Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94181.t1 | XP_003616743.2 | 97.4 | 155 | 4 | 0 | 1 | 155 | 25 | 179 | 6.00E-73 | 283.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94181.t1 | G7K7C1 | 97.4 | 155 | 4 | 0 | 1 | 155 | 25 | 179 | 4.3e-73 | 283.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055133 | MS.gene94181 | 0.801195 | 1.01E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94181.t1 | MTR_5g083810 | 97.419 | 155 | 4 | 0 | 1 | 155 | 25 | 179 | 8.76e-109 | 307 |
| MS.gene94181.t1 | MTR_3g466150 | 46.835 | 158 | 82 | 1 | 1 | 156 | 1 | 158 | 8.20e-46 | 147 |
| MS.gene94181.t1 | MTR_7g072470 | 59.350 | 123 | 49 | 1 | 27 | 149 | 6 | 127 | 1.25e-43 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94181.t1 | AT2G40435 | 64.103 | 156 | 54 | 2 | 1 | 155 | 1 | 155 | 1.00e-67 | 202 |
| MS.gene94181.t1 | AT3G56220 | 57.419 | 155 | 63 | 3 | 1 | 153 | 1 | 154 | 2.97e-57 | 176 |
| MS.gene94181.t1 | AT2G40435 | 64.341 | 129 | 45 | 1 | 1 | 128 | 1 | 129 | 6.81e-56 | 172 |
| MS.gene94181.t1 | AT2G40435 | 65.354 | 127 | 43 | 1 | 1 | 126 | 1 | 127 | 8.12e-56 | 171 |
| MS.gene94181.t1 | AT3G56220 | 57.937 | 126 | 50 | 3 | 1 | 124 | 1 | 125 | 1.95e-43 | 140 |
| MS.gene94181.t1 | AT1G29270 | 34.752 | 141 | 83 | 6 | 1 | 135 | 1 | 138 | 4.90e-12 | 60.5 |
Find 22 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAATTACCATAGGCAAGT+TGG | 0.394555 | 5.4:+66336052 | None:intergenic |
| TCAATTACCATAGGCAAGTT+GGG | 0.413845 | 5.4:+66336053 | None:intergenic |
| TACAGTGGAAACCCTAGAAA+AGG | 0.442701 | 5.4:-66335446 | MS.gene94181:CDS |
| GCTTACTGTGCTGGAAGCTA+GGG | 0.457026 | 5.4:-66335344 | MS.gene94181:CDS |
| CTTTGCAATTTACACTTGAT+CGG | 0.474337 | 5.4:+66334770 | None:intergenic |
| TATTGAAAATCTAAAGGAAA+AGG | 0.484456 | 5.4:-66336129 | MS.gene94181:CDS |
| AGAGATGAGGCTTACTGTGC+TGG | 0.485736 | 5.4:-66335353 | MS.gene94181:CDS |
| GGCTTACTGTGCTGGAAGCT+AGG | 0.491888 | 5.4:-66335345 | MS.gene94181:CDS |
| CCACAATCCCAACTTGCCTA+TGG | 0.504234 | 5.4:-66336060 | MS.gene94181:intron |
| AAAGTATATTGAAAATCTAA+AGG | 0.516255 | 5.4:-66336135 | MS.gene94181:CDS |
| AGTAAGCCTCATCTCTTCAA+TGG | 0.521158 | 5.4:+66335360 | None:intergenic |
| CCATAGGCAAGTTGGGATTG+TGG | 0.527764 | 5.4:+66336060 | None:intergenic |
| ACAGTGGAAACCCTAGAAAA+GGG | 0.541907 | 5.4:-66335445 | MS.gene94181:CDS |
| TTACCTCTCCTCCAACAGCT+TGG | 0.548657 | 5.4:+66335296 | None:intergenic |
| TTTCCAAGCTGTTGGAGGAG+AGG | 0.549162 | 5.4:-66335299 | MS.gene94181:intron |
| TTGAAGCCATTGAAGAGATG+AGG | 0.583640 | 5.4:-66335366 | MS.gene94181:CDS |
| TGATGCATCGATTACGATCG+AGG | 0.609202 | 5.4:+66336157 | None:intergenic |
| GTATTGAAACAAGCATACCT+TGG | 0.629414 | 5.4:+66335389 | None:intergenic |
| CAAGCTGTGAAACAAGCAGT+GGG | 0.658275 | 5.4:-66334822 | MS.gene94181:CDS |
| TGGACTTGTGCAGGTTACAG+TGG | 0.658893 | 5.4:-66335461 | MS.gene94181:intron |
| CTTATGTAGAGTGAAGATCA+AGG | 0.661626 | 5.4:-66334861 | MS.gene94181:intron |
| ACAAGCTGTGAAACAAGCAG+TGG | 0.701888 | 5.4:-66334823 | MS.gene94181:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAAAAAATATATAAAT+GGG | + | chr5.4:66335585-66335604 | None:intergenic | 0.0% |
| !! | AAGACTATATATTATTATTA+GGG | + | chr5.4:66336610-66336629 | None:intergenic | 10.0% |
| !! | ACTTTCTTAATAAAAAATAT+TGG | + | chr5.4:66335611-66335630 | None:intergenic | 10.0% |
| !! | ATTAATTCCAATAATAAAAA+TGG | + | chr5.4:66335005-66335024 | None:intergenic | 10.0% |
| !! | AAAGTATATTGAAAATCTAA+AGG | - | chr5.4:66335463-66335482 | MS.gene94181:intron | 15.0% |
| !! | AACTTATAAACAATTAAACA+CGG | - | chr5.4:66335813-66335832 | MS.gene94181:intron | 15.0% |
| !! | GAAGACTATATATTATTATT+AGG | + | chr5.4:66336611-66336630 | None:intergenic | 15.0% |
| !!! | GAATATTCTTTTATAGAATT+GGG | + | chr5.4:66334945-66334964 | None:intergenic | 15.0% |
| !!! | TGAATATTCTTTTATAGAAT+TGG | + | chr5.4:66334946-66334965 | None:intergenic | 15.0% |
| !! | ACTAAAAAAAATAGCAAACT+TGG | + | chr5.4:66335032-66335051 | None:intergenic | 20.0% |
| !! | ATATAAAAACGTTACTCATT+TGG | - | chr5.4:66336501-66336520 | MS.gene94181:intron | 20.0% |
| !! | TATAAAAACGTTACTCATTT+GGG | - | chr5.4:66336502-66336521 | MS.gene94181:intron | 20.0% |
| !! | TATTGAAAATCTAAAGGAAA+AGG | - | chr5.4:66335469-66335488 | MS.gene94181:intron | 20.0% |
| !! | TTATTATTAGGGTTAAGATT+AGG | + | chr5.4:66336599-66336618 | None:intergenic | 20.0% |
| !! | TTTCATTGAGAATATATTGA+TGG | + | chr5.4:66334919-66334938 | None:intergenic | 20.0% |
| !!! | GAAATTTGAAATGGATTTTA+TGG | - | chr5.4:66336113-66336132 | MS.gene94181:CDS | 20.0% |
| !!! | GAGTAACGTTTTTATATAAA+GGG | + | chr5.4:66336498-66336517 | None:intergenic | 20.0% |
| !!! | GCATATCAATTAAATTTTCA+TGG | - | chr5.4:66336394-66336413 | MS.gene94181:intron | 20.0% |
| !!! | TGAGTAACGTTTTTATATAA+AGG | + | chr5.4:66336499-66336518 | None:intergenic | 20.0% |
| !!! | TTTTCTAATACTTCTTCTTA+TGG | - | chr5.4:66335384-66335403 | MS.gene94181:CDS | 20.0% |
| ! | AATGGAAAGAAAAGAAAGAT+AGG | + | chr5.4:66334886-66334905 | None:intergenic | 25.0% |
| ! | ACAAAAATATTGACAAGTGA+AGG | + | chr5.4:66336461-66336480 | None:intergenic | 25.0% |
| ! | ATAAAAACGTTACTCATTTG+GGG | - | chr5.4:66336503-66336522 | MS.gene94181:intron | 25.0% |
| ! | GCAGAAAGAATGAATTAATA+AGG | + | chr5.4:66335106-66335125 | None:intergenic | 25.0% |
| ! | GTACATTGAATCATTGATAT+TGG | - | chr5.4:66336552-66336571 | MS.gene94181:intron | 25.0% |
| ! | TGCAAGTATGAAATTTGAAA+TGG | - | chr5.4:66336104-66336123 | MS.gene94181:CDS | 25.0% |
| ! | TTAAGATTAGGCATCATTTA+TGG | + | chr5.4:66336587-66336606 | None:intergenic | 25.0% |
| !! | AATTCTGCTTCAAGTTATTT+AGG | + | chr5.4:66335966-66335985 | None:intergenic | 25.0% |
| !! | ATTCTGCTTCAAGTTATTTA+GGG | + | chr5.4:66335965-66335984 | None:intergenic | 25.0% |
| !! | GTAATTTTCTCAATTACCAT+AGG | + | chr5.4:66335557-66335576 | None:intergenic | 25.0% |
| !! | TATAAGAAATCCCTTTTCTA+GGG | + | chr5.4:66336166-66336185 | None:intergenic | 25.0% |
| !! | TATCTTTCTTTTCTTTCCAT+TGG | - | chr5.4:66334885-66334904 | MS.gene94181:intron | 25.0% |
| !! | TTATAAGAAATCCCTTTTCT+AGG | + | chr5.4:66336167-66336186 | None:intergenic | 25.0% |
| !! | TTTTCGTTAACTATCTTGAA+GGG | - | chr5.4:66335768-66335787 | MS.gene94181:intron | 25.0% |
| !!! | GTCTCTTTGAATTGTTTTAT+TGG | + | chr5.4:66335908-66335927 | None:intergenic | 25.0% |
| !!! | TCACTTTTTTTCTTCTTCTT+GGG | - | chr5.4:66335989-66336008 | MS.gene94181:intron | 25.0% |
| !!! | TTCACTTTTTTTCTTCTTCT+TGG | - | chr5.4:66335988-66336007 | MS.gene94181:intron | 25.0% |
| !!! | TTTGAAATGGATTTTATGGA+TGG | - | chr5.4:66336117-66336136 | MS.gene94181:CDS | 25.0% |
| !!! | TTTGGGTCCATTTTTATTAT+TGG | - | chr5.4:66334995-66335014 | MS.gene94181:intron | 25.0% |
| AATAAAAATGGACCCAAATC+AGG | + | chr5.4:66334993-66335012 | None:intergenic | 30.0% | |
| CACATAGCTGATAAGAATAT+AGG | + | chr5.4:66335336-66335355 | None:intergenic | 30.0% | |
| CTATATTCTTATCAGCTATG+TGG | - | chr5.4:66335334-66335353 | MS.gene94181:CDS | 30.0% | |
| GCTGATAAGAATATAGGATA+AGG | + | chr5.4:66335330-66335349 | None:intergenic | 30.0% | |
| TATACTCTTGTTCCTGATTT+GGG | - | chr5.4:66334978-66334997 | MS.gene94181:intron | 30.0% | |
| TATGTGGAGCTTATTTGATA+TGG | - | chr5.4:66335350-66335369 | MS.gene94181:CDS | 30.0% | |
| TGGGACAAGCAATAAAAAAT+TGG | - | chr5.4:66336795-66336814 | MS.gene94181:CDS | 30.0% | |
| ! | AATCAGAATTTGACTGCTTT+TGG | - | chr5.4:66335641-66335660 | MS.gene94181:intron | 30.0% |
| ! | CTTCTTATGGTTTTTATGCA+TGG | - | chr5.4:66335397-66335416 | MS.gene94181:CDS | 30.0% |
| ! | GTTTTCGTTAACTATCTTGA+AGG | - | chr5.4:66335767-66335786 | MS.gene94181:intron | 30.0% |
| ! | TATGGTTTTTATGCATGGTT+TGG | - | chr5.4:66335402-66335421 | MS.gene94181:CDS | 30.0% |
| !! | AATTTTTGTTTCCAAGCTGT+TGG | - | chr5.4:66336291-66336310 | MS.gene94181:intron | 30.0% |
| !!! | TTTTCTAGGATAGATAGACA+AGG | + | chr5.4:66335934-66335953 | None:intergenic | 30.0% |
| AGATAGGAGATAGAAAATGC+AGG | + | chr5.4:66334870-66334889 | None:intergenic | 35.0% | |
| AGGATACACATACACTACTA+TGG | + | chr5.4:66335310-66335329 | None:intergenic | 35.0% | |
| ATTGATGGAGAGAAATCCAA+TGG | + | chr5.4:66334904-66334923 | None:intergenic | 35.0% | |
| CTATACTCTTGTTCCTGATT+TGG | - | chr5.4:66334977-66334996 | MS.gene94181:intron | 35.0% | |
| CTTATGTAGAGTGAAGATCA+AGG | - | chr5.4:66336737-66336756 | MS.gene94181:intron | 35.0% | |
| GATAGGAGATAGAAAATGCA+GGG | + | chr5.4:66334869-66334888 | None:intergenic | 35.0% | |
| GTATTGAAACAAGCATACCT+TGG | + | chr5.4:66336212-66336231 | None:intergenic | 35.0% | |
| TCAATTACCATAGGCAAGTT+GGG | + | chr5.4:66335548-66335567 | None:intergenic | 35.0% | |
| TCGTTATTGATGAACTTAGC+TGG | + | chr5.4:66336660-66336679 | None:intergenic | 35.0% | |
| TGATTCAATGTACAGCAACT+CGG | + | chr5.4:66336545-66336564 | None:intergenic | 35.0% | |
| TTAACAATCTCACAAGCGTT+AGG | - | chr5.4:66335864-66335883 | MS.gene94181:intron | 35.0% | |
| TTGTTAATATCGCGAGCTAA+TGG | + | chr5.4:66335851-66335870 | None:intergenic | 35.0% | |
| TTTGAACCTAGATATGCATG+TGG | + | chr5.4:66335161-66335180 | None:intergenic | 35.0% | |
| ! | GGATGAGAGCATAAAAGTAT+AGG | + | chr5.4:66335140-66335159 | None:intergenic | 35.0% |
| !! | TTAGGGTCAAGACTTTTTCT+AGG | + | chr5.4:66335948-66335967 | None:intergenic | 35.0% |
| ACAGTGGAAACCCTAGAAAA+GGG | - | chr5.4:66336153-66336172 | MS.gene94181:CDS | 40.0% | |
| AGTAAGCCTCATCTCTTCAA+TGG | + | chr5.4:66336241-66336260 | None:intergenic | 40.0% | |
| CCATCATTCTCATGCATGAT+GGG | + | chr5.4:66336704-66336723 | None:intergenic | 40.0% | |
| CTAGGATAGATAGACAAGGA+TGG | + | chr5.4:66335930-66335949 | None:intergenic | 40.0% | |
| CTCAATTACCATAGGCAAGT+TGG | + | chr5.4:66335549-66335568 | None:intergenic | 40.0% | |
| TACAGTGGAAACCCTAGAAA+AGG | - | chr5.4:66336152-66336171 | MS.gene94181:CDS | 40.0% | |
| TCCATCATTCTCATGCATGA+TGG | + | chr5.4:66336705-66336724 | None:intergenic | 40.0% | |
| TCTCATCCACATGCATATCT+AGG | - | chr5.4:66335152-66335171 | MS.gene94181:intron | 40.0% | |
| TTGAAGCCATTGAAGAGATG+AGG | - | chr5.4:66336232-66336251 | MS.gene94181:intron | 40.0% | |
| ! | ATTTGGGGTTCTCGAGAAAA+GGG | - | chr5.4:66336518-66336537 | MS.gene94181:intron | 40.0% |
| ! | GAGCATAAAAGTATAGGCAG+TGG | + | chr5.4:66335134-66335153 | None:intergenic | 40.0% |
| ! | TTTTCAGCAAAAAGCAGCCA+AGG | - | chr5.4:66336192-66336211 | MS.gene94181:intron | 40.0% |
| !! | TTTTATGGATGGACTTGTGC+AGG | - | chr5.4:66336128-66336147 | MS.gene94181:CDS | 40.0% |
| !! | TTTTGTTTCCAAGCTGTTGG+AGG | - | chr5.4:66336294-66336313 | MS.gene94181:intron | 40.0% |
| ACAAGCTGTGAAACAAGCAG+TGG | - | chr5.4:66336775-66336794 | MS.gene94181:CDS | 45.0% | |
| CAAGCTGTGAAACAAGCAGT+GGG | - | chr5.4:66336776-66336795 | MS.gene94181:CDS | 45.0% | |
| CCCATCATGCATGAGAATGA+TGG | - | chr5.4:66336701-66336720 | MS.gene94181:intron | 45.0% | |
| CTCACAAGCGTTAGGTTAGT+AGG | - | chr5.4:66335872-66335891 | MS.gene94181:intron | 45.0% | |
| ! | CATTTGGGGTTCTCGAGAAA+AGG | - | chr5.4:66336517-66336536 | MS.gene94181:intron | 45.0% |
| !! | TGATGCATCGATTACGATCG+AGG | + | chr5.4:66335444-66335463 | None:intergenic | 45.0% |
| !! | CTATAAAAAAAATATATAAA+TGG | + | chr5.4:66335586-66335605 | None:intergenic | 5.0% |
| AGAGATGAGGCTTACTGTGC+TGG | - | chr5.4:66336245-66336264 | MS.gene94181:intron | 50.0% | |
| CCACAATCCCAACTTGCCTA+TGG | - | chr5.4:66335538-66335557 | MS.gene94181:intron | 50.0% | |
| GCTTACTGTGCTGGAAGCTA+GGG | - | chr5.4:66336254-66336273 | MS.gene94181:intron | 50.0% | |
| TGGACTTGTGCAGGTTACAG+TGG | - | chr5.4:66336137-66336156 | MS.gene94181:CDS | 50.0% | |
| TTACCTCTCCTCCAACAGCT+TGG | + | chr5.4:66336305-66336324 | None:intergenic | 50.0% | |
| TTTCCAAGCTGTTGGAGGAG+AGG | - | chr5.4:66336299-66336318 | MS.gene94181:intron | 50.0% | |
| ! | CCATAGGCAAGTTGGGATTG+TGG | + | chr5.4:66335541-66335560 | None:intergenic | 50.0% |
| GGCTTACTGTGCTGGAAGCT+AGG | - | chr5.4:66336253-66336272 | MS.gene94181:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 66334779 | 66336841 | 66334779 | ID=MS.gene94181 |
| chr5.4 | mRNA | 66334779 | 66336841 | 66334779 | ID=MS.gene94181.t1;Parent=MS.gene94181 |
| chr5.4 | exon | 66336764 | 66336841 | 66336764 | ID=MS.gene94181.t1.exon1;Parent=MS.gene94181.t1 |
| chr5.4 | CDS | 66336764 | 66336841 | 66336764 | ID=cds.MS.gene94181.t1;Parent=MS.gene94181.t1 |
| chr5.4 | exon | 66336061 | 66336189 | 66336061 | ID=MS.gene94181.t1.exon2;Parent=MS.gene94181.t1 |
| chr5.4 | CDS | 66336061 | 66336189 | 66336061 | ID=cds.MS.gene94181.t1;Parent=MS.gene94181.t1 |
| chr5.4 | exon | 66335300 | 66335470 | 66335300 | ID=MS.gene94181.t1.exon3;Parent=MS.gene94181.t1 |
| chr5.4 | CDS | 66335300 | 66335470 | 66335300 | ID=cds.MS.gene94181.t1;Parent=MS.gene94181.t1 |
| chr5.4 | exon | 66334779 | 66334874 | 66334779 | ID=MS.gene94181.t1.exon4;Parent=MS.gene94181.t1 |
| chr5.4 | CDS | 66334779 | 66334874 | 66334779 | ID=cds.MS.gene94181.t1;Parent=MS.gene94181.t1 |
| Gene Sequence |
| Protein sequence |
