Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86062.t1 | ABD32519.1 | 96.9 | 64 | 2 | 0 | 1 | 64 | 9 | 72 | 2.10E-24 | 120.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86062.t1 | Q9SR73 | 81.2 | 64 | 12 | 0 | 1 | 64 | 1 | 64 | 8.4e-22 | 103.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86062.t1 | G7KLE8 | 96.9 | 64 | 2 | 0 | 1 | 64 | 38 | 101 | 1.5e-24 | 120.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051903 | MS.gene86062 | 0.809127 | 2.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86062.t1 | MTR_6g013410 | 96.875 | 64 | 2 | 0 | 1 | 64 | 38 | 101 | 2.29e-39 | 125 |
| MS.gene86062.t1 | MTR_6g013210 | 83.077 | 65 | 10 | 1 | 1 | 64 | 1 | 65 | 4.49e-32 | 105 |
| MS.gene86062.t1 | MTR_4g081180 | 76.923 | 65 | 14 | 1 | 1 | 64 | 150 | 214 | 3.61e-27 | 97.4 |
| MS.gene86062.t1 | MTR_8g089765 | 71.429 | 49 | 13 | 1 | 1 | 48 | 1 | 49 | 1.23e-17 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86062.t1 | AT5G03850 | 81.250 | 64 | 12 | 0 | 1 | 64 | 1 | 64 | 1.45e-32 | 107 |
| MS.gene86062.t1 | AT3G10090 | 81.250 | 64 | 12 | 0 | 1 | 64 | 1 | 64 | 1.45e-32 | 107 |
| MS.gene86062.t1 | AT5G64140 | 81.250 | 64 | 12 | 0 | 1 | 64 | 1 | 64 | 1.76e-32 | 106 |
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGACATCTCCTTCTCTAAT+GGG | 0.349028 | 6.3:+8432820 | None:intergenic |
| AATGACATCTCCTTCTCTAA+TGG | 0.375631 | 6.3:+8432819 | None:intergenic |
| CGCTCAAGTTGAGAAAGTTA+TGG | 0.416411 | 6.3:-8432929 | MS.gene86062:CDS |
| AACCTGAGTGACCTGTCCTC+TGG | 0.457012 | 6.3:+8432894 | None:intergenic |
| GCTCAAGTTGAGAAAGTTAT+GGG | 0.472061 | 6.3:-8432928 | MS.gene86062:CDS |
| GAGAAAGTTATGGGTCGTAC+TGG | 0.491245 | 6.3:-8432919 | MS.gene86062:CDS |
| CTTCTCTCTCAGACTCAAGT+AGG | 0.493446 | 6.3:+8432794 | None:intergenic |
| TCGTACTGGATCCAGAGGAC+AGG | 0.601747 | 6.3:-8432905 | MS.gene86062:CDS |
| ATCCAGAGGACAGGTCACTC+AGG | 0.602839 | 6.3:-8432896 | MS.gene86062:CDS |
| GTGAAGGGCCCCATTAGAGA+AGG | 0.605680 | 6.3:-8432829 | MS.gene86062:CDS |
| ATGAGAATCGCTACATCATG+AGG | 0.605973 | 6.3:-8432855 | MS.gene86062:CDS |
| TTCTCTCTCAGACTCAAGTA+GGG | 0.606054 | 6.3:+8432795 | None:intergenic |
| CTACATCATGAGGAATGTGA+AGG | 0.633338 | 6.3:-8432845 | MS.gene86062:CDS |
| TGACATCTCCTTCTCTAATG+GGG | 0.633980 | 6.3:+8432821 | None:intergenic |
| TACATCATGAGGAATGTGAA+GGG | 0.640443 | 6.3:-8432844 | MS.gene86062:CDS |
| ATGGGTCGTACTGGATCCAG+AGG | 0.663896 | 6.3:-8432910 | MS.gene86062:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGACATCTCCTTCTCTAA+TGG | + | chr6.3:8432910-8432929 | None:intergenic | 35.0% | |
| ATGACATCTCCTTCTCTAAT+GGG | + | chr6.3:8432909-8432928 | None:intergenic | 35.0% | |
| TACATCATGAGGAATGTGAA+GGG | - | chr6.3:8432882-8432901 | MS.gene86062:CDS | 35.0% | |
| !! | GCTCAAGTTGAGAAAGTTAT+GGG | - | chr6.3:8432798-8432817 | MS.gene86062:CDS | 35.0% |
| ATGAGAATCGCTACATCATG+AGG | - | chr6.3:8432871-8432890 | MS.gene86062:CDS | 40.0% | |
| CTACATCATGAGGAATGTGA+AGG | - | chr6.3:8432881-8432900 | MS.gene86062:CDS | 40.0% | |
| TGACATCTCCTTCTCTAATG+GGG | + | chr6.3:8432908-8432927 | None:intergenic | 40.0% | |
| TTCTCTCTCAGACTCAAGTA+GGG | + | chr6.3:8432934-8432953 | None:intergenic | 40.0% | |
| !! | CGCTCAAGTTGAGAAAGTTA+TGG | - | chr6.3:8432797-8432816 | MS.gene86062:CDS | 40.0% |
| CTTCTCTCTCAGACTCAAGT+AGG | + | chr6.3:8432935-8432954 | None:intergenic | 45.0% | |
| GAGAAAGTTATGGGTCGTAC+TGG | - | chr6.3:8432807-8432826 | MS.gene86062:CDS | 45.0% | |
| AACCTGAGTGACCTGTCCTC+TGG | + | chr6.3:8432835-8432854 | None:intergenic | 55.0% | |
| ATCCAGAGGACAGGTCACTC+AGG | - | chr6.3:8432830-8432849 | MS.gene86062:CDS | 55.0% | |
| ATGGGTCGTACTGGATCCAG+AGG | - | chr6.3:8432816-8432835 | MS.gene86062:CDS | 55.0% | |
| GTGAAGGGCCCCATTAGAGA+AGG | - | chr6.3:8432897-8432916 | MS.gene86062:CDS | 55.0% | |
| TCGTACTGGATCCAGAGGAC+AGG | - | chr6.3:8432821-8432840 | MS.gene86062:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 8432777 | 8432971 | 8432777 | ID=MS.gene86062 |
| chr6.3 | mRNA | 8432777 | 8432971 | 8432777 | ID=MS.gene86062.t1;Parent=MS.gene86062 |
| chr6.3 | exon | 8432777 | 8432971 | 8432777 | ID=MS.gene86062.t1.exon1;Parent=MS.gene86062.t1 |
| chr6.3 | CDS | 8432777 | 8432971 | 8432777 | ID=cds.MS.gene86062.t1;Parent=MS.gene86062.t1 |
| Gene Sequence |
| Protein sequence |
