AlfalfaGEDB | MS.gene89659

M. sativa cultivar XinJiangDaYe / MS.gene89659

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00514.26

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89659.t1	XP_003629314.1	94.1	674	32	3	1	673	1	667	0.00E+00	1152.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89659.t1	B7FF18	94.1	674	32	3	1	673	1	667	0.0e+00	1152.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050551	MS.gene89659	0.806315	8.73E-50	-1.69E-46
MS.gene052609	MS.gene89659	0.802291	6.02E-49	-1.69E-46
MS.gene058758	MS.gene89659	0.800691	1.28E-48	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89659.t1	MTR_8g075770	97.329	674	10	3	1	673	1	667	0.0	1324
MS.gene89659.t1	MTR_5g022240	71.014	690	180	8	1	673	1	687	0.0	936
MS.gene89659.t1	MTR_4g073830	60.358	671	246	6	1	669	1	653	0.0	809
MS.gene89659.t1	MTR_5g033190	36.556	662	338	11	9	662	14	601	1.59e-115	359
MS.gene89659.t1	MTR_0064s0050	57.143	217	91	2	1	216	1	216	6.22e-69	225
MS.gene89659.t1	MTR_0064s0010	54.808	208	92	2	1	207	1	207	1.10e-64	214
MS.gene89659.t1	MTR_1g046850	53.241	216	90	4	5	219	8	213	4.01e-62	211
MS.gene89659.t1	MTR_4g105110	39.689	257	151	4	408	662	328	582	3.60e-46	173
MS.gene89659.t1	MTR_4g105110	36.129	310	170	9	12	307	23	318	1.09e-39	154
MS.gene89659.t1	MTR_4g105110	39.689	257	151	4	408	662	329	583	4.10e-46	173

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89659.t1	AT5G66200	68.107	671	188	7	1	668	1	648	0.0	877
MS.gene89659.t1	AT4G34940	64.948	679	213	7	1	669	1	664	0.0	850
MS.gene89659.t1	AT4G36030	60.088	679	251	10	1	669	1	669	0.0	750
MS.gene89659.t1	AT3G26600	34.709	654	342	10	21	662	23	603	2.09e-102	325
MS.gene89659.t1	AT3G26600	40.074	272	151	3	392	662	262	522	1.78e-47	176
MS.gene89659.t1	AT3G26600	43.814	194	101	3	21	206	23	216	4.92e-40	154

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Find 144 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTCCTGCTTGTAAAG+CGG	0.627963	8.3:+27974887	MS.gene89659:CDS
AAATCAGATGCATAAAGTTG+TGG	0.616774	8.3:+27974431	MS.gene89659:CDS
AAATTGATTATTGAAGAGGG+TGG	0.676552	8.3:+27973949	MS.gene89659:CDS
AACAAGCAACAAACCAATGA+AGG	0.467751	8.3:+27974486	MS.gene89659:CDS
AACAAGGATAATTGGTCCGT+TGG	0.519943	8.3:+27975013	MS.gene89659:CDS
AAGCAAGGCTATTCGTATTC+TGG	0.315669	8.3:+27974603	MS.gene89659:CDS
AAGGCAATTATAAGCTTTGG+TGG	0.523348	8.3:+27975137	MS.gene89659:CDS
AAGGCTGAATATTGAACTGG+CGG	0.644602	8.3:-27975197	None:intergenic
AAGGTAGCTATGAATGTCTT+GGG	0.470872	8.3:+27973785	MS.gene89659:CDS
AAGGTGAAGGTAGTTATCAT+TGG	0.490542	8.3:-27975105	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCAGATGCATAAAGTTG+TGG	+	chr8.3:27974431-27974450	MS.gene89659:CDS	30.0%
AAATTGATTATTGAAGAGGG+TGG	+	chr8.3:27973949-27973968	MS.gene89659:CDS	30.0%
AGTGAAGTATAATTCTGCTT+TGG	+	chr8.3:27974800-27974819	MS.gene89659:CDS	30.0%
ATTCCTTGTATCAAGGTTAT+TGG	+	chr8.3:27974960-27974979	MS.gene89659:CDS	30.0%
CTCATCTTGAGAATTCAATT+GGG	+	chr8.3:27973722-27973741	MS.gene89659:CDS	30.0%
GAATTATACTTCACTTCTTG+AGG	-	chr8.3:27974795-27974814	None:intergenic	30.0%
GCAAAAACATCTTGACATTT+AGG	-	chr8.3:27974189-27974208	None:intergenic	30.0%
GGGAAATTGATTATTGAAGA+GGG	+	chr8.3:27973946-27973965	MS.gene89659:CDS	30.0%
TCATCTTGAGAATTCAATTG+GGG	+	chr8.3:27973723-27973742	MS.gene89659:CDS	30.0%
TCCCAAATTAAACCCAAAAT+CGG	-	chr8.3:27973835-27973854	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	27973403	27975424	27973403	ID=MS.gene89659
chr8.3	mRNA	27973403	27975424	27973403	ID=MS.gene89659.t1;Parent=MS.gene89659
chr8.3	exon	27973403	27975424	27973403	ID=MS.gene89659.t1.exon1;Parent=MS.gene89659.t1
chr8.3	CDS	27973403	27975424	27973403	ID=cds.MS.gene89659.t1;Parent=MS.gene89659.t1

Gene Sequence

>MS.gene89659

ATGGCAGACATAGTGAAACAATTGTTAGCAAAACCGATCCAACTAGCTGACCAAGTAAGCAAAGCAGCAGAAGAAGGAAGCTCATCTTTCAAACAAGAATGTCTTGACCTCAAATCCAAAACCGAAAAACTCGCTTCTCTTCTTCGACAAGCCGCAAGATCAAGCTCCGATTTATACGAACGACCCACACGTCGTATCATCGGAGACACCGAACAGGTTCTTGAAAAAGCACTCACTTTAGTCCTTAAATGCAAAGTCAACGGTCTCATGAAACGTGTTTTCAGCATAGTTCCTGCTGCAGCTTTTCGTAAAATGTCTTCTCATCTTGAGAATTCAATTGGGGATGTTTCATGGCTTCTTCGAGTTTCTGCTCCGGCTGAAGAAGGTAGCTATGAATGTCTTGGGCTGCCACCAATTGCTTCAAATGAACCGATTTTGGGTTTAATTTGGGAGCAGATTGCGATTTTACATAATGGGTCGTTTGATGATCGATCTGATGCTGCTGCTTCTTTGGTTTCTCTTGTTCGTGATAATGATCGGAATGGGAAATTGATTATTGAAGAGGGTGGTGTTGGGCCTTTGCTGAAACTTTTGAAAGAAGGTAAGAAAGAAGGGCAAGAGAATGCTGCAAAAGCAATTGGGTTATTGGGTCGTGATCCGGAGAGTGTTGAGGTTATGATTCATGCTGGTGTTTGTTCTGTTTTCGCGAAGATTCTTAAAGAAGGTCCTATGAAAGTTCAAGCTGTTGTAGCTTGGGCTGTTTCTGAACTTGTTTCTAAGTATCCTAAATGTCAAGATGTTTTTGCTCAGCATAATATTGTTAGGTTGCTTGTTAGTCATATTGCCTTTGAAACTGTTCAGGAACATAGTAAATATGCAATTGTTAGTAATAAGGCTATTTCTTCGATTCATGCTGTTGTGTTGGCTAGTGGTAATAATAATAATAATTCTGATTCAAATGATGTGAAAAAAGAGAACGACGATGAGGTGAAAATTAAGATGCAGCATCCTTTAGGTGATAAGTCTGCAAATCAGATGCATAAAGTTGTGGCAAGTACTATGGCAATGCATGCTGCGAATAATAACAAGCAACAAACCAATGAAGGGAGTAAATTGAGTTTAAACTCTCAACCTCCGGTTCATGTGGCTAGTATTCCAAATGGGAATGGGAATGGGAATGGTAATGGTAATGGTAACACGAAGCAAGGCTATTCGTATTCTGGAATTAACATGAAGGGAAGGGAGCTTGAGGATGCCGAGAGTAAGGCTGACATGAAAGCAATGGCTGCGAAAGCTCTTAGGTATTTAGCTAAGGGTAACTCGTCGATTTGTCGTAGCATAACTGAATCAAGGGCTTTGTTGTGTTTTGCTATTTTACTTGAAAAAGGGCCTCAAGAAGTGAAGTATAATTCTGCTTTGGCATTGAAAGAGATTACTGCAGTGGCTGAGAAAGATCCTGAATTGAGAAGATCGGCATTTAAGCCAAACACTCCTGCTTGTAAAGCGGTTGTTGATCAAGTGATTGATATTATTGACAAAGAAGATGAGAGACTTTTGATTCCTTGTATCAAGGTTATTGGGAGTTTGGCCAGGACATTCAGGGCAACTGAAACAAGGATAATTGGTCCGTTGGTTCGACTTCTTGATGAAAGAGAGGCTGAAGTATCAAAAGAGGCTGCAGACTCACTCGCGAAATTCGCTTCCAATGATAACTACCTTCACCTTGATCACTGTAAGGCAATTATAAGCTTTGGTGGTGTAAAACCCTTGGTTCAGCTTGTGTATCTAGGGGAGCCGCCAGTTCAATATTCAGCCTTGGTTCTGCTATCCTATATTGCGTTGCACGTGCCAGACAGTGAAGAACTAGCAAAAGCTGAGATTCTGGGAGTTCTTGAATGGGCATCTAAGCAACCTAACATGGCTCATGATGAAGCTATTGAGGCATTGTTGCAAGAATCCAAGAGTAGGCTGGAGCTTTACCAGTCTAGAGGTTCACGAGGATTCCAAAAATTACATCAATAG

Protein sequence

>MS.gene89659.t1

MADIVKQLLAKPIQLADQVSKAAEEGSSSFKQECLDLKSKTEKLASLLRQAARSSSDLYERPTRRIIGDTEQVLEKALTLVLKCKVNGLMKRVFSIVPAAAFRKMSSHLENSIGDVSWLLRVSAPAEEGSYECLGLPPIASNEPILGLIWEQIAILHNGSFDDRSDAAASLVSLVRDNDRNGKLIIEEGGVGPLLKLLKEGKKEGQENAAKAIGLLGRDPESVEVMIHAGVCSVFAKILKEGPMKVQAVVAWAVSELVSKYPKCQDVFAQHNIVRLLVSHIAFETVQEHSKYAIVSNKAISSIHAVVLASGNNNNNSDSNDVKKENDDEVKIKMQHPLGDKSANQMHKVVASTMAMHAANNNKQQTNEGSKLSLNSQPPVHVASIPNGNGNGNGNGNGNTKQGYSYSGINMKGRELEDAESKADMKAMAAKALRYLAKGNSSICRSITESRALLCFAILLEKGPQEVKYNSALALKEITAVAEKDPELRRSAFKPNTPACKAVVDQVIDIIDKEDERLLIPCIKVIGSLARTFRATETRIIGPLVRLLDEREAEVSKEAADSLAKFASNDNYLHLDHCKAIISFGGVKPLVQLVYLGEPPVQYSALVLLSYIALHVPDSEELAKAEILGVLEWASKQPNMAHDEAIEALLQESKSRLELYQSRGSRGFQKLHQ