AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92019


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92019.t1 MTR_7g018500 95.467 375 14 3 1 373 1 374 0.0 552
MS.gene92019.t1 MTR_6g017055 48.596 356 102 16 73 366 67 403 2.15e-75 239
MS.gene92019.t1 MTR_1g103380 35.417 384 163 15 41 356 119 485 4.22e-43 156
MS.gene92019.t1 MTR_4g111935 33.459 266 141 11 5 245 14 268 2.49e-26 108
MS.gene92019.t1 MTR_2g078200 58.333 60 25 0 120 179 20 79 1.62e-16 79.7
MS.gene92019.t1 MTR_2g090960 51.562 64 31 0 115 178 19 82 4.47e-16 79.0
MS.gene92019.t1 MTR_6g015350 48.750 80 39 1 121 200 50 127 4.55e-16 79.0
MS.gene92019.t1 MTR_8g463380 52.308 65 31 0 115 179 49 113 5.04e-16 79.3
MS.gene92019.t1 MTR_4g079580 52.308 65 31 0 115 179 17 81 6.14e-16 78.6
MS.gene92019.t1 MTR_7g015010 53.448 58 27 0 121 178 58 115 2.31e-14 73.9
MS.gene92019.t1 MTR_4g109660 50.000 58 29 0 121 178 57 114 6.82e-13 68.9
MS.gene92019.t1 MTR_3g026050 50.000 58 29 0 121 178 58 115 3.04e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92019.t1 AT1G67260 37.687 268 130 11 73 312 47 305 6.57e-35 131
MS.gene92019.t1 AT1G67260 37.687 268 130 11 73 312 47 305 6.57e-35 131
MS.gene92019.t1 AT1G67260 37.687 268 130 11 73 312 47 305 6.71e-35 132
MS.gene92019.t1 AT1G68800 54.867 113 42 4 120 229 112 218 1.66e-25 106
MS.gene92019.t1 AT1G68800 54.867 113 42 4 120 229 112 218 1.98e-25 105
MS.gene92019.t1 AT3G18550 38.562 153 70 3 113 241 141 293 5.76e-21 94.0
MS.gene92019.t1 AT3G18550 36.306 157 72 3 113 241 141 297 9.12e-20 90.5
MS.gene92019.t1 AT3G18550 36.306 157 72 3 113 241 141 297 1.02e-19 90.5
MS.gene92019.t1 AT3G18550 36.306 157 72 3 113 241 141 297 1.02e-19 90.5
MS.gene92019.t1 AT4G18390 36.986 146 88 3 103 248 27 168 2.73e-17 82.8
MS.gene92019.t1 AT4G18390 36.986 146 88 3 103 248 27 168 2.73e-17 82.8
MS.gene92019.t1 AT1G30210 58.621 58 24 0 121 178 51 108 1.81e-16 79.7
MS.gene92019.t1 AT1G30210 58.621 58 24 0 121 178 51 108 1.81e-16 79.7
MS.gene92019.t1 AT1G53230 51.515 66 32 0 115 180 44 109 5.29e-16 79.3
MS.gene92019.t1 AT1G53230 51.515 66 32 0 115 180 44 109 5.29e-16 79.3
MS.gene92019.t1 AT3G15030 52.308 65 31 0 115 179 40 104 6.59e-16 79.0
MS.gene92019.t1 AT3G15030 52.308 65 31 0 115 179 40 104 6.59e-16 79.0
MS.gene92019.t1 AT3G15030 52.308 65 31 0 115 179 40 104 6.59e-16 79.0
MS.gene92019.t1 AT3G15030 52.308 65 31 0 115 179 40 104 6.59e-16 79.0
MS.gene92019.t1 AT2G31070 51.562 64 31 0 115 178 24 87 1.66e-15 77.4
MS.gene92019.t1 AT3G02150 55.172 58 26 0 121 178 75 132 1.08e-14 73.9
MS.gene92019.t1 AT3G02150 55.172 58 26 0 121 178 75 132 1.85e-14 74.3
MS.gene92019.t1 AT5G60970 48.276 58 30 0 121 178 64 121 1.19e-12 68.9
MS.gene92019.t1 AT5G08070 46.552 58 31 0 121 178 34 91 2.82e-11 63.5
MS.gene92019.t1 AT5G08070 46.552 58 31 0 121 178 34 91 2.82e-11 63.5

Find 61 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCAACTTATTGCTCTTT+AGG 0.129653 7.3:-79736493 MS.gene92019:CDS
ATCCTGATACACTAACAAAT+TGG 0.193845 7.3:-79736267 MS.gene92019:CDS
GCCCAATTTGTTAGTGTATC+AGG 0.208804 7.3:+79736265 None:intergenic
CTCCAATGTTATCACCAAAT+TGG 0.217754 7.3:-79735538 MS.gene92019:CDS
GTTAGTGTATCAGGATTAAT+TGG 0.223236 7.3:+79736274 None:intergenic
CTTCGAAGACGAAGGATTCT+AGG 0.286350 7.3:-79735832 MS.gene92019:CDS
CTATGCCTGTCTTTCTTAAC+CGG 0.314288 7.3:+79736148 None:intergenic
TTCGATCTTCAAGACATGTT+AGG 0.334610 7.3:-79736055 MS.gene92019:CDS
TTTGAAGTTTCATCCTCATT+AGG 0.336904 7.3:+79735695 None:intergenic
TTATTATTAGCATCCCAATT+TGG 0.341400 7.3:+79735524 None:intergenic
TCATGAGAATCACTATTATT+AGG 0.353829 7.3:+79735563 None:intergenic
TCAGGGGTAAGGAAAGTAAA+AGG 0.368203 7.3:+79736418 None:intergenic
AATATCAGCTAATATCGATT+TGG 0.375751 7.3:+79735255 None:intergenic
TTCTTAACCGGTTTCTTGAC+CGG 0.388342 7.3:+79736160 None:intergenic
GAGCCATTCAAGTGTGTTGC+TGG 0.409129 7.3:+79736021 None:intergenic
TTCAAATCTTTGGAAAATCA+TGG 0.409744 7.3:-79735296 MS.gene92019:CDS
TCCAATGTTATCACCAAATT+GGG 0.411724 7.3:-79735537 MS.gene92019:CDS
CTAAAGATAGAAAGATGAAA+AGG 0.414926 7.3:-79735868 MS.gene92019:CDS
TCTTGATATTGCAGAAAAGT+TGG 0.424180 7.3:+79735475 None:intergenic
TCCTGATACACTAACAAATT+GGG 0.434497 7.3:-79736266 MS.gene92019:CDS
TCGATCTTCAAGACATGTTA+GGG 0.442491 7.3:-79736054 MS.gene92019:CDS
AGCAAGAACAACATAGCTTC+TGG 0.450038 7.3:-79735959 MS.gene92019:CDS
TTCATCTCCTTCATCGGACG+AGG 0.483567 7.3:+79735901 None:intergenic
TCTTCTTCCTCGTCCGATGA+AGG 0.488840 7.3:-79735908 MS.gene92019:CDS
TGATGAGTTGTTTGTGTGTA+GGG 0.490224 7.3:+79736304 None:intergenic
AATAACAATGATGCTGTCAC+TGG 0.496363 7.3:-79735503 MS.gene92019:CDS
AAAGAAACCGGTCAAGAAAC+CGG 0.496709 7.3:-79736167 MS.gene92019:CDS
AAATTTGCTCACAAAGAAAC+CGG 0.498565 7.3:-79736179 MS.gene92019:CDS
CATTAGGCATCAATTGATGA+AGG 0.499616 7.3:+79735711 None:intergenic
ATCTTCTTCATCTCCTTCAT+CGG 0.500031 7.3:+79735895 None:intergenic
CCTAAAGAGCAATAAGTTGA+AGG 0.500471 7.3:+79736493 None:intergenic
AGCAAGATTCACACATCTCA+AGG 0.503482 7.3:-79736127 MS.gene92019:CDS
TCAAGAACGAATCTATCATC+AGG 0.510472 7.3:-79735458 MS.gene92019:intron
TGTGATGAAGAAGATGAGTT+AGG 0.521777 7.3:+79736451 None:intergenic
TAAAGATAGAAAGATGAAAA+GGG 0.526209 7.3:-79735867 MS.gene92019:CDS
TCAAATCTTTGGAAAATCAT+GGG 0.526493 7.3:-79735295 MS.gene92019:CDS
GAACTTCCTCGCGATCTCGA+TGG 0.528472 7.3:+79736078 None:intergenic
AAGCCAGCAACACACTTGAA+TGG 0.532128 7.3:-79736024 MS.gene92019:CDS
TCATCAATTGATGCCTAATG+AGG 0.536962 7.3:-79735708 MS.gene92019:CDS
TTCGAAGACGAAGGATTCTA+GGG 0.537014 7.3:-79735831 MS.gene92019:CDS
AGAAAGCAAGAGCAAGAACA+AGG 0.538860 7.3:-79735808 MS.gene92019:CDS
TGATATCTTTAACTTCATTG+AGG 0.548363 7.3:-79735657 MS.gene92019:CDS
AGAAACCGGTTAAGAAAGAC+AGG 0.551030 7.3:-79736153 MS.gene92019:CDS
TAGGGAACAGAAATTGCTGC+TGG 0.557496 7.3:+79736322 None:intergenic
TCCCAATTTGGTGATAACAT+TGG 0.563066 7.3:+79735536 None:intergenic
TTAGGATTATAACAAGGGTA+AGG 0.565101 7.3:+79736469 None:intergenic
GATGAGTTAGGATTATAACA+AGG 0.570713 7.3:+79736463 None:intergenic
ATGATGAGTTGTTTGTGTGT+AGG 0.581066 7.3:+79736303 None:intergenic
GGGTAAGGAAAGTAAAAGGA+GGG 0.582814 7.3:+79736422 None:intergenic
CAGCTAATATCGATTTGGAC+TGG 0.588709 7.3:+79735260 None:intergenic
GGGGTAAGGAAAGTAAAAGG+AGG 0.592199 7.3:+79736421 None:intergenic
ATGAGTTAGGATTATAACAA+GGG 0.601724 7.3:+79736464 None:intergenic
GACTTTCCATCGAGATCGCG+AGG 0.608900 7.3:-79736084 MS.gene92019:CDS
CTCAAGGGTTGAGAGACAGA+AGG 0.613193 7.3:-79736111 MS.gene92019:CDS
GAAAGCAAGAGCAAGAACAA+GGG 0.614345 7.3:-79735807 MS.gene92019:CDS
GCTTCTGGCGATGACGATGA+TGG 0.616062 7.3:-79735944 MS.gene92019:CDS
GAAAGAATCTTCGAAGACGA+AGG 0.618129 7.3:-79735840 MS.gene92019:CDS
TCAAGGGTTGAGAGACAGAA+GGG 0.623957 7.3:-79736110 MS.gene92019:CDS
AATCTTTGGAAAATCATGGG+AGG 0.624471 7.3:-79735292 MS.gene92019:CDS
GCAAGATTCACACATCTCAA+GGG 0.635283 7.3:-79736126 MS.gene92019:CDS
ATGTTGAAACAAGATCTAAG+TGG 0.698091 7.3:-79736223 MS.gene92019:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAATTAATTAACAAAA+AGG - chr7.3:79736369-79736388 MS.gene92019:CDS 10.0%
!!! TTTTAGGTTTAAACTTTTTA+GGG - chr7.3:79736442-79736461 MS.gene92019:CDS 15.0%
!!! TTTTTAGGTTTAAACTTTTT+AGG - chr7.3:79736441-79736460 MS.gene92019:CDS 15.0%
!! TAAAGATAGAAAGATGAAAA+GGG - chr7.3:79735895-79735914 MS.gene92019:CDS 20.0%
!!! TTGTGATACTTACAATTTTT+AGG - chr7.3:79736426-79736445 MS.gene92019:CDS 20.0%
! ATGAGTTAGGATTATAACAA+GGG + chr7.3:79735301-79735320 None:intergenic 25.0%
! CTAAAGATAGAAAGATGAAA+AGG - chr7.3:79735894-79735913 MS.gene92019:CDS 25.0%
! TCATGAGAATCACTATTATT+AGG + chr7.3:79736202-79736221 None:intergenic 25.0%
! TTATTATTAGCATCCCAATT+TGG + chr7.3:79736241-79736260 None:intergenic 25.0%
!! TCAAATCTTTGGAAAATCAT+GGG - chr7.3:79736467-79736486 MS.gene92019:CDS 25.0%
!! TGATATCTTTAACTTCATTG+AGG - chr7.3:79736105-79736124 MS.gene92019:CDS 25.0%
!! TTCAAATCTTTGGAAAATCA+TGG - chr7.3:79736466-79736485 MS.gene92019:CDS 25.0%
!!! TGTTGATTTTGATCAATTTC+AGG + chr7.3:79736028-79736047 None:intergenic 25.0%
ATCCTGATACACTAACAAAT+TGG - chr7.3:79735495-79735514 MS.gene92019:CDS 30.0%
ATGTTGAAACAAGATCTAAG+TGG - chr7.3:79735539-79735558 MS.gene92019:CDS 30.0%
GATGAGTTAGGATTATAACA+AGG + chr7.3:79735302-79735321 None:intergenic 30.0%
GTTAGTGTATCAGGATTAAT+TGG + chr7.3:79735491-79735510 None:intergenic 30.0%
TCCAATGTTATCACCAAATT+GGG - chr7.3:79736225-79736244 MS.gene92019:CDS 30.0%
TCCTGATACACTAACAAATT+GGG - chr7.3:79735496-79735515 MS.gene92019:CDS 30.0%
TCTTGATATTGCAGAAAAGT+TGG + chr7.3:79736290-79736309 None:intergenic 30.0%
TTAGGATTATAACAAGGGTA+AGG + chr7.3:79735296-79735315 None:intergenic 30.0%
! AAATTTGCTCACAAAGAAAC+CGG - chr7.3:79735583-79735602 MS.gene92019:CDS 30.0%
! TTTGAAGTTTCATCCTCATT+AGG + chr7.3:79736070-79736089 None:intergenic 30.0%
!!! TAGAAGAAGCATTTTGATCA+GGG + chr7.3:79735364-79735383 None:intergenic 30.0%
!!! TTAGAAGAAGCATTTTGATC+AGG + chr7.3:79735365-79735384 None:intergenic 30.0%
!!! TTTTTAGGGCTTCAAATCTT+TGG - chr7.3:79736456-79736475 MS.gene92019:CDS 30.0%
AATAACAATGATGCTGTCAC+TGG - chr7.3:79736259-79736278 MS.gene92019:CDS 35.0%
ATCTTCTTCATCTCCTTCAT+CGG + chr7.3:79735870-79735889 None:intergenic 35.0%
ATGATGAGTTGTTTGTGTGT+AGG + chr7.3:79735462-79735481 None:intergenic 35.0%
CATTAGGCATCAATTGATGA+AGG + chr7.3:79736054-79736073 None:intergenic 35.0%
CCTTCAACTTATTGCTCTTT+AGG - chr7.3:79735269-79735288 MS.gene92019:CDS 35.0%
CTCCAATGTTATCACCAAAT+TGG - chr7.3:79736224-79736243 MS.gene92019:CDS 35.0%
TCAAGAACGAATCTATCATC+AGG - chr7.3:79736304-79736323 MS.gene92019:CDS 35.0%
TCATCAATTGATGCCTAATG+AGG - chr7.3:79736054-79736073 MS.gene92019:CDS 35.0%
TCGATCTTCAAGACATGTTA+GGG - chr7.3:79735708-79735727 MS.gene92019:CDS 35.0%
TGATGAGTTGTTTGTGTGTA+GGG + chr7.3:79735461-79735480 None:intergenic 35.0%
TGTGATGAAGAAGATGAGTT+AGG + chr7.3:79735314-79735333 None:intergenic 35.0%
TTCGATCTTCAAGACATGTT+AGG - chr7.3:79735707-79735726 MS.gene92019:CDS 35.0%
! AATCTTTGGAAAATCATGGG+AGG - chr7.3:79736470-79736489 MS.gene92019:CDS 35.0%
! CCTAAAGAGCAATAAGTTGA+AGG + chr7.3:79735272-79735291 None:intergenic 35.0%
! TCCCAATTTGGTGATAACAT+TGG + chr7.3:79736229-79736248 None:intergenic 35.0%
!!! AGAAGAAGCATTTTGATCAG+GGG + chr7.3:79735363-79735382 None:intergenic 35.0%
AAAGAAACCGGTCAAGAAAC+CGG - chr7.3:79735595-79735614 MS.gene92019:CDS 40.0%
AGAAACCGGTTAAGAAAGAC+AGG - chr7.3:79735609-79735628 MS.gene92019:CDS 40.0%
AGAAAGCAAGAGCAAGAACA+AGG - chr7.3:79735954-79735973 MS.gene92019:CDS 40.0%
AGCAAGAACAACATAGCTTC+TGG - chr7.3:79735803-79735822 MS.gene92019:CDS 40.0%
AGCAAGATTCACACATCTCA+AGG - chr7.3:79735635-79735654 MS.gene92019:CDS 40.0%
CTATGCCTGTCTTTCTTAAC+CGG + chr7.3:79735617-79735636 None:intergenic 40.0%
GAAAGAATCTTCGAAGACGA+AGG - chr7.3:79735922-79735941 MS.gene92019:CDS 40.0%
GAAAGCAAGAGCAAGAACAA+GGG - chr7.3:79735955-79735974 MS.gene92019:CDS 40.0%
GCAAGATTCACACATCTCAA+GGG - chr7.3:79735636-79735655 MS.gene92019:CDS 40.0%
GGGTAAGGAAAGTAAAAGGA+GGG + chr7.3:79735343-79735362 None:intergenic 40.0%
TCAGGGGTAAGGAAAGTAAA+AGG + chr7.3:79735347-79735366 None:intergenic 40.0%
TTCGAAGACGAAGGATTCTA+GGG - chr7.3:79735931-79735950 MS.gene92019:CDS 40.0%
! TTCTTAACCGGTTTCTTGAC+CGG + chr7.3:79735605-79735624 None:intergenic 40.0%
!! AAGCATTTTGATCAGGGGTA+AGG + chr7.3:79735358-79735377 None:intergenic 40.0%
!! GCCCAATTTGTTAGTGTATC+AGG + chr7.3:79735500-79735519 None:intergenic 40.0%
AAGCCAGCAACACACTTGAA+TGG - chr7.3:79735738-79735757 MS.gene92019:CDS 45.0%
CTTCGAAGACGAAGGATTCT+AGG - chr7.3:79735930-79735949 MS.gene92019:CDS 45.0%
GGGGTAAGGAAAGTAAAAGG+AGG + chr7.3:79735344-79735363 None:intergenic 45.0%
TAGGGAACAGAAATTGCTGC+TGG + chr7.3:79735443-79735462 None:intergenic 45.0%
TCAAGGGTTGAGAGACAGAA+GGG - chr7.3:79735652-79735671 MS.gene92019:CDS 45.0%
CTCAAGGGTTGAGAGACAGA+AGG - chr7.3:79735651-79735670 MS.gene92019:CDS 50.0%
GAGCCATTCAAGTGTGTTGC+TGG + chr7.3:79735744-79735763 None:intergenic 50.0%
TCTTCTTCCTCGTCCGATGA+AGG - chr7.3:79735854-79735873 MS.gene92019:CDS 50.0%
TTCATCTCCTTCATCGGACG+AGG + chr7.3:79735864-79735883 None:intergenic 50.0%
GAACTTCCTCGCGATCTCGA+TGG + chr7.3:79735687-79735706 None:intergenic 55.0%
GACTTTCCATCGAGATCGCG+AGG - chr7.3:79735678-79735697 MS.gene92019:CDS 55.0%
GCTTCTGGCGATGACGATGA+TGG - chr7.3:79735818-79735837 MS.gene92019:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 79735263 79736521 79735263 ID=MS.gene92019
chr7.3 mRNA 79735263 79736521 79735263 ID=MS.gene92019.t1;Parent=MS.gene92019
chr7.3 exon 79735459 79736521 79735459 ID=MS.gene92019.t1.exon1;Parent=MS.gene92019.t1
chr7.3 CDS 79735459 79736521 79735459 ID=cds.MS.gene92019.t1;Parent=MS.gene92019.t1
chr7.3 exon 79735263 79735321 79735263 ID=MS.gene92019.t1.exon2;Parent=MS.gene92019.t1
chr7.3 CDS 79735263 79735321 79735263 ID=cds.MS.gene92019.t1;Parent=MS.gene92019.t1
Gene Sequence

>MS.gene92019

ATGTTTCCTTCAACTTATTGCTCTTTAGGTCCTTACCCTTGTTATAATCCTAACTCATCTTCTTCATCACATTCATACCCTCCTTTTACTTTCCTTACCCCTGATCAAAATGCTTCTTCTAACAACAACAACAATAACAACAACAGCAACAACAACATCAACAACTTTCTTCATGATCCAGCAGCAATTTCTGTTCCCTACACACAAACAACTCATCATCATGTTCCAATTAATCCTGATACACTAACAAATTGGGCTGTTGCTGACTATGCAGCTATGTTGAAACAAGATCTAAGTGGTTCTTCTCATTACAATCTCTCAAATTTGCTCACAAAGAAACCGGTCAAGAAACCGGTTAAGAAAGACAGGCATAGCAAGATTCACACATCTCAAGGGTTGAGAGACAGAAGGGTGAGACTTTCCATCGAGATCGCGAGGAAGTTCTTCGATCTTCAAGACATGTTAGGGTTTGACAAAGCCAGCAACACACTTGAATGGCTCTTCAATAAATCAAAAGAAGCAATTGAAGAGTTAACTAGAAGCAAGAACAACATAGCTTCTGGCGATGACGATGATGGTGATCATAGCTTCTCTTCTTCCTCGTCCGATGAAGGAGATGAAGAAGATTATACTAAAGATAGAAAGATGAAAAGGGCACAGAAAGAATCTTCGAAGACGAAGGATTCTAGGGAGAAAGCAAGAGCAAGAACAAGGGAAAGAGCTAGTGAGATGAAGATGCAAGAAGATTTGAAAGAAAAATATCCTGAAATTGATCAAAATCAACAAATCCTTCATCAATTGATGCCTAATGAGGATGAAACTTCAAAATCACTTCAAAGAGATGATATCTTTAACTTCATTGAGGAATCTATTGTGATTAAGAGAAAGTTGAAGAAATCTTCTTCTCATCATTATCAACAAAACACTAATTTGATGCCTAATAATAGTGATTCTCATGACTCTCCAATGTTATCACCAAATTGGGATGCTAATAATAATAACAATGATGCTGTCACTGGAAGATCCAACTTTTCTGCAATATCAAGAACGAATCTATCATCAGGTAATTTTTGTTTTTATTTGATAATTGTTTCACTGTTTCAAAACATAAAATTAATTAACAAAAAGGCTAATTTTTTATTTTATTTTTATTTTTGTGAATTTTGTGATACTTACAATTTTTAGGTTTAAACTTTTTAGGGCTTCAAATCTTTGGAAAATCATGGGAGGATTGCAATACCAGTCCAAATCGATATTAG

Protein sequence

>MS.gene92019.t1

MFPSTYCSLGPYPCYNPNSSSSSHSYPPFTFLTPDQNASSNNNNNNNNSNNNINNFLHDPAAISVPYTQTTHHHVPINPDTLTNWAVADYAAMLKQDLSGSSHYNLSNLLTKKPVKKPVKKDRHSKIHTSQGLRDRRVRLSIEIARKFFDLQDMLGFDKASNTLEWLFNKSKEAIEELTRSKNNIASGDDDDGDHSFSSSSSDEGDEEDYTKDRKMKRAQKESSKTKDSREKARARTRERASEMKMQEDLKEKYPEIDQNQQILHQLMPNEDETSKSLQRDDIFNFIEESIVIKRKLKKSSSHHYQQNTNLMPNNSDSHDSPMLSPNWDANNNNNDAVTGRSNFSAISRTNLSSGLQIFGKSWEDCNTSPNRY