Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87589.t1 | TKY55040.1 | 69.7 | 208 | 54 | 2 | 18 | 225 | 136 | 334 | 7.10E-67 | 263.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87589.t1 | P42730 | 68.8 | 208 | 56 | 3 | 18 | 225 | 136 | 334 | 1.4e-68 | 260.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87589.t1 | A0A4P1RX68 | 69.2 | 208 | 55 | 2 | 18 | 225 | 136 | 334 | 1.5e-66 | 262.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053266 | MS.gene87589 | 0.89443 | 2.49E-75 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87589.t1 | MTR_4g109450 | 68.750 | 208 | 56 | 3 | 18 | 225 | 136 | 334 | 3.49e-83 | 264 |
| MS.gene87589.t1 | MTR_4g109450 | 68.750 | 208 | 56 | 3 | 18 | 225 | 136 | 334 | 6.84e-83 | 265 |
| MS.gene87589.t1 | MTR_7g028970 | 62.981 | 208 | 68 | 3 | 18 | 225 | 136 | 334 | 1.03e-75 | 245 |
| MS.gene87589.t1 | MTR_3g106160 | 56.757 | 185 | 69 | 4 | 43 | 225 | 248 | 423 | 5.07e-57 | 194 |
| MS.gene87589.t1 | MTR_7g012820 | 55.676 | 185 | 71 | 4 | 43 | 225 | 169 | 344 | 1.13e-56 | 193 |
| MS.gene87589.t1 | MTR_7g012820 | 55.676 | 185 | 71 | 4 | 43 | 225 | 240 | 415 | 1.40e-56 | 193 |
| MS.gene87589.t1 | MTR_6g013660 | 55.135 | 185 | 72 | 4 | 43 | 225 | 236 | 411 | 3.63e-56 | 192 |
| MS.gene87589.t1 | MTR_3g102830 | 46.907 | 194 | 91 | 5 | 34 | 225 | 248 | 431 | 1.55e-47 | 167 |
| MS.gene87589.t1 | MTR_8g100040 | 46.907 | 194 | 91 | 5 | 34 | 225 | 247 | 430 | 2.06e-47 | 167 |
| MS.gene87589.t1 | MTR_3g498725 | 46.907 | 194 | 91 | 5 | 34 | 225 | 243 | 426 | 5.05e-47 | 166 |
| MS.gene87589.t1 | MTR_6g048860 | 36.559 | 186 | 102 | 6 | 40 | 219 | 130 | 305 | 1.23e-31 | 119 |
| MS.gene87589.t1 | MTR_3g098310 | 39.572 | 187 | 96 | 5 | 46 | 225 | 298 | 474 | 7.09e-30 | 117 |
| MS.gene87589.t1 | MTR_3g098310 | 39.572 | 187 | 96 | 5 | 46 | 225 | 298 | 474 | 8.18e-30 | 117 |
| MS.gene87589.t1 | MTR_3g461000 | 34.066 | 182 | 107 | 5 | 46 | 225 | 172 | 342 | 4.93e-23 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87589.t1 | AT1G74310 | 68.750 | 208 | 56 | 3 | 18 | 225 | 136 | 334 | 3.85e-83 | 264 |
| MS.gene87589.t1 | AT1G74310 | 68.750 | 208 | 56 | 3 | 18 | 225 | 136 | 334 | 1.69e-82 | 263 |
| MS.gene87589.t1 | AT4G14670 | 52.404 | 208 | 79 | 3 | 18 | 225 | 112 | 299 | 2.95e-58 | 194 |
| MS.gene87589.t1 | AT2G25140 | 55.319 | 188 | 73 | 4 | 40 | 225 | 236 | 414 | 2.18e-57 | 196 |
| MS.gene87589.t1 | AT5G15450 | 55.135 | 185 | 72 | 4 | 43 | 225 | 234 | 409 | 4.97e-56 | 192 |
| MS.gene87589.t1 | AT3G48870 | 49.727 | 183 | 80 | 5 | 45 | 225 | 277 | 449 | 1.50e-47 | 167 |
| MS.gene87589.t1 | AT3G48870 | 49.727 | 183 | 80 | 5 | 45 | 225 | 277 | 449 | 1.50e-47 | 167 |
| MS.gene87589.t1 | AT3G48870 | 49.727 | 183 | 80 | 5 | 45 | 225 | 277 | 449 | 1.50e-47 | 167 |
| MS.gene87589.t1 | AT3G48870 | 49.727 | 183 | 80 | 5 | 45 | 225 | 246 | 418 | 1.78e-47 | 167 |
| MS.gene87589.t1 | AT5G50920 | 49.180 | 183 | 81 | 5 | 45 | 225 | 256 | 428 | 2.60e-47 | 167 |
| MS.gene87589.t1 | AT5G51070 | 42.021 | 188 | 92 | 6 | 45 | 225 | 270 | 447 | 1.40e-33 | 127 |
| MS.gene87589.t1 | AT3G45450 | 37.427 | 171 | 80 | 4 | 56 | 225 | 149 | 293 | 3.14e-23 | 95.9 |
Find 36 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAAATTCATCTTGTTCT+TGG | 0.215941 | 6.4:-27206242 | MS.gene87589:CDS |
| GGGAAGCTTGACCCTGTTAT+TGG | 0.254949 | 6.4:-27206575 | MS.gene87589:CDS |
| TCTTGATCAATTCCAATAAC+AGG | 0.289014 | 6.4:+27206563 | None:intergenic |
| AAGAAAGTTGAAAGTGCTTT+TGG | 0.305891 | 6.4:-27206653 | MS.gene87589:CDS |
| GCTATGGGCACATTAGTTGC+TGG | 0.382128 | 6.4:-27206353 | MS.gene87589:CDS |
| CCTGGCTCTCCTACATGAAT+AGG | 0.384659 | 6.4:+27206494 | None:intergenic |
| GAAAATAGTAAAACAGATCT+TGG | 0.400886 | 6.4:-27206407 | MS.gene87589:CDS |
| ATTCATCTTGTTCTTGGTGC+TGG | 0.441304 | 6.4:-27206236 | MS.gene87589:CDS |
| CAACAACAATTGAAGATAAC+GGG | 0.447674 | 6.4:-27206133 | MS.gene87589:CDS |
| CATGTAGGAGAGCCAGGTAT+TGG | 0.451646 | 6.4:-27206488 | MS.gene87589:CDS |
| CCTATTCATGTAGGAGAGCC+AGG | 0.455958 | 6.4:-27206494 | MS.gene87589:CDS |
| TATCCACCACGATCAACAAT+TGG | 0.460586 | 6.4:+27206173 | None:intergenic |
| AAGTGGAAGAAGCTGAAGGA+AGG | 0.467536 | 6.4:-27206280 | MS.gene87589:CDS |
| ATCTGAGAGGAGGTTTGTAT+AGG | 0.468885 | 6.4:-27206087 | None:intergenic |
| AACTGTTGTTGTTGAAGGGT+TGG | 0.480025 | 6.4:-27206462 | MS.gene87589:CDS |
| AGCTATTTAGTTGAACGAGC+AGG | 0.483982 | 6.4:-27206596 | MS.gene87589:CDS |
| CTTGGTGCTGGTAGAATTGA+AGG | 0.487432 | 6.4:-27206224 | MS.gene87589:CDS |
| ACAACAACAATTGAAGATAA+CGG | 0.490824 | 6.4:-27206134 | MS.gene87589:CDS |
| GAATTGATCAAGAGATAGAA+AGG | 0.504920 | 6.4:-27206553 | MS.gene87589:CDS |
| TTGTTGAAGGGTTGGCTCAA+AGG | 0.514640 | 6.4:-27206454 | MS.gene87589:CDS |
| ATCGTGTTTGCAGCAAGTAC+AGG | 0.522665 | 6.4:-27206882 | MS.gene87589:intron |
| GGTGGATATCTTAGATGCAT+TGG | 0.531632 | 6.4:-27206158 | MS.gene87589:CDS |
| AGTGGAAGAAGCTGAAGGAA+GGG | 0.534741 | 6.4:-27206279 | MS.gene87589:CDS |
| CGATGACGATGCGCGTATGA+TGG | 0.537189 | 6.4:+27206901 | None:intergenic |
| GCTATTTAGTTGAACGAGCA+GGG | 0.548118 | 6.4:-27206595 | MS.gene87589:CDS |
| GAAGGGTTGGCTCAAAGGAT+AGG | 0.553623 | 6.4:-27206449 | MS.gene87589:CDS |
| CTCAAACCAATTGTTGATCG+TGG | 0.557751 | 6.4:-27206179 | MS.gene87589:CDS |
| AAAAGCTATATTGTAAAGAA+CGG | 0.562599 | 6.4:-27206676 | MS.gene87589:CDS |
| AAGAACAATCCTATTCATGT+AGG | 0.565156 | 6.4:-27206503 | MS.gene87589:CDS |
| TTGGAAATCTTGCTAATGCA+AGG | 0.571208 | 6.4:-27206388 | MS.gene87589:CDS |
| AAAGAAGTGGAAGAAGCTGA+AGG | 0.582184 | 6.4:-27206284 | MS.gene87589:CDS |
| GTTGCTGGTGCTAAGAGCAT+CGG | 0.600532 | 6.4:-27206338 | MS.gene87589:CDS |
| AAACCAATTGTTGATCGTGG+TGG | 0.614973 | 6.4:-27206176 | MS.gene87589:CDS |
| TTGGCTCAAAGGATAGGTAG+AGG | 0.617855 | 6.4:-27206443 | MS.gene87589:CDS |
| CTTGATCAATTCCAATAACA+GGG | 0.625696 | 6.4:+27206564 | None:intergenic |
| AATATGCAGCATCTGAGAGG+AGG | 0.644001 | 6.4:-27206097 | MS.gene87589:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCAAGTTTTGAAAAAATA+TGG | - | chr6.4:27206381-27206400 | MS.gene87589:CDS | 15.0% |
| !! | AAAAGCTATATTGTAAAGAA+CGG | - | chr6.4:27206325-27206344 | MS.gene87589:CDS | 20.0% |
| ! | AATGAAATTCATCTTGTTCT+TGG | - | chr6.4:27206759-27206778 | MS.gene87589:intron | 25.0% |
| ! | ACAACAACAATTGAAGATAA+CGG | - | chr6.4:27206867-27206886 | MS.gene87589:intron | 25.0% |
| ! | GAAAATAGTAAAACAGATCT+TGG | - | chr6.4:27206594-27206613 | MS.gene87589:CDS | 25.0% |
| !!! | AAGGATATTTGTTTTAGCTA+TGG | - | chr6.4:27206632-27206651 | MS.gene87589:CDS | 25.0% |
| !!! | AGGATATTTGTTTTAGCTAT+GGG | - | chr6.4:27206633-27206652 | MS.gene87589:CDS | 25.0% |
| AAGAACAATCCTATTCATGT+AGG | - | chr6.4:27206498-27206517 | MS.gene87589:CDS | 30.0% | |
| CAACAACAATTGAAGATAAC+GGG | - | chr6.4:27206868-27206887 | MS.gene87589:intron | 30.0% | |
| CTTGATCAATTCCAATAACA+GGG | + | chr6.4:27206440-27206459 | None:intergenic | 30.0% | |
| GAATTGATCAAGAGATAGAA+AGG | - | chr6.4:27206448-27206467 | MS.gene87589:CDS | 30.0% | |
| TCTTGATCAATTCCAATAAC+AGG | + | chr6.4:27206441-27206460 | None:intergenic | 30.0% | |
| ! | GAAAAACTGTTGTTGTTGAA+GGG | - | chr6.4:27206535-27206554 | MS.gene87589:CDS | 30.0% |
| ! | TTGTCGTGATTTTGTCAAAA+AGG | - | chr6.4:27206472-27206491 | MS.gene87589:CDS | 30.0% |
| !! | AGATTTTGAAGAAAGGCTTA+AGG | - | chr6.4:27206686-27206705 | MS.gene87589:CDS | 30.0% |
| !!! | AAGAAAGTTGAAAGTGCTTT+TGG | - | chr6.4:27206348-27206367 | MS.gene87589:CDS | 30.0% |
| !!! | TAAGGAAGTTTTGAAAGAAG+TGG | - | chr6.4:27206704-27206723 | MS.gene87589:intron | 30.0% |
| !!! | TTTTTGTGTCACTGTTTCTA+GGG | - | chr6.4:27206287-27206306 | MS.gene87589:CDS | 30.0% |
| !!! | TTTTTTGTGTCACTGTTTCT+AGG | - | chr6.4:27206286-27206305 | MS.gene87589:CDS | 30.0% |
| AAAAATATGCAGCATCTGAG+AGG | - | chr6.4:27206901-27206920 | MS.gene87589:CDS | 35.0% | |
| TCATGTTCTCACTTCAATGA+TGG | - | chr6.4:27206237-27206256 | MS.gene87589:CDS | 35.0% | |
| TTGGAAATCTTGCTAATGCA+AGG | - | chr6.4:27206613-27206632 | MS.gene87589:CDS | 35.0% | |
| ! | ACAACAGTTTTTCCAATACC+TGG | + | chr6.4:27206528-27206547 | None:intergenic | 35.0% |
| ! | GGAAAAACTGTTGTTGTTGA+AGG | - | chr6.4:27206534-27206553 | MS.gene87589:CDS | 35.0% |
| ! | TCACCGATTGCAGTAAATAT+CGG | + | chr6.4:27206192-27206211 | None:intergenic | 35.0% |
| AAACCAATTGTTGATCGTGG+TGG | - | chr6.4:27206825-27206844 | MS.gene87589:intron | 40.0% | |
| AAAGAAGTGGAAGAAGCTGA+AGG | - | chr6.4:27206717-27206736 | MS.gene87589:intron | 40.0% | |
| AGCTATTTAGTTGAACGAGC+AGG | - | chr6.4:27206405-27206424 | MS.gene87589:CDS | 40.0% | |
| CTCAAACCAATTGTTGATCG+TGG | - | chr6.4:27206822-27206841 | MS.gene87589:intron | 40.0% | |
| GCTATTTAGTTGAACGAGCA+GGG | - | chr6.4:27206406-27206425 | MS.gene87589:CDS | 40.0% | |
| GCTCCGATATTTACTGCAAT+CGG | - | chr6.4:27206186-27206205 | MS.gene87589:CDS | 40.0% | |
| GGTGGATATCTTAGATGCAT+TGG | - | chr6.4:27206843-27206862 | MS.gene87589:intron | 40.0% | |
| TATCCACCACGATCAACAAT+TGG | + | chr6.4:27206831-27206850 | None:intergenic | 40.0% | |
| ! | AACTGTTGTTGTTGAAGGGT+TGG | - | chr6.4:27206539-27206558 | MS.gene87589:CDS | 40.0% |
| ! | ATTCATCTTGTTCTTGGTGC+TGG | - | chr6.4:27206765-27206784 | MS.gene87589:intron | 40.0% |
| !! | GCATCGGAGATTTTGAAGAA+AGG | - | chr6.4:27206679-27206698 | MS.gene87589:CDS | 40.0% |
| AAGTGGAAGAAGCTGAAGGA+AGG | - | chr6.4:27206721-27206740 | MS.gene87589:intron | 45.0% | |
| AATATGCAGCATCTGAGAGG+AGG | - | chr6.4:27206904-27206923 | MS.gene87589:CDS | 45.0% | |
| AGTGGAAGAAGCTGAAGGAA+GGG | - | chr6.4:27206722-27206741 | MS.gene87589:intron | 45.0% | |
| ATCGTGTTTGCAGCAAGTAC+AGG | - | chr6.4:27206119-27206138 | MS.gene87589:CDS | 45.0% | |
| TTGGCTCAAAGGATAGGTAG+AGG | - | chr6.4:27206558-27206577 | MS.gene87589:CDS | 45.0% | |
| TTGTCTCGCAACACTAGAAG+AGG | + | chr6.4:27206158-27206177 | None:intergenic | 45.0% | |
| !! | CTTGGTGCTGGTAGAATTGA+AGG | - | chr6.4:27206777-27206796 | MS.gene87589:intron | 45.0% |
| !! | TTGTTGAAGGGTTGGCTCAA+AGG | - | chr6.4:27206547-27206566 | MS.gene87589:CDS | 45.0% |
| CATGTAGGAGAGCCAGGTAT+TGG | - | chr6.4:27206513-27206532 | MS.gene87589:CDS | 50.0% | |
| CCTATTCATGTAGGAGAGCC+AGG | - | chr6.4:27206507-27206526 | MS.gene87589:CDS | 50.0% | |
| GCTATGGGCACATTAGTTGC+TGG | - | chr6.4:27206648-27206667 | MS.gene87589:CDS | 50.0% | |
| GGGAAGCTTGACCCTGTTAT+TGG | - | chr6.4:27206426-27206445 | MS.gene87589:CDS | 50.0% | |
| ! | CCTGGCTCTCCTACATGAAT+AGG | + | chr6.4:27206510-27206529 | None:intergenic | 50.0% |
| ! | GTTGCTGGTGCTAAGAGCAT+CGG | - | chr6.4:27206663-27206682 | MS.gene87589:CDS | 50.0% |
| !! | GAAGGGTTGGCTCAAAGGAT+AGG | - | chr6.4:27206552-27206571 | MS.gene87589:CDS | 50.0% |
| CGATGACGATGCGCGTATGA+TGG | + | chr6.4:27206103-27206122 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 27206088 | 27206935 | 27206088 | ID=MS.gene87589 |
| chr6.4 | mRNA | 27206088 | 27206935 | 27206088 | ID=MS.gene87589.t1;Parent=MS.gene87589 |
| chr6.4 | exon | 27206883 | 27206935 | 27206883 | ID=MS.gene87589.t1.exon1;Parent=MS.gene87589.t1 |
| chr6.4 | CDS | 27206883 | 27206935 | 27206883 | ID=cds.MS.gene87589.t1;Parent=MS.gene87589.t1 |
| chr6.4 | exon | 27206088 | 27206715 | 27206088 | ID=MS.gene87589.t1.exon2;Parent=MS.gene87589.t1 |
| chr6.4 | CDS | 27206088 | 27206715 | 27206088 | ID=cds.MS.gene87589.t1;Parent=MS.gene87589.t1 |
| Gene Sequence |
| Protein sequence |
