AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91989

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91989.t1 MTR_8g068690 94.400 125 7 0 4 128 154 278 4.25e-84 247
MS.gene91989.t1 MTR_8g068690 94.400 125 7 0 4 128 154 278 2.60e-83 248
MS.gene91989.t1 MTR_8g068750 89.600 125 13 0 4 128 155 279 6.21e-81 242
MS.gene91989.t1 MTR_7g118320 84.000 125 20 0 4 128 140 264 3.03e-76 230
MS.gene91989.t1 MTR_0591s0010 87.200 125 16 0 4 128 155 279 4.17e-76 230
MS.gene91989.t1 MTR_8g068600 85.600 125 18 0 4 128 129 253 1.22e-73 221
MS.gene91989.t1 MTR_6g078850 79.200 125 26 0 4 128 161 285 7.26e-71 220
MS.gene91989.t1 MTR_8g068870 80.800 125 24 0 4 128 141 265 2.64e-70 215
MS.gene91989.t1 MTR_8g068620 86.364 110 15 0 19 128 1 110 2.17e-65 194
MS.gene91989.t1 MTR_8g024160 70.400 125 37 0 4 128 106 230 3.12e-61 191
MS.gene91989.t1 MTR_7g118300 70.400 125 37 0 4 128 153 277 2.17e-60 190
MS.gene91989.t1 MTR_8g068570 78.761 113 24 0 16 128 140 252 4.54e-59 185
MS.gene91989.t1 MTR_3g092900 62.602 123 46 0 6 128 143 265 2.54e-52 169
MS.gene91989.t1 MTR_5g098170 59.350 123 50 0 6 128 143 265 2.88e-49 161
MS.gene91989.t1 MTR_8g068740 66.129 124 10 1 5 128 156 247 1.20e-48 157
MS.gene91989.t1 MTR_8g068730 66.129 124 10 1 5 128 156 247 4.91e-48 157
MS.gene91989.t1 MTR_3g021430 58.036 112 47 0 17 128 149 260 7.12e-41 139
MS.gene91989.t1 MTR_3g021440 58.716 109 45 0 20 128 165 273 1.07e-39 136
MS.gene91989.t1 MTR_1g096310 53.571 112 52 0 17 128 163 274 4.91e-38 132
MS.gene91989.t1 MTR_7g011990 53.571 112 52 0 17 128 158 269 2.60e-37 130
MS.gene91989.t1 MTR_1g036510 51.786 112 54 0 17 128 174 285 4.06e-37 128
MS.gene91989.t1 MTR_7g012070 50.781 128 62 1 2 128 150 277 6.28e-37 129
MS.gene91989.t1 MTR_4g038440 48.031 127 62 2 6 128 144 270 1.41e-36 125
MS.gene91989.t1 MTR_7g012090 50.781 128 62 1 2 128 150 277 1.71e-36 128
MS.gene91989.t1 MTR_1g036510 51.786 112 54 0 17 128 174 285 1.96e-36 128
MS.gene91989.t1 MTR_7g012020 50.893 112 55 0 17 128 160 271 2.70e-36 125
MS.gene91989.t1 MTR_1g036490 50.893 112 55 0 17 128 160 271 4.10e-36 127
MS.gene91989.t1 MTR_7g012020 50.893 112 55 0 17 128 160 271 4.48e-36 127
MS.gene91989.t1 MTR_1g036460 50.000 112 56 0 17 128 160 271 7.41e-36 126
MS.gene91989.t1 MTR_0079s0070 48.031 127 62 2 6 128 110 236 1.20e-35 125
MS.gene91989.t1 MTR_4g038440 48.031 127 62 2 6 128 144 270 1.86e-35 125
MS.gene91989.t1 MTR_2g055940 46.429 112 60 0 17 128 154 265 8.10e-35 123
MS.gene91989.t1 MTR_7g012040 50.893 112 55 0 17 128 159 270 8.64e-35 123
MS.gene91989.t1 MTR_0017s0100 46.457 127 64 2 6 128 144 270 2.41e-34 122
MS.gene91989.t1 MTR_3g096050 48.246 114 58 1 16 128 146 259 3.40e-33 117
MS.gene91989.t1 MTR_7g011900 47.321 112 59 0 17 128 161 272 1.13e-32 118
MS.gene91989.t1 MTR_3g096050 48.246 114 58 1 16 128 146 259 1.84e-32 117
MS.gene91989.t1 MTR_2g072790 46.491 114 60 1 16 128 142 255 6.85e-30 110
MS.gene91989.t1 MTR_3g096070 46.491 114 60 1 16 128 123 236 4.90e-28 105
MS.gene91989.t1 MTR_3g083620 45.299 117 63 1 13 128 145 261 1.45e-26 101
MS.gene91989.t1 MTR_7g012020 46.237 93 50 0 17 109 160 252 2.93e-25 96.3
MS.gene91989.t1 MTR_1g036510 47.253 91 48 0 17 107 174 264 4.63e-25 96.3
MS.gene91989.t1 MTR_5g074600 38.938 113 68 1 17 128 146 258 3.03e-23 92.8
MS.gene91989.t1 MTR_5g074680 38.938 113 68 1 17 128 147 259 1.38e-22 90.9
MS.gene91989.t1 MTR_5g074540 37.168 113 70 1 17 128 128 240 1.61e-19 82.4
MS.gene91989.t1 MTR_5g074580 35.398 113 72 1 17 128 147 259 3.12e-19 82.0
MS.gene91989.t1 MTR_7g118310 44.554 101 40 2 24 108 2 102 4.73e-19 76.6
MS.gene91989.t1 MTR_4g079160 38.532 109 66 1 21 128 147 255 1.87e-18 79.7
MS.gene91989.t1 MTR_1g050220 56.667 60 26 0 60 119 148 207 7.53e-17 74.7
MS.gene91989.t1 MTR_7g014570 32.000 125 84 1 5 128 129 253 1.31e-16 74.7
MS.gene91989.t1 MTR_7g024500 33.945 109 72 0 20 128 142 250 1.43e-16 74.3
MS.gene91989.t1 MTR_1g076930 32.407 108 72 1 22 128 130 237 3.73e-16 72.0
MS.gene91989.t1 MTR_1g076940 30.252 119 80 2 13 128 144 262 6.22e-15 70.1
MS.gene91989.t1 MTR_7g014510 29.032 124 87 1 6 128 135 258 2.26e-14 68.2
MS.gene91989.t1 MTR_7g024520 36.559 93 59 0 36 128 158 250 3.55e-14 67.8
MS.gene91989.t1 MTR_4g033150 33.333 114 74 1 17 128 150 263 4.04e-14 67.8
MS.gene91989.t1 MTR_6g060280 51.852 54 21 1 80 128 1 54 8.70e-14 62.0
MS.gene91989.t1 MTR_4g033015 32.456 114 75 1 17 128 150 263 1.36e-13 66.2
MS.gene91989.t1 MTR_4g088340 33.010 103 69 0 26 128 131 233 1.70e-13 65.9
MS.gene91989.t1 MTR_4g033200 31.579 114 76 1 17 128 150 263 1.04e-12 63.5
MS.gene91989.t1 MTR_4g088200 31.579 114 76 1 17 128 148 261 1.96e-12 62.8
MS.gene91989.t1 MTR_4g033085 31.193 109 73 1 22 128 149 257 5.72e-12 61.6
MS.gene91989.t1 MTR_4g033170 31.193 109 73 1 22 128 149 257 6.00e-12 61.6
MS.gene91989.t1 MTR_4g088345 31.068 103 71 0 26 128 154 256 6.29e-12 61.6
MS.gene91989.t1 MTR_5g074610 39.706 68 41 0 61 128 159 226 8.63e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91989.t1 AT5G54160 61.789 123 47 0 6 128 141 263 1.02e-52 170
MS.gene91989.t1 AT1G77520 55.200 125 56 0 4 128 158 282 1.71e-43 146
MS.gene91989.t1 AT5G37170 53.175 126 56 2 4 128 12 135 2.66e-39 132
MS.gene91989.t1 AT1G33030 50.806 124 60 1 6 128 129 252 1.03e-38 131
MS.gene91989.t1 AT1G33030 50.806 124 60 1 6 128 129 252 1.10e-38 131
MS.gene91989.t1 AT5G37170 53.175 126 56 2 4 128 112 235 2.54e-38 132
MS.gene91989.t1 AT1G51990 48.413 126 64 1 4 128 138 263 5.05e-38 132
MS.gene91989.t1 AT1G33030 50.806 124 60 1 6 128 129 252 9.58e-38 130
MS.gene91989.t1 AT5G53810 57.724 123 52 0 6 128 157 279 9.72e-38 131
MS.gene91989.t1 AT1G51990 47.581 124 64 1 6 128 140 263 1.78e-37 130
MS.gene91989.t1 AT1G77530 56.000 125 55 0 4 128 158 282 2.85e-37 130
MS.gene91989.t1 AT1G63140 54.472 123 56 0 6 128 180 302 3.51e-36 125
MS.gene91989.t1 AT1G63140 54.472 123 56 0 6 128 160 282 1.22e-35 126
MS.gene91989.t1 AT1G63140 54.472 123 56 0 6 128 180 302 1.49e-35 126
MS.gene91989.t1 AT5G37170 62.069 87 32 1 43 128 1 87 9.81e-33 114
MS.gene91989.t1 AT1G62900 55.238 105 47 0 24 128 2 106 7.47e-32 112
MS.gene91989.t1 AT1G76790 52.000 125 57 2 5 128 144 266 1.58e-30 112
MS.gene91989.t1 AT1G21120 51.587 126 58 2 4 128 199 322 3.44e-30 112
MS.gene91989.t1 AT1G21120 51.587 126 58 2 4 128 151 274 4.01e-30 111
MS.gene91989.t1 AT1G21100 52.381 126 57 2 4 128 151 274 4.27e-30 111
MS.gene91989.t1 AT1G21130 50.794 126 59 2 4 128 151 274 6.81e-30 109
MS.gene91989.t1 AT1G21130 50.794 126 59 2 4 128 151 274 1.71e-29 109
MS.gene91989.t1 AT1G21110 50.000 126 60 2 4 128 151 274 6.14e-29 108
MS.gene91989.t1 AT3G53140 44.737 114 62 1 16 128 147 260 4.70e-27 103
MS.gene91989.t1 AT1G77530 50.000 106 53 0 4 109 158 263 6.00e-25 95.9
MS.gene91989.t1 AT1G63140 50.000 98 49 0 6 103 180 277 3.79e-22 89.0
MS.gene91989.t1 AT4G35150 39.091 110 66 1 20 128 109 218 4.87e-20 83.6
MS.gene91989.t1 AT4G35160 41.228 114 66 1 16 128 162 275 1.42e-18 80.1

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCTTCATAACTATTT+TGG 0.151738 3.2:+37339602 None:intergenic
ATAAAGATTGCATCTCCTTT+AGG 0.274360 3.2:+37339180 None:intergenic
GTTGATGTTGGAGGTGGTCT+TGG 0.307601 3.2:-37339540 MS.gene91989:CDS
ATGGTTTCAAGGACATAAAA+AGG 0.340214 3.2:-37339566 MS.gene91989:CDS
ATAATTGTTTCTTCATGCTT+AGG 0.389264 3.2:-37339755 None:intergenic
TGAGAGCTACAATGGTTTCA+AGG 0.426722 3.2:-37339577 MS.gene91989:CDS
CAACATGCCCCTTCGTATCC+TGG 0.434446 3.2:-37339444 MS.gene91989:intron
GTCCCATTCAACATGGTTCA+TGG 0.443754 3.2:-37339699 MS.gene91989:CDS
TTGATGTTGGAGGTGGTCTT+GGG 0.450539 3.2:-37339539 MS.gene91989:CDS
TGCGTTTGATTATGCAAGCT+TGG 0.452740 3.2:-37339670 MS.gene91989:CDS
TACCAAAATAGTTATGAAGA+AGG 0.461453 3.2:-37339604 MS.gene91989:CDS
TCATGGCTGTATTGAAAACT+TGG 0.476007 3.2:+37339634 None:intergenic
AAATTGATACCATGTATATG+AGG 0.480581 3.2:+37339486 None:intergenic
ATTATGCAAGCTTGGACTCA+AGG 0.492297 3.2:-37339662 MS.gene91989:CDS
TGCCATGAACCATGTTGAAT+GGG 0.522273 3.2:+37339697 None:intergenic
TCGAATTGTATTCTTAAGCT+CGG 0.529229 3.2:+37339731 None:intergenic
AGGAGATGCAATCTTTATGA+AGG 0.551013 3.2:-37339175 None:intergenic
TTAAGAATACAATTCGAGAA+GGG 0.552158 3.2:-37339725 MS.gene91989:CDS
GTGCCATGAACCATGTTGAA+TGG 0.556197 3.2:+37339696 None:intergenic
AAGGTTCTTGAGAGCTACAA+TGG 0.560355 3.2:-37339585 MS.gene91989:CDS
AAAAGGCTTGTTGATGTTGG+AGG 0.582099 3.2:-37339549 MS.gene91989:CDS
GTAGGAGTTGAACACGTTGG+CGG 0.587106 3.2:-37339222 MS.gene91989:intron
CTTAAGAATACAATTCGAGA+AGG 0.594263 3.2:-37339726 MS.gene91989:CDS
AATTGATACCATGTATATGA+GGG 0.599306 3.2:+37339487 None:intergenic
TCAAAACACCCTCATATACA+TGG 0.601896 3.2:-37339495 MS.gene91989:CDS
TAAGAATACAATTCGAGAAG+GGG 0.607299 3.2:-37339724 MS.gene91989:CDS
ATGTTTGAAAGTGTACCTAA+AGG 0.613827 3.2:-37339195 MS.gene91989:CDS
AGGCTTGTTGATGTTGGAGG+TGG 0.616259 3.2:-37339546 MS.gene91989:CDS
AGATCATACCAGGATACGAA+GGG 0.620355 3.2:+37339436 None:intergenic
AAGAATACAATTCGAGAAGG+GGG 0.659327 3.2:-37339723 MS.gene91989:CDS
AAGATCATACCAGGATACGA+AGG 0.663878 3.2:+37339435 None:intergenic
TTGGTAGGAGTTGAACACGT+TGG 0.666569 3.2:-37339225 MS.gene91989:intron
GGGGCATGTTGAATGACATG+AGG 0.679220 3.2:+37339456 None:intergenic
GATCATACCAGGATACGAAG+GGG 0.731305 3.2:+37339437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAAAACTATTTAATGT+TGG - chr3.2:37339673-37339692 MS.gene91989:CDS 15.0%
!! TTACATATAAATAAGTAATG+TGG - chr3.2:37339572-37339591 MS.gene91989:CDS 15.0%
!! AAAAACTATTTAATGTTGGT+AGG - chr3.2:37339677-37339696 MS.gene91989:CDS 20.0%
!!! TATTTTGGTATAATTGATCA+TGG + chr3.2:37339303-37339322 None:intergenic 20.0%
! AAATTGATACCATGTATATG+AGG + chr3.2:37339434-37339453 None:intergenic 25.0%
! AATTGATACCATGTATATGA+GGG + chr3.2:37339433-37339452 None:intergenic 25.0%
! TACCAAAATAGTTATGAAGA+AGG - chr3.2:37339313-37339332 MS.gene91989:intron 25.0%
! TTAAGAATACAATTCGAGAA+GGG - chr3.2:37339192-37339211 MS.gene91989:CDS 25.0%
!! AACCTTCTTCATAACTATTT+TGG + chr3.2:37339318-37339337 None:intergenic 25.0%
!!! AACATTTTAAAGATCATACC+AGG + chr3.2:37339494-37339513 None:intergenic 25.0%
ATAAAGATTGCATCTCCTTT+AGG + chr3.2:37339740-37339759 None:intergenic 30.0%
ATGGTTTCAAGGACATAAAA+AGG - chr3.2:37339351-37339370 MS.gene91989:intron 30.0%
CTTAAGAATACAATTCGAGA+AGG - chr3.2:37339191-37339210 MS.gene91989:CDS 30.0%
TAAGAATACAATTCGAGAAG+GGG - chr3.2:37339193-37339212 MS.gene91989:CDS 30.0%
TCGAATTGTATTCTTAAGCT+CGG + chr3.2:37339189-37339208 None:intergenic 30.0%
! ATGTTTGAAAGTGTACCTAA+AGG - chr3.2:37339722-37339741 MS.gene91989:CDS 30.0%
! CTATATCGACTGAAACTTTA+GGG - chr3.2:37339619-37339638 MS.gene91989:CDS 30.0%
!! ATAAAAAGGCTTGTTGATGT+TGG - chr3.2:37339365-37339384 MS.gene91989:intron 30.0%
AAGAATACAATTCGAGAAGG+GGG - chr3.2:37339194-37339213 MS.gene91989:CDS 35.0%
GGTGTCAAATGATTAGATGT+TGG - chr3.2:37339593-37339612 MS.gene91989:CDS 35.0%
TCAAAACACCCTCATATACA+TGG - chr3.2:37339422-37339441 MS.gene91989:intron 35.0%
TCATGGCTGTATTGAAAACT+TGG + chr3.2:37339286-37339305 None:intergenic 35.0%
! GCTATATCGACTGAAACTTT+AGG - chr3.2:37339618-37339637 MS.gene91989:CDS 35.0%
AAGATCATACCAGGATACGA+AGG + chr3.2:37339485-37339504 None:intergenic 40.0%
AAGGTTCTTGAGAGCTACAA+TGG - chr3.2:37339332-37339351 MS.gene91989:intron 40.0%
AGATCATACCAGGATACGAA+GGG + chr3.2:37339484-37339503 None:intergenic 40.0%
ATTATGCAAGCTTGGACTCA+AGG - chr3.2:37339255-37339274 MS.gene91989:intron 40.0%
TGAGAGCTACAATGGTTTCA+AGG - chr3.2:37339340-37339359 MS.gene91989:intron 40.0%
TGCCATGAACCATGTTGAAT+GGG + chr3.2:37339223-37339242 None:intergenic 40.0%
TGCGTTTGATTATGCAAGCT+TGG - chr3.2:37339247-37339266 MS.gene91989:intron 40.0%
!! AAAAGGCTTGTTGATGTTGG+AGG - chr3.2:37339368-37339387 MS.gene91989:intron 40.0%
GATCATACCAGGATACGAAG+GGG + chr3.2:37339483-37339502 None:intergenic 45.0%
GTCCCATTCAACATGGTTCA+TGG - chr3.2:37339218-37339237 MS.gene91989:CDS 45.0%
GTGCCATGAACCATGTTGAA+TGG + chr3.2:37339224-37339243 None:intergenic 45.0%
TTGATGTTGGAGGTGGTCTT+GGG - chr3.2:37339378-37339397 MS.gene91989:intron 45.0%
TTGGTAGGAGTTGAACACGT+TGG - chr3.2:37339692-37339711 MS.gene91989:CDS 45.0%
GGGGCATGTTGAATGACATG+AGG + chr3.2:37339464-37339483 None:intergenic 50.0%
GTAGGAGTTGAACACGTTGG+CGG - chr3.2:37339695-37339714 MS.gene91989:CDS 50.0%
GTTGATGTTGGAGGTGGTCT+TGG - chr3.2:37339377-37339396 MS.gene91989:intron 50.0%
!! AGGCTTGTTGATGTTGGAGG+TGG - chr3.2:37339371-37339390 MS.gene91989:intron 50.0%
CAACATGCCCCTTCGTATCC+TGG - chr3.2:37339473-37339492 MS.gene91989:CDS 55.0%
!! AGGGGGTGTCCCATTCAACA+TGG - chr3.2:37339211-37339230 MS.gene91989:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 37339176 37339763 37339176 ID=MS.gene91989
chr3.2 mRNA 37339176 37339763 37339176 ID=MS.gene91989.t1;Parent=MS.gene91989
chr3.2 exon 37339445 37339763 37339445 ID=MS.gene91989.t1.exon1;Parent=MS.gene91989.t1
chr3.2 CDS 37339445 37339763 37339445 ID=cds.MS.gene91989.t1;Parent=MS.gene91989.t1
chr3.2 exon 37339176 37339240 37339176 ID=MS.gene91989.t1.exon2;Parent=MS.gene91989.t1
chr3.2 CDS 37339176 37339240 37339176 ID=cds.MS.gene91989.t1;Parent=MS.gene91989.t1
Gene Sequence

>MS.gene91989

ATGCTTAGGTCCGAGCTTAAGAATACAATTCGAGAAGGGGGTGTCCCATTCAACATGGTTCATGGCACACATGCGTTTGATTATGCAAGCTTGGACTCAAGGTTCAACCAAGTTTTCAATACAGCCATGATCAATTATACCAAAATAGTTATGAAGAAGGTTCTTGAGAGCTACAATGGTTTCAAGGACATAAAAAGGCTTGTTGATGTTGGAGGTGGTCTTGGGATCAACATTAACTTGATCACTTCAAAACACCCTCATATACATGGTATCAATTTCGACTTGCCTCATGTCATTCAACATGCCCCTTCGTATCCTGGTATGATCTTTAAAATGTTATTTATCATTCTCAAAGAAATATATGTTTTATCTAAAATTTAAATTCACTCAGAATACTTACATATAAATAAGTAATGTGGTGTCAAATGATTAGATGTTGGATGCTATATCGACTGAAACTTTAGGGTATGAGAGTTCTAACAAAAAAAAAATCGCTAATTGAAAAACTATTTAATGTTGGTAGGAGTTGAACACGTTGGCGGAGATATGTTTGAAAGTGTACCTAAAGGAGATGCAATCTTTATGAAG
Protein sequence

>MS.gene91989.t1

MLRSELKNTIREGGVPFNMVHGTHAFDYASLDSRFNQVFNTAMINYTKIVMKKVLESYNGFKDIKRLVDVGGGLGININLITSKHPHIHGINFDLPHVIQHAPSYPGVEHVGGDMFESVPKGDAIFMK