AlfalfaGEDB | MS.gene86280

M. sativa cultivar XinJiangDaYe / MS.gene86280

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF13041.9 PF13812.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86280.t1	XP_024630982.1	95.6	592	19	2	1	592	1	585	5.20E-287	996.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86280.t1	Q8VYD6	59.7	559	220	1	20	578	21	574	1.7e-164	580.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86280.t1	A0A396JEZ4	95.6	592	19	2	1	592	1	585	3.8e-287	996.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055254	MS.gene86280	0.801947	7.08E-49	-1.69E-46
MS.gene055397	MS.gene86280	0.808013	3.81E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86280.t1	MTR_1g009650	95.593	590	19	2	1	590	1	583	0.0	1117
MS.gene86280.t1	MTR_8g107050	25.234	321	215	8	56	374	170	467	2.66e-20	95.5
MS.gene86280.t1	MTR_8g107050	24.118	340	213	11	59	396	313	609	8.44e-17	84.7
MS.gene86280.t1	MTR_8g107050	23.580	352	239	10	80	429	124	447	1.95e-12	70.5
MS.gene86280.t1	MTR_8g107050	25.234	321	215	8	56	374	170	467	2.71e-20	95.5
MS.gene86280.t1	MTR_8g107050	24.118	340	213	11	59	396	313	609	8.42e-17	84.3
MS.gene86280.t1	MTR_8g107050	23.580	352	239	10	80	429	124	447	1.81e-12	70.9
MS.gene86280.t1	MTR_3g105900	24.352	386	258	9	27	408	216	571	3.81e-20	95.5
MS.gene86280.t1	MTR_3g105900	25.085	295	200	6	80	374	830	1103	1.98e-18	90.1
MS.gene86280.t1	MTR_3g105900	23.058	412	245	15	48	416	336	718	1.61e-16	84.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86280.t1	AT5G10690	59.325	563	224	1	16	578	17	574	0.0	689
MS.gene86280.t1	AT5G10690	59.325	563	224	1	16	578	17	574	0.0	689
MS.gene86280.t1	AT1G64580	25.688	327	197	8	80	376	183	493	1.07e-22	102
MS.gene86280.t1	AT1G09900	25.210	357	236	11	19	371	180	509	1.29e-22	102
MS.gene86280.t1	AT1G09900	25.098	255	169	8	82	333	345	580	3.51e-11	66.6
MS.gene86280.t1	AT1G09680	27.221	349	214	10	79	411	238	562	5.75e-22	100
MS.gene86280.t1	AT1G12775	28.517	263	172	6	73	335	255	501	5.75e-22	100
MS.gene86280.t1	AT1G62914	26.885	305	193	9	64	365	239	516	5.34e-21	97.1
MS.gene86280.t1	AT1G63400	26.667	345	211	12	64	393	246	563	5.46e-21	97.4
MS.gene86280.t1	AT1G62670	27.507	349	207	12	64	395	242	561	8.93e-21	97.1

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Find 130 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCTCAACCGGTTCAGA+AGG	0.579685	1.3:+10544073	None:intergenic
AAAGCAACACGGCCACTACA+AGG	0.595532	1.3:-10544037	MS.gene86280:CDS
AAATACAGAAACAGGTTGGT+TGG	0.498251	1.3:-10545219	MS.gene86280:CDS
AAGATTGTTCCTGAAGTATC+TGG	0.404575	1.3:+10545091	None:intergenic
AAGGTGGTCAGCTTCTTCTT+GGG	0.465281	1.3:+10545060	None:intergenic
AATGATGGAGAGGGTTTGAG+TGG	0.462781	1.3:+10548405	None:intergenic
AATGGCCTCCTTGCCTGTTA+TGG	0.294395	1.3:-10546879	MS.gene86280:CDS
AATTTACAATGATAACCATT+GGG	0.527781	1.3:+10543490	None:intergenic
ACAAGGTACCATAAAGCTGA+AGG	0.615792	1.3:-10544020	MS.gene86280:CDS
ACACCGTGAAGACTGCAATC+AGG	0.518070	1.3:-10543924	MS.gene86280:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTTTAAATTTGTGGGTG+TGG	-	chr1.3:10546312-10546331	MS.gene86280:intron	30.0%
AAAGTAATGTACACTTAGCA+AGG	+	chr1.3:10544650-10544669	None:intergenic	30.0%
ACTTACTACTGGACAATATT+TGG	+	chr1.3:10547242-10547261	None:intergenic	30.0%
ATGTTGCCATGCAATTATTT+GGG	-	chr1.3:10545255-10545274	MS.gene86280:intron	30.0%
ATTATTATGGTATGTCTGGA+TGG	+	chr1.3:10544290-10544309	None:intergenic	30.0%
ATTTATAAAGGGTTTAGGCA+AGG	-	chr1.3:10544399-10544418	MS.gene86280:CDS	30.0%
CATATTTCAGTAGTAGAGAT+TGG	-	chr1.3:10545043-10545062	MS.gene86280:CDS	30.0%
CATTAAAAAGTCATGTTGAC+TGG	-	chr1.3:10544850-10544869	MS.gene86280:intron	30.0%
CGTATTGTCAATTTCTGATT+CGG	-	chr1.3:10548146-10548165	MS.gene86280:CDS	30.0%
CTTCAATTTCATTCAGAATC+TGG	+	chr1.3:10543553-10543572	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	10543362	10548459	10543362	ID=MS.gene86280
chr1.3	mRNA	10543362	10548459	10543362	ID=MS.gene86280.t1;Parent=MS.gene86280
chr1.3	exon	10548085	10548459	10548085	ID=MS.gene86280.t1.exon1;Parent=MS.gene86280.t1
chr1.3	CDS	10548085	10548459	10548085	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10547280	10547412	10547280	ID=MS.gene86280.t1.exon2;Parent=MS.gene86280.t1
chr1.3	CDS	10547280	10547412	10547280	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10546821	10546918	10546821	ID=MS.gene86280.t1.exon3;Parent=MS.gene86280.t1
chr1.3	CDS	10546821	10546918	10546821	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10546535	10546696	10546535	ID=MS.gene86280.t1.exon4;Parent=MS.gene86280.t1
chr1.3	CDS	10546535	10546696	10546535	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10546380	10546448	10546380	ID=MS.gene86280.t1.exon5;Parent=MS.gene86280.t1
chr1.3	CDS	10546380	10546448	10546380	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10545923	10546058	10545923	ID=MS.gene86280.t1.exon6;Parent=MS.gene86280.t1
chr1.3	CDS	10545923	10546058	10545923	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10545030	10545262	10545030	ID=MS.gene86280.t1.exon7;Parent=MS.gene86280.t1
chr1.3	CDS	10545030	10545262	10545030	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10544863	10544940	10544863	ID=MS.gene86280.t1.exon8;Parent=MS.gene86280.t1
chr1.3	CDS	10544863	10544940	10544863	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10544387	10544455	10544387	ID=MS.gene86280.t1.exon9;Parent=MS.gene86280.t1
chr1.3	CDS	10544387	10544455	10544387	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10543925	10544101	10543925	ID=MS.gene86280.t1.exon10;Parent=MS.gene86280.t1
chr1.3	CDS	10543925	10544101	10543925	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1
chr1.3	exon	10543362	10543613	10543362	ID=MS.gene86280.t1.exon11;Parent=MS.gene86280.t1
chr1.3	CDS	10543362	10543613	10543362	ID=cds.MS.gene86280.t1;Parent=MS.gene86280.t1

Gene Sequence

>MS.gene86280

ATGCTTCTAACTCACGCTGCTTTCTCTTCAATCCACTCAAACCCTCTCCATCATTCTTCATACATTCCCATTTCTTCGTGTTCTTCATCTTCTCCGATACGACGTCGTTTATTTACCACTCCTCGCAAAACGAATCTGAAACACCTCACATCCCGCATAGTTCAACTCACTCGCCGAAAACAGCTTCACCAGATTCTGAATGAAATTGAAGTCGCGAAGAAACGTTTTGGAAAATTGAATACGATTGTGATGAATGCGGTTATGGAAGCTTGTGTTCGTTGCGGTGATATCGATTCAGCTCTTAACATTTTTGATGAGATGAAGAATCCTGAAGCTTGTGGTGTTGATACTGTCACCTATGCCACGCTTTTGAAGGTATTATTCTGATTATAGAAATTATTGAATGATTAATAAGGTATGTTTAGATAGAGTAGATTTTTCATTTTAAATAGGGAATTATAGAGAGATTATTTGTAATGAAATGGATAAATGATTAAAGTGTAACTTTTTTGTGATCATAATGTGCTATTGAAACTAATTTTACTGAAAAGTGATAAGTGTTGTTGTGTGAGATTATATGTTGTGAGTATGACAATGTTGTAATGGATTAGTGATTGTGCAATAGGTAGAGTTACCGAGAAACCAAGGAAAATTGTAGGCTAATCATTAATAATGACTTAGATTAAATAGTCTATCATTATTCATGATTTATGTTAGGATACGCTAATGTTGTTTGATTCAAGTAGCTAGCTAGCTTGATTTAGTGGAACAAGATTTTATTTTTGGGTTATTGAAAGGAAATATTCAAAGTTAACCTCGAAACTTTCAATTACCCATTATTAATCATTTCATTATAATCATTTTAGAAATTTCATAATTAAAATGAAAAATATTAACAGTCCCTTTCCTTTAGCCACAAGTCCCTCCATCCAGACATACCATAATAATCTTACAGATTACTTTTTATCTAATAATAATTCATTCTGGTATGGTTTTTAATTATATGTTCTATTTAAGCTTGATTGGATTTTATTTAAATTTATAAAGGGTTTAGGCAAGGCGCATAGATTTGATGAAGCTTTTCGATTGCTTGAATCTGTTGAAAATGGTACTGCCGTGGGAAGTCCAAAGTTGTCAGCACCATTGGTCTTTGGTTTACTAAATGCCTTAGCAGAAGCAGGTGAGTTGGATATTTCAGTCTCCCTGATACCTATCCCTGTCGGATCTTTGCTGTTTGTCTCATCATCTATTGTATTAAATGTTTTTCATTTCTGGGGATTTCTGGCCTTGCTAAGTGTACATTACTTTTTATTTTGTTATGATCTTTTTTTTTCCTGATCAATTTTTGTTTTTGCATTGCATGGTCAGACATGTAAAAATGTATATGTTAGCTGAAGATATAAGAAGTTTTTGTGTGATTGATATTTTGTTTGCTTTCAATGTTTAAGGTGTTAGATGTATGTTCTGGTTTTAGTCTATGATAAAATTCATTAAAAAGTCATGTTGACTGGTGATGTTTTACTTGCAATGCTATAGTGCAGGAGACCTGCGTCGTGCAAATGGCCTCCTTGCCTGTTATGGTTCCGTGCTTCGTGAAGGCGAACATTTTTCAGTCTCAGTATACAACCTTCTAATAAAGGTTTACTTCTGAACCTTAGGGAGAAAATTTCATTATGAATAGCATATTTCAGTAGTAGAGATTGGAAATATTTGTCTGATCCGAGCAATAATTTGAATGGTTATTTTTTTTCCCACTAATTTAGGGGCATATAAACTCAGGCTGTCCTCATACTGCAATAAACATGCTCAATGAAATTTTACATCAAGGAATAATGCCTGACAGGCTCACATATAACACACTAATTTTAGCATGTGTTCAGAGTGAAAAGTTGGATGTTGCCATGCAATTATTTGGGGAAATGAAGGTAATAACTTTTATTTGAAATATAACTTTTAATAGTTTTGTGGTAGATAAATCTGGACCTTCTAAATTCCAACCTTTATATTATAGGAAAAAGCGCAGGAATTCATCCATGATGATCTGTTTCCAGATATCGTTACATACACAACATTGCTCAAGGTCAGATTTCTCAAATTTACTCACTTGTAATATTAGTTTGTTGTAGTACGAAACCATTAAAGTTTAAATCAAGTTTTGTTTTCTTCTGTATAACGTTTTGATTGTTGGAGTCCGGAGTTATGTTCACTCATTTCAGTTTTCTTGAAATTGCTTTTATTTTAATGATTATTTAAGTTCTCATAGTTTAAGCAGTCATGTATGAAAATGGCATGCCTTTAACATTTATTTTAAACAAATTTAGTTATGTCCTCTATTATAATTTTATCAGGGGTTTAGGAGGGATATGTGGCAGCATCTCTCAGTTTTTCTCTTTATTTGCAGGGTTTGGGGCAGGCAAGGGATCTCGTTTCAGTTATGCAAATAGTGCTGGAGATGAAATCATGTTCTGAGTTATACATTGATAGAACTGCTTACACAGCAATAGTTGATGCTTTACTAAAATGTGGCTCAGTTAAAGGTATGCTTTCATTTGATTCCGGTTTTCTTTAGTTGCTTGGAGATAGTTTCAGGAATAATTAAAAGGATAAATGTTTATGGTAGTTGGAATGAGATATTCCACCTTTTTTTTTTTGGATGGGTGGAGTAAGATATTCCTAACAAGTGCTAATAATCAAAATATGTATGAGATTAACGAGTTCTACCTGTCTCTTGAATTGAAGGGTGAAACATTATTATAATGCTGATTTGCATTTTACATTCTTCTAAGTTGAGTGAAGATTATGAATGTCGTATTTTATTATAAATTTCACCAATTATTTAGTTAGAAATGGATAATAGTTTAACTTTTGGCCGTGTTTGCTGAAAGGTTATGTCTCATAAGGACTGATAAAACTTTCAGTTAAGTGGCCAGTTGAACCAAGTACCAAGTTAAAACTTTAAATTTGTGGGTGTGGCACAAAGAGAACTATACTCAATTAAACATAGTAAAGGAATGGAAATCTACTGTTATGAGTTTCAATTACTAGTTCATTTTCTAATGAAGCTTGAATGGATACTATTAGTATTCTTCTTTAATAATCACTCTATTAGTGCTTTCAGACAATCCTTTTGTTTCATTAACTAATTGATGAATTTGTATTTTGTTCATCATATAATTGAATTATCTTGGGGTATATAGGTGCTCTGTGTATTTTTGGAGAAATACAGAAACAGGTTGGTTGGAATCCAGAGTTGAGACCGAAGCCTCATCTCTATCTTTCCATGATGCGTGCCTTTGCTTCTAGAGGAGATTATGATCTGGTTAAAAACTTGCATAAGCGGATTTTGCCAGATACTTCAGGAACAATCTTACCAGTTTCCCAAGAAGAAGCTGACCACCTTCTAATGGAAGCAGCCCTGAACGCTGGCCAGGTATTATTAATATTTCTGTTTCTAATTTTCAACATTTTGATTGGGACGGGGAAGCTAATAATTCACTTGCTCTTGACATTTTATCCCAGGTTGATGTCGCTACTAAAACTCTTATGTATATTGTTTCAAGATGGAAGGGCATATCTTGGACGAGTCATGGAGGCATGGTGTGTAGTTAGTTTTTTCATGAATGTTGTTTTATATACATCCATTCAGTAGACTTTTAAGTATTAGCATTGATTTATATATCTGGTTCGTTTCTTATCACATTAATTTTTAGGATCAACTGGTACTTCAGTTGATATTTTTGGTGTCCTTTTTACAATGCAAAGATTCCTTTGCAGAGAATCGGTTTTTAGCTTGATTTATTTTTTTTATTTTTTTGACTTGGTGTTTCTTTTCTAATTCTAAGTATAATATTACCGTGGATTACATTTAATACTGTTGCCAAATATTGTCCAGTAGTAAGTTTCTGAACAAGCTGTTAGGCATTTAATTGTTTCATTTCCCTTAAAATGCTAAGGCTGGCAGCAAGTCAGGTTCTTAATCATTTGCCTGTGCTTCTGTAATTTAGGTTGCTTCTCGTATTGAGGCCTTATTGGGATTCTCAGCATCTGTATTCAATCCTTTCTTACTTCCTCAGGTACTTAACATATATAATATATTCACTGACATCCTTTTCTCTAGGTGGCATTGAGCTATATTAAGCTAAATAACAAGTATGCTCTATTTGTTTGTAAATGCATTTTAACCTAGTTTGTCTAAAACTACTGTCTTGCAGGCTTTCATGCTTTTATTTATATATTCATAATTCACAGACCCTCTGACTCCAGCCTTTGGCTGTTTTTTCTGCATTGCATTTTTGGTAGTTTCTTTTTTCTTCTTTGAGAATTTGTATATAGTCTTATTATGCAACCTCATGGTTCAGGTTTCACCTTCTGAACCGGTTGAGGGTTTCATGATGTCATTTAAAGCAACACGGCCACTACAAGGTACCATAAAGCTGAAGGATGTGGTGTCACGTTTCTTTAATGATGCAGTAGTTCCTATTGTAGATGAGTGGGGCAGTTGTACAGGGCTGTTACACCGTGAAGACTGCAATCAGGTATGTTTTGCGAGATTGCCGTTTGATGAAATAGTGTTCTACTATAAAGATATGCATTCTATAGTCTTTAAGTCAGACATGGAGAGAAGCTAATACTAATGCATGTACTTTCTGTCTTGGGCTATTACAGACCCGTTTGTTCATGCTTTGTCAAAGAAATCAACATTTTTTTAATTACAAAAATGTTTTAATATGATTTAGAACAATACATAGACATCGATACAAATTAAAAAATAATTCTTCATTCGACGTATTGTCAATTTCTGATTCGGCCTCTAACCATCTAATACTATGATGAATTCATTCTGCAGTTGGATGCTCCACTTTCAACAATGATGAGAAGTCCTCCTCCCAGTGTTACAGCTTCAACATCTGTTGGTCATGTAATTGATTTAATTTTGGAGAAGGGTTACCCAATGGTTATCATTGTAAATTGCACTGACTCGTATGCTACTTCATACGGCTGTAGAGCAGTTGGAGTTTTTACATTAGAACAATTGAATAGGCTCATCAAACCTGCACCTGAAGTGAAAGAGACAGACCTTTCACTCTGCAGACCATAG

Protein sequence

>MS.gene86280.t1

MLLTHAAFSSIHSNPLHHSSYIPISSCSSSSPIRRRLFTTPRKTNLKHLTSRIVQLTRRKQLHQILNEIEVAKKRFGKLNTIVMNAVMEACVRCGDIDSALNIFDEMKNPEACGVDTVTYATLLKGLGKAHRFDEAFRLLESVENGTAVGSPKLSAPLVFGLLNALAEAGDLRRANGLLACYGSVLREGEHFSVSVYNLLIKGHINSGCPHTAINMLNEILHQGIMPDRLTYNTLILACVQSEKLDVAMQLFGEMKEKAQEFIHDDLFPDIVTYTTLLKGLGQARDLVSVMQIVLEMKSCSELYIDRTAYTAIVDALLKCGSVKGALCIFGEIQKQVGWNPELRPKPHLYLSMMRAFASRGDYDLVKNLHKRILPDTSGTILPVSQEEADHLLMEAALNAGQVDVATKTLMYIVSRWKGISWTSHGGMVASRIEALLGFSASVFNPFLLPQVSPSEPVEGFMMSFKATRPLQGTIKLKDVVSRFFNDAVVPIVDEWGSCTGLLHREDCNQLDAPLSTMMRSPPPSVTASTSVGHVIDLILEKGYPMVIIVNCTDSYATSYGCRAVGVFTLEQLNRLIKPAPEVKETDLSLCRP