Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88674.t1 | AES92327.2 | 96.7 | 547 | 18 | 0 | 1 | 547 | 93 | 639 | 0.00E+00 | 1097 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88674.t1 | Q9XI84 | 24.1 | 278 | 192 | 9 | 144 | 410 | 47 | 316 | 1.2e-12 | 76.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88674.t1 | G7JVG8 | 96.7 | 547 | 18 | 0 | 1 | 547 | 93 | 639 | 0.0e+00 | 1097.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88674.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056228 | MS.gene88674 | 0.802591 | 5.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88674.t1 | MTR_4g128240 | 96.709 | 547 | 18 | 0 | 1 | 547 | 93 | 639 | 0.0 | 1100 |
| MS.gene88674.t1 | MTR_3g082690 | 22.596 | 416 | 268 | 13 | 142 | 518 | 63 | 463 | 3.44e-18 | 87.8 |
| MS.gene88674.t1 | MTR_4g082305 | 23.154 | 298 | 202 | 11 | 124 | 410 | 42 | 323 | 1.54e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88674.t1 | AT2G18850 | 51.873 | 534 | 251 | 4 | 5 | 537 | 10 | 538 | 0.0 | 553 |
| MS.gene88674.t1 | AT2G18850 | 51.498 | 534 | 246 | 5 | 5 | 537 | 10 | 531 | 0.0 | 545 |
| MS.gene88674.t1 | AT3G07670 | 23.629 | 474 | 311 | 18 | 82 | 520 | 15 | 472 | 1.70e-20 | 95.1 |
| MS.gene88674.t1 | AT3G07670 | 24.843 | 318 | 199 | 11 | 234 | 520 | 26 | 334 | 5.59e-16 | 80.1 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATTTAATTTCTGATTT+TGG | 0.061221 | 8.1:+4564280 | MS.gene88674:CDS |
| TGGTCTTCCCTCTCCATTAT+TGG | 0.251856 | 8.1:+4572765 | MS.gene88674:CDS |
| ATACATTATCTTCATGCTAT+TGG | 0.253277 | 8.1:-4571752 | None:intergenic |
| GTTTCAACTTCTTGCTGTAC+TGG | 0.267684 | 8.1:+4573737 | MS.gene88674:CDS |
| TAATTTGCAGGATTGAGTTT+TGG | 0.273161 | 8.1:+4567812 | MS.gene88674:intron |
| GTACTCCCTTCATATTGTTT+TGG | 0.278110 | 8.1:-4564306 | None:intergenic |
| CCGTATGATGTCATTCCTTT+AGG | 0.285448 | 8.1:+4572494 | MS.gene88674:CDS |
| GAAAATGAGCTAGAAGTTCT+TGG | 0.287372 | 8.1:+4573184 | MS.gene88674:CDS |
| CTGAGACAATATTGCTGTTA+TGG | 0.304721 | 8.1:+4565272 | MS.gene88674:CDS |
| GAGAGCCTCCAACTCATTCC+TGG | 0.355681 | 8.1:-4564170 | None:intergenic |
| TCAGAAATTACTACGATGTA+AGG | 0.359589 | 8.1:+4563739 | MS.gene88674:intron |
| AATCACAGCATATTCTATTA+TGG | 0.363717 | 8.1:+4572745 | MS.gene88674:CDS |
| AGTGCTCCTCAGGACCTTCT+TGG | 0.369830 | 8.1:+4563792 | MS.gene88674:CDS |
| ACATGTTTGATACCTCTTGC+TGG | 0.371762 | 8.1:+4571793 | MS.gene88674:CDS |
| TCCACAGTTGCAAGGAAACT+TGG | 0.382837 | 8.1:+4573162 | MS.gene88674:intron |
| TGACGACATGATGGACAGTA+TGG | 0.401765 | 8.1:+4573225 | MS.gene88674:CDS |
| ATCTCTGGAGGAAATATATC+AGG | 0.406816 | 8.1:-4571685 | None:intergenic |
| CTCCATTATTGGATCATCTT+CGG | 0.411384 | 8.1:+4572776 | MS.gene88674:CDS |
| CTATCAAGACCATGCCGATC+TGG | 0.413805 | 8.1:+4572389 | MS.gene88674:CDS |
| ATAGAATATGCTGTGATTGT+TGG | 0.416388 | 8.1:-4572740 | None:intergenic |
| CACCATGTGAGTCGTCCATT+TGG | 0.419396 | 8.1:-4572701 | None:intergenic |
| TGCCCACATATTACACACTA+TGG | 0.422578 | 8.1:+4572329 | MS.gene88674:CDS |
| TTCTCCCAAGTGTAAATCTC+TGG | 0.423678 | 8.1:-4571700 | None:intergenic |
| CTCCGAAGATGATCCAATAA+TGG | 0.426430 | 8.1:-4572778 | None:intergenic |
| AATTTCAATGAATTTGTTGA+AGG | 0.427682 | 8.1:-4572362 | None:intergenic |
| GCTTGTGCTTGAAACTGATA+TGG | 0.430090 | 8.1:+4564639 | MS.gene88674:CDS |
| AGATGTTGAAGGAGCAGGCC+GGG | 0.432091 | 8.1:+4564540 | MS.gene88674:intron |
| GCAGGCCGGGGAATGATAGC+AGG | 0.433260 | 8.1:+4564553 | MS.gene88674:CDS |
| AAAGTCTTTGTTTGCATCAC+AGG | 0.436089 | 8.1:-4572811 | None:intergenic |
| TTACTACGATGTAAGGGGTT+TGG | 0.439522 | 8.1:+4563746 | MS.gene88674:CDS |
| TTTCAACTTCTTGCTGTACT+GGG | 0.444329 | 8.1:+4573738 | MS.gene88674:CDS |
| CTCACATGGTGCGTGGTACC+TGG | 0.452049 | 8.1:+4572713 | MS.gene88674:CDS |
| TCCTTTCTTTGATGAAAAGA+AGG | 0.455845 | 8.1:+4562824 | MS.gene88674:CDS |
| GAATGAACTGCACAAGTGTT+TGG | 0.459339 | 8.1:+4573775 | MS.gene88674:CDS |
| TGTTTGGCTGAGGACATTGT+AGG | 0.459596 | 8.1:+4573791 | MS.gene88674:CDS |
| ACCCTTGCAGGATGTTTGTA+TGG | 0.460432 | 8.1:-4564243 | None:intergenic |
| TTGGTTCCTGCTTTGTGCAA+TGG | 0.460579 | 8.1:+4571658 | MS.gene88674:CDS |
| TTAATCTACACAGATGTTGA+AGG | 0.462913 | 8.1:+4564529 | MS.gene88674:intron |
| ATACATCTCAAGTGCTCCTC+AGG | 0.464633 | 8.1:+4563782 | MS.gene88674:CDS |
| AGGATAAACCTGTGTCCAAA+TGG | 0.465266 | 8.1:+4572686 | MS.gene88674:CDS |
| TTGAGATGTATCCATTCCTT+GGG | 0.465915 | 8.1:-4563770 | None:intergenic |
| CTTGAGATGTATCCATTCCT+TGG | 0.467813 | 8.1:-4563771 | None:intergenic |
| GTGAAGTCGATCTTGATAAC+AGG | 0.473430 | 8.1:+4573248 | MS.gene88674:CDS |
| TTCTGATGTTGATTCTATTG+AGG | 0.474846 | 8.1:+4572666 | MS.gene88674:CDS |
| TCAGTATCGTCTCTTGAAAG+AGG | 0.476597 | 8.1:+4565234 | MS.gene88674:intron |
| GAGAAGAACACCTGATTGAG+TGG | 0.477724 | 8.1:+4564353 | MS.gene88674:CDS |
| GCAGGGAAAGATCTAAAAGT+TGG | 0.483249 | 8.1:+4564571 | MS.gene88674:CDS |
| TGGGGACCAAAACAATATGA+AGG | 0.487327 | 8.1:+4564300 | MS.gene88674:CDS |
| GAAGAATGCTGCTTAAGCTA+TGG | 0.488333 | 8.1:+4572413 | MS.gene88674:CDS |
| CGTCTCTTGAAAGAGGTTGA+TGG | 0.489397 | 8.1:+4565241 | MS.gene88674:CDS |
| AGCAAGAAGTTGAAACAGAA+TGG | 0.489908 | 8.1:-4573730 | None:intergenic |
| GAAGCGATTACAATGCTAGA+TGG | 0.490904 | 8.1:+4567839 | MS.gene88674:CDS |
| CCTAAAGGAATGACATCATA+CGG | 0.505016 | 8.1:-4572494 | None:intergenic |
| GCTGTGATTGTTGGAGAGCC+AGG | 0.506463 | 8.1:-4572731 | None:intergenic |
| CAGCCCCAGGAATGAGTTGG+AGG | 0.515961 | 8.1:+4564165 | MS.gene88674:CDS |
| AGAGCCTCCAACTCATTCCT+GGG | 0.519139 | 8.1:-4564169 | None:intergenic |
| TCCTCCAGAGATTTACACTT+GGG | 0.521467 | 8.1:+4571696 | MS.gene88674:CDS |
| TACACAGATGTTGAAGGAGC+AGG | 0.521506 | 8.1:+4564535 | MS.gene88674:intron |
| CGTGTTCATTATCATTTCTG+AGG | 0.525279 | 8.1:+4564615 | MS.gene88674:CDS |
| AAAAGCCATGGTGTAAAGAC+AGG | 0.525288 | 8.1:+4564379 | MS.gene88674:CDS |
| ACGATATCATTCATCCAAGA+AGG | 0.525319 | 8.1:-4563806 | None:intergenic |
| AAATGTAATAAAATGAGAGC+TGG | 0.529710 | 8.1:-4572444 | None:intergenic |
| GGAAAACCATTGCACAAAGC+AGG | 0.530912 | 8.1:-4571664 | None:intergenic |
| CAGAAATTACTACGATGTAA+GGG | 0.531459 | 8.1:+4563740 | MS.gene88674:intron |
| ATGTAAGGGGTTTGGTCCCA+AGG | 0.532723 | 8.1:+4563754 | MS.gene88674:CDS |
| AGGGGTTTGGTCCCAAGGAA+TGG | 0.533877 | 8.1:+4563759 | MS.gene88674:CDS |
| CAGATGTTGAAGGAGCAGGC+CGG | 0.534056 | 8.1:+4564539 | MS.gene88674:intron |
| CTTTCCCTGCTATCATTCCC+CGG | 0.534319 | 8.1:-4564558 | None:intergenic |
| AAGAATGCTGCTTAAGCTAT+GGG | 0.536764 | 8.1:+4572414 | MS.gene88674:CDS |
| TTCTCAGAGAAAGCTACACC+TGG | 0.537382 | 8.1:+4573660 | MS.gene88674:intron |
| GCAAGAAGTTGAAACAGAAT+GGG | 0.537385 | 8.1:-4573729 | None:intergenic |
| TTACAGCCCCAGGAATGAGT+TGG | 0.537964 | 8.1:+4564162 | MS.gene88674:CDS |
| AGCAGCATTCTTCTCCAGAT+CGG | 0.542200 | 8.1:-4572403 | None:intergenic |
| ACAACTAGAAAGTGATTTGT+CGG | 0.544339 | 8.1:-4564212 | None:intergenic |
| GCACTCCTCGTCACTATCCA+TGG | 0.550146 | 8.1:-4562772 | None:intergenic |
| AGAGATAATGCAATCAAGAC+AGG | 0.553032 | 8.1:+4567877 | MS.gene88674:CDS |
| AGACATCTTTGACGACATGA+TGG | 0.557106 | 8.1:+4573216 | MS.gene88674:CDS |
| TTCCATACAAACATCCTGCA+AGG | 0.560101 | 8.1:+4564241 | MS.gene88674:CDS |
| TGAGTCGTCCATTTGGACAC+AGG | 0.564379 | 8.1:-4572694 | None:intergenic |
| CATTATCTCTTCAAACAGCA+AGG | 0.565535 | 8.1:-4567864 | None:intergenic |
| GCATGCTCAGTATGATGAGT+TGG | 0.574500 | 8.1:+4571639 | MS.gene88674:CDS |
| AGATGATCCAATAATGGAGA+GGG | 0.579934 | 8.1:-4572772 | None:intergenic |
| TTCCTCCAGAGATTTACACT+TGG | 0.582164 | 8.1:+4571695 | MS.gene88674:CDS |
| TTGCCATAGTGTGTAATATG+TGG | 0.582672 | 8.1:-4572332 | None:intergenic |
| AGAAGAACACCTGATTGAGT+GGG | 0.587488 | 8.1:+4564354 | MS.gene88674:CDS |
| AGAAATTACTACGATGTAAG+GGG | 0.589340 | 8.1:+4563741 | MS.gene88674:intron |
| GGGGACCAAAACAATATGAA+GGG | 0.590986 | 8.1:+4564301 | MS.gene88674:CDS |
| CTCAGAAATGATAATGAACA+CGG | 0.600350 | 8.1:-4564614 | None:intergenic |
| TCCATACAAACATCCTGCAA+GGG | 0.600641 | 8.1:+4564242 | MS.gene88674:CDS |
| ATGAAGATAATGTATTCAGA+TGG | 0.601114 | 8.1:+4571760 | MS.gene88674:CDS |
| GATGTTGAAGGAGCAGGCCG+GGG | 0.613770 | 8.1:+4564541 | MS.gene88674:intron |
| TGATTAAGAAAGCCAGCAAG+AGG | 0.615019 | 8.1:-4571805 | None:intergenic |
| TCCCAAGTGTAAATCTCTGG+AGG | 0.620554 | 8.1:-4571697 | None:intergenic |
| TTAATCCTGTCTTTACACCA+TGG | 0.622253 | 8.1:-4564384 | None:intergenic |
| CATTCTTCTCCAGATCGGCA+TGG | 0.630402 | 8.1:-4572398 | None:intergenic |
| GAGCCTCCAACTCATTCCTG+GGG | 0.630829 | 8.1:-4564168 | None:intergenic |
| TGCTGAAGACGATGCCTCGG+TGG | 0.645814 | 8.1:+4564135 | MS.gene88674:CDS |
| TGGACGACTCACATGGTGCG+TGG | 0.646565 | 8.1:+4572706 | MS.gene88674:CDS |
| CTAAAGGAATGACATCATAC+GGG | 0.653617 | 8.1:-4572493 | None:intergenic |
| AGGATGTTTGTATGGAAATG+TGG | 0.655140 | 8.1:-4564235 | None:intergenic |
| TGCCATAGTGTGTAATATGT+GGG | 0.660167 | 8.1:-4572331 | None:intergenic |
| CAGGCCGGGGAATGATAGCA+GGG | 0.660857 | 8.1:+4564554 | MS.gene88674:CDS |
| TCAGAAATGATAATGAACAC+GGG | 0.662146 | 8.1:-4564613 | None:intergenic |
| AAGATGATCCAATAATGGAG+AGG | 0.665042 | 8.1:-4572773 | None:intergenic |
| GTTCTTCTCTCTCACAACTG+TGG | 0.667911 | 8.1:-4564339 | None:intergenic |
| TGCAGCCATGGATAGTGACG+AGG | 0.679797 | 8.1:+4562767 | None:intergenic |
| GTCCAAATGGACGACTCACA+TGG | 0.681562 | 8.1:+4572699 | MS.gene88674:CDS |
| TCATACGGGTTGTCTCCTTG+AGG | 0.685652 | 8.1:-4572479 | None:intergenic |
| ATTGAGTGGGGTAAAAGCCA+TGG | 0.686263 | 8.1:+4564367 | MS.gene88674:CDS |
| TCTCAGAGAAAGCTACACCT+GGG | 0.686701 | 8.1:+4573661 | MS.gene88674:intron |
| GACGACATGATGGACAGTAT+GGG | 0.690308 | 8.1:+4573226 | MS.gene88674:CDS |
| GAAGAACACCTGATTGAGTG+GGG | 0.693168 | 8.1:+4564355 | MS.gene88674:CDS |
| ACTGCACAAGTGTTTGGCTG+AGG | 0.693657 | 8.1:+4573781 | MS.gene88674:CDS |
| ATTCATCCAAGAAGGTCCTG+AGG | 0.699592 | 8.1:-4563798 | None:intergenic |
| TGTATTCAGATGGAAAACTG+AGG | 0.723155 | 8.1:+4571770 | MS.gene88674:CDS |
| AAGAATCATACAGTTAAACG+AGG | 0.733021 | 8.1:+4563850 | MS.gene88674:CDS |
| GGGGCTGTAAAACTCCACCG+AGG | 0.821008 | 8.1:-4564149 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 4562774 | 4573817 | 4562774 | ID=MS.gene88674 |
| chr8.1 | mRNA | 4562774 | 4573817 | 4562774 | ID=MS.gene88674.t1;Parent=MS.gene88674 |
| chr8.1 | exon | 4562774 | 4562845 | 4562774 | ID=MS.gene88674.t1.exon1;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4562774 | 4562845 | 4562774 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4563743 | 4563871 | 4563743 | ID=MS.gene88674.t1.exon2;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4563743 | 4563871 | 4563743 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4564121 | 4564415 | 4564121 | ID=MS.gene88674.t1.exon3;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4564121 | 4564415 | 4564121 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4564542 | 4564660 | 4564542 | ID=MS.gene88674.t1.exon4;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4564542 | 4564660 | 4564542 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4565238 | 4565370 | 4565238 | ID=MS.gene88674.t1.exon5;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4565238 | 4565370 | 4565238 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4567822 | 4567898 | 4567822 | ID=MS.gene88674.t1.exon6;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4567822 | 4567898 | 4567822 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4571634 | 4571831 | 4571634 | ID=MS.gene88674.t1.exon7;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4571634 | 4571831 | 4571634 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4572326 | 4572515 | 4572326 | ID=MS.gene88674.t1.exon8;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4572326 | 4572515 | 4572326 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4572656 | 4572834 | 4572656 | ID=MS.gene88674.t1.exon9;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4572656 | 4572834 | 4572656 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4573169 | 4573269 | 4573169 | ID=MS.gene88674.t1.exon10;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4573169 | 4573269 | 4573169 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| chr8.1 | exon | 4573667 | 4573817 | 4573667 | ID=MS.gene88674.t1.exon11;Parent=MS.gene88674.t1 |
| chr8.1 | CDS | 4573667 | 4573817 | 4573667 | ID=cds.MS.gene88674.t1;Parent=MS.gene88674.t1 |
| Gene Sequence |
| Protein sequence |
