Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93744.t1 | XP_003631129.1 | 98.2 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 2.90E-86 | 327.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93744.t1 | G7LE73 | 98.2 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 2.1e-86 | 327.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051651 | MS.gene93744 | 0.804716 | 1.89E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93744.t1 | MTR_8g107420 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 5.36e-122 | 340 |
| MS.gene93744.t1 | MTR_1g108640 | 33.708 | 178 | 98 | 4 | 1 | 165 | 1 | 171 | 3.11e-17 | 75.1 |
| MS.gene93744.t1 | MTR_2g105800 | 38.384 | 99 | 56 | 1 | 14 | 112 | 15 | 108 | 1.70e-15 | 70.1 |
| MS.gene93744.t1 | MTR_5g018720 | 30.303 | 99 | 68 | 1 | 1 | 98 | 1 | 99 | 5.41e-13 | 63.5 |
| MS.gene93744.t1 | MTR_1g087230 | 27.273 | 176 | 113 | 4 | 1 | 161 | 1 | 176 | 3.55e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93744.t1 | AT1G18290 | 37.017 | 181 | 90 | 7 | 1 | 162 | 1 | 176 | 1.27e-30 | 109 |
| MS.gene93744.t1 | AT5G66580 | 34.524 | 84 | 55 | 0 | 15 | 98 | 16 | 99 | 5.83e-13 | 63.2 |
| MS.gene93744.t1 | AT4G37240 | 34.343 | 99 | 64 | 1 | 1 | 98 | 1 | 99 | 1.13e-12 | 62.8 |
| MS.gene93744.t1 | AT3G03280 | 30.539 | 167 | 105 | 4 | 1 | 161 | 1 | 162 | 2.27e-12 | 62.0 |
| MS.gene93744.t1 | AT2G23690 | 33.333 | 84 | 56 | 0 | 15 | 98 | 16 | 99 | 6.58e-11 | 58.2 |
Find 42 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATGCGAGTTTCAACAATT+TGG | 0.189626 | 4.2:+154756 | MS.gene93744:CDS |
| TCCAACCAAAGGGGTGTTTA+AGG | 0.257462 | 4.2:+154480 | MS.gene93744:CDS |
| TTTAAGGTGCTACTCTTAGA+TGG | 0.302251 | 4.2:+154496 | MS.gene93744:CDS |
| CATGCGAGTTTCAACAATTT+GGG | 0.302990 | 4.2:+154757 | MS.gene93744:CDS |
| GGGAGGCTGAACCTGTTAAA+AGG | 0.353755 | 4.2:+154869 | MS.gene93744:CDS |
| TAAGAGCACTCATTTCTTCA+TGG | 0.366168 | 4.2:-154708 | None:intergenic |
| AGTTTCATTTATTGCCTTGA+TGG | 0.393034 | 4.2:-154943 | None:intergenic |
| GGTACTCCAAGCAGAGATCT+TGG | 0.420093 | 4.2:+154890 | MS.gene93744:CDS |
| GAATTCGATGGCCAACTTTG+AGG | 0.420836 | 4.2:-154603 | None:intergenic |
| AAAGTTGGCCATCGAATTCA+AGG | 0.441588 | 4.2:+154607 | MS.gene93744:CDS |
| CCTTGATGGAGTTTCATCAA+TGG | 0.445048 | 4.2:-154929 | None:intergenic |
| TGAGTTCTGCATGTTCCCTT+CGG | 0.456879 | 4.2:+154801 | MS.gene93744:CDS |
| ACCTTAAACACCCCTTTGGT+TGG | 0.462577 | 4.2:-154481 | None:intergenic |
| TAGCACCTTAAACACCCCTT+TGG | 0.471231 | 4.2:-154485 | None:intergenic |
| CTGATAATTATGAGATGCTA+AGG | 0.473011 | 4.2:+154848 | MS.gene93744:CDS |
| AAGAGCACTCATTTCTTCAT+GGG | 0.480087 | 4.2:-154707 | None:intergenic |
| CTTCTTGTACTCCAACCAAA+GGG | 0.491778 | 4.2:+154470 | MS.gene93744:CDS |
| GCGTGTTTCAAGTTCTTCAT+CGG | 0.493614 | 4.2:-154638 | None:intergenic |
| TCATCAATGGTCTCCAATGC+AGG | 0.508093 | 4.2:-154916 | None:intergenic |
| GAGTCACAAACAAACTGTCC+AGG | 0.528192 | 4.2:-154574 | None:intergenic |
| ATTAAGAGCTGCAGAGCTGA+TGG | 0.532721 | 4.2:+154543 | MS.gene93744:CDS |
| GACTCAAGCCACCTCAAAGT+TGG | 0.535144 | 4.2:+154592 | MS.gene93744:CDS |
| GAGATCTTGGAGACCTGCAT+TGG | 0.537320 | 4.2:+154903 | MS.gene93744:CDS |
| GGGTTAACACAGAGTATAGC+AGG | 0.538805 | 4.2:-154687 | None:intergenic |
| ATTGTTGAAACTCGCATGCT+TGG | 0.542497 | 4.2:-154752 | None:intergenic |
| TTAAGGTGCTACTCTTAGAT+GGG | 0.544485 | 4.2:+154497 | MS.gene93744:CDS |
| GTTGAAACTCGCATGCTTGG+TGG | 0.552836 | 4.2:-154749 | None:intergenic |
| ACAGAGTATAGCAGGTCCAT+TGG | 0.559102 | 4.2:-154679 | None:intergenic |
| ACTCTTAGATGGGAGAATAG+AGG | 0.565120 | 4.2:+154507 | MS.gene93744:CDS |
| TTCGATGGCCAACTTTGAGG+TGG | 0.568058 | 4.2:-154600 | None:intergenic |
| CTAATAGTCCTTGAATTCGA+TGG | 0.579984 | 4.2:-154615 | None:intergenic |
| GTTCTGCATGTTCCCTTCGG+AGG | 0.580244 | 4.2:+154804 | MS.gene93744:CDS |
| CTCTTAACTACAAAGCGTCA+AGG | 0.588617 | 4.2:+154725 | MS.gene93744:CDS |
| GCTTCTTGTACTCCAACCAA+AGG | 0.589757 | 4.2:+154469 | MS.gene93744:CDS |
| TCTTAACTACAAAGCGTCAA+GGG | 0.619592 | 4.2:+154726 | MS.gene93744:CDS |
| GAGCTGATGGTAGAATACCC+TGG | 0.631828 | 4.2:+154556 | MS.gene93744:CDS |
| CCATTGATGAAACTCCATCA+AGG | 0.632248 | 4.2:+154929 | MS.gene93744:CDS |
| AGTCACAAACAAACTGTCCA+GGG | 0.643975 | 4.2:-154573 | None:intergenic |
| TGATAATTATGAGATGCTAA+GGG | 0.645988 | 4.2:+154849 | MS.gene93744:CDS |
| AGGTCTCCAAGATCTCTGCT+TGG | 0.669783 | 4.2:-154896 | None:intergenic |
| TAATTATGAGATGCTAAGGG+AGG | 0.689423 | 4.2:+154852 | MS.gene93744:CDS |
| TTCTTGTACTCCAACCAAAG+GGG | 0.691807 | 4.2:+154471 | MS.gene93744:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATAATTATGAGATGCTAA+GGG | + | chr4.2:154849-154868 | MS.gene93744:CDS | 25.0% |
| CTGATAATTATGAGATGCTA+AGG | + | chr4.2:154848-154867 | MS.gene93744:CDS | 30.0% | |
| ! | GTTTTACTTTCTTCTACCAA+TGG | + | chr4.2:154663-154682 | MS.gene93744:CDS | 30.0% |
| ATGCAGAACTCACTAAAAAC+AGG | - | chr4.2:154793-154812 | None:intergenic | 35.0% | |
| CATGCGAGTTTCAACAATTT+GGG | + | chr4.2:154757-154776 | MS.gene93744:CDS | 35.0% | |
| CTAATAGTCCTTGAATTCGA+TGG | - | chr4.2:154618-154637 | None:intergenic | 35.0% | |
| TAATTATGAGATGCTAAGGG+AGG | + | chr4.2:154852-154871 | MS.gene93744:CDS | 35.0% | |
| ! | TCTTAACTACAAAGCGTCAA+GGG | + | chr4.2:154726-154745 | MS.gene93744:CDS | 35.0% |
| !! | AAGAGCACTCATTTCTTCAT+GGG | - | chr4.2:154710-154729 | None:intergenic | 35.0% |
| !! | TAAGAGCACTCATTTCTTCA+TGG | - | chr4.2:154711-154730 | None:intergenic | 35.0% |
| !! | TTAAGGTGCTACTCTTAGAT+GGG | + | chr4.2:154497-154516 | MS.gene93744:CDS | 35.0% |
| !! | TTTAAGGTGCTACTCTTAGA+TGG | + | chr4.2:154496-154515 | MS.gene93744:CDS | 35.0% |
| AAAGTTGGCCATCGAATTCA+AGG | + | chr4.2:154607-154626 | MS.gene93744:CDS | 40.0% | |
| ACTCTTAGATGGGAGAATAG+AGG | + | chr4.2:154507-154526 | MS.gene93744:CDS | 40.0% | |
| AGTCACAAACAAACTGTCCA+GGG | - | chr4.2:154576-154595 | None:intergenic | 40.0% | |
| ATTGTTGAAACTCGCATGCT+TGG | - | chr4.2:154755-154774 | None:intergenic | 40.0% | |
| CCATTGATGAAACTCCATCA+AGG | + | chr4.2:154929-154948 | MS.gene93744:CDS | 40.0% | |
| CCTTGATGGAGTTTCATCAA+TGG | - | chr4.2:154932-154951 | None:intergenic | 40.0% | |
| CTTCTTGTACTCCAACCAAA+GGG | + | chr4.2:154470-154489 | MS.gene93744:CDS | 40.0% | |
| GCATGCGAGTTTCAACAATT+TGG | + | chr4.2:154756-154775 | MS.gene93744:CDS | 40.0% | |
| TTCTTGTACTCCAACCAAAG+GGG | + | chr4.2:154471-154490 | MS.gene93744:CDS | 40.0% | |
| ! | ACTATTTTCTTTGCCTCCGA+AGG | - | chr4.2:154820-154839 | None:intergenic | 40.0% |
| ! | CTATTTTCTTTGCCTCCGAA+GGG | - | chr4.2:154819-154838 | None:intergenic | 40.0% |
| ! | CTCTTAACTACAAAGCGTCA+AGG | + | chr4.2:154725-154744 | MS.gene93744:CDS | 40.0% |
| !! | GCGTGTTTCAAGTTCTTCAT+CGG | - | chr4.2:154641-154660 | None:intergenic | 40.0% |
| !! | TGCTTGGAGTACCTTTTAAC+AGG | - | chr4.2:154883-154902 | None:intergenic | 40.0% |
| ACAGAGTATAGCAGGTCCAT+TGG | - | chr4.2:154682-154701 | None:intergenic | 45.0% | |
| ACCTTAAACACCCCTTTGGT+TGG | - | chr4.2:154484-154503 | None:intergenic | 45.0% | |
| ATTAAGAGCTGCAGAGCTGA+TGG | + | chr4.2:154543-154562 | MS.gene93744:CDS | 45.0% | |
| GAATTCGATGGCCAACTTTG+AGG | - | chr4.2:154606-154625 | None:intergenic | 45.0% | |
| GAGTCACAAACAAACTGTCC+AGG | - | chr4.2:154577-154596 | None:intergenic | 45.0% | |
| GCTTCTTGTACTCCAACCAA+AGG | + | chr4.2:154469-154488 | MS.gene93744:CDS | 45.0% | |
| GGGTTAACACAGAGTATAGC+AGG | - | chr4.2:154690-154709 | None:intergenic | 45.0% | |
| TAGCACCTTAAACACCCCTT+TGG | - | chr4.2:154488-154507 | None:intergenic | 45.0% | |
| TCATCAATGGTCTCCAATGC+AGG | - | chr4.2:154919-154938 | None:intergenic | 45.0% | |
| TGAGTTCTGCATGTTCCCTT+CGG | + | chr4.2:154801-154820 | MS.gene93744:CDS | 45.0% | |
| !! | TCCAACCAAAGGGGTGTTTA+AGG | + | chr4.2:154480-154499 | MS.gene93744:CDS | 45.0% |
| AGGTCTCCAAGATCTCTGCT+TGG | - | chr4.2:154899-154918 | None:intergenic | 50.0% | |
| GACTCAAGCCACCTCAAAGT+TGG | + | chr4.2:154592-154611 | MS.gene93744:CDS | 50.0% | |
| GAGATCTTGGAGACCTGCAT+TGG | + | chr4.2:154903-154922 | MS.gene93744:CDS | 50.0% | |
| GAGCTGATGGTAGAATACCC+TGG | + | chr4.2:154556-154575 | MS.gene93744:CDS | 50.0% | |
| GGGAGGCTGAACCTGTTAAA+AGG | + | chr4.2:154869-154888 | MS.gene93744:CDS | 50.0% | |
| GGTACTCCAAGCAGAGATCT+TGG | + | chr4.2:154890-154909 | MS.gene93744:CDS | 50.0% | |
| GTTGAAACTCGCATGCTTGG+TGG | - | chr4.2:154752-154771 | None:intergenic | 50.0% | |
| ! | TTCGATGGCCAACTTTGAGG+TGG | - | chr4.2:154603-154622 | None:intergenic | 50.0% |
| GTTCTGCATGTTCCCTTCGG+AGG | + | chr4.2:154804-154823 | MS.gene93744:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 154457 | 154957 | 154457 | ID=MS.gene93744 |
| chr4.2 | mRNA | 154457 | 154957 | 154457 | ID=MS.gene93744.t1;Parent=MS.gene93744 |
| chr4.2 | exon | 154457 | 154957 | 154457 | ID=MS.gene93744.t1.exon1;Parent=MS.gene93744.t1 |
| chr4.2 | CDS | 154457 | 154957 | 154457 | ID=cds.MS.gene93744.t1;Parent=MS.gene93744.t1 |
| Gene Sequence |
| Protein sequence |
