Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85693.t1 | XP_028762943.1 | 63.8 | 94 | 28 | 1 | 12 | 99 | 255 | 348 | 2.00E-26 | 128.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85693.t1 | A0A392P9H0 | 64.4 | 90 | 31 | 1 | 2 | 91 | 107 | 195 | 4.7e-25 | 123.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056595 | MS.gene85693 | 0.810642 | 1.04E-50 | -1.69E-46 |
| MS.gene056854 | MS.gene85693 | 0.824469 | 7.94E-54 | -1.69E-46 |
| MS.gene059272 | MS.gene85693 | 0.826271 | 2.97E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85693.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 19 | 99 | 5 | 80 | 2.63e-25 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 6.1:-17475535 | MS.gene85693:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 6.1:-17475543 | MS.gene85693:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 6.1:+17475381 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 6.1:+17475566 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 6.1:-17475483 | MS.gene85693:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 6.1:-17475491 | MS.gene85693:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 6.1:-17475614 | MS.gene85693:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 6.1:+17475453 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 6.1:-17475665 | MS.gene85693:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 6.1:-17475534 | MS.gene85693:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 6.1:-17475607 | MS.gene85693:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 6.1:-17475399 | MS.gene85693:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 6.1:-17475623 | MS.gene85693:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 6.1:-17475620 | MS.gene85693:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 6.1:+17475454 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr6.1:17475400-17475419 | MS.gene85693:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr6.1:17475531-17475550 | MS.gene85693:CDS | 20.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr6.1:17475530-17475549 | MS.gene85693:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr6.1:17475574-17475593 | MS.gene85693:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr6.1:17475582-17475601 | MS.gene85693:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr6.1:17475502-17475521 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr6.1:17475663-17475682 | None:intergenic | 25.0% |
| CATTAGAGATGAAAATATCC+AGG | - | chr6.1:17475666-17475685 | MS.gene85693:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr6.1:17475522-17475541 | MS.gene85693:CDS | 30.0% | |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr6.1:17475525-17475544 | None:intergenic | 30.0% |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr6.1:17475646-17475665 | None:intergenic | 35.0% |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr6.1:17475615-17475634 | None:intergenic | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr6.1:17475614-17475633 | None:intergenic | 40.0% | |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr6.1:17475445-17475464 | MS.gene85693:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr6.1:17475442-17475461 | MS.gene85693:CDS | 40.0% |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr6.1:17475458-17475477 | MS.gene85693:CDS | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr6.1:17475451-17475470 | MS.gene85693:CDS | 45.0% | |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr6.1:17475491-17475510 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 17475394 | 17475693 | 17475394 | ID=MS.gene85693 |
| chr6.1 | mRNA | 17475394 | 17475693 | 17475394 | ID=MS.gene85693.t1;Parent=MS.gene85693 |
| chr6.1 | exon | 17475394 | 17475693 | 17475394 | ID=MS.gene85693.t1.exon1;Parent=MS.gene85693.t1 |
| chr6.1 | CDS | 17475394 | 17475693 | 17475394 | ID=cds.MS.gene85693.t1;Parent=MS.gene85693.t1 |
| Gene Sequence |
| Protein sequence |
