Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92225.t1 | RHN44081.1 | 88.2 | 93 | 11 | 0 | 1 | 93 | 5 | 97 | 5.60E-34 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92225.t1 | A0A072TWH2 | 88.2 | 93 | 11 | 0 | 1 | 93 | 18 | 110 | 4.0e-34 | 153.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057693 | MS.gene92225 | 0.818342 | 2.06E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92225.t1 | MTR_7g007290 | 88.172 | 93 | 11 | 0 | 1 | 93 | 18 | 110 | 3.41e-55 | 166 |
| MS.gene92225.t1 | MTR_7g007300 | 77.143 | 70 | 16 | 0 | 1 | 70 | 1 | 70 | 7.09e-30 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92225.t1 | AT1G47820 | 55.714 | 70 | 30 | 1 | 1 | 69 | 1 | 70 | 2.68e-20 | 78.2 |
| MS.gene92225.t1 | AT1G47820 | 55.714 | 70 | 30 | 1 | 1 | 69 | 1 | 70 | 2.68e-20 | 78.2 |
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCATTCACCTGAGGTCT+AGG | 0.432602 | 7.1:-87085103 | None:intergenic |
| ACAAAGCATGGCCGTATTGA+AGG | 0.443308 | 7.1:+87085207 | MS.gene92225:CDS |
| CACAGAGTCATATTATGTTC+AGG | 0.457008 | 7.1:+87085138 | MS.gene92225:CDS |
| TATTATGTTCAGGCAATCAA+TGG | 0.461084 | 7.1:+87085148 | MS.gene92225:CDS |
| TGTTATGAAATCCTTCAATA+CGG | 0.467267 | 7.1:-87085218 | None:intergenic |
| TGACGATGATGTTGCTAAGA+AGG | 0.498240 | 7.1:+87085297 | MS.gene92225:CDS |
| TGCAGTGCTTCCTAGACCTC+AGG | 0.506425 | 7.1:+87085093 | MS.gene92225:CDS |
| TGCTAAGAAGGCTCAAGTAA+AGG | 0.526582 | 7.1:+87085309 | MS.gene92225:CDS |
| GAAGCTTCTAACGACTCGTC+TGG | 0.528210 | 7.1:-87085265 | None:intergenic |
| CGAGTCGTTAGAAGCTTCCT+CGG | 0.555746 | 7.1:+87085270 | MS.gene92225:CDS |
| GACTCGTCTGGAACATCTTG+AGG | 0.569302 | 7.1:-87085253 | None:intergenic |
| TAACATGAAAGAACAAAGCA+TGG | 0.616394 | 7.1:+87085195 | MS.gene92225:CDS |
| AACATCATCGTCATCTTCCG+AGG | 0.626307 | 7.1:-87085287 | None:intergenic |
| CGAAATGTGGCATTCACCTG+AGG | 0.643629 | 7.1:-87085109 | None:intergenic |
| CTCTGTGCTGAGACGAAATG+TGG | 0.644541 | 7.1:-87085122 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTTATGAAATCCTTCAATA+CGG | - | chr7.1:87085221-87085240 | None:intergenic | 25.0% |
| TAACATGAAAGAACAAAGCA+TGG | + | chr7.1:87085195-87085214 | MS.gene92225:CDS | 30.0% | |
| TATTATGTTCAGGCAATCAA+TGG | + | chr7.1:87085148-87085167 | MS.gene92225:CDS | 30.0% | |
| CACAGAGTCATATTATGTTC+AGG | + | chr7.1:87085138-87085157 | MS.gene92225:CDS | 35.0% | |
| TGACGATGATGTTGCTAAGA+AGG | + | chr7.1:87085297-87085316 | MS.gene92225:CDS | 40.0% | |
| TGCTAAGAAGGCTCAAGTAA+AGG | + | chr7.1:87085309-87085328 | MS.gene92225:CDS | 40.0% | |
| AACATCATCGTCATCTTCCG+AGG | - | chr7.1:87085290-87085309 | None:intergenic | 45.0% | |
| ACAAAGCATGGCCGTATTGA+AGG | + | chr7.1:87085207-87085226 | MS.gene92225:CDS | 45.0% | |
| CGAAATGTGGCATTCACCTG+AGG | - | chr7.1:87085112-87085131 | None:intergenic | 50.0% | |
| CGAGTCGTTAGAAGCTTCCT+CGG | + | chr7.1:87085270-87085289 | MS.gene92225:CDS | 50.0% | |
| CTCTGTGCTGAGACGAAATG+TGG | - | chr7.1:87085125-87085144 | None:intergenic | 50.0% | |
| GAAGCTTCTAACGACTCGTC+TGG | - | chr7.1:87085268-87085287 | None:intergenic | 50.0% | |
| ! | GACTCGTCTGGAACATCTTG+AGG | - | chr7.1:87085256-87085275 | None:intergenic | 50.0% |
| GTGGCATTCACCTGAGGTCT+AGG | - | chr7.1:87085106-87085125 | None:intergenic | 55.0% | |
| TGCAGTGCTTCCTAGACCTC+AGG | + | chr7.1:87085093-87085112 | MS.gene92225:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 87085073 | 87085354 | 87085073 | ID=MS.gene92225 |
| chr7.1 | mRNA | 87085073 | 87085354 | 87085073 | ID=MS.gene92225.t1;Parent=MS.gene92225 |
| chr7.1 | exon | 87085073 | 87085354 | 87085073 | ID=MS.gene92225.t1.exon1;Parent=MS.gene92225.t1 |
| chr7.1 | CDS | 87085073 | 87085354 | 87085073 | ID=cds.MS.gene92225.t1;Parent=MS.gene92225.t1 |
| Gene Sequence |
| Protein sequence |
