Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87889.t1 | RHN79655.1 | 88.3 | 412 | 46 | 2 | 1 | 411 | 1 | 411 | 1.10E-187 | 666 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87889.t1 | Q9LI74 | 28.1 | 249 | 179 | 0 | 100 | 348 | 130 | 378 | 2.9e-06 | 54.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87889.t1 | A0A396JTV1 | 88.3 | 412 | 46 | 2 | 1 | 411 | 1 | 411 | 8.1e-188 | 666.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058979 | MS.gene87889 | 0.815341 | 9.72E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87889.t1 | MTR_1g061840 | 91.505 | 412 | 33 | 2 | 1 | 411 | 1 | 411 | 0.0 | 736 |
| MS.gene87889.t1 | MTR_8g014900 | 23.398 | 359 | 234 | 8 | 12 | 336 | 22 | 373 | 4.42e-19 | 89.7 |
| MS.gene87889.t1 | MTR_2g100630 | 22.016 | 377 | 222 | 8 | 10 | 336 | 9 | 363 | 2.53e-15 | 78.2 |
| MS.gene87889.t1 | MTR_5g053270 | 25.581 | 258 | 178 | 2 | 98 | 348 | 125 | 375 | 8.26e-14 | 73.9 |
| MS.gene87889.t1 | MTR_2g100630 | 22.449 | 245 | 166 | 2 | 98 | 336 | 96 | 322 | 8.68e-13 | 70.1 |
| MS.gene87889.t1 | MTR_1g093850 | 24.324 | 259 | 170 | 3 | 96 | 335 | 130 | 381 | 5.58e-12 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87889.t1 | AT2G36650 | 29.231 | 325 | 184 | 3 | 11 | 335 | 13 | 291 | 5.44e-33 | 127 |
| MS.gene87889.t1 | AT3G25690 | 24.498 | 249 | 188 | 0 | 100 | 348 | 132 | 380 | 4.20e-15 | 78.2 |
| MS.gene87889.t1 | AT3G25690 | 24.498 | 249 | 188 | 0 | 100 | 348 | 130 | 378 | 5.11e-15 | 77.8 |
| MS.gene87889.t1 | AT3G25690 | 24.498 | 249 | 188 | 0 | 100 | 348 | 130 | 378 | 5.11e-15 | 77.8 |
| MS.gene87889.t1 | AT3G25690 | 24.498 | 249 | 188 | 0 | 100 | 348 | 130 | 378 | 5.11e-15 | 77.8 |
| MS.gene87889.t1 | AT3G25690 | 24.498 | 249 | 188 | 0 | 100 | 348 | 130 | 378 | 5.11e-15 | 77.8 |
| MS.gene87889.t1 | AT1G52080 | 26.259 | 278 | 184 | 4 | 98 | 368 | 138 | 401 | 8.49e-12 | 67.4 |
| MS.gene87889.t1 | AT1G52080 | 26.259 | 278 | 184 | 4 | 98 | 368 | 138 | 401 | 8.49e-12 | 67.4 |
Find 43 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGATTAATGTTTGTTTA+AGG | 0.192588 | 1.2:+47203704 | MS.gene87889:CDS |
| TGCATTGGTTCTTCATTTCA+TGG | 0.234872 | 1.2:+47203784 | MS.gene87889:CDS |
| GGCTCAATCTCATTTGATTA+AGG | 0.274337 | 1.2:+47203345 | MS.gene87889:CDS |
| TCCTTCTTCTAGTTCCAAAA+AGG | 0.288710 | 1.2:+47203810 | MS.gene87889:CDS |
| ACACCAGCTTTGAGGATAAT+AGG | 0.296488 | 1.2:-47202776 | None:intergenic |
| AGTAATGAAATTAAGTGCTT+TGG | 0.311730 | 1.2:+47203907 | MS.gene87889:CDS |
| CAACAAGGTTCTGTATGAAT+TGG | 0.333146 | 1.2:+47203630 | MS.gene87889:CDS |
| TTTCAATAATCTTAAGGTAT+TGG | 0.335768 | 1.2:-47203252 | None:intergenic |
| ATGAAATTAGAGAGATGAAA+AGG | 0.350164 | 1.2:+47203461 | MS.gene87889:CDS |
| ACATCATGAGAATTGACTTC+AGG | 0.356940 | 1.2:-47202899 | None:intergenic |
| TCTTTCTGAGAGATTTGGTT+TGG | 0.365727 | 1.2:+47203009 | MS.gene87889:CDS |
| AGTGTTCTTTCTGAGAGATT+TGG | 0.368051 | 1.2:+47203004 | MS.gene87889:CDS |
| TAGAGAGATGAAAAGGGTCT+TGG | 0.388058 | 1.2:+47203468 | MS.gene87889:CDS |
| GTAATGAAATTAAGTGCTTT+GGG | 0.393139 | 1.2:+47203908 | MS.gene87889:CDS |
| GAGCAAAACCTTGGAGTAGC+TGG | 0.400491 | 1.2:-47203943 | None:intergenic |
| TTTGTCAAATTGTAAAGTTA+AGG | 0.415691 | 1.2:-47203089 | None:intergenic |
| ATCTGCAAATAATATTTCAA+TGG | 0.417278 | 1.2:-47203577 | None:intergenic |
| AGTATTGGAAATCAGAGAAT+AGG | 0.426097 | 1.2:+47203281 | MS.gene87889:CDS |
| TGAAATTAGAGAGATGAAAA+GGG | 0.439475 | 1.2:+47203462 | MS.gene87889:CDS |
| TGCTTAAGAAGCAAGATTGA+AGG | 0.458252 | 1.2:+47203046 | MS.gene87889:CDS |
| CTATAGGAACACCAGCTTTG+AGG | 0.462456 | 1.2:-47202784 | None:intergenic |
| TACTATGGAAGATGAAGAAA+AGG | 0.467395 | 1.2:+47202955 | MS.gene87889:CDS |
| TTGCTGGTTTGATCTATGCA+TGG | 0.476381 | 1.2:+47202816 | MS.gene87889:CDS |
| TGAATCAAGAGATGTTATGA+AGG | 0.483238 | 1.2:+47203600 | MS.gene87889:CDS |
| CCAGCAAAAGAAACAGCTAT+AGG | 0.492610 | 1.2:-47202800 | None:intergenic |
| CATCAAAATGTGTCTTGCAT+TGG | 0.492837 | 1.2:+47203769 | MS.gene87889:CDS |
| AAGAACACTACTATCTATAG+AGG | 0.498832 | 1.2:-47202990 | None:intergenic |
| TTGGGTGGATGGCAGTGAAA+AGG | 0.511387 | 1.2:+47203858 | MS.gene87889:CDS |
| ATGAGAGTCAGTTTCTGAAA+AGG | 0.515828 | 1.2:-47202873 | None:intergenic |
| TGAATTGGAGCAAGTTAAGA+AGG | 0.521848 | 1.2:+47203645 | MS.gene87889:CDS |
| TTGAAGAACTTATTTACTTG+AGG | 0.545043 | 1.2:+47203683 | MS.gene87889:CDS |
| CATCATGAGAATTGACTTCA+GGG | 0.556455 | 1.2:-47202898 | None:intergenic |
| AAGCCTATTATCCTCAAAGC+TGG | 0.568812 | 1.2:+47202773 | MS.gene87889:CDS |
| CTTGAGACATCAGAAGAACA+TGG | 0.572471 | 1.2:+47203529 | MS.gene87889:CDS |
| TCATAGAAACAGAAACTATG+AGG | 0.580497 | 1.2:+47203212 | MS.gene87889:CDS |
| GAAGCTACTTAAGAAATCAA+AGG | 0.584610 | 1.2:+47203324 | MS.gene87889:CDS |
| ATTCTGCTCCAGCTACTCCA+AGG | 0.603120 | 1.2:+47203935 | MS.gene87889:CDS |
| CAAGCTTTGATGATCAAAGA+GGG | 0.612670 | 1.2:+47203370 | MS.gene87889:CDS |
| AAGGTTAAAGTAAAACCAGA+AGG | 0.614970 | 1.2:+47203877 | MS.gene87889:CDS |
| TAAGCACTAGAGCAAAACCT+TGG | 0.616488 | 1.2:-47203952 | None:intergenic |
| GCAAGCTTTGATGATCAAAG+AGG | 0.618483 | 1.2:+47203369 | MS.gene87889:CDS |
| ATCATGAGAATTGACTTCAG+GGG | 0.687779 | 1.2:-47202897 | None:intergenic |
| TATGAAGGAAGATTACAACA+AGG | 0.743853 | 1.2:+47203615 | MS.gene87889:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAAAAACATGTTATCAT+TGG | + | chr1.2:47203150-47203169 | MS.gene87889:CDS | 20.0% |
| !! | ATCTGCAAATAATATTTCAA+TGG | - | chr1.2:47203580-47203599 | None:intergenic | 20.0% |
| !! | GATTAAAAAGCTGAAAAATT+GGG | + | chr1.2:47203840-47203859 | MS.gene87889:CDS | 20.0% |
| !! | TGATTAAAAAGCTGAAAAAT+TGG | + | chr1.2:47203839-47203858 | MS.gene87889:CDS | 20.0% |
| !! | TTATTGAAAAACTTGAGTAT+TGG | + | chr1.2:47203266-47203285 | MS.gene87889:CDS | 20.0% |
| !! | TTTCAATAATCTTAAGGTAT+TGG | - | chr1.2:47203255-47203274 | None:intergenic | 20.0% |
| !! | TTTGTCAAATTGTAAAGTTA+AGG | - | chr1.2:47203092-47203111 | None:intergenic | 20.0% |
| !!! | CAAGTTTTTCAATAATCTTA+AGG | - | chr1.2:47203261-47203280 | None:intergenic | 20.0% |
| !!! | TTATGATAACTTTTCTACTA+TGG | + | chr1.2:47202940-47202959 | MS.gene87889:CDS | 20.0% |
| ! | ACTATGATGAATTGCAAAAA+AGG | + | chr1.2:47203413-47203432 | MS.gene87889:CDS | 25.0% |
| ! | AGTAATGAAATTAAGTGCTT+TGG | + | chr1.2:47203907-47203926 | MS.gene87889:CDS | 25.0% |
| ! | ATGAAATTAGAGAGATGAAA+AGG | + | chr1.2:47203461-47203480 | MS.gene87889:CDS | 25.0% |
| ! | CTATGATGAATTGCAAAAAA+GGG | + | chr1.2:47203414-47203433 | MS.gene87889:CDS | 25.0% |
| ! | GGAAGATTAATGTTTGTTTA+AGG | + | chr1.2:47203704-47203723 | MS.gene87889:CDS | 25.0% |
| ! | GTAATGAAATTAAGTGCTTT+GGG | + | chr1.2:47203908-47203927 | MS.gene87889:CDS | 25.0% |
| ! | TGAAATTAGAGAGATGAAAA+GGG | + | chr1.2:47203462-47203481 | MS.gene87889:CDS | 25.0% |
| ! | TTGAAGAACTTATTTACTTG+AGG | + | chr1.2:47203683-47203702 | MS.gene87889:CDS | 25.0% |
| !! | TTTAATCAACTTTCCCTTTT+TGG | - | chr1.2:47203827-47203846 | None:intergenic | 25.0% |
| AAGAACACTACTATCTATAG+AGG | - | chr1.2:47202993-47203012 | None:intergenic | 30.0% | |
| AAGGTTAAAGTAAAACCAGA+AGG | + | chr1.2:47203877-47203896 | MS.gene87889:CDS | 30.0% | |
| GAAGCTACTTAAGAAATCAA+AGG | + | chr1.2:47203324-47203343 | MS.gene87889:CDS | 30.0% | |
| TAAAAAGCTGAAAAATTGGG+TGG | + | chr1.2:47203843-47203862 | MS.gene87889:CDS | 30.0% | |
| TACTATGGAAGATGAAGAAA+AGG | + | chr1.2:47202955-47202974 | MS.gene87889:CDS | 30.0% | |
| TATGAAGGAAGATTACAACA+AGG | + | chr1.2:47203615-47203634 | MS.gene87889:CDS | 30.0% | |
| TCATAGAAACAGAAACTATG+AGG | + | chr1.2:47203212-47203231 | MS.gene87889:CDS | 30.0% | |
| TGAATCAAGAGATGTTATGA+AGG | + | chr1.2:47203600-47203619 | MS.gene87889:CDS | 30.0% | |
| ! | AGTATTGGAAATCAGAGAAT+AGG | + | chr1.2:47203281-47203300 | MS.gene87889:CDS | 30.0% |
| CAACAAGGTTCTGTATGAAT+TGG | + | chr1.2:47203630-47203649 | MS.gene87889:CDS | 35.0% | |
| CATCAAAATGTGTCTTGCAT+TGG | + | chr1.2:47203769-47203788 | MS.gene87889:CDS | 35.0% | |
| GGCTCAATCTCATTTGATTA+AGG | + | chr1.2:47203345-47203364 | MS.gene87889:CDS | 35.0% | |
| TGAATTGGAGCAAGTTAAGA+AGG | + | chr1.2:47203645-47203664 | MS.gene87889:CDS | 35.0% | |
| TGCTTAAGAAGCAAGATTGA+AGG | + | chr1.2:47203046-47203065 | MS.gene87889:CDS | 35.0% | |
| ! | ACATCATGAGAATTGACTTC+AGG | - | chr1.2:47202902-47202921 | None:intergenic | 35.0% |
| ! | AGTGTTCTTTCTGAGAGATT+TGG | + | chr1.2:47203004-47203023 | MS.gene87889:CDS | 35.0% |
| ! | ATCATGAGAATTGACTTCAG+GGG | - | chr1.2:47202900-47202919 | None:intergenic | 35.0% |
| ! | ATGAGAGTCAGTTTCTGAAA+AGG | - | chr1.2:47202876-47202895 | None:intergenic | 35.0% |
| ! | CAAGCTTTGATGATCAAAGA+GGG | + | chr1.2:47203370-47203389 | MS.gene87889:CDS | 35.0% |
| ! | CATCATGAGAATTGACTTCA+GGG | - | chr1.2:47202901-47202920 | None:intergenic | 35.0% |
| ! | TCTTTCTGAGAGATTTGGTT+TGG | + | chr1.2:47203009-47203028 | MS.gene87889:CDS | 35.0% |
| !! | CCTTCTTCTAGTTCCAAAAA+GGG | + | chr1.2:47203811-47203830 | MS.gene87889:CDS | 35.0% |
| !! | TCATTACTGCTTTTACCTTC+TGG | - | chr1.2:47203895-47203914 | None:intergenic | 35.0% |
| !! | TCCTTCTTCTAGTTCCAAAA+AGG | + | chr1.2:47203810-47203829 | MS.gene87889:CDS | 35.0% |
| !! | TGCATTGGTTCTTCATTTCA+TGG | + | chr1.2:47203784-47203803 | MS.gene87889:CDS | 35.0% |
| AAGCCTATTATCCTCAAAGC+TGG | + | chr1.2:47202773-47202792 | MS.gene87889:CDS | 40.0% | |
| AAGCTGAAAAATTGGGTGGA+TGG | + | chr1.2:47203847-47203866 | MS.gene87889:CDS | 40.0% | |
| CCAGCAAAAGAAACAGCTAT+AGG | - | chr1.2:47202803-47202822 | None:intergenic | 40.0% | |
| CTTGAGACATCAGAAGAACA+TGG | + | chr1.2:47203529-47203548 | MS.gene87889:CDS | 40.0% | |
| TAAGCACTAGAGCAAAACCT+TGG | - | chr1.2:47203955-47203974 | None:intergenic | 40.0% | |
| TAGAGAGATGAAAAGGGTCT+TGG | + | chr1.2:47203468-47203487 | MS.gene87889:CDS | 40.0% | |
| ! | GCAAGCTTTGATGATCAAAG+AGG | + | chr1.2:47203369-47203388 | MS.gene87889:CDS | 40.0% |
| !! | ACACCAGCTTTGAGGATAAT+AGG | - | chr1.2:47202779-47202798 | None:intergenic | 40.0% |
| !! | TTGCTGGTTTGATCTATGCA+TGG | + | chr1.2:47202816-47202835 | MS.gene87889:CDS | 40.0% |
| !!! | CCCTTTTTGGAACTAGAAGA+AGG | - | chr1.2:47203814-47203833 | None:intergenic | 40.0% |
| !!! | CCTATAGCTGTTTCTTTTGC+TGG | + | chr1.2:47202800-47202819 | MS.gene87889:CDS | 40.0% |
| CTATAGGAACACCAGCTTTG+AGG | - | chr1.2:47202787-47202806 | None:intergenic | 45.0% | |
| ATTCTGCTCCAGCTACTCCA+AGG | + | chr1.2:47203935-47203954 | MS.gene87889:CDS | 50.0% | |
| GAGCAAAACCTTGGAGTAGC+TGG | - | chr1.2:47203946-47203965 | None:intergenic | 50.0% | |
| TTGGGTGGATGGCAGTGAAA+AGG | + | chr1.2:47203858-47203877 | MS.gene87889:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 47202740 | 47203975 | 47202740 | ID=MS.gene87889 |
| chr1.2 | mRNA | 47202740 | 47203975 | 47202740 | ID=MS.gene87889.t1;Parent=MS.gene87889 |
| chr1.2 | exon | 47202740 | 47203975 | 47202740 | ID=MS.gene87889.t1.exon1;Parent=MS.gene87889.t1 |
| chr1.2 | CDS | 47202740 | 47203975 | 47202740 | ID=cds.MS.gene87889.t1;Parent=MS.gene87889.t1 |
| Gene Sequence |
| Protein sequence |
