AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85933.t1 MTR_2g103620 77.427 412 48 6 23 430 8 378 0.0 541
MS.gene85933.t1 MTR_2g103640 76.781 379 56 7 27 400 2 353 1.65e-176 501
MS.gene85933.t1 MTR_5g038810 45.010 511 161 9 8 431 6 483 2.40e-110 343
MS.gene85933.t1 MTR_2g091090 43.725 510 170 8 24 425 39 539 7.49e-99 313
MS.gene85933.t1 MTR_2g091015 53.867 375 111 8 115 431 499 869 1.08e-95 310
MS.gene85933.t1 MTR_2g091015 35.061 328 134 9 22 284 51 364 1.85e-27 117
MS.gene85933.t1 MTR_2g091015 36.207 174 91 2 114 270 449 619 4.40e-15 79.0
MS.gene85933.t1 MTR_2g091015 40.667 150 75 3 127 262 304 453 3.49e-14 76.3
MS.gene85933.t1 MTR_2g091015 32.068 237 85 6 133 295 370 604 4.99e-14 75.5
MS.gene85933.t1 MTR_2g091105 42.011 557 167 11 21 430 37 584 2.03e-92 297
MS.gene85933.t1 MTR_2g091030 44.444 531 155 12 23 430 75 588 4.77e-92 293
MS.gene85933.t1 MTR_2g091285 41.328 542 174 12 23 430 58 589 2.75e-87 281
MS.gene85933.t1 MTR_2g091335 54.469 358 120 8 109 430 254 604 1.24e-83 272
MS.gene85933.t1 MTR_2g091335 37.049 305 123 11 23 277 57 342 3.58e-33 134
MS.gene85933.t1 MTR_5g078510 44.310 413 164 7 80 431 36 443 9.70e-79 257
MS.gene85933.t1 MTR_5g078510 33.516 182 76 3 136 272 1 182 4.27e-14 75.5
MS.gene85933.t1 MTR_6g045183 45.202 396 141 8 117 439 284 676 1.12e-78 262
MS.gene85933.t1 MTR_6g045183 33.117 308 130 6 17 270 75 360 5.76e-25 109
MS.gene85933.t1 MTR_2g091240 42.489 466 192 11 35 439 49 499 1.74e-77 255
MS.gene85933.t1 MTR_2g093010 49.041 365 159 9 82 431 467 819 8.93e-77 259
MS.gene85933.t1 MTR_2g093010 41.045 268 112 10 45 274 64 323 1.20e-28 121
MS.gene85933.t1 MTR_2g093010 30.952 252 139 5 40 270 208 445 3.76e-14 75.9
MS.gene85933.t1 MTR_2g093010 49.041 365 159 9 82 431 468 820 1.15e-76 259
MS.gene85933.t1 MTR_2g093010 41.045 268 112 10 45 274 65 324 1.35e-28 121
MS.gene85933.t1 MTR_2g093010 30.952 252 139 5 40 270 209 446 4.07e-14 75.9
MS.gene85933.t1 MTR_2g091245 49.702 336 120 6 128 431 241 559 1.31e-72 244
MS.gene85933.t1 MTR_2g091250 47.396 384 151 7 90 430 816 1191 3.33e-70 244
MS.gene85933.t1 MTR_2g091250 38.438 320 118 7 24 270 52 365 1.65e-41 160
MS.gene85933.t1 MTR_2g091250 40.110 182 88 6 103 270 258 432 5.26e-18 88.6
MS.gene85933.t1 MTR_2g091250 36.649 191 86 3 115 270 480 670 3.79e-17 85.9
MS.gene85933.t1 MTR_2g091250 39.130 184 89 4 108 270 391 572 1.70e-16 83.6
MS.gene85933.t1 MTR_2g091250 39.355 155 77 2 133 270 656 810 1.94e-16 83.6
MS.gene85933.t1 MTR_2g091250 36.879 141 86 1 133 270 586 726 5.60e-16 82.0
MS.gene85933.t1 MTR_2g091250 32.569 218 111 4 112 293 365 582 8.21e-15 78.2
MS.gene85933.t1 MTR_2g091250 34.010 197 68 4 133 270 337 530 4.27e-14 75.9
MS.gene85933.t1 MTR_2g091250 31.858 226 138 4 55 268 643 864 3.93e-12 69.7
MS.gene85933.t1 MTR_2g104190 49.835 303 116 5 136 429 332 607 2.13e-68 232
MS.gene85933.t1 MTR_2g104190 29.747 316 160 6 4 270 55 357 1.15e-17 86.7
MS.gene85933.t1 MTR_2g091025 37.323 493 200 11 22 447 82 532 2.99e-67 228
MS.gene85933.t1 MTR_2g091260 49.852 337 121 6 133 431 448 774 1.46e-65 229
MS.gene85933.t1 MTR_2g091260 36.306 314 143 8 20 286 78 381 1.62e-35 142
MS.gene85933.t1 MTR_2g091260 38.406 138 74 1 133 270 280 406 1.44e-13 73.9
MS.gene85933.t1 MTR_2g091260 37.748 151 91 1 133 280 336 486 1.04e-12 71.2
MS.gene85933.t1 MTR_2g010420 42.775 346 147 7 129 430 399 737 3.94e-65 225
MS.gene85933.t1 MTR_2g010420 31.122 196 111 4 99 270 278 473 1.06e-12 71.2
MS.gene85933.t1 MTR_2g010420 42.651 347 148 7 129 431 399 738 1.13e-64 226
MS.gene85933.t1 MTR_2g010420 31.122 196 111 4 99 270 278 473 1.28e-12 71.2
MS.gene85933.t1 MTR_2g091080 45.226 398 159 8 82 430 371 758 1.00e-62 219
MS.gene85933.t1 MTR_2g091080 38.148 270 124 12 27 271 29 280 3.67e-23 104
MS.gene85933.t1 MTR_2g091080 35.749 207 113 5 75 270 222 419 2.17e-17 86.3
MS.gene85933.t1 MTR_2g091290 39.192 421 207 9 57 434 324 738 4.39e-61 216
MS.gene85933.t1 MTR_2g091290 36.330 267 130 6 27 271 57 305 5.09e-26 113
MS.gene85933.t1 MTR_2g091290 33.898 177 64 3 133 267 276 441 8.41e-13 71.6
MS.gene85933.t1 MTR_3g110385 43.784 370 173 7 81 431 377 730 4.49e-61 216
MS.gene85933.t1 MTR_3g110385 44.853 136 71 1 135 270 211 342 9.57e-19 90.5
MS.gene85933.t1 MTR_3g110385 35.359 181 99 3 133 295 300 480 9.90e-15 77.8
MS.gene85933.t1 MTR_2g091370 38.402 388 164 7 129 447 372 753 4.88e-58 207
MS.gene85933.t1 MTR_2g091370 37.755 294 134 12 12 271 57 335 4.93e-24 107
MS.gene85933.t1 MTR_2g091370 27.341 267 146 6 49 267 205 471 1.38e-11 67.8
MS.gene85933.t1 MTR_2g091040 35.234 491 185 11 24 392 39 518 2.60e-55 194
MS.gene85933.t1 MTR_2g091110 40.088 454 163 12 82 430 749 1198 4.95e-55 200
MS.gene85933.t1 MTR_2g091110 37.915 211 103 5 81 270 650 853 7.79e-23 103
MS.gene85933.t1 MTR_2g091110 41.935 155 66 3 133 270 468 615 3.75e-18 89.0
MS.gene85933.t1 MTR_2g091110 32.166 314 146 11 2 262 476 775 5.80e-18 88.2
MS.gene85933.t1 MTR_2g091110 37.438 203 106 6 82 270 462 657 2.52e-15 80.1
MS.gene85933.t1 MTR_2g091110 38.415 164 89 3 115 270 365 524 6.97e-15 78.6
MS.gene85933.t1 MTR_2g091110 50.538 93 36 3 44 136 43 125 1.41e-13 74.3
MS.gene85933.t1 MTR_2g091110 39.189 148 65 3 130 270 556 685 7.27e-12 68.9
MS.gene85933.t1 MTR_2g090980 54.460 213 81 3 232 431 308 517 9.28e-54 191
MS.gene85933.t1 MTR_8g107320 54.315 197 73 4 222 417 24 204 2.29e-50 172
MS.gene85933.t1 MTR_8g106540 40.625 320 121 11 112 430 33 284 3.41e-44 158
MS.gene85933.t1 MTR_5g078500 38.346 266 119 5 24 251 40 298 3.37e-37 139
MS.gene85933.t1 MTR_2g091325 36.111 324 122 9 27 270 82 400 1.08e-34 138
MS.gene85933.t1 MTR_2g091325 51.087 184 62 5 115 270 364 547 4.80e-32 130
MS.gene85933.t1 MTR_2g091325 36.283 226 112 6 82 288 296 508 6.99e-21 96.7
MS.gene85933.t1 MTR_2g091325 38.514 148 74 3 133 270 288 428 2.24e-12 70.1
MS.gene85933.t1 MTR_2g091070 54.054 148 65 1 300 447 21 165 1.90e-33 126
MS.gene85933.t1 MTR_7g056313 35.220 318 131 8 22 283 35 333 2.16e-32 128
MS.gene85933.t1 MTR_7g056313 35.484 155 83 2 133 270 214 368 3.85e-13 71.6
MS.gene85933.t1 MTR_7g056313 37.500 168 76 4 115 257 206 369 6.37e-13 70.9
MS.gene85933.t1 MTR_2g091330 37.013 308 137 8 26 286 79 376 3.75e-28 119
MS.gene85933.t1 MTR_2g091330 39.877 163 74 3 135 273 431 593 6.39e-16 81.3
MS.gene85933.t1 MTR_2g091330 40.123 162 82 2 111 268 319 469 1.34e-12 70.9
MS.gene85933.t1 MTR_2g091330 40.171 117 66 1 112 224 467 583 1.56e-11 67.4
MS.gene85933.t1 MTR_8g099800 70.732 82 22 2 97 178 8 87 2.65e-27 106
MS.gene85933.t1 MTR_2g091010 36.066 305 146 12 2 272 51 340 1.58e-26 114
MS.gene85933.t1 MTR_2g091010 40.957 188 74 5 115 276 316 492 2.52e-19 92.0
MS.gene85933.t1 MTR_2g091010 37.200 250 112 5 81 292 373 615 3.47e-19 91.7
MS.gene85933.t1 MTR_2g104430 63.542 96 30 4 441 536 59 149 1.41e-25 110
MS.gene85933.t1 MTR_8g467200 35.052 291 165 7 152 434 32 306 1.25e-24 107
MS.gene85933.t1 MTR_2g104400 61.458 96 32 4 441 536 18 108 1.06e-23 106
MS.gene85933.t1 MTR_2g091280 41.989 181 60 2 135 270 531 711 1.71e-23 105
MS.gene85933.t1 MTR_2g091280 36.721 305 118 10 23 270 59 345 7.13e-22 100
MS.gene85933.t1 MTR_2g091280 41.722 151 81 2 127 270 307 457 1.84e-17 86.3
MS.gene85933.t1 MTR_2g091280 35.443 237 95 6 92 270 321 557 4.33e-16 82.0
MS.gene85933.t1 MTR_2g091280 33.488 215 91 5 115 277 407 621 8.90e-12 68.2
MS.gene85933.t1 MTR_2g095920 60.417 96 33 4 441 536 18 108 9.31e-23 102
MS.gene85933.t1 MTR_2g104430 59.375 96 28 5 441 536 18 102 3.32e-21 97.4
MS.gene85933.t1 MTR_2g104430 57.292 96 26 4 441 536 59 139 3.64e-21 97.4
MS.gene85933.t1 MTR_4g084920 33.562 292 149 10 106 362 10 291 2.13e-20 92.4
MS.gene85933.t1 MTR_4g046660 47.518 141 65 3 296 434 447 580 5.59e-20 94.4
MS.gene85933.t1 MTR_2g091315 33.333 282 132 9 27 270 49 312 2.41e-18 88.6
MS.gene85933.t1 MTR_2g091315 29.771 262 129 4 40 270 201 438 7.61e-13 71.2
MS.gene85933.t1 MTR_2g091360 52.174 92 44 0 133 224 65 156 6.02e-18 85.5
MS.gene85933.t1 MTR_4g009710 47.778 90 44 3 446 535 21 107 2.12e-17 83.6
MS.gene85933.t1 MTR_4g009710 49.254 67 33 1 446 512 140 205 3.69e-12 67.8
MS.gene85933.t1 MTR_2g091005 31.970 269 131 8 27 267 59 303 2.14e-17 85.9
MS.gene85933.t1 MTR_2g090640 41.259 143 73 1 133 275 197 328 4.27e-17 84.7
MS.gene85933.t1 MTR_2g090640 38.462 169 77 4 117 269 261 418 4.62e-13 72.0
MS.gene85933.t1 MTR_4g009740 51.648 91 41 3 446 536 21 108 8.90e-17 84.7
MS.gene85933.t1 MTR_7g056317 67.045 88 25 2 344 431 1 84 5.95e-16 74.3
MS.gene85933.t1 MTR_2g104400 58.108 74 28 3 463 536 1 71 7.78e-15 78.2
MS.gene85933.t1 MTR_4g009630 47.368 95 45 4 441 535 212 301 1.79e-14 75.5
MS.gene85933.t1 MTR_4g009630 47.368 95 45 4 441 535 212 301 2.28e-14 75.5
MS.gene85933.t1 MTR_2g064120 56.164 73 31 1 52 123 133 205 5.71e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85933.t1 AT1G15780 41.379 116 50 4 434 532 5 119 3.58e-15 79.7
MS.gene85933.t1 AT1G15780 41.379 116 50 4 434 532 27 141 3.66e-15 79.3
MS.gene85933.t1 AT1G15780 41.379 116 50 4 434 532 5 119 3.73e-15 79.3
MS.gene85933.t1 AT5G27220 30.448 335 192 13 99 425 497 798 9.00e-14 75.1
MS.gene85933.t1 AT5G27220 30.448 335 192 13 99 425 497 798 9.00e-14 75.1
MS.gene85933.t1 AT5G27220 30.448 335 192 13 99 425 497 798 9.01e-14 75.1
MS.gene85933.t1 AT5G27220 30.448 335 192 13 99 425 497 798 9.01e-14 75.1

Find 95 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCAATATTTCTCTTTA+TGG 0.075175 2.1:-1348723 None:intergenic
CTTCGTTGAATATGAATAAA+AGG 0.247349 2.1:+1348585 MS.gene85933:CDS
AGAAAATACTGAAAATTCTT+TGG 0.251631 2.1:+1349819 MS.gene85933:CDS
AGATCCAGCAAAACTTGTTT+TGG 0.272394 2.1:+1349624 MS.gene85933:CDS
ACAGCTTTGTCTCCCTTCTT+AGG 0.285668 2.1:-1349676 None:intergenic
CTGCAAAGAGCTAGAAAATA+AGG 0.286190 2.1:+1348748 MS.gene85933:CDS
GAGTCAAAGCTGAATAATTT+TGG 0.290126 2.1:+1349118 MS.gene85933:CDS
CTTACACAAGATTTAATATC+TGG 0.303638 2.1:-1349751 None:intergenic
GATGGAACAAGTGAGGAATT+TGG 0.315869 2.1:+1349574 MS.gene85933:CDS
TAGAGGGATTGCATCATCTT+TGG 0.317901 2.1:+1352473 MS.gene85933:CDS
ACAATACTTGTGCGTGATTC+GGG 0.321400 2.1:-1351389 None:intergenic
CAGACTCAAGTTCCTTAACT+TGG 0.324284 2.1:-1348775 None:intergenic
AGATAGCTGTAAAGCTGTTT+AGG 0.328283 2.1:+1349950 MS.gene85933:CDS
TTCAATTTCGTACTAGTGAT+TGG 0.332218 2.1:+1351345 MS.gene85933:CDS
TCCTTTCAGAGTCCTGTGAT+TGG 0.368551 2.1:+1351318 MS.gene85933:intron
TCAAAAGAGGAGGAATTTAA+AGG 0.372635 2.1:+1348962 MS.gene85933:CDS
CTTGGAAAGCAACAATTCCT+TGG 0.374250 2.1:+1348496 MS.gene85933:CDS
CAATTCAGTATCACCATTGA+TGG 0.374340 2.1:+1349556 MS.gene85933:CDS
CTGTCAAATAACCTCTTAAC+TGG 0.388338 2.1:-1348464 None:intergenic
GACAATACTTGTGCGTGATT+CGG 0.388703 2.1:-1351390 None:intergenic
TTATCGATGGCGACGATGTC+AGG 0.391493 2.1:+1348012 None:intergenic
GAAAATTCTTTGGAGGTTCT+CGG 0.397484 2.1:+1349829 MS.gene85933:CDS
TTCCTCACTTGTTCCATCAA+TGG 0.405596 2.1:-1349569 None:intergenic
AATTTCAGTATGATTACAAC+TGG 0.408474 2.1:+1352642 MS.gene85933:intron
TTGTCTCCCTTCTTAGGCAG+TGG 0.411661 2.1:-1349670 None:intergenic
TTCTTAGGCAGTGGAATCGT+GGG 0.412112 2.1:-1349661 None:intergenic
TCTGAAAAGAATATACTTGA+TGG 0.418087 2.1:+1348794 MS.gene85933:CDS
TTGATTCGTTGGTAAATTGT+TGG 0.419166 2.1:-1352723 None:intergenic
CCTCTTGGTTGTGGTGTAGA+GGG 0.421601 2.1:+1352457 MS.gene85933:CDS
TTCGTACTAGTGATTGGAGT+TGG 0.431108 2.1:+1351351 MS.gene85933:CDS
GAAGGAAGAAGTAGATCAAA+AGG 0.433938 2.1:+1348324 MS.gene85933:CDS
AATTGTTGGCCACAGGGTCC+TGG 0.438116 2.1:-1352709 None:intergenic
TTGGATTTAACAGCACGAAC+TGG 0.439874 2.1:+1348280 MS.gene85933:intron
CTTTGAGATTGTCCTCAGCT+TGG 0.447230 2.1:-1349048 None:intergenic
AAATAAGGAGACCCAAGTTA+AGG 0.448359 2.1:+1348763 MS.gene85933:CDS
ATTTGACAGCAACAATTCCT+TGG 0.450325 2.1:+1348478 MS.gene85933:CDS
CAAGGAATTGTTGCTTTCCA+AGG 0.451728 2.1:-1348495 None:intergenic
ACATTTCGCGAGCCGACAAA+AGG 0.460184 2.1:+1348889 MS.gene85933:CDS
GGAATTTGAATCAAAAGAAA+AGG 0.461737 2.1:+1348910 MS.gene85933:CDS
CTACTGCACTGCCAGTTAAG+AGG 0.461854 2.1:+1348453 MS.gene85933:CDS
TGGCCACAGGGTCCTGGACC+CGG 0.463853 2.1:-1352703 None:intergenic
CACGATTCCACTGCCTAAGA+AGG 0.465950 2.1:+1349663 MS.gene85933:CDS
TCCTCTTGGTTGTGGTGTAG+AGG 0.467927 2.1:+1352456 MS.gene85933:CDS
TCAGATTGAAGTACAAATCA+AGG 0.469530 2.1:+1349090 MS.gene85933:CDS
GTTGGTAAATTGTTGGCCAC+AGG 0.469858 2.1:-1352716 None:intergenic
CTGATTCAAAGTTCTTCACT+AGG 0.474701 2.1:-1348859 None:intergenic
AAATTATTCAGCTTTGACTC+AGG 0.479176 2.1:-1349115 None:intergenic
GTAAAGCTGTTTAGGACCCT+GGG 0.479502 2.1:+1349958 MS.gene85933:CDS
AAATACTGAAAATTCTTTGG+AGG 0.482682 2.1:+1349822 MS.gene85933:CDS
TGTAAAGCTGTTTAGGACCC+TGG 0.484740 2.1:+1349957 MS.gene85933:CDS
AATCATTTGAGGAATGCAAC+GGG 0.489095 2.1:+1348669 MS.gene85933:CDS
AGTGTTTGAAGCAAAGGAAG+AGG 0.504885 2.1:+1348994 MS.gene85933:CDS
GAATTATCAGCTGATTTGCC+TGG 0.507013 2.1:-1348608 None:intergenic
GGAATTTGAAAGAAGAGTGA+AGG 0.507055 2.1:+1348931 MS.gene85933:CDS
AGATGTCTTCCTCTTGGTTG+TGG 0.512724 2.1:+1352448 MS.gene85933:CDS
GTTTACAGCTGCAACAAGTC+AGG 0.515862 2.1:+1352525 MS.gene85933:CDS
TTGAATATGAATAAAAGGCC+AGG 0.518437 2.1:+1348590 MS.gene85933:CDS
AATTTACCAACGAATCAAGT+TGG 0.519521 2.1:+1352728 MS.gene85933:CDS
CTAGGGCCAACTTGATTCGT+TGG 0.520186 2.1:-1352734 None:intergenic
AGACTCAAGTTCCTTAACTT+GGG 0.522775 2.1:-1348774 None:intergenic
CACGAACTGGACAGCCAAGT+GGG 0.524603 2.1:+1348293 MS.gene85933:CDS
CTTCTTAGGCAGTGGAATCG+TGG 0.525924 2.1:-1349662 None:intergenic
ACATCTTCTCTTCAAACCGC+AGG 0.530472 2.1:-1352504 None:intergenic
GATGGCTAGACACCGGGTCC+AGG 0.535555 2.1:+1352691 MS.gene85933:CDS
GCTAACCTGGATCAGAGGCT+AGG 0.558312 2.1:-1352752 None:intergenic
AAATAGAAGAGTGGCGATTG+TGG 0.559419 2.1:+1348699 MS.gene85933:CDS
TTGGTAAATTGTTGGCCACA+GGG 0.565907 2.1:-1352715 None:intergenic
CTAACCTGGATCAGAGGCTA+GGG 0.567094 2.1:-1352751 None:intergenic
GAAGGAGTTCCAGTCAAAAG+AGG 0.567276 2.1:+1348949 MS.gene85933:CDS
AGTGAAAGTGTTTGAAGCAA+AGG 0.574401 2.1:+1348988 MS.gene85933:CDS
TTTCTTAACAGAAAATTCCA+AGG 0.575654 2.1:-1348513 None:intergenic
AAATCATTTGAGGAATGCAA+CGG 0.576495 2.1:+1348668 MS.gene85933:CDS
TCAAACGATGGCTAGACACC+GGG 0.587136 2.1:+1352685 MS.gene85933:CDS
CATCTTCTCTTCAAACCGCA+GGG 0.594174 2.1:-1352503 None:intergenic
ACGATTCCACTGCCTAAGAA+GGG 0.594525 2.1:+1349664 MS.gene85933:CDS
ATATGCAGATACATCTAACA+TGG 0.600498 2.1:+1349528 MS.gene85933:intron
TAGTAAGTTGCTTCAAACGA+TGG 0.610073 2.1:+1352673 MS.gene85933:CDS
AAATTGAACTCTCCAATCAC+AGG 0.611839 2.1:-1351330 None:intergenic
TTCAAACGATGGCTAGACAC+CGG 0.617499 2.1:+1352684 MS.gene85933:CDS
GGAGTTCCAGTCAAAAGAGG+AGG 0.625446 2.1:+1348952 MS.gene85933:CDS
ACGAACTGGACAGCCAAGTG+GGG 0.626248 2.1:+1348294 MS.gene85933:CDS
GACTTTATGTACAAAACCCA+GGG 0.629177 2.1:-1349974 None:intergenic
GCAAAGGAAGAGGAATTCGA+AGG 0.634363 2.1:+1349004 MS.gene85933:CDS
CACCATTGATGGAACAAGTG+AGG 0.634662 2.1:+1349567 MS.gene85933:CDS
TGCAAGTTCTTTCCTCAGCG+AGG 0.638740 2.1:-1348636 None:intergenic
AGACTTTATGTACAAAACCC+AGG 0.639555 2.1:-1349975 None:intergenic
GGAATCAACAAATAGAAGAG+TGG 0.642926 2.1:+1348690 MS.gene85933:CDS
GCACGAACTGGACAGCCAAG+TGG 0.645283 2.1:+1348292 MS.gene85933:intron
ACTAGTGATTGGAGTTGGAG+AGG 0.652499 2.1:+1351356 MS.gene85933:CDS
CCCTCTACACCACAACCAAG+AGG 0.653097 2.1:-1352457 None:intergenic
GCAATTTGAAAACCAAGCTG+AGG 0.662165 2.1:+1349036 MS.gene85933:CDS
CGAACTGGACAGCCAAGTGG+GGG 0.663711 2.1:+1348295 MS.gene85933:CDS
TCTTAGGCAGTGGAATCGTG+GGG 0.670339 2.1:-1349660 None:intergenic
ACACCGGGTCCAGGACCCTG+TGG 0.680021 2.1:+1352700 MS.gene85933:CDS
ATCTTTGGAAGATTGCCCTG+CGG 0.716047 2.1:+1352488 MS.gene85933:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATATATATTATGATAAA+CGG - chr2.1:1348251-1348270 None:intergenic 10.0%
!! TATATATGTTATAGATTATT+TGG + chr2.1:1348261-1348280 MS.gene85933:intron 10.0%
!! AGAAAAATATATGTATGATA+AGG + chr2.1:1351577-1351596 MS.gene85933:intron 15.0%
!! ATACGAATATAACATTTAAA+AGG - chr2.1:1351279-1351298 None:intergenic 15.0%
!! ATTAATAAGATATTAGATCT+AGG + chr2.1:1350401-1350420 MS.gene85933:intron 15.0%
!! TTAACAAAAAACTAACAAAT+TGG - chr2.1:1350618-1350637 None:intergenic 15.0%
!!! CTTTTGTATTTTAATTTTCA+AGG + chr2.1:1350844-1350863 MS.gene85933:intron 15.0%
!!! TTCTTTTCTTATAAAAAAAG+AGG - chr2.1:1351561-1351580 None:intergenic 15.0%
!! AAAGAAATAAGAGCTAATAT+GGG + chr2.1:1351009-1351028 MS.gene85933:intron 20.0%
!! AACATTTAAAAGGTAATTAG+TGG - chr2.1:1351269-1351288 None:intergenic 20.0%
!! AATGAAATAAGATGATTATC+AGG - chr2.1:1348120-1348139 None:intergenic 20.0%
!! AGAAAATACTGAAAATTCTT+TGG + chr2.1:1349819-1349838 MS.gene85933:CDS 20.0%
!! AGAAATTTGATTCAGTTAAT+TGG + chr2.1:1350725-1350744 MS.gene85933:intron 20.0%
!! ATTGTTGAAAAAATCATTTG+AGG + chr2.1:1348658-1348677 MS.gene85933:CDS 20.0%
!! ATTTCTTAACAAAATTTGCA+AGG - chr2.1:1350697-1350716 None:intergenic 20.0%
!! TAAATTCTGTAGTTAAATGA+TGG + chr2.1:1348400-1348419 MS.gene85933:intron 20.0%
!! TATGATGAAGAAAAAGAATT+TGG + chr2.1:1349178-1349197 MS.gene85933:CDS 20.0%
!! TATTAATCTTAGTATCTTTG+TGG - chr2.1:1350388-1350407 None:intergenic 20.0%
!! TTAGATACAAAATTGCAATA+TGG - chr2.1:1350090-1350109 None:intergenic 20.0%
!! TTGTAATCATACTGAAATTA+GGG - chr2.1:1352641-1352660 None:intergenic 20.0%
!!! ACGAATCAATTTTCAAATTT+CGG + chr2.1:1351146-1351165 MS.gene85933:intron 20.0%
!!! ATAATAAATTGATAATCACG+AGG - chr2.1:1352569-1352588 None:intergenic 20.0%
!!! ATTTTCTGTTAAGAAATCAA+AGG + chr2.1:1348520-1348539 MS.gene85933:CDS 20.0%
!!! TAAAACTAAAGCTTACATTT+TGG - chr2.1:1351417-1351436 None:intergenic 20.0%
!!! TATCTCTTTCATTTTCTAAA+TGG + chr2.1:1350567-1350586 MS.gene85933:intron 20.0%
!!! TCTCAATTTCTTATGTTTTA+TGG + chr2.1:1352100-1352119 MS.gene85933:intron 20.0%
!!! TCTTTTATGTTGTTTTAGAT+TGG + chr2.1:1350115-1350134 MS.gene85933:intron 20.0%
!!! TGTAAGCTTTAGTTTTAATA+CGG + chr2.1:1351420-1351439 MS.gene85933:intron 20.0%
!!! TTCTTATGTTTTATGGAAAA+TGG + chr2.1:1352107-1352126 MS.gene85933:intron 20.0%
! AAATACTGAAAATTCTTTGG+AGG + chr2.1:1349822-1349841 MS.gene85933:CDS 25.0%
! AAATGAGCTTGAATCAAAAA+AGG + chr2.1:1348823-1348842 MS.gene85933:CDS 25.0%
! AACAATTGACAAAACTGTTT+GGG - chr2.1:1350474-1350493 None:intergenic 25.0%
! AAGGATAAACATTCGATAAA+TGG - chr2.1:1348177-1348196 None:intergenic 25.0%
! AATCAGGATAGCATATAATA+GGG + chr2.1:1348043-1348062 MS.gene85933:intron 25.0%
! AATGAGCTTGAATCAAAAAA+GGG + chr2.1:1348824-1348843 MS.gene85933:CDS 25.0%
! AATTTCAGTATGATTACAAC+TGG + chr2.1:1352642-1352661 MS.gene85933:intron 25.0%
! ATAGAAACTTAGTGAATCAT+TGG - chr2.1:1349371-1349390 None:intergenic 25.0%
! ATGATAAGTAAAAACTATGC+AGG + chr2.1:1350358-1350377 MS.gene85933:intron 25.0%
! CAAAGAAATAAGAGCTAATA+TGG + chr2.1:1351008-1351027 MS.gene85933:intron 25.0%
! CTTACACAAGATTTAATATC+TGG - chr2.1:1349754-1349773 None:intergenic 25.0%
! CTTATTCAATTTAGACCTTT+AGG + chr2.1:1351915-1351934 MS.gene85933:intron 25.0%
! CTTCGTTGAATATGAATAAA+AGG + chr2.1:1348585-1348604 MS.gene85933:CDS 25.0%
! CTTCTCAATATTTCTCTTTA+TGG - chr2.1:1348726-1348745 None:intergenic 25.0%
! GGAATTTGAATCAAAAGAAA+AGG + chr2.1:1348910-1348929 MS.gene85933:CDS 25.0%
! GGTCATATTCTATTATATGA+GGG + chr2.1:1352337-1352356 MS.gene85933:intron 25.0%
! GTTGTAATCATACTGAAATT+AGG - chr2.1:1352642-1352661 None:intergenic 25.0%
! TAACAATTGACAAAACTGTT+TGG - chr2.1:1350475-1350494 None:intergenic 25.0%
! TATGGAAAATGGATGTTTAT+TGG + chr2.1:1352118-1352137 MS.gene85933:intron 25.0%
! TCTGAAAAGAATATACTTGA+TGG + chr2.1:1348794-1348813 MS.gene85933:CDS 25.0%
! TCTTGATATGTTATTGTCAT+TGG - chr2.1:1352367-1352386 None:intergenic 25.0%
! TGTAGAACATATTATTTGTG+TGG + chr2.1:1350271-1350290 MS.gene85933:intron 25.0%
! TTAAGATGGCAAAAAATTGA+AGG - chr2.1:1348196-1348215 None:intergenic 25.0%
! TTATAGCCAAAACTACAAAT+AGG - chr2.1:1349326-1349345 None:intergenic 25.0%
! TTATCAGGATTTAAAGAAAC+AGG - chr2.1:1348105-1348124 None:intergenic 25.0%
! TTTCTTAACAGAAAATTCCA+AGG - chr2.1:1348516-1348535 None:intergenic 25.0%
!! AAAAACTTGTTCATTCTTGT+CGG - chr2.1:1350751-1350770 None:intergenic 25.0%
!! AATAGTCCTATTTGTAGTTT+TGG + chr2.1:1349317-1349336 MS.gene85933:intron 25.0%
!! ATATATACTATGAAAGCATG+TGG - chr2.1:1349428-1349447 None:intergenic 25.0%
!! CATTTCAACAACAACTTTTA+TGG + chr2.1:1349238-1349257 MS.gene85933:intron 25.0%
!! CATTTTCACATTCAACTTAA+AGG - chr2.1:1350937-1350956 None:intergenic 25.0%
!! CTTTATTTCTTCAGTGAATA+AGG + chr2.1:1348366-1348385 MS.gene85933:intron 25.0%
!! CTTTTATTCATATTCAACGA+AGG - chr2.1:1348587-1348606 None:intergenic 25.0%
!! TAAGAGAAAATTGACTTCTT+GGG + chr2.1:1350493-1350512 MS.gene85933:intron 25.0%
!! TTAAGAGAAAATTGACTTCT+TGG + chr2.1:1350492-1350511 MS.gene85933:intron 25.0%
!! TTGATTCAAATTCCTTTTGT+CGG - chr2.1:1348904-1348923 None:intergenic 25.0%
!! TTTTCAAATTTCGGTTAAGT+TGG + chr2.1:1351155-1351174 MS.gene85933:intron 25.0%
!!! CATCTAAAAGTATTTGACTT+TGG - chr2.1:1350341-1350360 None:intergenic 25.0%
!!! CATTTAAACTTCTAGAATGT+AGG - chr2.1:1350656-1350675 None:intergenic 25.0%
!!! GATTGGCTTTAAATTTTAGT+TGG + chr2.1:1350132-1350151 MS.gene85933:intron 25.0%
AAATCATTTGAGGAATGCAA+CGG + chr2.1:1348668-1348687 MS.gene85933:CDS 30.0%
AATTTACCAACGAATCAAGT+TGG + chr2.1:1352728-1352747 MS.gene85933:CDS 30.0%
ACAATTGACAAAACTGTTTG+GGG - chr2.1:1350473-1350492 None:intergenic 30.0%
ACATGAGCAACAAAAATTGA+AGG - chr2.1:1349472-1349491 None:intergenic 30.0%
ATATGCAGATACATCTAACA+TGG + chr2.1:1349528-1349547 MS.gene85933:intron 30.0%
ATTATGATAAACGGTACAAC+AGG - chr2.1:1348242-1348261 None:intergenic 30.0%
ATTGATATGATTCCATAGAC+TGG + chr2.1:1351513-1351532 MS.gene85933:intron 30.0%
CAAAAATTGAAGGTGACAAT+AGG - chr2.1:1349462-1349481 None:intergenic 30.0%
CAAAATTGAACTCATGCAAT+TGG + chr2.1:1350434-1350453 MS.gene85933:intron 30.0%
CAATCAGGATAGCATATAAT+AGG + chr2.1:1348042-1348061 MS.gene85933:intron 30.0%
CGGTCATATTCTATTATATG+AGG + chr2.1:1352336-1352355 MS.gene85933:intron 30.0%
GATATTAGATCTAGGTAACT+TGG + chr2.1:1350409-1350428 MS.gene85933:intron 30.0%
GCTACAAAAATGAAAAACAG+TGG - chr2.1:1352413-1352432 None:intergenic 30.0%
GTTGCCAAGAAAATTTCTTA+AGG + chr2.1:1352179-1352198 MS.gene85933:intron 30.0%
TAATGCTTCTGTTTAGAAGA+TGG + chr2.1:1348427-1348446 MS.gene85933:intron 30.0%
TATGTGTGTGATATAGAAAC+TGG - chr2.1:1349298-1349317 None:intergenic 30.0%
TATTGTTGAAACCTGCTAAT+AGG + chr2.1:1350785-1350804 MS.gene85933:intron 30.0%
TCAAAAAAGGGTGAATTTGA+TGG + chr2.1:1348836-1348855 MS.gene85933:CDS 30.0%
TCAAAAGAGGAGGAATTTAA+AGG + chr2.1:1348962-1348981 MS.gene85933:CDS 30.0%
TCAGATTGAAGTACAAATCA+AGG + chr2.1:1349090-1349109 MS.gene85933:CDS 30.0%
TCTTAACAAAATTTGCAAGG+TGG - chr2.1:1350694-1350713 None:intergenic 30.0%
TGTACAAATGTTCCTGAAAT+AGG + chr2.1:1350295-1350314 MS.gene85933:intron 30.0%
TTAAAAAGATGTCTTCCTCT+TGG + chr2.1:1352442-1352461 MS.gene85933:CDS 30.0%
TTATGATAAACGGTACAACA+GGG - chr2.1:1348241-1348260 None:intergenic 30.0%
TTCAATTTCGTACTAGTGAT+TGG + chr2.1:1351345-1351364 MS.gene85933:CDS 30.0%
TTGAATATGAATAAAAGGCC+AGG + chr2.1:1348590-1348609 MS.gene85933:CDS 30.0%
! AAATTATTCAGCTTTGACTC+AGG - chr2.1:1349118-1349137 None:intergenic 30.0%
! ATATCCAAAACAAGTTTTGC+TGG - chr2.1:1349631-1349650 None:intergenic 30.0%
! CAATCTGATTCTCTTTTAAC+TGG - chr2.1:1349078-1349097 None:intergenic 30.0%
! GAAATTTTCTTGGCAACATT+AGG - chr2.1:1352176-1352195 None:intergenic 30.0%
! GAGTCAAAGCTGAATAATTT+TGG + chr2.1:1349118-1349137 MS.gene85933:CDS 30.0%
! GCAACATTTCATTAGGTTTT+CGG + chr2.1:1351535-1351554 MS.gene85933:intron 30.0%
! TAGCTCTTATTTCTTTGAGA+TGG - chr2.1:1351005-1351024 None:intergenic 30.0%
! TCAAAGCTGAATAATTTTGG+TGG + chr2.1:1349121-1349140 MS.gene85933:CDS 30.0%
! TCCATTCTACTTCCTATTTT+TGG + chr2.1:1352261-1352280 MS.gene85933:intron 30.0%
! TCCTCCTTAAGAAATTTTCT+TGG - chr2.1:1352186-1352205 None:intergenic 30.0%
! TTGATTCGTTGGTAAATTGT+TGG - chr2.1:1352726-1352745 None:intergenic 30.0%
! TTTGCCATCTTAATGCTTTT+GGG + chr2.1:1348203-1348222 MS.gene85933:intron 30.0%
! TTTTGCCATCTTAATGCTTT+TGG + chr2.1:1348202-1348221 MS.gene85933:intron 30.0%
!! TAATTTTGGTGGACAACTAA+AGG + chr2.1:1349132-1349151 MS.gene85933:CDS 30.0%
!! TATTCCCAAAAGCATTAAGA+TGG - chr2.1:1348210-1348229 None:intergenic 30.0%
!! TGTTTTTCATTTTTGTAGCG+TGG + chr2.1:1352414-1352433 MS.gene85933:intron 30.0%
!! TTAATGCTTTTGGGAATATG+CGG + chr2.1:1348212-1348231 MS.gene85933:intron 30.0%
!!! ATTCTACTTCCTATTTTTGG+CGG + chr2.1:1352264-1352283 MS.gene85933:intron 30.0%
!!! CTCTTATTTTATCCTCACTT+TGG - chr2.1:1351206-1351225 None:intergenic 30.0%
!!! CTGATGATTTTGTCGATTTA+TGG + chr2.1:1349856-1349875 MS.gene85933:CDS 30.0%
AAATAAGGAGACCCAAGTTA+AGG + chr2.1:1348763-1348782 MS.gene85933:CDS 35.0%
AAATTGAACTCTCCAATCAC+AGG - chr2.1:1351333-1351352 None:intergenic 35.0%
AACAAGAGTTGTCCTATTTC+AGG - chr2.1:1350310-1350329 None:intergenic 35.0%
AATCATTTGAGGAATGCAAC+GGG + chr2.1:1348669-1348688 MS.gene85933:CDS 35.0%
AATGAAATGTTGCCAGTCTA+TGG - chr2.1:1351528-1351547 None:intergenic 35.0%
ACTTCTTGGGTGTTAATGTT+TGG + chr2.1:1350506-1350525 MS.gene85933:intron 35.0%
AGACTCAAGTTCCTTAACTT+GGG - chr2.1:1348777-1348796 None:intergenic 35.0%
AGACTTTATGTACAAAACCC+AGG - chr2.1:1349978-1349997 None:intergenic 35.0%
AGATAGCTGTAAAGCTGTTT+AGG + chr2.1:1349950-1349969 MS.gene85933:CDS 35.0%
AGTAGTCAAATCCTATTAGC+AGG - chr2.1:1350799-1350818 None:intergenic 35.0%
AGTCCATAATCCACATTCTA+CGG - chr2.1:1351888-1351907 None:intergenic 35.0%
AGTGCAAAAATCAAGGTTCA+CGG - chr2.1:1348079-1348098 None:intergenic 35.0%
ATAAACACCAGTTTCATGTG+TGG + chr2.1:1351849-1351868 MS.gene85933:intron 35.0%
ATGCATACAAAGATAGCACA+TGG - chr2.1:1351976-1351995 None:intergenic 35.0%
ATTTGACAGCAACAATTCCT+TGG + chr2.1:1348478-1348497 MS.gene85933:CDS 35.0%
CAATTCAGTATCACCATTGA+TGG + chr2.1:1349556-1349575 MS.gene85933:CDS 35.0%
CACTTCGTAGTCATTTCATA+TGG + chr2.1:1351743-1351762 MS.gene85933:intron 35.0%
CAGGATAAGTGCAAAAATCA+AGG - chr2.1:1348086-1348105 None:intergenic 35.0%
CATATGAAATGACTACGAAG+TGG - chr2.1:1351745-1351764 None:intergenic 35.0%
CTGATTCAAAGTTCTTCACT+AGG - chr2.1:1348862-1348881 None:intergenic 35.0%
CTGCAAAGAGCTAGAAAATA+AGG + chr2.1:1348748-1348767 MS.gene85933:CDS 35.0%
CTGTCAAATAACCTCTTAAC+TGG - chr2.1:1348467-1348486 None:intergenic 35.0%
GAAGGAAGAAGTAGATCAAA+AGG + chr2.1:1348324-1348343 MS.gene85933:CDS 35.0%
GACTTTATGTACAAAACCCA+GGG - chr2.1:1349977-1349996 None:intergenic 35.0%
GCACATGGAAAACATAAACT+TGG - chr2.1:1351961-1351980 None:intergenic 35.0%
GCCAAAAATAGGAAGTAGAA+TGG - chr2.1:1352265-1352284 None:intergenic 35.0%
GCCAAGAAAATTTCTTAAGG+AGG + chr2.1:1352182-1352201 MS.gene85933:intron 35.0%
GGAATCAACAAATAGAAGAG+TGG + chr2.1:1348690-1348709 MS.gene85933:CDS 35.0%
GGAATTTGAAAGAAGAGTGA+AGG + chr2.1:1348931-1348950 MS.gene85933:CDS 35.0%
GTATCATATTCGAAGTCTGA+AGG - chr2.1:1351799-1351818 None:intergenic 35.0%
TAAACACCAGTTTCATGTGT+GGG + chr2.1:1351850-1351869 MS.gene85933:intron 35.0%
TAGACTGGCAACATTTCATT+AGG + chr2.1:1351528-1351547 MS.gene85933:intron 35.0%
TAGGATTTGACTACTAATGC+TGG + chr2.1:1350804-1350823 MS.gene85933:intron 35.0%
TAGTAAGTTGCTTCAAACGA+TGG + chr2.1:1352673-1352692 MS.gene85933:CDS 35.0%
TCAACTGATGTTGTAATCGA+TGG - chr2.1:1351088-1351107 None:intergenic 35.0%
TCTTATCTTGCATTTGCAAG+AGG + chr2.1:1351698-1351717 MS.gene85933:intron 35.0%
TTTAGACCTTTAGGTCTTGA+AGG + chr2.1:1351924-1351943 MS.gene85933:intron 35.0%
! ACTCTGAAAGGAAGGTATAT+AGG - chr2.1:1351310-1351329 None:intergenic 35.0%
! ATAATTCATTTTCCTCGCTG+AGG + chr2.1:1348624-1348643 MS.gene85933:CDS 35.0%
! GAAAATTCTTTGGAGGTTCT+CGG + chr2.1:1349829-1349848 MS.gene85933:CDS 35.0%
! TAAATTCCTCCTCTTTTGAC+TGG - chr2.1:1348961-1348980 None:intergenic 35.0%
!! AGATCCAGCAAAACTTGTTT+TGG + chr2.1:1349624-1349643 MS.gene85933:CDS 35.0%
!! AGTGAAAGTGTTTGAAGCAA+AGG + chr2.1:1348988-1349007 MS.gene85933:CDS 35.0%
!! ATGTCAGGTTAGTTTCAATC+AGG + chr2.1:1348027-1348046 MS.gene85933:intron 35.0%
!! GCTAATATGGGTATTGAGAT+TGG + chr2.1:1351021-1351040 MS.gene85933:intron 35.0%
!! GGTTTTGTACATAAAGTCTC+TGG + chr2.1:1349979-1349998 MS.gene85933:CDS 35.0%
!! GTTTTTCTAGCAGATAGATG+CGG - chr2.1:1349717-1349736 None:intergenic 35.0%
!! TTTGAGATGGATTGTAAGAG+TGG - chr2.1:1350992-1351011 None:intergenic 35.0%
!!! ATGAGTTTTGAACAAGAACC+TGG + chr2.1:1352025-1352044 MS.gene85933:intron 35.0%
!!! GCAGTTTTAGCTTTTCAGTT+TGG + chr2.1:1351225-1351244 MS.gene85933:intron 35.0%
!!! GTTTTTTGCATGCTGTTTCA+GGG + chr2.1:1352048-1352067 MS.gene85933:intron 35.0%
!!! TGAGTTTTGAACAAGAACCT+GGG + chr2.1:1352026-1352045 MS.gene85933:intron 35.0%
!!! TTCTACTTCCTATTTTTGGC+GGG + chr2.1:1352265-1352284 MS.gene85933:intron 35.0%
AAACAGCATGCAAAAAACCC+AGG - chr2.1:1352046-1352065 None:intergenic 40.0%
AAATAGAAGAGTGGCGATTG+TGG + chr2.1:1348699-1348718 MS.gene85933:CDS 40.0%
AAGAACTCGCTAAAGTCATC+TGG - chr2.1:1352307-1352326 None:intergenic 40.0%
ACAATACTTGTGCGTGATTC+GGG - chr2.1:1351392-1351411 None:intergenic 40.0%
ATGAAATGACTACGAAGTGG+AGG - chr2.1:1351742-1351761 None:intergenic 40.0%
CAGACTCAAGTTCCTTAACT+TGG - chr2.1:1348778-1348797 None:intergenic 40.0%
CTTGGAAAGCAACAATTCCT+TGG + chr2.1:1348496-1348515 MS.gene85933:CDS 40.0%
GACAATACTTGTGCGTGATT+CGG - chr2.1:1351393-1351412 None:intergenic 40.0%
GATGGAACAAGTGAGGAATT+TGG + chr2.1:1349574-1349593 MS.gene85933:CDS 40.0%
GCAATTTGAAAACCAAGCTG+AGG + chr2.1:1349036-1349055 MS.gene85933:CDS 40.0%
GTCCATAATCCACATTCTAC+GGG - chr2.1:1351887-1351906 None:intergenic 40.0%
GTGAGTCTTGACACAACAAA+TGG - chr2.1:1351777-1351796 None:intergenic 40.0%
GTGCTATCTTTGTATGCATG+AGG + chr2.1:1351977-1351996 MS.gene85933:intron 40.0%
GTTATCTGTACTAACGCACA+TGG + chr2.1:1352590-1352609 MS.gene85933:intron 40.0%
TAGAGGGATTGCATCATCTT+TGG + chr2.1:1352473-1352492 MS.gene85933:CDS 40.0%
TCATTATCCCCGCCAAAAAT+AGG - chr2.1:1352276-1352295 None:intergenic 40.0%
TCCATAATCCACATTCTACG+GGG - chr2.1:1351886-1351905 None:intergenic 40.0%
TGTATGATAAGGCTCATGCA+CGG + chr2.1:1351588-1351607 MS.gene85933:intron 40.0%
TTCCTCACTTGTTCCATCAA+TGG - chr2.1:1349572-1349591 None:intergenic 40.0%
TTCGTACTAGTGATTGGAGT+TGG + chr2.1:1351351-1351370 MS.gene85933:CDS 40.0%
TTGGATTTAACAGCACGAAC+TGG + chr2.1:1348280-1348299 MS.gene85933:intron 40.0%
TTGGTAAATTGTTGGCCACA+GGG - chr2.1:1352718-1352737 None:intergenic 40.0%
! AGTGTTTGAAGCAAAGGAAG+AGG + chr2.1:1348994-1349013 MS.gene85933:CDS 40.0%
! TAGCTTTTCTCTCCAAAGTG+AGG + chr2.1:1351191-1351210 MS.gene85933:intron 40.0%
!! CAAGGAATTGTTGCTTTCCA+AGG - chr2.1:1348498-1348517 None:intergenic 40.0%
!! GAATTATCAGCTGATTTGCC+TGG - chr2.1:1348611-1348630 None:intergenic 40.0%
!!! ACTGGACTAGCTTTTCAACT+TGG - chr2.1:1349280-1349299 None:intergenic 40.0%
!!! GGTTTTTTGCATGCTGTTTC+AGG + chr2.1:1352047-1352066 MS.gene85933:intron 40.0%
!!! TCTACTTCCTATTTTTGGCG+GGG + chr2.1:1352266-1352285 MS.gene85933:intron 40.0%
ACAATGCCCACACATGAAAC+TGG - chr2.1:1351859-1351878 None:intergenic 45.0%
ACATCTTCTCTTCAAACCGC+AGG - chr2.1:1352507-1352526 None:intergenic 45.0%
ACGATTCCACTGCCTAAGAA+GGG + chr2.1:1349664-1349683 MS.gene85933:CDS 45.0%
ACTAGTGATTGGAGTTGGAG+AGG + chr2.1:1351356-1351375 MS.gene85933:CDS 45.0%
AGACCTTTAGGTCTTGAAGG+AGG + chr2.1:1351927-1351946 MS.gene85933:intron 45.0%
AGATGTCTTCCTCTTGGTTG+TGG + chr2.1:1352448-1352467 MS.gene85933:CDS 45.0%
AGCGAGTTCTTCTTGCATGT+CGG + chr2.1:1352316-1352335 MS.gene85933:intron 45.0%
AGTAGAATGGACGTCAGACA+TGG - chr2.1:1352252-1352271 None:intergenic 45.0%
CACCATTGATGGAACAAGTG+AGG + chr2.1:1349567-1349586 MS.gene85933:CDS 45.0%
CATCTTCTCTTCAAACCGCA+GGG - chr2.1:1352506-1352525 None:intergenic 45.0%
CGAAGTGGAGGTTTGATTAG+TGG - chr2.1:1351730-1351749 None:intergenic 45.0%
GAAGGAGTTCCAGTCAAAAG+AGG + chr2.1:1348949-1348968 MS.gene85933:CDS 45.0%
GCAAAGGAAGAGGAATTCGA+AGG + chr2.1:1349004-1349023 MS.gene85933:CDS 45.0%
GTACTATAAAGCTCCCCTCA+TGG + chr2.1:1351637-1351656 MS.gene85933:intron 45.0%
GTTTACAGCTGCAACAAGTC+AGG + chr2.1:1352525-1352544 MS.gene85933:CDS 45.0%
TAGATTCACAACAGAGCTCC+CGG - chr2.1:1351669-1351688 None:intergenic 45.0%
TCCAATCACAGGACTCTGAA+AGG - chr2.1:1351322-1351341 None:intergenic 45.0%
TCCTTTCAGAGTCCTGTGAT+TGG + chr2.1:1351318-1351337 MS.gene85933:intron 45.0%
TCTCCTCCTTCAAGACCTAA+AGG - chr2.1:1351933-1351952 None:intergenic 45.0%
TTCAAACGATGGCTAGACAC+CGG + chr2.1:1352684-1352703 MS.gene85933:CDS 45.0%
TTCTTAGGCAGTGGAATCGT+GGG - chr2.1:1349664-1349683 None:intergenic 45.0%
TTGTATGCATGAGGCGTGTT+TGG + chr2.1:1351986-1352005 MS.gene85933:intron 45.0%
! ACAGCTTTGTCTCCCTTCTT+AGG - chr2.1:1349679-1349698 None:intergenic 45.0%
! ATCACAGGACTCTGAAAGGA+AGG - chr2.1:1351318-1351337 None:intergenic 45.0%
! ATCTTTGGAAGATTGCCCTG+CGG + chr2.1:1352488-1352507 MS.gene85933:CDS 45.0%
! CTGTTTCAGGGTTGTGGTTT+TGG + chr2.1:1352060-1352079 MS.gene85933:intron 45.0%
! GTAAAGCTGTTTAGGACCCT+GGG + chr2.1:1349958-1349977 MS.gene85933:CDS 45.0%
! GTTGGTAAATTGTTGGCCAC+AGG - chr2.1:1352719-1352738 None:intergenic 45.0%
! TCCCCGTAGAATGTGGATTA+TGG + chr2.1:1351882-1351901 MS.gene85933:intron 45.0%
! TGTAAAGCTGTTTAGGACCC+TGG + chr2.1:1349957-1349976 MS.gene85933:CDS 45.0%
!! CTTTGAGATTGTCCTCAGCT+TGG - chr2.1:1349051-1349070 None:intergenic 45.0%
ACATTTCGCGAGCCGACAAA+AGG + chr2.1:1348889-1348908 MS.gene85933:CDS 50.0%
ATGCATGAGGCGTGTTTGGT+TGG + chr2.1:1351990-1352009 MS.gene85933:intron 50.0%
CACGATTCCACTGCCTAAGA+AGG + chr2.1:1349663-1349682 MS.gene85933:CDS 50.0%
CCTCTTGGTTGTGGTGTAGA+GGG + chr2.1:1352457-1352476 MS.gene85933:CDS 50.0%
CTACTGCACTGCCAGTTAAG+AGG + chr2.1:1348453-1348472 MS.gene85933:CDS 50.0%
CTTCTTAGGCAGTGGAATCG+TGG - chr2.1:1349665-1349684 None:intergenic 50.0%
GGAGTTCCAGTCAAAAGAGG+AGG + chr2.1:1348952-1348971 MS.gene85933:CDS 50.0%
TCAAACGATGGCTAGACACC+GGG + chr2.1:1352685-1352704 MS.gene85933:CDS 50.0%
TCCTCTTGGTTGTGGTGTAG+AGG + chr2.1:1352456-1352475 MS.gene85933:CDS 50.0%
TCTTAGGCAGTGGAATCGTG+GGG - chr2.1:1349663-1349682 None:intergenic 50.0%
TGCAAGTTCTTTCCTCAGCG+AGG - chr2.1:1348639-1348658 None:intergenic 50.0%
TTGTCTCCCTTCTTAGGCAG+TGG - chr2.1:1349673-1349692 None:intergenic 50.0%
TTGTGTGTCCCCGTAGAATG+TGG + chr2.1:1351875-1351894 MS.gene85933:intron 50.0%
! CTAGGGCCAACTTGATTCGT+TGG - chr2.1:1352737-1352756 None:intergenic 50.0%
!! TGCATGCTGTTTCAGGGTTG+TGG + chr2.1:1352054-1352073 MS.gene85933:intron 50.0%
AATTGTTGGCCACAGGGTCC+TGG - chr2.1:1352712-1352731 None:intergenic 55.0%
ACGAACTGGACAGCCAAGTG+GGG + chr2.1:1348294-1348313 MS.gene85933:CDS 55.0%
CACGAACTGGACAGCCAAGT+GGG + chr2.1:1348293-1348312 MS.gene85933:CDS 55.0%
CCCTCTACACCACAACCAAG+AGG - chr2.1:1352460-1352479 None:intergenic 55.0%
TCCTTCCAATCACCCCCACT+TGG - chr2.1:1348310-1348329 None:intergenic 55.0%
AGCTCCCGGTACTTGCCATG+AGG - chr2.1:1351655-1351674 None:intergenic 60.0%
CGAACTGGACAGCCAAGTGG+GGG + chr2.1:1348295-1348314 MS.gene85933:CDS 60.0%
CTCCCCTCATGGCAAGTACC+GGG + chr2.1:1351648-1351667 MS.gene85933:intron 60.0%
CTCCCGGTACTTGCCATGAG+GGG - chr2.1:1351653-1351672 None:intergenic 60.0%
GACAGCCAAGTGGGGGTGAT+TGG + chr2.1:1348302-1348321 MS.gene85933:CDS 60.0%
GCACGAACTGGACAGCCAAG+TGG + chr2.1:1348292-1348311 MS.gene85933:intron 60.0%
GCCAAGTGGGGGTGATTGGA+AGG + chr2.1:1348306-1348325 MS.gene85933:CDS 60.0%
GCTCCCCTCATGGCAAGTAC+CGG + chr2.1:1351647-1351666 MS.gene85933:intron 60.0%
GCTCCCGGTACTTGCCATGA+GGG - chr2.1:1351654-1351673 None:intergenic 60.0%
GATGGCTAGACACCGGGTCC+AGG + chr2.1:1352691-1352710 MS.gene85933:CDS 65.0%
ACACCGGGTCCAGGACCCTG+TGG + chr2.1:1352700-1352719 MS.gene85933:CDS 70.0%
TGGCCACAGGGTCCTGGACC+CGG - chr2.1:1352706-1352725 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 1348018 1352763 1348018 ID=MS.gene85933
chr2.1 mRNA 1348018 1352763 1348018 ID=MS.gene85933.t1;Parent=MS.gene85933
chr2.1 exon 1348018 1348033 1348018 ID=MS.gene85933.t1.exon1;Parent=MS.gene85933.t1
chr2.1 CDS 1348018 1348033 1348018 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
chr2.1 exon 1348293 1348345 1348293 ID=MS.gene85933.t1.exon2;Parent=MS.gene85933.t1
chr2.1 CDS 1348293 1348345 1348293 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
chr2.1 exon 1348443 1349199 1348443 ID=MS.gene85933.t1.exon3;Parent=MS.gene85933.t1
chr2.1 CDS 1348443 1349199 1348443 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
chr2.1 exon 1349536 1350000 1349536 ID=MS.gene85933.t1.exon4;Parent=MS.gene85933.t1
chr2.1 CDS 1349536 1350000 1349536 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
chr2.1 exon 1351327 1351420 1351327 ID=MS.gene85933.t1.exon5;Parent=MS.gene85933.t1
chr2.1 CDS 1351327 1351420 1351327 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
chr2.1 exon 1352432 1352546 1352432 ID=MS.gene85933.t1.exon6;Parent=MS.gene85933.t1
chr2.1 CDS 1352432 1352546 1352432 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
chr2.1 exon 1352650 1352763 1352650 ID=MS.gene85933.t1.exon7;Parent=MS.gene85933.t1
chr2.1 CDS 1352650 1352763 1352650 ID=cds.MS.gene85933.t1;Parent=MS.gene85933.t1
Gene Sequence

>MS.gene85933

ATGGCGACGATGTCAGGTTAGTTTCAATCAGGATAGCATATAATAGGGTTTTGATAAACCGTGAACCTTGATTTTTGCACTTATCCTGTTTCTTTAAATCCTGATAATCATCTTATTTCATTTAAAGAAAAATTGAGACTGTTGTCTTTATCGTTTCCATTTATCGAATGTTTATCCTTCAATTTTTTGCCATCTTAATGCTTTTGGGAATATGCGGTATCCCTGTTGTACCGTTTATCATAATATATATGTTATAGATTATTTGGATTTAACAGCACGAACTGGACAGCCAAGTGGGGGTGATTGGAAGGAAGAAGTAGATCAAAAGGTAATATGCTATTTTCTCATCTTTATTTCTTCAGTGAATAAGGTTGAAAAACAATAAATTCTGTAGTTAAATGATGGATTTTAATGCTTCTGTTTAGAAGATGGATACTACTGCACTGCCAGTTAAGAGGTTATTTGACAGCAACAATTCCTTGGAAAGCAACAATTCCTTGGAATTTTCTGTTAAGAAATCAAAGGTGTCACTATACGATGATCGTGACAAAGATGAAGACGATATTCCTTCGTTGAATATGAATAAAAGGCCAGGCAAATCAGCTGATAATTCATTTTCCTCGCTGAGGAAAGAACTTGCATTGTTGAAAAAATCATTTGAGGAATGCAACGGGAATCAACAAATAGAAGAGTGGCGATTGTGGTCCATAAAGAGAAATATTGAGAAGTGCTGCAAAGAGCTAGAAAATAAGGAGACCCAAGTTAAGGAACTTGAGTCTGAAAAGAATATACTTGATGGTCGAGTAAATGAGCTTGAATCAAAAAAGGGTGAATTTGATGGCCTAGTGAAGAACTTTGAATCAGAAAAGACACATTTCGCGAGCCGACAAAAGGAATTTGAATCAAAAGAAAAGGAATTTGAAAGAAGAGTGAAGGAGTTCCAGTCAAAAGAGGAGGAATTTAAAGGTCGAGTGAAAGTGTTTGAAGCAAAGGAAGAGGAATTCGAAGGAAAAATGCAGCAATTTGAAAACCAAGCTGAGGACAATCTCAAAGCACTCCAGTTAAAAGAGAATCAGATTGAAGTACAAATCAAGGATCCTGAGTCAAAGCTGAATAATTTTGGTGGACAACTAAAGGAGCTTGAGTTGACAGAGAAACAATATGATGAAGAAAAAGAATTTGGTAAATTTTGCTATTCATCTATTTTATTGAAAGAATTTCATTTCAACAACAACTTTTATGGATGCAGTATATCTTAACCAAGTTGAAAAGCTAGTCCAGTTTCTATATCACACACATAATAGTCCTATTTGTAGTTTTGGCTATAATATTAATCAATTAAACACTACTCCAATGATTCACTAAGTTTCTATATTGACATTGAAACTGTTAGTATAATGTTTTTCTCCACATGCTTTCATAGTATATATCTTTTTTAATTCCTATTGTCACCTTCAATTTTTGTTGCTCATGTAAATATGATCAGAGAGCTTGACTATCTGCTATCTAAATATGCAGATACATCTAACATGGATGATCAATTCAGTATCACCATTGATGGAACAAGTGAGGAATTTGGCATTCTAGATAATTTGCGAAAATCATCAGATCCAGCAAAACTTGTTTTGGATATAATACTAAACCCCACGATTCCACTGCCTAAGAAGGGAGACAAAGCTGTGATTATTGATGAAAGCCGCATCTATCTGCTAGAAAAACTGATGATAATCTCACCAGATATTAAATCTTGTGTAAGAGATGAAGCTTTGAAGCTAGCACGTGAATTGAAAGCTAACATGAAAGAAAATACTGAAAATTCTTTGGAGGTTCTCGGATTTCTGATGATTTTGTCGATTTATGGATTGCTTACTTACTTTGATGAAGATGAAGTCTTCAAGCTTTTTGCTTCTGTTGCGTCTGTTGCTGAATACAAGATAGCTGTAAAGCTGTTTAGGACCCTGGGTTTTGTACATAAAGTCTCTGGTATGTTGATTTAGAAATTCTTTCGTCAGTCATCAGAATCTCTCTTATTGTGTCTATGCTTATGTTACATCATATAATTATCAAATCCATATTGCAATTTTGTATCTAATCATGTCTTTTATGTTGTTTTAGATTGGCTTTAAATTTTAGTTGGAAATCAGTGCATACTGTGAACTTAAGAAGTTGATAGAATGTTGATTACATTTGTAGCTCATTTACATTTTTGCATGTTTGATAAAAAATGTATGATAGATTTTTGAGAAAAACTTATGTAGAACATATTATTTGTGTGGTTGTACAAATGTTCCTGAAATAGGACAACTCTTGTTTAAATTTGCCAAAGTCAAATACTTTTAGATGATAAGTAAAAACTATGCAGGATATCCACAAAGATACTAAGATTAATAAGATATTAGATCTAGGTAACTTGGTACAAAATTGAACTCATGCAATTGGCTTCATTTTGTTTCCCCAAACAGTTTTGTCAATTGTTAAGAGAAAATTGACTTCTTGGGTGTTAATGTTTGGAAAATGAAATAGACTTGAAATCTATCTATCTTTTTATGTATCTCTTTCATTTTCTAAATGGTTTGATTAATTTTGAATTATTCAAACCAATTTGTTAGTTTTTTGTTAAGTAGAACTTATTTAGCCTACATTCTAGAAGTTTAAATGATGTGAATTTTTCTTCCACCTTGCAAATTTTGTTAAGAAATCAAGATGAAGAAATTTGATTCAGTTAATTGGCCGACAAGAATGAACAAGTTTTTGCACTAGAAAAATTTATTGTTGAAACCTGCTAATAGGATTTGACTACTAATGCTGGAATCTTAGATTTTTTTTCTTTTGTATTTTAATTTTCAAGGCTTAGTTTTGTTATGTAGAAACATTTTCATGCGTAATTTGTGCAATCATTTTTTTTTTGTAAAGAAACCTTTAAGTTGAATGTGAAAATGACAATCAATTTTAATTTGAATTATCTTGTTATCCACTCTTACAATCCATCTCAAAGAAATAAGAGCTAATATGGGTATTGAGATTGGTTTATGTAGCTCAAAACTTATTTTTTGTTGAATTAAATTAACCATCGATTACAACATCAGTTGAGTTCAACGAAAATAGTTAGAGTTCTGTTATATTATGTGACGAATCAATTTTCAAATTTCGGTTAAGTTGGTTTGAAGCAATTTTAGCTTTTCTCTCCAAAGTGAGGATAAAATAAGAGCAGTTTTAGCTTTTCAGTTTGGCTCACTGAAAAATCAGTACCACTAATTACCTTTTAAATGTTATATTCGTATATATTCATCCTATATACCTTCCTTTCAGAGTCCTGTGATTGGAGAGTTCAATTTCGTACTAGTGATTGGAGTTGGAGAGGTCAATTGTCACCCGAATCACGCACAAGTATTGTCTCCAAAATGTAAGCTTTAGTTTTAATACGGTATCACTGATTTGATATGTAGTTTTTTACTATTAATGTATATCAAAATAACAATTAAATATAGATTGTTGATTGATATGATTCCATAGACTGGCAACATTTCATTAGGTTTTCGGCCTCTTTTTTTATAAGAAAAGAAAAATATATGTATGATAAGGCTCATGCACGGACTTCTAATATGAAAAACGATACATAGTACTATAAAGCTCCCCTCATGGCAAGTACCGGGAGCTCTGTTGTGAATCTATTGCAACAGTCTTATCTTGCATTTGCAAGAGGTTGATTCCACTAATCAAACCTCCACTTCGTAGTCATTTCATATGGAAGAAACTCCATTTGTTGTGTCAAGACTCACCTTCAGACTTCGAATATGATACTGAAGAAACTTATATATAGCACAGATTCAAATAAACACCAGTTTCATGTGTGGGCATTGTGTGTCCCCGTAGAATGTGGATTATGGACTTCTCTTGCTTATTCAATTTAGACCTTTAGGTCTTGAAGGAGGAGAAAGAACCAAGTTTATGTTTTCCATGTGCTATCTTTGTATGCATGAGGCGTGTTTGGTTGGAGATTGCTTTCAATGAGTTTTGAACAAGAACCTGGGTTTTTTGCATGCTGTTTCAGGGTTGTGGTTTTGGCTCTTACGTCACATTCTTCTCAATTTCTTATGTTTTATGGAAAATGGATGTTTATTGGAAAACTAAAGTAATGTTTATAGTATTTATTTTCCTAATGTTGCCAAGAAAATTTCTTAAGGAGGAGAATATCTAAGATGTTATTGAGTAAATTTCGAGTACTCTTAAGCCATGTCTGACGTCCATTCTACTTCCTATTTTTGGCGGGGATAATGAAAACATGTCCAGATGACTTTAGCGAGTTCTTCTTGCATGTCGGTCATATTCTATTATATGAGGGTCGTCCAATGACAATAACATATCAAGAACTCATTTTATTTAATAATTGAGCCACTGTTTTTCATTTTTGTAGCGTGGAGATATTAAAAAGATGTCTTCCTCTTGGTTGTGGTGTAGAGGGATTGCATCATCTTTGGAAGATTGCCCTGCGGTTTGAAGAGAAGATGTTTACAGCTGCAACAAGTCAGGTACATCTCTAATTAGAAGCCTCGTGATTATCAATTTATTATAGTTATCTGTACTAACGCACATGGAAGATTGACACTTTACTGTTGCATTCCCTAATTTCAGTATGATTACAACTGGAAAATAGCTAGTAAGTTGCTTCAAACGATGGCTAGACACCGGGTCCAGGACCCTGTGGCCAACAATTTACCAACGAATCAAGTTGGCCCTAGCCTCTGA
Protein sequence

>MS.gene85933.t1

MATMSARTGQPSGGDWKEEVDQKKMDTTALPVKRLFDSNNSLESNNSLEFSVKKSKVSLYDDRDKDEDDIPSLNMNKRPGKSADNSFSSLRKELALLKKSFEECNGNQQIEEWRLWSIKRNIEKCCKELENKETQVKELESEKNILDGRVNELESKKGEFDGLVKNFESEKTHFASRQKEFESKEKEFERRVKEFQSKEEEFKGRVKVFEAKEEEFEGKMQQFENQAEDNLKALQLKENQIEVQIKDPESKLNNFGGQLKELELTEKQYDEEKEFDTSNMDDQFSITIDGTSEEFGILDNLRKSSDPAKLVLDIILNPTIPLPKKGDKAVIIDESRIYLLEKLMIISPDIKSCVRDEALKLARELKANMKENTENSLEVLGFLMILSIYGLLTYFDEDEVFKLFASVASVAEYKIAVKLFRTLGFVHKVSESCDWRVQFRTSDWSWRGQLSPESRTSIVSKIVEILKRCLPLGCGVEGLHHLWKIALRFEEKMFTAATSQYDYNWKIASKLLQTMARHRVQDPVANNLPTNQVGPSL