Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92284.t1 | XP_003610732.2 | 93 | 172 | 10 | 2 | 1 | 171 | 1 | 171 | 6.30E-84 | 320.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92284.t1 | G7K3N7 | 93.0 | 172 | 10 | 2 | 1 | 171 | 1 | 171 | 4.5e-84 | 320.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060495 | MS.gene92284 | 0.822955 | 1.80E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92284.t1 | MTR_5g006400 | 93.023 | 172 | 10 | 2 | 1 | 171 | 1 | 171 | 2.53e-115 | 324 |
| MS.gene92284.t1 | MTR_1g103570 | 64.085 | 142 | 43 | 3 | 10 | 149 | 8 | 143 | 2.41e-57 | 177 |
| MS.gene92284.t1 | MTR_1g071920 | 42.553 | 141 | 68 | 3 | 21 | 148 | 9 | 149 | 2.99e-27 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92284.t1 | AT3G22530 | 59.322 | 118 | 43 | 2 | 41 | 153 | 59 | 176 | 2.81e-40 | 135 |
| MS.gene92284.t1 | AT4G14830 | 63.793 | 116 | 38 | 3 | 41 | 152 | 28 | 143 | 1.11e-39 | 132 |
Find 41 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTGGAGGTTTCGTTTGC+CGG | 0.284682 | 5.3:-1487145 | MS.gene92284:CDS |
| TCTTAGTTTGTTGTTGTTGT+TGG | 0.335493 | 5.3:+1487389 | None:intergenic |
| ATGATCTTCATCCTGATATC+TGG | 0.364083 | 5.3:-1487161 | MS.gene92284:CDS |
| AAACGAAACCTCCAGATATC+AGG | 0.375554 | 5.3:+1487150 | None:intergenic |
| ACCTACCTGTTTCCGATTTG+TGG | 0.379340 | 5.3:-1487292 | MS.gene92284:CDS |
| GAAACACTCTTGTCTGTGTC+TGG | 0.410833 | 5.3:-1487417 | MS.gene92284:CDS |
| TTTGCCGGAATCTGTTGTTC+CGG | 0.426638 | 5.3:-1487130 | MS.gene92284:CDS |
| CTCCGCCACAAATCGGAAAC+AGG | 0.434356 | 5.3:+1487287 | None:intergenic |
| AGCTCCGGAACAACAGATTC+CGG | 0.463331 | 5.3:+1487126 | None:intergenic |
| GAGACCATTCCTGAACCTAT+TGG | 0.472661 | 5.3:-1487042 | MS.gene92284:CDS |
| GTCTCCCTCACGTGTTCAGC+AGG | 0.474728 | 5.3:-1487365 | MS.gene92284:CDS |
| GTGTCACTAAGATTGTTGTA+AGG | 0.490134 | 5.3:-1487212 | MS.gene92284:CDS |
| GAACCTATTGGTGGTGGTGG+TGG | 0.495438 | 5.3:-1487030 | MS.gene92284:CDS |
| CCGACCGTAGCGGAAGTTGC+AGG | 0.500550 | 5.3:+1487340 | None:intergenic |
| CGTCTGTCTCCGCCACAAAT+CGG | 0.500659 | 5.3:+1487280 | None:intergenic |
| CCACCACCACCAATAGGTTC+AGG | 0.502881 | 5.3:+1487033 | None:intergenic |
| ACTCATCGTCACCGTGCCTA+AGG | 0.516775 | 5.3:-1487076 | MS.gene92284:CDS |
| CCTGAACCTATTGGTGGTGG+TGG | 0.519897 | 5.3:-1487033 | MS.gene92284:CDS |
| AACACAGCAGTAGCGAGCTC+CGG | 0.534860 | 5.3:+1487111 | None:intergenic |
| AGTGTTTCTGCTTGAGACAG+TGG | 0.538516 | 5.3:+1487432 | None:intergenic |
| GCTACTGCTGTGTTTGTCGA+TGG | 0.552059 | 5.3:-1487102 | MS.gene92284:CDS |
| CACACGCTTCATATCCATCC+AGG | 0.559660 | 5.3:-1487234 | MS.gene92284:CDS |
| TGTCACTAAGATTGTTGTAA+GGG | 0.575511 | 5.3:-1487211 | MS.gene92284:CDS |
| ATCTTCATCCTGATATCTGG+AGG | 0.587374 | 5.3:-1487158 | MS.gene92284:CDS |
| CAATCTTAGTGACACCTGGA+TGG | 0.590009 | 5.3:+1487220 | None:intergenic |
| GGATGCTGACGTGTCAGTTG+AGG | 0.599188 | 5.3:-1487319 | MS.gene92284:CDS |
| ATTCCTGAACCTATTGGTGG+TGG | 0.599552 | 5.3:-1487036 | MS.gene92284:CDS |
| CCTGCAACTTCCGCTACGGT+CGG | 0.606468 | 5.3:-1487340 | MS.gene92284:CDS |
| AGACGCATCACTCGGACATG+TGG | 0.606866 | 5.3:-1487262 | MS.gene92284:CDS |
| ACAACAATCTTAGTGACACC+TGG | 0.610797 | 5.3:+1487216 | None:intergenic |
| GTCTCATCGTCACAAGCCTT+AGG | 0.613900 | 5.3:+1487060 | None:intergenic |
| ACCATTCCTGAACCTATTGG+TGG | 0.619569 | 5.3:-1487039 | MS.gene92284:CDS |
| AGGATCCTGCTGAACACGTG+AGG | 0.619719 | 5.3:+1487360 | None:intergenic |
| TACCTGTTTCCGATTTGTGG+CGG | 0.624557 | 5.3:-1487289 | MS.gene92284:CDS |
| GGATCCTGCTGAACACGTGA+GGG | 0.626553 | 5.3:+1487361 | None:intergenic |
| GGATCCTGCAACTTCCGCTA+CGG | 0.628844 | 5.3:-1487344 | MS.gene92284:CDS |
| GCGGAGACAGACGCATCACT+CGG | 0.632529 | 5.3:-1487270 | MS.gene92284:CDS |
| ACCACCAATAGGTTCAGGAA+TGG | 0.647164 | 5.3:+1487038 | None:intergenic |
| ATCGTCACAAGCCTTAGGCA+CGG | 0.656268 | 5.3:+1487065 | None:intergenic |
| GCCACAAATCGGAAACAGGT+AGG | 0.667827 | 5.3:+1487291 | None:intergenic |
| ACGTCAGCATCCGACCGTAG+CGG | 0.709541 | 5.3:+1487330 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGCAATGAGTTGCAAAAAA+TGG | - | chr5.3:1487018-1487037 | MS.gene92284:CDS | 30.0% | |
| TGTCACTAAGATTGTTGTAA+GGG | - | chr5.3:1487292-1487311 | MS.gene92284:CDS | 30.0% | |
| !! | TCTTAGTTTGTTGTTGTTGT+TGG | + | chr5.3:1487117-1487136 | None:intergenic | 30.0% |
| ATGATCTTCATCCTGATATC+TGG | - | chr5.3:1487342-1487361 | MS.gene92284:CDS | 35.0% | |
| ! | GTGTCACTAAGATTGTTGTA+AGG | - | chr5.3:1487291-1487310 | MS.gene92284:CDS | 35.0% |
| !! | TCACTCACTTCACTTTTCTT+TGG | - | chr5.3:1487319-1487338 | MS.gene92284:CDS | 35.0% |
| AAACGAAACCTCCAGATATC+AGG | + | chr5.3:1487356-1487375 | None:intergenic | 40.0% | |
| ACAACAATCTTAGTGACACC+TGG | + | chr5.3:1487290-1487309 | None:intergenic | 40.0% | |
| ATCTTCATCCTGATATCTGG+AGG | - | chr5.3:1487345-1487364 | MS.gene92284:CDS | 40.0% | |
| ACCACCAATAGGTTCAGGAA+TGG | + | chr5.3:1487468-1487487 | None:intergenic | 45.0% | |
| ACCATTCCTGAACCTATTGG+TGG | - | chr5.3:1487464-1487483 | MS.gene92284:CDS | 45.0% | |
| ATTCCTGAACCTATTGGTGG+TGG | - | chr5.3:1487467-1487486 | MS.gene92284:CDS | 45.0% | |
| CAATCTTAGTGACACCTGGA+TGG | + | chr5.3:1487286-1487305 | None:intergenic | 45.0% | |
| GAAACACTCTTGTCTGTGTC+TGG | - | chr5.3:1487086-1487105 | MS.gene92284:CDS | 45.0% | |
| GAGACCATTCCTGAACCTAT+TGG | - | chr5.3:1487461-1487480 | MS.gene92284:CDS | 45.0% | |
| TATCTGGAGGTTTCGTTTGC+CGG | - | chr5.3:1487358-1487377 | MS.gene92284:CDS | 45.0% | |
| TTTGCCGGAATCTGTTGTTC+CGG | - | chr5.3:1487373-1487392 | MS.gene92284:CDS | 45.0% | |
| ! | ACCTACCTGTTTCCGATTTG+TGG | - | chr5.3:1487211-1487230 | MS.gene92284:CDS | 45.0% |
| ! | AGTGTTTCTGCTTGAGACAG+TGG | + | chr5.3:1487074-1487093 | None:intergenic | 45.0% |
| ! | TACCTGTTTCCGATTTGTGG+CGG | - | chr5.3:1487214-1487233 | MS.gene92284:CDS | 45.0% |
| AGCTCCGGAACAACAGATTC+CGG | + | chr5.3:1487380-1487399 | None:intergenic | 50.0% | |
| ATCGTCACAAGCCTTAGGCA+CGG | + | chr5.3:1487441-1487460 | None:intergenic | 50.0% | |
| CACACGCTTCATATCCATCC+AGG | - | chr5.3:1487269-1487288 | MS.gene92284:CDS | 50.0% | |
| GCCACAAATCGGAAACAGGT+AGG | + | chr5.3:1487215-1487234 | None:intergenic | 50.0% | |
| GCTACTGCTGTGTTTGTCGA+TGG | - | chr5.3:1487401-1487420 | MS.gene92284:CDS | 50.0% | |
| GTCTCATCGTCACAAGCCTT+AGG | + | chr5.3:1487446-1487465 | None:intergenic | 50.0% | |
| AACACAGCAGTAGCGAGCTC+CGG | + | chr5.3:1487395-1487414 | None:intergenic | 55.0% | |
| ACTCATCGTCACCGTGCCTA+AGG | - | chr5.3:1487427-1487446 | MS.gene92284:CDS | 55.0% | |
| AGACGCATCACTCGGACATG+TGG | - | chr5.3:1487241-1487260 | MS.gene92284:CDS | 55.0% | |
| AGGATCCTGCTGAACACGTG+AGG | + | chr5.3:1487146-1487165 | None:intergenic | 55.0% | |
| CCACCACCACCAATAGGTTC+AGG | + | chr5.3:1487473-1487492 | None:intergenic | 55.0% | |
| CGTCTGTCTCCGCCACAAAT+CGG | + | chr5.3:1487226-1487245 | None:intergenic | 55.0% | |
| CTCCGCCACAAATCGGAAAC+AGG | + | chr5.3:1487219-1487238 | None:intergenic | 55.0% | |
| GGATCCTGCAACTTCCGCTA+CGG | - | chr5.3:1487159-1487178 | MS.gene92284:CDS | 55.0% | |
| GGATCCTGCTGAACACGTGA+GGG | + | chr5.3:1487145-1487164 | None:intergenic | 55.0% | |
| GGATGCTGACGTGTCAGTTG+AGG | - | chr5.3:1487184-1487203 | MS.gene92284:CDS | 55.0% | |
| !! | CCTGAACCTATTGGTGGTGG+TGG | - | chr5.3:1487470-1487489 | MS.gene92284:CDS | 55.0% |
| !! | GAACCTATTGGTGGTGGTGG+TGG | - | chr5.3:1487473-1487492 | MS.gene92284:CDS | 55.0% |
| ACGTCAGCATCCGACCGTAG+CGG | + | chr5.3:1487176-1487195 | None:intergenic | 60.0% | |
| CCTGCAACTTCCGCTACGGT+CGG | - | chr5.3:1487163-1487182 | MS.gene92284:CDS | 60.0% | |
| GCACCACCACCACCACCAAT+AGG | + | chr5.3:1487479-1487498 | None:intergenic | 60.0% | |
| GCGGAGACAGACGCATCACT+CGG | - | chr5.3:1487233-1487252 | MS.gene92284:CDS | 60.0% | |
| GTCTCCCTCACGTGTTCAGC+AGG | - | chr5.3:1487138-1487157 | MS.gene92284:CDS | 60.0% | |
| ! | CCGACCGTAGCGGAAGTTGC+AGG | + | chr5.3:1487166-1487185 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 1487005 | 1487520 | 1487005 | ID=MS.gene92284 |
| chr5.3 | mRNA | 1487005 | 1487520 | 1487005 | ID=MS.gene92284.t1;Parent=MS.gene92284 |
| chr5.3 | exon | 1487005 | 1487520 | 1487005 | ID=MS.gene92284.t1.exon1;Parent=MS.gene92284.t1 |
| chr5.3 | CDS | 1487005 | 1487520 | 1487005 | ID=cds.MS.gene92284.t1;Parent=MS.gene92284.t1 |
| Gene Sequence |
| Protein sequence |
