AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90464

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90464.t1 MTR_4g094962 74.755 408 26 4 1 333 1 406 0.0 522
MS.gene90464.t1 MTR_8g072720 97.561 82 1 1 220 301 21 101 2.39e-51 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90464.t1 AT5G67290 69.540 174 49 1 38 211 40 209 1.29e-73 233
MS.gene90464.t1 AT5G67290 72.951 122 31 1 211 330 284 405 1.66e-57 191

Find 114 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTTGTGATTGGGAAAT+TGG 0.229737 8.3:+23163776 MS.gene90464:CDS
GATGATGGCGTGCCAATTAT+AGG 0.294535 8.3:+23164313 MS.gene90464:CDS
CCAGGAATCTCTCCTATAAT+TGG 0.302323 8.3:-23164325 None:intergenic
CGTCAGCTACTTTGCCGTCT+TGG 0.313573 8.3:+23163637 MS.gene90464:CDS
GTTGCTGAGCTTGTACTTGA+TGG 0.314697 8.3:+23164421 MS.gene90464:CDS
TGTGACGGAGGCACCTTTCT+TGG 0.316760 8.3:-23163385 None:intergenic
GAGGAATCCACCTGCTTTAC+CGG 0.329508 8.3:-23163442 None:intergenic
ACTTTGCCGTCTTGGATTGA+TGG 0.340624 8.3:+23163645 MS.gene90464:CDS
TTTAGTCCTGCTAGATTTGT+TGG 0.345510 8.3:+23164472 MS.gene90464:CDS
CCGCAAACAACCACTTTCTT+CGG 0.347957 8.3:-23163324 None:intergenic
TGGATTGATGGGCCGGCCCA+TGG 0.348270 8.3:+23163657 MS.gene90464:CDS
GTGGAAATGCGTATTCTGTT+TGG 0.389369 8.3:-23163252 None:intergenic
CGCAAACAACCACTTTCTTC+GGG 0.391297 8.3:-23163323 None:intergenic
GTGGATTCCTCGCTTTAGAT+TGG 0.400406 8.3:+23163454 MS.gene90464:CDS
CACTTTCTTCGGGTGATCCA+TGG 0.400502 8.3:-23163313 None:intergenic
GCCCATGGGCCTAAAACAAT+TGG 0.415627 8.3:+23163672 MS.gene90464:CDS
TATGGTGTTGAAGTTGTGAT+TGG 0.415957 8.3:+23163768 MS.gene90464:CDS
GCCGTCTTGGATTGATGGGC+CGG 0.417639 8.3:+23163650 MS.gene90464:CDS
GGATTGATGGGCCGGCCCAT+GGG 0.421402 8.3:+23163658 MS.gene90464:CDS
TCGTTACCCCGTATCTCCTC+TGG 0.428366 8.3:-23164190 None:intergenic
CCAATTATAGGAGAGATTCC+TGG 0.432233 8.3:+23164325 MS.gene90464:CDS
CTTTGCCGTCTTGGATTGAT+GGG 0.436410 8.3:+23163646 MS.gene90464:CDS
CCACCATTGTTGATCTCAAA+CGG 0.440109 8.3:+23164449 MS.gene90464:CDS
ATTCTCAATGGTCCTGCCAC+TGG 0.441042 8.3:+23164391 MS.gene90464:CDS
AAAGTGGTTGTTTGCGGCGG+CGG 0.442611 8.3:+23163330 MS.gene90464:CDS
CTGATGATCCAGAGGAGATA+CGG 0.442639 8.3:+23164182 MS.gene90464:CDS
TTAGTCCTGCTAGATTTGTT+GGG 0.447933 8.3:+23164473 MS.gene90464:CDS
GTTGAATCGGTGGTTCTAGA+AGG 0.454207 8.3:+23163825 MS.gene90464:CDS
TTGGAGAGGTTGGTGGTGGA+TGG 0.454586 8.3:+23163795 MS.gene90464:CDS
AACATCGCTTGTGCCGCTTC+CGG 0.454824 8.3:+23163423 MS.gene90464:CDS
CGTATCTCCTCTGGATCATC+AGG 0.456119 8.3:-23164181 None:intergenic
GCAACAGCAGCTCCAGTGGC+AGG 0.457894 8.3:-23164403 None:intergenic
GTTTGCGGCGGCGGAGTCAT+CGG 0.460660 8.3:+23163339 MS.gene90464:CDS
AGATTGAACGGAATTGATAG+TGG 0.463016 8.3:-23163271 None:intergenic
CAGGGGAGGTGTATGTGTGT+GGG 0.463844 8.3:+23164137 MS.gene90464:intron
AGACCGTATCAAGCCATCTC+GGG 0.465287 8.3:+23164245 MS.gene90464:CDS
TGGGAAATTGGAGAGGTTGG+TGG 0.467331 8.3:+23163788 MS.gene90464:CDS
TAGCGACGGGACATAGTTGC+TGG 0.467406 8.3:+23164365 MS.gene90464:CDS
CACGAGCGAGATGTTCCATT+GGG 0.472582 8.3:-23163488 None:intergenic
GAAATTGGAGAGGTTGGTGG+TGG 0.474408 8.3:+23163791 MS.gene90464:CDS
CCGAAGAAAGTGGTTGTTTG+CGG 0.477983 8.3:+23163324 MS.gene90464:CDS
TCACTGTCTCAAGAGCTTAA+CGG 0.483959 8.3:+23163531 MS.gene90464:CDS
GATTGGGAAATTGGAGAGGT+TGG 0.488530 8.3:+23163785 MS.gene90464:CDS
TCCACCTGCTTTACCGGAAG+CGG 0.495731 8.3:-23163436 None:intergenic
GCTTACTTTCTCGCCAAGAA+AGG 0.496186 8.3:+23163372 MS.gene90464:CDS
CTCGCTTTAGATTGGTGTGA+CGG 0.499242 8.3:+23163462 MS.gene90464:CDS
TCAAGCCATCTCGGGGAAGA+AGG 0.503074 8.3:+23164253 MS.gene90464:CDS
TCACTCCTTCTTCCCCGAGA+TGG 0.503688 8.3:-23164258 None:intergenic
GCACGAGCGAGATGTTCCAT+TGG 0.507781 8.3:-23163489 None:intergenic
GAGAGGTTGGTGGTGGATGG+AGG 0.507834 8.3:+23163798 MS.gene90464:CDS
TCAGGGGAGGTGTATGTGTG+TGG 0.508307 8.3:+23164136 MS.gene90464:intron
TGATGATCCAGAGGAGATAC+GGG 0.515059 8.3:+23164183 MS.gene90464:CDS
GGATGGAGGAAGAGTTGAAT+CGG 0.516139 8.3:+23163812 MS.gene90464:CDS
CAATTATAGGAGAGATTCCT+GGG 0.517306 8.3:+23164326 MS.gene90464:CDS
AAGACCGTATCAAGCCATCT+CGG 0.521544 8.3:+23164244 MS.gene90464:CDS
CTCAGCAACAGCAGCTCCAG+TGG 0.525546 8.3:-23164407 None:intergenic
TGTGCCGCTTCCGGTAAAGC+AGG 0.526734 8.3:+23163432 MS.gene90464:CDS
GGAAGCGGCACAAGCGATGT+TGG 0.527319 8.3:-23163421 None:intergenic
GTGTCCGTGTAAACATTTGC+GGG 0.527352 8.3:-23163722 None:intergenic
ACTTTCTTCGGGTGATCCAT+GGG 0.531163 8.3:-23163312 None:intergenic
TTCCCCGAGATGGCTTGATA+CGG 0.532681 8.3:-23164248 None:intergenic
GGAGAGATTCCTGGGGTGAA+AGG 0.540724 8.3:+23164334 MS.gene90464:CDS
AGTTGCTGGGGTATTCTCAA+TGG 0.554810 8.3:+23164379 MS.gene90464:CDS
GATCGATAACCGTATGATTG+TGG 0.557749 8.3:-23163555 None:intergenic
AATCAATTGCGATGCTGAAG+AGG 0.563061 8.3:+23164215 MS.gene90464:CDS
AAAGGTTGTTATGTAGCGAC+GGG 0.565815 8.3:+23164352 MS.gene90464:CDS
ATGGTGTTGAAGTTGTGATT+GGG 0.571803 8.3:+23163769 MS.gene90464:CDS
GCCGCTTCCGGTAAAGCAGG+TGG 0.572469 8.3:+23163435 MS.gene90464:CDS
GTACGCCCAACAAATCTAGC+AGG 0.574585 8.3:-23164478 None:intergenic
TACCAAGAGTGACTAAGCCT+TGG 0.577916 8.3:-23164500 None:intergenic
AGTGTCCGTGTAAACATTTG+CGG 0.578489 8.3:-23163723 None:intergenic
TTGGTGTGACGGAGGCCCAA+TGG 0.578735 8.3:+23163473 MS.gene90464:CDS
CATGGATCACCCGAAGAAAG+TGG 0.588077 8.3:+23163314 MS.gene90464:CDS
GAAATGCGTATTCTGTTTGG+AGG 0.589634 8.3:-23163249 None:intergenic
AAGAAAGTGGTTGTTTGCGG+CGG 0.589660 8.3:+23163327 MS.gene90464:CDS
ATCAATTGCGATGCTGAAGA+GGG 0.593161 8.3:+23164216 MS.gene90464:CDS
GAAAGGTTGTTATGTAGCGA+CGG 0.593501 8.3:+23164351 MS.gene90464:CDS
AACACATGAAACGACGGCGC+AGG 0.595682 8.3:+23163695 MS.gene90464:CDS
AAATTCTCAATCATCGCCCA+TGG 0.597393 8.3:+23163296 MS.gene90464:CDS
GGCACGCCATCATCAGTACA+TGG 0.597713 8.3:-23164304 None:intergenic
TTGTGATTGGGAAATTGGAG+AGG 0.598568 8.3:+23163781 MS.gene90464:CDS
TAGATTTGTTGGGCGTACCA+AGG 0.600268 8.3:+23164483 MS.gene90464:CDS
AGCGACGGGACATAGTTGCT+GGG 0.602375 8.3:+23164366 MS.gene90464:CDS
ACATAACAACCTTTCACCCC+AGG 0.606403 8.3:-23164343 None:intergenic
AGAAGTACCTGATGATCCAG+AGG 0.611459 8.3:+23164174 MS.gene90464:CDS
GGATTTCTCGATGATTGTGA+CGG 0.618808 8.3:-23163400 None:intergenic
TTGATTCTTCAGTTACGGTG+AGG 0.625754 8.3:-23163593 None:intergenic
CGGGGAAGAAGGAGTGAAGG+TGG 0.626528 8.3:+23164264 MS.gene90464:CDS
TGCACCCGCAAATGTTTACA+CGG 0.626532 8.3:+23163718 MS.gene90464:CDS
GACCGTATCAAGCCATCTCG+GGG 0.629690 8.3:+23164246 MS.gene90464:CDS
CTTAACGGTCCACAATCATA+CGG 0.634820 8.3:+23163546 MS.gene90464:CDS
TTTCTCGATGATTGTGACGG+AGG 0.638358 8.3:-23163397 None:intergenic
CCGTTTGAGATCAACAATGG+TGG 0.638380 8.3:-23164449 None:intergenic
GCCGGCCCATCAATCCAAGA+CGG 0.640743 8.3:-23163651 None:intergenic
TGGAGGAAGAGTTGAATCGG+TGG 0.645010 8.3:+23163815 MS.gene90464:CDS
GCTTTAGATTGGTGTGACGG+AGG 0.647295 8.3:+23163465 MS.gene90464:CDS
TCTCGGGGAAGAAGGAGTGA+AGG 0.650707 8.3:+23164261 MS.gene90464:CDS
TTTCTTGGCGAGAAAGTAAG+CGG 0.651174 8.3:-23163370 None:intergenic
GTATGTGTGTGGGATGTCAA+AGG 0.654645 8.3:+23164147 MS.gene90464:CDS
AAACCGTTTGAGATCAACAA+TGG 0.663595 8.3:-23164452 None:intergenic
TAAGCTCTTGAGACAGTGAA+CGG 0.665750 8.3:-23163527 None:intergenic
AATTGGAACACATGAAACGA+CGG 0.667048 8.3:+23163689 MS.gene90464:CDS
GCTAATCTGTATGAATCTAG+CGG 0.674534 8.3:+23163218 MS.gene90464:CDS
CGTCACACCAATCTAAAGCG+AGG 0.676382 8.3:-23163461 None:intergenic
AGACGGCAAAGTAGCTGACG+AGG 0.677945 8.3:-23163634 None:intergenic
CGGCAAAGTAGCTGACGAGG+AGG 0.678972 8.3:-23163631 None:intergenic
TGTGTGTGGGATGTCAAAGG+AGG 0.686845 8.3:+23164150 MS.gene90464:CDS
GCGACGGGACATAGTTGCTG+GGG 0.688705 8.3:+23164367 MS.gene90464:CDS
GCTCTTGAGACAGTGAACGG+TGG 0.699538 8.3:-23163524 None:intergenic
AGTTACGGTGAGGCTGAGAG+CGG 0.699730 8.3:-23163583 None:intergenic
TGACGAGGAGGAAGAAACAG+TGG 0.711480 8.3:-23163619 None:intergenic
AATCTGTATGAATCTAGCGG+CGG 0.733075 8.3:+23163221 MS.gene90464:CDS
AATTATAGGAGAGATTCCTG+GGG 0.773408 8.3:+23164327 MS.gene90464:CDS
GATGATCCAGAGGAGATACG+GGG 0.782160 8.3:+23164184 MS.gene90464:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACATTAGTATCTATTAA+TGG - chr8.3:23164108-23164127 None:intergenic 15.0%
!!! TAATGTTTATTTTCTTTTCA+GGG + chr8.3:23164119-23164138 MS.gene90464:intron 15.0%
!! AAATGGTTTGATTCTATTTA+TGG - chr8.3:23163917-23163936 None:intergenic 20.0%
!! TTGATTGTGTATTTACTTAT+AGG + chr8.3:23163970-23163989 MS.gene90464:intron 20.0%
!!! AATGTTTATTTTCTTTTCAG+GGG + chr8.3:23164120-23164139 MS.gene90464:intron 20.0%
!!! CTAATGTTTATTTTCTTTTC+AGG + chr8.3:23164118-23164137 MS.gene90464:intron 20.0%
! AACCAAGAAATCTATGAATA+AGG - chr8.3:23163894-23163913 None:intergenic 25.0%
! ACATGGAATATGTAATTCAA+AGG - chr8.3:23164029-23164048 None:intergenic 25.0%
! ATAGAATCAAACCATTTCTT+TGG + chr8.3:23163920-23163939 MS.gene90464:intron 25.0%
! ATCCTTATTCATAGATTTCT+TGG + chr8.3:23163889-23163908 MS.gene90464:intron 25.0%
!!! TTGAGAATTTTGAGATTGAA+CGG - chr8.3:23163286-23163305 None:intergenic 25.0%
! TCATGTGTTCCAATTGTTTT+AGG - chr8.3:23163684-23163703 None:intergenic 30.0%
!! GGTTTTTGATTCTTCAGTTA+CGG - chr8.3:23163601-23163620 None:intergenic 30.0%
!! TTGAAAAGTGTCCAAAGAAA+TGG - chr8.3:23163934-23163953 None:intergenic 30.0%
AATTATAGGAGAGATTCCTG+GGG + chr8.3:23164327-23164346 MS.gene90464:CDS 35.0%
AATTGGAACACATGAAACGA+CGG + chr8.3:23163689-23163708 MS.gene90464:CDS 35.0%
AGATTGAACGGAATTGATAG+TGG - chr8.3:23163274-23163293 None:intergenic 35.0%
CAATTATAGGAGAGATTCCT+GGG + chr8.3:23164326-23164345 MS.gene90464:CDS 35.0%
GCTAATCTGTATGAATCTAG+CGG + chr8.3:23163218-23163237 MS.gene90464:CDS 35.0%
TACATATTCCATGTTCCCAT+TGG + chr8.3:23164035-23164054 MS.gene90464:intron 35.0%
TGAAGTTGTGATTGGGAAAT+TGG + chr8.3:23163776-23163795 MS.gene90464:CDS 35.0%
TTTAGTCCTGCTAGATTTGT+TGG + chr8.3:23164472-23164491 MS.gene90464:CDS 35.0%
! AAACCGTTTGAGATCAACAA+TGG - chr8.3:23164455-23164474 None:intergenic 35.0%
! ATGGTGTTGAAGTTGTGATT+GGG + chr8.3:23163769-23163788 MS.gene90464:CDS 35.0%
! TATGGTGTTGAAGTTGTGAT+TGG + chr8.3:23163768-23163787 MS.gene90464:CDS 35.0%
! TTAGTCCTGCTAGATTTGTT+GGG + chr8.3:23164473-23164492 MS.gene90464:CDS 35.0%
!! AGATTTCACTTTTGTGCCAA+TGG - chr8.3:23164054-23164073 None:intergenic 35.0%
!! GATTTCACTTTTGTGCCAAT+GGG - chr8.3:23164053-23164072 None:intergenic 35.0%
!! GTTTATTTTCTTTTCAGGGG+AGG + chr8.3:23164123-23164142 MS.gene90464:intron 35.0%
!! TTTTTGCCATGTACTGATGA+TGG + chr8.3:23164298-23164317 MS.gene90464:CDS 35.0%
AAAGGTTGTTATGTAGCGAC+GGG + chr8.3:23164352-23164371 MS.gene90464:CDS 40.0%
AAATTCTCAATCATCGCCCA+TGG + chr8.3:23163296-23163315 MS.gene90464:CDS 40.0%
AATCAATTGCGATGCTGAAG+AGG + chr8.3:23164215-23164234 MS.gene90464:CDS 40.0%
AATCTGTATGAATCTAGCGG+CGG + chr8.3:23163221-23163240 MS.gene90464:CDS 40.0%
AGTGTCCGTGTAAACATTTG+CGG - chr8.3:23163726-23163745 None:intergenic 40.0%
ATCAATTGCGATGCTGAAGA+GGG + chr8.3:23164216-23164235 MS.gene90464:CDS 40.0%
CCAATTATAGGAGAGATTCC+TGG + chr8.3:23164325-23164344 MS.gene90464:CDS 40.0%
CCACCATTGTTGATCTCAAA+CGG + chr8.3:23164449-23164468 MS.gene90464:CDS 40.0%
CCAGGAATCTCTCCTATAAT+TGG - chr8.3:23164328-23164347 None:intergenic 40.0%
CTTAACGGTCCACAATCATA+CGG + chr8.3:23163546-23163565 MS.gene90464:CDS 40.0%
GAAAGGTTGTTATGTAGCGA+CGG + chr8.3:23164351-23164370 MS.gene90464:CDS 40.0%
GAAATGCGTATTCTGTTTGG+AGG - chr8.3:23163252-23163271 None:intergenic 40.0%
GATCGATAACCGTATGATTG+TGG - chr8.3:23163558-23163577 None:intergenic 40.0%
GGATTTCTCGATGATTGTGA+CGG - chr8.3:23163403-23163422 None:intergenic 40.0%
GTGGAAATGCGTATTCTGTT+TGG - chr8.3:23163255-23163274 None:intergenic 40.0%
TAAGCTCTTGAGACAGTGAA+CGG - chr8.3:23163530-23163549 None:intergenic 40.0%
TCACTGTCTCAAGAGCTTAA+CGG + chr8.3:23163531-23163550 MS.gene90464:CDS 40.0%
TTGATTCTTCAGTTACGGTG+AGG - chr8.3:23163596-23163615 None:intergenic 40.0%
TTGTGATTGGGAAATTGGAG+AGG + chr8.3:23163781-23163800 MS.gene90464:CDS 40.0%
TTTCTTGGCGAGAAAGTAAG+CGG - chr8.3:23163373-23163392 None:intergenic 40.0%
! ACGGACACTGATTGAAAAAG+CGG + chr8.3:23163737-23163756 MS.gene90464:CDS 40.0%
!!! TCCAATTGTTTTAGGCCCAT+GGG - chr8.3:23163676-23163695 None:intergenic 40.0%
!!! TTCCAATTGTTTTAGGCCCA+TGG - chr8.3:23163677-23163696 None:intergenic 40.0%
AAGAAAGTGGTTGTTTGCGG+CGG + chr8.3:23163327-23163346 MS.gene90464:CDS 45.0%
AAGACCGTATCAAGCCATCT+CGG + chr8.3:23164244-23164263 MS.gene90464:CDS 45.0%
ACATAACAACCTTTCACCCC+AGG - chr8.3:23164346-23164365 None:intergenic 45.0%
ACTTTCTTCGGGTGATCCAT+GGG - chr8.3:23163315-23163334 None:intergenic 45.0%
ACTTTGCCGTCTTGGATTGA+TGG + chr8.3:23163645-23163664 MS.gene90464:CDS 45.0%
CCGAAGAAAGTGGTTGTTTG+CGG + chr8.3:23163324-23163343 MS.gene90464:CDS 45.0%
CCGCAAACAACCACTTTCTT+CGG - chr8.3:23163327-23163346 None:intergenic 45.0%
CGCAAACAACCACTTTCTTC+GGG - chr8.3:23163326-23163345 None:intergenic 45.0%
CTGATGATCCAGAGGAGATA+CGG + chr8.3:23164182-23164201 MS.gene90464:CDS 45.0%
CTTTGCCGTCTTGGATTGAT+GGG + chr8.3:23163646-23163665 MS.gene90464:CDS 45.0%
GAAAAAGCGGTGGAGAAGTA+TGG + chr8.3:23163750-23163769 MS.gene90464:CDS 45.0%
GATTGGGAAATTGGAGAGGT+TGG + chr8.3:23163785-23163804 MS.gene90464:CDS 45.0%
GCTTACTTTCTCGCCAAGAA+AGG + chr8.3:23163372-23163391 MS.gene90464:CDS 45.0%
GGATGGAGGAAGAGTTGAAT+CGG + chr8.3:23163812-23163831 MS.gene90464:CDS 45.0%
GTATGTGTGTGGGATGTCAA+AGG + chr8.3:23164147-23164166 MS.gene90464:CDS 45.0%
GTGTCCGTGTAAACATTTGC+GGG - chr8.3:23163725-23163744 None:intergenic 45.0%
GTTGCTGAGCTTGTACTTGA+TGG + chr8.3:23164421-23164440 MS.gene90464:CDS 45.0%
TGATGATCCAGAGGAGATAC+GGG + chr8.3:23164183-23164202 MS.gene90464:CDS 45.0%
TGCACCCGCAAATGTTTACA+CGG + chr8.3:23163718-23163737 MS.gene90464:CDS 45.0%
TTTCTCGATGATTGTGACGG+AGG - chr8.3:23163400-23163419 None:intergenic 45.0%
! AGTTGCTGGGGTATTCTCAA+TGG + chr8.3:23164379-23164398 MS.gene90464:CDS 45.0%
! CCGTTTGAGATCAACAATGG+TGG - chr8.3:23164452-23164471 None:intergenic 45.0%
! CTCGCTTTAGATTGGTGTGA+CGG + chr8.3:23163462-23163481 MS.gene90464:CDS 45.0%
! CTTTTGTGCCAATGGGAACA+TGG - chr8.3:23164046-23164065 None:intergenic 45.0%
! GACACTGATTGAAAAAGCGG+TGG + chr8.3:23163740-23163759 MS.gene90464:CDS 45.0%
! GATGATGGCGTGCCAATTAT+AGG + chr8.3:23164313-23164332 MS.gene90464:CDS 45.0%
! GTGGATTCCTCGCTTTAGAT+TGG + chr8.3:23163454-23163473 MS.gene90464:CDS 45.0%
!! AATCGGTGGTTCTAGAAGGT+GGG + chr8.3:23163829-23163848 MS.gene90464:intron 45.0%
!! AGAAGTACCTGATGATCCAG+AGG + chr8.3:23164174-23164193 MS.gene90464:CDS 45.0%
!! GTTGAATCGGTGGTTCTAGA+AGG + chr8.3:23163825-23163844 MS.gene90464:CDS 45.0%
!! TAGATTTGTTGGGCGTACCA+AGG + chr8.3:23164483-23164502 MS.gene90464:CDS 45.0%
AGACCGTATCAAGCCATCTC+GGG + chr8.3:23164245-23164264 MS.gene90464:CDS 50.0%
ATTCTCAATGGTCCTGCCAC+TGG + chr8.3:23164391-23164410 MS.gene90464:CDS 50.0%
CACGAGCGAGATGTTCCATT+GGG - chr8.3:23163491-23163510 None:intergenic 50.0%
CACTTTCTTCGGGTGATCCA+TGG - chr8.3:23163316-23163335 None:intergenic 50.0%
CATGGATCACCCGAAGAAAG+TGG + chr8.3:23163314-23163333 MS.gene90464:CDS 50.0%
CGTATCTCCTCTGGATCATC+AGG - chr8.3:23164184-23164203 None:intergenic 50.0%
GAGGAATCCACCTGCTTTAC+CGG - chr8.3:23163445-23163464 None:intergenic 50.0%
GATGATCCAGAGGAGATACG+GGG + chr8.3:23164184-23164203 MS.gene90464:CDS 50.0%
GCCCATGGGCCTAAAACAAT+TGG + chr8.3:23163672-23163691 MS.gene90464:CDS 50.0%
GTACGCCCAACAAATCTAGC+AGG - chr8.3:23164481-23164500 None:intergenic 50.0%
TGACGAGGAGGAAGAAACAG+TGG - chr8.3:23163622-23163641 None:intergenic 50.0%
TGGAGGAAGAGTTGAATCGG+TGG + chr8.3:23163815-23163834 MS.gene90464:CDS 50.0%
TGGGAAATTGGAGAGGTTGG+TGG + chr8.3:23163788-23163807 MS.gene90464:CDS 50.0%
TGTGTGTGGGATGTCAAAGG+AGG + chr8.3:23164150-23164169 MS.gene90464:CDS 50.0%
TTCCCCGAGATGGCTTGATA+CGG - chr8.3:23164251-23164270 None:intergenic 50.0%
! CGTCACACCAATCTAAAGCG+AGG - chr8.3:23163464-23163483 None:intergenic 50.0%
! GCTTTAGATTGGTGTGACGG+AGG + chr8.3:23163465-23163484 MS.gene90464:CDS 50.0%
!! ATCGGTGGTTCTAGAAGGTG+GGG + chr8.3:23163830-23163849 MS.gene90464:intron 50.0%
!! GAAATTGGAGAGGTTGGTGG+TGG + chr8.3:23163791-23163810 MS.gene90464:CDS 50.0%
!! GAATCGGTGGTTCTAGAAGG+TGG + chr8.3:23163828-23163847 MS.gene90464:intron 50.0%
!!! ATTGTTTTAGGCCCATGGGC+CGG - chr8.3:23163672-23163691 None:intergenic 50.0%
!!! GGATGCGGTGGTTTTAGCAT+TGG + chr8.3:23163866-23163885 MS.gene90464:intron 50.0%
AAAGTGGTTGTTTGCGGCGG+CGG + chr8.3:23163330-23163349 MS.gene90464:CDS 55.0%
AACACATGAAACGACGGCGC+AGG + chr8.3:23163695-23163714 MS.gene90464:CDS 55.0%
AGACGGCAAAGTAGCTGACG+AGG - chr8.3:23163637-23163656 None:intergenic 55.0%
AGCGACGGGACATAGTTGCT+GGG + chr8.3:23164366-23164385 MS.gene90464:CDS 55.0%
AGTTACGGTGAGGCTGAGAG+CGG - chr8.3:23163586-23163605 None:intergenic 55.0%
GACCGTATCAAGCCATCTCG+GGG + chr8.3:23164246-23164265 MS.gene90464:CDS 55.0%
GCACGAGCGAGATGTTCCAT+TGG - chr8.3:23163492-23163511 None:intergenic 55.0%
GCTCTTGAGACAGTGAACGG+TGG - chr8.3:23163527-23163546 None:intergenic 55.0%
GGAGAGATTCCTGGGGTGAA+AGG + chr8.3:23164334-23164353 MS.gene90464:CDS 55.0%
GGCACGCCATCATCAGTACA+TGG - chr8.3:23164307-23164326 None:intergenic 55.0%
TAGCGACGGGACATAGTTGC+TGG + chr8.3:23164365-23164384 MS.gene90464:CDS 55.0%
TCAAGCCATCTCGGGGAAGA+AGG + chr8.3:23164253-23164272 MS.gene90464:CDS 55.0%
TCACTCCTTCTTCCCCGAGA+TGG - chr8.3:23164261-23164280 None:intergenic 55.0%
TCCACCTGCTTTACCGGAAG+CGG - chr8.3:23163439-23163458 None:intergenic 55.0%
TCGTTACCCCGTATCTCCTC+TGG - chr8.3:23164193-23164212 None:intergenic 55.0%
TCTCGGGGAAGAAGGAGTGA+AGG + chr8.3:23164261-23164280 MS.gene90464:CDS 55.0%
! CAGGGGAGGTGTATGTGTGT+GGG + chr8.3:23164137-23164156 MS.gene90464:intron 55.0%
! CGTCAGCTACTTTGCCGTCT+TGG + chr8.3:23163637-23163656 MS.gene90464:CDS 55.0%
! TCAGGGGAGGTGTATGTGTG+TGG + chr8.3:23164136-23164155 MS.gene90464:intron 55.0%
!! AACATCGCTTGTGCCGCTTC+CGG + chr8.3:23163423-23163442 MS.gene90464:CDS 55.0%
!! TCGGTGGTTCTAGAAGGTGG+GGG + chr8.3:23163831-23163850 MS.gene90464:intron 55.0%
!! TCTAGAAGGTGGGGGTGTTG+TGG + chr8.3:23163839-23163858 MS.gene90464:intron 55.0%
!! TGTGACGGAGGCACCTTTCT+TGG - chr8.3:23163388-23163407 None:intergenic 55.0%
!! TTGGAGAGGTTGGTGGTGGA+TGG + chr8.3:23163795-23163814 MS.gene90464:CDS 55.0%
CGGCAAAGTAGCTGACGAGG+AGG - chr8.3:23163634-23163653 None:intergenic 60.0%
CGGGGAAGAAGGAGTGAAGG+TGG + chr8.3:23164264-23164283 MS.gene90464:CDS 60.0%
CTCAGCAACAGCAGCTCCAG+TGG - chr8.3:23164410-23164429 None:intergenic 60.0%
GCCGGCCCATCAATCCAAGA+CGG - chr8.3:23163654-23163673 None:intergenic 60.0%
GCGACGGGACATAGTTGCTG+GGG + chr8.3:23164367-23164386 MS.gene90464:CDS 60.0%
GGAAGCGGCACAAGCGATGT+TGG - chr8.3:23163424-23163443 None:intergenic 60.0%
TGTTGTGGATGCGGATGCGG+TGG + chr8.3:23163854-23163873 MS.gene90464:intron 60.0%
! GCCGTCTTGGATTGATGGGC+CGG + chr8.3:23163650-23163669 MS.gene90464:CDS 60.0%
! GGGTGTTGTGGATGCGGATG+CGG + chr8.3:23163851-23163870 MS.gene90464:intron 60.0%
! TTGGTGTGACGGAGGCCCAA+TGG + chr8.3:23163473-23163492 MS.gene90464:CDS 60.0%
!! AGGTGGGGGTGTTGTGGATG+CGG + chr8.3:23163845-23163864 MS.gene90464:intron 60.0%
!! GAGAGGTTGGTGGTGGATGG+AGG + chr8.3:23163798-23163817 MS.gene90464:CDS 60.0%
!! TGTGCCGCTTCCGGTAAAGC+AGG + chr8.3:23163432-23163451 MS.gene90464:CDS 60.0%
GCAACAGCAGCTCCAGTGGC+AGG - chr8.3:23164406-23164425 None:intergenic 65.0%
GCCGCTTCCGGTAAAGCAGG+TGG + chr8.3:23163435-23163454 MS.gene90464:CDS 65.0%
GTTTGCGGCGGCGGAGTCAT+CGG + chr8.3:23163339-23163358 MS.gene90464:CDS 65.0%
! GGATTGATGGGCCGGCCCAT+GGG + chr8.3:23163658-23163677 MS.gene90464:CDS 65.0%
! TGGATTGATGGGCCGGCCCA+TGG + chr8.3:23163657-23163676 MS.gene90464:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 23163216 23164510 23163216 ID=MS.gene90464
chr8.3 mRNA 23163216 23164510 23163216 ID=MS.gene90464.t1;Parent=MS.gene90464
chr8.3 exon 23163216 23163846 23163216 ID=MS.gene90464.t1.exon1;Parent=MS.gene90464.t1
chr8.3 CDS 23163216 23163846 23163216 ID=cds.MS.gene90464.t1;Parent=MS.gene90464.t1
chr8.3 exon 23164140 23164510 23164140 ID=MS.gene90464.t1.exon2;Parent=MS.gene90464.t1
chr8.3 CDS 23164140 23164510 23164140 ID=cds.MS.gene90464.t1;Parent=MS.gene90464.t1
Gene Sequence

>MS.gene90464

ATGCTAATCTGTATGAATCTAGCGGCGGCAATTCCTCCAAACAGAATACGCATTTCCACTATCAATTCCGTTCAATCTCAAAATTCTCAATCATCGCCCATGGATCACCCGAAGAAAGTGGTTGTTTGCGGCGGCGGAGTCATCGGCGTTTGCACCGCTTACTTTCTCGCCAAGAAAGGTGCCTCCGTCACAATCATCGAGAAATCCAACATCGCTTGTGCCGCTTCCGGTAAAGCAGGTGGATTCCTCGCTTTAGATTGGTGTGACGGAGGCCCAATGGAACATCTCGCTCGTGCAAGCTTCAATCTCCACCGTTCACTGTCTCAAGAGCTTAACGGTCCACAATCATACGGTTATCGATCACTCACCGCTCTCAGCCTCACCGTAACTGAAGAATCAAAAACCACTGTTTCTTCCTCCTCGTCAGCTACTTTGCCGTCTTGGATTGATGGGCCGGCCCATGGGCCTAAAACAATTGGAACACATGAAACGACGGCGCAGGTGCACCCGCAAATGTTTACACGGACACTGATTGAAAAAGCGGTGGAGAAGTATGGTGTTGAAGTTGTGATTGGGAAATTGGAGAGGTTGGTGGTGGATGGAGGAAGAGTTGAATCGGTGGTTCTAGAAGGTGGGGGTGTTGTGGATGCGGATGCGGTGGTTTTAGCATTGGATCCTTATTCATAGATTTCTTGGTTCCATAAATAGAATCAAACCATTTCTTTGGACACTTTTCAAATTTGAGAAATTTTAGTTGATTGTGTATTTACTTATAGGTCTATGATTCAGAATATCATTTTCTACTCGATGCCTTTGAATTACATATTCCATGTTCCCATTGGCACAAAAGTGAAATCTAAAAATAATTAAGTGTAAAGATATAACTAAGCCATTAATAGATACTAATGTTTATTTTCTTTTCAGGGGAGGTGTATGTGTGTGGGATGTCAAAGGAGGAAGAAGTACCTGATGATCCAGAGGAGATACGGGGTAACGAAGAATCAATTGCGATGCTGAAGAGGGTAGCTAAGACCGTATCAAGCCATCTCGGGGAAGAAGGAGTGAAGGTGGAACAAGCGTGCTTTTTGCCATGTACTGATGATGGCGTGCCAATTATAGGAGAGATTCCTGGGGTGAAAGGTTGTTATGTAGCGACGGGACATAGTTGCTGGGGTATTCTCAATGGTCCTGCCACTGGAGCTGCTGTTGCTGAGCTTGTACTTGATGGTCATTCCACCATTGTTGATCTCAAACGGTTTAGTCCTGCTAGATTTGTTGGGCGTACCAAGGCTTAG
Protein sequence

>MS.gene90464.t1

MLICMNLAAAIPPNRIRISTINSVQSQNSQSSPMDHPKKVVVCGGGVIGVCTAYFLAKKGASVTIIEKSNIACAASGKAGGFLALDWCDGGPMEHLARASFNLHRSLSQELNGPQSYGYRSLTALSLTVTEESKTTVSSSSSATLPSWIDGPAHGPKTIGTHETTAQVHPQMFTRTLIEKAVEKYGVEVVIGKLERLVVDGGRVESVVLEGEVYVCGMSKEEEVPDDPEEIRGNEESIAMLKRVAKTVSSHLGEEGVKVEQACFLPCTDDGVPIIGEIPGVKGCYVATGHSCWGILNGPATGAAVAELVLDGHSTIVDLKRFSPARFVGRTKA