AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90168

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90168.t1 MTR_5g029990 98.322 298 5 0 1 298 1 298 0.0 619
MS.gene90168.t1 MTR_6g406250 31.373 306 186 8 6 293 49 348 1.32e-49 168
MS.gene90168.t1 MTR_2g104960 31.803 305 189 9 4 296 12 309 2.81e-38 138
MS.gene90168.t1 MTR_3g005210 29.316 307 193 9 7 296 1 300 1.26e-36 133
MS.gene90168.t1 MTR_5g072620 30.719 306 186 8 9 296 2 299 4.63e-36 132
MS.gene90168.t1 MTR_3g005170 28.053 303 196 7 10 296 8 304 4.27e-35 129
MS.gene90168.t1 MTR_4g009040 31.494 308 171 10 12 296 13 303 5.20e-35 129
MS.gene90168.t1 MTR_4g009040 30.769 312 172 10 12 296 13 307 1.42e-34 128
MS.gene90168.t1 MTR_3g005170 28.881 277 176 6 10 271 8 278 2.84e-34 126
MS.gene90168.t1 MTR_4g006940 31.731 312 188 10 3 296 2 306 3.38e-34 127
MS.gene90168.t1 MTR_8g062440 31.373 306 179 10 11 296 7 301 1.20e-33 125
MS.gene90168.t1 MTR_8g062440 30.592 304 187 9 10 296 6 302 2.35e-33 125
MS.gene90168.t1 MTR_8g062110 29.097 299 193 6 12 296 9 302 1.13e-32 123
MS.gene90168.t1 MTR_8g062110 29.097 299 193 6 12 296 18 311 1.18e-32 123
MS.gene90168.t1 MTR_2g101330 30.827 266 168 5 10 260 3 267 1.33e-32 123
MS.gene90168.t1 MTR_3g031650 29.316 307 189 9 9 296 2 299 1.38e-32 122
MS.gene90168.t1 MTR_8g062440 31.707 287 172 8 11 280 7 286 1.45e-32 122
MS.gene90168.t1 MTR_1g022445 30.063 316 179 11 9 297 5 305 3.26e-32 122
MS.gene90168.t1 MTR_4g006940 33.591 259 148 9 3 243 2 254 1.15e-31 119
MS.gene90168.t1 MTR_4g006940 33.591 259 148 9 3 243 2 254 1.15e-31 119
MS.gene90168.t1 MTR_1g022440 30.576 278 160 10 9 263 5 272 1.46e-31 120
MS.gene90168.t1 MTR_3g031650 30.037 273 166 7 9 263 2 267 2.42e-31 118
MS.gene90168.t1 MTR_8g062110 29.104 268 172 5 11 264 8 271 2.67e-31 118
MS.gene90168.t1 MTR_8g062110 29.104 268 172 5 11 264 8 271 3.67e-31 118
MS.gene90168.t1 MTR_6g406250 31.455 213 134 5 6 206 49 261 1.14e-30 116
MS.gene90168.t1 MTR_4g077130 28.013 307 187 10 12 296 9 303 6.26e-30 115
MS.gene90168.t1 MTR_5g056600 27.236 246 161 6 13 243 5 247 6.61e-30 115
MS.gene90168.t1 MTR_4g077100 27.483 302 195 8 12 296 11 305 7.50e-30 115
MS.gene90168.t1 MTR_4g077100 28.458 253 161 5 12 248 11 259 9.89e-30 114
MS.gene90168.t1 MTR_3g005170 28.188 298 171 9 10 296 8 273 7.09e-28 109
MS.gene90168.t1 MTR_4g009690 31.660 259 151 6 12 252 13 263 1.64e-27 109
MS.gene90168.t1 MTR_4g009690 31.154 260 152 6 12 252 13 264 2.84e-27 108
MS.gene90168.t1 MTR_7g074880 28.625 269 166 7 12 260 8 270 3.60e-27 108
MS.gene90168.t1 MTR_7g074850 28.777 278 162 10 12 263 8 275 3.95e-25 102
MS.gene90168.t1 MTR_7g074820 29.278 263 159 9 12 252 8 265 6.36e-25 102
MS.gene90168.t1 MTR_7g074730 27.675 271 173 8 12 263 8 274 9.59e-25 102
MS.gene90168.t1 MTR_4g081440 28.435 313 183 13 13 292 25 329 4.44e-24 100
MS.gene90168.t1 MTR_7g074710 28.253 269 167 6 12 260 8 270 6.06e-24 99.8
MS.gene90168.t1 MTR_2g028620 29.961 257 152 9 12 247 10 259 1.99e-23 98.2
MS.gene90168.t1 MTR_4g081440 27.673 318 188 13 13 297 21 329 2.09e-23 98.6
MS.gene90168.t1 MTR_2g028620 29.961 257 152 9 12 247 10 259 4.04e-23 97.1
MS.gene90168.t1 MTR_8g062110 29.327 208 133 4 98 296 14 216 1.64e-20 88.6
MS.gene90168.t1 MTR_4g023730 32.022 178 106 5 11 177 7 180 4.27e-20 89.0
MS.gene90168.t1 MTR_8g062110 28.889 180 115 3 94 264 10 185 4.08e-19 83.6
MS.gene90168.t1 MTR_7g074870 26.667 225 150 4 48 260 11 232 1.99e-18 84.0
MS.gene90168.t1 MTR_4g092080 25.468 267 171 8 5 246 7 270 8.02e-18 82.8
MS.gene90168.t1 MTR_2g013250 23.188 276 177 7 12 254 7 280 6.19e-15 74.3
MS.gene90168.t1 MTR_2g013230 25.882 170 116 5 12 171 8 177 9.15e-12 65.1
MS.gene90168.t1 MTR_7g117190 26.351 148 103 3 12 153 7 154 9.65e-12 64.7
MS.gene90168.t1 MTR_7g117190 26.351 148 103 3 12 153 7 154 1.19e-11 64.7
MS.gene90168.t1 MTR_2g013260 24.706 170 118 4 12 171 8 177 1.93e-11 63.9
MS.gene90168.t1 MTR_2g013260 24.706 170 118 4 12 171 8 177 2.77e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90168.t1 AT2G23910 44.816 299 146 7 8 293 6 298 8.60e-87 262
MS.gene90168.t1 AT4G30470 43.667 300 151 6 6 293 4 297 4.27e-84 255
MS.gene90168.t1 AT2G23910 46.617 266 128 5 8 263 6 267 1.22e-82 250
MS.gene90168.t1 AT2G23910 46.617 266 128 5 8 263 6 267 1.40e-82 250
MS.gene90168.t1 AT2G23910 44.574 258 128 6 45 293 13 264 1.78e-73 227
MS.gene90168.t1 AT5G14700 30.323 310 193 8 6 293 49 357 7.76e-41 145
MS.gene90168.t1 AT5G58490 30.921 304 184 7 11 296 8 303 8.00e-38 136
MS.gene90168.t1 AT1G51410 31.023 303 185 8 11 296 8 303 7.51e-37 134
MS.gene90168.t1 AT1G09510 32.171 258 153 7 12 252 8 260 7.31e-35 129
MS.gene90168.t1 AT2G02400 30.769 299 177 10 7 281 1 293 9.31e-35 128
MS.gene90168.t1 AT4G35420 28.013 307 190 7 5 297 1 290 4.36e-34 127
MS.gene90168.t1 AT5G19440 29.470 302 189 8 12 296 10 304 5.84e-34 126
MS.gene90168.t1 AT1G15950 30.537 298 188 8 11 296 12 302 2.24e-32 122
MS.gene90168.t1 AT1G15950 30.537 298 188 8 11 296 12 302 2.40e-32 122
MS.gene90168.t1 AT1G15950 30.537 298 188 8 11 296 12 302 2.40e-32 122
MS.gene90168.t1 AT1G09480 28.852 305 187 8 12 296 8 302 2.44e-32 122
MS.gene90168.t1 AT1G80820 30.921 304 179 9 12 297 8 298 2.59e-32 122
MS.gene90168.t1 AT1G09480 28.852 305 187 8 12 296 55 349 5.06e-32 122
MS.gene90168.t1 AT1G15950 31.959 291 176 10 11 289 12 292 1.44e-31 119
MS.gene90168.t1 AT1G80820 34.137 249 134 8 12 242 8 244 3.61e-31 117
MS.gene90168.t1 AT2G33600 30.945 307 177 8 7 292 4 296 6.58e-31 118
MS.gene90168.t1 AT1G15950 32.075 265 161 8 11 263 12 269 7.62e-31 117
MS.gene90168.t1 AT1G15950 32.075 265 161 8 11 263 12 269 8.49e-31 117
MS.gene90168.t1 AT2G33590 30.097 309 179 9 6 292 3 296 9.76e-31 117
MS.gene90168.t1 AT1G09490 29.412 306 188 9 10 296 6 302 4.37e-30 116
MS.gene90168.t1 AT1G66800 30.855 269 159 7 12 263 8 266 4.98e-29 113
MS.gene90168.t1 AT1G68540 27.642 246 160 6 14 243 6 249 6.24e-28 110
MS.gene90168.t1 AT5G42800 31.923 260 145 9 10 246 6 256 6.95e-28 111
MS.gene90168.t1 AT4G27250 26.045 311 200 9 11 297 12 316 1.31e-25 104
MS.gene90168.t1 AT1G25460 27.519 258 163 9 25 263 17 269 1.77e-25 103
MS.gene90168.t1 AT4G27250 32.632 190 115 5 11 187 12 201 2.69e-25 103
MS.gene90168.t1 AT1G09500 28.405 257 165 5 12 252 8 261 1.67e-24 100
MS.gene90168.t1 AT1G09500 28.405 257 165 5 12 252 8 261 3.77e-24 100
MS.gene90168.t1 AT5G14700 29.493 217 137 5 6 206 49 265 8.67e-24 98.6
MS.gene90168.t1 AT1G61720 27.481 262 158 7 9 243 10 266 3.01e-22 95.1
MS.gene90168.t1 AT1G09490 30.088 226 135 7 56 266 25 242 4.10e-22 94.0
MS.gene90168.t1 AT1G76470 27.687 307 193 10 10 296 5 302 1.01e-20 90.9
MS.gene90168.t1 AT2G45400 26.048 334 188 11 3 297 31 344 7.07e-19 86.3
MS.gene90168.t1 AT2G45400 26.909 275 156 7 2 243 30 292 2.47e-18 84.0
MS.gene90168.t1 AT2G45400 28.972 214 136 4 3 202 31 242 6.08e-18 82.0
MS.gene90168.t1 AT1G68540 27.632 152 100 3 110 252 1 151 1.10e-17 80.5
MS.gene90168.t1 AT1G09500 27.273 209 137 4 56 252 22 227 5.82e-16 77.0
MS.gene90168.t1 AT1G15950 28.986 207 136 5 98 296 1 204 3.77e-15 73.9

Find 58 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAAGTTGAACAAATTAA+TGG 0.319570 5.1:+20564051 MS.gene90168:CDS
GTCTTTGTGACCGTTGATTT+AGG 0.322077 5.1:+20563668 MS.gene90168:CDS
AGCATAATTGATGCTCTTAG+AGG 0.347208 5.1:+20561519 MS.gene90168:CDS
GTTTGTGTTATGGATGCTTC+AGG 0.359431 5.1:+20561168 MS.gene90168:CDS
TAAGACCATAGAACCTGATT+TGG 0.363147 5.1:+20562848 MS.gene90168:CDS
CATGCAACCATTCAACAATA+TGG 0.369425 5.1:+20561243 MS.gene90168:CDS
TGATAATGAACAGGAATATA+TGG 0.371532 5.1:+20562710 MS.gene90168:intron
TTCATCCAAATCAGGTTCTA+TGG 0.374831 5.1:-20562853 None:intergenic
CAATGTATTTCATCCAAATC+AGG 0.379342 5.1:-20562861 None:intergenic
TAGTCATACTGTTTGTGTTA+TGG 0.382831 5.1:+20561158 MS.gene90168:CDS
ATGGATGCTTCAGGTCAATT+AGG 0.386860 5.1:+20561177 MS.gene90168:CDS
CAAATTAATGGTGGACTTTG+AGG 0.389339 5.1:+20564063 MS.gene90168:CDS
CTTCAGCAACTGCTGTCGTT+TGG 0.399258 5.1:+20562814 MS.gene90168:CDS
AATGGACAGAGGAGTAAATA+TGG 0.399954 5.1:+20563556 MS.gene90168:CDS
TCAGCAGCTCCTCTCAAGTA+AGG 0.433540 5.1:-20563629 None:intergenic
GATGCTCTTAGAGGTTGCTC+TGG 0.436699 5.1:+20561528 MS.gene90168:CDS
ATTATGCTATGATAATCAAA+AGG 0.437929 5.1:-20561504 None:intergenic
TCATAATTGGGCTGGTCAAA+GGG 0.439042 5.1:-20561573 None:intergenic
ATCATAATTGGGCTGGTCAA+AGG 0.444436 5.1:-20561574 None:intergenic
TGCTTACATCATAATTGGGC+TGG 0.462300 5.1:-20561581 None:intergenic
GGTGTTTGATGGAGAGATCA+TGG 0.467351 5.1:-20563607 None:intergenic
CAGCAACTGCTGTCGTTTGG+AGG 0.468466 5.1:+20562817 MS.gene90168:CDS
TCTCAAGTAAGGGTGTTTGA+TGG 0.471366 5.1:-20563618 None:intergenic
CAATTTCTGTCGCAAATTCA+AGG 0.478165 5.1:+20562893 MS.gene90168:CDS
ATGTTGTGCAGTGATGACTA+TGG 0.478181 5.1:+20563998 MS.gene90168:intron
GCAGGAGAAGATCCATTCAA+TGG 0.492642 5.1:+20561438 MS.gene90168:intron
TCAGCTGAAATCCCATTGAA+TGG 0.493043 5.1:-20561450 None:intergenic
TACTCCTCTGTCCATTGCTA+AGG 0.497399 5.1:-20563549 None:intergenic
ATAATTCAGTCATACCTTTG+TGG 0.497881 5.1:-20563833 None:intergenic
TCAGTCATACCTTTGTGGTA+AGG 0.505180 5.1:-20563828 None:intergenic
GTTCAAAGACTTCTTCAAAG+AGG 0.517969 5.1:+20561210 MS.gene90168:CDS
AGCAACTGCTGTCGTTTGGA+GGG 0.521728 5.1:+20562818 MS.gene90168:CDS
CAGGAGAAGATCCATTCAAT+GGG 0.530786 5.1:+20561439 MS.gene90168:intron
TTATGCTATGATAATCAAAA+GGG 0.531153 5.1:-20561503 None:intergenic
ATATGTACCATATTGTTGAA+TGG 0.532190 5.1:-20561250 None:intergenic
TGACTAGTTATGTCAAAAGA+AGG 0.541260 5.1:-20561141 None:intergenic
CTGATTTGGATGAAATACAT+TGG 0.555033 5.1:+20562862 MS.gene90168:CDS
ACAAACAAACAGTTATGGCA+TGG 0.567066 5.1:+20563491 MS.gene90168:intron
AATATGGTATCAATCAATGC+AGG 0.575934 5.1:+20563572 MS.gene90168:CDS
TTGTTGAATGGTTGCATGAA+CGG 0.577578 5.1:-20561238 None:intergenic
TACGAGGATGTCTCATCGTA+CGG 0.580649 5.1:+20563719 MS.gene90168:CDS
AACAGCGTTTGCCTTAGCAA+TGG 0.587609 5.1:+20563538 MS.gene90168:CDS
CAGCAGCTCCTCTCAAGTAA+GGG 0.598914 5.1:-20563628 None:intergenic
ATCAAACACCCTTACTTGAG+AGG 0.605366 5.1:+20563620 MS.gene90168:CDS
TTTGAAGAAGTCTTTGAACA+AGG 0.606201 5.1:-20561206 None:intergenic
CAAACAAACAGTTATGGCAT+GGG 0.606327 5.1:+20563492 MS.gene90168:intron
GTGTTTGATGGAGAGATCAT+GGG 0.613888 5.1:-20563606 None:intergenic
GAAGTTGAACAAATTAATGG+TGG 0.616912 5.1:+20564054 MS.gene90168:CDS
TAACTTATGTGCAAGCTGAA+CGG 0.619871 5.1:-20563786 None:intergenic
TTGTGGTAAGGAAGAAGATG+TGG 0.620890 5.1:-20563816 None:intergenic
CATCTTCTTCCTTACCACAA+AGG 0.639609 5.1:+20563819 MS.gene90168:CDS
TTTGCCTTAGCAATGGACAG+AGG 0.681382 5.1:+20563545 MS.gene90168:CDS
TACTCACATCTGTGTCTACG+AGG 0.683844 5.1:+20563703 MS.gene90168:CDS
GCTGCTGAAATGTACGAGGA+TGG 0.684788 5.1:+20563644 MS.gene90168:CDS
ACAGGAATATATGGCTGACG+TGG 0.685182 5.1:+20562719 MS.gene90168:intron
TAATTGGGCTGGTCAAAGGG+AGG 0.685897 5.1:-20561570 None:intergenic
GGTAAGGAAGAAGATGTGGT+AGG 0.716806 5.1:-20563812 None:intergenic
AGGAGCTGCTGAAATGTACG+AGG 0.764112 5.1:+20563640 MS.gene90168:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGATTAAAAAGTTAAAA+AGG - chr5.1:20561620-20561639 None:intergenic 10.0%
!! AAAGATTAAAAAGTTAAAAA+GGG - chr5.1:20561619-20561638 None:intergenic 10.0%
!! TAAAAAAATTCATAGAAATT+TGG + chr5.1:20561780-20561799 MS.gene90168:intron 10.0%
!! TATAATTTCAATCATTAAAT+TGG + chr5.1:20562974-20562993 MS.gene90168:intron 10.0%
!! AAAATTTGTAACATAAACAT+TGG - chr5.1:20561749-20561768 None:intergenic 15.0%
!!! ATATGTTAAAGTTTGAAATT+TGG + chr5.1:20563124-20563143 MS.gene90168:intron 15.0%
!!! ATGATTGAAATTATAAGTTT+TGG - chr5.1:20562969-20562988 None:intergenic 15.0%
!!! TCAAACATTTTAACTTATAA+AGG + chr5.1:20563860-20563879 MS.gene90168:intron 15.0%
!!! TTTATTTGATTTTGACTATA+TGG + chr5.1:20563944-20563963 MS.gene90168:intron 15.0%
!! AAAAACAAATACTTCTACAT+GGG - chr5.1:20562607-20562626 None:intergenic 20.0%
!! AAGCACTAATACAAATTTAA+AGG - chr5.1:20561957-20561976 None:intergenic 20.0%
!! ATTATGCTATGATAATCAAA+AGG - chr5.1:20561507-20561526 None:intergenic 20.0%
!! ATTCATCTTAAATGAATAAG+TGG + chr5.1:20561304-20561323 MS.gene90168:intron 20.0%
!! CTAAGCAAATTATTGTAATT+TGG + chr5.1:20562117-20562136 MS.gene90168:intron 20.0%
!! GAAAAAATGATTTAGATTGT+GGG + chr5.1:20563036-20563055 MS.gene90168:intron 20.0%
!! TAAAAACAAATACTTCTACA+TGG - chr5.1:20562608-20562627 None:intergenic 20.0%
!! TATACTTTAAACATTGTGTT+TGG + chr5.1:20562929-20562948 MS.gene90168:intron 20.0%
!! TGAAAAAATGATTTAGATTG+TGG + chr5.1:20563035-20563054 MS.gene90168:intron 20.0%
!! TTATGCTATGATAATCAAAA+GGG - chr5.1:20561506-20561525 None:intergenic 20.0%
!!! ATTCAAATAAGGTTGTTTTA+TGG + chr5.1:20563884-20563903 MS.gene90168:intron 20.0%
!!! ATTCATAGAAATTTGGATTT+TGG + chr5.1:20561787-20561806 MS.gene90168:intron 20.0%
!!! CTTATAAAGGTATTCAAATA+AGG + chr5.1:20563873-20563892 MS.gene90168:intron 20.0%
!!! TTTTTGCTTACATCATAATT+GGG - chr5.1:20561588-20561607 None:intergenic 20.0%
! ATATGTACCATATTGTTGAA+TGG - chr5.1:20561253-20561272 None:intergenic 25.0%
! ATTGTACAAACAAACAGTTA+TGG + chr5.1:20563486-20563505 MS.gene90168:intron 25.0%
! CAAGAAGTTGAACAAATTAA+TGG + chr5.1:20564051-20564070 MS.gene90168:CDS 25.0%
! GATTTAAACAAAAATGTGAC+TGG - chr5.1:20563102-20563121 None:intergenic 25.0%
! TCAAATGACTAAGACATATT+TGG - chr5.1:20562204-20562223 None:intergenic 25.0%
!! AAAGGTATGACTGAATTATA+TGG + chr5.1:20563837-20563856 MS.gene90168:intron 25.0%
!! GTTTTTGCTTACATCATAAT+TGG - chr5.1:20561589-20561608 None:intergenic 25.0%
!! TGATAATGAACAGGAATATA+TGG + chr5.1:20562710-20562729 MS.gene90168:intron 25.0%
!!! AATAATTTGCTTAGTTGTCA+TGG - chr5.1:20562111-20562130 None:intergenic 25.0%
!!! AATTATAAGTTTTGGACAGT+AGG - chr5.1:20562961-20562980 None:intergenic 25.0%
!!! GACTCATTCATTTAGTTTTA+AGG + chr5.1:20563917-20563936 MS.gene90168:intron 25.0%
AATATGGTATCAATCAATGC+AGG + chr5.1:20563572-20563591 MS.gene90168:CDS 30.0%
AGTACTAGTATTCATCTTCT+TGG + chr5.1:20561811-20561830 MS.gene90168:intron 30.0%
ATAATTCAGTCATACCTTTG+TGG - chr5.1:20563836-20563855 None:intergenic 30.0%
ATCAATTGAGCTAAGTTCAT+TGG + chr5.1:20561374-20561393 MS.gene90168:intron 30.0%
CAATGTATTTCATCCAAATC+AGG - chr5.1:20562864-20562883 None:intergenic 30.0%
CACCAAAAATCCTAAATCAA+CGG - chr5.1:20563681-20563700 None:intergenic 30.0%
GAAGTTGAACAAATTAATGG+TGG + chr5.1:20564054-20564073 MS.gene90168:CDS 30.0%
TAGTCATACTGTTTGTGTTA+TGG + chr5.1:20561158-20561177 MS.gene90168:CDS 30.0%
TCAATTGAGCTAAGTTCATT+GGG + chr5.1:20561375-20561394 MS.gene90168:intron 30.0%
TCAGAATCATCTTATCTGAT+CGG - chr5.1:20563224-20563243 None:intergenic 30.0%
TGATTCTAGATAGACTCTTA+GGG - chr5.1:20561724-20561743 None:intergenic 30.0%
TTAGAATCATCTGAACTGAT+CGG - chr5.1:20563344-20563363 None:intergenic 30.0%
TTGATAAGATGATTCTGATG+TGG + chr5.1:20563280-20563299 MS.gene90168:intron 30.0%
TTGATTCTAGATAGACTCTT+AGG - chr5.1:20561725-20561744 None:intergenic 30.0%
TTGTTAACGATCAATCATGA+TGG + chr5.1:20562329-20562348 MS.gene90168:intron 30.0%
TTTGAAGAAGTCTTTGAACA+AGG - chr5.1:20561209-20561228 None:intergenic 30.0%
! TGACTAGTTATGTCAAAAGA+AGG - chr5.1:20561144-20561163 None:intergenic 30.0%
!! AACATGTCTTGATAATGAAC+AGG + chr5.1:20562701-20562720 MS.gene90168:intron 30.0%
!! CTGATTTGGATGAAATACAT+TGG + chr5.1:20562862-20562881 MS.gene90168:CDS 30.0%
!! TTGATCAGATGATTTTGATG+TGG + chr5.1:20563160-20563179 MS.gene90168:intron 30.0%
AAGTGAGAAACTCAGGTAAA+TGG - chr5.1:20562032-20562051 None:intergenic 35.0%
AATGGACAGAGGAGTAAATA+TGG + chr5.1:20563556-20563575 MS.gene90168:CDS 35.0%
ACAAACAAACAGTTATGGCA+TGG + chr5.1:20563491-20563510 MS.gene90168:intron 35.0%
AGCAAGCAAACACCATTTAT+TGG + chr5.1:20561991-20562010 MS.gene90168:intron 35.0%
AGCATAATTGATGCTCTTAG+AGG + chr5.1:20561519-20561538 MS.gene90168:CDS 35.0%
CAAACAAACAGTTATGGCAT+GGG + chr5.1:20563492-20563511 MS.gene90168:intron 35.0%
CAATTTCTGTCGCAAATTCA+AGG + chr5.1:20562893-20562912 MS.gene90168:CDS 35.0%
CAGATAAGATGATTCTGATG+TGG + chr5.1:20563226-20563245 MS.gene90168:intron 35.0%
CAGTTCAGATGATTCTAATG+TGG + chr5.1:20563346-20563365 MS.gene90168:intron 35.0%
CATGCAACCATTCAACAATA+TGG + chr5.1:20561243-20561262 MS.gene90168:CDS 35.0%
GACTAAGACATATTTGGTCA+AGG - chr5.1:20562198-20562217 None:intergenic 35.0%
GTTCAAAGACTTCTTCAAAG+AGG + chr5.1:20561210-20561229 MS.gene90168:CDS 35.0%
TAACTTATGTGCAAGCTGAA+CGG - chr5.1:20563789-20563808 None:intergenic 35.0%
TAAGACCATAGAACCTGATT+TGG + chr5.1:20562848-20562867 MS.gene90168:CDS 35.0%
TATGTCTTAGTCATTTGAGC+TGG + chr5.1:20562206-20562225 MS.gene90168:intron 35.0%
TTCATCCAAATCAGGTTCTA+TGG - chr5.1:20562856-20562875 None:intergenic 35.0%
TTGTTGAATGGTTGCATGAA+CGG - chr5.1:20561241-20561260 None:intergenic 35.0%
TTTGATATTTGCACGCAATG+TGG + chr5.1:20562356-20562375 MS.gene90168:intron 35.0%
! GAATTCGAGATTTGAAACTC+TGG + chr5.1:20561328-20561347 MS.gene90168:intron 35.0%
!! CAAATTAATGGTGGACTTTG+AGG + chr5.1:20564063-20564082 MS.gene90168:CDS 35.0%
!!! CGTTGATTTAGGATTTTTGG+TGG + chr5.1:20563679-20563698 MS.gene90168:CDS 35.0%
!!! GACCGTTGATTTAGGATTTT+TGG + chr5.1:20563676-20563695 MS.gene90168:CDS 35.0%
AACAAGCAAGTGAGAAACTC+AGG - chr5.1:20562039-20562058 None:intergenic 40.0%
ATCAAACACCCTTACTTGAG+AGG + chr5.1:20563620-20563639 MS.gene90168:CDS 40.0%
ATGGATGCTTCAGGTCAATT+AGG + chr5.1:20561177-20561196 MS.gene90168:CDS 40.0%
ATGTTGTGCAGTGATGACTA+TGG + chr5.1:20563998-20564017 MS.gene90168:intron 40.0%
CAATCCCAGAAGTAGAAAGA+GGG - chr5.1:20562062-20562081 None:intergenic 40.0%
CAGATGATTCTAATGTGGTG+TGG + chr5.1:20563351-20563370 MS.gene90168:intron 40.0%
CAGGAGAAGATCCATTCAAT+GGG + chr5.1:20561439-20561458 MS.gene90168:intron 40.0%
CATCTTCTTCCTTACCACAA+AGG + chr5.1:20563819-20563838 MS.gene90168:CDS 40.0%
GTGTTTGATGGAGAGATCAT+GGG - chr5.1:20563609-20563628 None:intergenic 40.0%
GTTTGTGTTATGGATGCTTC+AGG + chr5.1:20561168-20561187 MS.gene90168:CDS 40.0%
TCAATCCCAGAAGTAGAAAG+AGG - chr5.1:20562063-20562082 None:intergenic 40.0%
TCAGCTGAAATCCCATTGAA+TGG - chr5.1:20561453-20561472 None:intergenic 40.0%
TCAGTCATACCTTTGTGGTA+AGG - chr5.1:20563831-20563850 None:intergenic 40.0%
TGCTTACATCATAATTGGGC+TGG - chr5.1:20561584-20561603 None:intergenic 40.0%
TGTTCCCTCTTTCTACTTCT+GGG + chr5.1:20562055-20562074 MS.gene90168:intron 40.0%
TTGTGGTAAGGAAGAAGATG+TGG - chr5.1:20563819-20563838 None:intergenic 40.0%
TTGTTCCCTCTTTCTACTTC+TGG + chr5.1:20562054-20562073 MS.gene90168:intron 40.0%
TTGTTCTGCTTTGGTTATGC+AGG + chr5.1:20561420-20561439 MS.gene90168:intron 40.0%
! CGGTTGATATTGTTCTGCTT+TGG + chr5.1:20561411-20561430 MS.gene90168:intron 40.0%
!! AAACGCTGTTTTCTCTGCTA+GGG - chr5.1:20563528-20563547 None:intergenic 40.0%
!! ATCATAATTGGGCTGGTCAA+AGG - chr5.1:20561577-20561596 None:intergenic 40.0%
!! GTCTTTGTGACCGTTGATTT+AGG + chr5.1:20563668-20563687 MS.gene90168:CDS 40.0%
!! TCATAATTGGGCTGGTCAAA+GGG - chr5.1:20561576-20561595 None:intergenic 40.0%
!! TCTCAAGTAAGGGTGTTTGA+TGG - chr5.1:20563621-20563640 None:intergenic 40.0%
AACAGCGTTTGCCTTAGCAA+TGG + chr5.1:20563538-20563557 MS.gene90168:CDS 45.0%
ACACCATTTATTGGTGCACG+TGG + chr5.1:20562000-20562019 MS.gene90168:intron 45.0%
ACAGGAATATATGGCTGACG+TGG + chr5.1:20562719-20562738 MS.gene90168:intron 45.0%
CATTGGGACAATCGATCAGA+CGG + chr5.1:20561391-20561410 MS.gene90168:intron 45.0%
CATTTATTGGTGCACGTGGT+AGG + chr5.1:20562004-20562023 MS.gene90168:intron 45.0%
CTACCACGTGCACCAATAAA+TGG - chr5.1:20562006-20562025 None:intergenic 45.0%
GCAGGAGAAGATCCATTCAA+TGG + chr5.1:20561438-20561457 MS.gene90168:intron 45.0%
GGTAAGGAAGAAGATGTGGT+AGG - chr5.1:20563815-20563834 None:intergenic 45.0%
GGTGTTTGATGGAGAGATCA+TGG - chr5.1:20563610-20563629 None:intergenic 45.0%
TACGAGGATGTCTCATCGTA+CGG + chr5.1:20563719-20563738 MS.gene90168:CDS 45.0%
TACTCACATCTGTGTCTACG+AGG + chr5.1:20563703-20563722 MS.gene90168:CDS 45.0%
TACTCCTCTGTCCATTGCTA+AGG - chr5.1:20563552-20563571 None:intergenic 45.0%
TTGATGTGGCTGATTGCATG+CGG + chr5.1:20563174-20563193 MS.gene90168:intron 45.0%
TTGCTTAGTTGTCATGGCCT+TGG - chr5.1:20562105-20562124 None:intergenic 45.0%
TTTGCCTTAGCAATGGACAG+AGG + chr5.1:20563545-20563564 MS.gene90168:CDS 45.0%
! AATACCCTTTTCGTACGCCA+AGG + chr5.1:20562085-20562104 MS.gene90168:intron 45.0%
!! CAAACGCTGTTTTCTCTGCT+AGG - chr5.1:20563529-20563548 None:intergenic 45.0%
AGGAGCTGCTGAAATGTACG+AGG + chr5.1:20563640-20563659 MS.gene90168:CDS 50.0%
CAGCAGCTCCTCTCAAGTAA+GGG - chr5.1:20563631-20563650 None:intergenic 50.0%
CTTCAGCAACTGCTGTCGTT+TGG + chr5.1:20562814-20562833 MS.gene90168:CDS 50.0%
GATGCTCTTAGAGGTTGCTC+TGG + chr5.1:20561528-20561547 MS.gene90168:CDS 50.0%
GCTGCTGAAATGTACGAGGA+TGG + chr5.1:20563644-20563663 MS.gene90168:CDS 50.0%
GTGGTGTGGTTGACTGTATG+CGG + chr5.1:20563365-20563384 MS.gene90168:intron 50.0%
TCAGCAGCTCCTCTCAAGTA+AGG - chr5.1:20563632-20563651 None:intergenic 50.0%
! AGCAACTGCTGTCGTTTGGA+GGG + chr5.1:20562818-20562837 MS.gene90168:CDS 50.0%
! ATGGCCTTGGCGTACGAAAA+GGG - chr5.1:20562092-20562111 None:intergenic 50.0%
!! TAATTGGGCTGGTCAAAGGG+AGG - chr5.1:20561573-20561592 None:intergenic 50.0%
! CAGCAACTGCTGTCGTTTGG+AGG + chr5.1:20562817-20562836 MS.gene90168:CDS 55.0%
! CATGGCCTTGGCGTACGAAA+AGG - chr5.1:20562093-20562112 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 20561135 20564090 20561135 ID=MS.gene90168
chr5.1 mRNA 20561135 20564090 20561135 ID=MS.gene90168.t1;Parent=MS.gene90168
chr5.1 exon 20561135 20561264 20561135 ID=MS.gene90168.t1.exon1;Parent=MS.gene90168.t1
chr5.1 CDS 20561135 20561264 20561135 ID=cds.MS.gene90168.t1;Parent=MS.gene90168.t1
chr5.1 exon 20561442 20561596 20561442 ID=MS.gene90168.t1.exon2;Parent=MS.gene90168.t1
chr5.1 CDS 20561442 20561596 20561442 ID=cds.MS.gene90168.t1;Parent=MS.gene90168.t1
chr5.1 exon 20562723 20562914 20562723 ID=MS.gene90168.t1.exon3;Parent=MS.gene90168.t1
chr5.1 CDS 20562723 20562914 20562723 ID=cds.MS.gene90168.t1;Parent=MS.gene90168.t1
chr5.1 exon 20563503 20563840 20563503 ID=MS.gene90168.t1.exon4;Parent=MS.gene90168.t1
chr5.1 CDS 20563503 20563840 20563503 ID=cds.MS.gene90168.t1;Parent=MS.gene90168.t1
chr5.1 exon 20564009 20564090 20564009 ID=MS.gene90168.t1.exon5;Parent=MS.gene90168.t1
chr5.1 CDS 20564009 20564090 20564009 ID=cds.MS.gene90168.t1;Parent=MS.gene90168.t1
Gene Sequence

>MS.gene90168

ATGGCACCTTCTTTTGACATAACTAGTCATACTGTTTGTGTTATGGATGCTTCAGGTCAATTAGGCTTTAGCCTTGTTCAAAGACTTCTTCAAAGAGGCTATACCGTTCATGCAACCATTCAACAATATGGTACATATTATGTTATTAATAGCTTTTTATTGTTGTCAAATTCATCTTAAATGAATAAGTGGAGAATTCGAGATTTGAAACTCTGGTGACTGCATGTTTCAAATTTTTTATCAATTGAGCTAAGTTCATTGGGACAATCGATCAGACGGTTGATATTGTTCTGCTTTGGTTATGCAGGAGAAGATCCATTCAATGGGATTTCAGCTGATTCTGATAAATTAAAGATTTTTCGATCAGACCCTTTTGATTATCATAGCATAATTGATGCTCTTAGAGGTTGCTCTGGATTGTTTTACTCATTTGAACCTCCCTTTGACCAGCCCAATTATGATGTAAGCAAAAACAAAAACACCCTTTTTAACTTTTTAATCTTTTTGCTCTCTATTTCTCATATACAAATTTGAGTTAGATAAATTTATTTTATATTATATTAATATTAATCATGCATTATACATGCCCTAAGAGTCTATCTAGAATCAAACCAATGTTTATGTTACAAATTTTTATATTTACATTAAAAAAATTCATAGAAATTTGGATTTTGGAAGTACTAGTATTCATCTTCTTGGACAAGTCTTGCAATAAAAAATGATATTGATGATGTAAGAATAGTTGAAGAGTTAATGCATGAGTTTTTTTTGCTGTTGCTAGCTAGCTCTAGCTGTTGTGTGAGATGAAAAAGTAGAGTTCCTTTAAATTTGTATTAGTGCTTTATGAAAACAAGCAAGCAAGCAAACACCATTTATTGGTGCACGTGGTAGGCACCATTTACCTGAGTTTCTCACTTGCTTGTTCCCTCTTTCTACTTCTGGGATTGATCAATACCCTTTTCGTACGCCAAGGCCATGACAACTAAGCAAATTATTGTAATTTGGTTTGTACTATAGTTGATTTGATGATTTATTTGTTAAAGTTATGTCATATCATAGTCCTTGACCAAATATGTCTTAGTCATTTGAGCTGGTTAATTTTCTAATCACATGCTTATTTAATTGTATGTTATGTATATAAGTGTTTGATTATATCAACATTATCTATAAAAATATTAGCAATTATCTATTAAATTGTTAACGATCAATCATGATGGAAATTTTGATATTTGCACGCAATGTGGAACACATGCAATGATCTAAATTTTGTTACACTAACTTGATAGAATGATAGTATAAAATTAATTTTATGTCAATCATAGTATTATTGTGATTAATTTAAAGAGTTGCTACAAAAACATATGCAAATATTTTATACAGAGAATTTTTTTTTTCTTTCAAAAAAAAAATACAGAGAATTTTTTATATGTAAATTAGCATTCTCATGTCAGAATCTTTTGTAAGTTCATCCCATGTAGAAGTATTTGTTTTTACATAGTTGACAAAAGCACAACATTATTGTTTTCTAATTGATGATAACATGTCTTTTGTTTTCTAATTGATGATAACATGTCTTGATAATGAACAGGAATATATGGCTGACGTGGAAGTTAGAGCAGCACACAACGTGCTTGAAGCTTGTGCTCAAACAGAAACAATAGATAAAGTTGTGTTCACATCTTCAGCAACTGCTGTCGTTTGGAGGGAAGATCGTAAGACCATAGAACCTGATTTGGATGAAATACATTGGAGTGATGTCAATTTCTGTCGCAAATTCAAGGTATATAATAGTACTATACTTTAAACATTGTGTTTGGACTTGTCCTACTGTCCAAAACTTATAATTTCAATCATTAAATTGGTGATGCACACAATAAATAAAATAAAACTACATGACTCATGAAAAAATGATTTAGATTGTGGGTATACATCTAGATCGTTCGATCAAGATCAAATTATCGAAACCAGTCACATTTTTGTTTAAATCTAATATGTTAAAGTTTGAAATTTGGATTGTCTGATCTTTTGATCAGATGATTTTGATGTGGCTGATTGCATGCGGTCATAAAATTGTGATTTTGATTGTCCGATCAGATAAGATGATTCTGATGTGGTTGACTGCATGCAGTCATAAAATTTTGTCTTTTGATAAGATGATTCTGATGTGGCTGATTGCATGCAGTCATAAAATTGTGATTTTGATTGTCCGATCAGTTCAGATGATTCTAATGTGGTGTGGTTGACTGTATGCGGTCATAAAATTGTATGACTGCATGCAGTCATCGATTGCGACTACACAACTGACAAGCATGAAAAATAATTGATTATAGAAAAAGTTAATAATTGATTGAATTGTACAAACAAACAGTTATGGCATGGGATGTCAAAGACCCTAGCAGAGAAAACAGCGTTTGCCTTAGCAATGGACAGAGGAGTAAATATGGTATCAATCAATGCAGGACTATTGATGACCCATGATCTCTCCATCAAACACCCTTACTTGAGAGGAGCTGCTGAAATGTACGAGGATGGTGTCTTTGTGACCGTTGATTTAGGATTTTTGGTGGATACTCACATCTGTGTCTACGAGGATGTCTCATCGTACGGTCGTTATTTATGCTTTAATCACATTGTTAATACTCAAGACGACGCCGTTCAGCTTGCACATAAGTTAACACCTACCACATCTTCTTCCTTACCACAAAGGTATGACTGAATTATATGGTCAAACATTTTAACTTATAAAGGTATTCAAATAAGGTTGTTTTATGGTCAAACATTTGACTCATTCATTTAGTTTTAAGGTTGTTTTATTTGATTTTGACTATATGGACAAATAATTCAACTAAAGTTTTTTTTTTTAATGTTGTGCAGTGATGACTATGGAAAGAGTTACATTGAACAGAAAATAAGCAACAAGAAGTTGAACAAATTAATGGTGGACTTTGAGGCTTAA
Protein sequence

>MS.gene90168.t1

MAPSFDITSHTVCVMDASGQLGFSLVQRLLQRGYTVHATIQQYGEDPFNGISADSDKLKIFRSDPFDYHSIIDALRGCSGLFYSFEPPFDQPNYDEYMADVEVRAAHNVLEACAQTETIDKVVFTSSATAVVWREDRKTIEPDLDEIHWSDVNFCRKFKLWHGMSKTLAEKTAFALAMDRGVNMVSINAGLLMTHDLSIKHPYLRGAAEMYEDGVFVTVDLGFLVDTHICVYEDVSSYGRYLCFNHIVNTQDDAVQLAHKLTPTTSSSLPQSDDYGKSYIEQKISNKKLNKLMVDFEA