Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91318.t1 | XP_013469974.1 | 82.7 | 196 | 29 | 4 | 1 | 191 | 3 | 198 | 6.60E-82 | 313.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91318.t1 | A0A072VQE3 | 82.7 | 196 | 29 | 4 | 1 | 191 | 3 | 198 | 4.8e-82 | 313.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053581 | MS.gene91318 | 0.811954 | 5.41E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91318.t1 | MTR_1g106080 | 92.857 | 196 | 9 | 4 | 1 | 191 | 3 | 198 | 1.70e-119 | 337 |
| MS.gene91318.t1 | MTR_5g005230 | 51.020 | 196 | 71 | 11 | 1 | 187 | 1 | 180 | 1.70e-42 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91318.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 1.19e-20 | 84.7 |
| MS.gene91318.t1 | AT4G33800 | 38.953 | 172 | 82 | 8 | 9 | 169 | 5 | 164 | 1.19e-20 | 84.7 |
Find 29 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTTCTGATGCTTCTTC+TGG | 0.266605 | 1.3:-72842233 | MS.gene91318:CDS |
| TCATACCAGGAAGAACAATT+TGG | 0.315479 | 1.3:-72841718 | MS.gene91318:CDS |
| ACAAAGTTATGTAGGAAATA+TGG | 0.330154 | 1.3:-72841880 | MS.gene91318:CDS |
| TATTTCCTACATAACTTTGT+TGG | 0.375448 | 1.3:+72841883 | None:intergenic |
| TGTAGTAGTGGATGTGAGTC+AGG | 0.376527 | 1.3:-72842386 | MS.gene91318:CDS |
| AGGAGTGGAAGATTTGTCTA+TGG | 0.397750 | 1.3:-72842252 | MS.gene91318:CDS |
| GGTGGAACTGAAGGGTACTA+TGG | 0.403947 | 1.3:-72842317 | MS.gene91318:CDS |
| TTATGAAAACACTAGATTCA+TGG | 0.403958 | 1.3:-72842339 | MS.gene91318:CDS |
| AAATTGTTCTTCCTGGTATG+AGG | 0.406008 | 1.3:+72841720 | None:intergenic |
| TCTTTGTTTAGCAGCTGGTA+TGG | 0.422441 | 1.3:-72841563 | MS.gene91318:intron |
| TATGAAAACACTAGATTCAT+GGG | 0.491230 | 1.3:-72842338 | MS.gene91318:CDS |
| GCAGTGTCATGAAGAAAAGA+AGG | 0.507146 | 1.3:+72842077 | None:intergenic |
| GGTACAAAGAAACAGAAAGT+TGG | 0.512053 | 1.3:-72842140 | MS.gene91318:CDS |
| ACAAACCAACAAAGTTATGT+AGG | 0.515352 | 1.3:-72841888 | MS.gene91318:CDS |
| TTGGTTTGTCCCAACAACAT+TGG | 0.519643 | 1.3:+72841902 | None:intergenic |
| AGATTCATGGGTGGAACTGA+AGG | 0.520572 | 1.3:-72842326 | MS.gene91318:CDS |
| ACATCATCATGTGGAAGATG+TGG | 0.558107 | 1.3:+72842206 | None:intergenic |
| GTCACAAATGAGTATTCTGA+AGG | 0.574749 | 1.3:-72842272 | MS.gene91318:CDS |
| AAATGAGTATTCTGAAGGAG+TGG | 0.595088 | 1.3:-72842267 | MS.gene91318:CDS |
| TTGTTTAGCAGCTGGTATGG+AGG | 0.596105 | 1.3:-72841560 | MS.gene91318:intron |
| TTTGATTCTGAATGTAGTAG+TGG | 0.600879 | 1.3:-72842398 | MS.gene91318:CDS |
| GTAGGGAAAATCCTCATACC+AGG | 0.622430 | 1.3:-72841731 | MS.gene91318:intron |
| TCATCATGTGGAAGATGTGG+AGG | 0.636849 | 1.3:+72842209 | None:intergenic |
| TTGAAGTAAACATCATCATG+TGG | 0.637487 | 1.3:+72842197 | None:intergenic |
| GATTCATGGGTGGAACTGAA+GGG | 0.637517 | 1.3:-72842325 | MS.gene91318:CDS |
| GTAGTGGATGTGAGTCAGGT+TGG | 0.642732 | 1.3:-72842382 | MS.gene91318:CDS |
| TGTCATGAAGAAAAGAAGGA+AGG | 0.667075 | 1.3:+72842081 | None:intergenic |
| GAAAACACTAGATTCATGGG+TGG | 0.698883 | 1.3:-72842335 | MS.gene91318:CDS |
| AATATGGTAGACTATTCACA+AGG | 0.724521 | 1.3:-72841864 | MS.gene91318:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGTTTGTGTTATTTGT+AGG | - | chr1.3:72842199-72842218 | MS.gene91318:CDS | 20.0% |
| !!! | ACTAATTATTTTGAGGTAAT+TGG | - | chr1.3:72842117-72842136 | MS.gene91318:CDS | 20.0% |
| !!! | ATTTGTTTGTGTTATTTGTA+GGG | - | chr1.3:72842200-72842219 | MS.gene91318:CDS | 20.0% |
| ! | ACAAAGTTATGTAGGAAATA+TGG | - | chr1.3:72842068-72842087 | MS.gene91318:CDS | 25.0% |
| ! | TATGAAAACACTAGATTCAT+GGG | - | chr1.3:72841610-72841629 | MS.gene91318:intron | 25.0% |
| ! | TATTTCCTACATAACTTTGT+TGG | + | chr1.3:72842068-72842087 | None:intergenic | 25.0% |
| ! | TTATGAAAACACTAGATTCA+TGG | - | chr1.3:72841609-72841628 | MS.gene91318:intron | 25.0% |
| !! | CTTTTCAACTGTAAGAAATA+TGG | - | chr1.3:72841912-72841931 | MS.gene91318:intron | 25.0% |
| !! | TGTTTTCATAAGAATGTTCA+AGG | + | chr1.3:72841600-72841619 | None:intergenic | 25.0% |
| !!! | TTCAGCAACTAATTATTTTG+AGG | - | chr1.3:72842110-72842129 | MS.gene91318:CDS | 25.0% |
| AAAAACCAAATTGTTCTTCC+TGG | + | chr1.3:72842238-72842257 | None:intergenic | 30.0% | |
| AATATGGTAGACTATTCACA+AGG | - | chr1.3:72842084-72842103 | MS.gene91318:CDS | 30.0% | |
| ACAAACCAACAAAGTTATGT+AGG | - | chr1.3:72842060-72842079 | MS.gene91318:CDS | 30.0% | |
| CTCTCAAAAAATGAACTTCA+AGG | - | chr1.3:72842269-72842288 | MS.gene91318:CDS | 30.0% | |
| TTGAAGTAAACATCATCATG+TGG | + | chr1.3:72841754-72841773 | None:intergenic | 30.0% | |
| TTTGATTCTGAATGTAGTAG+TGG | - | chr1.3:72841550-72841569 | MS.gene91318:CDS | 30.0% | |
| !! | ATTTTTCAGACCAATGTTGT+TGG | - | chr1.3:72842036-72842055 | MS.gene91318:intron | 30.0% |
| !! | TTTTTCAGACCAATGTTGTT+GGG | - | chr1.3:72842037-72842056 | MS.gene91318:intron | 30.0% |
| !!! | AGTTCATTTTTTGAGAGAGA+TGG | + | chr1.3:72842266-72842285 | None:intergenic | 30.0% |
| AAATTGTTCTTCCTGGTATG+AGG | + | chr1.3:72842231-72842250 | None:intergenic | 35.0% | |
| GGTACAAAGAAACAGAAAGT+TGG | - | chr1.3:72841808-72841827 | MS.gene91318:intron | 35.0% | |
| GTCACAAATGAGTATTCTGA+AGG | - | chr1.3:72841676-72841695 | MS.gene91318:CDS | 35.0% | |
| TCATACCAGGAAGAACAATT+TGG | - | chr1.3:72842230-72842249 | MS.gene91318:CDS | 35.0% | |
| TGTCATGAAGAAAAGAAGGA+AGG | + | chr1.3:72841870-72841889 | None:intergenic | 35.0% | |
| ! | AAATGAGTATTCTGAAGGAG+TGG | - | chr1.3:72841681-72841700 | MS.gene91318:CDS | 35.0% |
| ! | GTAGTGAAAAAGCACAAGAA+AGG | - | chr1.3:72841787-72841806 | MS.gene91318:intron | 35.0% |
| ! | TGACTCTTTTGTCTTTGTGT+TGG | + | chr1.3:72841660-72841679 | None:intergenic | 35.0% |
| ! | TTCATTCTTTGTTTAGCAGC+TGG | - | chr1.3:72842380-72842399 | MS.gene91318:CDS | 35.0% |
| ACATCATCATGTGGAAGATG+TGG | + | chr1.3:72841745-72841764 | None:intergenic | 40.0% | |
| AGGAGTGGAAGATTTGTCTA+TGG | - | chr1.3:72841696-72841715 | MS.gene91318:CDS | 40.0% | |
| ATGGTTTCTGATGCTTCTTC+TGG | - | chr1.3:72841715-72841734 | MS.gene91318:CDS | 40.0% | |
| GAAAACACTAGATTCATGGG+TGG | - | chr1.3:72841613-72841632 | MS.gene91318:intron | 40.0% | |
| GCAGTGTCATGAAGAAAAGA+AGG | + | chr1.3:72841874-72841893 | None:intergenic | 40.0% | |
| ! | TCTTTGTTTAGCAGCTGGTA+TGG | - | chr1.3:72842385-72842404 | MS.gene91318:CDS | 40.0% |
| !! | TTGGTTTGTCCCAACAACAT+TGG | + | chr1.3:72842049-72842068 | None:intergenic | 40.0% |
| AGATTCATGGGTGGAACTGA+AGG | - | chr1.3:72841622-72841641 | MS.gene91318:intron | 45.0% | |
| GATTCATGGGTGGAACTGAA+GGG | - | chr1.3:72841623-72841642 | MS.gene91318:intron | 45.0% | |
| GTAGGGAAAATCCTCATACC+AGG | - | chr1.3:72842217-72842236 | MS.gene91318:CDS | 45.0% | |
| TCATCATGTGGAAGATGTGG+AGG | + | chr1.3:72841742-72841761 | None:intergenic | 45.0% | |
| TTGTTTAGCAGCTGGTATGG+AGG | - | chr1.3:72842388-72842407 | MS.gene91318:CDS | 45.0% | |
| !! | TGTAGTAGTGGATGTGAGTC+AGG | - | chr1.3:72841562-72841581 | MS.gene91318:intron | 45.0% |
| !! | GGTGGAACTGAAGGGTACTA+TGG | - | chr1.3:72841631-72841650 | MS.gene91318:intron | 50.0% |
| !! | GTAGTGGATGTGAGTCAGGT+TGG | - | chr1.3:72841566-72841585 | MS.gene91318:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 72841541 | 72842429 | 72841541 | ID=MS.gene91318 |
| chr1.3 | mRNA | 72841541 | 72842429 | 72841541 | ID=MS.gene91318.t1;Parent=MS.gene91318 |
| chr1.3 | exon | 72842049 | 72842429 | 72842049 | ID=MS.gene91318.t1.exon1;Parent=MS.gene91318.t1 |
| chr1.3 | CDS | 72842049 | 72842429 | 72842049 | ID=cds.MS.gene91318.t1;Parent=MS.gene91318.t1 |
| chr1.3 | exon | 72841839 | 72841925 | 72841839 | ID=MS.gene91318.t1.exon2;Parent=MS.gene91318.t1 |
| chr1.3 | CDS | 72841839 | 72841925 | 72841839 | ID=cds.MS.gene91318.t1;Parent=MS.gene91318.t1 |
| chr1.3 | exon | 72841673 | 72841749 | 72841673 | ID=MS.gene91318.t1.exon3;Parent=MS.gene91318.t1 |
| chr1.3 | CDS | 72841673 | 72841749 | 72841673 | ID=cds.MS.gene91318.t1;Parent=MS.gene91318.t1 |
| chr1.3 | exon | 72841541 | 72841571 | 72841541 | ID=MS.gene91318.t1.exon4;Parent=MS.gene91318.t1 |
| chr1.3 | CDS | 72841541 | 72841571 | 72841541 | ID=cds.MS.gene91318.t1;Parent=MS.gene91318.t1 |
| Gene Sequence |
| Protein sequence |
