AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93747

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93747.t1 MTR_8g107380 91.946 447 34 1 1 445 25 471 0.0 854
MS.gene93747.t1 MTR_8g107380 86.577 447 33 2 1 445 25 446 0.0 790
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93747.t1 AT5G06310 37.892 446 256 9 1 442 23 451 2.46e-86 271
MS.gene93747.t1 AT2G05210 34.848 462 263 15 2 443 23 466 2.55e-73 238
MS.gene93747.t1 AT2G05210 34.848 462 263 15 2 443 23 466 2.55e-73 238
MS.gene93747.t1 AT2G05210 34.848 462 263 15 2 443 23 466 2.55e-73 238
MS.gene93747.t1 AT2G04395 33.645 107 67 3 2 104 23 129 2.89e-11 61.6

Find 93 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTGAGGAATCTGAAAAT+TGG 0.151989 4.2:+182136 None:intergenic
CAAAGAAGGTTACTTAAATT+TGG 0.164890 4.2:-182009 MS.gene93747:CDS
TCAAACCTCGCCCACTATTT+TGG 0.188762 4.2:+179905 None:intergenic
GTGTCCTTAACATTGATAAA+TGG 0.206024 4.2:-180982 MS.gene93747:CDS
ATAATTGGTTTCTCTATGTT+TGG 0.285155 4.2:-181807 MS.gene93747:CDS
CTTGTAATACGTAAAGATTC+AGG 0.289688 4.2:+180686 None:intergenic
ACTTCCCCATACATCAGTTT+TGG 0.291105 4.2:+179950 None:intergenic
GGGAGTCTGCAAAAGATAAT+TGG 0.303591 4.2:-181822 MS.gene93747:CDS
ATCACCCATGGAGGATTTCT+TGG 0.307382 4.2:+179999 None:intergenic
GTAGAATTAATTTCATCTTC+AGG 0.308207 4.2:+181121 None:intergenic
CTAAGTTCACGTGTGTTGTT+CGG 0.336291 4.2:-180378 MS.gene93747:CDS
CACATTGTATATATTACATT+TGG 0.344373 4.2:+181766 None:intergenic
GTGTTTCATCAATCACTCTA+AGG 0.355891 4.2:+182509 None:intergenic
TAAGTTCACGTGTGTTGTTC+GGG 0.356727 4.2:-180377 MS.gene93747:CDS
GTATGGGGAAGTAGAACTTT+TGG 0.363372 4.2:-179939 MS.gene93747:CDS
TAATTGGTTTCTCTATGTTT+GGG 0.364161 4.2:-181806 MS.gene93747:CDS
ACCTCAAGATACTTCATTGC+TGG 0.366802 4.2:-181843 MS.gene93747:intron
CCGTGCCATTAGTGAACTTT+GGG 0.370191 4.2:+182545 None:intergenic
TTGAGAGTGAATTACCTTGC+AGG 0.383097 4.2:+181986 None:intergenic
TGACCCATTTATCAATGTTA+AGG 0.390781 4.2:+180978 None:intergenic
TCCGTGCCATTAGTGAACTT+TGG 0.391027 4.2:+182544 None:intergenic
TCACCCATGGAGGATTTCTT+GGG 0.397379 4.2:+180000 None:intergenic
AATATTTGACACCAAAATAG+TGG 0.401530 4.2:-179916 MS.gene93747:CDS
AGAAAACAGCATTTACTTCC+TGG 0.404273 4.2:+182300 None:intergenic
ATTGCTGTTGTTATTGAAGC+TGG 0.413842 4.2:-182571 MS.gene93747:CDS
CCTTGAGGAATCTGAAAATT+GGG 0.414035 4.2:+182137 None:intergenic
TTTGGCGTTCGATAATTTGA+TGG 0.420640 4.2:+180864 None:intergenic
TTAAAGTGTATGCAGGACTA+TGG 0.426620 4.2:-180937 MS.gene93747:CDS
TGTCCTTAACATTGATAAAT+GGG 0.428681 4.2:-180981 MS.gene93747:CDS
GAACGAATATCGTTGAAATC+AGG 0.431437 4.2:-180731 MS.gene93747:CDS
ATACCTCCAAAACTGATGTA+TGG 0.431794 4.2:-179956 MS.gene93747:CDS
AAAAGTTATGCTCTCCTACT+AGG 0.435695 4.2:-180327 MS.gene93747:CDS
AGGAAGTTAGACAGATTACT+TGG 0.452007 4.2:-180062 MS.gene93747:CDS
TACCTCCAAAACTGATGTAT+GGG 0.462363 4.2:-179955 MS.gene93747:CDS
ATTGTATATATTACATTTGG+TGG 0.462811 4.2:+181769 None:intergenic
TTCTACACGTACATTACATC+CGG 0.466383 4.2:-181104 MS.gene93747:CDS
AGCCTTCCACATGTTTCTCC+TGG 0.470522 4.2:-182439 MS.gene93747:CDS
AGGTTAATCACGATCATGTC+CGG 0.481568 4.2:-180568 MS.gene93747:CDS
TAATATTATCCTCAGAGAAC+AGG 0.485182 4.2:-182195 MS.gene93747:CDS
CTACCGTTGGCTCAATCTTG+AGG 0.486330 4.2:-181042 MS.gene93747:CDS
AATTTGATGGTCCACATTTG+AGG 0.487270 4.2:+180877 None:intergenic
GGTCTCCCAAAGTTCACTAA+TGG 0.489256 4.2:-182550 MS.gene93747:CDS
TTGTATATATTACATTTGGT+GGG 0.496921 4.2:+181770 None:intergenic
TGGTTTCTCTATGTTTGGGA+TGG 0.498067 4.2:-181802 MS.gene93747:CDS
TGTCCGGCATCGTACTTTGA+TGG 0.502519 4.2:-180552 MS.gene93747:CDS
TTACTTAAATTTGGCCTGCA+AGG 0.505621 4.2:-182000 MS.gene93747:intron
TTCTAGCTGTTGGATCCTCT+AGG 0.508462 4.2:+180284 None:intergenic
CGGGCATTGCAAATATGAAA+AGG 0.508721 4.2:-180082 MS.gene93747:CDS
CCTCAAGATACTTCATTGCT+GGG 0.511978 4.2:-181842 MS.gene93747:intron
AAAGTACGTAGTATATCTCT+TGG 0.512985 4.2:+181067 None:intergenic
AAAGCATGGATTCTAGCTGT+TGG 0.520950 4.2:+180274 None:intergenic
CTTCACCAATTACAAAAGCA+TGG 0.524475 4.2:+180260 None:intergenic
TGGTGACGGTATTGTGGTAA+AGG 0.528847 4.2:-180027 MS.gene93747:CDS
CTTGGCAAAGGCATAGGCTC+CGG 0.530366 4.2:+181085 None:intergenic
ATATCTCTTGGCAAAGGCAT+AGG 0.533528 4.2:+181079 None:intergenic
AAATCAGGAACAATTGCTGT+TGG 0.535740 4.2:-180716 MS.gene93747:CDS
ATACATCTTAAAGTGTATGC+AGG 0.538402 4.2:-180944 MS.gene93747:CDS
CGAAGAAGAATGATATCACC+AGG 0.546290 4.2:+182421 None:intergenic
CGGAGATGATCTTCCTCTAT+CGG 0.547270 4.2:+181004 None:intergenic
CTTGTCATATACTTCCTAGT+AGG 0.548949 4.2:+180313 None:intergenic
TGTATATATTACATTTGGTG+GGG 0.549431 4.2:+181771 None:intergenic
TCTAGCTGTTGGATCCTCTA+GGG 0.556090 4.2:+180285 None:intergenic
AAATTGTTAAGCTTAGAAGA+TGG 0.561270 4.2:-182163 MS.gene93747:CDS
GTGTATGCAGGACTATGGTA+TGG 0.567558 4.2:-180932 MS.gene93747:CDS
ACGCTAAGAGAAATCAAAGA+AGG 0.571729 4.2:-182023 MS.gene93747:CDS
GCTCTTTGTCCTGTTCTCTG+AGG 0.572789 4.2:+182186 None:intergenic
TGACACCAAAATAGTGGGCG+AGG 0.575207 4.2:-179910 MS.gene93747:CDS
CCCAAAGTTCACTAATGGCA+CGG 0.581844 4.2:-182545 MS.gene93747:CDS
AGACCCCAAGAAATCCTCCA+TGG 0.582960 4.2:-180004 MS.gene93747:CDS
CACCCATGGAGGATTTCTTG+GGG 0.592410 4.2:+180001 None:intergenic
AATCCTCAAGATTGAGCCAA+CGG 0.594224 4.2:+181039 None:intergenic
GATTCGATATACCTCAAATG+TGG 0.602882 4.2:-180888 MS.gene93747:CDS
ATCAAATTATCGAACGCCAA+AGG 0.604361 4.2:-180862 MS.gene93747:intron
CATCCATCAAAGTACGATGC+CGG 0.606271 4.2:+180549 None:intergenic
GTTCACGTGTGTTGTTCGGG+TGG 0.607327 4.2:-180374 MS.gene93747:CDS
ATATTTGACACCAAAATAGT+GGG 0.607462 4.2:-179915 MS.gene93747:CDS
CGTAGTATATCTCTTGGCAA+AGG 0.607655 4.2:+181073 None:intergenic
ATATCGAATCTTTGATTGTG+AGG 0.610806 4.2:+180901 None:intergenic
GCAGGTAAGAAAACATGACC+AGG 0.610827 4.2:-182318 MS.gene93747:intron
TTAATACAAACTATCACCCA+TGG 0.612169 4.2:+179987 None:intergenic
GGGTGGTAGCAGCAAAGCCA+TGG 0.613819 4.2:-180357 MS.gene93747:CDS
TGAATGTGGTGACGGTATTG+TGG 0.621459 4.2:-180033 MS.gene93747:CDS
GACCCCAAGAAATCCTCCAT+GGG 0.630467 4.2:-180003 MS.gene93747:CDS
ACCTCCAAAACTGATGTATG+GGG 0.632813 4.2:-179954 MS.gene93747:CDS
GGATGTACTCACTCATTCAG+AGG 0.636868 4.2:-180531 MS.gene93747:intron
CACCAGGAGAAACATGTGGA+AGG 0.643881 4.2:+182437 None:intergenic
CTCAAGATTGAGCCAACGGT+AGG 0.647732 4.2:+181043 None:intergenic
ATTTATCAATGTTAAGGACA+CGG 0.661104 4.2:+180984 None:intergenic
ATATCACCAGGAGAAACATG+TGG 0.669575 4.2:+182433 None:intergenic
TTACTTGGAGTAACTGAATG+TGG 0.687106 4.2:-180047 MS.gene93747:CDS
GGAGTAACTGAATGTGGTGA+CGG 0.706321 4.2:-180041 MS.gene93747:CDS
ATACAAACTATCACCCATGG+AGG 0.711847 4.2:+179990 None:intergenic
TATGACAAGACTGACCCTAG+AGG 0.735776 4.2:-180299 MS.gene93747:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAAAAATAATAAATGTTT+AGG - chr4.2:180974-180993 MS.gene93747:CDS 10.0%
!! ACTTCATTTCTTAATATTTA+AGG - chr4.2:180409-180428 MS.gene93747:intron 15.0%
!! TAACAAAAATATAATGACAT+TGG + chr4.2:181713-181732 None:intergenic 15.0%
!! AAAAAATCGTATTTATCCAA+AGG + chr4.2:180563-180582 None:intergenic 20.0%
!! AATTGTATATGGTATCTTAA+CGG - chr4.2:181183-181202 MS.gene93747:intron 20.0%
!! ATTGTATATATTACATTTGG+TGG + chr4.2:180725-180744 None:intergenic 20.0%
!! CACATTGTATATATTACATT+TGG + chr4.2:180728-180747 None:intergenic 20.0%
!! TTGTATATATTACATTTGGT+GGG + chr4.2:180724-180743 None:intergenic 20.0%
!!! AAACATATCAAATTCCTTTT+AGG + chr4.2:181335-181354 None:intergenic 20.0%
!!! ATATATGGTTTGTATAGATT+AGG - chr4.2:181679-181698 MS.gene93747:intron 20.0%
!!! ATTGTATTGTAATTTGTTGA+TGG - chr4.2:180812-180831 MS.gene93747:intron 20.0%
!!! CTACTTTTGCATTATATAAA+GGG - chr4.2:180226-180245 MS.gene93747:intron 20.0%
!!! TAGTTGAAAAAAGTTGATAA+GGG + chr4.2:180278-180297 None:intergenic 20.0%
!!! TCTACTTTTGCATTATATAA+AGG - chr4.2:180225-180244 MS.gene93747:intron 20.0%
!!! TTAGTTGAAAAAAGTTGATA+AGG + chr4.2:180279-180298 None:intergenic 20.0%
! AAATCCTAATTCCTGAAATA+AGG + chr4.2:181057-181076 None:intergenic 25.0%
! AAATTGTTAAGCTTAGAAGA+TGG - chr4.2:180328-180347 MS.gene93747:CDS 25.0%
! AACATGAATTCTTGCTAATA+TGG - chr4.2:181109-181128 MS.gene93747:CDS 25.0%
! AATATTTGACACCAAAATAG+TGG - chr4.2:182575-182594 MS.gene93747:CDS 25.0%
! AATCCTAATTCCTGAAATAA+GGG + chr4.2:181056-181075 None:intergenic 25.0%
! ATATGTTTGTTTATGAATGC+AGG - chr4.2:181347-181366 MS.gene93747:intron 25.0%
! ATATTATGACTCCACTAATT+TGG + chr4.2:181661-181680 None:intergenic 25.0%
! ATATTTGACACCAAAATAGT+GGG - chr4.2:182576-182595 MS.gene93747:CDS 25.0%
! ATTTATCAATGTTAAGGACA+CGG + chr4.2:181510-181529 None:intergenic 25.0%
! CAAAGAAGGTTACTTAAATT+TGG - chr4.2:180482-180501 MS.gene93747:intron 25.0%
! CAGCAACAAAAAAACATTTA+AGG + chr4.2:180613-180632 None:intergenic 25.0%
! CATAATATTGATCATTGCAT+AGG + chr4.2:182030-182049 None:intergenic 25.0%
! GAATGATATTGTTGAAGAAA+AGG + chr4.2:180139-180158 None:intergenic 25.0%
! GTAGAATTAATTTCATCTTC+AGG + chr4.2:181373-181392 None:intergenic 25.0%
! TAACTGCTAAGAATTGTATA+TGG - chr4.2:181172-181191 MS.gene93747:intron 25.0%
! TAGTGGAGTCATAATATATA+TGG - chr4.2:181664-181683 MS.gene93747:intron 25.0%
! TATACTCGTTCATTTATTGA+AGG - chr4.2:182084-182103 MS.gene93747:intron 25.0%
! TATTGTTGAAGAAAAGGAAA+AGG + chr4.2:180133-180152 None:intergenic 25.0%
! TGTCCTTAACATTGATAAAT+GGG - chr4.2:181510-181529 MS.gene93747:intron 25.0%
! TTTATGTTTGCAATCCTAAA+AGG - chr4.2:181318-181337 MS.gene93747:intron 25.0%
!! ATAATTGGTTTCTCTATGTT+TGG - chr4.2:180684-180703 MS.gene93747:intron 25.0%
!! ATTCTTTTTCTCTATTTTGC+AGG - chr4.2:180155-180174 MS.gene93747:intron 25.0%
!! TAATTGGTTTCTCTATGTTT+GGG - chr4.2:180685-180704 MS.gene93747:intron 25.0%
!! TGTATATATTACATTTGGTG+GGG + chr4.2:180723-180742 None:intergenic 25.0%
!! TTACACAATGTACTTTTCAA+AGG - chr4.2:180876-180895 MS.gene93747:CDS 25.0%
!!! AAAAACAATAGTGTTTTGAG+AGG + chr4.2:182056-182075 None:intergenic 25.0%
!!! AAAACAATAGTGTTTTGAGA+GGG + chr4.2:182055-182074 None:intergenic 25.0%
!!! TAAAGTTTTAGCTTTCCTTA+CGG - chr4.2:181872-181891 MS.gene93747:intron 25.0%
AAAAGCTAAGAACATACGTA+CGG + chr4.2:180104-180123 None:intergenic 30.0%
AAAGTACGTAGTATATCTCT+TGG + chr4.2:181427-181446 None:intergenic 30.0%
AATAAAACGGTCAAGATTGA+AGG - chr4.2:181028-181047 MS.gene93747:CDS 30.0%
ATACATCTTAAAGTGTATGC+AGG - chr4.2:181547-181566 MS.gene93747:intron 30.0%
ATATCGAATCTTTGATTGTG+AGG + chr4.2:181593-181612 None:intergenic 30.0%
CTTAGCGTAGAAAAAGTAAA+TGG + chr4.2:180456-180475 None:intergenic 30.0%
CTTGTAATACGTAAAGATTC+AGG + chr4.2:181808-181827 None:intergenic 30.0%
GAAATGAGTTAAATACCTTG+AGG + chr4.2:180372-180391 None:intergenic 30.0%
GATACCTGCAAGTAATAAAA+CGG - chr4.2:181015-181034 MS.gene93747:CDS 30.0%
GTGTCCTTAACATTGATAAA+TGG - chr4.2:181509-181528 MS.gene93747:intron 30.0%
TAATATTATCCTCAGAGAAC+AGG - chr4.2:180296-180315 MS.gene93747:CDS 30.0%
TAGAAAATTCACTGACAACT+CGG - chr4.2:181086-181105 MS.gene93747:CDS 30.0%
TGAAGAAAAGGAAAAGGAAA+AGG + chr4.2:180127-180146 None:intergenic 30.0%
TGACCCATTTATCAATGTTA+AGG + chr4.2:181516-181535 None:intergenic 30.0%
TGTAAGGATAAAACACAGTT+TGG + chr4.2:181902-181921 None:intergenic 30.0%
TTAATACAAACTATCACCCA+TGG + chr4.2:182507-182526 None:intergenic 30.0%
! AGAATCCATGCTTTTGTAAT+TGG - chr4.2:182226-182245 MS.gene93747:CDS 30.0%
! CTTTTGTAATTGGTGAAGAT+GGG - chr4.2:182236-182255 MS.gene93747:CDS 30.0%
! TACTATCAGTTTAAGACCTT+TGG - chr4.2:180544-180563 MS.gene93747:CDS 30.0%
! TTTTATTACTTGCAGGTATC+CGG + chr4.2:181015-181034 None:intergenic 30.0%
! TTTTGTAATTGGTGAAGATG+GGG - chr4.2:182237-182256 MS.gene93747:CDS 30.0%
!! TTTTACTTGTTACCTTTCCA+AGG - chr4.2:180580-180599 MS.gene93747:intron 30.0%
!!! AAAGTTTTAGCTTTCCTTAC+GGG - chr4.2:181873-181892 MS.gene93747:intron 30.0%
AAAAAGAGTCTCCTGTCATA+GGG + chr4.2:182379-182398 None:intergenic 35.0%
AAAAGTTATGCTCTCCTACT+AGG - chr4.2:182164-182183 MS.gene93747:CDS 35.0%
AAAGGCAAGTTACTTGACAT+AGG - chr4.2:180894-180913 MS.gene93747:CDS 35.0%
AAAGGTGGTTACCAAATTAG+TGG - chr4.2:181647-181666 MS.gene93747:intron 35.0%
AAATCAGGAACAATTGCTGT+TGG - chr4.2:181775-181794 MS.gene93747:CDS 35.0%
AATTCTTGCTAATATGGCCA+TGG - chr4.2:181115-181134 MS.gene93747:CDS 35.0%
ACATTTAAGGATCAGAACCT+TGG + chr4.2:180600-180619 None:intergenic 35.0%
ACCTTGAGGAATCTGAAAAT+TGG + chr4.2:180358-180377 None:intergenic 35.0%
ACGCTAAGAGAAATCAAAGA+AGG - chr4.2:180468-180487 MS.gene93747:intron 35.0%
AGAAAACAGCATTTACTTCC+TGG + chr4.2:180194-180213 None:intergenic 35.0%
AGGAAGTTAGACAGATTACT+TGG - chr4.2:182429-182448 MS.gene93747:CDS 35.0%
ATACCTCCAAAACTGATGTA+TGG - chr4.2:182535-182554 MS.gene93747:CDS 35.0%
ATCAAATTATCGAACGCCAA+AGG - chr4.2:181629-181648 MS.gene93747:intron 35.0%
CAAGTTACTTGACATAGGAT+AGG - chr4.2:180899-180918 MS.gene93747:CDS 35.0%
CCTTGAGGAATCTGAAAATT+GGG + chr4.2:180357-180376 None:intergenic 35.0%
CTTCACCAATTACAAAAGCA+TGG + chr4.2:182234-182253 None:intergenic 35.0%
CTTGTCATATACTTCCTAGT+AGG + chr4.2:182181-182200 None:intergenic 35.0%
GAACGAATATCGTTGAAATC+AGG - chr4.2:181760-181779 MS.gene93747:intron 35.0%
GATTCGATATACCTCAAATG+TGG - chr4.2:181603-181622 MS.gene93747:intron 35.0%
GTGTTTCATCAATCACTCTA+AGG + chr4.2:179985-180004 None:intergenic 35.0%
TACAATTCTTAGCAGTTAGC+TGG + chr4.2:181170-181189 None:intergenic 35.0%
TACCTCCAAAACTGATGTAT+GGG - chr4.2:182536-182555 MS.gene93747:CDS 35.0%
TATTCGTTCATCATACAACC+TGG + chr4.2:181749-181768 None:intergenic 35.0%
TGTTATGTTTGTGTTGAACC+AGG - chr4.2:181728-181747 MS.gene93747:intron 35.0%
TTAAAGTGTATGCAGGACTA+TGG - chr4.2:181554-181573 MS.gene93747:intron 35.0%
TTACTTAAATTTGGCCTGCA+AGG - chr4.2:180491-180510 MS.gene93747:intron 35.0%
TTACTTGGAGTAACTGAATG+TGG - chr4.2:182444-182463 MS.gene93747:CDS 35.0%
TTCTACACGTACATTACATC+CGG - chr4.2:181387-181406 MS.gene93747:intron 35.0%
TTTATTACTTGCAGGTATCC+GGG + chr4.2:181014-181033 None:intergenic 35.0%
TTTGGCGTTCGATAATTTGA+TGG + chr4.2:181630-181649 None:intergenic 35.0%
! AATTTGATGGTCCACATTTG+AGG + chr4.2:181617-181636 None:intergenic 35.0%
! ACTAATTTGGTAACCACCTT+TGG + chr4.2:181648-181667 None:intergenic 35.0%
! ACTGTGTTTTATCCTTACAG+AGG - chr4.2:181903-181922 MS.gene93747:intron 35.0%
! ATTGAAGGTTCCCTTATTTC+AGG - chr4.2:181043-181062 MS.gene93747:CDS 35.0%
! ATTGCTGTTGTTATTGAAGC+TGG - chr4.2:179920-179939 MS.gene93747:CDS 35.0%
! CTAATATGGCCATGGATTTT+TGG - chr4.2:181123-181142 MS.gene93747:CDS 35.0%
! CTTTTCATATTTGCAATGCC+CGG + chr4.2:182411-182430 None:intergenic 35.0%
! GCTTTTGTAATTGGTGAAGA+TGG - chr4.2:182235-182254 MS.gene93747:CDS 35.0%
! GTTCCCTTATTTCAGGAATT+AGG - chr4.2:181050-181069 MS.gene93747:CDS 35.0%
! TACTTTTGAACAACCGATAG+AGG - chr4.2:181474-181493 MS.gene93747:intron 35.0%
! TTTTATTCAAACCCACCACA+TGG + chr4.2:180793-180812 None:intergenic 35.0%
! TTTTCATATTTGCAATGCCC+GGG + chr4.2:182410-182429 None:intergenic 35.0%
!! TTGACCGTTTTATTACTTGC+AGG + chr4.2:181022-181041 None:intergenic 35.0%
AAATTATCGAACGCCAAAGG+TGG - chr4.2:181632-181651 MS.gene93747:intron 40.0%
AATCCTCAAGATTGAGCCAA+CGG + chr4.2:181455-181474 None:intergenic 40.0%
ACCTCAAGATACTTCATTGC+TGG - chr4.2:180648-180667 MS.gene93747:intron 40.0%
ACCTCCAAAACTGATGTATG+GGG - chr4.2:182537-182556 MS.gene93747:CDS 40.0%
AGAATCAGCCACACAGAATA+TGG + chr4.2:182341-182360 None:intergenic 40.0%
AGGTTAATCACGATCATGTC+CGG - chr4.2:181923-181942 MS.gene93747:intron 40.0%
ATACAAACTATCACCCATGG+AGG + chr4.2:182504-182523 None:intergenic 40.0%
ATATCACCAGGAGAAACATG+TGG + chr4.2:180061-180080 None:intergenic 40.0%
CAAAAAGAGTCTCCTGTCAT+AGG + chr4.2:182380-182399 None:intergenic 40.0%
CCTCAAGATACTTCATTGCT+GGG - chr4.2:180649-180668 MS.gene93747:intron 40.0%
CGAAGAAGAATGATATCACC+AGG + chr4.2:180073-180092 None:intergenic 40.0%
CGTAGTATATCTCTTGGCAA+AGG + chr4.2:181421-181440 None:intergenic 40.0%
CTGCTATAATACCCTATGAC+AGG - chr4.2:182365-182384 MS.gene93747:intron 40.0%
GATCGTGATTAACCTCTGTA+AGG + chr4.2:181918-181937 None:intergenic 40.0%
GGGAGTCTGCAAAAGATAAT+TGG - chr4.2:180669-180688 MS.gene93747:intron 40.0%
GTAACCATCCATCTTGAGAT+TGG - chr4.2:181214-181233 MS.gene93747:intron 40.0%
TAAGGATCAGAACCTTGGAA+AGG + chr4.2:180595-180614 None:intergenic 40.0%
TGGTTTCTCTATGTTTGGGA+TGG - chr4.2:180689-180708 MS.gene93747:CDS 40.0%
TTGAGAGTGAATTACCTTGC+AGG + chr4.2:180508-180527 None:intergenic 40.0%
TTTGTCGACCATATTCTGTG+TGG - chr4.2:182330-182349 MS.gene93747:intron 40.0%
! AAAGCATGGATTCTAGCTGT+TGG + chr4.2:182220-182239 None:intergenic 40.0%
! ACTTCCCCATACATCAGTTT+TGG + chr4.2:182544-182563 None:intergenic 40.0%
! ATATCTCTTGGCAAAGGCAT+AGG + chr4.2:181415-181434 None:intergenic 40.0%
! ATCTCTTGTTGATTGAGCAG+TGG - chr4.2:180837-180856 MS.gene93747:intron 40.0%
! CATAACTTTTCAGCTTGCCA+TGG + chr4.2:182154-182173 None:intergenic 40.0%
! CCCAATTTTCAGATTCCTCA+AGG - chr4.2:180354-180373 MS.gene93747:CDS 40.0%
! CGGGCATTGCAAATATGAAA+AGG - chr4.2:182409-182428 MS.gene93747:intron 40.0%
! CTAAGTTCACGTGTGTTGTT+CGG - chr4.2:182113-182132 MS.gene93747:intron 40.0%
! GTATGGGGAAGTAGAACTTT+TGG - chr4.2:182552-182571 MS.gene93747:CDS 40.0%
! TAAGTTCACGTGTGTTGTTC+GGG - chr4.2:182114-182133 MS.gene93747:intron 40.0%
! TGACAGGAGACTCTTTTTGA+TGG - chr4.2:182381-182400 MS.gene93747:intron 40.0%
!! ACTCTTTTTGATGGTTACCC+GGG - chr4.2:182390-182409 MS.gene93747:intron 40.0%
!! CATAGGTTGATGTCAAGTGT+GGG + chr4.2:182013-182032 None:intergenic 40.0%
!! GACTCTTTTTGATGGTTACC+CGG - chr4.2:182389-182408 MS.gene93747:intron 40.0%
AAACCCACCACATGGATTCA+GGG + chr4.2:180785-180804 None:intergenic 45.0%
AATCGCCTGCCAAAAATCCA+TGG + chr4.2:181135-181154 None:intergenic 45.0%
ACACAGTTTGGAAACCCGTA+AGG + chr4.2:181890-181909 None:intergenic 45.0%
ATCACCCATGGAGGATTTCT+TGG + chr4.2:182495-182514 None:intergenic 45.0%
CACTCCAATCTCAAGATGGA+TGG + chr4.2:181221-181240 None:intergenic 45.0%
CATCCATCAAAGTACGATGC+CGG + chr4.2:181945-181964 None:intergenic 45.0%
CCCAAAGTTCACTAATGGCA+CGG - chr4.2:179946-179965 MS.gene93747:CDS 45.0%
CGGAGATGATCTTCCTCTAT+CGG + chr4.2:181490-181509 None:intergenic 45.0%
GCAACACTCCAATCTCAAGA+TGG + chr4.2:181225-181244 None:intergenic 45.0%
GCAGGTAAGAAAACATGACC+AGG - chr4.2:180173-180192 MS.gene93747:intron 45.0%
GGAGTAACTGAATGTGGTGA+CGG - chr4.2:182450-182469 MS.gene93747:CDS 45.0%
GGATGTACTCACTCATTCAG+AGG - chr4.2:181960-181979 MS.gene93747:intron 45.0%
GGTCTCCCAAAGTTCACTAA+TGG - chr4.2:179941-179960 MS.gene93747:CDS 45.0%
GTGTATGCAGGACTATGGTA+TGG - chr4.2:181559-181578 MS.gene93747:intron 45.0%
TATGACAAGACTGACCCTAG+AGG - chr4.2:182192-182211 MS.gene93747:CDS 45.0%
TCACCCATGGAGGATTTCTT+GGG + chr4.2:182494-182513 None:intergenic 45.0%
TCTAGCTGTTGGATCCTCTA+GGG + chr4.2:182209-182228 None:intergenic 45.0%
! CCCAGCAATGAAGTATCTTG+AGG + chr4.2:180652-180671 None:intergenic 45.0%
! CCGTGCCATTAGTGAACTTT+GGG + chr4.2:179949-179968 None:intergenic 45.0%
! CTACGTACTTTTCCTACCGT+TGG - chr4.2:181436-181455 MS.gene93747:intron 45.0%
! TCAAACCTCGCCCACTATTT+TGG + chr4.2:182589-182608 None:intergenic 45.0%
! TCCGTGCCATTAGTGAACTT+TGG + chr4.2:179950-179969 None:intergenic 45.0%
! TTCTAGCTGTTGGATCCTCT+AGG + chr4.2:182210-182229 None:intergenic 45.0%
!! GCATAGGTTGATGTCAAGTG+TGG + chr4.2:182014-182033 None:intergenic 45.0%
!! TCCCCATACATCAGTTTTGG+AGG + chr4.2:182541-182560 None:intergenic 45.0%
!! TGAATGTGGTGACGGTATTG+TGG - chr4.2:182458-182477 MS.gene93747:CDS 45.0%
!! TGGTGACGGTATTGTGGTAA+AGG - chr4.2:182464-182483 MS.gene93747:CDS 45.0%
!!! TATGGCCATGGATTTTTGGC+AGG - chr4.2:181127-181146 MS.gene93747:intron 45.0%
AACCCACCACATGGATTCAG+GGG + chr4.2:180784-180803 None:intergenic 50.0%
ACTTCTCCCCTGAATCCATG+TGG - chr4.2:180775-180794 MS.gene93747:intron 50.0%
AGACCCCAAGAAATCCTCCA+TGG - chr4.2:182487-182506 MS.gene93747:CDS 50.0%
AGCCTTCCACATGTTTCTCC+TGG - chr4.2:180052-180071 MS.gene93747:CDS 50.0%
CAAACCCACCACATGGATTC+AGG + chr4.2:180786-180805 None:intergenic 50.0%
CACCAGGAGAAACATGTGGA+AGG + chr4.2:180057-180076 None:intergenic 50.0%
CACCCATGGAGGATTTCTTG+GGG + chr4.2:182493-182512 None:intergenic 50.0%
CTCAAGATTGAGCCAACGGT+AGG + chr4.2:181451-181470 None:intergenic 50.0%
GACCCCAAGAAATCCTCCAT+GGG - chr4.2:182488-182507 MS.gene93747:CDS 50.0%
GCTCTTTGTCCTGTTCTCTG+AGG + chr4.2:180308-180327 None:intergenic 50.0%
TGTCCGGCATCGTACTTTGA+TGG - chr4.2:181939-181958 MS.gene93747:intron 50.0%
!! CTACCGTTGGCTCAATCTTG+AGG - chr4.2:181449-181468 MS.gene93747:intron 50.0%
!! TGACACCAAAATAGTGGGCG+AGG - chr4.2:182581-182600 MS.gene93747:CDS 50.0%
CTCCCCTGAATCCATGTGGT+GGG - chr4.2:180779-180798 MS.gene93747:intron 55.0%
GCGAAGAATCTACAGCACGC+AGG + chr4.2:181273-181292 None:intergenic 55.0%
TAGGTTCTCCGCAAGTGTCC+CGG - chr4.2:180993-181012 MS.gene93747:CDS 55.0%
TCTCCCCTGAATCCATGTGG+TGG - chr4.2:180778-180797 MS.gene93747:intron 55.0%
! CTTGGCAAAGGCATAGGCTC+CGG + chr4.2:181409-181428 None:intergenic 55.0%
! GTTCACGTGTGTTGTTCGGG+TGG - chr4.2:182117-182136 MS.gene93747:intron 55.0%
GCAGGTATCCGGGACACTTG+CGG + chr4.2:181004-181023 None:intergenic 60.0%
GGGTGGTAGCAGCAAAGCCA+TGG - chr4.2:182134-182153 MS.gene93747:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 179905 182608 179905 ID=MS.gene93747
chr4.2 mRNA 179905 182608 179905 ID=MS.gene93747.t1;Parent=MS.gene93747
chr4.2 exon 182411 182608 182411 ID=MS.gene93747.t1.exon1;Parent=MS.gene93747.t1
chr4.2 CDS 182411 182608 182411 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 182138 182336 182138 ID=MS.gene93747.t1.exon2;Parent=MS.gene93747.t1
chr4.2 CDS 182138 182336 182138 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 182001 182062 182001 ID=MS.gene93747.t1.exon3;Parent=MS.gene93747.t1
chr4.2 CDS 182001 182062 182001 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 181769 181857 181769 ID=MS.gene93747.t1.exon4;Parent=MS.gene93747.t1
chr4.2 CDS 181769 181857 181769 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 180863 181144 180863 ID=MS.gene93747.t1.exon5;Parent=MS.gene93747.t1
chr4.2 CDS 180863 181144 180863 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 180687 180763 180687 ID=MS.gene93747.t1.exon6;Parent=MS.gene93747.t1
chr4.2 CDS 180687 180763 180687 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 180532 180590 180532 ID=MS.gene93747.t1.exon7;Parent=MS.gene93747.t1
chr4.2 CDS 180532 180590 180532 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 180255 180407 180255 ID=MS.gene93747.t1.exon8;Parent=MS.gene93747.t1
chr4.2 CDS 180255 180407 180255 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
chr4.2 exon 179905 180126 179905 ID=MS.gene93747.t1.exon9;Parent=MS.gene93747.t1
chr4.2 CDS 179905 180126 179905 ID=cds.MS.gene93747.t1;Parent=MS.gene93747.t1
Gene Sequence

>MS.gene93747

ATGAAAGTTAATATCATTGCTGTTGTTATTGAAGCTGGTCTCCCAAAGTTCACTAATGGCACGGATCAATGTTGTAACCTTAGAGTGATTGATGAAACACGTTACGAAACATCTATCTCTGTTAACTTGTTTGCTCAAACTGCTCAAAGCCTTCCACATGTTTCTCCTGGTGATATCATTCTTCTTCGTAATGTTACCGTACGTATGTTCTTAGCTTTTCCTTTTCCTTTTCCTTTTCTTCAACAATATCATTCTTTTTCTCTATTTTGCAGGTAAGAAAACATGACCAGGAAGTAAATGCTGTTTTCTACAAAGACTCTTCTACTTTTGCATTATATAAAGGGAAAGATACTGCTCTTCACGATTTTGACCCTTATCAACTTTTTTCAACTAATATTATCCTCAGAGAACAGGACAAAGAGCAAATTGTTAAGCTTAGAAGATGGTTACCCAATTTTCAGATTCCTCAAGGTATTTAACTCATTTCATTGCTGATTTCATAATACTTCATTTCTTAATATTTAAGGTTTTTTATATCTTGTGTAGAACCATTTACTTTTTCTACGCTAAGAGAAATCAAAGAAGGTTACTTAAATTTGGCCTGCAAGGTAATTCACTCTCAATTAGTTTTGATATTTATACTATCAGTTTAAGACCTTTGGATAAATACGATTTTTTTACTTGTTACCTTTCCAAGGTTCTGATCCTTAAATGTTTTTTTGTTGCTGTTGTTGTTGTTTTACACCTCAAGATACTTCATTGCTGGGAGTCTGCAAAAGATAATTGGTTTCTCTATGTTTGGGATGGTACAGATACCCCACCAAATGTAATATATACAATGTGAGTTACTTTCATATTGACTGTTCAACTACTTCTCCCCTGAATCCATGTGGTGGGTTTGAATAAAATTGTATTGTAATTTGTTGATGGAAATCTCTTGTTGATTGAGCAGTGGTGCTTTCTAGTATTATTTACACAATGTACTTTTCAAAGGCAAGTTACTTGACATAGGATAGGCATATGTATAAATTTATAAATGAGAAATTGTGTTTTATTGATCTCTAAAGCTTTGAAAAATAATAAATGTTTAGGTTCTCCGCAAGTGTCCCGGATACCTGCAAGTAATAAAACGGTCAAGATTGAAGGTTCCCTTATTTCAGGAATTAGGATTTGAATTGAAATAGAAAATTCACTGACAACTCGGAACATGAATTCTTGCTAATATGGCCATGGATTTTTGGCAGGCGATTCTGTTGAAAAAACCAGCTAACTGCTAAGAATTGTATATGGTATCTTAACGGCAGCATCTGTAACCATCCATCTTGAGATTGGAGTGTTGCTCTTTTTCATTTAATCAGTTTCAAGCCTGCGTGCTGTAGATTCTTCGCAATCTGTCTGTACAGAATACATAAATTTATGTTTGCAATCCTAAAAGGAATTTGATATGTTTGTTTATGAATGCAGGCCTGAAGATGAAATTAATTCTACACGTACATTACATCCGGAGCCTATGCCTTTGCCAAGAGATATACTACGTACTTTTCCTACCGTTGGCTCAATCTTGAGGATTACTTTTGAACAACCGATAGAGGAAGATCATCTCCGTGTCCTTAACATTGATAAATGGGTCAAGTTTGTCAATATACATCTTAAAGTGTATGCAGGACTATGGTATGGCGTTTTTACCTCACAATCAAAGATTCGATATACCTCAAATGTGGACCATCAAATTATCGAACGCCAAAGGTGGTTACCAAATTAGTGGAGTCATAATATATATGGTTTGTATAGATTAGGTCAGTTAACCAATGTCATTATATTTTTGTTATGTTTGTGTTGAACCAGGTTGTATGATGAACGAATATCGTTGAAATCAGGAACAATTGCTGTTGGCAACTTACCTGAATCTTTACGTATTACAAGTAATAATTCATTTTTCTTACACTTGTACTAAACTTCTATATCACTAAAGTTTTAGCTTTCCTTACGGGTTTCCAAACTGTGTTTTATCCTTACAGAGGTTAATCACGATCATGTCCGGCATCGTACTTTGATGGATGTACTCACTCATTCAGAGGTACTATGCTGCACTTTTGTCACTTGTACCCACACTTGACATCAACCTATGCAATGATCAATATTATGTGCCCTCTCAAAACACTATTGTTTTTTTGCTTAGTATACTCGTTCATTTATTGAAGGTCACAGCTAAGTTCACGTGTGTTGTTCGGGTGGTAGCAGCAAAGCCATGGCAAGCTGAAAAGTTATGCTCTCCTACTAGGAAGTATATGACAAGACTGACCCTAGAGGATCCAACAGCTAGAATCCATGCTTTTGTAATTGGTGAAGATGGGGTCAGAACAAATTTTATCGTCATCAATTTTAGAATATACATGTTTCTAATTATTTATTATTCGTCTGTTTATTTGTCGACCATATTCTGTGTGGCTGATTCTTTTTCTGCTATAATACCCTATGACAGGAGACTCTTTTTGATGGTTACCCGGGCATTGCAAATATGAAAAGGAAGTTAGACAGATTACTTGGAGTAACTGAATGTGGTGACGGTATTGTGGTAAAGGAGACCCCAAGAAATCCTCCATGGGTGATAGTTTGTATTAAATCATACTATACCTCCAAAACTGATGTATGGGGAAGTAGAACTTTTGGAATATTTGACACCAAAATAGTGGGCGAGGTTTGA
Protein sequence

>MS.gene93747.t1

MKVNIIAVVIEAGLPKFTNGTDQCCNLRVIDETRYETSISVNLFAQTAQSLPHVSPGDIILLRNVTVRKHDQEVNAVFYKDSSTFALYKGKDTALHDFDPYQLFSTNIILREQDKEQIVKLRRWLPNFQIPQEPFTFSTLREIKEGYLNLACKILHCWESAKDNWFLYVWDGTDTPPNVIYTMPEDEINSTRTLHPEPMPLPRDILRTFPTVGSILRITFEQPIEEDHLRVLNIDKWVKFVNIHLKVYAGLWYGVFTSQSKIRYTSNVDHQIIERQRLYDERISLKSGTIAVGNLPESLRITKVNHDHVRHRTLMDVLTHSEVTAKFTCVVRVVAAKPWQAEKLCSPTRKYMTRLTLEDPTARIHAFVIGEDGETLFDGYPGIANMKRKLDRLLGVTECGDGIVVKETPRNPPWVIVCIKSYYTSKTDVWGSRTFGIFDTKIVGEV