Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94053.t1 | XP_013445197.1 | 99.3 | 274 | 2 | 0 | 1 | 274 | 68 | 341 | 1.90E-151 | 545 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94053.t1 | Q84ST4 | 84.7 | 274 | 42 | 0 | 1 | 274 | 70 | 343 | 4.8e-135 | 481.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94053.t1 | A0A072TQT8 | 99.3 | 274 | 2 | 0 | 1 | 274 | 68 | 341 | 1.4e-151 | 545.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052982 | MS.gene94053 | 0.811852 | 5.69E-51 | -1.69E-46 |
| MS.gene05602 | MS.gene94053 | 0.856244 | 3.67E-62 | -1.69E-46 |
| MS.gene056497 | MS.gene94053 | 0.814054 | 1.87E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene94053 | 0.818219 | 2.20E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94053.t1 | MTR_8g044240 | 99.270 | 274 | 2 | 0 | 1 | 274 | 68 | 341 | 0.0 | 571 |
| MS.gene94053.t1 | MTR_6g088500 | 45.528 | 246 | 114 | 4 | 4 | 234 | 180 | 420 | 1.01e-62 | 206 |
| MS.gene94053.t1 | MTR_6g088500 | 46.948 | 213 | 94 | 3 | 4 | 201 | 180 | 388 | 8.82e-56 | 184 |
| MS.gene94053.t1 | MTR_4g058810 | 29.798 | 198 | 133 | 4 | 7 | 202 | 16 | 209 | 9.64e-17 | 78.2 |
| MS.gene94053.t1 | MTR_4g058840 | 29.798 | 198 | 133 | 4 | 7 | 202 | 16 | 209 | 4.68e-16 | 76.3 |
| MS.gene94053.t1 | MTR_4g058820 | 29.798 | 198 | 133 | 4 | 7 | 202 | 16 | 209 | 4.68e-16 | 76.3 |
| MS.gene94053.t1 | MTR_7g063260 | 28.713 | 202 | 134 | 5 | 2 | 202 | 16 | 208 | 8.56e-16 | 75.5 |
| MS.gene94053.t1 | MTR_7g063270 | 28.571 | 203 | 135 | 5 | 8 | 209 | 22 | 215 | 1.83e-15 | 74.7 |
| MS.gene94053.t1 | MTR_3g068085 | 28.342 | 187 | 127 | 5 | 2 | 187 | 15 | 195 | 1.95e-15 | 74.7 |
| MS.gene94053.t1 | MTR_7g105590 | 28.272 | 191 | 128 | 3 | 8 | 198 | 14 | 195 | 2.58e-15 | 72.8 |
| MS.gene94053.t1 | MTR_3g085740 | 29.310 | 232 | 140 | 9 | 7 | 227 | 80 | 298 | 5.37e-15 | 73.9 |
| MS.gene94053.t1 | MTR_7g063390 | 29.064 | 203 | 128 | 7 | 2 | 200 | 16 | 206 | 5.49e-15 | 73.2 |
| MS.gene94053.t1 | MTR_7g063360 | 29.268 | 205 | 133 | 6 | 2 | 204 | 16 | 210 | 1.77e-14 | 72.0 |
| MS.gene94053.t1 | MTR_4g134660 | 31.658 | 199 | 124 | 8 | 7 | 202 | 11 | 200 | 1.12e-13 | 69.3 |
| MS.gene94053.t1 | MTR_4g052320 | 28.497 | 193 | 129 | 3 | 8 | 200 | 14 | 197 | 1.73e-13 | 68.9 |
| MS.gene94053.t1 | MTR_3g068070 | 27.000 | 200 | 136 | 5 | 2 | 200 | 15 | 205 | 3.03e-13 | 68.6 |
| MS.gene94053.t1 | MTR_4g132850 | 27.461 | 193 | 127 | 6 | 8 | 199 | 13 | 193 | 4.87e-13 | 67.8 |
| MS.gene94053.t1 | MTR_3g068065 | 27.723 | 202 | 130 | 6 | 2 | 199 | 17 | 206 | 8.99e-13 | 67.0 |
| MS.gene94053.t1 | MTR_4g052300 | 27.461 | 193 | 131 | 3 | 8 | 200 | 14 | 197 | 9.78e-13 | 67.0 |
| MS.gene94053.t1 | MTR_1g051165 | 26.500 | 200 | 136 | 5 | 7 | 199 | 19 | 214 | 1.40e-12 | 66.6 |
| MS.gene94053.t1 | MTR_7g063330 | 29.902 | 204 | 117 | 9 | 8 | 202 | 21 | 207 | 2.19e-12 | 65.9 |
| MS.gene94053.t1 | MTR_6g023590 | 26.425 | 193 | 133 | 4 | 8 | 199 | 19 | 203 | 8.04e-12 | 64.3 |
| MS.gene94053.t1 | MTR_7g107670 | 26.667 | 195 | 133 | 4 | 8 | 202 | 39 | 223 | 1.27e-11 | 63.9 |
| MS.gene94053.t1 | MTR_6g041980 | 35.165 | 91 | 59 | 0 | 2 | 92 | 64 | 154 | 1.27e-11 | 63.9 |
| MS.gene94053.t1 | MTR_7g063320 | 27.411 | 197 | 131 | 7 | 8 | 202 | 21 | 207 | 1.49e-11 | 63.5 |
| MS.gene94053.t1 | MTR_3g086210 | 25.943 | 212 | 138 | 9 | 8 | 213 | 57 | 255 | 2.24e-11 | 63.5 |
| MS.gene94053.t1 | MTR_4g078630 | 26.020 | 196 | 130 | 4 | 8 | 201 | 25 | 207 | 2.41e-11 | 63.2 |
| MS.gene94053.t1 | MTR_3g068155 | 26.087 | 184 | 129 | 4 | 2 | 184 | 19 | 196 | 3.23e-11 | 62.8 |
| MS.gene94053.t1 | MTR_6g041980 | 35.165 | 91 | 59 | 0 | 2 | 92 | 64 | 154 | 3.86e-11 | 63.2 |
| MS.gene94053.t1 | MTR_6g023910 | 26.154 | 195 | 135 | 4 | 8 | 201 | 19 | 205 | 5.61e-11 | 62.0 |
| MS.gene94053.t1 | MTR_6g023610 | 26.442 | 208 | 142 | 5 | 8 | 212 | 19 | 218 | 6.00e-11 | 62.0 |
| MS.gene94053.t1 | MTR_3g068175 | 27.222 | 180 | 120 | 5 | 8 | 184 | 23 | 194 | 9.17e-11 | 61.2 |
| MS.gene94053.t1 | MTR_4g064883 | 24.286 | 210 | 143 | 6 | 8 | 216 | 20 | 214 | 9.50e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94053.t1 | AT5G04900 | 83.577 | 274 | 44 | 1 | 1 | 274 | 76 | 348 | 1.36e-177 | 493 |
| MS.gene94053.t1 | AT4G13250 | 45.122 | 246 | 115 | 4 | 4 | 234 | 161 | 401 | 1.88e-61 | 202 |
| MS.gene94053.t1 | AT4G13250 | 44.309 | 246 | 115 | 5 | 4 | 234 | 161 | 399 | 2.19e-58 | 194 |
| MS.gene94053.t1 | AT1G10310 | 33.333 | 198 | 118 | 6 | 7 | 202 | 20 | 205 | 2.04e-17 | 79.7 |
| MS.gene94053.t1 | AT5G65205 | 31.429 | 245 | 141 | 8 | 7 | 243 | 11 | 236 | 3.83e-17 | 79.7 |
| MS.gene94053.t1 | AT1G24360 | 31.818 | 198 | 117 | 7 | 7 | 199 | 79 | 263 | 9.87e-17 | 79.0 |
| MS.gene94053.t1 | AT5G10050 | 32.039 | 206 | 121 | 7 | 7 | 211 | 10 | 197 | 3.06e-16 | 77.0 |
| MS.gene94053.t1 | AT4G05530 | 28.421 | 190 | 125 | 5 | 8 | 197 | 15 | 193 | 2.45e-12 | 65.9 |
| MS.gene94053.t1 | AT3G47360 | 28.846 | 208 | 137 | 7 | 7 | 213 | 50 | 247 | 5.75e-12 | 65.1 |
| MS.gene94053.t1 | AT5G50690 | 28.421 | 190 | 125 | 6 | 7 | 195 | 50 | 229 | 9.59e-12 | 63.9 |
| MS.gene94053.t1 | AT5G50590 | 28.421 | 190 | 125 | 6 | 7 | 195 | 50 | 229 | 9.59e-12 | 63.9 |
| MS.gene94053.t1 | AT5G50690 | 27.059 | 255 | 160 | 10 | 7 | 253 | 50 | 286 | 1.32e-11 | 63.9 |
| MS.gene94053.t1 | AT5G50590 | 27.059 | 255 | 160 | 10 | 7 | 253 | 50 | 286 | 1.32e-11 | 63.9 |
| MS.gene94053.t1 | AT4G13180 | 25.121 | 207 | 148 | 5 | 8 | 212 | 19 | 220 | 2.04e-11 | 63.2 |
| MS.gene94053.t1 | AT5G06060 | 27.941 | 204 | 129 | 6 | 2 | 200 | 9 | 199 | 3.77e-11 | 62.4 |
| MS.gene94053.t1 | AT1G52340 | 26.667 | 195 | 138 | 2 | 8 | 202 | 24 | 213 | 5.88e-11 | 62.0 |
Find 60 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACTTAACTGAAAATATT+TGG | 0.084717 | 8.4:-56249525 | None:intergenic |
| AAGAGCTTGAGAGAAGAATT+TGG | 0.202687 | 8.4:+56244639 | MS.gene94053:CDS |
| AACTGAAATACATTGATATA+TGG | 0.208099 | 8.4:+56244850 | MS.gene94053:CDS |
| GGTTTCATTGATCTGTTGTT+AGG | 0.239754 | 8.4:-56249463 | None:intergenic |
| TTTCTTAGGTTTGCTGCATA+TGG | 0.270591 | 8.4:+56246218 | MS.gene94053:intron |
| AGAGCTTGAGAGAAGAATTT+GGG | 0.296966 | 8.4:+56244640 | MS.gene94053:CDS |
| TTACTTAACTGAAAATATTT+GGG | 0.311967 | 8.4:-56249524 | None:intergenic |
| AACTGAAAATATTTGGGAAT+AGG | 0.347590 | 8.4:-56249518 | None:intergenic |
| AAGATCTGTTGTAACCATTC+CGG | 0.358289 | 8.4:-56249006 | None:intergenic |
| TCATTTGACAAAGTCATTAC+AGG | 0.364217 | 8.4:+56246262 | MS.gene94053:CDS |
| TTAGGGATAGATCTAATGTT+TGG | 0.372199 | 8.4:-56249445 | None:intergenic |
| AGATCTGTTGTAACCATTCC+GGG | 0.381265 | 8.4:-56249005 | None:intergenic |
| TGTCATGGTTCACAATCTTT+CGG | 0.414123 | 8.4:+56247134 | MS.gene94053:CDS |
| CTTTCAAACCCGTGAGGAAC+CGG | 0.418163 | 8.4:-56249495 | None:intergenic |
| TTCGCAGGTTGCAGAATACT+TGG | 0.425684 | 8.4:+56249420 | MS.gene94053:intron |
| ACTGAAATACATTGATATAT+GGG | 0.428120 | 8.4:+56244851 | MS.gene94053:CDS |
| TACCTTATGATTTAGGGATA+GGG | 0.435637 | 8.4:+56243878 | MS.gene94053:intron |
| ATCATACAGAGATTTGCATT+TGG | 0.440755 | 8.4:+56249981 | MS.gene94053:intron |
| CATTTGGCGCAAGGAGGAAC+CGG | 0.441028 | 8.4:+56249997 | MS.gene94053:CDS |
| CCAGTAATCAAAACGTTGTA+AGG | 0.443126 | 8.4:-56243005 | None:intergenic |
| GTTTCATTGATCTGTTGTTA+GGG | 0.444901 | 8.4:-56249462 | None:intergenic |
| TTTAGGGATAGGGTATGCTT+TGG | 0.462514 | 8.4:+56243888 | MS.gene94053:intron |
| GCGAAGGAATTTCTAAAAGC+AGG | 0.468485 | 8.4:+56243910 | MS.gene94053:CDS |
| GTCTGGCGCCAATACAAAAC+AGG | 0.469357 | 8.4:+56249034 | MS.gene94053:CDS |
| ACAACAGATCTTCTCATGTC+TGG | 0.474724 | 8.4:+56249017 | MS.gene94053:CDS |
| TATTTAGGCAATAAAGATGA+TGG | 0.480267 | 8.4:+56245698 | MS.gene94053:intron |
| GGTATTCATTTATAGATGAA+AGG | 0.481392 | 8.4:+56244601 | MS.gene94053:intron |
| TTCAATCTTCAATAATGTAC+CGG | 0.490213 | 8.4:-56250016 | None:intergenic |
| GTATTCATTTATAGATGAAA+GGG | 0.523248 | 8.4:+56244602 | MS.gene94053:intron |
| TCATCTGATGCTTCAGCAAG+TGG | 0.524655 | 8.4:-56245465 | None:intergenic |
| ATTGACGGGGCAGGTTCAGA+TGG | 0.528992 | 8.4:+56245753 | MS.gene94053:CDS |
| AGAGATTTGCATTTGGCGCA+AGG | 0.529215 | 8.4:+56249988 | MS.gene94053:intron |
| TTGATTACTGGCTCAACCAA+AGG | 0.533805 | 8.4:+56243017 | MS.gene94053:CDS |
| CAATGAAACCGACATACATC+CGG | 0.538906 | 8.4:+56249476 | MS.gene94053:CDS |
| GACATACATCCGGTTCCTCA+CGG | 0.547192 | 8.4:+56249486 | MS.gene94053:CDS |
| AAATATGACCTCCGCGAGGT+TGG | 0.556067 | 8.4:-56245725 | None:intergenic |
| ATGGTGTAGATCAATAATGC+TGG | 0.558885 | 8.4:+56245420 | MS.gene94053:intron |
| CAGATGGAAGACCAACTCCC+AGG | 0.564488 | 8.4:+56245769 | MS.gene94053:CDS |
| TTTGGTGTTACAGCCCGGAA+TGG | 0.571021 | 8.4:+56248992 | MS.gene94053:intron |
| ACATACATCCGGTTCCTCAC+GGG | 0.571767 | 8.4:+56249487 | MS.gene94053:CDS |
| GTGGTTACAACAAACACACT+TGG | 0.597765 | 8.4:+56245581 | MS.gene94053:CDS |
| CTTGGCTGAACCAGCCGAAG+TGG | 0.599008 | 8.4:+56249146 | MS.gene94053:CDS |
| AATTTGGGGAGCAGCATGTG+TGG | 0.600569 | 8.4:+56244655 | MS.gene94053:CDS |
| AACGTTGTAAGGAGGAAGCA+TGG | 0.614243 | 8.4:-56242994 | None:intergenic |
| GTAATCAAAACGTTGTAAGG+AGG | 0.617451 | 8.4:-56243002 | None:intergenic |
| GAGCTTGAGAGAAGAATTTG+GGG | 0.620456 | 8.4:+56244641 | MS.gene94053:CDS |
| TTAGGCAATAAAGATGATGG+TGG | 0.629231 | 8.4:+56245701 | MS.gene94053:intron |
| ATTTGGGGAGCAGCATGTGT+GGG | 0.636962 | 8.4:+56244656 | MS.gene94053:CDS |
| TAGGCAATAAAGATGATGGT+GGG | 0.647346 | 8.4:+56245702 | MS.gene94053:intron |
| GATAGGGTATGCTTTGGCGA+AGG | 0.648888 | 8.4:+56243894 | MS.gene94053:CDS |
| ACTTGCTGAAGCATCAGATG+AGG | 0.650746 | 8.4:+56245467 | MS.gene94053:CDS |
| GTGAGGAACCGGATGTATGT+CGG | 0.655366 | 8.4:-56249484 | None:intergenic |
| TGGAGCAACAAAGAGAAGTG+TGG | 0.673454 | 8.4:+56246238 | MS.gene94053:CDS |
| ATTCTGTGGATGCAGTGAGG+TGG | 0.684617 | 8.4:+56245562 | MS.gene94053:intron |
| ATGATGGTGGGCCAACCTCG+CGG | 0.691592 | 8.4:+56245714 | MS.gene94053:CDS |
| GATTTGCATTTGGCGCAAGG+AGG | 0.693001 | 8.4:+56249991 | MS.gene94053:CDS |
| ATGGTGGGCCAACCTCGCGG+AGG | 0.726382 | 8.4:+56245717 | MS.gene94053:CDS |
| CTTGATGATATGCTGTCGAG+AGG | 0.741135 | 8.4:+56245604 | MS.gene94053:CDS |
| AATAGGCTTTCAAACCCGTG+AGG | 0.759601 | 8.4:-56249501 | None:intergenic |
| TTGAAAATATGACCTCCGCG+AGG | 0.783707 | 8.4:-56245729 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 56242996 | 56250037 | 56242996 | ID=MS.gene94053 |
| chr8.4 | mRNA | 56242996 | 56250037 | 56242996 | ID=MS.gene94053.t1;Parent=MS.gene94053 |
| chr8.4 | exon | 56242996 | 56243038 | 56242996 | ID=MS.gene94053.t1.exon1;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56242996 | 56243038 | 56242996 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56243893 | 56243961 | 56243893 | ID=MS.gene94053.t1.exon2;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56243893 | 56243961 | 56243893 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56244616 | 56244677 | 56244616 | ID=MS.gene94053.t1.exon3;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56244616 | 56244677 | 56244616 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56244786 | 56244872 | 56244786 | ID=MS.gene94053.t1.exon4;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56244786 | 56244872 | 56244786 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56245429 | 56245496 | 56245429 | ID=MS.gene94053.t1.exon5;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56245429 | 56245496 | 56245429 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56245577 | 56245625 | 56245577 | ID=MS.gene94053.t1.exon6;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56245577 | 56245625 | 56245577 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56245705 | 56245790 | 56245705 | ID=MS.gene94053.t1.exon7;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56245705 | 56245790 | 56245705 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56246226 | 56246283 | 56246226 | ID=MS.gene94053.t1.exon8;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56246226 | 56246283 | 56246226 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56247105 | 56247155 | 56247105 | ID=MS.gene94053.t1.exon9;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56247105 | 56247155 | 56247105 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56249005 | 56249055 | 56249005 | ID=MS.gene94053.t1.exon10;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56249005 | 56249055 | 56249005 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56249126 | 56249167 | 56249126 | ID=MS.gene94053.t1.exon11;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56249126 | 56249167 | 56249126 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56249427 | 56249537 | 56249427 | ID=MS.gene94053.t1.exon12;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56249427 | 56249537 | 56249427 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| chr8.4 | exon | 56249990 | 56250037 | 56249990 | ID=MS.gene94053.t1.exon13;Parent=MS.gene94053.t1 |
| chr8.4 | CDS | 56249990 | 56250037 | 56249990 | ID=cds.MS.gene94053.t1;Parent=MS.gene94053.t1 |
| Gene Sequence |
| Protein sequence |
