AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90394.t1 MTR_2g063080 97.468 553 13 1 1 553 1 552 0.0 1088
MS.gene90394.t1 MTR_2g100230 29.596 446 255 9 86 526 69 460 9.57e-50 179
MS.gene90394.t1 MTR_7g056830 30.073 409 227 7 108 515 9 359 1.32e-47 172
MS.gene90394.t1 MTR_6g082080 30.073 409 227 7 108 515 9 359 7.34e-47 170
MS.gene90394.t1 MTR_2g438110 26.039 457 270 9 87 535 10 406 2.15e-45 165
MS.gene90394.t1 MTR_6g005550 29.358 436 250 9 95 526 147 528 9.65e-45 169
MS.gene90394.t1 MTR_3g449250 29.371 429 240 11 110 536 91 458 1.95e-44 166
MS.gene90394.t1 MTR_2g043360 27.051 451 287 10 105 524 37 476 1.96e-44 166
MS.gene90394.t1 MTR_3g449250 29.371 429 240 11 110 536 91 458 2.66e-44 166
MS.gene90394.t1 MTR_7g109720 28.279 488 269 13 49 515 159 586 3.13e-44 167
MS.gene90394.t1 MTR_4g059460 26.432 454 292 10 102 524 37 479 3.77e-44 166
MS.gene90394.t1 MTR_5g090120 28.438 429 244 11 110 536 82 449 3.39e-43 162
MS.gene90394.t1 MTR_4g134170 28.837 430 255 9 104 525 282 668 4.88e-43 164
MS.gene90394.t1 MTR_4g079320 27.891 441 245 10 110 545 20 392 1.82e-42 162
MS.gene90394.t1 MTR_1g095810 28.000 425 237 11 101 514 137 503 2.89e-42 160
MS.gene90394.t1 MTR_1g026830 27.553 421 244 9 101 514 139 505 6.05e-42 159
MS.gene90394.t1 MTR_6g005320 28.605 430 254 10 104 525 244 628 5.78e-41 157
MS.gene90394.t1 MTR_2g039960 26.118 425 247 8 109 529 41 402 1.06e-40 152
MS.gene90394.t1 MTR_4g105100 27.803 446 263 10 86 526 175 566 2.87e-40 155
MS.gene90394.t1 MTR_8g018400 25.943 424 249 8 109 529 41 402 8.65e-40 150
MS.gene90394.t1 MTR_3g467550 26.712 438 246 11 103 525 111 488 1.85e-39 152
MS.gene90394.t1 MTR_4g125970 25.776 419 244 8 109 523 41 396 2.06e-39 149
MS.gene90394.t1 MTR_2g099620 25.952 420 244 9 108 523 24 380 3.14e-39 148
MS.gene90394.t1 MTR_2g099620 25.952 420 244 9 108 523 40 396 3.33e-39 148
MS.gene90394.t1 MTR_2g007000 28.571 469 245 15 97 540 486 889 3.56e-39 154
MS.gene90394.t1 MTR_4g125970 25.776 419 244 8 109 523 58 413 3.84e-39 148
MS.gene90394.t1 MTR_1g012420 26.683 416 245 7 116 529 55 412 7.67e-39 147
MS.gene90394.t1 MTR_2g032630 27.700 426 254 6 101 526 488 859 8.73e-39 152
MS.gene90394.t1 MTR_3g107420 26.118 425 255 6 107 529 45 412 1.34e-38 147
MS.gene90394.t1 MTR_2g041140 29.742 427 234 11 105 524 137 504 4.87e-38 149
MS.gene90394.t1 MTR_3g083690 28.027 446 264 9 102 535 138 538 4.93e-38 148
MS.gene90394.t1 MTR_1g054630 27.373 453 280 9 82 526 121 532 8.87e-38 147
MS.gene90394.t1 MTR_3g467550 26.777 422 234 11 119 525 96 457 1.97e-37 145
MS.gene90394.t1 MTR_6g039840 28.910 422 253 9 109 526 110 488 2.67e-37 147
MS.gene90394.t1 MTR_5g027840 29.975 407 226 12 108 509 103 455 2.69e-37 145
MS.gene90394.t1 MTR_7g057250 28.733 442 260 15 109 515 163 584 1.03e-36 145
MS.gene90394.t1 MTR_2g066050 27.108 498 279 12 67 551 5 431 1.86e-36 142
MS.gene90394.t1 MTR_3g049990 29.108 426 237 12 105 524 148 514 3.25e-36 144
MS.gene90394.t1 MTR_4g010565 26.077 418 245 8 116 529 132 489 8.13e-36 140
MS.gene90394.t1 MTR_7g076820 23.571 420 252 6 110 525 151 505 8.97e-36 140
MS.gene90394.t1 MTR_8g027775 26.526 426 258 9 110 529 103 479 1.68e-35 140
MS.gene90394.t1 MTR_4g131750 25.265 471 269 10 83 525 56 471 1.99e-35 139
MS.gene90394.t1 MTR_7g076830 23.571 420 252 7 110 525 144 498 2.55e-35 139
MS.gene90394.t1 MTR_7g076800 23.571 420 252 7 110 525 142 496 2.91e-35 139
MS.gene90394.t1 MTR_8g027770 27.635 427 252 12 110 529 202 578 5.49e-34 137
MS.gene90394.t1 MTR_4g114120 28.027 446 270 12 101 536 132 536 2.10e-33 135
MS.gene90394.t1 MTR_8g097000 27.765 425 253 14 100 517 280 657 3.40e-33 135
MS.gene90394.t1 MTR_2g055850 25.799 438 270 9 105 540 87 471 4.30e-32 130
MS.gene90394.t1 MTR_8g027780 27.189 434 262 11 102 529 98 483 8.57e-32 129
MS.gene90394.t1 MTR_7g100820 26.168 428 245 9 102 521 123 487 9.10e-32 130
MS.gene90394.t1 MTR_5g092560 29.208 404 227 11 132 531 99 447 1.82e-31 129
MS.gene90394.t1 MTR_1g102410 28.132 423 250 13 100 511 35 414 7.93e-31 127
MS.gene90394.t1 MTR_3g467550 26.196 397 218 11 103 484 111 447 2.01e-30 124
MS.gene90394.t1 MTR_4g112340 25.837 418 252 10 127 540 115 478 3.01e-29 122
MS.gene90394.t1 MTR_6g056080 29.427 384 211 14 134 511 128 457 3.88e-29 122
MS.gene90394.t1 MTR_3g071620 26.087 437 263 13 99 519 4 396 2.29e-28 119
MS.gene90394.t1 MTR_1g061970 26.813 455 241 18 102 525 72 465 5.17e-28 117
MS.gene90394.t1 MTR_1g083130 25.503 447 245 9 108 515 25 422 5.58e-28 118
MS.gene90394.t1 MTR_4g068990 28.392 398 220 11 132 522 99 438 1.59e-27 117
MS.gene90394.t1 MTR_4g105100 26.087 391 230 10 85 470 174 510 2.89e-27 115
MS.gene90394.t1 MTR_6g033070 25.522 431 246 13 98 516 131 498 1.09e-26 114
MS.gene90394.t1 MTR_2g043360 24.719 356 234 8 199 524 7 358 4.06e-25 109
MS.gene90394.t1 MTR_4g459350 29.386 228 143 7 102 322 34 250 5.76e-25 105
MS.gene90394.t1 MTR_4g049910 26.522 460 222 17 103 527 48 426 3.56e-24 105
MS.gene90394.t1 MTR_6g039840 27.679 336 203 8 195 526 13 312 7.88e-23 102
MS.gene90394.t1 MTR_8g027780 25.441 397 245 10 102 492 98 449 2.89e-22 100
MS.gene90394.t1 MTR_3g118120 24.419 430 273 10 105 521 128 518 2.58e-21 97.8
MS.gene90394.t1 MTR_3g107420 29.730 222 125 4 116 336 55 246 4.34e-21 94.7
MS.gene90394.t1 MTR_8g018370 23.196 388 214 10 138 523 2 307 5.84e-21 94.4
MS.gene90394.t1 MTR_0915s0010 22.874 341 194 7 125 461 5 280 1.85e-20 92.0
MS.gene90394.t1 MTR_4g077370 33.163 196 96 5 109 294 250 420 8.81e-20 92.4
MS.gene90394.t1 MTR_4g077370 30.303 198 84 6 109 294 2 157 4.00e-15 78.2
MS.gene90394.t1 MTR_5g077690 25.622 402 253 10 146 521 70 451 6.64e-19 90.1
MS.gene90394.t1 MTR_8g032230 36.196 163 80 4 95 257 3 141 8.33e-19 84.3
MS.gene90394.t1 MTR_1g046570 21.308 413 230 10 126 502 8 361 1.55e-17 85.1
MS.gene90394.t1 MTR_8g062720 30.052 193 117 6 109 293 91 273 4.02e-15 79.0
MS.gene90394.t1 MTR_4g112320 23.975 317 196 8 227 540 22 296 5.14e-14 74.3
MS.gene90394.t1 MTR_4g068990 26.953 256 143 6 270 522 13 227 4.97e-12 68.9
MS.gene90394.t1 MTR_6g040070 25.778 225 144 6 100 322 569 772 4.16e-11 66.2
MS.gene90394.t1 MTR_0427s0010 35.417 96 47 2 445 525 104 199 7.52e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90394.t1 AT1G12770 59.964 557 210 8 1 550 1 551 0.0 649
MS.gene90394.t1 AT1G12770 59.964 557 210 8 1 550 1 551 0.0 649
MS.gene90394.t1 AT5G60990 28.851 409 242 7 108 515 9 369 3.07e-46 169
MS.gene90394.t1 AT3G19760 25.635 433 256 9 109 535 36 408 1.03e-45 166
MS.gene90394.t1 AT1G55150 28.505 428 253 8 99 526 90 464 1.27e-45 168
MS.gene90394.t1 AT1G55150 28.505 428 253 8 99 526 115 489 1.42e-45 168
MS.gene90394.t1 AT2G47330 27.723 505 290 12 29 515 136 583 1.46e-44 169
MS.gene90394.t1 AT5G14610 28.469 418 245 6 109 526 162 525 1.19e-43 165
MS.gene90394.t1 AT5G14610 28.469 418 245 6 109 526 162 525 1.25e-43 165
MS.gene90394.t1 AT5G14610 28.469 418 245 6 109 526 170 533 1.38e-43 165
MS.gene90394.t1 AT4G09730 28.571 448 260 11 85 525 96 490 4.62e-43 163
MS.gene90394.t1 AT5G63120 29.907 428 247 10 99 526 156 530 4.87e-43 162
MS.gene90394.t1 AT1G31970 30.435 437 232 14 85 509 93 469 1.24e-42 160
MS.gene90394.t1 AT5G51280 27.924 419 245 10 101 514 139 505 3.20e-42 160
MS.gene90394.t1 AT3G01540 28.230 418 246 6 109 526 159 522 5.64e-42 160
MS.gene90394.t1 AT3G01540 28.230 418 246 6 109 526 159 522 5.64e-42 160
MS.gene90394.t1 AT3G01540 28.230 418 246 6 109 526 159 522 5.64e-42 160
MS.gene90394.t1 AT3G01540 28.230 418 246 6 109 526 159 522 6.65e-42 159
MS.gene90394.t1 AT5G26742 28.033 478 270 12 59 534 68 473 1.08e-41 160
MS.gene90394.t1 AT5G26742 28.033 478 270 12 59 534 68 473 1.09e-41 160
MS.gene90394.t1 AT5G14610 28.261 414 240 7 113 526 38 394 1.32e-41 157
MS.gene90394.t1 AT5G14610 28.261 414 240 7 113 526 38 394 1.32e-41 157
MS.gene90394.t1 AT4G33370 28.162 419 244 10 101 514 90 456 4.87e-41 156
MS.gene90394.t1 AT3G22330 27.708 480 277 13 50 525 54 467 5.90e-41 157
MS.gene90394.t1 AT3G13920 26.279 430 242 9 108 531 39 399 7.56e-41 153
MS.gene90394.t1 AT3G13920 26.279 430 242 9 108 531 43 403 8.41e-41 153
MS.gene90394.t1 AT3G06480 28.404 426 251 6 101 526 428 799 1.24e-40 158
MS.gene90394.t1 AT2G40700 25.690 471 290 11 95 524 17 468 1.57e-40 155
MS.gene90394.t1 AT5G14610 28.500 400 232 6 127 526 248 593 1.70e-40 156
MS.gene90394.t1 AT2G33730 28.477 453 255 12 77 516 292 688 1.92e-40 156
MS.gene90394.t1 AT3G13920 26.128 421 241 9 108 521 39 396 3.20e-40 151
MS.gene90394.t1 AT3G13920 26.128 421 241 9 108 521 29 386 3.53e-40 150
MS.gene90394.t1 AT1G54270 26.316 418 244 8 108 521 39 396 1.15e-39 149
MS.gene90394.t1 AT1G51380 25.971 412 238 9 108 515 22 370 1.67e-39 149
MS.gene90394.t1 AT3G13920 26.128 421 236 10 108 521 39 391 6.39e-39 147
MS.gene90394.t1 AT1G77030 27.059 425 245 9 110 529 30 394 1.61e-38 151
MS.gene90394.t1 AT2G45810 25.238 420 245 8 110 525 156 510 2.77e-38 148
MS.gene90394.t1 AT1G54270 26.316 418 239 9 108 521 39 391 3.02e-38 145
MS.gene90394.t1 AT1G72730 25.728 412 239 8 102 509 35 383 3.69e-38 145
MS.gene90394.t1 AT5G26742 28.960 404 227 10 133 534 35 380 7.17e-38 148
MS.gene90394.t1 AT1G20920 27.964 447 245 13 97 527 174 559 7.81e-38 149
MS.gene90394.t1 AT3G61240 24.048 420 250 6 110 525 126 480 8.75e-38 146
MS.gene90394.t1 AT3G61240 24.048 420 250 6 110 525 126 480 8.75e-38 146
MS.gene90394.t1 AT3G22310 26.841 421 246 10 109 525 117 479 8.80e-38 147
MS.gene90394.t1 AT3G22310 26.841 421 246 10 109 525 117 479 1.01e-37 147
MS.gene90394.t1 AT1G28180 29.880 415 213 13 137 540 2 349 1.28e-37 143
MS.gene90394.t1 AT2G42520 27.700 426 269 7 101 526 152 538 1.39e-37 147
MS.gene90394.t1 AT2G42520 27.700 426 269 7 101 526 152 538 1.39e-37 147
MS.gene90394.t1 AT2G42520 27.700 426 269 7 101 526 152 538 1.39e-37 147
MS.gene90394.t1 AT5G11170 25.962 416 248 7 116 529 55 412 2.29e-37 143
MS.gene90394.t1 AT5G11200 25.962 416 248 7 116 529 55 412 2.29e-37 143
MS.gene90394.t1 AT4G00660 23.810 420 251 7 110 525 133 487 2.74e-37 145
MS.gene90394.t1 AT4G00660 23.810 420 251 7 110 525 133 487 2.74e-37 145
MS.gene90394.t1 AT1G16280 27.400 427 242 10 106 525 56 421 3.27e-37 144
MS.gene90394.t1 AT1G20920 28.000 450 241 13 97 527 512 897 1.09e-36 146
MS.gene90394.t1 AT1G20920 28.000 450 241 13 97 527 512 897 1.09e-36 146
MS.gene90394.t1 AT1G20920 28.000 450 241 13 97 527 512 897 1.09e-36 146
MS.gene90394.t1 AT1G20920 28.000 450 241 13 97 527 512 897 1.09e-36 146
MS.gene90394.t1 AT1G20920 28.000 450 241 13 97 527 512 897 1.09e-36 146
MS.gene90394.t1 AT1G20920 28.000 450 241 13 97 527 512 897 1.09e-36 146
MS.gene90394.t1 AT3G58570 27.378 431 264 9 101 526 139 525 1.70e-36 144
MS.gene90394.t1 AT3G58510 27.465 426 266 8 102 526 145 528 2.42e-36 143
MS.gene90394.t1 AT3G58510 27.465 426 266 8 102 526 145 528 2.42e-36 143
MS.gene90394.t1 AT3G58510 27.465 426 266 8 102 526 145 528 2.42e-36 143
MS.gene90394.t1 AT5G11200 25.508 443 276 9 107 529 45 453 3.96e-36 140
MS.gene90394.t1 AT1G63250 27.314 443 273 12 86 522 304 703 3.97e-36 144
MS.gene90394.t1 AT4G16630 28.604 437 237 13 100 525 159 531 1.29e-35 142
MS.gene90394.t1 AT5G63630 27.033 455 273 11 84 530 30 433 2.53e-35 139
MS.gene90394.t1 AT5G19210 26.281 449 251 10 102 525 70 463 4.48e-35 138
MS.gene90394.t1 AT5G63630 27.033 455 273 11 84 530 224 627 9.13e-35 139
MS.gene90394.t1 AT2G07750 26.411 443 277 12 86 522 351 750 2.32e-34 139
MS.gene90394.t1 AT3G09720 27.103 428 241 10 102 521 131 495 3.00e-34 136
MS.gene90394.t1 AT3G09720 27.103 428 241 10 102 521 131 495 3.00e-34 136
MS.gene90394.t1 AT5G11200 25.727 447 269 9 116 529 55 471 2.70e-33 133
MS.gene90394.t1 AT5G14610 27.596 366 211 7 109 474 170 481 4.02e-33 132
MS.gene90394.t1 AT5G11170 25.714 385 227 6 147 529 2 329 4.51e-33 129
MS.gene90394.t1 AT5G63120 28.571 378 217 10 99 476 156 480 7.09e-32 129
MS.gene90394.t1 AT5G63120 28.571 378 217 10 99 476 156 480 7.09e-32 129
MS.gene90394.t1 AT5G63120 28.571 378 217 10 99 476 156 480 7.09e-32 129
MS.gene90394.t1 AT5G08610 27.536 414 249 10 110 517 384 752 7.93e-32 131
MS.gene90394.t1 AT3G18600 25.115 434 268 9 109 540 90 468 1.28e-31 129
MS.gene90394.t1 AT5G62190 28.704 432 240 14 96 519 88 459 4.99e-31 128
MS.gene90394.t1 AT5G08620 28.095 420 239 13 110 517 82 450 1.34e-30 126
MS.gene90394.t1 AT1G71370 28.125 416 232 12 106 509 12 372 1.49e-30 126
MS.gene90394.t1 AT5G63120 28.495 372 213 10 99 470 156 474 1.92e-30 124
MS.gene90394.t1 AT4G34910 26.207 435 231 9 106 499 44 429 4.97e-30 125
MS.gene90394.t1 AT3G06480 26.913 379 223 6 101 479 428 752 6.18e-30 125
MS.gene90394.t1 AT3G16840 26.218 431 284 10 109 515 191 611 7.52e-30 125
MS.gene90394.t1 AT3G16840 26.218 431 284 10 109 515 192 612 7.53e-30 125
MS.gene90394.t1 AT5G05450 27.546 432 248 15 102 517 8 390 1.27e-29 123
MS.gene90394.t1 AT5G65900 25.287 435 267 9 108 539 154 533 3.62e-29 122
MS.gene90394.t1 AT3G02065 25.059 427 268 8 89 514 91 466 1.61e-28 119
MS.gene90394.t1 AT3G02065 25.059 427 268 8 89 514 91 466 1.61e-28 119
MS.gene90394.t1 AT3G53110 26.180 466 256 17 102 545 84 483 2.66e-28 119
MS.gene90394.t1 AT5G54910 27.739 429 244 13 108 529 71 440 8.73e-28 118
MS.gene90394.t1 AT3G06980 24.561 456 279 13 70 502 327 740 2.97e-23 104
MS.gene90394.t1 AT3G02065 25.397 378 230 8 138 514 3 329 3.86e-22 98.6
MS.gene90394.t1 AT1G51380 25.157 318 171 9 108 421 22 276 3.20e-21 94.4
MS.gene90394.t1 AT4G15850 26.536 407 249 12 146 526 73 455 3.56e-21 97.4
MS.gene90394.t1 AT3G09620 29.078 282 152 11 97 359 380 632 2.68e-20 95.9
MS.gene90394.t1 AT3G09620 41.250 80 47 0 448 527 657 736 3.09e-11 67.0
MS.gene90394.t1 AT3G09620 29.078 282 152 11 97 359 379 631 2.75e-20 95.9
MS.gene90394.t1 AT3G09620 41.250 80 47 0 448 527 656 735 3.14e-11 67.0
MS.gene90394.t1 AT1G71280 26.963 382 187 13 100 472 10 308 2.96e-20 94.4
MS.gene90394.t1 AT1G51380 26.778 239 138 6 108 343 22 226 1.03e-19 90.5
MS.gene90394.t1 AT1G51380 26.778 239 138 6 108 343 22 226 1.14e-19 90.5
MS.gene90394.t1 AT1G71280 26.440 382 193 13 100 474 10 310 1.78e-19 90.1
MS.gene90394.t1 AT1G59990 29.278 263 153 12 50 293 14 262 2.50e-12 70.1
MS.gene90394.t1 AT1G59990 29.278 263 153 12 50 293 14 262 2.89e-12 69.7

Find 155 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTATATTGTCTGTTAT+TGG 0.115915 2.3:-31473708 MS.gene90394:CDS
AGCTCCTTTCGTTCAATTTC+CGG 0.202457 2.3:-31473362 MS.gene90394:CDS
CATGAAAACTATCACGAATT+TGG 0.207330 2.3:+31472981 None:intergenic
CACCATTCTTAAACTTCTTC+AGG 0.209602 2.3:+31472854 None:intergenic
ATCTCTTCACTTATAATCTT+TGG 0.215359 2.3:+31474029 None:intergenic
GGAAGACATGCACAGGTTAT+TGG 0.226091 2.3:-31473340 MS.gene90394:CDS
GATTATAAGTGAAGAGATTA+AGG 0.228510 2.3:-31474024 MS.gene90394:CDS
GAGAGAGTTTGAGAAGATAT+TGG 0.240727 2.3:-31473571 MS.gene90394:CDS
GAAGGGCTTTGTTGAATTCT+AGG 0.253031 2.3:-31474139 MS.gene90394:CDS
GCTGCCCTTACAACCGAAAA+AGG 0.257396 2.3:+31473231 None:intergenic
AACCGAGCGTCAGTTAATTA+TGG 0.260490 2.3:-31473268 MS.gene90394:CDS
CACATCACGGTTCTTCAAAA+TGG 0.269073 2.3:+31473776 None:intergenic
TTCCTATATTGTCTGTTATT+GGG 0.270162 2.3:-31473707 MS.gene90394:CDS
GGTGGTGATGGTGGTGAGGC+AGG 0.286614 2.3:-31473657 MS.gene90394:CDS
GAATCATACTAAGCAGTTAA+AGG 0.308011 2.3:-31472959 MS.gene90394:CDS
GCAAAGTTTCTTGTACTTGT+TGG 0.311990 2.3:-31474179 MS.gene90394:CDS
TGGTGAGGCAGGGAAGAAAT+TGG 0.315360 2.3:-31473646 MS.gene90394:CDS
ATTCAGTCGTATACGGGTTC+GGG 0.331004 2.3:-31473750 MS.gene90394:CDS
ATCTTCTTCTGAGGGTTTCA+AGG 0.339067 2.3:-31473985 MS.gene90394:CDS
TTAGCGAGAGGAAAAGTGTT+AGG 0.346027 2.3:-31473941 MS.gene90394:CDS
GCAGAGTTACATGGGGATCT+TGG 0.358850 2.3:-31472898 MS.gene90394:CDS
ATAACCTGTGCATGTCTTCC+CGG 0.363074 2.3:+31473343 None:intergenic
GCTCCTTTCGTTCAATTTCC+GGG 0.366289 2.3:-31473361 MS.gene90394:CDS
AGATATTGGGAATGGAAAAC+AGG 0.370709 2.3:-31473557 MS.gene90394:CDS
GGTGACAAATTGACCGGCTC+AGG 0.376872 2.3:+31473141 None:intergenic
AGAGAGTTTGAGAAGATATT+GGG 0.377657 2.3:-31473570 MS.gene90394:CDS
TACCCCGTCTGTGCCATCTA+AGG 0.386698 2.3:-31473106 MS.gene90394:CDS
TGTGATAATTCAGTCGTATA+CGG 0.387046 2.3:-31473757 MS.gene90394:CDS
CTTAAGGGCCTCTTCCTGCC+TGG 0.387553 2.3:+31473497 None:intergenic
CTCTCTACCACAGCCTTAGA+TGG 0.388949 2.3:+31473093 None:intergenic
AACAAACCTGCTATTGTTGT+TGG 0.392785 2.3:-31473471 MS.gene90394:CDS
TGTTGAATTCTAGGTTTGCT+TGG 0.392944 2.3:-31474130 MS.gene90394:CDS
GTGGTGATGGTGGTGAGGCA+GGG 0.404876 2.3:-31473656 MS.gene90394:CDS
AATTCAGTCGTATACGGGTT+CGG 0.405060 2.3:-31473751 MS.gene90394:CDS
TGAATTATCAGCAAGAGGTT+TGG 0.412980 2.3:-31472809 MS.gene90394:CDS
GGTGAGGCAGGGAAGAAATT+GGG 0.413210 2.3:-31473645 MS.gene90394:CDS
GGGCCTCTTCCTGCCTGGTT+CGG 0.413771 2.3:+31473502 None:intergenic
GAGATTAAGGTTAAGCCGTT+AGG 0.422743 2.3:-31474011 MS.gene90394:CDS
CAGAGTTACATGGGGATCTT+GGG 0.423541 2.3:-31472897 MS.gene90394:CDS
ATTGAGAGGTTAGGAAAAGA+GGG 0.433157 2.3:-31473840 MS.gene90394:CDS
GATTGAGAGGTTAGGAAAAG+AGG 0.433951 2.3:-31473841 MS.gene90394:CDS
TTGTAAGGGCAGCTAAAAGC+TGG 0.437170 2.3:-31473221 MS.gene90394:CDS
GCCCACCGAGCTGGTCGAAC+TGG 0.440980 2.3:-31472727 MS.gene90394:CDS
AACCTCTCAATCAACACTTC+CGG 0.442396 2.3:+31473852 None:intergenic
GAGCATGTCGGGAGGAGATC+GGG 0.449689 2.3:-31473318 MS.gene90394:CDS
AAGGTGAAAAGAAAACCCTT+TGG 0.457045 2.3:-31473966 MS.gene90394:CDS
ACCATTCTTAAACTTCTTCA+GGG 0.458193 2.3:+31472855 None:intergenic
TTTCAAAACCAACGCTTGAA+AGG 0.458359 2.3:+31474063 None:intergenic
AACCTCTGACTCTTCACATA+TGG 0.461601 2.3:+31472675 None:intergenic
AGCTAAGAAAATTGTACCGC+TGG 0.465902 2.3:-31473175 MS.gene90394:CDS
CATGGGGATCTTGGGAAGCT+TGG 0.468784 2.3:-31472889 MS.gene90394:CDS
GCTATTGTTGTTGGTACGCC+TGG 0.472411 2.3:-31473462 MS.gene90394:CDS
GGAAACTTTGTCCCCTGAGC+CGG 0.473813 2.3:-31473154 MS.gene90394:CDS
TAGCGAGAGGAAAAGTGTTA+GGG 0.474752 2.3:-31473940 MS.gene90394:CDS
ACAGGTTATTGGAGCATGTC+GGG 0.474765 2.3:-31473329 MS.gene90394:CDS
TACTCACGGCTGTCAATACT+TGG 0.475572 2.3:-31473403 MS.gene90394:CDS
GTTGCGCCTTCGAGGGAGCT+TGG 0.480698 2.3:-31473606 MS.gene90394:CDS
GAACCGAACCAGGCAGGAAG+AGG 0.481785 2.3:-31473505 MS.gene90394:CDS
GTGAAAGCTGCAGAGTTACA+TGG 0.483762 2.3:-31472907 MS.gene90394:CDS
TTCAAAACCAACGCTTGAAA+GGG 0.486260 2.3:+31474064 None:intergenic
GAAAACGACTTAGCAGCAAA+AGG 0.488039 2.3:+31473888 None:intergenic
AAAACGACTTAGCAGCAAAA+GGG 0.490677 2.3:+31473889 None:intergenic
TGGGAGGTGCGAACCGAACC+AGG 0.490783 2.3:-31473515 MS.gene90394:CDS
GTTTGAGAAGATATTGGGAA+TGG 0.495996 2.3:-31473565 MS.gene90394:CDS
CAGTGATTGTTGCGCCTTCG+AGG 0.498415 2.3:-31473614 MS.gene90394:CDS
TCTTTGACCCTTTCAAGCGT+TGG 0.501352 2.3:-31474071 MS.gene90394:CDS
CTAAGCTCGGCTATCCTACC+AGG 0.503919 2.3:+31473444 None:intergenic
GCTGGTCGAACTGGTCGGCT+TGG 0.506657 2.3:-31472718 MS.gene90394:CDS
AAAGTGGTTCAGCAGCTTGT+GGG 0.507731 2.3:-31473534 MS.gene90394:CDS
ACAAAATTACAGCACGCAAT+CGG 0.514193 2.3:+31472616 None:intergenic
GAGAATCATTTCCTGTGAGA+AGG 0.517419 2.3:-31474157 MS.gene90394:CDS
TTCCTCTCGCTAATTCCAAA+GGG 0.517871 2.3:+31473951 None:intergenic
TTCTTAGCTTGAACAAGAAG+TGG 0.518411 2.3:+31473189 None:intergenic
GGGATCTTGGGAAGCTTGGT+AGG 0.520034 2.3:-31472885 MS.gene90394:CDS
TTAGGTTTATCTTCTTCTGA+GGG 0.521787 2.3:-31473993 MS.gene90394:CDS
GGAGCATGTCGGGAGGAGAT+CGG 0.523954 2.3:-31473319 MS.gene90394:CDS
GTCTGTGCCATCTAAGGCTG+TGG 0.530947 2.3:-31473100 MS.gene90394:CDS
AAAGGATGTTGTCTTCAAGC+TGG 0.531219 2.3:-31472941 MS.gene90394:CDS
GAAAGTGGTTCAGCAGCTTG+TGG 0.532469 2.3:-31473535 MS.gene90394:CDS
GTCTTCAAGCTGGAAGCCCG+TGG 0.532671 2.3:-31472931 MS.gene90394:CDS
GTTAGGTTTATCTTCTTCTG+AGG 0.534553 2.3:-31473994 MS.gene90394:CDS
GTCGAACTGGTCGGCTTGGT+AGG 0.535009 2.3:-31472714 MS.gene90394:CDS
AGGTGCGAACCGAACCAGGC+AGG 0.536284 2.3:-31473511 MS.gene90394:CDS
TACGGGTTCGGGGAAGACAT+TGG 0.538129 2.3:-31473739 MS.gene90394:CDS
GTGATAATTCAGTCGTATAC+GGG 0.538275 2.3:-31473756 MS.gene90394:CDS
TTTCCTCTCGCTAATTCCAA+AGG 0.539879 2.3:+31473950 None:intergenic
TTCCGGAAGTGTTGATTGAG+AGG 0.541811 2.3:-31473854 MS.gene90394:CDS
CTCTGCAGCTTTCACCCCAC+GGG 0.542325 2.3:+31472915 None:intergenic
TTTCCCACTTGCACTAAGCT+CGG 0.542477 2.3:+31473431 None:intergenic
GGCCCAATAACAGACAATAT+AGG 0.542850 2.3:+31473705 None:intergenic
ATTCACTATGCCCACCGAGC+TGG 0.543015 2.3:-31472736 MS.gene90394:CDS
TAGAATCTGTCGGTAAGTCC+AGG 0.543776 2.3:+31472758 None:intergenic
CAGCCTTAGATGGCACAGAC+GGG 0.544899 2.3:+31473103 None:intergenic
AACCATAATTAACTGACGCT+CGG 0.547188 2.3:+31473266 None:intergenic
ACTGGTCGGCTTGGTAGGAA+TGG 0.547387 2.3:-31472709 MS.gene90394:CDS
CAGTAATAGTGTTTCAATGC+TGG 0.547631 2.3:+31473063 None:intergenic
AGAATCATTTCCTGTGAGAA+GGG 0.547969 2.3:-31474156 MS.gene90394:CDS
TCTTCAAGCTGGAAGCCCGT+GGG 0.549746 2.3:-31472930 MS.gene90394:CDS
GGCGTACCAACAACAATAGC+AGG 0.549925 2.3:+31473465 None:intergenic
ACTAATGAATTATCAGCAAG+AGG 0.552078 2.3:-31472814 MS.gene90394:CDS
ATTTCCGGGAAGACATGCAC+AGG 0.554633 2.3:-31473347 MS.gene90394:CDS
AGTGATTGTTGCGCCTTCGA+GGG 0.556134 2.3:-31473613 MS.gene90394:CDS
TTCAACAAAGCCCTTCTCAC+AGG 0.556564 2.3:+31474146 None:intergenic
TGTAAGGGCAGCTAAAAGCT+GGG 0.556839 2.3:-31473220 MS.gene90394:CDS
ACCAGTTCGACCAGCTCGGT+GGG 0.558237 2.3:+31472726 None:intergenic
CACAGGTTATTGGAGCATGT+CGG 0.558310 2.3:-31473330 MS.gene90394:CDS
TCTTAGCTTGAACAAGAAGT+GGG 0.560416 2.3:+31473190 None:intergenic
ACTCTGCAGCTTTCACCCCA+CGG 0.561980 2.3:+31472914 None:intergenic
TGAAAGCTGCAGAGTTACAT+GGG 0.567128 2.3:-31472906 MS.gene90394:CDS
ATGTGATCTTGTGGTAAACC+TGG 0.568642 2.3:-31472776 MS.gene90394:CDS
TTGTTGTTGGTACGCCTGGT+AGG 0.572319 2.3:-31473458 MS.gene90394:CDS
ACCCTGAAGAAGTTTAAGAA+TGG 0.575326 2.3:-31472856 MS.gene90394:CDS
GAAGTGTTGATTGAGAGGTT+AGG 0.576132 2.3:-31473849 MS.gene90394:CDS
GGGAATGGAAAACAGGAAAG+TGG 0.579120 2.3:-31473550 MS.gene90394:CDS
GAGAAAGTGTATTCATGCAC+TGG 0.579567 2.3:-31473007 MS.gene90394:CDS
TGAGAGGAGAAAATCGTGAA+GGG 0.585633 2.3:-31473680 MS.gene90394:CDS
CTTCCCGGAAATTGAACGAA+AGG 0.586913 2.3:+31473358 None:intergenic
ACAGCCTTAGATGGCACAGA+CGG 0.591630 2.3:+31473102 None:intergenic
GCCGACCAGTTCGACCAGCT+CGG 0.592616 2.3:+31472722 None:intergenic
GTGACAAATTGACCGGCTCA+GGG 0.593039 2.3:+31473142 None:intergenic
TGCATTCCAAGCTCCCTCGA+AGG 0.596605 2.3:+31473600 None:intergenic
ATAGCCGAGCTTAGTGCAAG+TGG 0.603610 2.3:-31473435 MS.gene90394:CDS
TTCAGTCGTATACGGGTTCG+GGG 0.605982 2.3:-31473749 MS.gene90394:CDS
ATCAGCAAGAGGTTTGGATG+TGG 0.606020 2.3:-31472803 MS.gene90394:CDS
GACCATATGTGAAGAGTCAG+AGG 0.611581 2.3:-31472677 MS.gene90394:CDS
TCTGTTATTGGGCCGCTGAG+AGG 0.616129 2.3:-31473696 MS.gene90394:CDS
AGTGGGAAACTTCGTACTCA+CGG 0.617308 2.3:-31473417 MS.gene90394:CDS
GTGGTTCAGCAGCTTGTGGG+AGG 0.617508 2.3:-31473531 MS.gene90394:CDS
AAACGACTTAGCAGCAAAAG+GGG 0.618984 2.3:+31473890 None:intergenic
GAAGAAGTTTAAGAATGGTG+AGG 0.624694 2.3:-31472851 MS.gene90394:CDS
CTGAGAGGAGAAAATCGTGA+AGG 0.625335 2.3:-31473681 MS.gene90394:CDS
AACCCTTTGGAATTAGCGAG+AGG 0.625999 2.3:-31473953 MS.gene90394:CDS
GACTGATGTTCAGTCTGCTG+CGG 0.626483 2.3:-31473805 MS.gene90394:CDS
GCAGCAGACTGAACATCAGT+CGG 0.632121 2.3:+31473807 None:intergenic
TAATAGTGTTTCAATGCTGG+TGG 0.632317 2.3:+31473066 None:intergenic
GACGGGGTAGAACTCGAGCT+CGG 0.635162 2.3:+31473120 None:intergenic
GAGAGGAGAAAATCGTGAAG+GGG 0.640316 2.3:-31473679 MS.gene90394:CDS
GACCAGTTCGACCAGCTCGG+TGG 0.641740 2.3:+31472725 None:intergenic
ACCGAGCTGGTCGAACTGGT+CGG 0.644486 2.3:-31472723 MS.gene90394:CDS
TCACGGTTCTTCAAAATGGT+AGG 0.651540 2.3:+31473780 None:intergenic
GCATAGTGAATAGAATCTGT+CGG 0.658249 2.3:+31472748 None:intergenic
TCAGAAGAAGATAAACCTAA+CGG 0.660328 2.3:+31473996 None:intergenic
ACGACTGAATTATCACATCA+CGG 0.662349 2.3:+31473763 None:intergenic
CTTCAAGCTGGAAGCCCGTG+GGG 0.667973 2.3:-31472929 MS.gene90394:CDS
TAGCCGAGCTTAGTGCAAGT+GGG 0.669081 2.3:-31473434 MS.gene90394:CDS
AGAGGAGAAAATCGTGAAGG+GGG 0.670062 2.3:-31473678 MS.gene90394:CDS
GAGCTCGGTGACAAATTGAC+CGG 0.674362 2.3:+31473135 None:intergenic
GTAAGGGCAGCTAAAAGCTG+GGG 0.677223 2.3:-31473219 MS.gene90394:CDS
GGTTATTGGAGCATGTCGGG+AGG 0.679100 2.3:-31473326 MS.gene90394:CDS
AGCCTTAGATGGCACAGACG+GGG 0.682132 2.3:+31473104 None:intergenic
TGTGGCAGAATGTGATCTTG+TGG 0.686568 2.3:-31472785 MS.gene90394:CDS
TGACAAATTGACCGGCTCAG+GGG 0.699585 2.3:+31473143 None:intergenic
AACACTATTACTGTGTAGTG+AGG 0.701078 2.3:-31473053 MS.gene90394:CDS
GAAAGCTGCAGAGTTACATG+GGG 0.706167 2.3:-31472905 MS.gene90394:CDS
TCAGGGGACAAAGTTTCCAG+CGG 0.716141 2.3:+31473159 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAGCATTTGAGTTA+TGG + chr2.3:31473469-31473488 None:intergenic 20.0%
! ATCTCTTCACTTATAATCTT+TGG + chr2.3:31472729-31472748 None:intergenic 25.0%
! GATTATAAGTGAAGAGATTA+AGG - chr2.3:31472731-31472750 MS.gene90394:CDS 25.0%
! TTCCTATATTGTCTGTTATT+GGG - chr2.3:31473048-31473067 MS.gene90394:CDS 25.0%
ACCATTCTTAAACTTCTTCA+GGG + chr2.3:31473903-31473922 None:intergenic 30.0%
ACTAATGAATTATCAGCAAG+AGG - chr2.3:31473941-31473960 MS.gene90394:CDS 30.0%
AGAGAGTTTGAGAAGATATT+GGG - chr2.3:31473185-31473204 MS.gene90394:CDS 30.0%
CATGAAAACTATCACGAATT+TGG + chr2.3:31473777-31473796 None:intergenic 30.0%
CTTCCTATATTGTCTGTTAT+TGG - chr2.3:31473047-31473066 MS.gene90394:CDS 30.0%
GAATCATACTAAGCAGTTAA+AGG - chr2.3:31473796-31473815 MS.gene90394:CDS 30.0%
TCAGAAGAAGATAAACCTAA+CGG + chr2.3:31472762-31472781 None:intergenic 30.0%
TGTGATAATTCAGTCGTATA+CGG - chr2.3:31472998-31473017 MS.gene90394:CDS 30.0%
TTAGGTTTATCTTCTTCTGA+GGG - chr2.3:31472762-31472781 MS.gene90394:CDS 30.0%
!! AGCAGGTTTGTTTTTCTTAA+GGG + chr2.3:31473276-31473295 None:intergenic 30.0%
!! TAGCAGGTTTGTTTTTCTTA+AGG + chr2.3:31473277-31473296 None:intergenic 30.0%
AAAACGACTTAGCAGCAAAA+GGG + chr2.3:31472869-31472888 None:intergenic 35.0%
AACAAACCTGCTATTGTTGT+TGG - chr2.3:31473284-31473303 MS.gene90394:CDS 35.0%
AACACTATTACTGTGTAGTG+AGG - chr2.3:31473702-31473721 MS.gene90394:CDS 35.0%
AACCATAATTAACTGACGCT+CGG + chr2.3:31473492-31473511 None:intergenic 35.0%
AAGGTGAAAAGAAAACCCTT+TGG - chr2.3:31472789-31472808 MS.gene90394:CDS 35.0%
ACAAAATTACAGCACGCAAT+CGG + chr2.3:31474142-31474161 None:intergenic 35.0%
ACCCTGAAGAAGTTTAAGAA+TGG - chr2.3:31473899-31473918 MS.gene90394:CDS 35.0%
ACGACTGAATTATCACATCA+CGG + chr2.3:31472995-31473014 None:intergenic 35.0%
AGAATCATTTCCTGTGAGAA+GGG - chr2.3:31472599-31472618 MS.gene90394:CDS 35.0%
AGATATTGGGAATGGAAAAC+AGG - chr2.3:31473198-31473217 MS.gene90394:CDS 35.0%
ATTGAGAGGTTAGGAAAAGA+GGG - chr2.3:31472915-31472934 MS.gene90394:CDS 35.0%
CACCATTCTTAAACTTCTTC+AGG + chr2.3:31473904-31473923 None:intergenic 35.0%
CAGTAATAGTGTTTCAATGC+TGG + chr2.3:31473695-31473714 None:intergenic 35.0%
GAAGAAGTTTAAGAATGGTG+AGG - chr2.3:31473904-31473923 MS.gene90394:CDS 35.0%
GAGAGAGTTTGAGAAGATAT+TGG - chr2.3:31473184-31473203 MS.gene90394:CDS 35.0%
GCAAAGTTTCTTGTACTTGT+TGG - chr2.3:31472576-31472595 MS.gene90394:CDS 35.0%
GCATAGTGAATAGAATCTGT+CGG + chr2.3:31474010-31474029 None:intergenic 35.0%
GTGATAATTCAGTCGTATAC+GGG - chr2.3:31472999-31473018 MS.gene90394:CDS 35.0%
GTTAGGTTTATCTTCTTCTG+AGG - chr2.3:31472761-31472780 MS.gene90394:CDS 35.0%
GTTTGAGAAGATATTGGGAA+TGG - chr2.3:31473190-31473209 MS.gene90394:CDS 35.0%
TAATAGTGTTTCAATGCTGG+TGG + chr2.3:31473692-31473711 None:intergenic 35.0%
TGAATTATCAGCAAGAGGTT+TGG - chr2.3:31473946-31473965 MS.gene90394:CDS 35.0%
TTCAAAACCAACGCTTGAAA+GGG + chr2.3:31472694-31472713 None:intergenic 35.0%
TTTCAAAACCAACGCTTGAA+AGG + chr2.3:31472695-31472714 None:intergenic 35.0%
! TCTTAGCTTGAACAAGAAGT+GGG + chr2.3:31473568-31473587 None:intergenic 35.0%
! TGTTGAATTCTAGGTTTGCT+TGG - chr2.3:31472625-31472644 MS.gene90394:CDS 35.0%
! TTCTTAGCTTGAACAAGAAG+TGG + chr2.3:31473569-31473588 None:intergenic 35.0%
!! TTTTGCTGCTAAGTCGTTTT+CGG - chr2.3:31472869-31472888 MS.gene90394:CDS 35.0%
AAACGACTTAGCAGCAAAAG+GGG + chr2.3:31472868-31472887 None:intergenic 40.0%
AAAGGATGTTGTCTTCAAGC+TGG - chr2.3:31473814-31473833 MS.gene90394:CDS 40.0%
AACCGAGCGTCAGTTAATTA+TGG - chr2.3:31473487-31473506 MS.gene90394:CDS 40.0%
AACCTCTCAATCAACACTTC+CGG + chr2.3:31472906-31472925 None:intergenic 40.0%
AACCTCTGACTCTTCACATA+TGG + chr2.3:31474083-31474102 None:intergenic 40.0%
AATTCAGTCGTATACGGGTT+CGG - chr2.3:31473004-31473023 MS.gene90394:CDS 40.0%
AGCTAAGAAAATTGTACCGC+TGG - chr2.3:31473580-31473599 MS.gene90394:CDS 40.0%
AGCTCCTTTCGTTCAATTTC+CGG - chr2.3:31473393-31473412 MS.gene90394:CDS 40.0%
ATGTGATCTTGTGGTAAACC+TGG - chr2.3:31473979-31473998 MS.gene90394:CDS 40.0%
CACATCACGGTTCTTCAAAA+TGG + chr2.3:31472982-31473001 None:intergenic 40.0%
GAAAACGACTTAGCAGCAAA+AGG + chr2.3:31472870-31472889 None:intergenic 40.0%
GAGAATCATTTCCTGTGAGA+AGG - chr2.3:31472598-31472617 MS.gene90394:CDS 40.0%
GAGATTAAGGTTAAGCCGTT+AGG - chr2.3:31472744-31472763 MS.gene90394:CDS 40.0%
GATTGAGAGGTTAGGAAAAG+AGG - chr2.3:31472914-31472933 MS.gene90394:CDS 40.0%
GGCCCAATAACAGACAATAT+AGG + chr2.3:31473053-31473072 None:intergenic 40.0%
TCACGGTTCTTCAAAATGGT+AGG + chr2.3:31472978-31472997 None:intergenic 40.0%
TGAAAGCTGCAGAGTTACAT+GGG - chr2.3:31473849-31473868 MS.gene90394:CDS 40.0%
TGAGAGGAGAAAATCGTGAA+GGG - chr2.3:31473075-31473094 MS.gene90394:CDS 40.0%
TTCCTCTCGCTAATTCCAAA+GGG + chr2.3:31472807-31472826 None:intergenic 40.0%
TTTCCTCTCGCTAATTCCAA+AGG + chr2.3:31472808-31472827 None:intergenic 40.0%
! ATCTTCTTCTGAGGGTTTCA+AGG - chr2.3:31472770-31472789 MS.gene90394:CDS 40.0%
! GAGAAAGTGTATTCATGCAC+TGG - chr2.3:31473748-31473767 MS.gene90394:CDS 40.0%
! GCGTGCTGTAATTTTGTTGA+GGG - chr2.3:31474145-31474164 MS.gene90394:CDS 40.0%
! TGCGTGCTGTAATTTTGTTG+AGG - chr2.3:31474144-31474163 MS.gene90394:CDS 40.0%
! TTCTGCAACTGTGCCTTTTT+CGG - chr2.3:31473511-31473530 MS.gene90394:CDS 40.0%
!! GAAGGGCTTTGTTGAATTCT+AGG - chr2.3:31472616-31472635 MS.gene90394:CDS 40.0%
!! GAAGTGTTGATTGAGAGGTT+AGG - chr2.3:31472906-31472925 MS.gene90394:CDS 40.0%
!! TAGCGAGAGGAAAAGTGTTA+GGG - chr2.3:31472815-31472834 MS.gene90394:CDS 40.0%
!! TTAGCGAGAGGAAAAGTGTT+AGG - chr2.3:31472814-31472833 MS.gene90394:CDS 40.0%
!!! TGTGCCTTTTTCGGTTGTAA+GGG - chr2.3:31473520-31473539 MS.gene90394:CDS 40.0%
AAAGTGGTTCAGCAGCTTGT+GGG - chr2.3:31473221-31473240 MS.gene90394:CDS 45.0%
ACAGGTTATTGGAGCATGTC+GGG - chr2.3:31473426-31473445 MS.gene90394:CDS 45.0%
AGAGGAGAAAATCGTGAAGG+GGG - chr2.3:31473077-31473096 MS.gene90394:CDS 45.0%
AGTGGGAAACTTCGTACTCA+CGG - chr2.3:31473338-31473357 MS.gene90394:CDS 45.0%
ATAACCTGTGCATGTCTTCC+CGG + chr2.3:31473415-31473434 None:intergenic 45.0%
ATCAGCAAGAGGTTTGGATG+TGG - chr2.3:31473952-31473971 MS.gene90394:CDS 45.0%
ATTCAGTCGTATACGGGTTC+GGG - chr2.3:31473005-31473024 MS.gene90394:CDS 45.0%
CACAGGTTATTGGAGCATGT+CGG - chr2.3:31473425-31473444 MS.gene90394:CDS 45.0%
CAGAGTTACATGGGGATCTT+GGG - chr2.3:31473858-31473877 MS.gene90394:CDS 45.0%
CTGAGAGGAGAAAATCGTGA+AGG - chr2.3:31473074-31473093 MS.gene90394:CDS 45.0%
CTTCCCGGAAATTGAACGAA+AGG + chr2.3:31473400-31473419 None:intergenic 45.0%
GAAAGCTGCAGAGTTACATG+GGG - chr2.3:31473850-31473869 MS.gene90394:CDS 45.0%
GACCATATGTGAAGAGTCAG+AGG - chr2.3:31474078-31474097 MS.gene90394:CDS 45.0%
GAGAGGAGAAAATCGTGAAG+GGG - chr2.3:31473076-31473095 MS.gene90394:CDS 45.0%
GCTCCTTTCGTTCAATTTCC+GGG - chr2.3:31473394-31473413 MS.gene90394:CDS 45.0%
GGAAGACATGCACAGGTTAT+TGG - chr2.3:31473415-31473434 MS.gene90394:CDS 45.0%
GGGAATGGAAAACAGGAAAG+TGG - chr2.3:31473205-31473224 MS.gene90394:CDS 45.0%
GTGAAAGCTGCAGAGTTACA+TGG - chr2.3:31473848-31473867 MS.gene90394:CDS 45.0%
TACTCACGGCTGTCAATACT+TGG - chr2.3:31473352-31473371 MS.gene90394:CDS 45.0%
TAGAATCTGTCGGTAAGTCC+AGG + chr2.3:31474000-31474019 None:intergenic 45.0%
TCTTTGACCCTTTCAAGCGT+TGG - chr2.3:31472684-31472703 MS.gene90394:CDS 45.0%
TGTAAGGGCAGCTAAAAGCT+GGG - chr2.3:31473535-31473554 MS.gene90394:CDS 45.0%
TGTGGCAGAATGTGATCTTG+TGG - chr2.3:31473970-31473989 MS.gene90394:CDS 45.0%
TTCAACAAAGCCCTTCTCAC+AGG + chr2.3:31472612-31472631 None:intergenic 45.0%
TTGTAAGGGCAGCTAAAAGC+TGG - chr2.3:31473534-31473553 MS.gene90394:CDS 45.0%
TTTCCCACTTGCACTAAGCT+CGG + chr2.3:31473327-31473346 None:intergenic 45.0%
! GTTTTCGGAACTCGGAATTC+CGG - chr2.3:31472884-31472903 MS.gene90394:CDS 45.0%
! TCACGATTTTCTCCTCTCAG+CGG + chr2.3:31473074-31473093 None:intergenic 45.0%
!! AACCCTTTGGAATTAGCGAG+AGG - chr2.3:31472802-31472821 MS.gene90394:CDS 45.0%
!! GCTAAGTCGTTTTCGGAACT+CGG - chr2.3:31472876-31472895 MS.gene90394:CDS 45.0%
!! TTCCGGAAGTGTTGATTGAG+AGG - chr2.3:31472901-31472920 MS.gene90394:CDS 45.0%
!!! CTGTGCCTTTTTCGGTTGTA+AGG - chr2.3:31473519-31473538 MS.gene90394:CDS 45.0%
ACAGCCTTAGATGGCACAGA+CGG + chr2.3:31473656-31473675 None:intergenic 50.0%
AGTGATTGTTGCGCCTTCGA+GGG - chr2.3:31473142-31473161 MS.gene90394:CDS 50.0%
ATAGCCGAGCTTAGTGCAAG+TGG - chr2.3:31473320-31473339 MS.gene90394:CDS 50.0%
ATTTCCGGGAAGACATGCAC+AGG - chr2.3:31473408-31473427 MS.gene90394:CDS 50.0%
CTCTCTACCACAGCCTTAGA+TGG + chr2.3:31473665-31473684 None:intergenic 50.0%
GAAAGTGGTTCAGCAGCTTG+TGG - chr2.3:31473220-31473239 MS.gene90394:CDS 50.0%
GACTGATGTTCAGTCTGCTG+CGG - chr2.3:31472950-31472969 MS.gene90394:CDS 50.0%
GCAGAGTTACATGGGGATCT+TGG - chr2.3:31473857-31473876 MS.gene90394:CDS 50.0%
GCAGCAGACTGAACATCAGT+CGG + chr2.3:31472951-31472970 None:intergenic 50.0%
GCTGCCCTTACAACCGAAAA+AGG + chr2.3:31473527-31473546 None:intergenic 50.0%
GGCGTACCAACAACAATAGC+AGG + chr2.3:31473293-31473312 None:intergenic 50.0%
GGTGAGGCAGGGAAGAAATT+GGG - chr2.3:31473110-31473129 MS.gene90394:CDS 50.0%
GTAAGGGCAGCTAAAAGCTG+GGG - chr2.3:31473536-31473555 MS.gene90394:CDS 50.0%
TAGCCGAGCTTAGTGCAAGT+GGG - chr2.3:31473321-31473340 MS.gene90394:CDS 50.0%
TCAGGGGACAAAGTTTCCAG+CGG + chr2.3:31473599-31473618 None:intergenic 50.0%
TGGTGAGGCAGGGAAGAAAT+TGG - chr2.3:31473109-31473128 MS.gene90394:CDS 50.0%
TTCAGTCGTATACGGGTTCG+GGG - chr2.3:31473006-31473025 MS.gene90394:CDS 50.0%
! GAGCTCGGTGACAAATTGAC+CGG + chr2.3:31473623-31473642 None:intergenic 50.0%
! GCTATTGTTGTTGGTACGCC+TGG - chr2.3:31473293-31473312 MS.gene90394:CDS 50.0%
! GTGACAAATTGACCGGCTCA+GGG + chr2.3:31473616-31473635 None:intergenic 50.0%
! TGACAAATTGACCGGCTCAG+GGG + chr2.3:31473615-31473634 None:intergenic 50.0%
! TTGTTGTTGGTACGCCTGGT+AGG - chr2.3:31473297-31473316 MS.gene90394:CDS 50.0%
AATCGTGAAGGGGGTGGTGA+TGG - chr2.3:31473086-31473105 MS.gene90394:CDS 55.0%
ACTCTGCAGCTTTCACCCCA+CGG + chr2.3:31473844-31473863 None:intergenic 55.0%
AGCCTTAGATGGCACAGACG+GGG + chr2.3:31473654-31473673 None:intergenic 55.0%
ATTCACTATGCCCACCGAGC+TGG - chr2.3:31474019-31474038 MS.gene90394:CDS 55.0%
CAGCCTTAGATGGCACAGAC+GGG + chr2.3:31473655-31473674 None:intergenic 55.0%
CAGTGATTGTTGCGCCTTCG+AGG - chr2.3:31473141-31473160 MS.gene90394:CDS 55.0%
CATGGGGATCTTGGGAAGCT+TGG - chr2.3:31473866-31473885 MS.gene90394:CDS 55.0%
CTAAGCTCGGCTATCCTACC+AGG + chr2.3:31473314-31473333 None:intergenic 55.0%
GGAAACTTTGTCCCCTGAGC+CGG - chr2.3:31473601-31473620 MS.gene90394:CDS 55.0%
GGAGAAAATCGTGAAGGGGG+TGG - chr2.3:31473080-31473099 MS.gene90394:CDS 55.0%
GGGATCTTGGGAAGCTTGGT+AGG - chr2.3:31473870-31473889 MS.gene90394:CDS 55.0%
GGTTATTGGAGCATGTCGGG+AGG - chr2.3:31473429-31473448 MS.gene90394:CDS 55.0%
TACCCCGTCTGTGCCATCTA+AGG - chr2.3:31473649-31473668 MS.gene90394:CDS 55.0%
TACGGGTTCGGGGAAGACAT+TGG - chr2.3:31473016-31473035 MS.gene90394:CDS 55.0%
TCTGTTATTGGGCCGCTGAG+AGG - chr2.3:31473059-31473078 MS.gene90394:CDS 55.0%
TCTTCAAGCTGGAAGCCCGT+GGG - chr2.3:31473825-31473844 MS.gene90394:CDS 55.0%
TGCATTCCAAGCTCCCTCGA+AGG + chr2.3:31473158-31473177 None:intergenic 55.0%
! GGTGACAAATTGACCGGCTC+AGG + chr2.3:31473617-31473636 None:intergenic 55.0%
! GTCTGTGCCATCTAAGGCTG+TGG - chr2.3:31473655-31473674 MS.gene90394:CDS 55.0%
!! ACTGGTCGGCTTGGTAGGAA+TGG - chr2.3:31474046-31474065 MS.gene90394:CDS 55.0%
ACCAGTTCGACCAGCTCGGT+GGG + chr2.3:31474032-31474051 None:intergenic 60.0%
ACCGAGCTGGTCGAACTGGT+CGG - chr2.3:31474032-31474051 MS.gene90394:CDS 60.0%
CTCTGCAGCTTTCACCCCAC+GGG + chr2.3:31473843-31473862 None:intergenic 60.0%
CTTAAGGGCCTCTTCCTGCC+TGG + chr2.3:31473261-31473280 None:intergenic 60.0%
CTTCAAGCTGGAAGCCCGTG+GGG - chr2.3:31473826-31473845 MS.gene90394:CDS 60.0%
GAACCGAACCAGGCAGGAAG+AGG - chr2.3:31473250-31473269 MS.gene90394:CDS 60.0%
GACGGGGTAGAACTCGAGCT+CGG + chr2.3:31473638-31473657 None:intergenic 60.0%
GAGCATGTCGGGAGGAGATC+GGG - chr2.3:31473437-31473456 MS.gene90394:CDS 60.0%
GGAGCATGTCGGGAGGAGAT+CGG - chr2.3:31473436-31473455 MS.gene90394:CDS 60.0%
GTCTTCAAGCTGGAAGCCCG+TGG - chr2.3:31473824-31473843 MS.gene90394:CDS 60.0%
GTGGTTCAGCAGCTTGTGGG+AGG - chr2.3:31473224-31473243 MS.gene90394:CDS 60.0%
!! GTCGAACTGGTCGGCTTGGT+AGG - chr2.3:31474041-31474060 MS.gene90394:CDS 60.0%
!! GTGGTGATGGTGGTGAGGCA+GGG - chr2.3:31473099-31473118 MS.gene90394:CDS 60.0%
AGGTGCGAACCGAACCAGGC+AGG - chr2.3:31473244-31473263 MS.gene90394:CDS 65.0%
GACCAGTTCGACCAGCTCGG+TGG + chr2.3:31474033-31474052 None:intergenic 65.0%
GCCGACCAGTTCGACCAGCT+CGG + chr2.3:31474036-31474055 None:intergenic 65.0%
GCTGGTCGAACTGGTCGGCT+TGG - chr2.3:31474037-31474056 MS.gene90394:CDS 65.0%
GGGCCTCTTCCTGCCTGGTT+CGG + chr2.3:31473256-31473275 None:intergenic 65.0%
GTTGCGCCTTCGAGGGAGCT+TGG - chr2.3:31473149-31473168 MS.gene90394:CDS 65.0%
! TGGGAGGTGCGAACCGAACC+AGG - chr2.3:31473240-31473259 MS.gene90394:CDS 65.0%
!! AGGGGGTGGTGATGGTGGTG+AGG - chr2.3:31473094-31473113 MS.gene90394:CDS 65.0%
!! CGTGAAGGGGGTGGTGATGG+TGG - chr2.3:31473089-31473108 MS.gene90394:CDS 65.0%
!! GGTGGTGATGGTGGTGAGGC+AGG - chr2.3:31473098-31473117 MS.gene90394:CDS 65.0%
GCCCACCGAGCTGGTCGAAC+TGG - chr2.3:31474028-31474047 MS.gene90394:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 31472558 31474219 31472558 ID=MS.gene90394
chr2.3 mRNA 31472558 31474219 31472558 ID=MS.gene90394.t1;Parent=MS.gene90394
chr2.3 exon 31472558 31474219 31472558 ID=MS.gene90394.t1.exon1;Parent=MS.gene90394.t1
chr2.3 CDS 31472558 31474219 31472558 ID=cds.MS.gene90394.t1;Parent=MS.gene90394.t1
Gene Sequence

>MS.gene90394

ATGCAATCTTTGGTTTCAGCAAAGTTTCTTGTACTTGTTGGAGAATCATTTCCTGTGAGAAGGGCTTTGTTGAATTCTAGGTTTGCTTGGTTTCATAGTAGTGTGAAATGTTTGAACAAACATGATTCTTTGACCCTTTCAAGCGTTGGTTTTGAAAGTGAAATTGAGCCAAAGATTATAAGTGAAGAGATTAAGGTTAAGCCGTTAGGTTTATCTTCTTCTGAGGGTTTCAAGGTGAAAAGAAAACCCTTTGGAATTAGCGAGAGGAAAAGTGTTAGGGTTAAAGAGAGAAAAGAAATAGAGAATGCCCCTTTTGCTGCTAAGTCGTTTTCGGAACTCGGAATTCCGGAAGTGTTGATTGAGAGGTTAGGAAAAGAGGGTTTTAATGTTCCGACTGATGTTCAGTCTGCTGCGGTGCCTACCATTTTGAAGAACCGTGATGTGATAATTCAGTCGTATACGGGTTCGGGGAAGACATTGGCTTATCTGCTTCCTATATTGTCTGTTATTGGGCCGCTGAGAGGAGAAAATCGTGAAGGGGGTGGTGATGGTGGTGAGGCAGGGAAGAAATTGGGAGTAGAAGCAGTGATTGTTGCGCCTTCGAGGGAGCTTGGAATGCAGATTGTGAGAGAGTTTGAGAAGATATTGGGAATGGAAAACAGGAAAGTGGTTCAGCAGCTTGTGGGAGGTGCGAACCGAACCAGGCAGGAAGAGGCCCTTAAGAAAAACAAACCTGCTATTGTTGTTGGTACGCCTGGTAGGATAGCCGAGCTTAGTGCAAGTGGGAAACTTCGTACTCACGGCTGTCAATACTTGGTATTAGATGAAGTTGACGAGCTCCTTTCGTTCAATTTCCGGGAAGACATGCACAGGTTATTGGAGCATGTCGGGAGGAGATCGGGTGCAGACCATAACTCAAATGCTAAAAAAACCGAGCGTCAGTTAATTATGGTTTCTGCAACTGTGCCTTTTTCGGTTGTAAGGGCAGCTAAAAGCTGGGGTTCTGACCCACTTCTTGTTCAAGCTAAGAAAATTGTACCGCTGGAAACTTTGTCCCCTGAGCCGGTCAATTTGTCACCGAGCTCGAGTTCTACCCCGTCTGTGCCATCTAAGGCTGTGGTAGAGAGTCTACCACCAGCATTGAAACACTATTACTGTGTAGTGAGGTTGCAGCACAAAGTTGATACATTGAGAAAGTGTATTCATGCACTGGATGCCAAATTCGTGATAGTTTTCATGAATCATACTAAGCAGTTAAAGGATGTTGTCTTCAAGCTGGAAGCCCGTGGGGTGAAAGCTGCAGAGTTACATGGGGATCTTGGGAAGCTTGGTAGGTCGACAACCCTGAAGAAGTTTAAGAATGGTGAGGTTAGAGTTCTTGTGACTAATGAATTATCAGCAAGAGGTTTGGATGTGGCAGAATGTGATCTTGTGGTAAACCTGGACTTACCGACAGATTCTATTCACTATGCCCACCGAGCTGGTCGAACTGGTCGGCTTGGTAGGAATGGTACTGTGCTGACCATATGTGAAGAGTCAGAGGTTTTTGTTGTGAGAAAATTGAAGAAGCAACTTGAAATACCGATTGCGTGCTGTAATTTTGTTGAGGGAAAACTTGCTATCACTGAAGAAGAAGAGAATGCACTGAGAAGCACGTCTTGA
Protein sequence

>MS.gene90394.t1

MQSLVSAKFLVLVGESFPVRRALLNSRFAWFHSSVKCLNKHDSLTLSSVGFESEIEPKIISEEIKVKPLGLSSSEGFKVKRKPFGISERKSVRVKERKEIENAPFAAKSFSELGIPEVLIERLGKEGFNVPTDVQSAAVPTILKNRDVIIQSYTGSGKTLAYLLPILSVIGPLRGENREGGGDGGEAGKKLGVEAVIVAPSRELGMQIVREFEKILGMENRKVVQQLVGGANRTRQEEALKKNKPAIVVGTPGRIAELSASGKLRTHGCQYLVLDEVDELLSFNFREDMHRLLEHVGRRSGADHNSNAKKTERQLIMVSATVPFSVVRAAKSWGSDPLLVQAKKIVPLETLSPEPVNLSPSSSSTPSVPSKAVVESLPPALKHYYCVVRLQHKVDTLRKCIHALDAKFVIVFMNHTKQLKDVVFKLEARGVKAAELHGDLGKLGRSTTLKKFKNGEVRVLVTNELSARGLDVAECDLVVNLDLPTDSIHYAHRAGRTGRLGRNGTVLTICEESEVFVVRKLKKQLEIPIACCNFVEGKLAITEEEENALRSTS