Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89720.t1 | XP_013447096.1 | 93.8 | 144 | 8 | 1 | 1 | 143 | 177 | 320 | 6.00E-72 | 280 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89720.t1 | A0A072TW59 | 93.8 | 144 | 8 | 1 | 1 | 143 | 177 | 320 | 4.4e-72 | 280.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene06002 | MS.gene89720 | 0.912099 | 2.87E-83 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89720.t1 | MTR_8g098860 | 93.750 | 144 | 8 | 1 | 1 | 143 | 177 | 320 | 1.74e-94 | 276 |
| MS.gene89720.t1 | MTR_5g010020 | 43.902 | 164 | 55 | 11 | 7 | 136 | 177 | 337 | 1.19e-19 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCACCAAATTCATCAAATA+TGG | 0.189419 | 4.4:+5327237 | MS.gene89720:CDS |
| GTTCCCATATTTGATGAATT+TGG | 0.278649 | 4.4:-5327241 | None:intergenic |
| GAGCCCGGTGATTCATTTCA+TGG | 0.344990 | 4.4:+5326896 | MS.gene89720:CDS |
| TTGGAGGAGGTAGTGATGAT+TGG | 0.377550 | 4.4:-5327009 | None:intergenic |
| TTCTGGGACAATATTGTTCA+AGG | 0.397659 | 4.4:+5327187 | MS.gene89720:CDS |
| TTATACCGTTTATAGAATCT+TGG | 0.426214 | 4.4:-5326964 | None:intergenic |
| TACTTCCAAGATTCTATAAA+CGG | 0.434921 | 4.4:+5326959 | MS.gene89720:CDS |
| TAGAATGCTTGCAGAGTCAA+TGG | 0.439026 | 4.4:+5327128 | MS.gene89720:CDS |
| ATTGTTCAAGGGCATGGACT+TGG | 0.450777 | 4.4:+5327199 | MS.gene89720:CDS |
| CCACCAAATTCATCAAATAT+GGG | 0.474503 | 4.4:+5327238 | MS.gene89720:CDS |
| GTTCAAGGGCATGGACTTGG+TGG | 0.480608 | 4.4:+5327202 | MS.gene89720:CDS |
| CTAAAAGAAGAAGAAGGAAA+AGG | 0.490998 | 4.4:-5327283 | None:intergenic |
| TTCAAGGGCATGGACTTGGT+GGG | 0.505959 | 4.4:+5327203 | MS.gene89720:CDS |
| GACAATATTGTTCAAGGGCA+TGG | 0.513323 | 4.4:+5327193 | MS.gene89720:CDS |
| AAAGTTGTTGATATTGGAGG+AGG | 0.535605 | 4.4:-5327022 | None:intergenic |
| TGTTCCATGAAATGAATCAC+CGG | 0.561446 | 4.4:-5326900 | None:intergenic |
| TCAAGGGCATGGACTTGGTG+GGG | 0.562532 | 4.4:+5327204 | MS.gene89720:CDS |
| CCCATATTTGATGAATTTGG+TGG | 0.563290 | 4.4:-5327238 | None:intergenic |
| TCCCAGAAAAGAAAGTTACT+AGG | 0.564527 | 4.4:-5327172 | None:intergenic |
| AGAATCTTGGAAGTATTGAT+CGG | 0.567267 | 4.4:-5326951 | None:intergenic |
| ACGGTATAAGCTCTGAAACA+TGG | 0.571232 | 4.4:+5326978 | MS.gene89720:CDS |
| TCTGGGACAATATTGTTCAA+GGG | 0.578297 | 4.4:+5327188 | MS.gene89720:CDS |
| TTATCTCTAAAAGAAGAAGA+AGG | 0.581455 | 4.4:-5327289 | None:intergenic |
| ACCACCTTTGATAGAGAATG+TGG | 0.583136 | 4.4:+5327069 | MS.gene89720:CDS |
| CCTTTGTTTATGTTTGAGCC+CGG | 0.594664 | 4.4:+5326881 | None:intergenic |
| TCCACATTCTCTATCAAAGG+TGG | 0.596829 | 4.4:-5327070 | None:intergenic |
| AGAATGCTTGCAGAGTCAAT+GGG | 0.603509 | 4.4:+5327129 | MS.gene89720:CDS |
| GTGGAAAACATGGACGAGGA+AGG | 0.617283 | 4.4:+5327088 | MS.gene89720:CDS |
| GACGAGGAAGGAGATCATAG+AGG | 0.622748 | 4.4:+5327100 | MS.gene89720:CDS |
| GATAGAGAATGTGGAAAACA+TGG | 0.635293 | 4.4:+5327078 | MS.gene89720:CDS |
| GTTCCATGAAATGAATCACC+GGG | 0.637744 | 4.4:-5326899 | None:intergenic |
| GAATGTGGAAAACATGGACG+AGG | 0.646679 | 4.4:+5327084 | MS.gene89720:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAAAAGTTGTTGATAT+TGG | - | chr4.4:5327031-5327050 | None:intergenic | 20.0% |
| !!! | AATATCAACAACTTTTTCAA+TGG | + | chr4.4:5327030-5327049 | MS.gene89720:CDS | 20.0% |
| ! | TACTTCCAAGATTCTATAAA+CGG | + | chr4.4:5326959-5326978 | MS.gene89720:CDS | 25.0% |
| ! | TTATACCGTTTATAGAATCT+TGG | - | chr4.4:5326967-5326986 | None:intergenic | 25.0% |
| !! | TTATCTCTAAAAGAAGAAGA+AGG | - | chr4.4:5327292-5327311 | None:intergenic | 25.0% |
| ACCACCAAATTCATCAAATA+TGG | + | chr4.4:5327237-5327256 | MS.gene89720:CDS | 30.0% | |
| CCACCAAATTCATCAAATAT+GGG | + | chr4.4:5327238-5327257 | MS.gene89720:CDS | 30.0% | |
| CTAAAAGAAGAAGAAGGAAA+AGG | - | chr4.4:5327286-5327305 | None:intergenic | 30.0% | |
| GAAAAAGTTGTTGATATTGG+AGG | - | chr4.4:5327028-5327047 | None:intergenic | 30.0% | |
| GTTCCCATATTTGATGAATT+TGG | - | chr4.4:5327244-5327263 | None:intergenic | 30.0% | |
| ! | TCCTAGTAACTTTCTTTTCT+GGG | + | chr4.4:5327171-5327190 | MS.gene89720:CDS | 30.0% |
| ! | TTTTCCACATTCTCTATCAA+AGG | - | chr4.4:5327076-5327095 | None:intergenic | 30.0% |
| !! | AGAATCTTGGAAGTATTGAT+CGG | - | chr4.4:5326954-5326973 | None:intergenic | 30.0% |
| AAAGTTGTTGATATTGGAGG+AGG | - | chr4.4:5327025-5327044 | None:intergenic | 35.0% | |
| CCCATATTTGATGAATTTGG+TGG | - | chr4.4:5327241-5327260 | None:intergenic | 35.0% | |
| GATAGAGAATGTGGAAAACA+TGG | + | chr4.4:5327078-5327097 | MS.gene89720:CDS | 35.0% | |
| TCCCAGAAAAGAAAGTTACT+AGG | - | chr4.4:5327175-5327194 | None:intergenic | 35.0% | |
| TGTTCCATGAAATGAATCAC+CGG | - | chr4.4:5326903-5326922 | None:intergenic | 35.0% | |
| ! | GTCCTAGTAACTTTCTTTTC+TGG | + | chr4.4:5327170-5327189 | MS.gene89720:CDS | 35.0% |
| ! | TCTGGGACAATATTGTTCAA+GGG | + | chr4.4:5327188-5327207 | MS.gene89720:CDS | 35.0% |
| !! | TTCTGGGACAATATTGTTCA+AGG | + | chr4.4:5327187-5327206 | MS.gene89720:CDS | 35.0% |
| ACCACCTTTGATAGAGAATG+TGG | + | chr4.4:5327069-5327088 | MS.gene89720:CDS | 40.0% | |
| AGAATGCTTGCAGAGTCAAT+GGG | + | chr4.4:5327129-5327148 | MS.gene89720:CDS | 40.0% | |
| GACAATATTGTTCAAGGGCA+TGG | + | chr4.4:5327193-5327212 | MS.gene89720:CDS | 40.0% | |
| GTTCCATGAAATGAATCACC+GGG | - | chr4.4:5326902-5326921 | None:intergenic | 40.0% | |
| TAGAATGCTTGCAGAGTCAA+TGG | + | chr4.4:5327128-5327147 | MS.gene89720:CDS | 40.0% | |
| TCCACATTCTCTATCAAAGG+TGG | - | chr4.4:5327073-5327092 | None:intergenic | 40.0% | |
| !! | ACGGTATAAGCTCTGAAACA+TGG | + | chr4.4:5326978-5326997 | MS.gene89720:CDS | 40.0% |
| GAATGTGGAAAACATGGACG+AGG | + | chr4.4:5327084-5327103 | MS.gene89720:CDS | 45.0% | |
| TTGGAGGAGGTAGTGATGAT+TGG | - | chr4.4:5327012-5327031 | None:intergenic | 45.0% | |
| ! | ATTGTTCAAGGGCATGGACT+TGG | + | chr4.4:5327199-5327218 | MS.gene89720:CDS | 45.0% |
| GACGAGGAAGGAGATCATAG+AGG | + | chr4.4:5327100-5327119 | MS.gene89720:CDS | 50.0% | |
| GAGCCCGGTGATTCATTTCA+TGG | + | chr4.4:5326896-5326915 | MS.gene89720:CDS | 50.0% | |
| GTGGAAAACATGGACGAGGA+AGG | + | chr4.4:5327088-5327107 | MS.gene89720:CDS | 50.0% | |
| ! | TTCAAGGGCATGGACTTGGT+GGG | + | chr4.4:5327203-5327222 | MS.gene89720:CDS | 50.0% |
| ! | GTTCAAGGGCATGGACTTGG+TGG | + | chr4.4:5327202-5327221 | MS.gene89720:CDS | 55.0% |
| !! | TCAAGGGCATGGACTTGGTG+GGG | + | chr4.4:5327204-5327223 | MS.gene89720:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 5326890 | 5327321 | 5326890 | ID=MS.gene89720 |
| chr4.4 | mRNA | 5326890 | 5327321 | 5326890 | ID=MS.gene89720.t1;Parent=MS.gene89720 |
| chr4.4 | exon | 5326890 | 5327321 | 5326890 | ID=MS.gene89720.t1.exon1;Parent=MS.gene89720.t1 |
| chr4.4 | CDS | 5326890 | 5327321 | 5326890 | ID=cds.MS.gene89720.t1;Parent=MS.gene89720.t1 |
| Gene Sequence |
| Protein sequence |
