AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91209

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91209.t1 MTR_2g437380 65.266 357 28 6 1 263 1 355 1.43e-152 430
MS.gene91209.t1 MTR_2g437380 60.317 252 25 5 85 263 12 261 2.69e-92 273
MS.gene91209.t1 MTR_7g063730 31.778 343 137 8 14 263 26 364 3.56e-42 148
MS.gene91209.t1 MTR_7g062580 30.029 343 143 10 14 263 17 355 2.09e-40 143
MS.gene91209.t1 MTR_2g072910 29.480 346 142 11 14 263 14 353 1.97e-38 138
MS.gene91209.t1 MTR_7g062550 28.571 343 148 9 14 263 22 360 3.42e-36 132
MS.gene91209.t1 MTR_7g062570 29.738 343 144 10 14 263 17 355 5.68e-36 131
MS.gene91209.t1 MTR_7g062600 28.818 347 143 11 14 263 13 352 1.44e-31 119
MS.gene91209.t1 MTR_2g072890 26.590 346 153 10 14 263 14 354 2.10e-31 119
MS.gene91209.t1 MTR_5g048550 28.070 342 150 9 14 263 10 347 6.20e-30 115
MS.gene91209.t1 MTR_3g026150 27.761 335 142 10 14 253 21 350 2.98e-26 105
MS.gene91209.t1 MTR_5g082750 25.460 326 148 9 14 244 11 336 1.00e-25 103
MS.gene91209.t1 MTR_3g117420 28.527 319 122 9 43 263 43 353 3.43e-25 102
MS.gene91209.t1 MTR_8g074070 28.704 324 144 11 17 260 15 331 1.29e-24 100
MS.gene91209.t1 MTR_7g068650 26.126 333 142 10 14 244 12 342 3.80e-24 99.8
MS.gene91209.t1 MTR_4g015790 23.714 350 156 9 17 256 14 362 8.17e-22 93.6
MS.gene91209.t1 MTR_8g074070 27.114 343 144 11 17 260 15 350 9.25e-22 93.2
MS.gene91209.t1 MTR_3g070860 22.769 325 152 9 17 244 15 337 2.17e-21 92.0
MS.gene91209.t1 MTR_5g048740 43.519 108 53 1 164 263 239 346 5.36e-21 90.9
MS.gene91209.t1 MTR_5g048570 39.683 126 62 3 152 263 220 345 1.53e-20 89.7
MS.gene91209.t1 MTR_5g048570 30.469 128 63 3 14 120 10 132 2.87e-11 63.2
MS.gene91209.t1 MTR_0009s0400 37.963 108 59 1 164 263 36 143 1.56e-20 85.5
MS.gene91209.t1 MTR_0009s0050 38.889 108 58 1 164 263 60 167 2.09e-20 85.9
MS.gene91209.t1 MTR_8g024120 39.815 108 57 1 164 263 235 342 3.21e-20 89.0
MS.gene91209.t1 MTR_8g074050 26.752 314 128 9 43 260 43 350 4.21e-20 88.6
MS.gene91209.t1 MTR_5g032870 35.366 164 78 5 120 259 179 338 4.25e-20 88.6
MS.gene91209.t1 MTR_2g072910 27.341 267 100 10 14 192 14 274 7.00e-20 87.0
MS.gene91209.t1 MTR_0009s0050 38.889 108 58 1 164 263 142 249 1.22e-19 85.9
MS.gene91209.t1 MTR_5g055680 38.889 108 58 1 164 263 240 347 1.26e-19 87.4
MS.gene91209.t1 MTR_0009s0050 38.889 108 58 1 164 263 142 249 1.30e-19 85.9
MS.gene91209.t1 MTR_5g048850 38.889 108 58 1 164 263 240 347 2.90e-19 86.3
MS.gene91209.t1 MTR_0009s0050 38.889 108 58 1 164 263 240 347 3.39e-19 85.9
MS.gene91209.t1 MTR_0009s0050 38.889 108 58 1 164 263 240 347 3.73e-19 85.9
MS.gene91209.t1 MTR_5g032880 34.839 155 71 4 120 248 171 321 4.07e-19 87.0
MS.gene91209.t1 MTR_5g032880 32.484 157 80 4 120 252 517 671 3.71e-16 78.2
MS.gene91209.t1 MTR_7g062560 39.604 101 53 1 164 256 245 345 5.80e-19 85.5
MS.gene91209.t1 MTR_8g064200 40.741 108 56 1 164 263 246 353 5.94e-19 85.5
MS.gene91209.t1 MTR_8g064200 40.741 108 56 1 164 263 251 358 6.44e-19 85.5
MS.gene91209.t1 MTR_5g055900 38.889 108 58 1 164 263 240 347 7.85e-19 85.1
MS.gene91209.t1 MTR_0009s0480 37.037 108 60 1 164 263 239 346 1.18e-18 84.3
MS.gene91209.t1 MTR_5g055690 36.111 108 61 1 164 263 241 348 1.26e-18 84.3
MS.gene91209.t1 MTR_0328s0030 40.741 108 56 1 164 263 238 345 1.41e-18 84.3
MS.gene91209.t1 MTR_5g055820 38.095 105 57 1 164 260 241 345 1.72e-18 84.0
MS.gene91209.t1 MTR_0056s0160 39.815 108 57 1 164 263 125 232 2.83e-18 81.6
MS.gene91209.t1 MTR_5g048470 38.889 108 58 1 164 263 247 354 5.78e-18 82.8
MS.gene91209.t1 MTR_5g048470 30.469 128 67 2 14 120 15 141 7.96e-12 64.7
MS.gene91209.t1 MTR_7g045650 37.778 135 57 4 14 125 22 152 6.99e-18 82.0
MS.gene91209.t1 MTR_6g015950 44.086 93 44 1 164 248 231 323 9.18e-18 81.6
MS.gene91209.t1 MTR_3g026150 28.512 242 97 7 85 253 9 247 1.49e-17 80.1
MS.gene91209.t1 MTR_3g092890 36.697 109 61 1 163 263 228 336 1.28e-16 78.6
MS.gene91209.t1 MTR_4g087140 33.333 153 76 4 120 247 179 330 1.38e-16 78.6
MS.gene91209.t1 MTR_5g055800 37.037 108 59 2 164 263 240 346 1.49e-16 78.6
MS.gene91209.t1 MTR_8g009080 37.838 111 58 2 164 263 286 396 1.50e-16 79.0
MS.gene91209.t1 MTR_3g063180 35.780 109 58 2 164 260 240 348 2.05e-16 78.2
MS.gene91209.t1 MTR_8g009090 37.037 108 55 3 164 259 253 359 4.82e-16 77.0
MS.gene91209.t1 MTR_1g034140 40.659 91 44 2 164 244 228 318 5.04e-16 77.0
MS.gene91209.t1 MTR_8g062600 35.088 114 60 2 164 263 259 372 8.21e-16 76.6
MS.gene91209.t1 MTR_4g087110 29.878 164 88 4 120 258 179 340 1.67e-15 75.5
MS.gene91209.t1 MTR_7g081710 36.792 106 58 2 164 260 242 347 2.58e-15 75.1
MS.gene91209.t1 MTR_8g469400 34.545 110 58 2 164 259 246 355 2.69e-15 75.1
MS.gene91209.t1 MTR_4g093840 25.246 305 136 10 47 260 59 362 3.24e-15 74.7
MS.gene91209.t1 MTR_2g069300 36.036 111 60 2 164 263 247 357 4.43e-15 74.3
MS.gene91209.t1 MTR_3g072810 39.326 89 46 1 164 244 233 321 1.48e-14 72.8
MS.gene91209.t1 MTR_1g093120 38.318 107 55 2 164 259 259 365 1.50e-14 72.8
MS.gene91209.t1 MTR_8g009110 34.579 107 59 2 164 259 254 360 1.61e-14 72.8
MS.gene91209.t1 MTR_1g070085 38.298 94 50 1 163 248 152 245 1.72e-14 71.6
MS.gene91209.t1 MTR_2g025120 35.294 102 57 2 164 256 194 295 1.84e-14 72.4
MS.gene91209.t1 MTR_8g008930 36.111 108 56 3 164 259 254 360 1.86e-14 72.8
MS.gene91209.t1 MTR_8g009180 32.710 107 61 2 164 259 183 289 2.15e-14 71.6
MS.gene91209.t1 MTR_1g070085 38.298 94 50 1 163 248 230 323 2.32e-14 72.0
MS.gene91209.t1 MTR_1g070085 38.298 94 50 1 163 248 230 323 2.32e-14 72.0
MS.gene91209.t1 MTR_1g033810 34.653 101 58 1 164 256 242 342 2.37e-14 72.4
MS.gene91209.t1 MTR_1g033810 35.043 117 49 4 27 119 22 135 4.81e-11 62.4
MS.gene91209.t1 MTR_3g117485 35.461 141 62 5 123 237 120 257 3.55e-14 70.9
MS.gene91209.t1 MTR_2g069190 33.645 107 60 2 164 259 260 366 5.71e-14 71.2
MS.gene91209.t1 MTR_3g117485 35.461 141 62 5 123 237 192 329 6.79e-14 70.9
MS.gene91209.t1 MTR_4g087130 36.634 101 54 2 164 254 235 335 6.86e-14 70.9
MS.gene91209.t1 MTR_4g087090 36.000 100 54 2 164 253 235 334 7.02e-14 70.9
MS.gene91209.t1 MTR_1g070110 38.710 93 49 1 164 248 126 218 7.74e-14 69.3
MS.gene91209.t1 MTR_1g070100 39.326 89 46 1 163 243 148 236 1.15e-13 69.3
MS.gene91209.t1 MTR_3g083370 37.624 101 54 2 164 256 194 293 1.20e-13 69.7
MS.gene91209.t1 MTR_4g074130 40.449 89 39 2 164 244 232 314 1.26e-13 70.1
MS.gene91209.t1 MTR_1g034060 40.659 91 44 2 164 244 238 328 1.29e-13 70.1
MS.gene91209.t1 MTR_1g034120 36.634 101 54 2 154 244 228 328 1.45e-13 70.1
MS.gene91209.t1 MTR_2g069090 34.545 110 60 3 164 261 261 370 1.77e-13 69.7
MS.gene91209.t1 MTR_8g009160 31.776 107 62 2 164 259 253 359 1.94e-13 69.7
MS.gene91209.t1 MTR_3g091360 35.185 108 61 2 164 263 247 353 2.15e-13 69.3
MS.gene91209.t1 MTR_2g069210 35.185 108 59 3 164 260 256 363 2.26e-13 69.3
MS.gene91209.t1 MTR_3g026150 31.783 129 64 3 14 120 21 147 2.64e-13 68.6
MS.gene91209.t1 MTR_4g087100 28.659 164 90 4 120 258 179 340 2.89e-13 68.9
MS.gene91209.t1 MTR_8g009120 33.645 107 60 2 164 259 242 348 3.30e-13 68.9
MS.gene91209.t1 MTR_2g083030 37.500 96 46 2 164 251 228 317 3.58e-13 68.6
MS.gene91209.t1 MTR_8g009150 32.143 112 65 2 163 263 233 344 3.87e-13 68.6
MS.gene91209.t1 MTR_4g116010 42.697 89 42 2 164 243 242 330 4.10e-13 68.6
MS.gene91209.t1 MTR_8g009170 35.000 100 54 2 164 252 254 353 4.21e-13 68.6
MS.gene91209.t1 MTR_2g072930 32.308 130 62 4 14 120 14 140 4.49e-13 66.2
MS.gene91209.t1 MTR_1g070120 35.484 93 52 1 164 248 231 323 5.97e-13 68.2
MS.gene91209.t1 MTR_4g024660 31.818 110 61 2 164 259 259 368 7.26e-13 68.2
MS.gene91209.t1 MTR_8g028435 31.776 107 62 2 164 259 253 359 9.96e-13 67.4
MS.gene91209.t1 MTR_5g065010 29.825 171 90 6 120 263 174 341 1.11e-12 67.4
MS.gene91209.t1 MTR_3g091350 34.259 108 62 2 164 263 254 360 1.15e-12 67.4
MS.gene91209.t1 MTR_7g102820 31.429 105 64 1 164 260 272 376 1.85e-12 67.0
MS.gene91209.t1 MTR_5g085330 35.577 104 56 2 164 256 195 298 2.52e-12 66.2
MS.gene91209.t1 MTR_7g102720 31.313 99 60 1 173 263 236 334 3.21e-12 65.9
MS.gene91209.t1 MTR_3g088565 34.653 101 55 2 164 253 195 295 3.37e-12 65.5
MS.gene91209.t1 MTR_2g069010 34.286 105 54 2 164 253 261 365 3.45e-12 65.9
MS.gene91209.t1 MTR_4g117880 36.559 93 48 2 164 245 254 346 3.51e-12 65.9
MS.gene91209.t1 MTR_1g070135 33.333 93 54 1 164 248 231 323 3.75e-12 65.9
MS.gene91209.t1 MTR_2g068840 34.000 100 58 1 172 263 261 360 5.13e-12 65.5
MS.gene91209.t1 MTR_7g016080 39.506 81 49 0 163 243 231 311 5.77e-12 65.1
MS.gene91209.t1 MTR_8g041600 34.821 112 61 2 164 263 262 373 5.94e-12 65.5
MS.gene91209.t1 MTR_1g070080 36.559 93 51 1 164 248 231 323 8.77e-12 64.7
MS.gene91209.t1 MTR_4g132770 35.789 95 51 2 164 248 64 158 9.54e-12 62.0
MS.gene91209.t1 MTR_2g083000 31.818 110 57 3 164 263 223 324 9.89e-12 64.3
MS.gene91209.t1 MTR_8g063070 30.000 110 63 2 164 259 259 368 1.19e-11 64.3
MS.gene91209.t1 MTR_5g005570 38.043 92 43 3 164 247 227 312 1.27e-11 64.3
MS.gene91209.t1 MTR_0032s0040 33.333 108 60 3 164 259 261 368 1.43e-11 64.3
MS.gene91209.t1 MTR_7g029655 32.432 111 59 4 164 259 256 365 2.56e-11 63.5
MS.gene91209.t1 MTR_3g088745 23.154 298 129 9 51 248 37 334 3.05e-11 63.2
MS.gene91209.t1 MTR_8g461330 32.110 109 65 2 164 263 224 332 3.27e-11 62.8
MS.gene91209.t1 MTR_2g069060 35.106 94 52 2 164 248 241 334 4.54e-11 62.8
MS.gene91209.t1 MTR_3g043900 30.693 101 62 1 171 263 252 352 5.39e-11 62.4
MS.gene91209.t1 MTR_4g087120 27.160 162 91 4 120 256 179 338 6.22e-11 62.4
MS.gene91209.t1 MTR_2g068950 33.333 99 51 2 164 247 255 353 8.46e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91209.t1 AT3G21420 46.006 363 94 8 1 263 1 361 8.20e-97 288
MS.gene91209.t1 AT4G25310 30.868 311 149 10 17 263 16 324 2.25e-34 126
MS.gene91209.t1 AT1G78550 28.788 330 140 10 27 263 28 355 9.09e-34 125
MS.gene91209.t1 AT1G17020 28.986 345 143 11 17 263 17 357 2.73e-33 124
MS.gene91209.t1 AT4G25310 28.070 342 152 11 14 263 13 352 5.28e-30 115
MS.gene91209.t1 AT1G17010 29.014 355 151 12 5 263 8 357 5.42e-28 110
MS.gene91209.t1 AT5G05600 25.074 339 151 10 17 256 27 361 3.01e-26 105
MS.gene91209.t1 AT1G78550 29.167 264 113 8 70 263 18 277 1.54e-25 102
MS.gene91209.t1 AT2G38240 25.291 344 155 9 14 260 9 347 3.69e-24 99.8
MS.gene91209.t1 AT3G55970 23.837 344 162 8 17 260 14 357 1.23e-22 95.9
MS.gene91209.t1 AT3G11180 23.977 342 151 10 17 256 56 390 4.87e-20 89.0
MS.gene91209.t1 AT4G25300 40.741 108 56 1 164 263 154 261 1.30e-19 85.9
MS.gene91209.t1 AT3G11180 24.128 344 149 11 17 258 56 389 1.72e-19 87.4
MS.gene91209.t1 AT4G25300 40.741 108 56 1 164 263 248 355 2.91e-19 86.3
MS.gene91209.t1 AT1G77330 41.584 101 51 1 164 256 197 297 1.34e-18 84.0
MS.gene91209.t1 AT4G10500 42.453 106 52 2 164 260 237 342 2.78e-18 83.6
MS.gene91209.t1 AT4G10490 33.645 107 61 2 164 260 235 341 6.26e-17 79.7
MS.gene91209.t1 AT5G20400 24.699 332 149 9 28 262 19 346 1.58e-16 78.6
MS.gene91209.t1 AT3G19010 41.667 108 50 3 164 261 203 307 5.15e-16 76.6
MS.gene91209.t1 AT3G19010 42.056 107 49 3 164 260 233 336 5.62e-16 77.0
MS.gene91209.t1 AT3G19010 42.056 107 49 3 164 260 233 336 5.62e-16 77.0
MS.gene91209.t1 AT5G59530 38.739 111 57 2 164 263 253 363 8.58e-16 76.6
MS.gene91209.t1 AT1G04380 37.838 111 58 2 164 263 234 344 1.50e-15 75.9
MS.gene91209.t1 AT1G12010 38.614 101 52 2 164 256 197 295 1.88e-15 75.1
MS.gene91209.t1 AT5G59540 39.640 111 56 2 164 263 255 365 1.97e-15 75.5
MS.gene91209.t1 AT1G55290 36.697 109 59 2 164 262 251 359 2.25e-15 75.5
MS.gene91209.t1 AT2G30840 40.000 105 55 5 164 261 251 354 1.15e-14 73.2
MS.gene91209.t1 AT1G49390 25.240 313 125 11 49 262 44 346 1.43e-14 72.8
MS.gene91209.t1 AT1G49390 25.362 276 120 9 61 262 4 267 3.98e-14 70.9
MS.gene91209.t1 AT2G30840 37.838 111 55 5 164 261 251 360 7.29e-14 70.9
MS.gene91209.t1 AT1G62380 35.644 101 55 2 164 256 197 295 8.41e-14 70.5
MS.gene91209.t1 AT1G06620 33.929 112 61 3 164 263 254 364 1.48e-13 70.1
MS.gene91209.t1 AT4G16330 35.238 105 56 2 164 260 154 254 1.58e-13 68.9
MS.gene91209.t1 AT1G04350 37.037 108 56 3 164 259 248 355 2.04e-13 69.7
MS.gene91209.t1 AT4G16330 35.238 105 56 2 164 260 260 360 3.20e-13 68.9
MS.gene91209.t1 AT2G30830 36.111 108 56 4 164 259 247 353 3.56e-13 68.9
MS.gene91209.t1 AT3G12900 35.780 109 61 2 164 263 247 355 5.65e-13 68.2
MS.gene91209.t1 AT2G25450 39.474 114 53 5 164 263 247 358 6.32e-13 68.2
MS.gene91209.t1 AT5G54000 25.000 288 118 8 69 262 64 347 8.19e-13 67.8
MS.gene91209.t1 AT5G54000 25.820 244 104 7 92 262 28 267 1.63e-12 66.2
MS.gene91209.t1 AT5G43450 32.710 107 61 2 164 259 251 357 2.27e-12 66.6
MS.gene91209.t1 AT5G43440 34.821 112 60 3 164 263 254 364 2.33e-12 66.6
MS.gene91209.t1 AT3G19000 40.206 97 48 2 158 244 232 328 4.10e-12 65.9
MS.gene91209.t1 AT5G24530 31.765 170 83 7 120 260 171 336 5.15e-12 65.5
MS.gene91209.t1 AT3G13610 30.909 110 66 2 164 263 251 360 2.41e-11 63.5
MS.gene91209.t1 AT1G03410 34.951 103 53 4 164 253 250 351 2.74e-11 63.5
MS.gene91209.t1 AT1G03410 34.951 103 53 4 164 253 287 388 2.75e-11 63.5
MS.gene91209.t1 AT5G08640 36.735 98 53 2 164 253 237 333 3.78e-11 62.8
MS.gene91209.t1 AT5G08640 36.735 98 53 2 164 253 237 333 3.78e-11 62.8
MS.gene91209.t1 AT1G05010 33.962 106 57 3 164 256 194 299 9.87e-11 61.6

Find 52 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACCCATTTGTTGTCTTTA+AGG 0.122767 2.2:+52011764 None:intergenic
AATCTTTGAGGAACTGTCTT+TGG 0.265374 2.2:+52013002 None:intergenic
CCATAACCTTGGACAGTTCC+TGG 0.282406 2.2:+52012306 None:intergenic
GTTGGATAGTAATTCATCTA+TGG 0.324658 2.2:+52011888 None:intergenic
GCATTTGGAATTGGTTCAAT+AGG 0.325952 2.2:+52011742 None:intergenic
CCAGGAACTGTCCAAGGTTA+TGG 0.344847 2.2:-52012306 MS.gene91209:CDS
AAATCCTTAAAGACAACAAA+TGG 0.349073 2.2:-52011768 MS.gene91209:CDS
CATAGCATTAGGTCTGAGTT+TGG 0.349146 2.2:-52011953 MS.gene91209:intron
ATAACAAGAGCATTTGGAAT+TGG 0.381642 2.2:+52011733 None:intergenic
CCTACATTAATGCTAGAAAT+AGG 0.392670 2.2:+52013062 None:intergenic
ATTTCTCTAAGTTTAGTAAA+GGG 0.401195 2.2:-52012914 MS.gene91209:CDS
CATTTGGAATTGGTTCAATA+GGG 0.427746 2.2:+52011743 None:intergenic
TCACTGTGCTGCAGCAGCTA+AGG 0.440862 2.2:-52011809 MS.gene91209:CDS
ATTTCTATCTTGCAAAGTCC+AGG 0.448164 2.2:+52011023 None:intergenic
ATGTTGATAACAAGAGCATT+TGG 0.457793 2.2:+52011727 None:intergenic
ATGTCTCTTACAAATCTTTG+AGG 0.459201 2.2:+52012990 None:intergenic
TGCAACTGCTTGTGAGGAGT+GGG 0.474417 2.2:-52012856 MS.gene91209:CDS
GATTTCTCTAAGTTTAGTAA+AGG 0.477237 2.2:-52012915 MS.gene91209:CDS
TGGACAGTTCCTGGAGCCAT+AGG 0.477708 2.2:+52012315 None:intergenic
AATGCTCTTGTTATCAACAT+TGG 0.480182 2.2:-52011724 MS.gene91209:CDS
TGGACCAATGCTTGAACTTG+TGG 0.483380 2.2:-52011134 MS.gene91209:CDS
CCTAGCTATGAAGTAGAGCT+TGG 0.487986 2.2:-52011154 MS.gene91209:CDS
CAAAAGCTTGTCCATAACCT+TGG 0.501687 2.2:+52012295 None:intergenic
CCTATTTCTAGCATTAATGT+AGG 0.505066 2.2:-52013062 MS.gene91209:CDS
CAAAAGTACCCTATGGCTCC+AGG 0.508802 2.2:-52012324 MS.gene91209:CDS
CACTGTGCTGCAGCAGCTAA+GGG 0.511896 2.2:-52011808 MS.gene91209:CDS
TTAAGGATTTGAAGACCCAC+TGG 0.512276 2.2:+52011781 None:intergenic
CCAAGCTCTACTTCATAGCT+AGG 0.515412 2.2:+52011154 None:intergenic
TCATCCACAAGTTCAAGCAT+TGG 0.522900 2.2:+52011130 None:intergenic
CAAATAAGTTACAAGGAAAG+AGG 0.529031 2.2:-52011048 MS.gene91209:CDS
TAGCTGCTGCAGCACAGTGA+GGG 0.535015 2.2:+52011812 None:intergenic
AATCCTTAAAGACAACAAAT+GGG 0.549367 2.2:-52011767 MS.gene91209:CDS
TAAGCTTGCAACTGCTTGTG+AGG 0.554021 2.2:-52012862 MS.gene91209:CDS
GGACAGTTCCTGGAGCCATA+GGG 0.570669 2.2:+52012316 None:intergenic
GCAGCTAAGGGAAGCCCAGT+GGG 0.571564 2.2:-52011796 MS.gene91209:CDS
GCATAGAGCTGTGGCACATG+AGG 0.573830 2.2:-52011212 MS.gene91209:CDS
AGAAATAGGTACAGGAGCCA+TGG 0.574113 2.2:+52013076 None:intergenic
TTAATGCTAGAAATAGGTAC+AGG 0.576227 2.2:+52013068 None:intergenic
GCAACTGCTTGTGAGGAGTG+GGG 0.576394 2.2:-52012855 MS.gene91209:CDS
TTGCAACTGCTTGTGAGGAG+TGG 0.578464 2.2:-52012857 MS.gene91209:CDS
TTAGCTGCTGCAGCACAGTG+AGG 0.584460 2.2:+52011811 None:intergenic
ATGGCTCCAGGAACTGTCCA+AGG 0.591027 2.2:-52012312 MS.gene91209:CDS
TTTCTATCTTGCAAAGTCCA+GGG 0.597289 2.2:+52011024 None:intergenic
AAGAGAGCAAAAGTACCCTA+TGG 0.599235 2.2:-52012331 MS.gene91209:CDS
TATGTAACAAATAAGTTACA+AGG 0.599251 2.2:-52011055 MS.gene91209:CDS
AACTTAGAGAAATCAATGAC+AGG 0.605130 2.2:+52012924 None:intergenic
AAGTATAAAAGATACAATCA+TGG 0.612751 2.2:-52011094 MS.gene91209:CDS
TAAGGATTTGAAGACCCACT+GGG 0.617052 2.2:+52011782 None:intergenic
GTTTAGTAAAGGGAACAAAG+AGG 0.627457 2.2:-52012904 MS.gene91209:CDS
GAGTGTGGAGCATAGAGCTG+TGG 0.642957 2.2:-52011221 MS.gene91209:CDS
AGCAGCTAAGGGAAGCCCAG+TGG 0.657033 2.2:-52011797 MS.gene91209:CDS
GTTACAAGGAAAGAGGACCC+TGG 0.686635 2.2:-52011041 MS.gene91209:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAAAATTAATTGAAG+AGG + chr2.2:52012436-52012455 None:intergenic 10.0%
!! TTAAAAAAATTAATTGAAGA+GGG + chr2.2:52012435-52012454 None:intergenic 10.0%
!!! TATTATTTTATAATTTGGTA+AGG - chr2.2:52011305-52011324 MS.gene91209:intron 10.0%
!! TTTACCAAGTAAATAATAAA+AGG + chr2.2:52012040-52012059 None:intergenic 15.0%
!!! ATTGATAAAATTTGTTAGAA+AGG - chr2.2:52012000-52012019 MS.gene91209:intron 15.0%
!!! CAAATTTTATCAATTTTGTA+TGG + chr2.2:52011994-52012013 None:intergenic 15.0%
!!! GCTACTATTATTTTATAATT+TGG - chr2.2:52011300-52011319 MS.gene91209:intron 15.0%
!!! TAATCCTTTTATTATTTACT+TGG - chr2.2:52012033-52012052 MS.gene91209:intron 15.0%
!!! TCATTTTCTCAAATTATAAT+AGG + chr2.2:52012533-52012552 None:intergenic 15.0%
!!! TTAATTTTTCATTTTTCTCT+TGG - chr2.2:52012809-52012828 MS.gene91209:intron 15.0%
!! AAGTATAAAAGATACAATCA+TGG - chr2.2:52013007-52013026 MS.gene91209:CDS 20.0%
!! AATTGTACTCAAGAAAAATA+AGG - chr2.2:52012098-52012117 MS.gene91209:intron 20.0%
!! ATTAAGTTATTTATCGAAAC+AGG + chr2.2:52011346-52011365 None:intergenic 20.0%
!! ATTTCTCTAAGTTTAGTAAA+GGG - chr2.2:52011187-52011206 MS.gene91209:CDS 20.0%
!! TATGTAACAAATAAGTTACA+AGG - chr2.2:52013046-52013065 MS.gene91209:CDS 20.0%
!! TGAAAAAGTTAACCATATAT+AGG + chr2.2:52011591-52011610 None:intergenic 20.0%
!!! TAACTTTTTCAAGAAAAACT+TGG - chr2.2:52011600-52011619 MS.gene91209:intron 20.0%
!!! TATGTATTTCAGCAAATTTT+TGG + chr2.2:52012132-52012151 None:intergenic 20.0%
! AAATCCTTAAAGACAACAAA+TGG - chr2.2:52012333-52012352 MS.gene91209:CDS 25.0%
! AATCCTTAAAGACAACAAAT+GGG - chr2.2:52012334-52012353 MS.gene91209:CDS 25.0%
! ATATTAGGAACTCAAATCTT+TGG - chr2.2:52011891-52011910 MS.gene91209:CDS 25.0%
! ATTAAATCACTATGCACAAA+AGG + chr2.2:52012794-52012813 None:intergenic 25.0%
! CATTCAATAAATGATGTATG+TGG - chr2.2:52012619-52012638 MS.gene91209:intron 25.0%
! CATTCAATAAATGATGTATG+TGG + chr2.2:52012638-52012619 None:intergenic 25.0%
! GATTTCTCTAAGTTTAGTAA+AGG - chr2.2:52011186-52011205 MS.gene91209:CDS 25.0%
! TTGGTAAGCTTATGAAAATA+AGG - chr2.2:52011460-52011479 MS.gene91209:intron 25.0%
!! AAGTTTCTTACATTTAAGAC+CGG + chr2.2:52012584-52012603 None:intergenic 25.0%
!! AGTTGTTTCCATAAATTCTT+CGG - chr2.2:52011508-52011527 MS.gene91209:intron 25.0%
!! ATTTTACAGGTTCTTACAAA+TGG - chr2.2:52012848-52012867 MS.gene91209:CDS 25.0%
!! TAATGTTAAGTGTTGTCATT+AGG - chr2.2:52011670-52011689 MS.gene91209:intron 25.0%
!! TGATTCTTTTTGCATTTTAC+AGG - chr2.2:52012835-52012854 MS.gene91209:intron 25.0%
!! TGTATCTTTTATACTTGCAT+GGG + chr2.2:52013003-52013022 None:intergenic 25.0%
!! TTGTATCTTTTATACTTGCA+TGG + chr2.2:52013004-52013023 None:intergenic 25.0%
AAATGGAAAATACAAGAGTG+TGG - chr2.2:52012865-52012884 MS.gene91209:CDS 30.0%
AACTTAGAGAAATCAATGAC+AGG + chr2.2:52011180-52011199 None:intergenic 30.0%
AGAACATAGAAGATATGAGT+AGG - chr2.2:52011726-52011745 MS.gene91209:CDS 30.0%
ATATCAAGCAGTCCTATATA+TGG - chr2.2:52011576-52011595 MS.gene91209:intron 30.0%
ATGTCTCTTACAAATCTTTG+AGG + chr2.2:52011114-52011133 None:intergenic 30.0%
CAAATAAGTTACAAGGAAAG+AGG - chr2.2:52013053-52013072 MS.gene91209:CDS 30.0%
CCTACATTAATGCTAGAAAT+AGG + chr2.2:52011042-52011061 None:intergenic 30.0%
GAACATAGAAGATATGAGTA+GGG - chr2.2:52011727-52011746 MS.gene91209:CDS 30.0%
GAATTGAACCTCACTATATT+AGG - chr2.2:52011876-52011895 MS.gene91209:intron 30.0%
GTTGGATAGTAATTCATCTA+TGG + chr2.2:52012216-52012235 None:intergenic 30.0%
TAACGTTACGTATATCATCT+AGG - chr2.2:52011949-52011968 MS.gene91209:CDS 30.0%
TTAATGCTAGAAATAGGTAC+AGG + chr2.2:52011036-52011055 None:intergenic 30.0%
TTGCTGAAATACATAGCATT+AGG - chr2.2:52012137-52012156 MS.gene91209:intron 30.0%
! AATGCTCTTGTTATCAACAT+TGG - chr2.2:52012377-52012396 MS.gene91209:intron 30.0%
! AGTTTCTTTACATTTGAGAC+AGG - chr2.2:52012694-52012713 MS.gene91209:intron 30.0%
! CATTTGGAATTGGTTCAATA+GGG + chr2.2:52012361-52012380 None:intergenic 30.0%
! TTTGAGTTCCTAATATAGTG+AGG + chr2.2:52011887-52011906 None:intergenic 30.0%
!! ATAACAAGAGCATTTGGAAT+TGG + chr2.2:52012371-52012390 None:intergenic 30.0%
!! ATGTTGATAACAAGAGCATT+TGG + chr2.2:52012377-52012396 None:intergenic 30.0%
!! CCTATTTCTAGCATTAATGT+AGG - chr2.2:52011039-52011058 MS.gene91209:CDS 30.0%
!! GATGTTTTTGAGAAGATGTT+TGG - chr2.2:52012176-52012195 MS.gene91209:intron 30.0%
AATAGGTGTCAACAATGTCT+TGG + chr2.2:52012516-52012535 None:intergenic 35.0%
ATATAATCACATACTCCCTC+CGG - chr2.2:52012562-52012581 MS.gene91209:intron 35.0%
CTATGTTCTCCATCAGATTA+AGG + chr2.2:52011715-52011734 None:intergenic 35.0%
GTTTAGTAAAGGGAACAAAG+AGG - chr2.2:52011197-52011216 MS.gene91209:CDS 35.0%
TATAGTGAGGTTCAATTCCT+AGG + chr2.2:52011874-52011893 None:intergenic 35.0%
TGAGATTGACCTTAATCTGA+TGG - chr2.2:52011703-52011722 MS.gene91209:intron 35.0%
TTTGAAGACATGCTGTTCAT+TGG - chr2.2:52011641-52011660 MS.gene91209:intron 35.0%
! AATCTTTGAGGAACTGTCTT+TGG + chr2.2:52011102-52011121 None:intergenic 35.0%
! ATCTTTTATACTTGCATGGG+TGG + chr2.2:52013000-52013019 None:intergenic 35.0%
! GCATTTGGAATTGGTTCAAT+AGG + chr2.2:52012362-52012381 None:intergenic 35.0%
! TCTTTACATTTGAGACAGGA+GGG - chr2.2:52012698-52012717 MS.gene91209:intron 35.0%
! TGTTGCACCAATCAAGTTTT+TGG + chr2.2:52011845-52011864 None:intergenic 35.0%
! TTCTTACATTTAAGACCGGA+GGG + chr2.2:52012580-52012599 None:intergenic 35.0%
! TTCTTTACATTTGAGACAGG+AGG - chr2.2:52012697-52012716 MS.gene91209:intron 35.0%
! TTTCTTACATTTAAGACCGG+AGG + chr2.2:52012581-52012600 None:intergenic 35.0%
!! GCTGTTTTCATGAGCTATTT+TGG - chr2.2:52011441-52011460 MS.gene91209:intron 35.0%
!!! ACAAGCTTTTGTTTTCTCAG+AGG - chr2.2:52011818-52011837 MS.gene91209:CDS 35.0%
AGACGGTTCCGAAGAATTTA+TGG + chr2.2:52011519-52011538 None:intergenic 40.0%
ATACATTACCTGAGTCTTGC+TGG + chr2.2:52011926-52011945 None:intergenic 40.0%
CAAAAGCTTGTCCATAACCT+TGG + chr2.2:52011809-52011828 None:intergenic 40.0%
CAGAGGACCAAAAACTTGAT+TGG - chr2.2:52011835-52011854 MS.gene91209:intron 40.0%
CATAGCATTAGGTCTGAGTT+TGG - chr2.2:52012148-52012167 MS.gene91209:intron 40.0%
TAAGGATTTGAAGACCCACT+GGG + chr2.2:52012322-52012341 None:intergenic 40.0%
TCATCCACAAGTTCAAGCAT+TGG + chr2.2:52012974-52012993 None:intergenic 40.0%
TTAAGGATTTGAAGACCCAC+TGG + chr2.2:52012323-52012342 None:intergenic 40.0%
TTCCATAAACACCTGTGAGA+CGG + chr2.2:52011536-52011555 None:intergenic 40.0%
! GTACTTTTGCTCTCTTCCAA+AGG + chr2.2:52011766-52011785 None:intergenic 40.0%
!! AAGAGAGCAAAAGTACCCTA+TGG - chr2.2:52011770-52011789 MS.gene91209:CDS 40.0%
!! GGACCCATTTGTTGTCTTTA+AGG + chr2.2:52012340-52012359 None:intergenic 40.0%
!! TTCAAGACCTGACCTTGTTT+TGG - chr2.2:52012239-52012258 MS.gene91209:intron 40.0%
!! TTTTGTGGGGAAAGAAGTGT+TGG + chr2.2:52011147-52011166 None:intergenic 40.0%
!!! TCAAGACCTGACCTTGTTTT+GGG - chr2.2:52012240-52012259 MS.gene91209:intron 40.0%
AACCGTCTCACAGGTGTTTA+TGG - chr2.2:52011531-52011550 MS.gene91209:intron 45.0%
AGGTCAGGTCTTGAACATGT+TGG + chr2.2:52012234-52012253 None:intergenic 45.0%
CCAAGCTCTACTTCATAGCT+AGG + chr2.2:52012950-52012969 None:intergenic 45.0%
CCTAGCTATGAAGTAGAGCT+TGG - chr2.2:52012947-52012966 MS.gene91209:CDS 45.0%
CTCAAACCCAAAACAAGGTC+AGG + chr2.2:52012249-52012268 None:intergenic 45.0%
GAGGACTCAAACCCAAAACA+AGG + chr2.2:52012254-52012273 None:intergenic 45.0%
GGTTTGAGTCCTCATTCAGA+TGG - chr2.2:52012261-52012280 MS.gene91209:intron 45.0%
TAAGCTTGCAACTGCTTGTG+AGG - chr2.2:52011239-52011258 MS.gene91209:intron 45.0%
TGGACCAATGCTTGAACTTG+TGG - chr2.2:52012967-52012986 MS.gene91209:CDS 45.0%
TGGCACATGAGGAAAAAGAC+AGG - chr2.2:52012900-52012919 MS.gene91209:CDS 45.0%
TGTCTCAGGTATGTCAGTGT+CGG + chr2.2:52012737-52012756 None:intergenic 45.0%
TTGGTGCAACATGTTTGCCT+AGG - chr2.2:52011854-52011873 MS.gene91209:intron 45.0%
! ACAGGCATGTCACTGTTTTG+TGG + chr2.2:52011162-52011181 None:intergenic 45.0%
! AGGCATGTCACTGTTTTGTG+GGG + chr2.2:52011160-52011179 None:intergenic 45.0%
! CAGGCATGTCACTGTTTTGT+GGG + chr2.2:52011161-52011180 None:intergenic 45.0%
! CTATGTGATGAGAGCGTGTT+CGG + chr2.2:52012760-52012779 None:intergenic 45.0%
! GGGAGTTTTCATGCTACCTT+TGG - chr2.2:52011747-52011766 MS.gene91209:CDS 45.0%
ATTCTTCGGAACCGTCTCAC+AGG - chr2.2:52011522-52011541 MS.gene91209:intron 50.0%
CAAAGAAGCCAGCAAGACTC+AGG - chr2.2:52011915-52011934 MS.gene91209:CDS 50.0%
CCAGGAACTGTCCAAGGTTA+TGG - chr2.2:52011795-52011814 MS.gene91209:CDS 50.0%
CCATAACCTTGGACAGTTCC+TGG + chr2.2:52011798-52011817 None:intergenic 50.0%
GTTACAAGGAAAGAGGACCC+TGG - chr2.2:52013060-52013079 MS.gene91209:CDS 50.0%
TGCAACTGCTTGTGAGGAGT+GGG - chr2.2:52011245-52011264 MS.gene91209:intron 50.0%
TTGCAACTGCTTGTGAGGAG+TGG - chr2.2:52011244-52011263 MS.gene91209:intron 50.0%
! AGGGCACTTCCATCTGAATG+AGG + chr2.2:52012273-52012292 None:intergenic 50.0%
! GGTCTGTGTCGATGCTTCAT+AGG + chr2.2:52012474-52012493 None:intergenic 50.0%
! GTGAGGAGTGGGGATTTTTC+AGG - chr2.2:52011256-52011275 MS.gene91209:intron 50.0%
!! CAAAAGTACCCTATGGCTCC+AGG - chr2.2:52011777-52011796 MS.gene91209:CDS 50.0%
!! CTGAGTCTTGCTGGCTTCTT+TGG + chr2.2:52011917-52011936 None:intergenic 50.0%
ATGGCTCCAGGAACTGTCCA+AGG - chr2.2:52011789-52011808 MS.gene91209:CDS 55.0%
CACTGTGCTGCAGCAGCTAA+GGG - chr2.2:52012293-52012312 MS.gene91209:CDS 55.0%
GAGTGTGGAGCATAGAGCTG+TGG - chr2.2:52012880-52012899 MS.gene91209:CDS 55.0%
GCAACTGCTTGTGAGGAGTG+GGG - chr2.2:52011246-52011265 MS.gene91209:intron 55.0%
GCATAGAGCTGTGGCACATG+AGG - chr2.2:52012889-52012908 MS.gene91209:CDS 55.0%
GGACAGTTCCTGGAGCCATA+GGG + chr2.2:52011788-52011807 None:intergenic 55.0%
TAGCTGCTGCAGCACAGTGA+GGG + chr2.2:52012292-52012311 None:intergenic 55.0%
TCACTGTGCTGCAGCAGCTA+AGG - chr2.2:52012292-52012311 MS.gene91209:CDS 55.0%
TGGACAGTTCCTGGAGCCAT+AGG + chr2.2:52011789-52011808 None:intergenic 55.0%
TTAGCTGCTGCAGCACAGTG+AGG + chr2.2:52012293-52012312 None:intergenic 55.0%
! CTTGGTGTGAGTGTCGTGTC+TGG + chr2.2:52012498-52012517 None:intergenic 55.0%
AGCAGCTAAGGGAAGCCCAG+TGG - chr2.2:52012304-52012323 MS.gene91209:CDS 60.0%
GCAGCTAAGGGAAGCCCAGT+GGG - chr2.2:52012305-52012324 MS.gene91209:CDS 60.0%
! GGTGTGAGTGTCGTGTCTGG+TGG + chr2.2:52012495-52012514 None:intergenic 60.0%
!! GAGAGCGTGTTCGGTGTCTC+AGG + chr2.2:52012751-52012770 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52011027 52013096 52011027 ID=MS.gene91209
chr2.2 mRNA 52011027 52013096 52011027 ID=MS.gene91209.t1;Parent=MS.gene91209
chr2.2 exon 52012846 52013096 52012846 ID=MS.gene91209.t1.exon1;Parent=MS.gene91209.t1
chr2.2 CDS 52012846 52013096 52012846 ID=cds.MS.gene91209.t1;Parent=MS.gene91209.t1
chr2.2 exon 52012265 52012371 52012265 ID=MS.gene91209.t1.exon2;Parent=MS.gene91209.t1
chr2.2 CDS 52012265 52012371 52012265 ID=cds.MS.gene91209.t1;Parent=MS.gene91209.t1
chr2.2 exon 52011886 52011970 52011886 ID=MS.gene91209.t1.exon3;Parent=MS.gene91209.t1
chr2.2 CDS 52011886 52011970 52011886 ID=cds.MS.gene91209.t1;Parent=MS.gene91209.t1
chr2.2 exon 52011711 52011843 52011711 ID=MS.gene91209.t1.exon4;Parent=MS.gene91209.t1
chr2.2 CDS 52011711 52011843 52011711 ID=cds.MS.gene91209.t1;Parent=MS.gene91209.t1
chr2.2 exon 52011027 52011242 52011027 ID=MS.gene91209.t1.exon5;Parent=MS.gene91209.t1
chr2.2 CDS 52011027 52011242 52011027 ID=cds.MS.gene91209.t1;Parent=MS.gene91209.t1
Gene Sequence

>MS.gene91209

ATGGCTCCTGTACCTATTTCTAGCATTAATGTAGGACACATTGATGATGTACAAGAATTAAGAAGAACTAAGCCAAAGACAGTTCCTCAAAGATTTGTAAGAGACATGACAGAAAGACCAACACTTCTTTCCCCACAAAACAGTGACATGCCTGTCATTGATTTCTCTAAGTTTAGTAAAGGGAACAAAGAGGAAGTTTTGAATGAACTTTCTAAGCTTGCAACTGCTTGTGAGGAGTGGGGATTTTTCAGGTAAATGTTAATAATCTCTTAAGCTACTATTATTTTATAATTTGGTAAGGTTCATTAACTTAAGTCCTGTTTCGATAAATAACTTAATTAAGCGCTTATGCATAAGTTGTTTCGATAACAAAATATAAATAAAATAAAGTCAAACTGATTTCATAAGTTATAAGCTGTTTTCATGAGCTATTTTGGTAAGCTTATGAAAATAAGGTGAAACAACTTGTGAACATGTCATAAGTTGTTTCCATAAATTCTTCGGAACCGTCTCACAGGTGTTTATGGAATTAAATAAGCTCAAATAAGTATATCAAGCAGTCCTATATATGGTTAACTTTTTCAAGAAAAACTTGGTATAGTTAACATAGATTTTTTGAAGACATGCTGTTCATTGGAATTAATAATGTTAAGTGTTGTCATTAGGTGATTAATCATGAGATTGACCTTAATCTGATGGAGAACATAGAAGATATGAGTAGGGAGTTTTCATGCTACCTTTGGAAGAGAGCAAAAGTACCCTATGGCTCCAGGAACTGTCCAAGGTTATGGACAAGCTTTTGTTTTCTCAGAGGACCAAAAACTTGATTGGTGCAACATGTTTGCCTAGGAATTGAACCTCACTATATTAGGAACTCAAATCTTTGGCCAAAGAAGCCAGCAAGACTCAGGTAATGTATATGTAACGTTACGTATATCATCTAGGAACAAATATGATTTTCTGTCCATACAAAATTGATAAAATTTGTTAGAAAGGTATATGTGTATAATCCTTTTATTATTTACTTGGTAAAATGATTATTTTTTTCATACTCATGCAGTGAAACAATTGAATTGTACTCAAGAAAAATAAGGAAACTCTGCCAAAAATTTGCTGAAATACATAGCATTAGGTCTGAGTTTGGAAGAAGATGTTTTTGAGAAGATGTTTGGTGAAGCAGTACAAGCCATAGATGAATTACTATCCAACATGTTCAAGACCTGACCTTGTTTTGGGTTTGAGTCCTCATTCAGATGGAAGTGCCCTCACTGTGCTGCAGCAGCTAAGGGAAGCCCAGTGGGTCTTCAAATCCTTAAAGACAACAAATGGGTCCCTATTGAACCAATTCCAAATGCTCTTGTTATCAACATTGGAGACACAATAGAAGTAAGACTACTATTTTCTTCCCTCTTCAATTAATTTTTTTAATTTTAATTACTATATACCTATGAAGCATCGACACAGACCACCAGACACGACACTCACACCAAGACATTGTTGACACCTATTATAATTTGAGAAAATGAACTAATTGAATATAATCACATACTCCCTCCGGTCTTAAATGTAAGAAACTTTTCACTTTTTTAGTTCATTCAATAAATGATGTATGTGGTCTATAATAAAGACCACATACATCATTTATTGAATGAATCTAAAAAGTGAAAAGTTTCTTTACATTTGAGACAGGAGGGAGTATGTTAATATCCGACACTGACATACCTGAGACACCGAACACGCTCTCATCACATAGATATTTGAACACCTTTTGTGCATAGTGATTTAATTTTTCATTTTTCTCTTGGACTTGATTCTTTTTGCATTTTACAGGTTCTTACAAATGGAAAATACAAGAGTGTGGAGCATAGAGCTGTGGCACATGAGGAAAAAGACAGGCTCTCAATTGTGACATTTTATGCTCCTAGCTATGAAGTAGAGCTTGGACCAATGCTTGAACTTGTGGATGAAAACCACCCATGCAAGTATAAAAGATACAATCATGGAGAGTATAGTAAACACTATGTAACAAATAAGTTACAAGGAAAGAGGACCCTGGACTTTGCAAGATAG
Protein sequence

>MS.gene91209.t1

MAPVPISSINVGHIDDVQELRRTKPKTVPQRFVRDMTERPTLLSPQNSDMPVIDFSKFSKGNKEEVLNELSKLATACEEWGFFSFHATFGREQKYPMAPGTVQGYGQAFVFSEDQKLDWSLGLSLEEDVFEKMFGEAVQAIDELLSNIWKCPHCAAAAKGSPVGLQILKDNKWVPIEPIPNALVINIGDTIESVEHRAVAHEEKDRLSIVTFYAPSYEVELGPMLELVDENHPCKYKRYNHGEYSKHYVTNKLQGKRTLDFAR