Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86949.t1 | XP_003597940.1 | 98.6 | 210 | 2 | 1 | 1 | 210 | 1 | 209 | 1.60E-100 | 375.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86949.t1 | G7IIA4 | 98.6 | 210 | 2 | 1 | 1 | 210 | 1 | 209 | 1.1e-100 | 375.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene86949 | 0.801568 | 8.47E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene86949 | 0.812069 | 5.10E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene86949 | 0.812239 | 4.69E-51 | -1.69E-46 |
| MS.gene050330 | MS.gene86949 | -0.803428 | 3.50E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene86949 | -0.806736 | 7.12E-50 | -1.69E-46 |
| MS.gene055217 | MS.gene86949 | 0.810393 | 1.18E-50 | -1.69E-46 |
| MS.gene061114 | MS.gene86949 | -0.823412 | 1.40E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86949.t1 | MTR_2g104230 | 98.571 | 210 | 2 | 1 | 1 | 210 | 1 | 209 | 1.21e-155 | 429 |
| MS.gene86949.t1 | MTR_4g009760 | 60.181 | 221 | 64 | 10 | 1 | 210 | 1 | 208 | 2.13e-81 | 241 |
| MS.gene86949.t1 | MTR_4g116200 | 45.912 | 159 | 70 | 3 | 1 | 158 | 1 | 144 | 2.68e-34 | 122 |
| MS.gene86949.t1 | MTR_2g450720 | 38.378 | 185 | 86 | 6 | 1 | 165 | 1 | 177 | 2.08e-29 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86949.t1 | AT1G15760 | 44.444 | 225 | 79 | 8 | 3 | 209 | 5 | 201 | 9.92e-58 | 181 |
| MS.gene86949.t1 | AT1G80520 | 47.489 | 219 | 77 | 8 | 1 | 209 | 1 | 191 | 9.52e-51 | 163 |
| MS.gene86949.t1 | AT2G12462 | 29.231 | 260 | 121 | 7 | 1 | 209 | 1 | 248 | 4.74e-26 | 101 |
Find 42 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAGGCCTAATGGTGGATT+TGG | 0.285518 | 2.3:-709793 | MS.gene86949:CDS |
| GCTTTGTCTGGTGATCTTGA+TGG | 0.316091 | 2.3:-709655 | MS.gene86949:CDS |
| GAGAATTTCTAATCTGTGTT+TGG | 0.325351 | 2.3:+709957 | None:intergenic |
| TAAGTGGGATACCATGTTTA+AGG | 0.337355 | 2.3:-709512 | MS.gene86949:CDS |
| GAGAAAATGTTTCTCACAAA+TGG | 0.339341 | 2.3:-709712 | MS.gene86949:CDS |
| CCATCTCTTGTATATGAGTA+TGG | 0.354378 | 2.3:-710066 | MS.gene86949:CDS |
| TACTGAAGAATCAGCACTTA+TGG | 0.357191 | 2.3:-709824 | MS.gene86949:CDS |
| AGTACTTAGCTAACTATGTT+AGG | 0.383843 | 2.3:-709858 | MS.gene86949:CDS |
| TTCTAATCTGTGTTTGGCTA+TGG | 0.404684 | 2.3:+709963 | None:intergenic |
| ATAATCATGATGATGGTTAT+TGG | 0.406535 | 2.3:-709555 | MS.gene86949:CDS |
| CTAGGCCTAATGGTGGATTT+GGG | 0.437057 | 2.3:-709792 | MS.gene86949:CDS |
| ACAGTAGCTCCTGCTTTGTC+TGG | 0.454059 | 2.3:-709667 | MS.gene86949:CDS |
| CACTTATGGTGATGCCAACT+AGG | 0.468970 | 2.3:-709810 | MS.gene86949:CDS |
| GAAGAGGAACACACAATTTG+TGG | 0.477404 | 2.3:-709746 | MS.gene86949:CDS |
| CCATGCTTTGTAGAAACTCA+TGG | 0.497469 | 2.3:+709992 | None:intergenic |
| TTAACTCAACAGCCATGGAT+TGG | 0.500177 | 2.3:-710119 | None:intergenic |
| TTTCCAAAAGGAGCAAAAGA+TGG | 0.506781 | 2.3:-709608 | MS.gene86949:CDS |
| AATCCACCATTAGGCCTAGT+TGG | 0.510240 | 2.3:+709796 | None:intergenic |
| ATGGTGGATTTGGGAGAAGA+TGG | 0.518326 | 2.3:-709783 | MS.gene86949:CDS |
| CCATGAGTTTCTACAAAGCA+TGG | 0.527981 | 2.3:-709992 | MS.gene86949:CDS |
| CAAAAGATGGATGGAGATGA+TGG | 0.529408 | 2.3:-709595 | MS.gene86949:CDS |
| TACAAGAGATGGCTCAAGGT+TGG | 0.536104 | 2.3:+710077 | None:intergenic |
| CTGCTGTTGAAGAGATTAAG+TGG | 0.538740 | 2.3:-709528 | MS.gene86949:CDS |
| CTTCTCCCAAATCCACCATT+AGG | 0.539738 | 2.3:+709787 | None:intergenic |
| GTGATGCCAACTAGGCCTAA+TGG | 0.547391 | 2.3:-709802 | MS.gene86949:CDS |
| GATGGAGATGATGGTGGTGA+TGG | 0.550508 | 2.3:-709586 | MS.gene86949:CDS |
| TGTTGTGTATCATTTCCAAA+AGG | 0.557274 | 2.3:-709620 | MS.gene86949:CDS |
| CAAAAGGAGCAAAAGATGGA+TGG | 0.561894 | 2.3:-709604 | MS.gene86949:CDS |
| AATAATGATAATCATGATGA+TGG | 0.562055 | 2.3:-709562 | MS.gene86949:CDS |
| CCATACTCATATACAAGAGA+TGG | 0.573633 | 2.3:+710066 | None:intergenic |
| TCAAGATCACCAGACAAAGC+AGG | 0.592387 | 2.3:+709658 | None:intergenic |
| CATGAGTTTCTACAAAGCAT+GGG | 0.601787 | 2.3:-709991 | MS.gene86949:CDS |
| GGAAGAGTGCTATAATGAAG+AGG | 0.605141 | 2.3:-709762 | MS.gene86949:CDS |
| AAATGTTTCTCACAAATGGA+AGG | 0.609404 | 2.3:-709708 | MS.gene86949:CDS |
| CATATACAAGAGATGGCTCA+AGG | 0.614307 | 2.3:+710073 | None:intergenic |
| AATTTGTAGCTGCAATCAAG+AGG | 0.614506 | 2.3:-709888 | MS.gene86949:CDS |
| TAGCCATGAAAACCAATCCA+TGG | 0.623251 | 2.3:+710107 | None:intergenic |
| TGCTGTTGAAGAGATTAAGT+GGG | 0.633418 | 2.3:-709527 | MS.gene86949:CDS |
| TGGCTTTAGATCCTTAAACA+TGG | 0.634954 | 2.3:+709501 | None:intergenic |
| ATGCCAACTAGGCCTAATGG+TGG | 0.656564 | 2.3:-709799 | MS.gene86949:CDS |
| GAGGAACACACAATTTGTGG+TGG | 0.682058 | 2.3:-709743 | MS.gene86949:CDS |
| AAGATGGATGGAGATGATGG+TGG | 0.707845 | 2.3:-709592 | MS.gene86949:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATGATAATCATGATGA+TGG | - | chr2.3:710038-710057 | MS.gene86949:CDS | 20.0% |
| !!! | ATAATCATGATGATGGTTAT+TGG | - | chr2.3:710045-710064 | MS.gene86949:CDS | 25.0% |
| AAATGTTTCTCACAAATGGA+AGG | - | chr2.3:709892-709911 | MS.gene86949:CDS | 30.0% | |
| AGTACTTAGCTAACTATGTT+AGG | - | chr2.3:709742-709761 | MS.gene86949:CDS | 30.0% | |
| GAGAAAATGTTTCTCACAAA+TGG | - | chr2.3:709888-709907 | MS.gene86949:CDS | 30.0% | |
| GAGAATTTCTAATCTGTGTT+TGG | + | chr2.3:709646-709665 | None:intergenic | 30.0% | |
| TGTTGTGTATCATTTCCAAA+AGG | - | chr2.3:709980-709999 | MS.gene86949:CDS | 30.0% | |
| !! | TTTTGGAAATGATACACAAC+AGG | + | chr2.3:709981-710000 | None:intergenic | 30.0% |
| AATTTGTAGCTGCAATCAAG+AGG | - | chr2.3:709712-709731 | MS.gene86949:CDS | 35.0% | |
| CATGAGTTTCTACAAAGCAT+GGG | - | chr2.3:709609-709628 | MS.gene86949:CDS | 35.0% | |
| CCATACTCATATACAAGAGA+TGG | + | chr2.3:709537-709556 | None:intergenic | 35.0% | |
| CCATCTCTTGTATATGAGTA+TGG | - | chr2.3:709534-709553 | MS.gene86949:CDS | 35.0% | |
| TAAGTGGGATACCATGTTTA+AGG | - | chr2.3:710088-710107 | MS.gene86949:CDS | 35.0% | |
| TACTGAAGAATCAGCACTTA+TGG | - | chr2.3:709776-709795 | MS.gene86949:CDS | 35.0% | |
| TGCTGTTGAAGAGATTAAGT+GGG | - | chr2.3:710073-710092 | MS.gene86949:CDS | 35.0% | |
| TTCTAATCTGTGTTTGGCTA+TGG | + | chr2.3:709640-709659 | None:intergenic | 35.0% | |
| TTTCCAAAAGGAGCAAAAGA+TGG | - | chr2.3:709992-710011 | MS.gene86949:CDS | 35.0% | |
| ! | ACAAATTTTGCAACTGTGTG+TGG | + | chr2.3:709699-709718 | None:intergenic | 35.0% |
| !! | TGGCTTTAGATCCTTAAACA+TGG | + | chr2.3:710102-710121 | None:intergenic | 35.0% |
| CAAAAGATGGATGGAGATGA+TGG | - | chr2.3:710005-710024 | MS.gene86949:CDS | 40.0% | |
| CAAAAGGAGCAAAAGATGGA+TGG | - | chr2.3:709996-710015 | MS.gene86949:CDS | 40.0% | |
| CATATACAAGAGATGGCTCA+AGG | + | chr2.3:709530-709549 | None:intergenic | 40.0% | |
| CCATGAGTTTCTACAAAGCA+TGG | - | chr2.3:709608-709627 | MS.gene86949:CDS | 40.0% | |
| CCATGCTTTGTAGAAACTCA+TGG | + | chr2.3:709611-709630 | None:intergenic | 40.0% | |
| CTGCTGTTGAAGAGATTAAG+TGG | - | chr2.3:710072-710091 | MS.gene86949:CDS | 40.0% | |
| GAAGAGGAACACACAATTTG+TGG | - | chr2.3:709854-709873 | MS.gene86949:CDS | 40.0% | |
| ! | AAAGCAGGAGCTACTGTTTT+TGG | + | chr2.3:709930-709949 | None:intergenic | 40.0% |
| ! | CATCCATCTTTTGCTCCTTT+TGG | + | chr2.3:709998-710017 | None:intergenic | 40.0% |
| ! | GCTCATTTTGTGCAAAGACA+AGG | + | chr2.3:709560-709579 | None:intergenic | 40.0% |
| !! | GGAAGAGTGCTATAATGAAG+AGG | - | chr2.3:709838-709857 | MS.gene86949:CDS | 40.0% |
| AATCCACCATTAGGCCTAGT+TGG | + | chr2.3:709807-709826 | None:intergenic | 45.0% | |
| CTTCTCCCAAATCCACCATT+AGG | + | chr2.3:709816-709835 | None:intergenic | 45.0% | |
| GAGGAACACACAATTTGTGG+TGG | - | chr2.3:709857-709876 | MS.gene86949:CDS | 45.0% | |
| GCTTTGTCTGGTGATCTTGA+TGG | - | chr2.3:709945-709964 | MS.gene86949:CDS | 45.0% | |
| TACAAGAGATGGCTCAAGGT+TGG | + | chr2.3:709526-709545 | None:intergenic | 45.0% | |
| TCAAGATCACCAGACAAAGC+AGG | + | chr2.3:709945-709964 | None:intergenic | 45.0% | |
| ! | AAGATGGATGGAGATGATGG+TGG | - | chr2.3:710008-710027 | MS.gene86949:CDS | 45.0% |
| ! | CACTTATGGTGATGCCAACT+AGG | - | chr2.3:709790-709809 | MS.gene86949:CDS | 45.0% |
| !! | ACTAGGCCTAATGGTGGATT+TGG | - | chr2.3:709807-709826 | MS.gene86949:CDS | 45.0% |
| !! | ATGGTGGATTTGGGAGAAGA+TGG | - | chr2.3:709817-709836 | MS.gene86949:CDS | 45.0% |
| !! | CTAGGCCTAATGGTGGATTT+GGG | - | chr2.3:709808-709827 | MS.gene86949:CDS | 45.0% |
| !!! | AGGAGCTACTGTTTTTGGCA+TGG | + | chr2.3:709925-709944 | None:intergenic | 45.0% |
| ACAGTAGCTCCTGCTTTGTC+TGG | - | chr2.3:709933-709952 | MS.gene86949:CDS | 50.0% | |
| ATGCCAACTAGGCCTAATGG+TGG | - | chr2.3:709801-709820 | MS.gene86949:CDS | 50.0% | |
| GTGATGCCAACTAGGCCTAA+TGG | - | chr2.3:709798-709817 | MS.gene86949:CDS | 50.0% | |
| !! | GATGGAGATGATGGTGGTGA+TGG | - | chr2.3:710014-710033 | MS.gene86949:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 709495 | 710127 | 709495 | ID=MS.gene86949 |
| chr2.3 | mRNA | 709495 | 710127 | 709495 | ID=MS.gene86949.t1;Parent=MS.gene86949 |
| chr2.3 | exon | 709495 | 710127 | 709495 | ID=MS.gene86949.t1.exon1;Parent=MS.gene86949.t1 |
| chr2.3 | CDS | 709495 | 710127 | 709495 | ID=cds.MS.gene86949.t1;Parent=MS.gene86949.t1 |
| Gene Sequence |
| Protein sequence |
