AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89028

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89028.t1 MTR_3g101010 72.093 387 107 1 29 415 3 388 0.0 554
MS.gene89028.t1 MTR_8g027395 68.844 398 119 2 17 414 3 395 0.0 553
MS.gene89028.t1 MTR_3g018740 71.465 389 107 2 29 416 3 388 0.0 546
MS.gene89028.t1 MTR_4g045707 70.466 386 111 1 30 415 5 387 0.0 542
MS.gene89028.t1 MTR_3g101030 66.169 402 115 5 32 415 30 428 0.0 518
MS.gene89028.t1 MTR_3g048000 64.649 413 122 5 7 416 1 392 0.0 509
MS.gene89028.t1 MTR_8g027420 66.927 384 111 2 31 414 6 373 6.71e-179 503
MS.gene89028.t1 MTR_2g045980 65.816 392 124 4 29 416 3 388 2.83e-174 492
MS.gene89028.t1 MTR_2g046030 65.306 392 126 4 29 416 3 388 3.30e-174 492
MS.gene89028.t1 MTR_4g088525 68.579 366 110 2 50 415 8 368 6.67e-173 488
MS.gene89028.t1 MTR_8g461160 64.031 392 128 3 31 414 6 392 2.99e-172 487
MS.gene89028.t1 MTR_7g050810 62.404 391 144 2 27 414 12 402 1.85e-170 483
MS.gene89028.t1 MTR_7g451890 61.893 391 146 2 27 414 10 400 3.62e-170 482
MS.gene89028.t1 MTR_7g047780 60.870 391 150 2 27 414 10 400 2.11e-167 475
MS.gene89028.t1 MTR_3g101180 61.654 399 142 4 26 414 1 398 8.98e-167 474
MS.gene89028.t1 MTR_7g050750 61.893 391 146 2 27 414 4 394 1.14e-166 473
MS.gene89028.t1 MTR_4g088535 64.829 381 113 2 31 411 7 366 2.74e-166 472
MS.gene89028.t1 MTR_3g101130 60.759 395 149 2 26 418 1 391 3.68e-166 472
MS.gene89028.t1 MTR_3g101050 64.767 386 114 6 33 415 31 397 2.99e-162 462
MS.gene89028.t1 MTR_3g015620 58.435 409 135 5 9 414 2 378 2.31e-153 439
MS.gene89028.t1 MTR_3g102920 56.174 413 128 8 6 414 45 408 9.14e-140 405
MS.gene89028.t1 MTR_3g101200 52.427 412 161 6 7 414 10 390 1.22e-128 376
MS.gene89028.t1 MTR_3g100995 71.094 256 70 2 159 414 11 262 3.22e-128 370
MS.gene89028.t1 MTR_3g101190 54.121 364 99 3 53 414 13 310 3.97e-127 370
MS.gene89028.t1 MTR_3g100010 55.556 351 107 5 15 332 2 336 2.31e-118 348
MS.gene89028.t1 MTR_3g018747 69.202 263 64 3 155 416 9 255 1.73e-116 340
MS.gene89028.t1 MTR_7g050830 54.711 329 110 4 16 322 7 318 5.45e-113 334
MS.gene89028.t1 MTR_3g015760 59.058 276 108 3 140 414 8 279 3.20e-102 305
MS.gene89028.t1 MTR_3g114310 41.795 390 203 12 39 413 27 407 2.36e-84 264
MS.gene89028.t1 MTR_3g099970 65.174 201 53 2 150 350 15 198 7.41e-84 255
MS.gene89028.t1 MTR_3g100520 59.910 222 73 3 196 415 1 208 9.68e-83 253
MS.gene89028.t1 MTR_2g046410 67.308 208 57 4 215 416 147 349 3.84e-82 256
MS.gene89028.t1 MTR_3g111160 63.551 214 74 3 203 415 9 219 4.26e-82 251
MS.gene89028.t1 MTR_3g101110 57.322 239 73 3 155 390 68 280 5.69e-79 246
MS.gene89028.t1 MTR_3g111150 62.921 178 42 2 34 211 2 155 5.79e-70 218
MS.gene89028.t1 MTR_3g101020 57.527 186 51 2 229 414 1 158 5.74e-64 202
MS.gene89028.t1 MTR_3g101150 63.291 158 55 1 261 415 1 158 3.42e-61 196
MS.gene89028.t1 MTR_3g102870 65.772 149 51 0 220 368 3 151 5.76e-58 187
MS.gene89028.t1 MTR_3g100990 59.603 151 60 1 30 180 4 153 2.05e-49 175
MS.gene89028.t1 MTR_4g093550 68.605 86 27 0 29 114 4 89 4.79e-34 122
MS.gene89028.t1 MTR_6g046890 60.465 86 29 1 39 119 7 92 1.44e-23 95.9
MS.gene89028.t1 MTR_3g101140 56.818 88 32 2 331 415 31 115 2.52e-20 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89028.t1 AT1G47710 55.013 389 171 4 29 414 3 390 1.04e-150 432
MS.gene89028.t1 AT1G47710 55.013 389 171 4 29 414 30 417 5.53e-150 432
MS.gene89028.t1 AT3G45220 48.855 393 184 5 31 413 5 390 4.22e-129 378
MS.gene89028.t1 AT2G25240 48.980 392 177 9 31 413 5 382 4.01e-121 357
MS.gene89028.t1 AT2G26390 47.222 396 182 9 31 413 5 386 4.31e-114 339
MS.gene89028.t1 AT1G62170 43.360 369 179 4 53 415 89 433 9.92e-104 314
MS.gene89028.t1 AT2G14540 43.188 389 195 5 29 413 31 397 2.62e-98 300
MS.gene89028.t1 AT1G64030 40.256 390 207 5 29 413 3 371 1.56e-96 294
MS.gene89028.t1 AT1G62170 39.801 402 179 5 53 415 89 466 1.66e-96 297
MS.gene89028.t1 AT2G35580 42.818 369 180 8 54 413 25 371 2.00e-92 283
MS.gene89028.t1 AT1G64010 45.205 219 95 4 196 413 1 195 4.46e-59 191
MS.gene89028.t1 AT1G64010 44.608 204 88 4 211 413 2 181 1.54e-51 171
MS.gene89028.t1 AT1G62160 42.703 185 80 3 233 415 60 220 2.04e-34 127
MS.gene89028.t1 AT1G63280 52.542 118 52 2 152 267 5 120 3.70e-33 121
MS.gene89028.t1 AT1G51330 55.319 94 42 0 153 246 29 122 2.66e-25 102
MS.gene89028.t1 AT1G64020 35.433 127 71 5 23 145 1 120 3.41e-15 72.0

Find 86 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATGCACTGTACTTTAA+AGG 0.104069 2.4:+1912078 MS.gene89028:CDS
TGACTAAAGAAGTGAATTTA+TGG 0.193244 2.4:+1911977 MS.gene89028:CDS
ATCTCAACTCCTTTGCTTCT+CGG 0.254417 2.4:+1910790 MS.gene89028:CDS
TGAGCACTTGTCCCATAAAA+AGG 0.260936 2.4:-1912713 None:intergenic
AATCGTGTCTTCTAATTATA+AGG 0.276657 2.4:+1910923 MS.gene89028:CDS
GATGATCAATGGATTCAAAT+TGG 0.306689 2.4:-1910770 None:intergenic
ATTTAGAAGGTGAAAATCAT+AGG 0.313217 2.4:-1912139 None:intergenic
AATCTTCTCTAATTAGAAAT+AGG 0.325707 2.4:-1912677 None:intergenic
CCAAGATTCGAAGTTTCATT+TGG 0.330742 2.4:+1912408 MS.gene89028:CDS
CCAAATGAAACTTCGAATCT+TGG 0.339486 2.4:-1912408 None:intergenic
GTTCTCAGCATCATGGTTGC+AGG 0.345670 2.4:+1910708 MS.gene89028:CDS
AATACGTTGCTTCTCCTTGC+TGG 0.356948 2.4:-1912190 None:intergenic
TCTCTTTCGTCAATGGTGTA+TGG 0.361510 2.4:+1910862 MS.gene89028:CDS
CCTCGCCTCTCTTTCGTCAA+TGG 0.369673 2.4:+1910855 MS.gene89028:CDS
TGCTGTAACTAAAAGACGTC+TGG 0.371226 2.4:+1912602 MS.gene89028:CDS
TGACCGAGTTACCATTTAGA+AGG 0.390936 2.4:-1912152 None:intergenic
ATTCACCTACTTCGACTTGT+TGG 0.406289 2.4:-1912377 None:intergenic
TTAAAGAAATTCTTCCTCAA+TGG 0.410577 2.4:+1912025 MS.gene89028:CDS
GAAAACACTATGTTGTTGTT+AGG 0.411549 2.4:-1910666 None:intergenic
AGAAGACACGATTTGTTTGA+AGG 0.417491 2.4:-1910913 None:intergenic
TGAAACTTCTGATATGTTGA+AGG 0.423567 2.4:+1912434 MS.gene89028:CDS
CTTCTCCTTGCTGGTCATGA+AGG 0.423720 2.4:-1912181 None:intergenic
TTCACCTACTTCGACTTGTT+GGG 0.430525 2.4:-1912376 None:intergenic
TGAAGGAAGGTTGAATAGAA+AGG 0.434496 2.4:-1910896 None:intergenic
AGTGAATTTATGGGCTGAAA+AGG 0.440578 2.4:+1911987 MS.gene89028:CDS
CTGCAGGAGCAGCATCTTTG+AGG 0.452846 2.4:-1910827 None:intergenic
GACTAAAGAAGTGAATTTAT+GGG 0.454605 2.4:+1911978 MS.gene89028:CDS
AGCAGCATCTTTGAGGATGA+TGG 0.454926 2.4:-1910820 None:intergenic
CTCAAAGATGCTGCTCCTGC+AGG 0.456434 2.4:+1910828 MS.gene89028:CDS
GTCAGACCTCCATGTGAGAA+AGG 0.458548 2.4:-1912474 None:intergenic
AAAATGTGGTTTGCCAGCTT+TGG 0.471823 2.4:+1912305 MS.gene89028:CDS
ATCATGGTTGCAGGCTCTGA+GGG 0.473422 2.4:+1910717 MS.gene89028:CDS
CTAATTAGAGAAGATTTGAC+TGG 0.480376 2.4:+1912684 MS.gene89028:CDS
CTCTTTCGTCAATGGTGTAT+GGG 0.485072 2.4:+1910863 MS.gene89028:CDS
CACTGTACTTTAAAGGAACA+TGG 0.491924 2.4:+1912085 MS.gene89028:CDS
TTCTCCTTGCTGGTCATGAA+GGG 0.493434 2.4:-1912180 None:intergenic
GAAGGAAGGTTGAATAGAAA+GGG 0.495431 2.4:-1910895 None:intergenic
GTCTTTCATTGAAGTAAATG+AGG 0.499860 2.4:+1912554 MS.gene89028:CDS
ACAACAAGGTCTTGATGTGC+AGG 0.500265 2.4:-1912513 None:intergenic
GCTACGTTCTTAACCAAAGC+TGG 0.501238 2.4:-1912318 None:intergenic
TTTAGAAGGTGAAAATCATA+GGG 0.502662 2.4:-1912138 None:intergenic
GAGAGGCGAGGTCCACCTGC+AGG 0.503900 2.4:-1910843 None:intergenic
GCTACAAAGTCTAGTCTAGT+TGG 0.503965 2.4:-1912642 None:intergenic
CTCTGTCTTCCTTATGAGCA+AGG 0.512387 2.4:+1912237 MS.gene89028:CDS
TACTGAGGGAAACATTTGCT+TGG 0.515683 2.4:-1910617 None:intergenic
CATCATGGTTGCAGGCTCTG+AGG 0.516370 2.4:+1910716 MS.gene89028:CDS
TCAAGTTGTTCTCAGCATCA+TGG 0.517438 2.4:+1910701 MS.gene89028:CDS
TTCTAATTATAAGGCCACTC+TGG 0.518433 2.4:+1910932 MS.gene89028:CDS
GACACGATTTGTTTGAAGGA+AGG 0.523677 2.4:-1910909 None:intergenic
TGCATTTGCAAAGATGAGCC+TGG 0.531197 2.4:-1912064 None:intergenic
AGTTAGGATCTTTCAGCAAG+AGG 0.536413 2.4:-1912738 None:intergenic
TGGGCTGAAAAGGAGACAAA+TGG 0.539906 2.4:+1911997 MS.gene89028:CDS
TTCATTGAAGTAAATGAGGA+AGG 0.545592 2.4:+1912558 MS.gene89028:CDS
AAAGGAGTTGAGATGATCAA+TGG 0.549631 2.4:-1910781 None:intergenic
CCTTCCCAACAAGTCGAAGT+AGG 0.559625 2.4:+1912372 MS.gene89028:CDS
GGAAACGAGCCGAGAAGCAA+AGG 0.561911 2.4:-1910799 None:intergenic
TAGAAATAGGAAAGGATGGT+CGG 0.570616 2.4:-1912664 None:intergenic
AAGCTTGTCTTGTAGTAACT+CGG 0.572074 2.4:-1912349 None:intergenic
GCTGTAACTAAAAGACGTCT+GGG 0.574288 2.4:+1912603 MS.gene89028:CDS
TCTGATATGTTGAAGGAGCT+AGG 0.574906 2.4:+1912441 MS.gene89028:CDS
TTCCGTTGATTTCGTAACCA+AGG 0.574990 2.4:+1910956 MS.gene89028:CDS
TTTGCTGCTTGATTCACAGT+GGG 0.582781 2.4:-1910538 None:intergenic
AAAGATGCTGCTCCTGCAGG+TGG 0.583593 2.4:+1910831 MS.gene89028:CDS
GTCGAGAAGCTGTTGTTGTG+TGG 0.586119 2.4:-1910742 None:intergenic
TCTAAATGGTAACTCGGTCA+AGG 0.590593 2.4:+1912155 MS.gene89028:CDS
TCACCTTCTAAATGGTAACT+CGG 0.590836 2.4:+1912149 MS.gene89028:CDS
AGAAATTCTTCCTCAATGGT+CGG 0.595842 2.4:+1912029 MS.gene89028:CDS
TAATTAGAAATAGGAAAGGA+TGG 0.603116 2.4:-1912668 None:intergenic
GATCAATGGATTCAAATTGG+AGG 0.604312 2.4:-1910767 None:intergenic
TCGAGAAGCTGTTGTTGTGT+GGG 0.605173 2.4:-1910741 None:intergenic
GTAAGATTACCTTTCTCACA+TGG 0.615349 2.4:+1912465 MS.gene89028:CDS
AGAACAACTTGAAGCGACAG+TGG 0.623083 2.4:-1910690 None:intergenic
TCTCTAATTAGAAATAGGAA+AGG 0.624979 2.4:-1912672 None:intergenic
TTACGAGGGAAACATCCTCA+AGG 0.632675 2.4:-1910578 None:intergenic
CCTACTTCGACTTGTTGGGA+AGG 0.636809 2.4:-1912372 None:intergenic
TACCTTGGTTACGAAATCAA+CGG 0.638063 2.4:-1910958 None:intergenic
GGTCGGTCGACAACTTAACC+AGG 0.646139 2.4:+1912046 MS.gene89028:CDS
ACAGATGTTTAGCGATACTG+AGG 0.651654 2.4:-1910632 None:intergenic
ATACACCATTGACGAAAGAG+AGG 0.661994 2.4:-1910860 None:intergenic
GAAATCAACGGAAGCCAGAG+TGG 0.666740 2.4:-1910946 None:intergenic
GGTTCCCTTCATGACCAGCA+AGG 0.674176 2.4:+1912176 MS.gene89028:CDS
AGATTACCTTTCTCACATGG+AGG 0.695492 2.4:+1912468 MS.gene89028:CDS
GTTTGCTGCTTGATTCACAG+TGG 0.700555 2.4:-1910539 None:intergenic
AAGTTGTCGACCGACCATTG+AGG 0.703924 2.4:-1912039 None:intergenic
CAGATGTTTAGCGATACTGA+GGG 0.741969 2.4:-1910631 None:intergenic
CCATTGACGAAAGAGAGGCG+AGG 0.803561 2.4:-1910855 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCTTAAAATTAATAAATA+TGG - chr2.4:1911033-1911052 None:intergenic 10.0%
!! ATTTAATGAATAACATTAAA+AGG + chr2.4:1911346-1911365 MS.gene89028:intron 10.0%
!!! TTTTAATGTTATTCATTAAA+TGG - chr2.4:1911347-1911366 None:intergenic 10.0%
!! TAGTTGTAAATTAAATTCAA+TGG + chr2.4:1911072-1911091 MS.gene89028:intron 15.0%
!! TATTATGATCCTAAATTAAT+TGG - chr2.4:1911816-1911835 None:intergenic 15.0%
!! TTAAATGGAAAGATTAAATT+AGG - chr2.4:1911332-1911351 None:intergenic 15.0%
!!! ATAACTTTCAAATTTTGTTA+TGG + chr2.4:1911258-1911277 MS.gene89028:intron 15.0%
!!! ATCCTTAAATTTTTCATAAA+AGG + chr2.4:1911381-1911400 MS.gene89028:intron 15.0%
!!! TCCTTAAATTTTTCATAAAA+GGG + chr2.4:1911382-1911401 MS.gene89028:intron 15.0%
!!! TTTTAACATCCAATTAATTT+AGG + chr2.4:1911804-1911823 MS.gene89028:intron 15.0%
!! AATCTTCTCTAATTAGAAAT+AGG - chr2.4:1912680-1912699 None:intergenic 20.0%
!! GTGAAACTAATTAGTAATAA+TGG + chr2.4:1911157-1911176 MS.gene89028:intron 20.0%
!! TAAGGAAAAAATAACAAACA+AGG - chr2.4:1911288-1911307 None:intergenic 20.0%
!! TGAAATGGAAAGATTTAATA+AGG - chr2.4:1911235-1911254 None:intergenic 20.0%
!! TTGGATGTTAAAAAATTGTA+AGG - chr2.4:1911797-1911816 None:intergenic 20.0%
!! TTTGAAAGTTATTCATGAAA+TGG - chr2.4:1911250-1911269 None:intergenic 20.0%
!!! AGAAGAAATGAAGTTTTTAT+TGG - chr2.4:1911851-1911870 None:intergenic 20.0%
!!! GAAATATTTTTGAAACAACA+AGG - chr2.4:1912530-1912549 None:intergenic 20.0%
!!! TTATAACTGATTTGTATAAC+TGG + chr2.4:1911935-1911954 MS.gene89028:intron 20.0%
!!! TTTGTATAACTGGTTTTTAA+AGG + chr2.4:1911945-1911964 MS.gene89028:intron 20.0%
! AATCGTGTCTTCTAATTATA+AGG + chr2.4:1910923-1910942 MS.gene89028:CDS 25.0%
! ACTTAAATGTTCATTTGAGT+AGG + chr2.4:1911615-1911634 MS.gene89028:intron 25.0%
! ATAAATATGGCATAAAAGCA+AGG - chr2.4:1911020-1911039 None:intergenic 25.0%
! ATGGTAATTACAACATTAGT+TGG + chr2.4:1911176-1911195 MS.gene89028:intron 25.0%
! ATTTAGAAGGTGAAAATCAT+AGG - chr2.4:1912142-1912161 None:intergenic 25.0%
! GACTAAAGAAGTGAATTTAT+GGG + chr2.4:1911978-1911997 MS.gene89028:CDS 25.0%
! TAACATTAAAAGGACATGAA+TGG + chr2.4:1911356-1911375 MS.gene89028:intron 25.0%
! TAATTAGAAATAGGAAAGGA+TGG - chr2.4:1912671-1912690 None:intergenic 25.0%
! TAGAAAAAAGAATTTCCTTG+AGG + chr2.4:1910563-1910582 MS.gene89028:CDS 25.0%
! TCTCTAATTAGAAATAGGAA+AGG - chr2.4:1912675-1912694 None:intergenic 25.0%
! TGACTAAAGAAGTGAATTTA+TGG + chr2.4:1911977-1911996 MS.gene89028:CDS 25.0%
! TTAAAGAAATTCTTCCTCAA+TGG + chr2.4:1912025-1912044 MS.gene89028:CDS 25.0%
! TTTAGAAGGTGAAAATCATA+GGG - chr2.4:1912141-1912160 None:intergenic 25.0%
! TTTCTTCCAAATGAAAAATG+TGG + chr2.4:1912291-1912310 MS.gene89028:CDS 25.0%
!! GCCCTTTTATGAAAAATTTA+AGG - chr2.4:1911386-1911405 None:intergenic 25.0%
!! TATGATTTTCACCTTCTAAA+TGG + chr2.4:1912141-1912160 MS.gene89028:CDS 25.0%
!! TCTAGAATATGAGAAGTTTA+CGG - chr2.4:1911587-1911606 None:intergenic 25.0%
ATGAGAAGTTTACGGATAAA+CGG - chr2.4:1911579-1911598 None:intergenic 30.0%
ATTGCAGTAGATATGTGTTA+TGG + chr2.4:1911126-1911145 MS.gene89028:intron 30.0%
CTAATTAGAGAAGATTTGAC+TGG + chr2.4:1912684-1912703 MS.gene89028:CDS 30.0%
CTTAATTATGAGCGAGATTA+AGG - chr2.4:1911306-1911325 None:intergenic 30.0%
CTTAGGGCTTATTTATAGAA+GGG + chr2.4:1911653-1911672 MS.gene89028:intron 30.0%
CTTGTGTAAAAAAGAGAGTA+AGG - chr2.4:1911483-1911502 None:intergenic 30.0%
GAAAATACAGAAACATACCT+TGG - chr2.4:1910976-1910995 None:intergenic 30.0%
GTCGAATAGTTGTATTCTAA+AGG - chr2.4:1911708-1911727 None:intergenic 30.0%
GTCTTTCATTGAAGTAAATG+AGG + chr2.4:1912554-1912573 MS.gene89028:CDS 30.0%
TCTAAAGGAGACAATAATGT+TGG - chr2.4:1911693-1911712 None:intergenic 30.0%
TGAAACTTCTGATATGTTGA+AGG + chr2.4:1912434-1912453 MS.gene89028:CDS 30.0%
TTACGTACTATACACCAAAA+TGG - chr2.4:1911460-1911479 None:intergenic 30.0%
TTCATTGAAGTAAATGAGGA+AGG + chr2.4:1912558-1912577 MS.gene89028:CDS 30.0%
! ACTGGAACAATCCTTTTTAT+GGG + chr2.4:1912702-1912721 MS.gene89028:CDS 30.0%
! GAAAACACTATGTTGTTGTT+AGG - chr2.4:1910669-1910688 None:intergenic 30.0%
! GATGATCAATGGATTCAAAT+TGG - chr2.4:1910773-1910792 None:intergenic 30.0%
! GGAATTCATTCTTTTCTCTT+TGG - chr2.4:1910520-1910539 None:intergenic 30.0%
! TTATCTTTTCCTTGCTCATA+AGG - chr2.4:1912249-1912268 None:intergenic 30.0%
!!! GATTTTGAAGTAACACCTTA+GGG + chr2.4:1911637-1911656 MS.gene89028:intron 30.0%
AAAGGAGTTGAGATGATCAA+TGG - chr2.4:1910784-1910803 None:intergenic 35.0%
AAGCTTGTCTTGTAGTAACT+CGG - chr2.4:1912352-1912371 None:intergenic 35.0%
AGAAATTCTTCCTCAATGGT+CGG + chr2.4:1912029-1912048 MS.gene89028:CDS 35.0%
CCAAATGAAACTTCGAATCT+TGG - chr2.4:1912411-1912430 None:intergenic 35.0%
CCAAGATTCGAAGTTTCATT+TGG + chr2.4:1912408-1912427 MS.gene89028:CDS 35.0%
CCTTAGGGCTTATTTATAGA+AGG + chr2.4:1911652-1911671 MS.gene89028:intron 35.0%
CCTTCTATAAATAAGCCCTA+AGG - chr2.4:1911655-1911674 None:intergenic 35.0%
GAAGGAAGGTTGAATAGAAA+GGG - chr2.4:1910898-1910917 None:intergenic 35.0%
GCAAATGCACTGTACTTTAA+AGG + chr2.4:1912078-1912097 MS.gene89028:CDS 35.0%
GGCTTATTTATAGAAGGGAA+TGG + chr2.4:1911658-1911677 MS.gene89028:intron 35.0%
GTAAGATTACCTTTCTCACA+TGG + chr2.4:1912465-1912484 MS.gene89028:CDS 35.0%
TACCTTGGTTACGAAATCAA+CGG - chr2.4:1910961-1910980 None:intergenic 35.0%
TAGAAATAGGAAAGGATGGT+CGG - chr2.4:1912667-1912686 None:intergenic 35.0%
TCACCTTCTAAATGGTAACT+CGG + chr2.4:1912149-1912168 MS.gene89028:CDS 35.0%
TGAAGGAAGGTTGAATAGAA+AGG - chr2.4:1910899-1910918 None:intergenic 35.0%
TGAGAAGTTTACGGATAAAC+GGG - chr2.4:1911578-1911597 None:intergenic 35.0%
TTCTAATTATAAGGCCACTC+TGG + chr2.4:1910932-1910951 MS.gene89028:CDS 35.0%
! AGAAGACACGATTTGTTTGA+AGG - chr2.4:1910916-1910935 None:intergenic 35.0%
! AGTGAATTTATGGGCTGAAA+AGG + chr2.4:1911987-1912006 MS.gene89028:CDS 35.0%
! CACTGTACTTTAAAGGAACA+TGG + chr2.4:1912085-1912104 MS.gene89028:CDS 35.0%
! GACTGGAACAATCCTTTTTA+TGG + chr2.4:1912701-1912720 MS.gene89028:CDS 35.0%
! GATCAATGGATTCAAATTGG+AGG - chr2.4:1910770-1910789 None:intergenic 35.0%
! GGCAAACCACATTTTTCATT+TGG - chr2.4:1912300-1912319 None:intergenic 35.0%
!!! GGATTTTGAAGTAACACCTT+AGG + chr2.4:1911636-1911655 MS.gene89028:intron 35.0%
ACAGATGTTTAGCGATACTG+AGG - chr2.4:1910635-1910654 None:intergenic 40.0%
ACATGGAGGTCTGACAAAAA+TGG + chr2.4:1912482-1912501 MS.gene89028:CDS 40.0%
AGATTACCTTTCTCACATGG+AGG + chr2.4:1912468-1912487 MS.gene89028:CDS 40.0%
ATACACCATTGACGAAAGAG+AGG - chr2.4:1910863-1910882 None:intergenic 40.0%
ATCTCAACTCCTTTGCTTCT+CGG + chr2.4:1910790-1910809 MS.gene89028:CDS 40.0%
CAGATGTTTAGCGATACTGA+GGG - chr2.4:1910634-1910653 None:intergenic 40.0%
CTCTTTCGTCAATGGTGTAT+GGG + chr2.4:1910863-1910882 MS.gene89028:CDS 40.0%
GAGAAGTTTACGGATAAACG+GGG - chr2.4:1911577-1911596 None:intergenic 40.0%
GCTACAAAGTCTAGTCTAGT+TGG - chr2.4:1912645-1912664 None:intergenic 40.0%
GCTGTAACTAAAAGACGTCT+GGG + chr2.4:1912603-1912622 MS.gene89028:CDS 40.0%
TACTGAGGGAAACATTTGCT+TGG - chr2.4:1910620-1910639 None:intergenic 40.0%
TCTAAATGGTAACTCGGTCA+AGG + chr2.4:1912155-1912174 MS.gene89028:CDS 40.0%
TCTCTTTCGTCAATGGTGTA+TGG + chr2.4:1910862-1910881 MS.gene89028:CDS 40.0%
TCTGATATGTTGAAGGAGCT+AGG + chr2.4:1912441-1912460 MS.gene89028:CDS 40.0%
TGACCGAGTTACCATTTAGA+AGG - chr2.4:1912155-1912174 None:intergenic 40.0%
TGAGCACTTGTCCCATAAAA+AGG - chr2.4:1912716-1912735 None:intergenic 40.0%
TGCTGTAACTAAAAGACGTC+TGG + chr2.4:1912602-1912621 MS.gene89028:CDS 40.0%
! AAAATGTGGTTTGCCAGCTT+TGG + chr2.4:1912305-1912324 MS.gene89028:CDS 40.0%
! ATTCACCTACTTCGACTTGT+TGG - chr2.4:1912380-1912399 None:intergenic 40.0%
! GACACGATTTGTTTGAAGGA+AGG - chr2.4:1910912-1910931 None:intergenic 40.0%
! GCTGCTTGATTTTCTTACGA+GGG - chr2.4:1910595-1910614 None:intergenic 40.0%
! TCAAGTTGTTCTCAGCATCA+TGG + chr2.4:1910701-1910720 MS.gene89028:CDS 40.0%
! TTCACCTACTTCGACTTGTT+GGG - chr2.4:1912379-1912398 None:intergenic 40.0%
! TTTGCTGCTTGATTCACAGT+GGG - chr2.4:1910541-1910560 None:intergenic 40.0%
! TTTGTTGTCACGTGACTGAA+AGG - chr2.4:1911534-1911553 None:intergenic 40.0%
!! TGCTGCTTGATTTTCTTACG+AGG - chr2.4:1910596-1910615 None:intergenic 40.0%
!! TTCCGTTGATTTCGTAACCA+AGG + chr2.4:1910956-1910975 MS.gene89028:CDS 40.0%
AATACGTTGCTTCTCCTTGC+TGG - chr2.4:1912193-1912212 None:intergenic 45.0%
ACAACAAGGTCTTGATGTGC+AGG - chr2.4:1912516-1912535 None:intergenic 45.0%
ACTAAAGTTGCCGCACACAA+AGG + chr2.4:1911416-1911435 MS.gene89028:intron 45.0%
AGAACAACTTGAAGCGACAG+TGG - chr2.4:1910693-1910712 None:intergenic 45.0%
CTAAAGTTGCCGCACACAAA+GGG + chr2.4:1911417-1911436 MS.gene89028:intron 45.0%
CTCTGTCTTCCTTATGAGCA+AGG + chr2.4:1912237-1912256 MS.gene89028:CDS 45.0%
GAAGCTTATGAGCGAGACTA+AGG - chr2.4:1911213-1911232 None:intergenic 45.0%
GCATGTAGTGCAGTGAAAAG+AGG - chr2.4:1910998-1911017 None:intergenic 45.0%
GCTACGTTCTTAACCAAAGC+TGG - chr2.4:1912321-1912340 None:intergenic 45.0%
TAAAGTTGCCGCACACAAAG+GGG + chr2.4:1911418-1911437 MS.gene89028:intron 45.0%
TCGAGAAGCTGTTGTTGTGT+GGG - chr2.4:1910744-1910763 None:intergenic 45.0%
TGCATTTGCAAAGATGAGCC+TGG - chr2.4:1912067-1912086 None:intergenic 45.0%
TGCGGCAACTTTAGTGCAAT+GGG - chr2.4:1911411-1911430 None:intergenic 45.0%
TGGAGGTCTGACAAAAATGG+TGG + chr2.4:1912485-1912504 MS.gene89028:CDS 45.0%
TGGGCTGAAAAGGAGACAAA+TGG + chr2.4:1911997-1912016 MS.gene89028:CDS 45.0%
TTACGAGGGAAACATCCTCA+AGG - chr2.4:1910581-1910600 None:intergenic 45.0%
TTCTCCTTGCTGGTCATGAA+GGG - chr2.4:1912183-1912202 None:intergenic 45.0%
! AGCAGCATCTTTGAGGATGA+TGG - chr2.4:1910823-1910842 None:intergenic 45.0%
! GTTTGCTGCTTGATTCACAG+TGG - chr2.4:1910542-1910561 None:intergenic 45.0%
AAGTTGTCGACCGACCATTG+AGG - chr2.4:1912042-1912061 None:intergenic 50.0%
CCTTCCCAACAAGTCGAAGT+AGG + chr2.4:1912372-1912391 MS.gene89028:CDS 50.0%
CTTCTCCTTGCTGGTCATGA+AGG - chr2.4:1912184-1912203 None:intergenic 50.0%
GAAATCAACGGAAGCCAGAG+TGG - chr2.4:1910949-1910968 None:intergenic 50.0%
GTCAGACCTCCATGTGAGAA+AGG - chr2.4:1912477-1912496 None:intergenic 50.0%
GTCGAGAAGCTGTTGTTGTG+TGG - chr2.4:1910745-1910764 None:intergenic 50.0%
GTGCGGCAACTTTAGTGCAA+TGG - chr2.4:1911412-1911431 None:intergenic 50.0%
GTTCTCAGCATCATGGTTGC+AGG + chr2.4:1910708-1910727 MS.gene89028:CDS 50.0%
! ATCATGGTTGCAGGCTCTGA+GGG + chr2.4:1910717-1910736 MS.gene89028:CDS 50.0%
! GGGAAGGAGTCACACCATTT+TGG + chr2.4:1911443-1911462 MS.gene89028:intron 50.0%
! TACGGATAAACGGGGAGCAT+TGG - chr2.4:1911569-1911588 None:intergenic 50.0%
!! CCTACTTCGACTTGTTGGGA+AGG - chr2.4:1912375-1912394 None:intergenic 50.0%
AAAGATGCTGCTCCTGCAGG+TGG + chr2.4:1910831-1910850 MS.gene89028:CDS 55.0%
CATCATGGTTGCAGGCTCTG+AGG + chr2.4:1910716-1910735 MS.gene89028:CDS 55.0%
CCATTGACGAAAGAGAGGCG+AGG - chr2.4:1910858-1910877 None:intergenic 55.0%
CCTCGCCTCTCTTTCGTCAA+TGG + chr2.4:1910855-1910874 MS.gene89028:CDS 55.0%
CTCAAAGATGCTGCTCCTGC+AGG + chr2.4:1910828-1910847 MS.gene89028:CDS 55.0%
CTGCAGGAGCAGCATCTTTG+AGG - chr2.4:1910830-1910849 None:intergenic 55.0%
CTTCCCATCCCCTTTGTGTG+CGG - chr2.4:1911429-1911448 None:intergenic 55.0%
GGAAACGAGCCGAGAAGCAA+AGG - chr2.4:1910802-1910821 None:intergenic 55.0%
GGTCGGTCGACAACTTAACC+AGG + chr2.4:1912046-1912065 MS.gene89028:CDS 55.0%
GGTTCCCTTCATGACCAGCA+AGG + chr2.4:1912176-1912195 MS.gene89028:CDS 55.0%
TTGCCGCACACAAAGGGGAT+GGG + chr2.4:1911423-1911442 MS.gene89028:intron 55.0%
CGCACACAAAGGGGATGGGA+AGG + chr2.4:1911427-1911446 MS.gene89028:intron 60.0%
GTTGCCGCACACAAAGGGGA+TGG + chr2.4:1911422-1911441 MS.gene89028:intron 60.0%
!! AACGGGGAGCATTGGCGCAA+CGG - chr2.4:1911561-1911580 None:intergenic 60.0%
GAGAGGCGAGGTCCACCTGC+AGG - chr2.4:1910846-1910865 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 1910513 1912758 1910513 ID=MS.gene89028
chr2.4 mRNA 1910513 1912758 1910513 ID=MS.gene89028.t1;Parent=MS.gene89028
chr2.4 exon 1910513 1910977 1910513 ID=MS.gene89028.t1.exon1;Parent=MS.gene89028.t1
chr2.4 CDS 1910513 1910977 1910513 ID=cds.MS.gene89028.t1;Parent=MS.gene89028.t1
chr2.4 exon 1911967 1912758 1911967 ID=MS.gene89028.t1.exon2;Parent=MS.gene89028.t1
chr2.4 CDS 1911967 1912758 1911967 ID=cds.MS.gene89028.t1;Parent=MS.gene89028.t1
Gene Sequence

>MS.gene89028

ATGACCAAAGAGAAAAGAATGAATTCCCACTGTGAATCAAGCAGCAAACGTAGAAAAAAGAATTTCCTTGAGGATGTTTCCCTCGTAAGAAAATCAAGCAGCAACCAAGCAAATGTTTCCCTCAGTATCGCTAAACATCTGTTCGCGAAACAACCTAACAACAACATAGTGTTTTCTCCACTGTCGCTTCAAGTTGTTCTCAGCATCATGGTTGCAGGCTCTGAGGGTCCCACACAACAACAGCTTCTCGACTTCCTCCAATTTGAATCCATTGATCATCTCAACTCCTTTGCTTCTCGGCTCGTTTCCATCATCCTCAAAGATGCTGCTCCTGCAGGTGGACCTCGCCTCTCTTTCGTCAATGGTGTATGGGTAGATAAAACCCTTTCTATTCAACCTTCCTTCAAACAAATCGTGTCTTCTAATTATAAGGCCACTCTGGCTTCCGTTGATTTCGTAACCAAGGTATGTTTCTGTATTTTCCTCTTTTCACTGCACTACATGCCTTGCTTTTATGCCATATTTATTAATTTTAAGCTTAGTTAGTGTTATGCATTATTAGTTGTAAATTAAATTCAATGGCTAGATGTATATCATTAGTATTAGTATTGCTATTGCAGTAGATATGTGTTATGGTATGTTTTGTGAAACTAATTAGTAATAATGGTAATTACAACATTAGTTGGTTCTGTGAGTTCCTTAGTCTCGCTCATAAGCTTCCTTATTAAATCTTTCCATTTCATGAATAACTTTCAAATTTTGTTATGGTATGCCTTGTTTGTTATTTTTTCCTTAATCTCGCTCATAATTAAGCTTCCTAATTTAATCTTTCCATTTAATGAATAACATTAAAAGGACATGAATGGAGATCCTTAAATTTTTCATAAAAGGGCATCCCATTGCACTAAAGTTGCCGCACACAAAGGGGATGGGAAGGAGTCACACCATTTTGGTGTATAGTACGTAACCTTACTCTCTTTTTTACACAAGAGATTGTTTTCAAAACTTGAATCTGTGACCTTTCAGTCACGTGACAACAAATTTACCGTTGCGCCAATGCTCCCCGTTTATCCGTAAACTTCTCATATTCTAGAATTTTGAAACTTAAATGTTCATTTGAGTAGGATTTTGAAGTAACACCTTAGGGCTTATTTATAGAAGGGAATGGTGATTATTACCAACATTATTGTCTCCTTTAGAATACAACTATTCGACTAAAATGAACTTAGTGCAACAAGTATCAACTTCTACGAAATCAATTGATGAATGCAACTTTTCTCTCCTTACAATTTTTTAACATCCAATTAATTTAGGATCATAATATATGAAAATATACCAATAAAAACTTCATTTCTTCTATTGAAATATTAGTGAAAAGAATTCAACTAATCTATTTGCATAACTAATTTTTAGACTTATAAGTTATAACTGATTTGTATAACTGGTTTTTAAAGGCTACTGAAGTGACTAAAGAAGTGAATTTATGGGCTGAAAAGGAGACAAATGGTCTTATTAAAGAAATTCTTCCTCAATGGTCGGTCGACAACTTAACCAGGCTCATCTTTGCAAATGCACTGTACTTTAAAGGAACATGGAATCAGAAGTTTGACGCTTCGACAACAAAACCCTATGATTTTCACCTTCTAAATGGTAACTCGGTCAAGGTTCCCTTCATGACCAGCAAGGAGAAGCAACGTATTAGTGCTTTTAATGATTTTAAAGTTCTCTGTCTTCCTTATGAGCAAGGAAAAGATAAACGTCAAATCTCTATGTACATTTTTCTTCCAAATGAAAAATGTGGTTTGCCAGCTTTGGTTAAGAACGTAGCTTCTAAATCCGAGTTACTACAAGACAAGCTTCCTTCCCAACAAGTCGAAGTAGGTGAATTTAGAATTCCAAGATTCGAAGTTTCATTTGGACTTGAAACTTCTGATATGTTGAAGGAGCTAGGAGTAAGATTACCTTTCTCACATGGAGGTCTGACAAAAATGGTGGACTCTCCTGCACATCAAGACCTTGTTGTTTCAAAAATATTTCAAAAGTCTTTCATTGAAGTAAATGAGGAAGGCACTGAAGCTGCTGTAGTCAGTGCTGTAACTAAAAGACGTCTGGGTTTGAGTATGCGCACTCCAACTAGACTAGACTTTGTAGCCGACCATCCTTTCCTATTTCTAATTAGAGAAGATTTGACTGGAACAATCCTTTTTATGGGACAAGTGCTCAATCCTCTTGCTGAAAGATCCTAA
Protein sequence

>MS.gene89028.t1

MTKEKRMNSHCESSSKRRKKNFLEDVSLVRKSSSNQANVSLSIAKHLFAKQPNNNIVFSPLSLQVVLSIMVAGSEGPTQQQLLDFLQFESIDHLNSFASRLVSIILKDAAPAGGPRLSFVNGVWVDKTLSIQPSFKQIVSSNYKATLASVDFVTKATEVTKEVNLWAEKETNGLIKEILPQWSVDNLTRLIFANALYFKGTWNQKFDASTTKPYDFHLLNGNSVKVPFMTSKEKQRISAFNDFKVLCLPYEQGKDKRQISMYIFLPNEKCGLPALVKNVASKSELLQDKLPSQQVEVGEFRIPRFEVSFGLETSDMLKELGVRLPFSHGGLTKMVDSPAHQDLVVSKIFQKSFIEVNEEGTEAAVVSAVTKRRLGLSMRTPTRLDFVADHPFLFLIREDLTGTILFMGQVLNPLAERS