | MS.gene85501:CDS | 35.0% | |
| ACATCCAACTAGCCAATAAA+AGG | - | chr3.4:12282408-12282427 | None:intergenic | 35.0% | |
| AGACTATAGTGTAGTAAGTC+AGG | - | chr3.4:12282996-12283015 | None:intergenic | 35.0% | |
| AGATGACACGATTTCTTTGA+AGG | - | chr3.4:12281919-12281938 | None:intergenic | 35.0% | |
| CATCCAACTAGCCAATAAAA+GGG | - | chr3.4:12282407-12282426 | None:intergenic | 35.0% | |
| GAAATCAACATAAGCCATTG+TGG | - | chr3.4:12281952-12281971 | None:intergenic | 35.0% | |
| GCTAATGCACTCTACTTTAA+AGG | + | chr3.4:12282723-12282742 | MS.gene85501:CDS | 35.0% | |
| GGAGTGGAATGAAATGATTT+GGG | + | chr3.4:12282323-12282342 | MS.gene85501:intron | 35.0% | |
| GTTAAGCTATCAACTGATCT+TGG | - | chr3.4:12282690-12282709 | None:intergenic | 35.0% | |
| TCTCATATGTTGAAGGAGTT+AGG | + | chr3.4:12283086-12283105 | MS.gene85501:CDS | 35.0% | |
| TGGAGTGGAATGAAATGATT+TGG | + | chr3.4:12282322-12282341 | MS.gene85501:intron | 35.0% | |
| TGTCCATCTTGTATATTTGG+AGG | + | chr3.4:12282499-12282518 | MS.gene85501:intron | 35.0% | |
| ! | AGCCAATAAAAGGGTATACT+TGG | - | chr3.4:12282398-12282417 | None:intergenic | 35.0% |
| ! | TTATGATGCATTTGGATTGG+TGG | + | chr3.4:12282287-12282306 | MS.gene85501:intron | 35.0% |
| !! | ATACCCTTTTATTGGCTAGT+TGG | + | chr3.4:12282401-12282420 | MS.gene85501:intron | 35.0% |
| !! | GTGGTTTTACCTTTCTTTTC+CGG | + | chr3.4:12283110-12283129 | MS.gene85501:CDS | 35.0% |
| AAGCTATCAACTGATCTTGG+AGG | - | chr3.4:12282687-12282706 | None:intergenic | 40.0% | |
| AAGCTGAGTTTGAATACCCT+TGG | - | chr3.4:12283270-12283289 | None:intergenic | 40.0% | |
| ACAGATGTTTGGCAATGCAT+AGG | - | chr3.4:12281638-12281657 | None:intergenic | 40.0% | |
| ACTGGAACCATTCTCTTTGT+TGG | + | chr3.4:12283338-12283357 | MS.gene85501:CDS | 40.0% | |
| AGGATGAAGTGTGTGATTCA+TGG | + | chr3.4:12282228-12282247 | MS.gene85501:intron | 40.0% | |
| AGTACAACTTGAAGAGACAG+TGG | - | chr3.4:12281696-12281715 | None:intergenic | 40.0% | |
| ATCTAATTACGAGGCCACAA+TGG | + | chr3.4:12281935-12281954 | MS.gene85501:CDS | 40.0% | |
| CACTTGTCCAACAAAGAGAA+TGG | - | chr3.4:12283348-12283367 | None:intergenic | 40.0% | |
| CAGATGTTTGGCAATGCATA+GGG | - | chr3.4:12281637-12281656 | None:intergenic | 40.0% | |
| CTGATTAGAGAAGATTGGAC+TGG | + | chr3.4:12283320-12283339 | MS.gene85501:CDS | 40.0% | |
| CTTCGTCTTCCGTATAAGAA+AGG | + | chr3.4:12282882-12282901 | MS.gene85501:CDS | 40.0% | |
| GAAGGAAGGTTGAAGAGAAA+GGG | - | chr3.4:12281901-12281920 | None:intergenic | 40.0% | |
| GACAGTCCATCTTTAGCATT+TGG | - | chr3.4:12282945-12282964 | None:intergenic | 40.0% | |
| GCTGCAATATGTTGTTCCAA+GGG | + | chr3.4:12283251-12283270 | MS.gene85501:CDS | 40.0% | |
| TAGGGAAACATTCGTTTGGT+TGG | - | chr3.4:12281619-12281638 | None:intergenic | 40.0% | |
| TATGTTGAAGGAGTTAGGAG+TGG | + | chr3.4:12283091-12283110 | MS.gene85501:CDS | 40.0% | |
| TGAAGGAAGGTTGAAGAGAA+AGG | - | chr3.4:12281902-12281921 | None:intergenic | 40.0% | |
| TGCATAGGGAAACATTCGTT+TGG | - | chr3.4:12281623-12281642 | None:intergenic | 40.0% | |
| TGCATTAGCAAAGATGAGTC+TGG | - | chr3.4:12282712-12282731 | None:intergenic | 40.0% | |
| TGCTGCAATATGTTGTTCCA+AGG | + | chr3.4:12283250-12283269 | MS.gene85501:CDS | 40.0% | |
| TTCCGGAGGTTTGACAAAAA+TGG | + | chr3.4:12283127-12283146 | MS.gene85501:CDS | 40.0% | |
| ! | CACTCTACTTTAAAGGAGCA+TGG | + | chr3.4:12282730-12282749 | MS.gene85501:CDS | 40.0% |
| ! | GACACGATTTCTTTGAAGGA+AGG | - | chr3.4:12281915-12281934 | None:intergenic | 40.0% |
| ! | GTTTTACCTTTCTTTTCCGG+AGG | + | chr3.4:12283113-12283132 | MS.gene85501:CDS | 40.0% |
| !! | CACCATTTTTGTCAAACCTC+CGG | - | chr3.4:12283132-12283151 | None:intergenic | 40.0% |
| !! | TAAAGATGGACTGTCAGCAT+TGG | + | chr3.4:12282950-12282969 | MS.gene85501:CDS | 40.0% |
| !! | TTTGGTTGGTGATTGATTCG+CGG | - | chr3.4:12281605-12281624 | None:intergenic | 40.0% |
| AACCCATACACCATTGACGA+AGG | - | chr3.4:12281871-12281890 | None:intergenic | 45.0% | |
| AATAAACTGCATCTCCTCGC+TGG | - | chr3.4:12282838-12282857 | None:intergenic | 45.0% | |
| AGCAGGGCATGAATTGAAAG+AGG | - | chr3.4:12282002-12282021 | None:intergenic | 45.0% | |
| AGGGCATGAATTGAAAGAGG+GGG | - | chr3.4:12281999-12282018 | None:intergenic | 45.0% | |
| ATCTCAACGATCTCACCTCT+CGG | + | chr3.4:12281793-12281812 | MS.gene85501:CDS | 45.0% | |
| CAGGGCATGAATTGAAAGAG+GGG | - | chr3.4:12282000-12282019 | None:intergenic | 45.0% | |
| CGGAGGTTTGACAAAAATGG+TGG | + | chr3.4:12283130-12283149 | MS.gene85501:CDS | 45.0% | |
| CTCCTTCGTCAATGGTGTAT+GGG | + | chr3.4:12281866-12281885 | MS.gene85501:CDS | 45.0% | |
| GCAGGGCATGAATTGAAAGA+GGG | - | chr3.4:12282001-12282020 | None:intergenic | 45.0% | |
| GGTGAGATCGTTGAGATGAT+CGG | - | chr3.4:12281790-12281809 | None:intergenic | 45.0% | |
| GTCAAACCTCCGGAAAAGAA+AGG | - | chr3.4:12283122-12283141 | None:intergenic | 45.0% | |
| TCGAGAAGCTGTTGTTGTGT+GGG | - | chr3.4:12281747-12281766 | None:intergenic | 45.0% | |
| TCTCCTTCGTCAATGGTGTA+TGG | + | chr3.4:12281865-12281884 | MS.gene85501:CDS | 45.0% | |
| TGGGCTGAAAAAGAGACGAA+TGG | + | chr3.4:12282642-12282661 | MS.gene85501:CDS | 45.0% | |
| ! | GGGGGTATACAAACATACCT+TGG | - | chr3.4:12281979-12281998 | None:intergenic | 45.0% |
| ! | GTTGAGATGATCGGTGGATT+TGG | - | chr3.4:12281781-12281800 | None:intergenic | 45.0% |
| ! | TCACCTTTTGAATGGCAGCT+CGG | + | chr3.4:12282794-12282813 | MS.gene85501:CDS | 45.0% |
| !! | AATGGTGGACTCTTCTCTTG+TGG | + | chr3.4:12283145-12283164 | MS.gene85501:CDS | 45.0% |
| !! | ATGGTGGACTCTTCTCTTGT+GGG | + | chr3.4:12283146-12283165 | MS.gene85501:CDS | 45.0% |
| !! | GTCAGCATTGGTTGATGAGT+TGG | + | chr3.4:12282962-12282981 | MS.gene85501:CDS | 45.0% |
| !!! | ATTGATTTTTAAATTTTATA+TGG | + | chr3.4:12282581-12282600 | MS.gene85501:intron | 5.0% |
| ATCTCCTCGCTGGTCATGAA+GGG | - | chr3.4:12282828-12282847 | None:intergenic | 50.0% | |
| GAGATCGTTGAGATGATCGG+TGG | - | chr3.4:12281787-12281806 | None:intergenic | 50.0% | |
| GGCATGAATTGAAAGAGGGG+GGG | - | chr3.4:12281997-12282016 | None:intergenic | 50.0% | |
| GGGCATGAATTGAAAGAGGG+GGG | - | chr3.4:12281998-12282017 | None:intergenic | 50.0% | |
| GTACTAAGCATCATCGCTGC+TGG | + | chr3.4:12281711-12281730 | MS.gene85501:CDS | 50.0% | |
| GTCGAGAAGCTGTTGTTGTG+TGG | - | chr3.4:12281748-12281767 | None:intergenic | 50.0% | |
| TCACCTACTTCTGCTTCAGC+AGG | - | chr3.4:12283023-12283042 | None:intergenic | 50.0% | |
| TGACCGAGCTGCCATTCAAA+AGG | - | chr3.4:12282800-12282819 | None:intergenic | 50.0% | |
| TTTGAATGGCAGCTCGGTCA+AGG | + | chr3.4:12282800-12282819 | MS.gene85501:CDS | 50.0% | |
| ! | GATGATCGGTGGATTTGGAC+TGG | - | chr3.4:12281776-12281795 | None:intergenic | 50.0% |
| CACGGTAGAAACAAGCCGAG+AGG | - | chr3.4:12281811-12281830 | None:intergenic | 55.0% | |
| CATCTCCTCGCTGGTCATGA+AGG | - | chr3.4:12282829-12282848 | None:intergenic | 55.0% | |
| CCATTGACGAAGGAGAGTCG+AGG | - | chr3.4:12281861-12281880 | None:intergenic | 55.0% | |
| CCTCGACTCTCCTTCGTCAA+TGG | + | chr3.4:12281858-12281877 | MS.gene85501:CDS | 55.0% | |
| CGTCCTGCTGAAGCAGAAGT+AGG | + | chr3.4:12283017-12283036 | MS.gene85501:CDS | 55.0% | |
| ! | GATCGGTGGATTTGGACTGG+AGG | - | chr3.4:12281773-12281792 | None:intergenic | 55.0% |
| !! | ATCATCGCTGCTGGCTCTGA+GGG | + | chr3.4:12281720-12281739 | MS.gene85501:CDS | 55.0% |
| GAGAGTCGAGGTCCACCTGT+GGG | - | chr3.4:12281849-12281868 | None:intergenic | 60.0% | |
| GGTTCCCTTCATGACCAGCG+AGG | + | chr3.4:12282821-12282840 | MS.gene85501:CDS | 60.0% | |
| TCCACCTGTGGGAGCAGCAT+CGG | - | chr3.4:12281838-12281857 | None:intergenic | 60.0% | |
| !! | CATCATCGCTGCTGGCTCTG+AGG | + | chr3.4:12281719-12281738 | MS.gene85501:CDS | 60.0% |
| CTCTCCGATGCTGCTCCCAC+AGG | + | chr3.4:12281831-12281850 | MS.gene85501:CDS | 65.0% | |
| GGAGAGTCGAGGTCCACCTG+TGG | - | chr3.4:12281850-12281869 | None:intergenic | 65.0% | |
| TCCGATGCTGCTCCCACAGG+TGG | + | chr3.4:12281834-12281853 | MS.gene85501:CDS | 65.0% | |
| ! | GGGAGCAGCATCGGAGAGCA+CGG | - | chr3.4:12281829-12281848 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 12281594 | 12283388 | 12281594 | ID=MS.gene85501 |
| chr3.4 | mRNA | 12281594 | 12283388 | 12281594 | ID=MS.gene85501.t1;Parent=MS.gene85501 |
| chr3.4 | exon | 12281594 | 12281980 | 12281594 | ID=MS.gene85501.t1.exon1;Parent=MS.gene85501.t1 |
| chr3.4 | CDS | 12281594 | 12281980 | 12281594 | ID=cds.MS.gene85501.t1;Parent=MS.gene85501.t1 |
| chr3.4 | exon | 12282612 | 12283388 | 12282612 | ID=MS.gene85501.t1.exon2;Parent=MS.gene85501.t1 |
| chr3.4 | CDS | 12282612 | 12283388 | 12282612 | ID=cds.MS.gene85501.t1;Parent=MS.gene85501.t1 |
| Gene Sequence |
| Protein sequence |
