Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88916.t1 | XP_003592080.1 | 95.1 | 184 | 9 | 0 | 1 | 184 | 1 | 184 | 2.00E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88916.t1 | G7IBX7 | 95.1 | 184 | 9 | 0 | 1 | 184 | 1 | 184 | 1.5e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056403 | MS.gene88916 | 0.81508 | 1.11E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene88916 | 0.82378 | 1.15E-53 | -1.69E-46 |
| MS.gene060586 | MS.gene88916 | 0.808829 | 2.55E-50 | -1.69E-46 |
| MS.gene060830 | MS.gene88916 | 0.829531 | 4.89E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88916.t1 | MTR_1g098510 | 95.109 | 184 | 9 | 0 | 1 | 184 | 1 | 184 | 4.89e-129 | 360 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88916.t1 | AT5G09270 | 56.842 | 95 | 36 | 2 | 90 | 184 | 19 | 108 | 1.65e-31 | 110 |
| MS.gene88916.t1 | AT5G09270 | 56.842 | 95 | 36 | 2 | 90 | 184 | 19 | 108 | 1.65e-31 | 110 |
Find 52 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCCTGCTGCAGGCTTTA+TGG | 0.092653 | 1.1:+70865982 | MS.gene88916:CDS |
| CTCTTCTGCTACGGGCTTTA+TGG | 0.095185 | 1.1:+70865811 | MS.gene88916:CDS |
| TATATGGCGGATTTGGTCTT+TGG | 0.234560 | 1.1:+70866083 | MS.gene88916:CDS |
| ATTCTTGTATATGGCGGATT+TGG | 0.303440 | 1.1:+70866076 | MS.gene88916:CDS |
| ATAGATATAGAAAGCTTCTT+AGG | 0.309898 | 1.1:+70866113 | MS.gene88916:CDS |
| GGTTCCTATATTCTTGTATA+TGG | 0.337081 | 1.1:+70866067 | MS.gene88916:CDS |
| TTCTGCCAAAGTGGTTGAAA+AGG | 0.353945 | 1.1:+70866222 | MS.gene88916:CDS |
| CTTGGTCCTCACCAGTTTCC+AGG | 0.356142 | 1.1:-70865949 | None:intergenic |
| TGCAGGCTTTATGGATTCAT+TGG | 0.369416 | 1.1:+70865991 | MS.gene88916:CDS |
| CAGAAACTTATCCTTATGTA+TGG | 0.372733 | 1.1:+70866044 | MS.gene88916:CDS |
| TGGATAAGTGCTTCAGAACT+TGG | 0.383586 | 1.1:-70865758 | None:intergenic |
| TTATGGATTCATTGGAAAAT+CGG | 0.384322 | 1.1:+70865999 | MS.gene88916:CDS |
| TGATTTGCCATGTCCTTGGC+TGG | 0.408407 | 1.1:-70865881 | None:intergenic |
| AAGAAGCTTTCTATATCTAT+AGG | 0.413891 | 1.1:-70866110 | None:intergenic |
| GTCGGTGCCCCTCCTGCTGC+AGG | 0.419080 | 1.1:+70865974 | MS.gene88916:CDS |
| CAGGAGGGGCACCGACTACT+TGG | 0.421600 | 1.1:-70865967 | None:intergenic |
| AAGTTCTGCAGCACCTATCC+TGG | 0.422828 | 1.1:+70865931 | MS.gene88916:CDS |
| AGGATAGGTGCTGCAGAACT+TGG | 0.446859 | 1.1:-70865929 | None:intergenic |
| AGTCGGTGGCTCTTCTGCTA+CGG | 0.454743 | 1.1:+70865802 | MS.gene88916:CDS |
| GCAGCACCTATCCTGGAAAC+TGG | 0.465240 | 1.1:+70865938 | MS.gene88916:CDS |
| GAAGCACTTATCCAGAAAAC+TGG | 0.468163 | 1.1:+70865767 | MS.gene88916:CDS |
| GTCGGTGGCTCTTCTGCTAC+GGG | 0.474768 | 1.1:+70865803 | MS.gene88916:CDS |
| TCCGCCATATACAAGAATAT+AGG | 0.486205 | 1.1:-70866071 | None:intergenic |
| TGGTTGATTTGCCATGTCCT+TGG | 0.486730 | 1.1:-70865885 | None:intergenic |
| ACTACGTGAGATGGTTGAAA+AGG | 0.493284 | 1.1:+70866168 | MS.gene88916:CDS |
| AGCTTCTTAGGACAGAGGAC+AGG | 0.494425 | 1.1:+70866125 | MS.gene88916:CDS |
| AGAAACTTATCCTTATGTAT+GGG | 0.501079 | 1.1:+70866045 | MS.gene88916:CDS |
| TCTGCCAAAGTGGTTGAAAA+GGG | 0.502981 | 1.1:+70866223 | MS.gene88916:CDS |
| ATATAGGAACCCCATACATA+AGG | 0.508413 | 1.1:-70866055 | None:intergenic |
| TCCTATATTCTTGTATATGG+CGG | 0.519420 | 1.1:+70866070 | MS.gene88916:CDS |
| TTCTAGTAATTCTGCCAAAG+TGG | 0.522258 | 1.1:+70866213 | MS.gene88916:CDS |
| ATGTTGCAATGTAGGCTACT+TGG | 0.523428 | 1.1:-70866269 | None:intergenic |
| CATAAAGCCTGCAGCAGGAG+GGG | 0.525453 | 1.1:-70865981 | None:intergenic |
| TCCTCACCAGTTTCCAGGAT+AGG | 0.526596 | 1.1:-70865944 | None:intergenic |
| TCCATAAAGCCTGCAGCAGG+AGG | 0.530529 | 1.1:-70865983 | None:intergenic |
| TTAGGACAGAGGACAGGGTA+CGG | 0.537011 | 1.1:+70866131 | MS.gene88916:CDS |
| GAATCCATAAAGCCTGCAGC+AGG | 0.543830 | 1.1:-70865986 | None:intergenic |
| TAGGACAGAGGACAGGGTAC+GGG | 0.558112 | 1.1:+70866132 | MS.gene88916:CDS |
| GGAAGGAATCTCAGAAGATG+TGG | 0.565631 | 1.1:-70866248 | None:intergenic |
| GAAACTTATCCTTATGTATG+GGG | 0.570070 | 1.1:+70866046 | MS.gene88916:CDS |
| TCAAGCAAGACTACGTGAGA+TGG | 0.592566 | 1.1:+70866159 | MS.gene88916:CDS |
| CCATAAAGCCTGCAGCAGGA+GGG | 0.618716 | 1.1:-70865982 | None:intergenic |
| TAGCTGGAACACTCCAGCCA+AGG | 0.621030 | 1.1:+70865868 | MS.gene88916:CDS |
| GAACACTCCAGCCAAGGACA+TGG | 0.636957 | 1.1:+70865874 | MS.gene88916:CDS |
| GCTTCTTAGGACAGAGGACA+GGG | 0.639564 | 1.1:+70866126 | MS.gene88916:CDS |
| ACCTATCCTGGAAACTGGTG+AGG | 0.641675 | 1.1:+70865943 | MS.gene88916:CDS |
| TGCAATGTAGGCTACTTGGA+AGG | 0.646398 | 1.1:-70866265 | None:intergenic |
| ACTGGCGAGGATCAAGTAGT+CGG | 0.654403 | 1.1:+70865785 | MS.gene88916:CDS |
| TAGAAAGCTTCTTAGGACAG+AGG | 0.663471 | 1.1:+70866120 | MS.gene88916:CDS |
| ACTGGTGAGGACCAAGTAGT+CGG | 0.664340 | 1.1:+70865956 | MS.gene88916:CDS |
| GGCGAGGATCAAGTAGTCGG+TGG | 0.665719 | 1.1:+70865788 | MS.gene88916:CDS |
| ACTTATCCAGAAAACTGGCG+AGG | 0.696112 | 1.1:+70865772 | MS.gene88916:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGGATTCATTGAAAAAT+CGG | + | chr1.1:70865828-70865847 | MS.gene88916:CDS | 20.0% |
| ! | AAGAAGCTTTCTATATCTAT+AGG | - | chr1.1:70866113-70866132 | None:intergenic | 25.0% |
| ! | AGAAACTTATCCTTATGTAT+GGG | + | chr1.1:70866045-70866064 | MS.gene88916:CDS | 25.0% |
| ! | ATAGATATAGAAAGCTTCTT+AGG | + | chr1.1:70866113-70866132 | MS.gene88916:CDS | 25.0% |
| ! | ATCAAAAAATCATAGATAGC+TGG | + | chr1.1:70865852-70865871 | MS.gene88916:CDS | 25.0% |
| ! | TTATGGATTCATTGGAAAAT+CGG | + | chr1.1:70865999-70866018 | MS.gene88916:CDS | 25.0% |
| CAGAAACTTATCCTTATGTA+TGG | + | chr1.1:70866044-70866063 | MS.gene88916:CDS | 30.0% | |
| GAAACTTATCCTTATGTATG+GGG | + | chr1.1:70866046-70866065 | MS.gene88916:CDS | 30.0% | |
| GGTTCCTATATTCTTGTATA+TGG | + | chr1.1:70866067-70866086 | MS.gene88916:CDS | 30.0% | |
| TCCTATATTCTTGTATATGG+CGG | + | chr1.1:70866070-70866089 | MS.gene88916:CDS | 30.0% | |
| !!! | GCTTGTTGCTTTTCATTATT+TGG | - | chr1.1:70865908-70865927 | None:intergenic | 30.0% |
| ATATAGGAACCCCATACATA+AGG | - | chr1.1:70866058-70866077 | None:intergenic | 35.0% | |
| TCCGCCATATACAAGAATAT+AGG | - | chr1.1:70866074-70866093 | None:intergenic | 35.0% | |
| TTCTAGTAATTCTGCCAAAG+TGG | + | chr1.1:70866213-70866232 | MS.gene88916:CDS | 35.0% | |
| !! | ATTCTTGTATATGGCGGATT+TGG | + | chr1.1:70866076-70866095 | MS.gene88916:CDS | 35.0% |
| !!! | GCTTGCTGCTTTTCATTATT+TGG | - | chr1.1:70865737-70865756 | None:intergenic | 35.0% |
| ACTACGTGAGATGGTTGAAA+AGG | + | chr1.1:70866168-70866187 | MS.gene88916:CDS | 40.0% | |
| TAGAAAGCTTCTTAGGACAG+AGG | + | chr1.1:70866120-70866139 | MS.gene88916:CDS | 40.0% | |
| TCTGCCAAAGTGGTTGAAAA+GGG | + | chr1.1:70866223-70866242 | MS.gene88916:CDS | 40.0% | |
| TGGATAAGTGCTTCAGAACT+TGG | - | chr1.1:70865761-70865780 | None:intergenic | 40.0% | |
| TTCTGCCAAAGTGGTTGAAA+AGG | + | chr1.1:70866222-70866241 | MS.gene88916:CDS | 40.0% | |
| ! | GAAGCACTTATCCAGAAAAC+TGG | + | chr1.1:70865767-70865786 | MS.gene88916:CDS | 40.0% |
| ! | TGCAGGCTTTATGGATTCAT+TGG | + | chr1.1:70865991-70866010 | MS.gene88916:CDS | 40.0% |
| !! | TATATGGCGGATTTGGTCTT+TGG | + | chr1.1:70866083-70866102 | MS.gene88916:CDS | 40.0% |
| ACTTATCCAGAAAACTGGCG+AGG | + | chr1.1:70865772-70865791 | MS.gene88916:CDS | 45.0% | |
| GGAAGGAATCTCAGAAGATG+TGG | - | chr1.1:70866251-70866270 | None:intergenic | 45.0% | |
| TCAAGCAAGACTACGTGAGA+TGG | + | chr1.1:70866159-70866178 | MS.gene88916:CDS | 45.0% | |
| ! | GGAACCCTTTTCAACCACTT+TGG | - | chr1.1:70866230-70866249 | None:intergenic | 45.0% |
| !! | TGGTTGATTTGCCATGTCCT+TGG | - | chr1.1:70865888-70865907 | None:intergenic | 45.0% |
| AAGTTCTGCAGCACCTATCC+TGG | + | chr1.1:70865931-70865950 | MS.gene88916:CDS | 50.0% | |
| ACCTATCCTGGAAACTGGTG+AGG | + | chr1.1:70865943-70865962 | MS.gene88916:CDS | 50.0% | |
| ACTGGCGAGGATCAAGTAGT+CGG | + | chr1.1:70865785-70865804 | MS.gene88916:CDS | 50.0% | |
| ACTGGTGAGGACCAAGTAGT+CGG | + | chr1.1:70865956-70865975 | MS.gene88916:CDS | 50.0% | |
| AGCTTCTTAGGACAGAGGAC+AGG | + | chr1.1:70866125-70866144 | MS.gene88916:CDS | 50.0% | |
| CTCTTCTGCTACGGGCTTTA+TGG | + | chr1.1:70865811-70865830 | MS.gene88916:CDS | 50.0% | |
| GAATCCATAAAGCCTGCAGC+AGG | - | chr1.1:70865989-70866008 | None:intergenic | 50.0% | |
| GCTTCTTAGGACAGAGGACA+GGG | + | chr1.1:70866126-70866145 | MS.gene88916:CDS | 50.0% | |
| TCCTCACCAGTTTCCAGGAT+AGG | - | chr1.1:70865947-70865966 | None:intergenic | 50.0% | |
| TTAGGACAGAGGACAGGGTA+CGG | + | chr1.1:70866131-70866150 | MS.gene88916:CDS | 50.0% | |
| ! | AGGATAGGTGCTGCAGAACT+TGG | - | chr1.1:70865932-70865951 | None:intergenic | 50.0% |
| ! | CTTGATCCTCGCCAGTTTTC+TGG | - | chr1.1:70865781-70865800 | None:intergenic | 50.0% |
| ! | TGATTTGCCATGTCCTTGGC+TGG | - | chr1.1:70865884-70865903 | None:intergenic | 50.0% |
| AGTCGGTGGCTCTTCTGCTA+CGG | + | chr1.1:70865802-70865821 | MS.gene88916:CDS | 55.0% | |
| CATAAAGCCTGCAGCAGGAG+GGG | - | chr1.1:70865984-70866003 | None:intergenic | 55.0% | |
| CCATAAAGCCTGCAGCAGGA+GGG | - | chr1.1:70865985-70866004 | None:intergenic | 55.0% | |
| CTTGGTCCTCACCAGTTTCC+AGG | - | chr1.1:70865952-70865971 | None:intergenic | 55.0% | |
| GAACACTCCAGCCAAGGACA+TGG | + | chr1.1:70865874-70865893 | MS.gene88916:CDS | 55.0% | |
| GCAGCACCTATCCTGGAAAC+TGG | + | chr1.1:70865938-70865957 | MS.gene88916:CDS | 55.0% | |
| TAGCTGGAACACTCCAGCCA+AGG | + | chr1.1:70865868-70865887 | MS.gene88916:CDS | 55.0% | |
| TAGGACAGAGGACAGGGTAC+GGG | + | chr1.1:70866132-70866151 | MS.gene88916:CDS | 55.0% | |
| TCCATAAAGCCTGCAGCAGG+AGG | - | chr1.1:70865986-70866005 | None:intergenic | 55.0% | |
| CCCTCCTGCTGCAGGCTTTA+TGG | + | chr1.1:70865982-70866001 | MS.gene88916:CDS | 60.0% | |
| GGCGAGGATCAAGTAGTCGG+TGG | + | chr1.1:70865788-70865807 | MS.gene88916:CDS | 60.0% | |
| GTCGGTGGCTCTTCTGCTAC+GGG | + | chr1.1:70865803-70865822 | MS.gene88916:CDS | 60.0% | |
| !! | CAGGAGGGGCACCGACTACT+TGG | - | chr1.1:70865970-70865989 | None:intergenic | 65.0% |
| ! | GTCGGTGCCCCTCCTGCTGC+AGG | + | chr1.1:70865974-70865993 | MS.gene88916:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70865722 | 70866276 | 70865722 | ID=MS.gene88916 |
| chr1.1 | mRNA | 70865722 | 70866276 | 70865722 | ID=MS.gene88916.t1;Parent=MS.gene88916 |
| chr1.1 | exon | 70865722 | 70866276 | 70865722 | ID=MS.gene88916.t1.exon1;Parent=MS.gene88916.t1 |
| chr1.1 | CDS | 70865722 | 70866276 | 70865722 | ID=cds.MS.gene88916.t1;Parent=MS.gene88916.t1 |
| Gene Sequence |
| Protein sequence |
