AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86304

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86304.t1 AT2G24440 64.706 85 27 2 124 207 100 182 2.63e-34 120
MS.gene86304.t1 AT4G31360 44.516 155 61 3 52 204 51 182 1.48e-28 106

Find 64 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCGGCGGCGCCGTCGCC+GGG 0.240695 1.3:+10276436 MS.gene86304:CDS
CCATACATTCATTAGCCTTT+TGG 0.252653 1.3:+10278986 MS.gene86304:CDS
GAAGGCGGCGGCGCCGTCGC+CGG 0.261617 1.3:+10276435 MS.gene86304:CDS
GGTTCATCATATTTGTCTTC+AGG 0.276320 1.3:-10276730 None:intergenic
GACGGCGCCGCCGCCTTCGC+CGG 0.333123 1.3:-10276430 None:intergenic
GCTGACAGTGAAGGTGCTTC+TGG 0.354259 1.3:+10276616 MS.gene86304:CDS
TGAATGTATGGCCACCTTGC+TGG 0.361738 1.3:-10278974 None:intergenic
CGAGACAAGCCTTGCACCTT+CGG 0.369026 1.3:-10276503 None:intergenic
AGATCCTTCATTGGCTTGAA+TGG 0.378234 1.3:-10279401 None:intergenic
CGCTTCTCCGGCGAAGGCGG+CGG 0.382542 1.3:+10276423 MS.gene86304:CDS
ACAAATATGATGAACCTAAC+AGG 0.395796 1.3:+10276737 MS.gene86304:CDS
GCTTCTGGTACTACTGGTTC+TGG 0.397333 1.3:+10276631 MS.gene86304:CDS
GCATCAGAACCTCCACCTTC+AGG 0.404405 1.3:-10276697 None:intergenic
CGCCGCCTTCGCCGGAGAAG+CGG 0.409883 1.3:-10276422 None:intergenic
GAAAGCTGAACCGAAGGATA+AGG 0.411247 1.3:+10276582 MS.gene86304:CDS
CCAAAAGGCTAATGAATGTA+TGG 0.417197 1.3:-10278986 None:intergenic
GGCATGTCAAGATCCTTCAT+TGG 0.424164 1.3:-10279410 None:intergenic
GCTTTCTTTCCCTTATCCTT+CGG 0.424678 1.3:-10276592 None:intergenic
TTCTTCTGCTTCTTCTTCGC+CGG 0.429999 1.3:-10276550 None:intergenic
TACTGAGGTGAAGGATGGTC+TGG 0.453806 1.3:+10277944 MS.gene86304:CDS
AACAATATCTGCGATGACCT+TGG 0.457435 1.3:-10279432 None:intergenic
GAAGGTGCTTCTGGTACTAC+TGG 0.470059 1.3:+10276625 MS.gene86304:CDS
ACGACGATAACAGTCCTGTT+AGG 0.472960 1.3:-10276751 None:intergenic
GGTACTACTGGTTCTGGTGG+TGG 0.473554 1.3:+10276637 MS.gene86304:CDS
ACAATATCTGCGATGACCTT+GGG 0.478658 1.3:-10279431 None:intergenic
GTTGCTGATTTATCAAATGC+TGG 0.479820 1.3:+10279452 MS.gene86304:CDS
TGGTGCTGACCCGAAGGGTA+AGG 0.492398 1.3:+10276657 MS.gene86304:CDS
AAGCCTTGCACCTTCGGAGT+TGG 0.492453 1.3:-10276497 None:intergenic
CAAGGCTAAAACCGCTTCTC+CGG 0.494433 1.3:+10276411 MS.gene86304:CDS
AAAGCTGAACCGAAGGATAA+GGG 0.495239 1.3:+10276583 MS.gene86304:CDS
ATTTCAAAACAACCTCTCCT+TGG 0.500246 1.3:-10278948 None:intergenic
TAAAACCGCTTCTCCGGCGA+AGG 0.530878 1.3:+10276417 MS.gene86304:CDS
CTGTTATCGTCGTTGAGCAT+TGG 0.551171 1.3:+10276761 MS.gene86304:CDS
TCGTCCATTCAAGCCAATGA+AGG 0.551201 1.3:+10279397 MS.gene86304:CDS
GAGGAGAGCTACTGAGGTGA+AGG 0.553058 1.3:+10277935 MS.gene86304:CDS
TGGTGGTGGTGCTGACCCGA+AGG 0.556830 1.3:+10276651 MS.gene86304:CDS
GGTGCTGACCCGAAGGGTAA+GGG 0.558148 1.3:+10276658 MS.gene86304:CDS
GGCGAAGAAGAAGCAGAAGA+AGG 0.558274 1.3:+10276552 MS.gene86304:CDS
GTTCCAACTCCGAAGGTGCA+AGG 0.564313 1.3:+10276494 MS.gene86304:CDS
GCGGCGGCGCCGTCGCCGGG+AGG 0.565820 1.3:+10276439 MS.gene86304:CDS
GAAGAAGAAAGCTGAACCGA+AGG 0.566958 1.3:+10276576 MS.gene86304:CDS
AACCGCTTCTCCGGCGAAGG+CGG 0.575146 1.3:+10276420 MS.gene86304:CDS
CTCAATGCAAATCATTCTTG+AGG 0.583853 1.3:+10277916 MS.gene86304:CDS
GGTGAAACTGAACCCTGAAA+AGG 0.589350 1.3:+10277992 MS.gene86304:CDS
TGACATTCAGAAGAAATCTC+CGG 0.589803 1.3:+10276531 MS.gene86304:CDS
TGAGGTGAAGGATGGTCTGG+AGG 0.590927 1.3:+10277947 MS.gene86304:CDS
GAGGTGAAGGATGGTCTGGA+GGG 0.591530 1.3:+10277948 MS.gene86304:CDS
AGAGCTACTGAGGTGAAGGA+TGG 0.600555 1.3:+10277939 MS.gene86304:CDS
AAGAAAGCCGCTGACAGTGA+AGG 0.603856 1.3:+10276607 MS.gene86304:CDS
AGAAATGGCACCAAAACGCA+AGG 0.626423 1.3:+10276393 None:intergenic
AAACAAGCCGCTGTACCTGA+AGG 0.636179 1.3:+10276682 MS.gene86304:CDS
GCCGCTGTACCTGAAGGTGG+AGG 0.642345 1.3:+10276688 MS.gene86304:CDS
CAAGCCGCTGTACCTGAAGG+TGG 0.643554 1.3:+10276685 MS.gene86304:CDS
ATTCTTGAGGAGAGCTACTG+AGG 0.643723 1.3:+10277929 MS.gene86304:CDS
GGAACGAAGTGTTCTTCCAG+AGG 0.646097 1.3:-10276476 None:intergenic
ACACTTCGTTCCAACTCCGA+AGG 0.650374 1.3:+10276487 MS.gene86304:CDS
TCTGGTACTACTGGTTCTGG+TGG 0.655303 1.3:+10276634 MS.gene86304:CDS
GAAGGATCTTGACATGCCCA+AGG 0.657771 1.3:+10279415 MS.gene86304:CDS
TAGTCAAGCTATTGATGTGG+TGG 0.665049 1.3:+10277971 MS.gene86304:CDS
GGTGGTGGTGCTGACCCGAA+GGG 0.687297 1.3:+10276652 MS.gene86304:CDS
ACCTCCACCTTCAGGTACAG+CGG 0.699979 1.3:-10276689 None:intergenic
TTTGAAATACGCCAGCAAGG+TGG 0.702724 1.3:+10278963 MS.gene86304:CDS
GGGTAGTCAAGCTATTGATG+TGG 0.707014 1.3:+10277968 MS.gene86304:CDS
AGAAGCACCTTCACTGTCAG+CGG 0.707449 1.3:-10276614 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTTTAAGGAAAAAAT+TGG - chr1.3:10278925-10278944 None:intergenic 10.0%
!! ATAAATGGAATATATTGAAA+TGG + chr1.3:10277232-10277251 MS.gene86304:intron 15.0%
!! GAAAAATGACTAAAAAATAA+TGG - chr1.3:10279130-10279149 None:intergenic 15.0%
!! TCAAAAAGAAATTGTAAAAA+AGG - chr1.3:10278806-10278825 None:intergenic 15.0%
!! TTGAAAACAAAATGTTTAAA+AGG - chr1.3:10278747-10278766 None:intergenic 15.0%
!!! AATTTTTCAATCCATTTTTT+CGG - chr1.3:10277175-10277194 None:intergenic 15.0%
!!! CAATTTCTTTATTAGTTTAA+AGG + chr1.3:10279268-10279287 MS.gene86304:intron 15.0%
!! ATGGAATATAGATAATGAAA+TGG + chr1.3:10277331-10277350 MS.gene86304:intron 20.0%
!! ATTGAATAGAGTGTAATAAT+TGG + chr1.3:10277516-10277535 MS.gene86304:intron 20.0%
!! GTTGATTAAAGTGTAATAAA+TGG + chr1.3:10277217-10277236 MS.gene86304:intron 20.0%
!! TAAAGTGGTAAAATAATCAA+TGG - chr1.3:10278147-10278166 None:intergenic 20.0%
!! TAGTAAAAATAATTTCACTG+AGG - chr1.3:10277789-10277808 None:intergenic 20.0%
!! TTATAACACATAATGTAAAG+TGG - chr1.3:10278162-10278181 None:intergenic 20.0%
!!! AAAGAAGCAAATTATTTTTC+AGG - chr1.3:10277664-10277683 None:intergenic 20.0%
!!! AATATTTTTCGTCATCATTA+CGG - chr1.3:10276996-10277015 None:intergenic 20.0%
!!! AATTTTTTATGTGGATAGTT+TGG + chr1.3:10277428-10277447 MS.gene86304:intron 20.0%
!!! ACAAATAGAAAAAAGTACAA+AGG - chr1.3:10279069-10279088 None:intergenic 20.0%
!!! CAACATGAACATTTTTTAAT+TGG + chr1.3:10279030-10279049 MS.gene86304:intron 20.0%
!!! GAAGTTTTTTTTGGATATAT+TGG + chr1.3:10279104-10279123 MS.gene86304:intron 20.0%
!!! GCTATATTTGAAGTTTTTTT+TGG + chr1.3:10279095-10279114 MS.gene86304:intron 20.0%
! AACATTGTGAAGATTTCAAA+GGG - chr1.3:10278393-10278412 None:intergenic 25.0%
! ATAATTGGAATGTAATGAAC+TGG + chr1.3:10277531-10277550 MS.gene86304:intron 25.0%
! ATGAAATACTGCTTAATTTC+AGG + chr1.3:10279367-10279386 MS.gene86304:intron 25.0%
! TATATCAAACCCTAATTTCT+TGG + chr1.3:10276896-10276915 MS.gene86304:intron 25.0%
! TGTGTTATAATACAATCATG+TGG + chr1.3:10278172-10278191 MS.gene86304:intron 25.0%
!! CAAAATGTTTAAAAGGCTTT+TGG - chr1.3:10278740-10278759 None:intergenic 25.0%
!! GATTGAAATCTTTTGTTGAT+AGG + chr1.3:10277254-10277273 MS.gene86304:intron 25.0%
!! GTTGATGTTTGGAATATAAA+TGG + chr1.3:10277312-10277331 MS.gene86304:intron 25.0%
!!! CTGATTATAATGTTGCTTTT+TGG + chr1.3:10277742-10277761 MS.gene86304:intron 25.0%
!!! TCTTCTTATTTGTTTTTCAG+TGG + chr1.3:10278260-10278279 MS.gene86304:intron 25.0%
!!! TTTGCTTCTTTTGATTAATG+TGG + chr1.3:10277673-10277692 MS.gene86304:intron 25.0%
!!! TTTGGTGGTAATTTTTTATG+TGG + chr1.3:10277419-10277438 MS.gene86304:intron 25.0%
!!! TTTGTTGATAGGTTATGTTT+TGG + chr1.3:10277265-10277284 MS.gene86304:intron 25.0%
AATGAAATCATCCATTGAAG+AGG - chr1.3:10279208-10279227 None:intergenic 30.0%
ACAAATATGATGAACCTAAC+AGG + chr1.3:10276737-10276756 MS.gene86304:CDS 30.0%
ACATTGTGAAGATTTCAAAG+GGG - chr1.3:10278392-10278411 None:intergenic 30.0%
CAACATTGTGAAGATTTCAA+AGG - chr1.3:10278394-10278413 None:intergenic 30.0%
CGGTTAATTCCAAGAAATTA+GGG - chr1.3:10276908-10276927 None:intergenic 30.0%
GACATAAACAAAATAGCAGA+AGG - chr1.3:10278055-10278074 None:intergenic 30.0%
TCCTTAAAAATTTACAGCCA+AGG + chr1.3:10278931-10278950 MS.gene86304:intron 30.0%
TCGGTTAATTCCAAGAAATT+AGG - chr1.3:10276909-10276928 None:intergenic 30.0%
TTCTTGGAATTAACCGAAAA+TGG + chr1.3:10276912-10276931 MS.gene86304:intron 30.0%
! AAAGCTTAGAATACCTTTTC+AGG - chr1.3:10278008-10278027 None:intergenic 30.0%
! AACAGAGTATTAAGACAACA+AGG - chr1.3:10278876-10278895 None:intergenic 30.0%
! AAGCTTAGAATACCTTTTCA+GGG - chr1.3:10278007-10278026 None:intergenic 30.0%
! AATGACTATCTAAAGCAATG+AGG + chr1.3:10277636-10277655 MS.gene86304:intron 30.0%
! ATGATTTGCATTGAGTACTA+AGG - chr1.3:10277911-10277930 None:intergenic 30.0%
! GATTAGTTAGAAGTAGAACT+AGG + chr1.3:10278204-10278223 MS.gene86304:intron 30.0%
! GCTTTTGATAGATTCTTGTT+TGG + chr1.3:10277401-10277420 MS.gene86304:intron 30.0%
! TATACAGAATCCACCATTTT+CGG - chr1.3:10276928-10276947 None:intergenic 30.0%
! TCCTTGGCTGTAAATTTTTA+AGG - chr1.3:10278935-10278954 None:intergenic 30.0%
! TTAGCTTATGTGTTGATGTT+TGG + chr1.3:10277301-10277320 MS.gene86304:intron 30.0%
! TTTCTTTTTGAACAACGAGA+AGG + chr1.3:10278815-10278834 MS.gene86304:intron 30.0%
! TTTGATAGATTCTTGTTTGG+TGG + chr1.3:10277404-10277423 MS.gene86304:intron 30.0%
! TTTTTATGTGGATAGTTTGG+TGG + chr1.3:10277431-10277450 MS.gene86304:intron 30.0%
!! CTTTGAAATCTTCACAATGT+TGG + chr1.3:10278392-10278411 MS.gene86304:intron 30.0%
!! TCTTATTTGTTTTTCAGTGG+AGG + chr1.3:10278263-10278282 MS.gene86304:intron 30.0%
!!! TTTCAATCCATTTTTTCGGT+TGG - chr1.3:10277171-10277190 None:intergenic 30.0%
AAAAATTTACAGCCAAGGAG+AGG + chr1.3:10278936-10278955 MS.gene86304:intron 35.0%
AAAGCAAGAAAAGGGCAAAA+CGG - chr1.3:10276813-10276832 None:intergenic 35.0%
ACCCTTACCTAGTATTTGTT+TGG - chr1.3:10278474-10278493 None:intergenic 35.0%
AGGCTGAGATCTTAATACAA+TGG - chr1.3:10279188-10279207 None:intergenic 35.0%
ATATGCATGTGTAATGTGTG+TGG - chr1.3:10278578-10278597 None:intergenic 35.0%
ATCTGTACGAAAGCAAGAAA+AGG - chr1.3:10276822-10276841 None:intergenic 35.0%
ATTTCAAAACAACCTCTCCT+TGG - chr1.3:10278951-10278970 None:intergenic 35.0%
ATTTCCCCCAAACAAATACT+AGG + chr1.3:10278464-10278483 MS.gene86304:intron 35.0%
CATTGTGAAGATTTCAAAGG+GGG - chr1.3:10278391-10278410 None:intergenic 35.0%
CCAAAAGGCTAATGAATGTA+TGG - chr1.3:10278989-10279008 None:intergenic 35.0%
CCCAAACAAATACTAGGTAA+GGG + chr1.3:10278470-10278489 MS.gene86304:intron 35.0%
CCCTTACCTAGTATTTGTTT+GGG - chr1.3:10278473-10278492 None:intergenic 35.0%
CCTTACCTAGTATTTGTTTG+GGG - chr1.3:10278472-10278491 None:intergenic 35.0%
CTCAATGCAAATCATTCTTG+AGG + chr1.3:10277916-10277935 MS.gene86304:CDS 35.0%
CTTACCTAGTATTTGTTTGG+GGG - chr1.3:10278471-10278490 None:intergenic 35.0%
GAAATCTTCACAATGTTGGA+GGG + chr1.3:10278396-10278415 MS.gene86304:intron 35.0%
GGTTCATCATATTTGTCTTC+AGG - chr1.3:10276733-10276752 None:intergenic 35.0%
GTATCATTTAGATTGCCAGT+AGG - chr1.3:10279297-10279316 None:intergenic 35.0%
TAGAAGTAGAACTAGGAAGA+GGG + chr1.3:10278211-10278230 MS.gene86304:intron 35.0%
TAGTATTTGTTTGGGGGAAA+TGG - chr1.3:10278465-10278484 None:intergenic 35.0%
TCAAACTTCTGCTTCACTTT+AGG + chr1.3:10278629-10278648 MS.gene86304:intron 35.0%
TCTGTACGAAAGCAAGAAAA+GGG - chr1.3:10276821-10276840 None:intergenic 35.0%
TGAAATCTTCACAATGTTGG+AGG + chr1.3:10278395-10278414 MS.gene86304:intron 35.0%
TGACATTCAGAAGAAATCTC+CGG + chr1.3:10276531-10276550 MS.gene86304:CDS 35.0%
TTAGAAGTAGAACTAGGAAG+AGG + chr1.3:10278210-10278229 MS.gene86304:intron 35.0%
TTATCAACTGTGACGATTGT+TGG + chr1.3:10278774-10278793 MS.gene86304:intron 35.0%
TTGGAATTAACCGAAAATGG+TGG + chr1.3:10276915-10276934 MS.gene86304:intron 35.0%
! TTTGGAACCAACCGAAAAAA+TGG + chr1.3:10277161-10277180 MS.gene86304:intron 35.0%
!! AAAGGCTTTTGGTTGAGTTT+AGG - chr1.3:10278729-10278748 None:intergenic 35.0%
!! GTTGATAGGTTATGTTTTGG+TGG + chr1.3:10277268-10277287 MS.gene86304:intron 35.0%
!! GTTGCTGATTTATCAAATGC+TGG + chr1.3:10279452-10279471 MS.gene86304:CDS 35.0%
!! TTAGTTTAAAGGCATCCTAC+TGG + chr1.3:10279279-10279298 MS.gene86304:intron 35.0%
!!! CCATACATTCATTAGCCTTT+TGG + chr1.3:10278986-10279005 MS.gene86304:CDS 35.0%
AAAGCTGAACCGAAGGATAA+GGG + chr1.3:10276583-10276602 MS.gene86304:CDS 40.0%
AACAATATCTGCGATGACCT+TGG - chr1.3:10279435-10279454 None:intergenic 40.0%
ACAATATCTGCGATGACCTT+GGG - chr1.3:10279434-10279453 None:intergenic 40.0%
AGGGTTTGTGTACAACTTAC+TGG + chr1.3:10278489-10278508 MS.gene86304:intron 40.0%
CCCCAAACAAATACTAGGTA+AGG + chr1.3:10278469-10278488 MS.gene86304:intron 40.0%
GCTTTCTTTCCCTTATCCTT+CGG - chr1.3:10276595-10276614 None:intergenic 40.0%
GGAAAAAATCTTCGTTCCTC+TGG + chr1.3:10276460-10276479 MS.gene86304:CDS 40.0%
GGGTTTGTGTACAACTTACT+GGG + chr1.3:10278490-10278509 MS.gene86304:intron 40.0%
GGTTTGTGTACAACTTACTG+GGG + chr1.3:10278491-10278510 MS.gene86304:intron 40.0%
GTACAGATGCACGATAATGA+CGG + chr1.3:10276834-10276853 MS.gene86304:intron 40.0%
GTTTGTGTACAACTTACTGG+GGG + chr1.3:10278492-10278511 MS.gene86304:intron 40.0%
TAAGATCTCAGCCTCTTCAA+TGG + chr1.3:10279194-10279213 MS.gene86304:intron 40.0%
TAGTCAAGCTATTGATGTGG+TGG + chr1.3:10277971-10277990 MS.gene86304:CDS 40.0%
! AAAATAGCAGAAGGCATGAG+TGG - chr1.3:10278046-10278065 None:intergenic 40.0%
! CGGTTGGTTCCAAAAAAGTA+GGG - chr1.3:10277155-10277174 None:intergenic 40.0%
! TCGGTTGGTTCCAAAAAAGT+AGG - chr1.3:10277156-10277175 None:intergenic 40.0%
! TGGTTGAGTTTAGGTTTGCA+GGG - chr1.3:10278720-10278739 None:intergenic 40.0%
! TTGGTTGAGTTTAGGTTTGC+AGG - chr1.3:10278721-10278740 None:intergenic 40.0%
!! AGATCCTTCATTGGCTTGAA+TGG - chr1.3:10279404-10279423 None:intergenic 40.0%
!! CATGTTGATAACAAGAGAGC+AGG - chr1.3:10279017-10279036 None:intergenic 40.0%
!!! GAACGAAGATTTTTTCCTCC+CGG - chr1.3:10276457-10276476 None:intergenic 40.0%
!!! GAGACGAAACCCTACTTTTT+TGG + chr1.3:10277143-10277162 MS.gene86304:intron 40.0%
!!! TGTTTTGAAATACGCCAGCA+AGG + chr1.3:10278960-10278979 MS.gene86304:CDS 40.0%
ACGACGATAACAGTCCTGTT+AGG - chr1.3:10276754-10276773 None:intergenic 45.0%
ACTGTGACGATTGTTGGTCA+TGG + chr1.3:10278780-10278799 MS.gene86304:intron 45.0%
AGAGAGCAGGACTTACCAAA+AGG - chr1.3:10279004-10279023 None:intergenic 45.0%
AGCAGAGAGAAAGTAGGAAG+AGG - chr1.3:10278678-10278697 None:intergenic 45.0%
AGTAGAACTAGGAAGAGGGA+AGG + chr1.3:10278215-10278234 MS.gene86304:intron 45.0%
AGTGGAAGCAGAGAGAAAGT+AGG - chr1.3:10278684-10278703 None:intergenic 45.0%
ATTCTTGAGGAGAGCTACTG+AGG + chr1.3:10277929-10277948 MS.gene86304:CDS 45.0%
CAGGGTGAGAGTCAAAATAG+TGG - chr1.3:10278702-10278721 None:intergenic 45.0%
CTGTTATCGTCGTTGAGCAT+TGG + chr1.3:10276761-10276780 MS.gene86304:CDS 45.0%
GAAAGCTGAACCGAAGGATA+AGG + chr1.3:10276582-10276601 MS.gene86304:CDS 45.0%
GAAGAAGAAAGCTGAACCGA+AGG + chr1.3:10276576-10276595 MS.gene86304:CDS 45.0%
GCAGAGAGAAAGTAGGAAGA+GGG - chr1.3:10278677-10278696 None:intergenic 45.0%
GGCATGTCAAGATCCTTCAT+TGG - chr1.3:10279413-10279432 None:intergenic 45.0%
GGGTAGTCAAGCTATTGATG+TGG + chr1.3:10277968-10277987 MS.gene86304:CDS 45.0%
GGTGAAACTGAACCCTGAAA+AGG + chr1.3:10277992-10278011 MS.gene86304:CDS 45.0%
TCGTCCATTCAAGCCAATGA+AGG + chr1.3:10279397-10279416 MS.gene86304:CDS 45.0%
TGTTGGAGGGAGCTAATGTT+TGG + chr1.3:10278409-10278428 MS.gene86304:intron 45.0%
TTCTTCTGCTTCTTCTTCGC+CGG - chr1.3:10276553-10276572 None:intergenic 45.0%
! TTTGAAATACGCCAGCAAGG+TGG + chr1.3:10278963-10278982 MS.gene86304:CDS 45.0%
!! CTTGTTTTCCCTTACCCTTC+GGG - chr1.3:10276669-10276688 None:intergenic 45.0%
!! GCTTGTTTTCCCTTACCCTT+CGG - chr1.3:10276670-10276689 None:intergenic 45.0%
!!! TATCAAATTTTTTTTTTTTT+TGG - chr1.3:10277482-10277501 None:intergenic 5.0%
AAACAAGCCGCTGTACCTGA+AGG + chr1.3:10276682-10276701 MS.gene86304:CDS 50.0%
AACTAGGAAGAGGGAAGGAG+AGG + chr1.3:10278220-10278239 MS.gene86304:intron 50.0%
AAGAAAGCCGCTGACAGTGA+AGG + chr1.3:10276607-10276626 MS.gene86304:CDS 50.0%
ACACTTCGTTCCAACTCCGA+AGG + chr1.3:10276487-10276506 MS.gene86304:CDS 50.0%
ACTAGGAAGAGGGAAGGAGA+GGG + chr1.3:10278221-10278240 MS.gene86304:intron 50.0%
AGAGCTACTGAGGTGAAGGA+TGG + chr1.3:10277939-10277958 MS.gene86304:CDS 50.0%
CAAGGCTAAAACCGCTTCTC+CGG + chr1.3:10276411-10276430 MS.gene86304:CDS 50.0%
GAAGGATCTTGACATGCCCA+AGG + chr1.3:10279415-10279434 MS.gene86304:CDS 50.0%
GGCGAAGAAGAAGCAGAAGA+AGG + chr1.3:10276552-10276571 MS.gene86304:CDS 50.0%
TACTGAGGTGAAGGATGGTC+TGG + chr1.3:10277944-10277963 MS.gene86304:CDS 50.0%
TGAATGTATGGCCACCTTGC+TGG - chr1.3:10278977-10278996 None:intergenic 50.0%
! GGAACGAAGTGTTCTTCCAG+AGG - chr1.3:10276479-10276498 None:intergenic 50.0%
!! AGAAGCACCTTCACTGTCAG+CGG - chr1.3:10276617-10276636 None:intergenic 50.0%
!! GAAGGTGCTTCTGGTACTAC+TGG + chr1.3:10276625-10276644 MS.gene86304:CDS 50.0%
!! GCTTCTGGTACTACTGGTTC+TGG + chr1.3:10276631-10276650 MS.gene86304:CDS 50.0%
!! TCTGGTACTACTGGTTCTGG+TGG + chr1.3:10276634-10276653 MS.gene86304:CDS 50.0%
!!! AGATTTTTTCCTCCCGGCGA+CGG - chr1.3:10276451-10276470 None:intergenic 50.0%
!!! GCGGTTTTAGCCTTGCGTTT+TGG - chr1.3:10276406-10276425 None:intergenic 50.0%
AAGCCTTGCACCTTCGGAGT+TGG - chr1.3:10276500-10276519 None:intergenic 55.0%
ACCTCCACCTTCAGGTACAG+CGG - chr1.3:10276692-10276711 None:intergenic 55.0%
CGAGACAAGCCTTGCACCTT+CGG - chr1.3:10276506-10276525 None:intergenic 55.0%
CTAGGAAGAGGGAAGGAGAG+GGG + chr1.3:10278222-10278241 MS.gene86304:intron 55.0%
GAGGAGAGCTACTGAGGTGA+AGG + chr1.3:10277935-10277954 MS.gene86304:CDS 55.0%
GAGGTGAAGGATGGTCTGGA+GGG + chr1.3:10277948-10277967 MS.gene86304:CDS 55.0%
GCATCAGAACCTCCACCTTC+AGG - chr1.3:10276700-10276719 None:intergenic 55.0%
TAAAACCGCTTCTCCGGCGA+AGG + chr1.3:10276417-10276436 MS.gene86304:CDS 55.0%
TAGGAAGAGGGAAGGAGAGG+GGG + chr1.3:10278223-10278242 MS.gene86304:intron 55.0%
TGAGGTGAAGGATGGTCTGG+AGG + chr1.3:10277947-10277966 MS.gene86304:CDS 55.0%
! GTTCCAACTCCGAAGGTGCA+AGG + chr1.3:10276494-10276513 MS.gene86304:CDS 55.0%
!! GCTGACAGTGAAGGTGCTTC+TGG + chr1.3:10276616-10276635 MS.gene86304:CDS 55.0%
!! GGTACTACTGGTTCTGGTGG+TGG + chr1.3:10276637-10276656 MS.gene86304:CDS 55.0%
AGGAAGAGGGAAGGAGAGGG+GGG + chr1.3:10278224-10278243 MS.gene86304:intron 60.0%
CAAGCCGCTGTACCTGAAGG+TGG + chr1.3:10276685-10276704 MS.gene86304:CDS 60.0%
!! GGTGCTGACCCGAAGGGTAA+GGG + chr1.3:10276658-10276677 MS.gene86304:CDS 60.0%
!! TGGTGCTGACCCGAAGGGTA+AGG + chr1.3:10276657-10276676 MS.gene86304:CDS 60.0%
AACCGCTTCTCCGGCGAAGG+CGG + chr1.3:10276420-10276439 MS.gene86304:CDS 65.0%
AGAGGGAAGGAGAGGGGGGT+TGG + chr1.3:10278228-10278247 MS.gene86304:intron 65.0%
GCCGCTGTACCTGAAGGTGG+AGG + chr1.3:10276688-10276707 MS.gene86304:CDS 65.0%
!! GGTGGTGGTGCTGACCCGAA+GGG + chr1.3:10276652-10276671 MS.gene86304:CDS 65.0%
!! TGGTGGTGGTGCTGACCCGA+AGG + chr1.3:10276651-10276670 MS.gene86304:CDS 65.0%
CGCCGCCTTCGCCGGAGAAG+CGG - chr1.3:10276425-10276444 None:intergenic 75.0%
CGCTTCTCCGGCGAAGGCGG+CGG + chr1.3:10276423-10276442 MS.gene86304:CDS 75.0%
! AAGGCGGCGGCGCCGTCGCC+GGG + chr1.3:10276436-10276455 MS.gene86304:CDS 85.0%
! GAAGGCGGCGGCGCCGTCGC+CGG + chr1.3:10276435-10276454 MS.gene86304:CDS 85.0%
! GACGGCGCCGCCGCCTTCGC+CGG - chr1.3:10276433-10276452 None:intergenic 85.0%
! GCGGCGGCGCCGTCGCCGGG+AGG + chr1.3:10276439-10276458 MS.gene86304:CDS 95.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 10276397 10279478 10276397 ID=MS.gene86304
chr1.3 mRNA 10276397 10279478 10276397 ID=MS.gene86304.t1;Parent=MS.gene86304
chr1.3 exon 10276397 10276782 10276397 ID=MS.gene86304.t1.exon1;Parent=MS.gene86304.t1
chr1.3 CDS 10276397 10276782 10276397 ID=cds.MS.gene86304.t1;Parent=MS.gene86304.t1
chr1.3 exon 10277914 10278013 10277914 ID=MS.gene86304.t1.exon2;Parent=MS.gene86304.t1
chr1.3 CDS 10277914 10278013 10277914 ID=cds.MS.gene86304.t1;Parent=MS.gene86304.t1
chr1.3 exon 10278948 10279007 10278948 ID=MS.gene86304.t1.exon3;Parent=MS.gene86304.t1
chr1.3 CDS 10278948 10279007 10278948 ID=cds.MS.gene86304.t1;Parent=MS.gene86304.t1
chr1.3 exon 10279389 10279478 10279389 ID=MS.gene86304.t1.exon4;Parent=MS.gene86304.t1
chr1.3 CDS 10279389 10279478 10279389 ID=cds.MS.gene86304.t1;Parent=MS.gene86304.t1
Gene Sequence

>MS.gene86304

ATGGCACCAAAACGCAAGGCTAAAACCGCTTCTCCGGCGAAGGCGGCGGCGCCGTCGCCGGGAGGAAAAAATCTTCGTTCCTCTGGAAGAACACTTCGTTCCAACTCCGAAGGTGCAAGGCTTGTCTCGTTAGATGACATTCAGAAGAAATCTCCGGCGAAGAAGAAGCAGAAGAAGGAGAAGAAGAAAGCTGAACCGAAGGATAAGGGAAAGAAAGCCGCTGACAGTGAAGGTGCTTCTGGTACTACTGGTTCTGGTGGTGGTGCTGACCCGAAGGGTAAGGGAAAACAAGCCGCTGTACCTGAAGGTGGAGGTTCTGATGCTGCTGATAAACCTGAAGACAAATATGATGAACCTAACAGGACTGTTATCGTCGTTGAGCATTGGTATTTCACTCTTTCGCTTTTTTTTTACCGTTTTGCCCTTTTCTTGCTTTCGTACAGATGCACGATAATGACGGAGAAATTATTATTCGATTTTGTTTCGTTAATGCAAATTTTATATCAAACCCTAATTTCTTGGAATTAACCGAAAATGGTGGATTCTGTATAAGCTATTTTTAATCGTTTTCTCTTTTCTTGCTTTCGTAATAATGTCCGTAATGATGACGAAAAATATTATGCGCTTTTTCAGTTTTTTTATTTTTATTTTATTTTTATGCATGTGTTTTGTGTTTGTCTTTATCTTTATCTGCTTATTTTGTTTTTATATTAGCTTAATCAATATGAAAACGATTTATTTTTTGTGAGACGAAACCCTACTTTTTTGGAACCAACCGAAAAAATGGATTGAAAAATTTGTTGATTAAACTGAATCTTTTGTTGATTAAAGTGTAATAAATGGAATATATTGAAATGGATTGAAATCTTTTGTTGATAGGTTATGTTTTGGTGGTAAATCTTGATTAGCTTATGTGTTGATGTTTGGAATATAAATGGAATATAGATAATGAAATGGATTGAATAAATTGTTGATTAAAGTGAATCTTTTGTTGATAAATTGAAGCTTTTGATAGATTCTTGTTTGGTGGTAATTTTTTATGTGGATAGTTTGGTGGTTAAATTTGATAGTATGTGTTTGTACCAAAAAAAAAAAAAATTTGATAGTATGTGTTTATATATTGAATAGAGTGTAATAATTGGAATGTAATGAACTGGATTGAAAAAGAGTGTCATTTTTTTGATGATTAATTGATGCAGATTGTTGAAATATTAATGATAATATTTCTTGATTGATTATAATGACTATCTAAAGCAATGAGGAGCCTGAAAAATAATTTGCTTCTTTTGATTAATGTGGAATTTTGAGATAATTACAAATAATCTTTTTAACAAGATTCTTGAATCTGATTATAATGTTGCTTTTTGGTTCTTGTTGCTTCATTATATACCTCAGTGAAATTATTTTTACTATTATTGATTTCTCTCTCTAACATTGTAACTGTTTTCTCAATTGAGTGACTGATTATTGTTTTACTATTATTGATTTCTCAATTTTTTTGTCTTGATTTTCCTTAGTACTCAATGCAAATCATTCTTGAGGAGAGCTACTGAGGTGAAGGATGGTCTGGAGGGTAGTCAAGCTATTGATGTGGTGGTGAAACTGAACCCTGAAAAGGTATTCTAAGCTTTATTTATCATTTCTATCCACTCATGCCTTCTGCTATTTTGTTTATGTCAATGATATCAAATAGATATACATGTTTTTCAAAAAAACATTGATTATAATACAATCAATGTTTATTCTGCCATTGATTATTTTACCACTTTACATTATGTGTTATAATACAATCATGTGGTGTTTGAATGATTAGTTAGAAGTAGAACTAGGAAGAGGGAAGGAGAGGGGGGTTGGTCAATAGAATCTTCTTATTTGTTTTTCAGTGGAGGTGTGATTTTAATAATGAAAACACTAATTCAAAATTTACTCTAAGTTTATGTATTTAAAATATTTATGCAATTCATGTTCACTGTTTTATTCATCTCTGATCTCCCCCTTTGAAATCTTCACAATGTTGGAGGGAGCTAATGTTTGGTGAAGAGAGATTTTTCATATCTCTCTATATCCATTTCCCCCAAACAAATACTAGGTAAGGGTTTGTGTACAACTTACTGGGGGAGTTTTTGAAAATAAGTTGACAACTTATTGACATCTTATATATTATTTTTAAAAGTTCTTCCACACACATTACACATGCATATTTATATGATATAAGCTCTCAAATAAGCTCTTTCAAACTTCTGCTTCACTTTAGGTGACATCTCTACTAAACTCTCACCCTCTTCCTACTTTCTCTCTGCTTCCACTATTTTGACTCTCACCCTGCAAACCTAAACTCAACCAAAAGCCTTTTAAACATTTTGTTTTCAATGATAGCTTATCAACTGTGACGATTGTTGGTCATGGCCTTTTTTACAATTTCTTTTTGAACAACGAGAAGGACTATTGAATTCATATGCTATATTATTTAACTAGTCCTTGTTGTCTTAATACTCTGTTTTTTATGTCAACAATACTTGATTGATCCAATTTTTTCCTTAAAAATTTACAGCCAAGGAGAGGTTGTTTTGAAATACGCCAGCAAGGTGGCCATACATTCATTAGCCTTTTGGTAAGTCCTGCTCTCTTGTTATCAACATGAACATTTTTTAATTGGCAAGCTAAATCAACCTTTGTACTTTTTTCTATTTGTTCATATGCTATATTTGAAGTTTTTTTTGGATATATTGGCCATTATTTTTTAGTCATTTTTCTTGCTCAAGAAAGTATTAAGATTTTTTAGATTGTACCATTGTATTAAGATCTCAGCCTCTTCAATGGATGATTTCATTCACATGAAAATATTTCTTTTCTGTATGTTTTAAAGAACTTCAATTTCTTTATTAGTTTAAAGGCATCCTACTGGCAATCTAAATGATACATGCATACATTTAATTATATTTTTATGATATTCTGTTTTAATACTAACTTATGAAATACTGCTTAATTTCAGGACATGAGTCGTCCATTCAAGCCAATGAAGGATCTTGACATGCCCAAGGTCATCGCAGATATTGTTGCTGATTTATCAAATGCTGGTTGA
Protein sequence

>MS.gene86304.t1

MAPKRKAKTASPAKAAAPSPGGKNLRSSGRTLRSNSEGARLVSLDDIQKKSPAKKKQKKEKKKAEPKDKGKKAADSEGASGTTGSGGGADPKGKGKQAAVPEGGGSDAADKPEDKYDEPNRTVIVVEHCTQCKSFLRRATEVKDGLEGSQAIDVVVKLNPEKPRRGCFEIRQQGGHTFISLLDMSRPFKPMKDLDMPKVIADIVADLSNAG