AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90905

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90905.t1 MTR_1g116957 97.771 1032 18 3 1 1032 1 1027 0.0 2067
MS.gene90905.t1 MTR_7g021500 79.614 1035 196 5 4 1032 6 1031 0.0 1674
MS.gene90905.t1 MTR_7g021500 79.903 1035 194 5 4 1032 6 1032 0.0 1669
MS.gene90905.t1 MTR_7g107210 70.229 131 21 2 861 991 62 174 3.26e-35 132
MS.gene90905.t1 MTR_7g107210 88.235 51 4 1 732 780 1 51 3.95e-22 95.1
MS.gene90905.t1 MTR_7g107220 85.714 49 7 0 605 653 12 60 1.87e-20 86.3
MS.gene90905.t1 MTR_4g123690 22.864 433 267 15 1 394 1 405 5.13e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90905.t1 AT2G31660 70.270 1036 289 6 5 1030 8 1034 0.0 1479
MS.gene90905.t1 AT3G59020 69.826 1034 291 6 4 1030 7 1026 0.0 1429
MS.gene90905.t1 AT3G59020 69.662 1035 292 6 4 1030 7 1027 0.0 1422

Find 220 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATATAAATTACCAAATTT+TGG 0.144050 1.2:-86747241 None:intergenic
TGTTAACATGCTACGCATTA+TGG 0.175977 1.2:-86747521 None:intergenic
GCAGTCCTGTTGGCCATCTT+AGG 0.178159 1.2:+86745663 MS.gene90905:CDS
GCATTATGCGGGGAAGATTT+TGG 0.199329 1.2:+86744792 MS.gene90905:CDS
ACGCTCAAGTTCAGATTTAT+AGG 0.205041 1.2:-86745609 None:intergenic
CATCAGAAATCTTCAATCTT+TGG 0.212439 1.2:+86749158 MS.gene90905:CDS
CAACAAAGGCTGCTGCATTT+TGG 0.228833 1.2:+86747350 MS.gene90905:intron
TCTTGACTTCTTGCAGCATT+TGG 0.257051 1.2:-86749183 None:intergenic
AGTTTATTCTGCAGATATTA+TGG 0.266161 1.2:+86748242 MS.gene90905:intron
CTTCTCCCTTCGCTCCTTGA+TGG 0.266476 1.2:+86747032 MS.gene90905:CDS
GATGGAGCTCTTCTTGCTAT+TGG 0.266710 1.2:+86745554 MS.gene90905:CDS
ACTTGGGCTATGCCAAAATT+TGG 0.272370 1.2:+86747229 MS.gene90905:CDS
CCTTGGTTTAACATCCTTGC+TGG 0.273246 1.2:+86749399 MS.gene90905:CDS
CCACCAGTCCTCTGCAGTTT+AGG 0.276869 1.2:-86748569 None:intergenic
TCTCAGATCAGAACAATTTC+CGG 0.301077 1.2:+86746798 MS.gene90905:CDS
TCGCCGATAAAGACTTTGTT+AGG 0.308150 1.2:+86743920 MS.gene90905:CDS
AGCTTATCTGTAAAATATTC+TGG 0.310967 1.2:+86744352 MS.gene90905:CDS
TGCAGATATTATGGTTCCTT+TGG 0.312879 1.2:+86748251 MS.gene90905:intron
AAAACTTCATTGCTAAGAAT+TGG 0.314964 1.2:+86743872 MS.gene90905:CDS
GATAAGAGAACTCAAGTGTT+TGG 0.315709 1.2:-86750980 None:intergenic
GGCAAGAAAGTTTGCTGCCT+TGG 0.320902 1.2:+86749382 MS.gene90905:CDS
AGAAAATGACGACGATGATA+TGG 0.321952 1.2:+86749697 MS.gene90905:CDS
CTCTTCTCGTCTCAGCATGC+TGG 0.322424 1.2:-86751025 None:intergenic
GTTCTTTGCATGTAATGAAA+TGG 0.338050 1.2:-86748299 None:intergenic
ATTCCTAACAAAGTCTTTAT+CGG 0.340148 1.2:-86743923 None:intergenic
AAAGGATCCACTTCATCAAT+TGG 0.341738 1.2:-86750779 None:intergenic
CAGTAGAATACAGGTCTTAT+AGG 0.342847 1.2:+86745525 MS.gene90905:CDS
GCCTTTCTTTCATTCGGATT+CGG 0.343757 1.2:-86743721 None:intergenic
AAACAGGCAGCGTGGGTTGC+AGG 0.351039 1.2:+86746755 MS.gene90905:intron
ATGGAGCTCTTCTTGCTATT+GGG 0.353649 1.2:+86745555 MS.gene90905:CDS
AAGAATGCGGAGAACGAATA+AGG 0.358824 1.2:-86744187 None:intergenic
ACTGTCCCCATCTTCTTGAT+TGG 0.360820 1.2:+86744121 MS.gene90905:CDS
ATGATAATGATCAAAAGCTT+TGG 0.361240 1.2:+86745164 MS.gene90905:CDS
CGCTCAAGTTCAGATTTATA+GGG 0.366117 1.2:-86745608 None:intergenic
AAGACAATTTCAATCAGCTT+TGG 0.366324 1.2:-86748546 None:intergenic
CACTGGACATGTGGAACCTT+TGG 0.368126 1.2:+86747816 MS.gene90905:CDS
TCCAATAGGAAACGTCATTC+AGG 0.373563 1.2:+86750702 MS.gene90905:intron
CAACACGTCTTTCCCGAGTT+TGG 0.382141 1.2:+86745641 MS.gene90905:CDS
ATCAAAATGTATTCAGTGCT+TGG 0.383720 1.2:+86744493 MS.gene90905:CDS
CAGTGGTTCTGACAGGGAAA+TGG 0.388816 1.2:+86749754 MS.gene90905:CDS
CAGTCCTGTTGGCCATCTTA+GGG 0.388883 1.2:+86745664 MS.gene90905:CDS
GGGACAGTGGTCGGGATAAT+CGG 0.391844 1.2:-86744106 None:intergenic
ATTGAACGTTTGCATAGAAC+TGG 0.394636 1.2:+86748624 MS.gene90905:CDS
GAATGGATGGATTAGGAATC+TGG 0.395918 1.2:-86744311 None:intergenic
CCCCATGATTTGCGAAGATC+AGG 0.398780 1.2:-86744576 None:intergenic
AATTCAAATCAGACGAAGAT+AGG 0.399839 1.2:+86744223 MS.gene90905:CDS
TGATAATGATCAAAAGCTTT+GGG 0.400167 1.2:+86745165 MS.gene90905:CDS
ATAAGAGAACTCAAGTGTTT+GGG 0.402571 1.2:-86750979 None:intergenic
GTTAACATGCTACGCATTAT+GGG 0.406544 1.2:-86747520 None:intergenic
AAGGATCCACTTCATCAATT+GGG 0.407208 1.2:-86750778 None:intergenic
TAAAAGAGTTGGAAGATGAT+TGG 0.409023 1.2:-86743779 None:intergenic
CTGACTATCAACAGTGTTTG+TGG 0.410520 1.2:+86748322 MS.gene90905:CDS
CCGTGCCACTCTTGATCTCC+TGG 0.410642 1.2:+86749468 MS.gene90905:CDS
AAGATCCACATGAGTATGTT+AGG 0.411092 1.2:+86745191 MS.gene90905:CDS
TCAGCAGCCTTTCTTTCATT+CGG 0.412223 1.2:-86743727 None:intergenic
CTTAGCATATTGCAAAAGTT+AGG 0.417195 1.2:+86749130 MS.gene90905:CDS
ATCCTGATCTTCGCAAATCA+TGG 0.418182 1.2:+86744574 MS.gene90905:CDS
GAGACTATTGTGGACAAGTT+TGG 0.418235 1.2:+86747185 MS.gene90905:CDS
GCAACTTGAATGGATGGATT+AGG 0.420585 1.2:-86744318 None:intergenic
CACAAACACTGTTGATAGTC+AGG 0.421066 1.2:-86748321 None:intergenic
TTGCGAAGATCAGGATCAAC+AGG 0.425592 1.2:-86744567 None:intergenic
TGAAGACCTTGTGTTTACTT+TGG 0.426103 1.2:+86747163 MS.gene90905:CDS
CTTTGGACAATTATATTTCA+AGG 0.426833 1.2:+86748268 MS.gene90905:CDS
GGCTGACCTTCCAAGGGAAC+AGG 0.427511 1.2:-86744546 None:intergenic
TGGTTAGATATTGAAGAAGT+AGG 0.428313 1.2:-86744879 None:intergenic
TGATCTCCTGGTTGCATACA+AGG 0.431116 1.2:+86749480 MS.gene90905:CDS
GCAGAGGACTGGTGGACCAC+TGG 0.433625 1.2:+86748577 MS.gene90905:CDS
TGGCATAGCCCAAGTGCATA+AGG 0.439443 1.2:-86747221 None:intergenic
GAAGATGATTGGGTGCATAT+TGG 0.439602 1.2:-86743768 None:intergenic
AGAACTCAAGTGTTTGGGTC+AGG 0.439926 1.2:-86750974 None:intergenic
TTCTTTGCATGTAATGAAAT+GGG 0.441132 1.2:-86748298 None:intergenic
CAACACATTGCAATGCCTTC+CGG 0.443732 1.2:-86746817 None:intergenic
GCATACAAGGATCAAGTTGC+AGG 0.446246 1.2:+86749493 MS.gene90905:CDS
TCAATGCGGACAGGTAGTTC+AGG 0.449994 1.2:-86746857 None:intergenic
GTTTGATCCAATCAAGAAGA+TGG 0.450885 1.2:-86744128 None:intergenic
GTTCTTCAAACTTATGAATG+AGG 0.452917 1.2:+86747133 MS.gene90905:CDS
TTCTTCAATATCTAACCAAC+AGG 0.453303 1.2:+86744884 MS.gene90905:CDS
CCCGAGTTTGGCAGTCCTGT+TGG 0.454925 1.2:+86745653 MS.gene90905:CDS
CAATTGCCAGGAGAGGCTTT+AGG 0.466553 1.2:+86749436 MS.gene90905:CDS
GAACCTTTGGCCATTGATGA+TGG 0.467627 1.2:+86747829 MS.gene90905:CDS
TCTTATCAAGCTCTTGCAAA+TGG 0.467812 1.2:+86750996 MS.gene90905:CDS
AGTGGTTCTGACAGGGAAAT+GGG 0.468849 1.2:+86749755 MS.gene90905:CDS
ATTGGAGAGGCCTGTTCCCT+TGG 0.469011 1.2:+86744536 MS.gene90905:CDS
ATGGGAACTGATGCTGAAGA+TGG 0.471480 1.2:+86749773 MS.gene90905:CDS
TCCTGATCTTCGCAAATCAT+GGG 0.471937 1.2:+86744575 MS.gene90905:CDS
TGTCTCGTGAATAATGTGAT+AGG 0.472814 1.2:-86744253 None:intergenic
CACATGTCCAGTGATATTGT+TGG 0.473110 1.2:-86747806 None:intergenic
TCTCCTGGCAATTGATCAGC+TGG 0.473397 1.2:-86749427 None:intergenic
TTGAAGACTTGTATAGTCCT+AGG 0.475338 1.2:+86745318 MS.gene90905:CDS
CTTCCAGCTGATCAATTGCC+AGG 0.475735 1.2:+86749424 MS.gene90905:CDS
TCATCTGAGTCCTCATCATC+GGG 0.483411 1.2:-86750725 None:intergenic
ACGATGATTTGTAAAAGAGT+TGG 0.483438 1.2:-86743790 None:intergenic
AGGATGACAGTGGTTCTGAC+AGG 0.484366 1.2:+86749747 MS.gene90905:CDS
GTTAAATATATTGAGAAGGT+GGG 0.484925 1.2:-86744280 None:intergenic
AAATCCACGGAGGCAGTCCT+AGG 0.485246 1.2:-86745335 None:intergenic
GTCATCTGAGTCCTCATCAT+CGG 0.486522 1.2:-86750726 None:intergenic
ATGTTTCACAAGCATTATGC+GGG 0.488084 1.2:+86744781 MS.gene90905:CDS
CTATAAGACCTGTATTCTAC+TGG 0.488098 1.2:-86745524 None:intergenic
ACTTAGAAAATTGACTGATC+AGG 0.489306 1.2:+86749817 MS.gene90905:CDS
TTGGCCCTAAGATGGCCAAC+AGG 0.489970 1.2:-86745668 None:intergenic
TGACATTCAACAGATTTAAG+TGG 0.490324 1.2:-86744819 None:intergenic
AGAAGATGGGGACAGTGGTC+GGG 0.491280 1.2:-86744114 None:intergenic
TTTCTCTCCAACAATATCAC+TGG 0.494642 1.2:+86747799 MS.gene90905:CDS
GAAGTAGTTGTTATACTTGA+AGG 0.494802 1.2:+86743688 MS.gene90905:CDS
CAAGAGTGGCACGGAATACC+CGG 0.497487 1.2:-86749459 None:intergenic
TGAGATTGAGAAGGAAAAGT+CGG 0.497844 1.2:+86751049 MS.gene90905:CDS
ATGAAGCACCAGTAGAATAC+AGG 0.501247 1.2:+86745516 MS.gene90905:CDS
TGATGGAAGCACTATCAGAT+TGG 0.503592 1.2:+86747846 MS.gene90905:CDS
GATGGAAGCACTATCAGATT+GGG 0.504868 1.2:+86747847 MS.gene90905:CDS
GCCAACAGGACTGCCAAACT+CGG 0.505683 1.2:-86745654 None:intergenic
ATCAACAGGCTGACCTTCCA+AGG 0.505833 1.2:-86744553 None:intergenic
GGACAAGTTTGGTGAAGAAA+TGG 0.506738 1.2:+86747196 MS.gene90905:CDS
ACCCGGCCTAAAGCCTCTCC+TGG 0.506757 1.2:-86749442 None:intergenic
AAAAGAGTTGGAAGATGATT+GGG 0.509158 1.2:-86743778 None:intergenic
TTCGCAAATCATGGGGTTGG+TGG 0.509717 1.2:+86744583 MS.gene90905:CDS
GAAATGGCACCTTATGCACT+TGG 0.509924 1.2:+86747212 MS.gene90905:CDS
AGTAGGTTGATAACTCTGTC+AGG 0.510634 1.2:-86744862 None:intergenic
ACAGTCCCCAATTGATGAAG+TGG 0.510949 1.2:+86750772 MS.gene90905:CDS
GCCAGGAGAGGCTTTAGGCC+GGG 0.512244 1.2:+86749441 MS.gene90905:CDS
TTTGGACAATTATATTTCAA+GGG 0.512445 1.2:+86748269 MS.gene90905:CDS
AAAGGCTGCTGAACAAAGGT+TGG 0.513295 1.2:+86743738 MS.gene90905:CDS
CGCTGATGATTCAAGTTCAA+TGG 0.515595 1.2:+86747405 MS.gene90905:CDS
CTTTGCAGAAGCTGCAGAAG+AGG 0.515709 1.2:+86749661 MS.gene90905:intron
AAGAAGATGGGGACAGTGGT+CGG 0.516125 1.2:-86744115 None:intergenic
TGTTAAATATATTGAGAAGG+TGG 0.516144 1.2:-86744281 None:intergenic
TGCCAGGAGAGGCTTTAGGC+CGG 0.518509 1.2:+86749440 MS.gene90905:CDS
TCTTCTCGTCTCAGCATGCT+GGG 0.520955 1.2:-86751024 None:intergenic
AGATCAGCAACTTGAATGGA+TGG 0.521481 1.2:-86744324 None:intergenic
GTGAGTTGGTTCGAAAGCGC+AGG 0.523922 1.2:+86745363 MS.gene90905:CDS
TGTATACTGTGCTGCAGCCT+CGG 0.524758 1.2:+86745095 MS.gene90905:CDS
TCAATTGTAATTCTGAGATA+CGG 0.526551 1.2:-86748606 None:intergenic
CTTCCTAAACTGCAGAGGAC+TGG 0.526734 1.2:+86748566 MS.gene90905:CDS
TTTGATCCAATCAAGAAGAT+GGG 0.528330 1.2:-86744127 None:intergenic
CGCATTATGGGAAGCAAGGT+AGG 0.531290 1.2:-86747508 None:intergenic
GTCATTCAGGCCCGATGATG+AGG 0.532142 1.2:+86750715 MS.gene90905:CDS
GAGAGGCCTGTTCCCTTGGA+AGG 0.534183 1.2:+86744540 MS.gene90905:CDS
TCCGAATCCGAATGAAAGAA+AGG 0.536209 1.2:+86743720 MS.gene90905:CDS
ACCGTACCTTGGCCCTAAGA+TGG 0.536280 1.2:-86745676 None:intergenic
CTGTTGAATGTCATTCGAGC+AGG 0.539977 1.2:+86744829 MS.gene90905:CDS
GAAATGTCTTCTCATTCAAG+TGG 0.539995 1.2:+86748659 MS.gene90905:CDS
TGTTGGCCATCTTAGGGCCA+AGG 0.540483 1.2:+86745670 MS.gene90905:CDS
AGCTGATCAATTGCCAGGAG+AGG 0.544550 1.2:+86749429 MS.gene90905:CDS
ATCTTCGCAAATCATGGGGT+TGG 0.544792 1.2:+86744580 MS.gene90905:CDS
GATAGTGCTTCCATCATCAA+TGG 0.545002 1.2:-86747839 None:intergenic
CAGAGGACTGGTGGACCACT+GGG 0.550023 1.2:+86748578 MS.gene90905:CDS
TGGGAACTGATGCTGAAGAT+GGG 0.553757 1.2:+86749774 MS.gene90905:CDS
CAACAATATCACTGGACATG+TGG 0.554151 1.2:+86747807 MS.gene90905:CDS
TCGTGAATAATGTGATAGGC+AGG 0.555259 1.2:-86744249 None:intergenic
AGGTCTTATAGGCAGAAAGA+TGG 0.556191 1.2:+86745536 MS.gene90905:CDS
CTCAAAAGAGAAGGAAGTTG+AGG 0.557170 1.2:-86743964 None:intergenic
GTTTACTTTGGAGACTATTG+TGG 0.557940 1.2:+86747175 MS.gene90905:CDS
ATAGGTACGTACATATATAG+AGG 0.560975 1.2:-86744376 None:intergenic
TGCAGAAGCTGCAGAAGAGG+AGG 0.562224 1.2:+86749664 MS.gene90905:intron
CACGTACCATCAAGGAGCGA+AGG 0.566318 1.2:-86747038 None:intergenic
GCTGCGAAACCCTGAAAACA+AGG 0.567003 1.2:+86744747 MS.gene90905:CDS
GAGTATGTTAGGAAAGGCTA+TGG 0.572371 1.2:+86745202 MS.gene90905:CDS
ATCAGCTGGAAGTGCCAGCA+AGG 0.574149 1.2:-86749413 None:intergenic
GATGAGATCAGCAACTTGAA+TGG 0.574179 1.2:-86744328 None:intergenic
CAGCATGCTGAGACGAGAAG+AGG 0.576433 1.2:+86751026 MS.gene90905:CDS
GAATGGCGGCAACTTGTCTG+AGG 0.576913 1.2:-86743843 None:intergenic
TTAAATATATTGAGAAGGTG+GGG 0.581029 1.2:-86744279 None:intergenic
CCTAAACTGCAGAGGACTGG+TGG 0.581251 1.2:+86748569 MS.gene90905:CDS
GAGAAGAGGTGAGATTGAGA+AGG 0.581346 1.2:+86751040 MS.gene90905:CDS
TTGAATGTCATTCGAGCAGG+TGG 0.583758 1.2:+86744832 MS.gene90905:CDS
TAAGGGAATACCTTGACCAT+CGG 0.584319 1.2:-86747545 None:intergenic
ATAGCATACCTCAAAAGAGA+AGG 0.586840 1.2:-86743973 None:intergenic
TTGGACAATTATATTTCAAG+GGG 0.589802 1.2:+86748270 MS.gene90905:CDS
CCACATGAGTATGTTAGGAA+AGG 0.589934 1.2:+86745196 MS.gene90905:CDS
AAAACAGAATCAATGCGGAC+AGG 0.593718 1.2:-86746866 None:intergenic
TATCATTGAAGCACATGAGA+GGG 0.594402 1.2:-86745147 None:intergenic
AATGTTTCACAAGCATTATG+CGG 0.595466 1.2:+86744780 MS.gene90905:CDS
TCCAAAAGCTTAAGAATCTG+TGG 0.596418 1.2:-86751087 None:intergenic
AAAGAAAGGCTGCTGAACAA+AGG 0.597576 1.2:+86743734 MS.gene90905:CDS
TCAAGTTCAATGGCTGCAGT+TGG 0.600507 1.2:+86747415 MS.gene90905:CDS
CCAACAGGACTGCCAAACTC+GGG 0.601191 1.2:-86745653 None:intergenic
AGGATCCACTTCATCAATTG+GGG 0.601464 1.2:-86750777 None:intergenic
CATATTTAATAGAAAGAATG+CGG 0.602450 1.2:-86744200 None:intergenic
ATGTTAACAACCGATGGTCA+AGG 0.603700 1.2:+86747535 MS.gene90905:CDS
CTTGATCCTTGTATGCAACC+AGG 0.604622 1.2:-86749486 None:intergenic
GTGTGTTAAATATATTGAGA+AGG 0.607757 1.2:-86744284 None:intergenic
TCTGATCTGAGAAGCTAATG+TGG 0.613687 1.2:-86746787 None:intergenic
GGATGACAGTGGTTCTGACA+GGG 0.618263 1.2:+86749748 MS.gene90905:CDS
TTCTAAACAGACTCTACACT+CGG 0.620641 1.2:+86744631 MS.gene90905:CDS
GCTACGCATTATGGGAAGCA+AGG 0.622515 1.2:-86747512 None:intergenic
TGAGTTGGTTCGAAAGCGCA+GGG 0.626418 1.2:+86745364 MS.gene90905:CDS
TAGTCCTAGGACTGCCTCCG+TGG 0.626651 1.2:+86745331 MS.gene90905:CDS
AAATGGCACCTTATGCACTT+GGG 0.628408 1.2:+86747213 MS.gene90905:CDS
CGTAGCATGTTAACAACCGA+TGG 0.630518 1.2:+86747529 MS.gene90905:CDS
CTTGAAATATAATTGTCCAA+AGG 0.630964 1.2:-86748267 None:intergenic
AATGGCGGCAACTTGTCTGA+GGG 0.630984 1.2:-86743842 None:intergenic
CAACTCACTCATAAAATCCA+CGG 0.633012 1.2:-86745348 None:intergenic
AAAAGAAGAACGTCAAGCCG+AGG 0.634422 1.2:-86745112 None:intergenic
CTGCAAGAAGTCAAGAAGAG+TGG 0.634459 1.2:+86749190 MS.gene90905:CDS
TTATCATTGAAGCACATGAG+AGG 0.634688 1.2:-86745148 None:intergenic
ATTGTCTTCCTAAACTGCAG+AGG 0.634847 1.2:+86748561 MS.gene90905:CDS
CTTCCATCATCAATGGCCAA+AGG 0.636876 1.2:-86747832 None:intergenic
GAAAGAGATTGAGTGAGCAG+TGG 0.640985 1.2:-86743899 None:intergenic
AAATGTATTCAGTGCTTGGA+TGG 0.641307 1.2:+86744497 MS.gene90905:CDS
ACGTACCATCAAGGAGCGAA+GGG 0.642973 1.2:-86747037 None:intergenic
CCTGATCTTCGCAAATCATG+GGG 0.645502 1.2:+86744576 MS.gene90905:CDS
CCTTTCCTAACATACTCATG+TGG 0.647215 1.2:-86745196 None:intergenic
TCAAACTGATGATGAAGATG+AGG 0.647870 1.2:+86749727 MS.gene90905:CDS
CTGATCTGAGAAGCTAATGT+GGG 0.648144 1.2:-86746786 None:intergenic
TTGATCCAATCAAGAAGATG+GGG 0.650413 1.2:-86744126 None:intergenic
CCAGGAGATCAAGAGTGGCA+CGG 0.650577 1.2:-86749468 None:intergenic
TGCAACCAGGAGATCAAGAG+TGG 0.652472 1.2:-86749473 None:intergenic
TGCAATCATCAGATCCAATG+AGG 0.656544 1.2:+86750943 MS.gene90905:CDS
AATCAAGAAGATGGGGACAG+TGG 0.659255 1.2:-86744119 None:intergenic
GCATTGCAATGTGTTGTCTG+TGG 0.661281 1.2:+86746824 MS.gene90905:CDS
AGATCAGAACAATTTCCGGA+AGG 0.668438 1.2:+86746802 MS.gene90905:CDS
GGGAACTGATGCTGAAGATG+GGG 0.669290 1.2:+86749775 MS.gene90905:CDS
TCAACAGGCTGACCTTCCAA+GGG 0.680227 1.2:-86744552 None:intergenic
TAGTCTCCAAAGTAAACACA+AGG 0.681795 1.2:-86747169 None:intergenic
AATGACGACGATGATATGGA+CGG 0.695430 1.2:+86749701 MS.gene90905:CDS
CAATATGCTAAGAGTCAATG+TGG 0.695584 1.2:-86749119 None:intergenic
TGTTTCACAAGCATTATGCG+GGG 0.696183 1.2:+86744782 MS.gene90905:CDS
TGAGATACGGCTCAACCCAG+TGG 0.705549 1.2:-86748593 None:intergenic
CCAGCAAGGATGTTAAACCA+AGG 0.712970 1.2:-86749399 None:intergenic
GATGAAGATGAGGATGACAG+TGG 0.770603 1.2:+86749737 MS.gene90905:CDS
CTCACTCATAAAATCCACGG+AGG 0.783942 1.2:-86745345 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr1.2 gene 86743682 86751100 86743682 ID=MS.gene90905
chr1.2 mRNA 86743682 86751100 86743682 ID=MS.gene90905.t1;Parent=MS.gene90905
chr1.2 exon 86743682 86743986 86743682 ID=MS.gene90905.t1.exon1;Parent=MS.gene90905.t1
chr1.2 CDS 86743682 86743986 86743682 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86744068 86744386 86744068 ID=MS.gene90905.t1.exon2;Parent=MS.gene90905.t1
chr1.2 CDS 86744068 86744386 86744068 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86744468 86744652 86744468 ID=MS.gene90905.t1.exon3;Parent=MS.gene90905.t1
chr1.2 CDS 86744468 86744652 86744468 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86744732 86744905 86744732 ID=MS.gene90905.t1.exon4;Parent=MS.gene90905.t1
chr1.2 CDS 86744732 86744905 86744732 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86745078 86745223 86745078 ID=MS.gene90905.t1.exon5;Parent=MS.gene90905.t1
chr1.2 CDS 86745078 86745223 86745078 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86745312 86745435 86745312 ID=MS.gene90905.t1.exon6;Parent=MS.gene90905.t1
chr1.2 CDS 86745312 86745435 86745312 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86745511 86745691 86745511 ID=MS.gene90905.t1.exon7;Parent=MS.gene90905.t1
chr1.2 CDS 86745511 86745691 86745511 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86746761 86746920 86746761 ID=MS.gene90905.t1.exon8;Parent=MS.gene90905.t1
chr1.2 CDS 86746761 86746920 86746761 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86747012 86747053 86747012 ID=MS.gene90905.t1.exon9;Parent=MS.gene90905.t1
chr1.2 CDS 86747012 86747053 86747012 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86747132 86747250 86747132 ID=MS.gene90905.t1.exon10;Parent=MS.gene90905.t1
chr1.2 CDS 86747132 86747250 86747132 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86747358 86747556 86747358 ID=MS.gene90905.t1.exon11;Parent=MS.gene90905.t1
chr1.2 CDS 86747358 86747556 86747358 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86747756 86747887 86747756 ID=MS.gene90905.t1.exon12;Parent=MS.gene90905.t1
chr1.2 CDS 86747756 86747887 86747756 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86748256 86748359 86748256 ID=MS.gene90905.t1.exon13;Parent=MS.gene90905.t1
chr1.2 CDS 86748256 86748359 86748256 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86748501 86748680 86748501 ID=MS.gene90905.t1.exon14;Parent=MS.gene90905.t1
chr1.2 CDS 86748501 86748680 86748501 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86749094 86749233 86749094 ID=MS.gene90905.t1.exon15;Parent=MS.gene90905.t1
chr1.2 CDS 86749094 86749233 86749094 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86749375 86749514 86749375 ID=MS.gene90905.t1.exon16;Parent=MS.gene90905.t1
chr1.2 CDS 86749375 86749514 86749375 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86749669 86749838 86749669 ID=MS.gene90905.t1.exon17;Parent=MS.gene90905.t1
chr1.2 CDS 86749669 86749838 86749669 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86750710 86750827 86750710 ID=MS.gene90905.t1.exon18;Parent=MS.gene90905.t1
chr1.2 CDS 86750710 86750827 86750710 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
chr1.2 exon 86750940 86751100 86750940 ID=MS.gene90905.t1.exon19;Parent=MS.gene90905.t1
chr1.2 CDS 86750940 86751100 86750940 ID=cds.MS.gene90905.t1;Parent=MS.gene90905.t1
Gene Sequence

>MS.gene90905

ATGGATGAAGTAGTTGTTATACTTGAAGGAACTCTGAGTCCGAATCCGAATGAAAGAAAGGCTGCTGAACAAAGGTTGGATGAGATCCAATATGCACCCAATCATCTTCCAACTCTTTTACAAATCATCGTTCAATCAGACGATTCTGTCTCTGATATTTCCCTCAGACAAGTTGCCGCCATTCATTTCAAAAACTTCATTGCTAAGAATTGGTCTTCCACTGCTCACTCAATCTCTTTCGCCGATAAAGACTTTGTTAGGAATCACATTCTCCTCTTTTTACCTCAACTTCCTTCTCTTTTGAGGTATGCTATACTATACTCCATTCTCCATTGCACTCGCACTCGCACTCTACCTGTTTGTAATAATTCCCATTCCATTTTCAGGGTTCAACTTGGTGAGTGCCTCAAAACTATTCTTCATTCCGATTATCCCGACCACTGTCCCCATCTTCTTGATTGGATCAAACATAATTTACACGATCAACAACAACATCTCTATTCTGCCTTATTCGTTCTCCGCATTCTTTCTATTAAATATGAATTCAAATCAGACGAAGATAGGACTCCTGCCTATCACATTATTCACGAGACATTTCCCCACCTTCTCAATATATTTAACACACTTCTCCAGATTCCTAATCCATCCATTCAAGTTGCTGATCTCATCAAGCTTATCTGTAAAATATTCTGGTCCTCTATATATGTACGTACCTATAACCTACCTTCTCTTATTATTTTCATCATATTCTCCGTCTTCATCTTCATTTTTTTTAAATTTACGCAGTTGGAGATTCCAAAGCTTTTGTTTGATCAAAATGTATTCAGTGCTTGGATGGTGCTGTTTTTAAATGTATTGGAGAGGCCTGTTCCCTTGGAAGGTCAGCCTGTTGATCCTGATCTTCGCAAATCATGGGGTTGGTGGAAACTCAAAAAATGGACAATTCACATTCTAAACAGACTCTACACTCGGTCAGTTTCAATTCTCCATTTCATTTTATTCATTCAAAACAATTCTATTTTCACTCACAATTCCTATGCTTGTTTACAGTTTTGGTGACTTGAAGCTGCGAAACCCTGAAAACAAGGCATTTGCTCAAATGTTTCACAAGCATTATGCGGGGAAGATTTTGGACTGCCACTTAAATCTGTTGAATGTCATTCGAGCAGGTGGTTACTTACCTGACAGAGTTATCAACCTACTTCTTCAATATCTAACCAACAGGTACTTCTATATCATATAAACCTCTTTAGTGGTGTCATTTCATACTTCATAGCATTTAATCCTGTGACACCTATTAGCTGCTCTGTTAAATGGTTCTATTTTCTACCATCAAATTTCAGGACATATTGATTCTTGACTTAATGCTTTCTTTCTGAAATTTTTGAACATGCAGTATTTCAAAGAATAGCATGTATACTGTGCTGCAGCCTCGGCTTGACGTTCTTCTTTTTGAAATAGTTTTCCCTCTCATGTGCTTCAATGATAATGATCAAAAGCTTTGGGACGAAGATCCACATGAGTATGTTAGGAAAGGCTATGGTAAGTCTTATTGGTGTGTAAACATGTAAACTAGATTTCAAATAGTTTGCGTCGTCATGTGCTTATAACTTCGAATTATTTGTTACAGACATTATTGAAGACTTGTATAGTCCTAGGACTGCCTCCGTGGATTTTATGAGTGAGTTGGTTCGAAAGCGCAGGGAAGATAATTTTCACAAGTTTATTCAATTCATTGTTGAAATTTTGAAAAGGTAAATATTTTACTTTCTGTTAAGAATAATAAGATTTATGGATTGGATGCTGTATTTATGTTTTTTCCATTCTAGGTATGATGAAGCACCAGTAGAATACAGGTCTTATAGGCAGAAAGATGGAGCTCTTCTTGCTATTGGGACACTTTGTGACAAATTGAAACAAACTGAGCCCTATAAATCTGAACTTGAGCGTATGTTAGTGCAACACGTCTTTCCCGAGTTTGGCAGTCCTGTTGGCCATCTTAGGGCCAAGGTACGGTTTCCTATACAATTTATATGTGCACATTTTCTGATGCTTGCCAGATTCACTCTCTTTTGTTTTCTCCTTCTATTTATGTATTTTAAATTGGTAACCATTTCACAATATCCACACACAGGGCCGTCTTAACAAATTTGTAGGCCCAGATATAGTCTTTAAAGGGATGTTTTAAATTTTAGGCCCCAAAAAAAATTAAATAAGGCACTATAAAGGAATTTTATGAAAAAAACACACTGGTGAGGCTCGAACTCATGACCATACACCCAAACACAAGCTTCCGCGCCTCTGAAGTATTGCAATCGGATTGAAATTTGTTTGCTTTAGTTGTATATAAGTATTATATTAGTGCCTTAAATACAAAAAAATATATTTTGGGCCCCAAAAATTTTGAGGCCCGGTGACATAGCCCAGCCTCGACGGGCTATGGGCCAGCCCTGTCCACACAAAAGAACATGCTAATTTGATGTCCGTATCCGATAAATGATTTGGGTGCTACAAAAGTTGGGGCATCCAAGTTTTAATAGGTTGGAATCTCTTATGCGCTTATGCGTGTTTCTATGATATTTTATTCGAAACAAAAATCCTCCATGAGTATATAATTATTTTTTTCATCGCACGGGTGTGGTGATGTGAATCACTCTCCTCCTGACTTTCCTTGAAAGAAGGTAAGAGAGGAGACTTTAATTCAAGAATTGAACAAAAAGGGAACTAACGTGATTGTCTTTGCTGTTTTATTTTGTATGGTACAAAGATATTGCAAAACACATTTTTCCTGCTGAAATGTAAATGTACACCAAAAATGTTTTTTCATGTCGCAACCAACCTTATATTCTTCCACATGTTGGGTTATAGCCAAGGGCGAGCCTCATTGAAGTAGAGCTAGTGCTCTTCTCATTCTTTCCTTAATATTGCTTTCACTATAACCAAAGAAAGTATTAGTGTTTTGCTTATACAGTATTGTGATATGACACGACGTAAGTTAATTAGTATTTGAGTAATACATTATGGAACCTTCATTATCTAAAGAATTTTGGCTATCTATTTGTTTTCCTGTGAAAAACAGGCAGCGTGGGTTGCAGGACAATATGCCCACATTAGCTTCTCAGATCAGAACAATTTCCGGAAGGCATTGCAATGTGTTGTCTGTGGAATGCGAGATCCTGAACTACCTGTCCGCATTGATTCTGTTTTTGCTTTGCGTTCTTTTATTGAAGCTTGCAAAGGTGGTCATACTCTCTGTTTATTTTGTGATTTGGATGAATCCTAGTAAAGATTAATCTTGATGAAGCTTATATTTTCATTTTATTGATGCAGATTTAGATGAAATTCGTTCTCTTCTCCCTTCGCTCCTTGATGGTACGTGCATGAAAAAATTGGTGTACATTGTACTTATTTGACTTGGTGAACGAGTTTAACCAACACAAATATTTTCAGAGTTCTTCAAACTTATGAATGAGGTTGAGAATGAAGACCTTGTGTTTACTTTGGAGACTATTGTGGACAAGTTTGGTGAAGAAATGGCACCTTATGCACTTGGGCTATGCCAAAATTTGGTAATTTATATATTTTTAAATTTTTGTATCTGCCAAGAATGATGTCCTTGTTTTGGATGGCTAGTATTTTTCTGTATTGTAAATAGCTCTTTCCTTTCTCAACAAAGGCTGCTGCATTTTGGAGGTGTATGAACACTGCTGATGCTGATGATGACGCTGATGATTCAAGTTCAATGGCTGCAGTTGGTTGCTTGCGTGCTATTAGCACAATACTTGAATCAGTGAGCAGTCTTCCTCAACTTTTTGTTCAAATCGAGCCTACCTTGCTTCCCATAATGCGTAGCATGTTAACAACCGATGGTCAAGGTATTCCCTTATGAATCATGTTTATCTGAATGACTCTGAGGCTTTCTTTTGCTTCTATCAGCTTGTCTTTTCAATGTCATTTTGTGTTTGCTTGCATATACACATTTATTGAGCAAAGGAGATTCTACTTGATTGTATGGATTGGGGTATCACTCATTTAGCATTATTGGTATACTTAAACTTCTACGCATGCTTACAGAGGTTTTTGAAGAAATTTTGGAGATCGTATCATATATGACTTTTTTCTCTCCAACAATATCACTGGACATGTGGAACCTTTGGCCATTGATGATGGAAGCACTATCAGATTGGGCTATTGATTTCTTTTCAAGTATGGCTCTCAACTACATTTGACTGCAATTATCTCAACTTATCTTTGCCTTCCAAAAGTTCAGTACTTTGGACTTCTTTTTTACATGTTAATAACTTAGACAGCTAGAGATGAATCAGTGTCTCCCAGTAAACCTGAAGATAATCTCAGCTATGGACATAATAGAGCATCTTCTGCTTTTTTTTCGTTACACAATCCATGTTTGTTACTTAATAAAAGGGTGCTATTTTTCTGGATCTGATCCATACTATTCAAAAACATGGAATATCTTTCAAATATTTTCCTGGTTGCTCTGACATTTAAGTTAATATAAGTCTTTATATATTTTTACATTTTCTACATGCTTACTCGATTAGTTTATTCTGCAGATATTATGGTTCCTTTGGACAATTATATTTCAAGGGGAACTTCCCATTTCATTACATGCAAAGAACCTGACTATCAACAGTGTTTGTGGAATATGATTTCCTCTGTAAGTGATCTTTGAATTTTTATCATTAATTTGGCCACCACCTGTCAGGACTGGAGTGGGATCTGGTGATTGTAAATGATTGTTAACTTCTTATTTTTCTAGCTTCTTCTTGTTGTCTCTGATTTGTATTTCACATTGCAGATTATGTCAGATAAAAATATGGTGGACAGTGATATTGAGTCAGCTCCAAAGCTGATTGAAATTGTCTTCCTAAACTGCAGAGGACTGGTGGACCACTGGGTTGAGCCGTATCTCAGAATTACAATTGAACGTTTGCATAGAACTGGAAAATCATATTTGAAATGTCTTCTCATTCAAGTGGTAAGGTTTTAATTGAATCTTTATTGACATGCACAAATAAGATAGTTGGCTCCTGTTGCTTTCTTTCTTAAAAGCTGGATTTTCGATGGTCCATTATACCATGTTTTCTTTTCAACCCATGTTTCTATTTTGAACATTGGCTTACGAGCTACAACAAGTGCGTGGGTTATAAGAAGTTGGATGCATCTAATTTTGTATGGCAGTTTTTTATGCAATTGCTTTGTTCGTGATTCTTTTACTGAGTTCAAATTTATAGAGCTAAATCAACTGCTACGAACCACTTGTCGGATGAAATTTATTCATTCATTTTTCAATGAGTGCAGTTTATCTATCTTGTAAGTTAGCCAAATTTCTTAAAGATAATAGGGAAAGACACCAAATGCAACTTCATAAGAGGATATTTTTTTTTGCAGATTTCAGATGGTCTGTACTACAACGCCACATTGACTCTTAGCATATTGCAAAAGTTAGGTGTTGCATCAGAAATCTTCAATCTTTGGTTCCAAATGCTGCAAGAAGTCAAGAAGAGTGGTGTGCGTGCTAATTTTAAAAGGTCTGTGTCATTGCTTTTCACTGTCCCTCCTCCTTGCCTCAATTACTTACTCCATACTTTGTTTTCACATTGCAATATAAGTTATTGTGTGTAGCTATGTGAAGGTCTATTCACTGCAGACTTGTGACTATTTTTATGCAGGGAACATGGCAAGAAAGTTTGCTGCCTTGGTTTAACATCCTTGCTGGCACTTCCAGCTGATCAATTGCCAGGAGAGGCTTTAGGCCGGGTATTCCGTGCCACTCTTGATCTCCTGGTTGCATACAAGGATCAAGTTGCAGGTCTTTATGTCACACATTCTCCGTAACTCTTTTATTTATTTTTTTGACTCAACTTTCTCCGTAACTCTGACATGTTGAGGACTTGTAAATTACAATATGATTGTCAGGAATAAACTGACGTCCATTTCTGTTTCTCTACTATTGTACTTTGCAGAAGCTGCAGAAGAGGAGGAAGAAGAAGAAGAAAATGACGACGATGATATGGACGGCTTTCAAACTGATGATGAAGATGAGGATGACAGTGGTTCTGACAGGGAAATGGGAACTGATGCTGAAGATGGGGATGAAGCTGACAGTAGTAAACTTAGAAAATTGACTGATCAGGTCCGTGGATATAATTCCGAAGACTCAAAGAACATTTGCATCCATATATTAACTAAATATAGGATATTGAGTTTACCAATCTGCATGCAAAAAAGAAAGGTTGCAATCTGCACACAGGAAATCTAGTAATTCACGATTTGCCAAGTTCGTTATTGCTAGAAATGTTTGATATCATTCACGTGTTTAGAATATTTGTAAAATATTTTAATCTGTATAGGTTTTATAATTATTGAACTGTAGAATATTTGAATTTTTAGTGTCATGATTTTTATTGTTTTGCTTTTTGTTTTTTTTAACATGTTTTATTGTTTACTACTAGGAAAAAGTACCAAATTTGACATAGTATAAAGTCTAGACAATTTGTGTGCCTTATAAGTCAATTTTGTGAGGCTGAGTTAGTCCCTGAAACTTCGTAATTAGTTTCAAAGTAGTAAGTTTCTGCAATTCAGTATAAATAAGAAACAAAGTATGATTCCATTAGGTATGATTCCCTGCCCAGAACTTTTGCTTAAAGTAATGTTATTGTTCACCCAGTAGAAGTATTCGCCATTTTGTTAACTTAATGATTTTTGACAAGAAACCATAGTTTATTTGAATTATAGACTAGTTCAGTGAACCTACATAAGGTGCTTAACCGATCCAGTAAACCATCCAGCTACACAATTTTGTGGTTGAACCAGCTGGTTTGTGTCGAACTATTGTCTTCTTGATTCATATTTGTATCTTTTAGGAATGAAACTTGTTTTCCTTTCTCTTGGAAGATAAAATATAAATCGTGCACTTACAGTTTAGATTTGTGATTTAACATTTGTTTTCATTTTATCATTTTATATCTTAAATTCTTATTGTCATTTGCAATGTCATCCAATAGGAAACGTCATTCAGGCCCGATGATGAGGACTCAGATGACGACTTCAGTGATGACGAAGAGTTACAGTCCCCAATTGATGAAGTGGATCCTTTTATTTTTTTTGTGGATACAATGAAAGGTATGCCGATACTGCACCTTAAAGCATGTTAAAGGTTTCATTTACCAATACCTATCATTATTGCTATAATTGTCTGACATATCATCTGTATTCGAAATAAATTTGTCTGTAGTGATGCAATCATCAGATCCAATGAGGTTTCAAAACCTGACCCAAACACTTGAGTTCTCTTATCAAGCTCTTGCAAATGGTGTTGCCCAGCATGCTGAGACGAGAAGAGGTGAGATTGAGAAGGAAAAGTCGGAGAAGTCAGCAGCTACCACAGATTCTTAA
Protein sequence

>MS.gene90905.t1

MDEVVVILEGTLSPNPNERKAAEQRLDEIQYAPNHLPTLLQIIVQSDDSVSDISLRQVAAIHFKNFIAKNWSSTAHSISFADKDFVRNHILLFLPQLPSLLRVQLGECLKTILHSDYPDHCPHLLDWIKHNLHDQQQHLYSALFVLRILSIKYEFKSDEDRTPAYHIIHETFPHLLNIFNTLLQIPNPSIQVADLIKLICKIFWSSIYLEIPKLLFDQNVFSAWMVLFLNVLERPVPLEGQPVDPDLRKSWGWWKLKKWTIHILNRLYTRFGDLKLRNPENKAFAQMFHKHYAGKILDCHLNLLNVIRAGGYLPDRVINLLLQYLTNSISKNSMYTVLQPRLDVLLFEIVFPLMCFNDNDQKLWDEDPHEYVRKGYDIIEDLYSPRTASVDFMSELVRKRREDNFHKFIQFIVEILKRYDEAPVEYRSYRQKDGALLAIGTLCDKLKQTEPYKSELERMLVQHVFPEFGSPVGHLRAKAAWVAGQYAHISFSDQNNFRKALQCVVCGMRDPELPVRIDSVFALRSFIEACKDLDEIRSLLPSLLDEFFKLMNEVENEDLVFTLETIVDKFGEEMAPYALGLCQNLAAAFWRCMNTADADDDADDSSSMAAVGCLRAISTILESVSSLPQLFVQIEPTLLPIMRSMLTTDGQEVFEEILEIVSYMTFFSPTISLDMWNLWPLMMEALSDWAIDFFSNIMVPLDNYISRGTSHFITCKEPDYQQCLWNMISSIMSDKNMVDSDIESAPKLIEIVFLNCRGLVDHWVEPYLRITIERLHRTGKSYLKCLLIQVISDGLYYNATLTLSILQKLGVASEIFNLWFQMLQEVKKSGVRANFKREHGKKVCCLGLTSLLALPADQLPGEALGRVFRATLDLLVAYKDQVAEAAEEEEEEEENDDDDMDGFQTDDEDEDDSGSDREMGTDAEDGDEADSSKLRKLTDQETSFRPDDEDSDDDFSDDEELQSPIDEVDPFIFFVDTMKVMQSSDPMRFQNLTQTLEFSYQALANGVAQHAETRRGEIEKEKSEKSAATTDS