Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91106.t1 | XP_003589727.2 | 97.9 | 615 | 11 | 1 | 1 | 613 | 1 | 615 | 0.00E+00 | 1173.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91106.t1 | Q0WQE7 | 73.5 | 614 | 154 | 4 | 1 | 612 | 1 | 607 | 1.4e-254 | 880.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91106.t1 | G7IBE5 | 97.9 | 615 | 11 | 1 | 1 | 613 | 1 | 615 | 0.0e+00 | 1173.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059154 | MS.gene91106 | 0.819554 | 1.09E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91106.t1 | MTR_1g038570 | 98.374 | 615 | 8 | 1 | 1 | 613 | 1 | 615 | 0.0 | 1246 |
| MS.gene91106.t1 | MTR_1g038570 | 96.260 | 615 | 8 | 2 | 1 | 613 | 1 | 602 | 0.0 | 1209 |
| MS.gene91106.t1 | MTR_1g038570 | 98.800 | 500 | 6 | 0 | 114 | 613 | 1 | 500 | 0.0 | 1016 |
| MS.gene91106.t1 | MTR_1g038570 | 98.600 | 500 | 6 | 1 | 114 | 613 | 1 | 499 | 0.0 | 1009 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91106.t1 | AT5G48520 | 74.267 | 614 | 149 | 4 | 1 | 612 | 1 | 607 | 0.0 | 943 |
| MS.gene91106.t1 | AT5G48520 | 73.941 | 614 | 152 | 3 | 1 | 612 | 1 | 608 | 0.0 | 941 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGGTTGATGACAGCCTTTC+TGG | 0.126372 | 1.3:-21863298 | MS.gene91106:CDS |
| TAAGAATCAAGGTAAATTAT+TGG | 0.253282 | 1.3:-21864870 | MS.gene91106:CDS |
| GCTAGTTCCCAACACAATTC+TGG | 0.263214 | 1.3:+21856753 | None:intergenic |
| CCTTTCTGGAAGAAATTTAC+AGG | 0.277249 | 1.3:-21863284 | MS.gene91106:intron |
| TTCAGAGTGGGAGAGAATAT+TGG | 0.285456 | 1.3:+21865014 | None:intergenic |
| TGCAGAAGATGGTATATATT+TGG | 0.293364 | 1.3:-21862562 | MS.gene91106:intron |
| ATTATTGGAAGGTGATGATT+TGG | 0.296294 | 1.3:-21864855 | MS.gene91106:CDS |
| ATCTTGACCACCATTCTCTT+AGG | 0.314638 | 1.3:-21858247 | MS.gene91106:intron |
| TGCACATTACTCGAAGAATT+AGG | 0.325419 | 1.3:-21865153 | MS.gene91106:CDS |
| TGCTTCCACAGCAGAGGAAT+TGG | 0.344725 | 1.3:-21862724 | MS.gene91106:CDS |
| GAATTGGCTCATTACCATTC+AGG | 0.345726 | 1.3:-21862708 | MS.gene91106:CDS |
| ATACACCTATTTCGTTCTTC+AGG | 0.347755 | 1.3:+21854250 | None:intergenic |
| GTTGTTGCATACTTTGAATA+AGG | 0.347767 | 1.3:+21853812 | None:intergenic |
| TTCCGATACTACGGATAATC+AGG | 0.350115 | 1.3:-21864804 | MS.gene91106:CDS |
| GAAATCAGGTTCAGATTCAT+TGG | 0.360896 | 1.3:-21865118 | MS.gene91106:CDS |
| CTCTTCCTGAAGAACGAAAT+AGG | 0.365348 | 1.3:-21854255 | MS.gene91106:CDS |
| ACTTGGATCATCAACCGTTC+TGG | 0.370039 | 1.3:+21852427 | None:intergenic |
| GGTTACGAAGGAACGAAATC+AGG | 0.376148 | 1.3:-21865132 | MS.gene91106:CDS |
| TCTTGACATGCTCTAGAGTC+AGG | 0.382431 | 1.3:+21852817 | None:intergenic |
| GTCTCAAGTAATTATGTTAG+AGG | 0.382741 | 1.3:-21860322 | MS.gene91106:intron |
| AATGAAGAGCTGATATTTGC+TGG | 0.384263 | 1.3:+21854314 | None:intergenic |
| TCATGAACCACTTAATTTGA+AGG | 0.407052 | 1.3:-21864765 | MS.gene91106:CDS |
| TTGATATCCTTCAAATTAAG+TGG | 0.407958 | 1.3:+21864758 | None:intergenic |
| ATATGTTGACAGTGCGGCTT+GGG | 0.411185 | 1.3:+21854357 | None:intergenic |
| GAAGTTCATATTCTTGTTGA+TGG | 0.413926 | 1.3:+21852482 | None:intergenic |
| ATACTCGACCGATTCTTCAT+TGG | 0.415018 | 1.3:-21865059 | MS.gene91106:CDS |
| AGGGGAAATCTAAATGCTCT+TGG | 0.419902 | 1.3:-21860360 | MS.gene91106:CDS |
| GTTCGACATGCTTTCTAGCC+AGG | 0.420078 | 1.3:-21863389 | MS.gene91106:CDS |
| ATCTCATCAATCTGCTTGCC+AGG | 0.422894 | 1.3:-21855282 | MS.gene91106:CDS |
| TTTCGCGTTTGATAGTATTT+CGG | 0.433168 | 1.3:-21864831 | MS.gene91106:CDS |
| TCTAGGTGATTATGATTTGA+AGG | 0.437034 | 1.3:-21856613 | MS.gene91106:intron |
| GGCGCGACTTCTGTTGTTAA+CGG | 0.439961 | 1.3:-21863331 | MS.gene91106:CDS |
| TCCTCAGCAACAAGTCGAAA+TGG | 0.440782 | 1.3:+21860382 | None:intergenic |
| GACTATTCCAGAATTGTGTT+GGG | 0.441135 | 1.3:-21856760 | MS.gene91106:CDS |
| TGGTTTGCAGGGTCGTTGTC+TGG | 0.444294 | 1.3:-21854586 | MS.gene91106:intron |
| GCCGGTTAATGTAGTACTCT+TGG | 0.446595 | 1.3:+21856583 | None:intergenic |
| AGACTATTCCAGAATTGTGT+TGG | 0.446623 | 1.3:-21856761 | MS.gene91106:CDS |
| TTGCAGTTAGTTCACTAACT+TGG | 0.449797 | 1.3:+21852410 | None:intergenic |
| CGAGTATCTTCGTACTGAAA+TGG | 0.455647 | 1.3:+21865075 | None:intergenic |
| GCCAAGAGTACTACATTAAC+CGG | 0.457163 | 1.3:-21856584 | MS.gene91106:CDS |
| GATCAGATGAATGCAGTTCT+CGG | 0.464437 | 1.3:-21862753 | MS.gene91106:intron |
| CATTAAAATCGCCTGCTGCT+TGG | 0.465881 | 1.3:+21858310 | None:intergenic |
| GCAACTCAGACACACGTTGA+TGG | 0.470517 | 1.3:+21859744 | None:intergenic |
| AGATAGAGCAAAGGCGAAGA+AGG | 0.474096 | 1.3:+21852154 | None:intergenic |
| CGGAATAAGTGAAAGACAAT+GGG | 0.482886 | 1.3:-21858354 | MS.gene91106:CDS |
| CCTGTAAATTTCTTCCAGAA+AGG | 0.483290 | 1.3:+21863284 | None:intergenic |
| CACAGCCAATACTGACCCCT+CGG | 0.490718 | 1.3:-21853765 | MS.gene91106:CDS |
| ACTTGTTAACTGATATCAAA+CGG | 0.491381 | 1.3:+21852125 | None:intergenic |
| AAATATTACCATCTCCTGAA+TGG | 0.492014 | 1.3:+21862694 | None:intergenic |
| AAATGGCCATTCGAAGCTAT+CGG | 0.495886 | 1.3:+21865092 | None:intergenic |
| TATCTTTAAGAGCTGATGCC+TGG | 0.496300 | 1.3:+21855264 | None:intergenic |
| CAAGGAAGGCGAGCGCGTGT+TGG | 0.496987 | 1.3:-21863352 | MS.gene91106:CDS |
| TATGAGCAGTTTAAGAATCA+AGG | 0.497862 | 1.3:-21864881 | MS.gene91106:intron |
| CTTCAGGAAGAGAATTATCA+AGG | 0.498387 | 1.3:+21854266 | None:intergenic |
| CATACCCGAGGGGTCAGTAT+TGG | 0.498952 | 1.3:+21853760 | None:intergenic |
| GGGGAAATCTAAATGCTCTT+GGG | 0.501644 | 1.3:-21860359 | MS.gene91106:CDS |
| AGCATACTTGTCTGCAACAG+AGG | 0.502173 | 1.3:-21855170 | MS.gene91106:CDS |
| GATATTTGCTGGACGATGCC+TGG | 0.505574 | 1.3:+21854325 | None:intergenic |
| ATTTCGACTTGTTGCTGAGG+AGG | 0.509755 | 1.3:-21860380 | MS.gene91106:CDS |
| GCTCATTACCATTCAGGAGA+TGG | 0.510414 | 1.3:-21862702 | MS.gene91106:intron |
| TTTCGACTTGTTGCTGAGGA+GGG | 0.511232 | 1.3:-21860379 | MS.gene91106:CDS |
| GAGCAGATCCAATGAAGAAT+CGG | 0.515097 | 1.3:+21865051 | None:intergenic |
| AGTTGAGAATGCCAAGCAGC+AGG | 0.516645 | 1.3:-21858321 | MS.gene91106:CDS |
| GCATCTGATGGTCAAGAACA+AGG | 0.520173 | 1.3:-21854549 | MS.gene91106:CDS |
| AATGGCCATTCGAAGCTATC+GGG | 0.520913 | 1.3:+21865093 | None:intergenic |
| AGAAAGCATTCTGAATTGAA+AGG | 0.522631 | 1.3:-21857864 | MS.gene91106:CDS |
| TTCGACTTGTTGCTGAGGAG+GGG | 0.524104 | 1.3:-21860378 | MS.gene91106:CDS |
| CATATGTTGACAGTGCGGCT+TGG | 0.530335 | 1.3:+21854356 | None:intergenic |
| CTTGACCATCAGATGCAGCT+TGG | 0.531465 | 1.3:+21854556 | None:intergenic |
| AATATTGGAAGGACGAAGAG+TGG | 0.534137 | 1.3:+21865029 | None:intergenic |
| TTGGAAAAGAAGCACATGCT+TGG | 0.537163 | 1.3:-21855232 | MS.gene91106:CDS |
| CTTGCGTCAATGTCGAAGCC+TGG | 0.540301 | 1.3:+21863371 | None:intergenic |
| AGGCAAATTACCTAAGAGAA+TGG | 0.543877 | 1.3:+21858237 | None:intergenic |
| CTTATCCAAGCTGCATCTGA+TGG | 0.552561 | 1.3:-21854561 | MS.gene91106:CDS |
| GATGAAAGAGCTCGATGAGA+TGG | 0.553044 | 1.3:-21852877 | MS.gene91106:CDS |
| AATCAAGGTAAATTATTGGA+AGG | 0.558038 | 1.3:-21864866 | MS.gene91106:CDS |
| ATAAACATACCGCTTTCTGC+CGG | 0.560499 | 1.3:+21856565 | None:intergenic |
| CTTCGACATTGACGCAAGGA+AGG | 0.562210 | 1.3:-21863366 | MS.gene91106:CDS |
| CTGATTTGCAAAGACAGCTA+AGG | 0.563179 | 1.3:-21863426 | MS.gene91106:CDS |
| TCGGAATAAGTGAAAGACAA+TGG | 0.566089 | 1.3:-21858355 | MS.gene91106:CDS |
| CACAAAGCTCTCTCTTGCCG+TGG | 0.568172 | 1.3:-21852844 | MS.gene91106:CDS |
| AACAAGAATATGAACTTCAA+CGG | 0.569950 | 1.3:-21852477 | MS.gene91106:CDS |
| TTCCTGATTATCCGTAGTAT+CGG | 0.572445 | 1.3:+21864802 | None:intergenic |
| TCCATTTCGACTTGTTGCTG+AGG | 0.575197 | 1.3:-21860383 | MS.gene91106:CDS |
| CTCTAGAGTCAGGTCTTCCA+CGG | 0.579829 | 1.3:+21852827 | None:intergenic |
| TGGCAAGCAGATTGATGAGA+TGG | 0.586846 | 1.3:+21855284 | None:intergenic |
| CATCTGATGGTCAAGAACAA+GGG | 0.588360 | 1.3:-21854548 | MS.gene91106:CDS |
| ATGAGCCAATTCCTCTGCTG+TGG | 0.589754 | 1.3:+21862719 | None:intergenic |
| TTCAAATTAAGTGGTTCATG+AGG | 0.589983 | 1.3:+21864767 | None:intergenic |
| TCATCGAGCTCTTTCATCAA+CGG | 0.598648 | 1.3:+21852882 | None:intergenic |
| CTCGAAGAATTAGGTTACGA+AGG | 0.603206 | 1.3:-21865144 | MS.gene91106:CDS |
| ATCTGATGGTCAAGAACAAG+GGG | 0.610072 | 1.3:-21854547 | MS.gene91106:CDS |
| AGGAAGAGAATTATCAAGGT+CGG | 0.610949 | 1.3:+21854270 | None:intergenic |
| AAGAATTGCTTCCACAGCAG+AGG | 0.613594 | 1.3:-21862730 | MS.gene91106:CDS |
| ACAGCCAATACTGACCCCTC+GGG | 0.617634 | 1.3:-21853764 | MS.gene91106:intron |
| AAGTTAGTGAACTAACTGCA+AGG | 0.618460 | 1.3:-21852408 | MS.gene91106:CDS |
| GTCTGCAACAGAGGGTCGAG+TGG | 0.618841 | 1.3:-21855161 | MS.gene91106:intron |
| CTTAAAGATAGCCTGCCAGT+TGG | 0.625364 | 1.3:-21855251 | MS.gene91106:CDS |
| GAACTGAGAGAGTTCAGAGT+GGG | 0.637197 | 1.3:+21865002 | None:intergenic |
| GCATACTTGTCTGCAACAGA+GGG | 0.637772 | 1.3:-21855169 | MS.gene91106:CDS |
| AACGTGTGTCTGAGTTGCAG+CGG | 0.638590 | 1.3:-21859739 | MS.gene91106:CDS |
| AGTTAGTGAACTAACTGCAA+GGG | 0.642599 | 1.3:-21852407 | MS.gene91106:intron |
| GAGTGGGAGAGAATATTGGA+AGG | 0.648895 | 1.3:+21865018 | None:intergenic |
| TCAACATATGTATCAGCACC+AGG | 0.649058 | 1.3:-21854343 | MS.gene91106:CDS |
| CTACATTAACCGGCAGAAAG+CGG | 0.650197 | 1.3:-21856574 | MS.gene91106:intron |
| CGGTGAGAAAGATAGAGCAA+AGG | 0.652138 | 1.3:+21852145 | None:intergenic |
| CCCAGAGAAACGAAAATGAG+CGG | 0.652627 | 1.3:-21865186 | None:intergenic |
| GTCTAAAAGAGCTAAATCAG+TGG | 0.655154 | 1.3:-21862497 | MS.gene91106:CDS |
| TGGATCCCGATAGCTTCGAA+TGG | 0.656716 | 1.3:-21865098 | MS.gene91106:CDS |
| CAAATTACCTAAGAGAATGG+TGG | 0.658037 | 1.3:+21858240 | None:intergenic |
| GGCTGTCATCAACCGCTGTG+AGG | 0.667238 | 1.3:+21863305 | None:intergenic |
| CAGGCTTCGACATTGACGCA+AGG | 0.671499 | 1.3:-21863370 | MS.gene91106:CDS |
| CGAACTGAGAGAGTTCAGAG+TGG | 0.691382 | 1.3:+21865001 | None:intergenic |
| ATATGAGATTCATACCCGAG+GGG | 0.770208 | 1.3:+21853750 | None:intergenic |
| TGATACATATGTTGACAGTG+CGG | 0.790586 | 1.3:+21854351 | None:intergenic |
| TCTGATGGTCAAGAACAAGG+GGG | 0.803663 | 1.3:-21854546 | MS.gene91106:CDS |
| TGTTAACGGACACCTCACAG+CGG | 0.808466 | 1.3:-21863317 | MS.gene91106:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 21852130 | 21865193 | 21852130 | ID=MS.gene91106 |
| chr1.3 | mRNA | 21852130 | 21865193 | 21852130 | ID=MS.gene91106.t1;Parent=MS.gene91106 |
| chr1.3 | exon | 21865002 | 21865193 | 21865002 | ID=MS.gene91106.t1.exon1;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21865002 | 21865193 | 21865002 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21864758 | 21864894 | 21864758 | ID=MS.gene91106.t1.exon2;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21864758 | 21864894 | 21864758 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21863285 | 21863480 | 21863285 | ID=MS.gene91106.t1.exon3;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21863285 | 21863480 | 21863285 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21862703 | 21862769 | 21862703 | ID=MS.gene91106.t1.exon4;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21862703 | 21862769 | 21862703 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21862476 | 21862579 | 21862476 | ID=MS.gene91106.t1.exon5;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21862476 | 21862579 | 21862476 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21860323 | 21860407 | 21860323 | ID=MS.gene91106.t1.exon6;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21860323 | 21860407 | 21860323 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21859728 | 21859782 | 21859728 | ID=MS.gene91106.t1.exon7;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21859728 | 21859782 | 21859728 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21858248 | 21858379 | 21858248 | ID=MS.gene91106.t1.exon8;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21858248 | 21858379 | 21858248 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21857818 | 21857887 | 21857818 | ID=MS.gene91106.t1.exon9;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21857818 | 21857887 | 21857818 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21856724 | 21856781 | 21856724 | ID=MS.gene91106.t1.exon10;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21856724 | 21856781 | 21856724 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21856575 | 21856630 | 21856575 | ID=MS.gene91106.t1.exon11;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21856575 | 21856630 | 21856575 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21855162 | 21855314 | 21855162 | ID=MS.gene91106.t1.exon12;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21855162 | 21855314 | 21855162 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21854487 | 21854598 | 21854487 | ID=MS.gene91106.t1.exon13;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21854487 | 21854598 | 21854487 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21854241 | 21854382 | 21854241 | ID=MS.gene91106.t1.exon14;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21854241 | 21854382 | 21854241 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21853765 | 21853840 | 21853765 | ID=MS.gene91106.t1.exon15;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21853765 | 21853840 | 21853765 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21852809 | 21852904 | 21852809 | ID=MS.gene91106.t1.exon16;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21852809 | 21852904 | 21852809 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21852408 | 21852515 | 21852408 | ID=MS.gene91106.t1.exon17;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21852408 | 21852515 | 21852408 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| chr1.3 | exon | 21852130 | 21852195 | 21852130 | ID=MS.gene91106.t1.exon18;Parent=MS.gene91106.t1 |
| chr1.3 | CDS | 21852130 | 21852195 | 21852130 | ID=cds.MS.gene91106.t1;Parent=MS.gene91106.t1 |
| Gene Sequence |
| Protein sequence |
