Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87963.t1 | XP_003618953.1 | 93.2 | 236 | 8 | 1 | 1 | 236 | 1 | 228 | 2.30E-116 | 428.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87963.t1 | B7FJV5 | 93.2 | 236 | 8 | 1 | 1 | 236 | 1 | 228 | 1.6e-116 | 428.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050508 | MS.gene87963 | 0.876173 | 1.72E-68 | -1.69E-46 |
| MS.gene059710 | MS.gene87963 | 0.85709 | 2.07E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87963.t1 | MTR_6g029250 | 93.220 | 236 | 8 | 1 | 1 | 236 | 1 | 228 | 2.30e-161 | 446 |
| MS.gene87963.t1 | MTR_7g085790 | 48.846 | 260 | 96 | 9 | 1 | 233 | 1 | 250 | 3.03e-66 | 205 |
| MS.gene87963.t1 | MTR_7g085790 | 45.200 | 250 | 81 | 10 | 1 | 233 | 1 | 211 | 2.00e-54 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87963.t1 | AT3G03870 | 36.162 | 271 | 128 | 14 | 1 | 233 | 1 | 264 | 4.21e-31 | 115 |
| MS.gene87963.t1 | AT5G18130 | 42.775 | 173 | 61 | 7 | 91 | 233 | 114 | 278 | 1.64e-30 | 114 |
| MS.gene87963.t1 | AT3G03870 | 31.356 | 236 | 118 | 13 | 1 | 199 | 1 | 229 | 4.14e-14 | 69.7 |
| MS.gene87963.t1 | AT5G18130 | 36.170 | 141 | 53 | 6 | 91 | 202 | 114 | 246 | 1.23e-13 | 68.6 |
Find 37 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATAGTGGTTTCAATTTA+TGG | 0.117037 | 6.1:+10890470 | MS.gene87963:CDS |
| TCTTTCTCAATAATCATTCT+TGG | 0.230298 | 6.1:-10890403 | None:intergenic |
| GTTATGCGAGCTCTTGTTAT+AGG | 0.236659 | 6.1:-10891479 | None:intergenic |
| AAGATTAAGGAAAATGAAAT+TGG | 0.238321 | 6.1:+10890325 | MS.gene87963:CDS |
| GTAACACCTCAAATGATGTT+AGG | 0.278915 | 6.1:+10890233 | MS.gene87963:CDS |
| GTTGGTGCTATTTGCACTTT+TGG | 0.279828 | 6.1:+10890511 | MS.gene87963:CDS |
| ATTATTGAGAAAGATGATTT+TGG | 0.285258 | 6.1:+10890412 | MS.gene87963:CDS |
| TTATGGAAGTGGAGTCTTAC+TGG | 0.287880 | 6.1:+10890487 | MS.gene87963:CDS |
| CCTTGTCATCAGCATAGATT+TGG | 0.343870 | 6.1:-10890615 | None:intergenic |
| TTACCAAGTTTGAGGTCTAA+TGG | 0.382258 | 6.1:-10890214 | None:intergenic |
| GTGGTTTCAATTTATGGAAG+TGG | 0.411257 | 6.1:+10890476 | MS.gene87963:CDS |
| GAAGCAATTTCTGTTGTAAG+AGG | 0.414220 | 6.1:+10891452 | MS.gene87963:CDS |
| GGTCTAATGGAATTTGTAAA+GGG | 0.426783 | 6.1:-10890201 | None:intergenic |
| TGGGAAGAAGAGAACAATAG+TGG | 0.445840 | 6.1:+10890457 | MS.gene87963:CDS |
| TTGAGGTGTTACCAAGTTTG+AGG | 0.452496 | 6.1:-10890222 | None:intergenic |
| TTGAACCCTAACATCATTTG+AGG | 0.461171 | 6.1:-10890239 | None:intergenic |
| AGGTCTAATGGAATTTGTAA+AGG | 0.463571 | 6.1:-10890202 | None:intergenic |
| TCTTTGCTACTACTTGACTT+TGG | 0.472978 | 6.1:-10890367 | None:intergenic |
| TGGAGATGGATCTTGATGAA+TGG | 0.483700 | 6.1:+10890041 | None:intergenic |
| GAATTTGTAAAGGGACAAGT+TGG | 0.486114 | 6.1:-10890192 | None:intergenic |
| GGAGATGGATCTTGATGAAT+GGG | 0.498676 | 6.1:+10890042 | None:intergenic |
| TAACACCTCAAATGATGTTA+GGG | 0.499406 | 6.1:+10890234 | MS.gene87963:CDS |
| TTCAGAAGGATCAGAAGAAT+AGG | 0.499980 | 6.1:+10890590 | MS.gene87963:CDS |
| ATTCCATTAGACCTCAAACT+TGG | 0.502699 | 6.1:+10890211 | MS.gene87963:CDS |
| CAAAGAAGAGGATTCAAGTT+GGG | 0.511674 | 6.1:+10890438 | MS.gene87963:CDS |
| TATATTGATTTCAATGAAGA+TGG | 0.511704 | 6.1:+10890085 | MS.gene87963:CDS |
| AAGCAATTTCTGTTGTAAGA+GGG | 0.518875 | 6.1:+10891453 | MS.gene87963:CDS |
| AGCAATTTCTGTTGTAAGAG+GGG | 0.561387 | 6.1:+10891454 | MS.gene87963:CDS |
| ATTTCAGAGGATTAAGCAAG+TGG | 0.575069 | 6.1:+10891406 | MS.gene87963:intron |
| AGAGCTCGCATAACAGTTGG+TGG | 0.577100 | 6.1:+10891488 | MS.gene87963:CDS |
| ACAAAGAAGAGGATTCAAGT+TGG | 0.588072 | 6.1:+10890437 | MS.gene87963:CDS |
| CCAAATCTATGCTGATGACA+AGG | 0.589218 | 6.1:+10890615 | MS.gene87963:CDS |
| TGGGAATCAATCTCTAGTGA+TGG | 0.593054 | 6.1:+10890061 | MS.gene87963:CDS |
| AAGTGGAGTCTTACTGGTGT+TGG | 0.594782 | 6.1:+10890493 | MS.gene87963:CDS |
| ACAAGAGCTCGCATAACAGT+TGG | 0.598712 | 6.1:+10891485 | MS.gene87963:CDS |
| ATCAGAAAGTGCTTTAGACA+TGG | 0.600275 | 6.1:+10890135 | MS.gene87963:CDS |
| TGTTCTTTGGAAGCCAACAA+AGG | 0.602238 | 6.1:+10890560 | MS.gene87963:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTGATTAATAGTA+CGG | + | chr6.1:10890690-10890709 | MS.gene87963:intron | 10.0% |
| !! | GTTTAAATATTCAATTGATT+AGG | + | chr6.1:10891350-10891369 | MS.gene87963:intron | 15.0% |
| !!! | TATAATTTTTCATATTTCCA+AGG | - | chr6.1:10890856-10890875 | None:intergenic | 15.0% |
| !! | AAGATTAAGGAAAATGAAAT+TGG | + | chr6.1:10890325-10890344 | MS.gene87963:CDS | 20.0% |
| !! | ATAGACAAAATAAGTTTATG+TGG | + | chr6.1:10890873-10890892 | MS.gene87963:intron | 20.0% |
| !! | TAAACCATTTATACTAAATC+AGG | - | chr6.1:10890758-10890777 | None:intergenic | 20.0% |
| !! | TTTAGTATAGAATTATGTCA+TGG | + | chr6.1:10890799-10890818 | MS.gene87963:intron | 20.0% |
| !!! | ACAAGTTTTCTTTAAGATTA+AGG | + | chr6.1:10890312-10890331 | MS.gene87963:CDS | 20.0% |
| !!! | ATTATTGAGAAAGATGATTT+TGG | + | chr6.1:10890412-10890431 | MS.gene87963:CDS | 20.0% |
| !!! | TATATTGATTTCAATGAAGA+TGG | + | chr6.1:10890085-10890104 | MS.gene87963:CDS | 20.0% |
| !!! | TTTTACTCATTATGTTGTTA+AGG | + | chr6.1:10890903-10890922 | MS.gene87963:intron | 20.0% |
| ! | AAAAAAAGTTTCCTAGGAAA+AGG | + | chr6.1:10890112-10890131 | MS.gene87963:CDS | 25.0% |
| ! | ACAATAGTGGTTTCAATTTA+TGG | + | chr6.1:10890470-10890489 | MS.gene87963:CDS | 25.0% |
| ! | AGAACCTGATTTAGTATAAA+TGG | + | chr6.1:10890751-10890770 | MS.gene87963:intron | 25.0% |
| ! | ATACAGTAGTTCACTATATT+TGG | + | chr6.1:10891328-10891347 | MS.gene87963:intron | 25.0% |
| ! | GGTATTTCTTGAATTATAAG+TGG | - | chr6.1:10891233-10891252 | None:intergenic | 25.0% |
| ! | TCTTTCTCAATAATCATTCT+TGG | - | chr6.1:10890406-10890425 | None:intergenic | 25.0% |
| ! | TTGTATATTGAACTAAACCT+TGG | + | chr6.1:10891378-10891397 | MS.gene87963:intron | 25.0% |
| !! | ACAAGGTTAGTAAATTTTCT+TGG | + | chr6.1:10890632-10890651 | MS.gene87963:intron | 25.0% |
| !!! | ATGGTTTTAAATTGATCTTG+AGG | + | chr6.1:10890818-10890837 | MS.gene87963:intron | 25.0% |
| !!! | GTTAACACTTTTTTACTCTA+TGG | - | chr6.1:10891254-10891273 | None:intergenic | 25.0% |
| !!! | TCTATTTGTGTTTTGTTCTT+TGG | + | chr6.1:10890547-10890566 | MS.gene87963:CDS | 25.0% |
| !!! | TTTTTCGAATCAAAACAATC+AGG | + | chr6.1:10891169-10891188 | MS.gene87963:intron | 25.0% |
| AAGCAATTTCTGTTGTAAGA+GGG | + | chr6.1:10891453-10891472 | MS.gene87963:CDS | 30.0% | |
| ACAAAGGAAAAAACTTCAGA+AGG | + | chr6.1:10890576-10890595 | MS.gene87963:CDS | 30.0% | |
| AGGTCTAATGGAATTTGTAA+AGG | - | chr6.1:10890205-10890224 | None:intergenic | 30.0% | |
| GGTCTAATGGAATTTGTAAA+GGG | - | chr6.1:10890204-10890223 | None:intergenic | 30.0% | |
| TAACACCTCAAATGATGTTA+GGG | + | chr6.1:10890234-10890253 | MS.gene87963:CDS | 30.0% | |
| TTGTTAAGGTTGCAATTACA+TGG | + | chr6.1:10890917-10890936 | MS.gene87963:intron | 30.0% | |
| TTTCATATTTCCAAGGATGT+TGG | - | chr6.1:10890849-10890868 | None:intergenic | 30.0% | |
| ! | AAAGTGTTAACTTTGTGTCA+AGG | + | chr6.1:10891263-10891282 | MS.gene87963:intron | 30.0% |
| ! | TTGCAATTACATGGTAGTTT+TGG | + | chr6.1:10890926-10890945 | MS.gene87963:intron | 30.0% |
| !! | CTTAGTCATCTGATTTTGAT+CGG | + | chr6.1:10891075-10891094 | MS.gene87963:intron | 30.0% |
| !! | CTTTAGAGTTGGAGAATTTA+TGG | + | chr6.1:10890986-10891005 | MS.gene87963:intron | 30.0% |
| !!! | ACAAGTTGGTTTTGTTTTCT+TGG | - | chr6.1:10890181-10890200 | None:intergenic | 30.0% |
| !!! | TCTGAAGTTTTTTCCTTTGT+TGG | - | chr6.1:10890576-10890595 | None:intergenic | 30.0% |
| ACAAAGAAGAGGATTCAAGT+TGG | + | chr6.1:10890437-10890456 | MS.gene87963:CDS | 35.0% | |
| ACCTTGTTAGTTGATTGATC+TGG | + | chr6.1:10890960-10890979 | MS.gene87963:intron | 35.0% | |
| AGCAATTTCTGTTGTAAGAG+GGG | + | chr6.1:10891454-10891473 | MS.gene87963:CDS | 35.0% | |
| ATTCCATTAGACCTCAAACT+TGG | + | chr6.1:10890211-10890230 | MS.gene87963:CDS | 35.0% | |
| ATTTCAGAGGATTAAGCAAG+TGG | + | chr6.1:10891406-10891425 | MS.gene87963:intron | 35.0% | |
| GAAGCAATTTCTGTTGTAAG+AGG | + | chr6.1:10891452-10891471 | MS.gene87963:CDS | 35.0% | |
| GAATTTGTAAAGGGACAAGT+TGG | - | chr6.1:10890195-10890214 | None:intergenic | 35.0% | |
| GATCATCCAATCAAGATCTA+TGG | + | chr6.1:10891029-10891048 | MS.gene87963:intron | 35.0% | |
| GGTGAGAAAAAAAGTTTCCT+AGG | + | chr6.1:10890106-10890125 | MS.gene87963:CDS | 35.0% | |
| GTAACACCTCAAATGATGTT+AGG | + | chr6.1:10890233-10890252 | MS.gene87963:CDS | 35.0% | |
| GTGGTTTCAATTTATGGAAG+TGG | + | chr6.1:10890476-10890495 | MS.gene87963:CDS | 35.0% | |
| TCCAGATCAATCAACTAACA+AGG | - | chr6.1:10890964-10890983 | None:intergenic | 35.0% | |
| TCTTTGCTACTACTTGACTT+TGG | - | chr6.1:10890370-10890389 | None:intergenic | 35.0% | |
| TGTTAACTTTGTGTCAAGGA+TGG | + | chr6.1:10891267-10891286 | MS.gene87963:intron | 35.0% | |
| TTCAGAAGGATCAGAAGAAT+AGG | + | chr6.1:10890590-10890609 | MS.gene87963:CDS | 35.0% | |
| TTGAACCCTAACATCATTTG+AGG | - | chr6.1:10890242-10890261 | None:intergenic | 35.0% | |
| ! | CAAAGAAGAGGATTCAAGTT+GGG | + | chr6.1:10890438-10890457 | MS.gene87963:CDS | 35.0% |
| ! | CTTGTGACACATTTTCTTCT+TGG | - | chr6.1:10890297-10890316 | None:intergenic | 35.0% |
| ! | TTACCAAGTTTGAGGTCTAA+TGG | - | chr6.1:10890217-10890236 | None:intergenic | 35.0% |
| !! | ATCAGAAAGTGCTTTAGACA+TGG | + | chr6.1:10890135-10890154 | MS.gene87963:CDS | 35.0% |
| !! | ATCCTCTGAAATCAAAACCA+AGG | - | chr6.1:10891398-10891417 | None:intergenic | 35.0% |
| !! | TGATCTGGATTCTTTAGAGT+TGG | + | chr6.1:10890975-10890994 | MS.gene87963:intron | 35.0% |
| !! | TGATTTTGGTGACAAAGAAG+AGG | + | chr6.1:10890426-10890445 | MS.gene87963:CDS | 35.0% |
| !!! | AACCTTGGTTTTGATTTCAG+AGG | + | chr6.1:10891393-10891412 | MS.gene87963:intron | 35.0% |
| !!! | AGTTGGTTTTGTTTTCTTGG+TGG | - | chr6.1:10890178-10890197 | None:intergenic | 35.0% |
| !!! | TTTTGTTTTCTTGGTGGTGA+AGG | - | chr6.1:10890172-10890191 | None:intergenic | 35.0% |
| ATTGAATCTCAGTCGATCGT+GGG | + | chr6.1:10891192-10891211 | MS.gene87963:intron | 40.0% | |
| CCAAATCTATGCTGATGACA+AGG | + | chr6.1:10890615-10890634 | MS.gene87963:CDS | 40.0% | |
| CCTTGTCATCAGCATAGATT+TGG | - | chr6.1:10890618-10890637 | None:intergenic | 40.0% | |
| CGAATGCCATAGATCTTGAT+TGG | - | chr6.1:10891038-10891057 | None:intergenic | 40.0% | |
| TGGGAAGAAGAGAACAATAG+TGG | + | chr6.1:10890457-10890476 | MS.gene87963:CDS | 40.0% | |
| TGGGAATCAATCTCTAGTGA+TGG | + | chr6.1:10890061-10890080 | MS.gene87963:CDS | 40.0% | |
| TTATGGAAGTGGAGTCTTAC+TGG | + | chr6.1:10890487-10890506 | MS.gene87963:CDS | 40.0% | |
| ! | CACTTTCTGATCCTTTTCCT+AGG | - | chr6.1:10890126-10890145 | None:intergenic | 40.0% |
| ! | GTTATGCGAGCTCTTGTTAT+AGG | - | chr6.1:10891482-10891501 | None:intergenic | 40.0% |
| ! | TGTTCTTTGGAAGCCAACAA+AGG | + | chr6.1:10890560-10890579 | MS.gene87963:CDS | 40.0% |
| ! | TTGAGGTGTTACCAAGTTTG+AGG | - | chr6.1:10890225-10890244 | None:intergenic | 40.0% |
| !!! | GTTGGTGCTATTTGCACTTT+TGG | + | chr6.1:10890511-10890530 | MS.gene87963:CDS | 40.0% |
| AAGTGGAGTCTTACTGGTGT+TGG | + | chr6.1:10890493-10890512 | MS.gene87963:CDS | 45.0% | |
| ACAAGAGCTCGCATAACAGT+TGG | + | chr6.1:10891485-10891504 | MS.gene87963:CDS | 45.0% | |
| GATTGAATCTCAGTCGATCG+TGG | + | chr6.1:10891191-10891210 | MS.gene87963:intron | 45.0% | |
| TGAGGTTACACCAACATCCT+TGG | + | chr6.1:10890836-10890855 | MS.gene87963:intron | 45.0% | |
| AGAGCTCGCATAACAGTTGG+TGG | + | chr6.1:10891488-10891507 | MS.gene87963:CDS | 50.0% | |
| GGAGTCATCGTTAGCATGTC+TGG | + | chr6.1:10891007-10891026 | MS.gene87963:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 10890046 | 10891532 | 10890046 | ID=MS.gene87963 |
| chr6.1 | mRNA | 10890046 | 10891532 | 10890046 | ID=MS.gene87963.t1;Parent=MS.gene87963 |
| chr6.1 | exon | 10890046 | 10890636 | 10890046 | ID=MS.gene87963.t1.exon1;Parent=MS.gene87963.t1 |
| chr6.1 | CDS | 10890046 | 10890636 | 10890046 | ID=cds.MS.gene87963.t1;Parent=MS.gene87963.t1 |
| chr6.1 | exon | 10891413 | 10891532 | 10891413 | ID=MS.gene87963.t1.exon2;Parent=MS.gene87963.t1 |
| chr6.1 | CDS | 10891413 | 10891532 | 10891413 | ID=cds.MS.gene87963.t1;Parent=MS.gene87963.t1 |
| Gene Sequence |
| Protein sequence |
