Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88350.t1 | XP_013453884.1 | 85.5 | 55 | 8 | 0 | 1 | 55 | 238 | 292 | 2.40E-17 | 97.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88350.t1 | Q8W4Y5 | 61.8 | 55 | 21 | 0 | 1 | 55 | 235 | 289 | 4.4e-12 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88350.t1 | A0A072UQ04 | 85.5 | 55 | 8 | 0 | 1 | 55 | 238 | 292 | 1.7e-17 | 97.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058515 | MS.gene88350 | 0.807148 | 5.82E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTGTTCTTAACATTTAT+TGG | 0.179322 | 5.3:+40947997 | MS.gene88350:CDS |
| ACTGGTTGCCACCTTGAAAT+TGG | 0.253838 | 5.3:+40947914 | MS.gene88350:CDS |
| CCTTGAAATTGGAAAATCAT+TGG | 0.362965 | 5.3:+40947925 | MS.gene88350:CDS |
| TTGGAAAATCATTGGGTGAA+TGG | 0.396407 | 5.3:+40947933 | MS.gene88350:CDS |
| CTTGAAATTGGAAAATCATT+GGG | 0.421846 | 5.3:+40947926 | MS.gene88350:CDS |
| ATTGGTGGGTGCTGATTCTT+CGG | 0.444061 | 5.3:+40948015 | MS.gene88350:CDS |
| TCTTTGTTGCAGCTATGAAC+TGG | 0.456363 | 5.3:+40947896 | None:intergenic |
| TTGTTCTTAACATTTATTGG+TGG | 0.510641 | 5.3:+40948000 | MS.gene88350:CDS |
| GCTGATTCTTCGGATGCTGT+TGG | 0.512355 | 5.3:+40948025 | MS.gene88350:CDS |
| GTTAGTGAAGATATACGATC+TGG | 0.513493 | 5.3:+40948070 | MS.gene88350:CDS |
| TGTTCTTAACATTTATTGGT+GGG | 0.530572 | 5.3:+40948001 | MS.gene88350:CDS |
| GTTGGTCAAATTCGAGCAAG+AGG | 0.595938 | 5.3:+40948043 | MS.gene88350:CDS |
| TTGGTCAAATTCGAGCAAGA+GGG | 0.645930 | 5.3:+40948044 | MS.gene88350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTGAAATTGGAAAATCATT+GGG | + | chr5.3:40947926-40947945 | MS.gene88350:CDS | 25.0% |
| ! | TGCTTGTTCTTAACATTTAT+TGG | + | chr5.3:40947997-40948016 | MS.gene88350:CDS | 25.0% |
| ! | TGTTCTTAACATTTATTGGT+GGG | + | chr5.3:40948001-40948020 | MS.gene88350:CDS | 25.0% |
| ! | TTGTTCTTAACATTTATTGG+TGG | + | chr5.3:40948000-40948019 | MS.gene88350:CDS | 25.0% |
| CCTTGAAATTGGAAAATCAT+TGG | + | chr5.3:40947925-40947944 | MS.gene88350:CDS | 30.0% | |
| ! | ATGATTTTCCAATTTCAAGG+TGG | - | chr5.3:40947925-40947944 | None:intergenic | 30.0% |
| ! | CCAATGATTTTCCAATTTCA+AGG | - | chr5.3:40947928-40947947 | None:intergenic | 30.0% |
| TTGGAAAATCATTGGGTGAA+TGG | + | chr5.3:40947933-40947952 | MS.gene88350:CDS | 35.0% | |
| TTGGTCAAATTCGAGCAAGA+GGG | + | chr5.3:40948044-40948063 | MS.gene88350:CDS | 40.0% | |
| ACTGGTTGCCACCTTGAAAT+TGG | + | chr5.3:40947914-40947933 | MS.gene88350:CDS | 45.0% | |
| GTTGGTCAAATTCGAGCAAG+AGG | + | chr5.3:40948043-40948062 | MS.gene88350:CDS | 45.0% | |
| !! | ATTGGTGGGTGCTGATTCTT+CGG | + | chr5.3:40948015-40948034 | MS.gene88350:CDS | 45.0% |
| ! | GCTGATTCTTCGGATGCTGT+TGG | + | chr5.3:40948025-40948044 | MS.gene88350:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 40947908 | 40948091 | 40947908 | ID=MS.gene88350 |
| chr5.3 | mRNA | 40947908 | 40948091 | 40947908 | ID=MS.gene88350.t1;Parent=MS.gene88350 |
| chr5.3 | exon | 40947908 | 40948091 | 40947908 | ID=MS.gene88350.t1.exon1;Parent=MS.gene88350.t1 |
| chr5.3 | CDS | 40947908 | 40948091 | 40947908 | ID=cds.MS.gene88350.t1;Parent=MS.gene88350.t1 |
| Gene Sequence |
| Protein sequence |
