Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88912.t1 | XP_003610296.2 | 86.9 | 153 | 17 | 2 | 1 | 150 | 38 | 190 | 1.90E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88912.t1 | G7JFL0 | 86.9 | 153 | 17 | 2 | 1 | 150 | 1 | 153 | 1.3e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene88912 | 0.804215 | 2.40E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88912.t1 | MTR_4g130640 | 86.928 | 153 | 17 | 2 | 1 | 150 | 1 | 153 | 5.53e-92 | 264 |
| MS.gene88912.t1 | MTR_7g069410 | 45.033 | 151 | 41 | 5 | 1 | 150 | 1 | 110 | 6.04e-28 | 100 |
| MS.gene88912.t1 | MTR_6g023775 | 41.237 | 97 | 23 | 2 | 80 | 142 | 65 | 161 | 2.00e-13 | 64.3 |
| MS.gene88912.t1 | MTR_7g031710 | 70.270 | 37 | 11 | 0 | 113 | 149 | 42 | 78 | 4.18e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTTGAAAAGAAATAAAA+TGG | 0.207796 | 1.1:+70827500 | MS.gene88912:CDS |
| GAAATAAAATGGATCGAAAA+AGG | 0.213477 | 1.1:+70827511 | MS.gene88912:CDS |
| CATCATGTTCTGAGAGAAAT+TGG | 0.219553 | 1.1:+70827457 | MS.gene88912:CDS |
| CTAGTAACACTTTCTTCTAT+TGG | 0.245408 | 1.1:-70827237 | None:intergenic |
| TAGTGGTTTACTATCATCTT+GGG | 0.287206 | 1.1:-70827280 | None:intergenic |
| TTTCACTTATTGAAAATTGC+TGG | 0.358067 | 1.1:+70827411 | MS.gene88912:CDS |
| AATTGCTGGTGAAGGAGTTC+AGG | 0.416502 | 1.1:+70827425 | MS.gene88912:CDS |
| TGAGTTATCTTTAGATGAAC+CGG | 0.418024 | 1.1:+70827587 | MS.gene88912:CDS |
| GCTACAATGTTCTTTGATGA+TGG | 0.418641 | 1.1:+70827618 | MS.gene88912:CDS |
| CTGCAGGTGGAAACAAGCTT+TGG | 0.435890 | 1.1:+70827337 | MS.gene88912:CDS |
| TAGAAGAAAGTGTTACTAGT+TGG | 0.440821 | 1.1:+70827241 | MS.gene88912:CDS |
| AACATTGTAGCCTCCAACTC+CGG | 0.456994 | 1.1:-70827606 | None:intergenic |
| ATAGTGGTTTACTATCATCT+TGG | 0.457884 | 1.1:-70827281 | None:intergenic |
| ATCTTTAGATGAACCGGAGT+TGG | 0.478188 | 1.1:+70827593 | MS.gene88912:CDS |
| GTAACACTTTCTTCTATTGG+AGG | 0.479958 | 1.1:-70827234 | None:intergenic |
| AAGATGATAGTAAACCACTA+TGG | 0.482539 | 1.1:+70827283 | MS.gene88912:CDS |
| TTTAGATGAACCGGAGTTGG+AGG | 0.500804 | 1.1:+70827596 | MS.gene88912:CDS |
| ATGTTCTTTGATGATGGAAA+CGG | 0.513119 | 1.1:+70827624 | MS.gene88912:CDS |
| GTGCATACTAATGTTGGAGT+TGG | 0.524300 | 1.1:+70827552 | MS.gene88912:CDS |
| GTTTATGTGCATACTAATGT+TGG | 0.532305 | 1.1:+70827546 | MS.gene88912:CDS |
| TCCTCTGAAGTAGTGAATGT+TGG | 0.537803 | 1.1:+70827675 | MS.gene88912:CDS |
| AGTGGTTTACTATCATCTTG+GGG | 0.567164 | 1.1:-70827279 | None:intergenic |
| AGAGGAAACACGGATTGTAT+CGG | 0.567785 | 1.1:-70827658 | None:intergenic |
| TTATTGAAAATTGCTGGTGA+AGG | 0.583272 | 1.1:+70827417 | MS.gene88912:CDS |
| TTCTTTGATGATGGAAACGG+AGG | 0.597380 | 1.1:+70827627 | MS.gene88912:CDS |
| GTGCAGAATAATGATTCTGC+AGG | 0.611185 | 1.1:+70827321 | MS.gene88912:CDS |
| CAGAATAATGATTCTGCAGG+TGG | 0.615421 | 1.1:+70827324 | MS.gene88912:CDS |
| TGATGATGGAAACGGAGGAG+AGG | 0.621097 | 1.1:+70827632 | MS.gene88912:CDS |
| TCCAACATTCACTACTTCAG+AGG | 0.626874 | 1.1:-70827676 | None:intergenic |
| TCACTACTTCAGAGGAAACA+CGG | 0.630269 | 1.1:-70827668 | None:intergenic |
| ACAGTGACAAATCTCCATAG+TGG | 0.653867 | 1.1:-70827297 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTTTGAAAAGAAATAAAA+TGG | + | chr1.1:70827500-70827519 | MS.gene88912:CDS | 15.0% |
| !! | AAATAAAATGGATCGAAAAA+GGG | + | chr1.1:70827512-70827531 | MS.gene88912:CDS | 20.0% |
| ! | GAAATAAAATGGATCGAAAA+AGG | + | chr1.1:70827511-70827530 | MS.gene88912:CDS | 25.0% |
| ! | TTTCACTTATTGAAAATTGC+TGG | + | chr1.1:70827411-70827430 | MS.gene88912:CDS | 25.0% |
| AAGATGATAGTAAACCACTA+TGG | + | chr1.1:70827283-70827302 | MS.gene88912:CDS | 30.0% | |
| ATAGTGGTTTACTATCATCT+TGG | - | chr1.1:70827284-70827303 | None:intergenic | 30.0% | |
| CTAGTAACACTTTCTTCTAT+TGG | - | chr1.1:70827240-70827259 | None:intergenic | 30.0% | |
| GTTTATGTGCATACTAATGT+TGG | + | chr1.1:70827546-70827565 | MS.gene88912:CDS | 30.0% | |
| TAGTGGTTTACTATCATCTT+GGG | - | chr1.1:70827283-70827302 | None:intergenic | 30.0% | |
| TGAGTTATCTTTAGATGAAC+CGG | + | chr1.1:70827587-70827606 | MS.gene88912:CDS | 30.0% | |
| TTATTGAAAATTGCTGGTGA+AGG | + | chr1.1:70827417-70827436 | MS.gene88912:CDS | 30.0% | |
| !! | ATGTTCTTTGATGATGGAAA+CGG | + | chr1.1:70827624-70827643 | MS.gene88912:CDS | 30.0% |
| !! | TAGAAGAAAGTGTTACTAGT+TGG | + | chr1.1:70827241-70827260 | MS.gene88912:CDS | 30.0% |
| AGTGGTTTACTATCATCTTG+GGG | - | chr1.1:70827282-70827301 | None:intergenic | 35.0% | |
| GCTACAATGTTCTTTGATGA+TGG | + | chr1.1:70827618-70827637 | MS.gene88912:CDS | 35.0% | |
| GTAACACTTTCTTCTATTGG+AGG | - | chr1.1:70827237-70827256 | None:intergenic | 35.0% | |
| ! | ACTTTTGTCAAAAAGAGGTG+AGG | + | chr1.1:70827367-70827386 | MS.gene88912:CDS | 35.0% |
| ! | GTGCAACTTTTGTCAAAAAG+AGG | + | chr1.1:70827362-70827381 | MS.gene88912:CDS | 35.0% |
| !! | CATCATGTTCTGAGAGAAAT+TGG | + | chr1.1:70827457-70827476 | MS.gene88912:CDS | 35.0% |
| ACAGTGACAAATCTCCATAG+TGG | - | chr1.1:70827300-70827319 | None:intergenic | 40.0% | |
| ATCTTTAGATGAACCGGAGT+TGG | + | chr1.1:70827593-70827612 | MS.gene88912:CDS | 40.0% | |
| CAGAATAATGATTCTGCAGG+TGG | + | chr1.1:70827324-70827343 | MS.gene88912:CDS | 40.0% | |
| GTGCAGAATAATGATTCTGC+AGG | + | chr1.1:70827321-70827340 | MS.gene88912:CDS | 40.0% | |
| GTGCATACTAATGTTGGAGT+TGG | + | chr1.1:70827552-70827571 | MS.gene88912:CDS | 40.0% | |
| TCACTACTTCAGAGGAAACA+CGG | - | chr1.1:70827671-70827690 | None:intergenic | 40.0% | |
| TCCAACATTCACTACTTCAG+AGG | - | chr1.1:70827679-70827698 | None:intergenic | 40.0% | |
| TCGAAAAAGGGCTGAAGATT+TGG | + | chr1.1:70827524-70827543 | MS.gene88912:CDS | 40.0% | |
| ! | AGAGGAAACACGGATTGTAT+CGG | - | chr1.1:70827661-70827680 | None:intergenic | 40.0% |
| !! | TCCTCTGAAGTAGTGAATGT+TGG | + | chr1.1:70827675-70827694 | MS.gene88912:CDS | 40.0% |
| !! | TTCTTTGATGATGGAAACGG+AGG | + | chr1.1:70827627-70827646 | MS.gene88912:CDS | 40.0% |
| AACATTGTAGCCTCCAACTC+CGG | - | chr1.1:70827609-70827628 | None:intergenic | 45.0% | |
| AATTGCTGGTGAAGGAGTTC+AGG | + | chr1.1:70827425-70827444 | MS.gene88912:CDS | 45.0% | |
| TTTAGATGAACCGGAGTTGG+AGG | + | chr1.1:70827596-70827615 | MS.gene88912:CDS | 45.0% | |
| CTGCAGGTGGAAACAAGCTT+TGG | + | chr1.1:70827337-70827356 | MS.gene88912:CDS | 50.0% | |
| !! | TGATGATGGAAACGGAGGAG+AGG | + | chr1.1:70827632-70827651 | MS.gene88912:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70827231 | 70827704 | 70827231 | ID=MS.gene88912 |
| chr1.1 | mRNA | 70827231 | 70827704 | 70827231 | ID=MS.gene88912.t1;Parent=MS.gene88912 |
| chr1.1 | exon | 70827231 | 70827704 | 70827231 | ID=MS.gene88912.t1.exon1;Parent=MS.gene88912.t1 |
| chr1.1 | CDS | 70827231 | 70827704 | 70827231 | ID=cds.MS.gene88912.t1;Parent=MS.gene88912.t1 |
| Gene Sequence |
| Protein sequence |
