Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90607.t1 | XP_003616815.1 | 89.9 | 179 | 5 | 3 | 1 | 169 | 1 | 176 | 5.20E-38 | 167.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90607.t1 | Q07202 | 61.3 | 155 | 22 | 2 | 1 | 117 | 1 | 155 | 3.4e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90607.t1 | G7K7E3 | 89.9 | 179 | 5 | 3 | 1 | 169 | 1 | 176 | 3.7e-38 | 167.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059894 | MS.gene90607 | 0.809211 | 2.12E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90607.t1 | MTR_5g084570 | 69.274 | 179 | 42 | 3 | 1 | 169 | 1 | 176 | 2.07e-43 | 142 |
| MS.gene90607.t1 | MTR_5g084530 | 56.944 | 144 | 45 | 4 | 1 | 140 | 1 | 131 | 1.86e-17 | 74.3 |
| MS.gene90607.t1 | MTR_5g084530 | 80.000 | 50 | 7 | 1 | 1 | 47 | 1 | 50 | 1.96e-13 | 64.7 |
| MS.gene90607.t1 | MTR_5g084470 | 77.551 | 49 | 8 | 1 | 1 | 46 | 1 | 49 | 1.01e-11 | 60.8 |
| MS.gene90607.t1 | MTR_5g084460 | 81.633 | 49 | 6 | 1 | 1 | 46 | 1 | 49 | 1.09e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 65 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATTACCACCACCATATT+TGG | 0.192353 | 5.4:+66893624 | None:intergenic |
| AACTTCCTCTGATGCCAAAA+AGG | 0.230354 | 5.4:-66894251 | MS.gene90607:CDS |
| ATTGTGGTAACCGCCATATT+TGG | 0.317393 | 5.4:+66893822 | None:intergenic |
| GAAGTAAACGATGCCAAATA+TGG | 0.333960 | 5.4:-66893835 | MS.gene90607:CDS |
| GAAGTAAACGATGCCAAATA+TGG | 0.342429 | 5.4:-66893637 | MS.gene90607:CDS |
| AATGGTGGTGGACATTACTA+TGG | 0.429163 | 5.4:-66893598 | MS.gene90607:CDS |
| AATGGTGGTGGACATTACTA+TGG | 0.429163 | 5.4:-66893781 | MS.gene90607:CDS |
| TGGTTCTTCTTGTGTCAGCT+AGG | 0.433566 | 5.4:-66894285 | MS.gene90607:CDS |
| AATCCTCATCCTAGGCCTCT+TGG | 0.458683 | 5.4:-66894311 | MS.gene90607:CDS |
| GAACCATTGCCAAGAGGCCT+AGG | 0.460802 | 5.4:+66894302 | None:intergenic |
| GGTGGTTCCCACCACGGTGG+AGG | 0.469523 | 5.4:-66893739 | MS.gene90607:CDS |
| GGTGGTTCTCACCATGGTGG+AGG | 0.471933 | 5.4:-66893553 | MS.gene90607:CDS |
| GGTGGTTCTCACGGTGGAGG+TGG | 0.475576 | 5.4:-66893757 | MS.gene90607:CDS |
| GGTTCTCACCATGGTGGAGG+TGG | 0.480643 | 5.4:-66893550 | MS.gene90607:CDS |
| GGTTCCCACCACGGTGGAGG+TGG | 0.484027 | 5.4:-66893736 | MS.gene90607:CDS |
| GGTGGTTCTCACCACGGTGG+AGG | 0.484853 | 5.4:-66893574 | MS.gene90607:CDS |
| CATCCTAGGCCTCTTGGCAA+TGG | 0.496291 | 5.4:-66894305 | MS.gene90607:CDS |
| GGAGGTGGTTCTCACGGTGG+AGG | 0.512830 | 5.4:-66893760 | MS.gene90607:CDS |
| GGTTCTTCTTGTGTCAGCTA+GGG | 0.514897 | 5.4:-66894284 | MS.gene90607:CDS |
| GGTTCTCACCACGGTGGAGG+TGG | 0.520241 | 5.4:-66893571 | MS.gene90607:CDS |
| GCAACCACCTCCACCGTGGT+GGG | 0.523248 | 5.4:+66893732 | None:intergenic |
| TACTATGGAGGTGGTTCTCA+CGG | 0.525230 | 5.4:-66893766 | MS.gene90607:CDS |
| ATTCAGCATGTGAGCAGCAA+TGG | 0.536032 | 5.4:+66893506 | None:intergenic |
| TTGCCAAGAGGCCTAGGATG+AGG | 0.538496 | 5.4:+66894308 | None:intergenic |
| GGTAATTACCACAATGGTGG+TGG | 0.547595 | 5.4:-66893610 | MS.gene90607:CDS |
| GGTAATTACCACAATGGTGG+TGG | 0.547595 | 5.4:-66893793 | MS.gene90607:CDS |
| CATGTGAGCAGCAATGGCCG+TGG | 0.551382 | 5.4:+66893512 | None:intergenic |
| GGCGGTTACCACAATGGTGG+TGG | 0.554658 | 5.4:-66893814 | MS.gene90607:CDS |
| GGTGGACATTACTATGGAGG+TGG | 0.556814 | 5.4:-66893592 | MS.gene90607:CDS |
| GGTGGACATTACTATGGAGG+TGG | 0.556814 | 5.4:-66893775 | MS.gene90607:CDS |
| CATATGAGCAGCAATGGCCG+TGG | 0.561023 | 5.4:+66893698 | None:intergenic |
| AACGATGCCAAATATGGTGG+TGG | 0.579616 | 5.4:-66893631 | MS.gene90607:CDS |
| GGCAACCACCTCCACCGTGG+TGG | 0.590734 | 5.4:+66893731 | None:intergenic |
| GTTTGTTGCCGTGCAAGCTG+AGG | 0.597485 | 5.4:-66893671 | MS.gene90607:CDS |
| GGTTGCCATTACTATTGCCA+CGG | 0.599690 | 5.4:-66893529 | MS.gene90607:CDS |
| AATGGCCGTGGCAATAGTAA+TGG | 0.601894 | 5.4:+66893524 | None:intergenic |
| AAATATGGCGGTTACCACAA+TGG | 0.601953 | 5.4:-66893820 | MS.gene90607:CDS |
| ACTCGGCATATGAGCAGCAA+TGG | 0.602148 | 5.4:+66893692 | None:intergenic |
| AGCTTGCACGGCAACAAACT+CGG | 0.602417 | 5.4:+66893675 | None:intergenic |
| GGTAATTACCACCACCATTG+TGG | 0.604720 | 5.4:+66893806 | None:intergenic |
| GGTGGAGGTGGTTCCCACCA+CGG | 0.608637 | 5.4:-66893745 | MS.gene90607:CDS |
| GGTGGAGGTGGTTCTCACCA+TGG | 0.610718 | 5.4:-66893559 | MS.gene90607:CDS |
| GGTTGCCATCACTATTGCCA+CGG | 0.613013 | 5.4:-66893715 | MS.gene90607:CDS |
| AATGGCCGTGGCAATAGTGA+TGG | 0.615442 | 5.4:+66893710 | None:intergenic |
| AGTAATGTCCACCACCATTG+TGG | 0.641701 | 5.4:+66893602 | None:intergenic |
| AGTAATGTCCACCACCATTG+TGG | 0.641701 | 5.4:+66893785 | None:intergenic |
| TATGGAGGTGGTTCTCACCA+CGG | 0.648357 | 5.4:-66893580 | MS.gene90607:CDS |
| GGAGGTGGTTCTCACCATGG+TGG | 0.655820 | 5.4:-66893556 | MS.gene90607:CDS |
| GGAGGTGGTTCCCACCACGG+TGG | 0.673714 | 5.4:-66893742 | MS.gene90607:CDS |
| ATTTGTTGCCGTGCAAGCTG+AGG | 0.673816 | 5.4:-66893485 | MS.gene90607:CDS |
| CAAGAAGAACCATTGCCAAG+AGG | 0.674082 | 5.4:+66894296 | None:intergenic |
| AATGGCAACCACCTCCACCA+TGG | 0.681347 | 5.4:+66893542 | None:intergenic |
| GGTGGTAATTACCACAATGG+TGG | 0.685453 | 5.4:-66893613 | MS.gene90607:CDS |
| GGTGGTAATTACCACAATGG+TGG | 0.685453 | 5.4:-66893796 | MS.gene90607:CDS |
| GGTGGTGGACATTACTATGG+AGG | 0.685934 | 5.4:-66893595 | MS.gene90607:CDS |
| GGTGGTGGACATTACTATGG+AGG | 0.685934 | 5.4:-66893778 | MS.gene90607:CDS |
| GTAAACGATGCCAAATATGG+CGG | 0.688367 | 5.4:-66893832 | MS.gene90607:CDS |
| TATGGAGGTGGTTCTCACGG+TGG | 0.695015 | 5.4:-66893763 | MS.gene90607:CDS |
| TATGGCGGTTACCACAATGG+TGG | 0.720585 | 5.4:-66893817 | MS.gene90607:CDS |
| GGTGGTGGTAATTACCACAA+TGG | 0.736293 | 5.4:-66893616 | MS.gene90607:CDS |
| GGTGGTGGTAATTACCACAA+TGG | 0.736293 | 5.4:-66893799 | MS.gene90607:CDS |
| GTAAACGATGCCAAATATGG+TGG | 0.739461 | 5.4:-66893634 | MS.gene90607:CDS |
| GGAGGTGGTTCTCACCACGG+TGG | 0.747901 | 5.4:-66893577 | MS.gene90607:CDS |
| GATGGCAACCACCTCCACCG+TGG | 0.754549 | 5.4:+66893728 | None:intergenic |
| GGTGAGAACCACCTCCACCG+TGG | 0.755651 | 5.4:+66893563 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTATATATATAAAATGTAA+TGG | - | chr5.4:66893896-66893915 | MS.gene90607:intron | 10.0% |
| ! | CATATATATTATGTTTGCAG+AGG | - | chr5.4:66893921-66893940 | MS.gene90607:intron | 25.0% |
| !!! | ACTGCTAGCTTTATAAAATA+AGG | - | chr5.4:66893798-66893817 | MS.gene90607:CDS | 25.0% |
| GAAGTAAACGATGCCAAATA+TGG | - | chr5.4:66893961-66893980 | MS.gene90607:intron | 35.0% | |
| GTAATTACCACCACCATATT+TGG | + | chr5.4:66894175-66894194 | None:intergenic | 35.0% | |
| AAAAAGGCAATCCTCATCCT+AGG | - | chr5.4:66893477-66893496 | MS.gene90607:CDS | 40.0% | |
| AAATATGGCGGTTACCACAA+TGG | - | chr5.4:66893976-66893995 | MS.gene90607:intron | 40.0% | |
| AACTTCCTCTGATGCCAAAA+AGG | - | chr5.4:66893545-66893564 | MS.gene90607:CDS | 40.0% | |
| ACTTCCTCTGATGCCAAAAA+GGG | - | chr5.4:66893546-66893565 | MS.gene90607:CDS | 40.0% | |
| ATTGTGGTAACCGCCATATT+TGG | + | chr5.4:66893977-66893996 | None:intergenic | 40.0% | |
| GTAAACGATGCCAAATATGG+CGG | - | chr5.4:66893964-66893983 | MS.gene90607:intron | 40.0% | |
| GTAAACGATGCCAAATATGG+TGG | - | chr5.4:66893964-66893983 | MS.gene90607:intron | 40.0% | |
| ! | AGAGTAGAACTCACCCTTTT+TGG | + | chr5.4:66893562-66893581 | None:intergenic | 40.0% |
| !! | AATGGTGGTGGACATTACTA+TGG | - | chr5.4:66894015-66894034 | MS.gene90607:intron | 40.0% |
| AACGATGCCAAATATGGTGG+TGG | - | chr5.4:66894165-66894184 | MS.gene90607:intron | 45.0% | |
| AATGGCCGTGGCAATAGTAA+TGG | + | chr5.4:66894275-66894294 | None:intergenic | 45.0% | |
| AGTAATGTCCACCACCATTG+TGG | + | chr5.4:66894014-66894033 | None:intergenic | 45.0% | |
| ATTCAGCATGTGAGCAGCAA+TGG | + | chr5.4:66894293-66894312 | None:intergenic | 45.0% | |
| CAAGAAGAACCATTGCCAAG+AGG | + | chr5.4:66893503-66893522 | None:intergenic | 45.0% | |
| GGTAATTACCACAATGGTGG+TGG | - | chr5.4:66894003-66894022 | MS.gene90607:intron | 45.0% | |
| GGTAATTACCACCACCATTG+TGG | + | chr5.4:66893993-66894012 | None:intergenic | 45.0% | |
| GGTGGTAATTACCACAATGG+TGG | - | chr5.4:66894000-66894019 | MS.gene90607:intron | 45.0% | |
| GGTTCTTCTTGTGTCAGCTA+GGG | - | chr5.4:66893512-66893531 | MS.gene90607:CDS | 45.0% | |
| GGTTGCCATTACTATTGCCA+CGG | - | chr5.4:66894267-66894286 | MS.gene90607:CDS | 45.0% | |
| TACTATGGAGGTGGTTCTCA+CGG | - | chr5.4:66894030-66894049 | MS.gene90607:intron | 45.0% | |
| TGGTTCTTCTTGTGTCAGCT+AGG | - | chr5.4:66893511-66893530 | MS.gene90607:CDS | 45.0% | |
| ! | AGTCTTTTCCTCAGCTTGCA+CGG | + | chr5.4:66894136-66894155 | None:intergenic | 45.0% |
| ! | GGTGGTGGTAATTACCACAA+TGG | - | chr5.4:66893997-66894016 | MS.gene90607:intron | 45.0% |
| !! | AGTTTTGTCCTCAGCTTGCA+CGG | + | chr5.4:66894322-66894341 | None:intergenic | 45.0% |
| AATCCTCATCCTAGGCCTCT+TGG | - | chr5.4:66893485-66893504 | MS.gene90607:CDS | 50.0% | |
| AATGGCCGTGGCAATAGTGA+TGG | + | chr5.4:66894089-66894108 | None:intergenic | 50.0% | |
| ACTCGGCATATGAGCAGCAA+TGG | + | chr5.4:66894107-66894126 | None:intergenic | 50.0% | |
| AGCTTGCACGGCAACAAACT+CGG | + | chr5.4:66894124-66894143 | None:intergenic | 50.0% | |
| GGTGGACATTACTATGGAGG+TGG | - | chr5.4:66894021-66894040 | MS.gene90607:intron | 50.0% | |
| GGTTGCCATCACTATTGCCA+CGG | - | chr5.4:66894081-66894100 | MS.gene90607:intron | 50.0% | |
| TATGGAGGTGGTTCTCACCA+CGG | - | chr5.4:66894216-66894235 | MS.gene90607:intron | 50.0% | |
| TATGGCGGTTACCACAATGG+TGG | - | chr5.4:66893979-66893998 | MS.gene90607:intron | 50.0% | |
| ! | ATTTGTTGCCGTGCAAGCTG+AGG | - | chr5.4:66894311-66894330 | MS.gene90607:CDS | 50.0% |
| !! | CTCACCCTTTTTGGCATCAG+AGG | + | chr5.4:66893553-66893572 | None:intergenic | 50.0% |
| !! | GGTGGTGGACATTACTATGG+AGG | - | chr5.4:66894018-66894037 | MS.gene90607:intron | 50.0% |
| AATGGCAACCACCTCCACCA+TGG | + | chr5.4:66894257-66894276 | None:intergenic | 55.0% | |
| CATATGAGCAGCAATGGCCG+TGG | + | chr5.4:66894101-66894120 | None:intergenic | 55.0% | |
| CATCCTAGGCCTCTTGGCAA+TGG | - | chr5.4:66893491-66893510 | MS.gene90607:CDS | 55.0% | |
| GAACCATTGCCAAGAGGCCT+AGG | + | chr5.4:66893497-66893516 | None:intergenic | 55.0% | |
| TATGGAGGTGGTTCTCACGG+TGG | - | chr5.4:66894033-66894052 | MS.gene90607:intron | 55.0% | |
| TTGCCAAGAGGCCTAGGATG+AGG | + | chr5.4:66893491-66893510 | None:intergenic | 55.0% | |
| ! | GTTTGTTGCCGTGCAAGCTG+AGG | - | chr5.4:66894125-66894144 | MS.gene90607:intron | 55.0% |
| CATGTGAGCAGCAATGGCCG+TGG | + | chr5.4:66894287-66894306 | None:intergenic | 60.0% | |
| GGAGGTGGTTCTCACCATGG+TGG | - | chr5.4:66894240-66894259 | MS.gene90607:intron | 60.0% | |
| GGCGGTTACCACAATGGTGG+TGG | - | chr5.4:66893982-66894001 | MS.gene90607:intron | 60.0% | |
| GGTGGAGGTGGTTCTCACCA+TGG | - | chr5.4:66894237-66894256 | MS.gene90607:intron | 60.0% | |
| GGTGGTTCTCACCATGGTGG+AGG | - | chr5.4:66894243-66894262 | MS.gene90607:intron | 60.0% | |
| ! | GGTTCTCACCATGGTGGAGG+TGG | - | chr5.4:66894246-66894265 | MS.gene90607:intron | 60.0% |
| GATGGCAACCACCTCCACCG+TGG | + | chr5.4:66894071-66894090 | None:intergenic | 65.0% | |
| GCAACCACCTCCACCGTGGT+GGG | + | chr5.4:66894067-66894086 | None:intergenic | 65.0% | |
| GGAGGTGGTTCTCACCACGG+TGG | - | chr5.4:66894219-66894238 | MS.gene90607:intron | 65.0% | |
| GGAGGTGGTTCTCACGGTGG+AGG | - | chr5.4:66894036-66894055 | MS.gene90607:intron | 65.0% | |
| GGTGAGAACCACCTCCACCG+TGG | + | chr5.4:66894236-66894255 | None:intergenic | 65.0% | |
| GGTGGAGGTGGTTCCCACCA+CGG | - | chr5.4:66894051-66894070 | MS.gene90607:intron | 65.0% | |
| GGTGGTTCTCACCACGGTGG+AGG | - | chr5.4:66894222-66894241 | MS.gene90607:intron | 65.0% | |
| GGTGGTTCTCACGGTGGAGG+TGG | - | chr5.4:66894039-66894058 | MS.gene90607:intron | 65.0% | |
| GGTTCTCACCACGGTGGAGG+TGG | - | chr5.4:66894225-66894244 | MS.gene90607:intron | 65.0% | |
| GGAGGTGGTTCCCACCACGG+TGG | - | chr5.4:66894054-66894073 | MS.gene90607:intron | 70.0% | |
| GGCAACCACCTCCACCGTGG+TGG | + | chr5.4:66894068-66894087 | None:intergenic | 70.0% | |
| GGTGGTTCCCACCACGGTGG+AGG | - | chr5.4:66894057-66894076 | MS.gene90607:intron | 70.0% | |
| GGTTCCCACCACGGTGGAGG+TGG | - | chr5.4:66894060-66894079 | MS.gene90607:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 66893468 | 66894350 | 66893468 | ID=MS.gene90607 |
| chr5.4 | mRNA | 66893468 | 66894350 | 66893468 | ID=MS.gene90607.t1;Parent=MS.gene90607 |
| chr5.4 | exon | 66894251 | 66894350 | 66894251 | ID=MS.gene90607.t1.exon1;Parent=MS.gene90607.t1 |
| chr5.4 | CDS | 66894251 | 66894350 | 66894251 | ID=cds.MS.gene90607.t1;Parent=MS.gene90607.t1 |
| chr5.4 | exon | 66893468 | 66893877 | 66893468 | ID=MS.gene90607.t1.exon2;Parent=MS.gene90607.t1 |
| chr5.4 | CDS | 66893468 | 66893877 | 66893468 | ID=cds.MS.gene90607.t1;Parent=MS.gene90607.t1 |
| Gene Sequence |
| Protein sequence |
