AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90534


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90534.t1 MTR_2g081930 93.431 137 8 1 1 136 1 137 3.27e-90 271
MS.gene90534.t1 MTR_2g081960 86.131 137 17 2 1 136 1 136 4.84e-82 238
MS.gene90534.t1 MTR_4g081480 60.403 149 41 4 1 136 1 144 1.24e-60 183
MS.gene90534.t1 MTR_4g030210 35.652 115 71 2 25 136 21 135 1.08e-18 81.3
MS.gene90534.t1 MTR_4g030040 34.400 125 77 3 17 136 6 130 1.31e-16 75.5
MS.gene90534.t1 MTR_4g030040 33.333 123 79 2 17 136 7 129 1.51e-16 75.1
MS.gene90534.t1 MTR_8g039070 31.148 122 79 3 19 136 10 130 1.33e-14 69.7
MS.gene90534.t1 MTR_8g039190 30.833 120 78 3 21 136 12 130 1.42e-13 66.6
MS.gene90534.t1 MTR_8g447340 29.851 134 85 3 12 136 8 141 1.42e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90534.t1 AT3G20660 66.154 130 40 3 10 136 10 138 9.38e-58 187
MS.gene90534.t1 AT1G73220 36.283 113 71 1 25 136 38 150 3.68e-20 85.5
MS.gene90534.t1 AT1G79360 30.579 121 81 2 19 136 7 127 5.88e-15 70.5
MS.gene90534.t1 AT1G16390 32.353 136 79 3 11 136 3 135 1.73e-14 69.3
MS.gene90534.t1 AT1G79410 29.323 133 89 5 8 136 1 132 8.30e-13 64.3
MS.gene90534.t1 AT1G16370 25.547 137 94 4 8 136 1 137 4.30e-12 62.4

Find 0 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GATTTGTGGTGATAAGTTTA+AGG + 14456:16447-16466 MS.gene90534:CDS 30.0%
! GGTTATGATTTTTGCTGATA+GGG + 14456:16303-16322 MS.gene90534:CDS 30.0%
! TGGTTATGATTTTTGCTGAT+AGG + 14456:16302-16321 MS.gene90534:CDS 30.0%
! TTTGGAAGCTTTTCATACTA+TGG + 14456:16282-16301 MS.gene90534:CDS 30.0%
!!! GATTTTTTCAGTTTCATTGG+TGG - 14456:16176-16195 MS.gene90534:intergenic 30.0%
!!! GCAGATTTTTTCAGTTTCAT+TGG - 14456:16179-16198 MS.gene90534:intergenic 30.0%
CATTCTAACAAGTCTTGCTT+GGG + 14456:16258-16277 MS.gene90534:CDS 35.0%
TCATTCTAACAAGTCTTGCT+TGG + 14456:16257-16276 MS.gene90534:CDS 35.0%
! TGTGGTGATAAGTTTAAGGT+TGG + 14456:16451-16470 MS.gene90534:CDS 35.0%
! TTCAAGCACTTTTCTTTGCT+GGG + 14456:16479-16498 MS.gene90534:CDS 35.0%
!! TCATTGGTGGTTTTTGATGA+TGG - 14456:16163-16182 MS.gene90534:intergenic 35.0%
!! TCTGAATGGAGTTTGATTTG+TGG + 14456:16433-16452 MS.gene90534:CDS 35.0%
!! TTTGATGATGGTGGTAAGAT+CGG - 14456:16151-16170 MS.gene90534:intergenic 35.0%
ACTGTGGTGAGTTTGGAAAA+TGG + 14456:16221-16240 MS.gene90534:CDS 40.0%
GAGAAATACTGTGGTGAGTT+TGG + 14456:16214-16233 MS.gene90534:CDS 40.0%
GAGATGCTTGAGAAATACTG+TGG + 14456:16205-16224 MS.gene90534:CDS 40.0%
TTCTTTGCTGGGTGTATGAT+TGG + 14456:16490-16509 MS.gene90534:CDS 40.0%
! GTTCAAGCACTTTTCTTTGC+TGG + 14456:16478-16497 MS.gene90534:CDS 40.0%
! TGAGTTGAGATCATCGGAAA+GGG - 14456:16128-16147 MS.gene90534:intergenic 40.0%
! TTGGAAGTGTGTTGAAGGTA+TGG + 14456:16333-16352 MS.gene90534:CDS 40.0%
!!! TTGGTGGTTTTTGATGATGG+TGG - 14456:16160-16179 MS.gene90534:intergenic 40.0%
AACAAGTCTTGCTTGGGCTT+TGG + 14456:16264-16283 MS.gene90534:CDS 45.0%
ATGCTTCTACGGTGTCTGAA+TGG + 14456:16419-16438 MS.gene90534:CDS 45.0%
CCTTCAACACACTTCCAATC+AGG - 14456:16331-16350 MS.gene90534:intergenic 45.0%
TTGCTGATAGGGAACCTGAT+TGG + 14456:16314-16333 MS.gene90534:CDS 45.0%
! CCTGATTGGAAGTGTGTTGA+AGG + 14456:16328-16347 MS.gene90534:CDS 45.0%
! GATCGGTGAGTTGAGATCAT+CGG - 14456:16134-16153 MS.gene90534:intergenic 45.0%
! GTGAGTTGAGATCATCGGAA+AGG - 14456:16129-16148 MS.gene90534:intergenic 45.0%
!!! CACCGTAGAAGCATCTTTTC+CGG - 14456:16413-16432 MS.gene90534:intergenic 45.0%
CATATCGCAGACACTTCCAC+CGG - 14456:16368-16387 MS.gene90534:intergenic 50.0%
CGCCGGAAAAGATGCTTCTA+CGG + 14456:16408-16427 MS.gene90534:CDS 50.0%
ATATGTCGTCGTCGTGGCAG+TGG + 14456:16383-16402 MS.gene90534:CDS 55.0%
TCTGCGATATGTCGTCGTCG+TGG + 14456:16377-16396 MS.gene90534:CDS 55.0%
! GAAGGTATGGAGTGTTCCGC+CGG + 14456:16346-16365 MS.gene90534:CDS 55.0%
ATCGCAGACACTTCCACCGG+CGG - 14456:16365-16384 MS.gene90534:intergenic 60.0%
TCGTCGTGGCAGTGGATCGC+CGG + 14456:16391-16410 MS.gene90534:CDS 65.0%
! GGTATGGAGTGTTCCGCCGG+TGG + 14456:16349-16368 MS.gene90534:CDS 65.0%


Chromosome Type Strat End Strand Name
14456 gene 16106 16516 16106 ID=MS.gene90534
14456 mRNA 16106 16516 16106 ID=MS.gene90534.t1;Parent=MS.gene90534
14456 exon 16106 16516 16106 ID=MS.gene90534.t1.exon1;Parent=MS.gene90534.t1
14456 CDS 16106 16516 16106 ID=cds.MS.gene90534.t1;Parent=MS.gene90534.t1
Gene Sequence

>MS.gene90534

ATGTCGGCTACATTCTCATCCCTTTCCGATGATCTCAACTCACCGATCTTACCACCATCATCAAAAACCACCAATGAAACTGAAAAAATCTGCATCGATGAGATGCTTGAGAAATACTGTGGTGAGTTTGGAAAATGGCAGCTGAAACACTTCATTCTAACAAGTCTTGCTTGGGCTTTGGAAGCTTTTCATACTATGGTTATGATTTTTGCTGATAGGGAACCTGATTGGAAGTGTGTTGAAGGTATGGAGTGTTCCGCCGGTGGAAGTGTCTGCGATATGTCGTCGTCGTGGCAGTGGATCGCCGGAAAAGATGCTTCTACGGTGTCTGAATGGAGTTTGATTTGTGGTGATAAGTTTAAGGTTGGACTTGTTCAAGCACTTTTCTTTGCTGGGTGTATGATTGGTTAG

Protein sequence

>MS.gene90534.t1

MSATFSSLSDDLNSPILPPSSKTTNETEKICIDEMLEKYCGEFGKWQLKHFILTSLAWALEAFHTMVMIFADREPDWKCVEGMECSAGGSVCDMSSSWQWIAGKDASTVSEWSLICGDKFKVGLVQALFFAGCMIG