Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87382.t1 | XP_003629527.2 | 95.5 | 89 | 2 | 2 | 6 | 93 | 723 | 810 | 5.00E-35 | 156.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87382.t1 | G7LFL6 | 95.5 | 89 | 2 | 2 | 6 | 93 | 723 | 810 | 3.6e-35 | 156.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051568 | MS.gene87382 | 0.803569 | 3.27E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87382.t1 | MTR_8g078480 | 95.745 | 94 | 2 | 2 | 1 | 93 | 718 | 810 | 2.95e-53 | 177 |
| MS.gene87382.t1 | MTR_5g018860 | 54.255 | 94 | 28 | 4 | 7 | 92 | 641 | 727 | 7.11e-20 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCCTTGTCTATTTATTAA+TGG | 0.229436 | 8.3:+25753643 | None:intergenic |
| AGCTTTGTTTCAGCGGAATA+TGG | 0.239058 | 8.3:-25753499 | MS.gene87382:CDS |
| GGTTCTACACTCATCAGATT+TGG | 0.285290 | 8.3:-25753451 | MS.gene87382:CDS |
| ATTGGCATTGATAGTATTGT+TGG | 0.352048 | 8.3:+25753687 | None:intergenic |
| GTTCTACACTCATCAGATTT+GGG | 0.374497 | 8.3:-25753450 | MS.gene87382:CDS |
| AATAAATAGACAAGGCTTCT+CGG | 0.428846 | 8.3:-25753638 | MS.gene87382:CDS |
| GCTTTGTTTCAGCGGAATAT+GGG | 0.430862 | 8.3:-25753498 | MS.gene87382:CDS |
| TTTATTAATGGTGTAAGTGA+TGG | 0.465053 | 8.3:+25753655 | None:intergenic |
| AGATTTGGGACCAATACTTC+TGG | 0.476436 | 8.3:-25753436 | None:intergenic |
| ACCACCTCTCTCCGATTCAA+TGG | 0.487399 | 8.3:-25753548 | MS.gene87382:CDS |
| GTTGTTGCAACAACATTGCT+TGG | 0.498392 | 8.3:+25753611 | None:intergenic |
| ACTGGTGCCTCTGCTGACAT+TGG | 0.514976 | 8.3:-25753472 | MS.gene87382:CDS |
| GTCATCAATAGCCATTGAAT+CGG | 0.515256 | 8.3:+25753537 | None:intergenic |
| TTATTAATGGTGTAAGTGAT+GGG | 0.528441 | 8.3:+25753656 | None:intergenic |
| TGACACGTCACCAGAAGTAT+TGG | 0.529079 | 8.3:+25753426 | None:intergenic |
| ACATGGCAGCTTTGTTTCAG+CGG | 0.554817 | 8.3:-25753506 | MS.gene87382:CDS |
| TCAGCGGAATATGGGACCAC+TGG | 0.559048 | 8.3:-25753490 | MS.gene87382:CDS |
| ATTGATGACACTTGTCGACA+TGG | 0.588890 | 8.3:-25753523 | MS.gene87382:CDS |
| CGTTAAAACCACCGTCTCTG+AGG | 0.616452 | 8.3:-25753575 | MS.gene87382:CDS |
| AGGTGGTGCCACCTCAGAGA+CGG | 0.617050 | 8.3:+25753564 | None:intergenic |
| GCCATTGAATCGGAGAGAGG+TGG | 0.635739 | 8.3:+25753547 | None:intergenic |
| AATGTCAGCAGAGGCACCAG+TGG | 0.645556 | 8.3:+25753474 | None:intergenic |
| TAAAACCACCGTCTCTGAGG+TGG | 0.650762 | 8.3:-25753572 | MS.gene87382:CDS |
| ATAGCCATTGAATCGGAGAG+AGG | 0.662803 | 8.3:+25753544 | None:intergenic |
| TGGTGCCACCTCAGAGACGG+TGG | 0.664303 | 8.3:+25753567 | None:intergenic |
| TGTAGAACCAATGTCAGCAG+AGG | 0.675876 | 8.3:+25753465 | None:intergenic |
| ACACCATTAATAAATAGACA+AGG | 0.702493 | 8.3:-25753646 | MS.gene87382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACACCATTAATAAATAGACA+AGG | - | chr8.3:25753485-25753504 | MS.gene87382:CDS | 25.0% |
| ! | TTATTAATGGTGTAAGTGAT+GGG | + | chr8.3:25753478-25753497 | None:intergenic | 25.0% |
| ! | TTTATTAATGGTGTAAGTGA+TGG | + | chr8.3:25753479-25753498 | None:intergenic | 25.0% |
| !! | AAGCCTTGTCTATTTATTAA+TGG | + | chr8.3:25753491-25753510 | None:intergenic | 25.0% |
| AATAAATAGACAAGGCTTCT+CGG | - | chr8.3:25753493-25753512 | MS.gene87382:CDS | 30.0% | |
| !! | ATTGGCATTGATAGTATTGT+TGG | + | chr8.3:25753447-25753466 | None:intergenic | 30.0% |
| GTCATCAATAGCCATTGAAT+CGG | + | chr8.3:25753597-25753616 | None:intergenic | 35.0% | |
| GTTCTACACTCATCAGATTT+GGG | - | chr8.3:25753681-25753700 | MS.gene87382:CDS | 35.0% | |
| GGTTCTACACTCATCAGATT+TGG | - | chr8.3:25753680-25753699 | MS.gene87382:CDS | 40.0% | |
| GTTGTTGCAACAACATTGCT+TGG | + | chr8.3:25753523-25753542 | None:intergenic | 40.0% | |
| ! | ATTGATGACACTTGTCGACA+TGG | - | chr8.3:25753608-25753627 | MS.gene87382:CDS | 40.0% |
| !! | AGCTTTGTTTCAGCGGAATA+TGG | - | chr8.3:25753632-25753651 | MS.gene87382:CDS | 40.0% |
| !! | GCTTTGTTTCAGCGGAATAT+GGG | - | chr8.3:25753633-25753652 | MS.gene87382:CDS | 40.0% |
| !!! | AAGTGATGGGTCGTTTTCAT+TGG | + | chr8.3:25753465-25753484 | None:intergenic | 40.0% |
| ATAGCCATTGAATCGGAGAG+AGG | + | chr8.3:25753590-25753609 | None:intergenic | 45.0% | |
| TGTAGAACCAATGTCAGCAG+AGG | + | chr8.3:25753669-25753688 | None:intergenic | 45.0% | |
| !! | ACATGGCAGCTTTGTTTCAG+CGG | - | chr8.3:25753625-25753644 | MS.gene87382:CDS | 45.0% |
| !!! | CTCAGAGACGGTGGTTTTAA+CGG | + | chr8.3:25753558-25753577 | None:intergenic | 45.0% |
| !!! | GTGGTTTTAACGGTGGAAGA+TGG | + | chr8.3:25753548-25753567 | None:intergenic | 45.0% |
| ACCACCTCTCTCCGATTCAA+TGG | - | chr8.3:25753583-25753602 | MS.gene87382:CDS | 50.0% | |
| CGTTAAAACCACCGTCTCTG+AGG | - | chr8.3:25753556-25753575 | MS.gene87382:CDS | 50.0% | |
| ! | TAAAACCACCGTCTCTGAGG+TGG | - | chr8.3:25753559-25753578 | MS.gene87382:CDS | 50.0% |
| !!! | AGAGACGGTGGTTTTAACGG+TGG | + | chr8.3:25753555-25753574 | None:intergenic | 50.0% |
| ACTGGTGCCTCTGCTGACAT+TGG | - | chr8.3:25753659-25753678 | MS.gene87382:CDS | 55.0% | |
| GCCATTGAATCGGAGAGAGG+TGG | + | chr8.3:25753587-25753606 | None:intergenic | 55.0% | |
| TCAGCGGAATATGGGACCAC+TGG | - | chr8.3:25753641-25753660 | MS.gene87382:CDS | 55.0% | |
| !! | AATGTCAGCAGAGGCACCAG+TGG | + | chr8.3:25753660-25753679 | None:intergenic | 55.0% |
| !! | AGGTGGTGCCACCTCAGAGA+CGG | + | chr8.3:25753570-25753589 | None:intergenic | 60.0% |
| !! | TGGTGCCACCTCAGAGACGG+TGG | + | chr8.3:25753567-25753586 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 25753437 | 25753716 | 25753437 | ID=MS.gene87382 |
| chr8.3 | mRNA | 25753437 | 25753716 | 25753437 | ID=MS.gene87382.t1;Parent=MS.gene87382 |
| chr8.3 | exon | 25753437 | 25753716 | 25753437 | ID=MS.gene87382.t1.exon1;Parent=MS.gene87382.t1 |
| chr8.3 | CDS | 25753437 | 25753716 | 25753437 | ID=cds.MS.gene87382.t1;Parent=MS.gene87382.t1 |
| Gene Sequence |
| Protein sequence |
