Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90203.t1 | XP_003608414.1 | 98.8 | 80 | 1 | 0 | 1 | 80 | 287 | 366 | 4.80E-34 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90203.t1 | Q9ZWQ9 | 48.3 | 60 | 30 | 1 | 1 | 60 | 267 | 325 | 5.6e-07 | 54.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90203.t1 | G7JV63 | 98.8 | 80 | 1 | 0 | 1 | 80 | 287 | 366 | 3.4e-34 | 153.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053562 | MS.gene90203 | 0.927569 | 9.78E-92 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90203.t1 | MTR_4g093840 | 98.750 | 80 | 1 | 0 | 1 | 80 | 287 | 366 | 2.45e-50 | 161 |
| MS.gene90203.t1 | MTR_5g065010 | 48.148 | 81 | 40 | 2 | 1 | 80 | 263 | 342 | 2.15e-18 | 77.4 |
| MS.gene90203.t1 | MTR_2g437380 | 42.500 | 80 | 45 | 1 | 1 | 80 | 184 | 262 | 6.99e-13 | 61.6 |
| MS.gene90203.t1 | MTR_2g437380 | 42.500 | 80 | 45 | 1 | 1 | 80 | 278 | 356 | 3.30e-12 | 60.1 |
| MS.gene90203.t1 | MTR_3g091360 | 37.975 | 79 | 47 | 1 | 1 | 79 | 277 | 353 | 3.69e-12 | 60.1 |
| MS.gene90203.t1 | MTR_5g032880 | 51.020 | 49 | 24 | 0 | 1 | 49 | 605 | 653 | 3.77e-12 | 60.1 |
| MS.gene90203.t1 | MTR_1g027310 | 39.506 | 81 | 47 | 2 | 1 | 80 | 237 | 316 | 1.05e-11 | 58.5 |
| MS.gene90203.t1 | MTR_3g091350 | 39.241 | 79 | 46 | 1 | 1 | 79 | 284 | 360 | 2.53e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90203.t1 | AT3G21420 | 43.750 | 80 | 45 | 0 | 1 | 80 | 283 | 362 | 2.01e-16 | 72.0 |
Find 16 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACGAACCAACCAGCTTTA+CGG | 0.305820 | 4.2:+19730194 | MS.gene90203:CDS |
| AACGAACCAACCAGCTTTAC+GGG | 0.318217 | 4.2:+19730195 | MS.gene90203:CDS |
| TACGGTTATTGCGCCATCCT+TGG | 0.472442 | 4.2:+19730079 | MS.gene90203:CDS |
| TTCATCCCAACATATGCTGC+TGG | 0.474268 | 4.2:-19730149 | None:intergenic |
| TTGTCTAGTAGTTCTGAAGC+TGG | 0.481975 | 4.2:-19730116 | None:intergenic |
| GCACAGACTTCCCGTAAAGC+TGG | 0.495291 | 4.2:-19730205 | None:intergenic |
| GCAATCCAGCAGCATATGTT+GGG | 0.503702 | 4.2:+19730144 | MS.gene90203:CDS |
| AGCAATCCAGCAGCATATGT+TGG | 0.524620 | 4.2:+19730143 | MS.gene90203:CDS |
| CATAGAGCAGCTGTGAGCAA+TGG | 0.529510 | 4.2:+19730035 | MS.gene90203:CDS |
| TTGCAATAGATTATGAGCAA+TGG | 0.541195 | 4.2:+19729993 | None:intergenic |
| TGGTTCAACCACGGTGTCCA+AGG | 0.543280 | 4.2:-19730096 | None:intergenic |
| TACAAGAATGTCATTAGCTA+CGG | 0.552606 | 4.2:+19730061 | MS.gene90203:CDS |
| TCAACCACGGTGTCCAAGGA+TGG | 0.564509 | 4.2:-19730092 | None:intergenic |
| AGACTTCCCGTAAAGCTGGT+TGG | 0.627781 | 4.2:-19730201 | None:intergenic |
| TTCTGAAGCTGGTTCAACCA+CGG | 0.645499 | 4.2:-19730105 | None:intergenic |
| TGCGCCATCCTTGGACACCG+TGG | 0.651353 | 4.2:+19730088 | MS.gene90203:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TACAAGAATGTCATTAGCTA+CGG | + | chr4.2:19730061-19730080 | MS.gene90203:CDS | 30.0% | |
| !! | TTGTCTAGTAGTTCTGAAGC+TGG | - | chr4.2:19730119-19730138 | None:intergenic | 40.0% |
| AACGAACCAACCAGCTTTAC+GGG | + | chr4.2:19730195-19730214 | MS.gene90203:CDS | 45.0% | |
| AGCAATCCAGCAGCATATGT+TGG | + | chr4.2:19730143-19730162 | MS.gene90203:CDS | 45.0% | |
| CAACGAACCAACCAGCTTTA+CGG | + | chr4.2:19730194-19730213 | MS.gene90203:CDS | 45.0% | |
| GCAATCCAGCAGCATATGTT+GGG | + | chr4.2:19730144-19730163 | MS.gene90203:CDS | 45.0% | |
| TTCATCCCAACATATGCTGC+TGG | - | chr4.2:19730152-19730171 | None:intergenic | 45.0% | |
| !! | TTCTGAAGCTGGTTCAACCA+CGG | - | chr4.2:19730108-19730127 | None:intergenic | 45.0% |
| AGACTTCCCGTAAAGCTGGT+TGG | - | chr4.2:19730204-19730223 | None:intergenic | 50.0% | |
| CATAGAGCAGCTGTGAGCAA+TGG | + | chr4.2:19730035-19730054 | MS.gene90203:CDS | 50.0% | |
| TACGGTTATTGCGCCATCCT+TGG | + | chr4.2:19730079-19730098 | MS.gene90203:CDS | 50.0% | |
| GCACAGACTTCCCGTAAAGC+TGG | - | chr4.2:19730208-19730227 | None:intergenic | 55.0% | |
| TCAACCACGGTGTCCAAGGA+TGG | - | chr4.2:19730095-19730114 | None:intergenic | 55.0% | |
| TGGTTCAACCACGGTGTCCA+AGG | - | chr4.2:19730099-19730118 | None:intergenic | 55.0% | |
| TGCGCCATCCTTGGACACCG+TGG | + | chr4.2:19730088-19730107 | MS.gene90203:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 19730005 | 19730247 | 19730005 | ID=MS.gene90203 |
| chr4.2 | mRNA | 19730005 | 19730247 | 19730005 | ID=MS.gene90203.t1;Parent=MS.gene90203 |
| chr4.2 | exon | 19730005 | 19730247 | 19730005 | ID=MS.gene90203.t1.exon1;Parent=MS.gene90203.t1 |
| chr4.2 | CDS | 19730005 | 19730247 | 19730005 | ID=cds.MS.gene90203.t1;Parent=MS.gene90203.t1 |
| Gene Sequence |
| Protein sequence |
