AlfalfaGEDB | MS.gene90094

M. sativa cultivar XinJiangDaYe / MS.gene90094

KEGG: K03768
GO: GO:0000413 GO:0003674 GO:0003755 GO:0003824 GO:0006457 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016859 GO:0018193 GO:0018208 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00160.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90094.t1	XP_013470516.1	97.9	97	2	0	42	138	51	147	6.00E-45	190.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90094.t1	Q9ASS6	78.8	85	18	0	54	138	76	160	6.9e-34	145.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90094.t1	I3S7Z5	97.9	97	2	0	42	138	51	147	4.3e-45	190.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053836	MS.gene90094	0.800251	1.57E-48	-1.69E-46
MS.gene055422	MS.gene90094	0.807038	6.14E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90094.t1	MTR_1g117010	87.755	147	6	2	2	138	3	147	1.30e-81	242
MS.gene90094.t1	MTR_1g117010	87.755	147	6	2	2	138	3	147	1.30e-81	242
MS.gene90094.t1	MTR_1g085560	67.391	92	27	1	54	145	71	159	3.22e-34	121
MS.gene90094.t1	MTR_7g110980	53.535	99	43	1	40	138	68	163	1.56e-29	109
MS.gene90094.t1	MTR_3g463900	56.818	88	28	2	58	138	24	108	9.37e-24	93.2
MS.gene90094.t1	MTR_3g070500	48.246	114	35	4	46	138	28	138	1.83e-23	91.3
MS.gene90094.t1	MTR_3g070500	48.246	114	35	4	46	138	28	138	2.39e-23	90.9
MS.gene90094.t1	MTR_3g070500	48.246	114	35	4	46	138	28	138	3.51e-23	91.3
MS.gene90094.t1	MTR_3g070500	48.246	114	35	4	46	138	28	138	8.74e-23	91.3
MS.gene90094.t1	MTR_3g117750	57.143	77	23	2	69	138	13	86	2.57e-21	86.3
MS.gene90094.t1	MTR_4g075290	55.000	80	26	2	66	138	2	78	1.07e-19	81.6
MS.gene90094.t1	MTR_7g116340	54.545	77	25	2	69	138	5	78	2.95e-19	80.5
MS.gene90094.t1	MTR_4g073650	48.235	85	34	2	56	133	34	115	5.42e-19	80.9
MS.gene90094.t1	MTR_8g079690	58.904	73	19	2	69	133	5	74	2.96e-18	80.9
MS.gene90094.t1	MTR_7g095750	54.762	84	27	2	70	145	9	89	4.25e-18	81.3
MS.gene90094.t1	MTR_8g040180	48.352	91	39	2	53	138	3	90	1.75e-16	73.6
MS.gene90094.t1	MTR_4g086760	47.436	78	30	2	69	138	5	79	4.47e-15	71.6
MS.gene90094.t1	MTR_4g086760	47.436	78	30	2	69	138	5	79	5.00e-15	72.0
MS.gene90094.t1	MTR_4g086760	47.436	78	30	2	69	138	5	79	5.62e-15	71.6
MS.gene90094.t1	MTR_3g107660	46.575	73	28	2	70	134	12	81	1.96e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90094.t1	AT5G13120	78.824	85	18	0	54	138	76	160	4.00e-44	147
MS.gene90094.t1	AT5G13120	78.824	85	18	0	54	138	72	156	4.18e-44	147
MS.gene90094.t1	AT3G62030	57.692	104	40	2	43	145	69	169	2.71e-33	119
MS.gene90094.t1	AT3G62030	56.311	103	42	1	43	145	69	168	3.21e-33	119
MS.gene90094.t1	AT3G62030	57.692	104	40	2	43	145	122	222	9.81e-33	119
MS.gene90094.t1	AT5G58710	60.494	81	22	2	65	138	32	109	3.89e-25	96.7
MS.gene90094.t1	AT2G29960	61.728	81	21	2	65	138	29	106	1.65e-24	95.1
MS.gene90094.t1	AT2G29960	61.728	81	21	2	65	138	29	106	1.65e-24	95.1
MS.gene90094.t1	AT2G29960	61.728	81	21	2	65	138	29	106	1.92e-24	94.7
MS.gene90094.t1	AT3G55920	46.903	113	36	5	44	138	27	133	7.15e-22	88.6
MS.gene90094.t1	AT4G34960	48.515	101	42	2	52	145	31	128	8.11e-21	84.7
MS.gene90094.t1	AT2G16600	54.545	77	25	2	69	138	6	79	1.10e-20	84.3
MS.gene90094.t1	AT4G34960	45.833	120	54	3	52	164	31	146	2.23e-20	84.7
MS.gene90094.t1	AT2G21130	55.844	77	24	2	69	138	6	79	2.60e-20	83.6
MS.gene90094.t1	AT4G38740	53.247	77	26	2	69	138	5	78	2.66e-18	78.2
MS.gene90094.t1	AT3G63400	57.143	84	25	2	70	145	9	89	3.65e-18	80.9
MS.gene90094.t1	AT3G63400	57.143	84	25	2	70	145	9	89	3.92e-18	81.3
MS.gene90094.t1	AT3G63400	57.143	84	25	2	70	145	9	89	3.92e-18	81.3
MS.gene90094.t1	AT3G63400	57.143	84	25	2	70	145	9	89	3.92e-18	81.3
MS.gene90094.t1	AT2G38730	53.086	81	28	3	70	143	34	111	1.57e-17	76.6
MS.gene90094.t1	AT2G15790	58.904	73	19	2	69	133	5	74	1.72e-17	79.0
MS.gene90094.t1	AT3G56070	51.948	77	27	2	69	138	5	78	4.37e-17	75.1
MS.gene90094.t1	AT3G56070	51.948	77	27	2	69	138	5	78	4.37e-17	75.1
MS.gene90094.t1	AT4G34870	51.948	77	27	2	69	138	5	78	9.36e-17	73.9
MS.gene90094.t1	AT3G22920	50.000	72	26	2	69	133	5	73	7.98e-14	67.4

Find 50 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGAGGAGACTTTGATAA+AGG	0.345816	1.4:-88669350	MS.gene90094:CDS
TCCACCAGTGAGAAGGGCTT+TGG	0.372862	1.4:-88669419	MS.gene90094:CDS
ATCTATCTATCGCAGGGAAC+TGG	0.385423	1.4:-88669262	MS.gene90094:CDS
CTTCCAACCAGCTTCCCAAC+TGG	0.403156	1.4:+88669503	None:intergenic
AAAGTGAGGGCAGTGGAAAA+TGG	0.408575	1.4:-88669602	MS.gene90094:CDS
GCATCTCCGTCGGGGTGGTT+AGG	0.409454	1.4:-88669673	MS.gene90094:CDS
GGAAGTTCTCAACAGTTTGC+GGG	0.417261	1.4:+88669450	None:intergenic
GTATTCTTTGATGTTAGTAT+TGG	0.430298	1.4:-88669530	MS.gene90094:CDS
GGTGGTTAGGTCCCGAGGTG+TGG	0.434983	1.4:-88669660	MS.gene90094:CDS
AGGATTGTGATAGGACTCTT+TGG	0.436041	1.4:-88669482	MS.gene90094:CDS
GCCCTTCTCACTGGTGGAAA+GGG	0.436297	1.4:+88669424	None:intergenic
GTGGTTAGGTCCCGAGGTGT+GGG	0.453738	1.4:-88669659	MS.gene90094:CDS
TGGAAATCCAGTTGGGAAGC+TGG	0.456366	1.4:-88669510	MS.gene90094:CDS
TGCCCTTTCCACCAGTGAGA+AGG	0.468665	1.4:-88669426	MS.gene90094:CDS
CTAAAAGAAGCATCTCCGTC+GGG	0.471283	1.4:-88669682	MS.gene90094:CDS
ACTGGAGGCAAAAGTATCTC+TGG	0.489609	1.4:-88669244	MS.gene90094:CDS
AGCCCTTCTCACTGGTGGAA+AGG	0.493291	1.4:+88669423	None:intergenic
ATCAAGGACTTCATGATTCA+AGG	0.494593	1.4:-88669368	MS.gene90094:CDS
TCTATCTATCTATCTATCGC+AGG	0.505040	1.4:-88669269	MS.gene90094:CDS
GCCCTTTCCACCAGTGAGAA+GGG	0.506529	1.4:-88669425	MS.gene90094:CDS
CACCTTCCATCGCGTCATCA+AGG	0.515078	1.4:-88669384	MS.gene90094:CDS
GTTAGTATTGGAAATCCAGT+TGG	0.515418	1.4:-88669518	MS.gene90094:CDS
CGGAAGTTCTCAACAGTTTG+CGG	0.530427	1.4:+88669449	None:intergenic
TAGTTTCACACCCACACCTC+GGG	0.541866	1.4:+88669649	None:intergenic
GCCAAAGCCCTTCTCACTGG+TGG	0.549951	1.4:+88669418	None:intergenic
GTAGCCAAAGCCCTTCTCAC+TGG	0.550204	1.4:+88669415	None:intergenic
CAGTTGGGAAGCTGGTTGGA+AGG	0.554098	1.4:-88669502	MS.gene90094:CDS
TTAGTATTGGAAATCCAGTT+GGG	0.555401	1.4:-88669517	MS.gene90094:CDS
CTTGATGACGCGATGGAAGG+TGG	0.555450	1.4:+88669385	None:intergenic
CTGGTTGGAAGGATTGTGAT+AGG	0.560590	1.4:-88669491	MS.gene90094:CDS
AATCCAGTTGGGAAGCTGGT+TGG	0.562899	1.4:-88669506	MS.gene90094:CDS
GTCGGGGTGGTTAGGTCCCG+AGG	0.565995	1.4:-88669665	MS.gene90094:CDS
GGAAAATGGCAGTAGTTCAG+AGG	0.589618	1.4:-88669588	MS.gene90094:CDS
TTGAATGCAGCAACAGCCAT+CGG	0.590024	1.4:+88669867	None:intergenic
CTATCTATCTATCTATCGCA+GGG	0.594057	1.4:-88669268	MS.gene90094:CDS
TCTCACTGGTGGAAAGGGCA+CGG	0.601235	1.4:+88669429	None:intergenic
CCTAAAAGAAGCATCTCCGT+CGG	0.606730	1.4:-88669683	MS.gene90094:CDS
ACAAGTGAAAGTGAGGGCAG+TGG	0.614839	1.4:-88669609	MS.gene90094:CDS
CTAGTTTCACACCCACACCT+CGG	0.617486	1.4:+88669648	None:intergenic
TACGACGACAAGTGAAAGTG+AGG	0.618754	1.4:-88669616	MS.gene90094:CDS
GTCCTTGATGACGCGATGGA+AGG	0.624852	1.4:+88669382	None:intergenic
TGAAGTCCTTGATGACGCGA+TGG	0.626809	1.4:+88669378	None:intergenic
GATGACGCGATGGAAGGTGG+AGG	0.631465	1.4:+88669388	None:intergenic
GTGGGTGTGAAACTAGCAGT+AGG	0.631670	1.4:-88669641	MS.gene90094:CDS
TATCTATCGCAGGGAACTGG+AGG	0.635120	1.4:-88669259	MS.gene90094:CDS
TCGGGACCTAACCACCCCGA+CGG	0.658560	1.4:+88669667	None:intergenic
AAGAAGCATCTCCGTCGGGG+TGG	0.659605	1.4:-88669678	MS.gene90094:CDS
TAAAAGAAGCATCTCCGTCG+GGG	0.693386	1.4:-88669681	MS.gene90094:CDS
ACGACGACAAGTGAAAGTGA+GGG	0.708194	1.4:-88669615	MS.gene90094:CDS
AAGGACTTCATGATTCAAGG+AGG	0.745105	1.4:-88669365	MS.gene90094:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATCAATTAAATTTAAGTAA+TGG	+	chr1.4:88669306-88669325	None:intergenic	15.0%
!!!	ATAAGTTGATGAATAAAAAA+AGG	+	chr1.4:88669802-88669821	None:intergenic	15.0%
!!	TATTAATTGATTGATTGTCT+TGG	-	chr1.4:88669339-88669358	MS.gene90094:CDS	20.0%
!	GTATTCTTTGATGTTAGTAT+TGG	-	chr1.4:88669560-88669579	MS.gene90094:CDS	25.0%
!	TATATTATCAGTGAGAAAAG+TGG	+	chr1.4:88669265-88669284	None:intergenic	25.0%
!!	GTTGATGAATAAAAAAAGGA+AGG	+	chr1.4:88669798-88669817	None:intergenic	25.0%
	AATTGATTGATTGTCTTGGT+TGG	-	chr1.4:88669343-88669362	MS.gene90094:CDS	30.0%
	ATTGATTGATTGTCTTGGTT+GGG	-	chr1.4:88669344-88669363	MS.gene90094:CDS	30.0%
!	TTAGTATTGGAAATCCAGTT+GGG	-	chr1.4:88669573-88669592	MS.gene90094:CDS	30.0%
	ATCAAGGACTTCATGATTCA+AGG	-	chr1.4:88669722-88669741	MS.gene90094:intron	35.0%
	ATTATCAGTGAGAAAAGTGG+CGG	+	chr1.4:88669262-88669281	None:intergenic	35.0%
	CTATCTATCTATCTATCGCA+GGG	-	chr1.4:88669822-88669841	MS.gene90094:intron	35.0%
	TCTATCTATCTATCTATCGC+AGG	-	chr1.4:88669821-88669840	MS.gene90094:intron	35.0%
!	GTTAGTATTGGAAATCCAGT+TGG	-	chr1.4:88669572-88669591	MS.gene90094:CDS	35.0%
!!!	CTTCTTTTAGGGTTAGGATT+AGG	+	chr1.4:88669398-88669417	None:intergenic	35.0%
	AAGGACTTCATGATTCAAGG+AGG	-	chr1.4:88669725-88669744	MS.gene90094:intron	40.0%
	AGGATTGTGATAGGACTCTT+TGG	-	chr1.4:88669608-88669627	MS.gene90094:CDS	40.0%
	CAAGGAGGAGACTTTGATAA+AGG	-	chr1.4:88669740-88669759	MS.gene90094:intron	40.0%
!!!	GAGATGCTTCTTTTAGGGTT+AGG	+	chr1.4:88669404-88669423	None:intergenic	40.0%
	AAAGTGAGGGCAGTGGAAAA+TGG	-	chr1.4:88669488-88669507	MS.gene90094:CDS	45.0%
	ACGACGACAAGTGAAAGTGA+GGG	-	chr1.4:88669475-88669494	MS.gene90094:CDS	45.0%
	ATCTATCTATCGCAGGGAAC+TGG	-	chr1.4:88669828-88669847	MS.gene90094:intron	45.0%
	CGGAAGTTCTCAACAGTTTG+CGG	+	chr1.4:88669644-88669663	None:intergenic	45.0%
	CTGGTTGGAAGGATTGTGAT+AGG	-	chr1.4:88669599-88669618	MS.gene90094:CDS	45.0%
	GATTGATTGTCTTGGTTGGG+TGG	-	chr1.4:88669347-88669366	MS.gene90094:CDS	45.0%
	GGAAAATGGCAGTAGTTCAG+AGG	-	chr1.4:88669502-88669521	MS.gene90094:CDS	45.0%
	GGAAGTTCTCAACAGTTTGC+GGG	+	chr1.4:88669643-88669662	None:intergenic	45.0%
	TACGACGACAAGTGAAAGTG+AGG	-	chr1.4:88669474-88669493	MS.gene90094:CDS	45.0%
!	CGACGGAGATGCTTCTTTTA+GGG	+	chr1.4:88669409-88669428	None:intergenic	45.0%
!!	ACTGGAGGCAAAAGTATCTC+TGG	-	chr1.4:88669846-88669865	MS.gene90094:intron	45.0%
!!	CCTAAAAGAAGCATCTCCGT+CGG	-	chr1.4:88669407-88669426	MS.gene90094:CDS	45.0%
!!	CTAAAAGAAGCATCTCCGTC+GGG	-	chr1.4:88669408-88669427	MS.gene90094:CDS	45.0%
!!	TAAAAGAAGCATCTCCGTCG+GGG	-	chr1.4:88669409-88669428	MS.gene90094:CDS	45.0%
	AATCCAGTTGGGAAGCTGGT+TGG	-	chr1.4:88669584-88669603	MS.gene90094:CDS	50.0%
	ACAAGTGAAAGTGAGGGCAG+TGG	-	chr1.4:88669481-88669500	MS.gene90094:CDS	50.0%
	CTAGTTTCACACCCACACCT+CGG	+	chr1.4:88669445-88669464	None:intergenic	50.0%
	GTGGGTGTGAAACTAGCAGT+AGG	-	chr1.4:88669449-88669468	MS.gene90094:CDS	50.0%
	TAGTTTCACACCCACACCTC+GGG	+	chr1.4:88669444-88669463	None:intergenic	50.0%
	TATCTATCGCAGGGAACTGG+AGG	-	chr1.4:88669831-88669850	MS.gene90094:intron	50.0%
	TGGAAATCCAGTTGGGAAGC+TGG	-	chr1.4:88669580-88669599	MS.gene90094:CDS	50.0%
!	CCGACGGAGATGCTTCTTTT+AGG	+	chr1.4:88669410-88669429	None:intergenic	50.0%
!!	TGAAGTCCTTGATGACGCGA+TGG	+	chr1.4:88669715-88669734	None:intergenic	50.0%
	AGCCCTTCTCACTGGTGGAA+AGG	+	chr1.4:88669670-88669689	None:intergenic	55.0%
	CACCTTCCATCGCGTCATCA+AGG	-	chr1.4:88669706-88669725	MS.gene90094:CDS	55.0%
	CAGTTGGGAAGCTGGTTGGA+AGG	-	chr1.4:88669588-88669607	MS.gene90094:CDS	55.0%
	CTTCCAACCAGCTTCCCAAC+TGG	+	chr1.4:88669590-88669609	None:intergenic	55.0%
	GCCCTTCTCACTGGTGGAAA+GGG	+	chr1.4:88669669-88669688	None:intergenic	55.0%
	GCCCTTTCCACCAGTGAGAA+GGG	-	chr1.4:88669665-88669684	MS.gene90094:CDS	55.0%
	GTAGCCAAAGCCCTTCTCAC+TGG	+	chr1.4:88669678-88669697	None:intergenic	55.0%
	TCCACCAGTGAGAAGGGCTT+TGG	-	chr1.4:88669671-88669690	MS.gene90094:CDS	55.0%
	TCTCACTGGTGGAAAGGGCA+CGG	+	chr1.4:88669664-88669683	None:intergenic	55.0%
	TGCCCTTTCCACCAGTGAGA+AGG	-	chr1.4:88669664-88669683	MS.gene90094:CDS	55.0%
!!	CTTGATGACGCGATGGAAGG+TGG	+	chr1.4:88669708-88669727	None:intergenic	55.0%
!!	GTCCTTGATGACGCGATGGA+AGG	+	chr1.4:88669711-88669730	None:intergenic	55.0%
	GATGACGCGATGGAAGGTGG+AGG	+	chr1.4:88669705-88669724	None:intergenic	60.0%
	GCCAAAGCCCTTCTCACTGG+TGG	+	chr1.4:88669675-88669694	None:intergenic	60.0%
	GTGGTTAGGTCCCGAGGTGT+GGG	-	chr1.4:88669431-88669450	MS.gene90094:CDS	60.0%
!!	AAGAAGCATCTCCGTCGGGG+TGG	-	chr1.4:88669412-88669431	MS.gene90094:CDS	60.0%
	GCATCTCCGTCGGGGTGGTT+AGG	-	chr1.4:88669417-88669436	MS.gene90094:CDS	65.0%
	GGTGGTTAGGTCCCGAGGTG+TGG	-	chr1.4:88669430-88669449	MS.gene90094:CDS	65.0%
	TCGGGACCTAACCACCCCGA+CGG	+	chr1.4:88669426-88669445	None:intergenic	65.0%
	GTCGGGGTGGTTAGGTCCCG+AGG	-	chr1.4:88669425-88669444	MS.gene90094:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	88669226	88669886	88669226	ID=MS.gene90094
chr1.4	mRNA	88669226	88669886	88669226	ID=MS.gene90094.t1;Parent=MS.gene90094
chr1.4	exon	88669866	88669886	88669866	ID=MS.gene90094.t1.exon1;Parent=MS.gene90094.t1
chr1.4	CDS	88669866	88669886	88669866	ID=cds.MS.gene90094.t1;Parent=MS.gene90094.t1
chr1.4	exon	88669226	88669738	88669226	ID=MS.gene90094.t1.exon2;Parent=MS.gene90094.t1
chr1.4	CDS	88669226	88669738	88669226	ID=cds.MS.gene90094.t1;Parent=MS.gene90094.t1

Gene Sequence

>MS.gene90094

ATGGCTGTTGCTGCATTCAATGTTACTACAACACCGCCACTTTTCTCACTGATAATATATATATATATATATATTTTCCATTACTTAAATTTAATTGATGTTGAATTCATTTTTATTAATTGATTGATTGTCTTGGTTGGGTGGTCAGTCAAATGTTGTTGCTCTGAATCCTAATCCTAACCCTAAAAGAAGCATCTCCGTCGGGGTGGTTAGGTCCCGAGGTGTGGGTGTGAAACTAGCAGTAGGAGTACGACGACAAGTGAAAGTGAGGGCAGTGGAAAATGGCAGTAGTTCAGAGGAGAAAGTAATAGTACAGTCAAAAGTGACTCACAAAGTATTCTTTGATGTTAGTATTGGAAATCCAGTTGGGAAGCTGGTTGGAAGGATTGTGATAGGACTCTTTGGTGATGATGTCCCGCAAACTGTTGAGAACTTCCGTGCCCTTTCCACCAGTGAGAAGGGCTTTGGCTACAAGTCCTCCACCTTCCATCGCGTCATCAAGGACTTCATGATTCAAGGAGGAGACTTTGATAAAGGAAATGTATGTATCTCTCTATCTATCGCTATCTCCTTCCTTTTTTTATTCATCAACTTATCTATCTATCTATCTATCGCAGGGAACTGGAGGCAAAAGTATCTCTGGTCGTACTTTCAAAGATGA

Protein sequence

>MS.gene90094.t1

MAVAAFNSNVVALNPNPNPKRSISVGVVRSRGVGVKLAVGVRRQVKVRAVENGSSSEEKVIVQSKVTHKVFFDVSIGNPVGKLVGRIVIGLFGDDVPQTVENFRALSTSEKGFGYKSSTFHRVIKDFMIQGGDFDKGNVCISLSIAISFLFLFINLSIYLSIAGNWRQKYLWSYFQR