Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94192.t1 | XP_003616732.1 | 100 | 113 | 0 | 0 | 1 | 113 | 1 | 113 | 2.90E-61 | 244.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94192.t1 | Q94AR4 | 48.5 | 66 | 26 | 2 | 12 | 75 | 10 | 69 | 1.5e-10 | 67.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94192.t1 | G7K7B0 | 100.0 | 113 | 0 | 0 | 1 | 113 | 1 | 113 | 2.1e-61 | 244.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058041 | MS.gene94192 | -0.816478 | 5.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94192.t1 | MTR_5g083690 | 99.123 | 114 | 1 | 0 | 1 | 114 | 1 | 114 | 3.93e-82 | 236 |
| MS.gene94192.t1 | MTR_3g023110 | 56.000 | 50 | 22 | 0 | 4 | 53 | 1 | 50 | 1.72e-15 | 68.6 |
| MS.gene94192.t1 | MTR_7g090630 | 44.595 | 74 | 37 | 2 | 1 | 73 | 1 | 71 | 1.85e-15 | 66.6 |
| MS.gene94192.t1 | MTR_7g090630 | 44.595 | 74 | 37 | 2 | 1 | 73 | 1 | 71 | 2.02e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94192.t1 | AT2G41430 | 44.615 | 65 | 33 | 1 | 1 | 65 | 1 | 62 | 1.59e-14 | 64.7 |
| MS.gene94192.t1 | AT2G41430 | 51.786 | 56 | 24 | 1 | 1 | 56 | 1 | 53 | 3.84e-14 | 65.1 |
| MS.gene94192.t1 | AT2G41430 | 51.786 | 56 | 24 | 1 | 1 | 56 | 1 | 53 | 3.84e-14 | 65.1 |
| MS.gene94192.t1 | AT2G41430 | 51.786 | 56 | 24 | 1 | 1 | 56 | 1 | 53 | 3.84e-14 | 65.1 |
| MS.gene94192.t1 | AT2G41430 | 51.786 | 56 | 24 | 1 | 1 | 56 | 1 | 53 | 3.84e-14 | 65.1 |
| MS.gene94192.t1 | AT4G14270 | 48.485 | 66 | 26 | 2 | 12 | 75 | 10 | 69 | 4.93e-14 | 64.3 |
| MS.gene94192.t1 | AT4G14270 | 48.485 | 66 | 26 | 2 | 12 | 75 | 10 | 69 | 4.93e-14 | 64.3 |
Find 34 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCTTGGTTCCGTGATTAC+TGG | 0.164334 | 5.4:+66265240 | MS.gene94192:CDS |
| TGGAAAAGAGTTGATGAAAT+TGG | 0.190671 | 5.4:+66265568 | MS.gene94192:CDS |
| GGAAAAGAGTTGATGAAATT+GGG | 0.368922 | 5.4:+66265569 | MS.gene94192:CDS |
| ACCTCGTTCATTCCTCCCCT+TGG | 0.371694 | 5.4:+66265225 | MS.gene94192:CDS |
| TTTGATAGTTCTGAGGATCT+TGG | 0.417299 | 5.4:-66265277 | None:intergenic |
| GAGGATCTTGGAAGCGTTCA+CGG | 0.427509 | 5.4:-66265265 | None:intergenic |
| TGAAATTGGGATCGTTGAAA+TGG | 0.456391 | 5.4:+66265582 | MS.gene94192:CDS |
| GGAACGAACATCGGAGCGTT+GGG | 0.476212 | 5.4:-66265158 | None:intergenic |
| CGGAACGAACATCGGAGCGT+TGG | 0.492883 | 5.4:-66265159 | None:intergenic |
| GGGGTTCAATTTCATGAACA+TGG | 0.523834 | 5.4:-66265346 | None:intergenic |
| GCGCGTGTAGAACACGAAGA+TGG | 0.526603 | 5.4:+66265548 | MS.gene94192:intron |
| GTTGGGATTCAGCGAGGAGG+AGG | 0.529767 | 5.4:-66265141 | None:intergenic |
| TGGAGGATTTCTCCGATGAA+TGG | 0.530316 | 5.4:+66265198 | MS.gene94192:CDS |
| GAGCCTTCTCAGACATACCT+TGG | 0.535941 | 5.4:-66265632 | None:intergenic |
| GCCAGTAATCACGGAACCAA+GGG | 0.550209 | 5.4:-66265241 | None:intergenic |
| AACGAGGTTCCACCATTCAT+CGG | 0.566948 | 5.4:-66265210 | None:intergenic |
| TTGAAATGGAGAGGATCTGA+TGG | 0.578122 | 5.4:+66265596 | MS.gene94192:CDS |
| TACGCAAGCGGAACGAACAT+CGG | 0.582960 | 5.4:-66265167 | None:intergenic |
| CGTTCCGCTTGCGTATCGTA+CGG | 0.583236 | 5.4:+66265175 | MS.gene94192:CDS |
| ATGGATGGGCTGAAGTTCCA+AGG | 0.594708 | 5.4:+66265615 | MS.gene94192:CDS |
| GCTTGCGTATCGTACGGTGG+AGG | 0.600361 | 5.4:+66265181 | MS.gene94192:CDS |
| TTGGGATCGTTGAAATGGAG+AGG | 0.600872 | 5.4:+66265587 | MS.gene94192:CDS |
| AGCCAGTAATCACGGAACCA+AGG | 0.611593 | 5.4:-66265242 | None:intergenic |
| AGGATTTCTCCGATGAATGG+TGG | 0.617511 | 5.4:+66265201 | MS.gene94192:CDS |
| AATGGAGAGGATCTGATGGA+TGG | 0.618481 | 5.4:+66265600 | MS.gene94192:CDS |
| ATGGAGAGGATCTGATGGAT+GGG | 0.622633 | 5.4:+66265601 | MS.gene94192:CDS |
| GTTCACGGAGCCAGTAATCA+CGG | 0.629661 | 5.4:-66265250 | None:intergenic |
| TCCACCGTACGATACGCAAG+CGG | 0.663483 | 5.4:-66265179 | None:intergenic |
| TCCGCTTGCGTATCGTACGG+TGG | 0.666184 | 5.4:+66265178 | MS.gene94192:CDS |
| AGCGTTGGGATTCAGCGAGG+AGG | 0.680838 | 5.4:-66265144 | None:intergenic |
| GTAATCACGGAACCAAGGGG+AGG | 0.688535 | 5.4:-66265237 | None:intergenic |
| CCAGTAATCACGGAACCAAG+GGG | 0.718986 | 5.4:-66265240 | None:intergenic |
| ACCAAGGGGAGGAATGAACG+AGG | 0.740764 | 5.4:-66265226 | None:intergenic |
| CGGAGCGTTGGGATTCAGCG+AGG | 0.742236 | 5.4:-66265147 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAGTAAAATAGCTTAC+GGG | - | chr5.4:66265369-66265388 | None:intergenic | 20.0% |
| !! | CAATAAAGTAAAATAGCTTA+CGG | - | chr5.4:66265370-66265389 | None:intergenic | 20.0% |
| !! | TTTATTGTAAGAGAAAATTG+AGG | + | chr5.4:66265383-66265402 | MS.gene94192:intron | 20.0% |
| !!! | TTTTGTTACGTCAAAAAAAA+TGG | - | chr5.4:66265468-66265487 | None:intergenic | 20.0% |
| ! | ATAAAGTAAAATAGCTTACG+GGG | - | chr5.4:66265368-66265387 | None:intergenic | 25.0% |
| !!! | ATGGATCTGATGTTTTTTTT+TGG | + | chr5.4:66265526-66265545 | MS.gene94192:intron | 25.0% |
| !!! | GTTTTCTGATTTTGAAATGA+TGG | + | chr5.4:66265307-66265326 | MS.gene94192:CDS | 25.0% |
| !! | ATCGACTTTTGATGAGTTAA+TGG | + | chr5.4:66265507-66265526 | MS.gene94192:intron | 30.0% |
| !! | GACGTAACAAAAGTATGTTT+TGG | + | chr5.4:66265476-66265495 | MS.gene94192:intron | 30.0% |
| !! | GGAAAAGAGTTGATGAAATT+GGG | + | chr5.4:66265569-66265588 | MS.gene94192:CDS | 30.0% |
| !! | TGGAAAAGAGTTGATGAAAT+TGG | + | chr5.4:66265568-66265587 | MS.gene94192:CDS | 30.0% |
| TGAAATTGGGATCGTTGAAA+TGG | + | chr5.4:66265582-66265601 | MS.gene94192:CDS | 35.0% | |
| !! | GAGTCATTTTGATAGTTCTG+AGG | - | chr5.4:66265287-66265306 | None:intergenic | 35.0% |
| !! | TTTGATAGTTCTGAGGATCT+TGG | - | chr5.4:66265280-66265299 | None:intergenic | 35.0% |
| GGGGTTCAATTTCATGAACA+TGG | - | chr5.4:66265349-66265368 | None:intergenic | 40.0% | |
| TTGAAATGGAGAGGATCTGA+TGG | + | chr5.4:66265596-66265615 | MS.gene94192:CDS | 40.0% | |
| AACGAGGTTCCACCATTCAT+CGG | - | chr5.4:66265213-66265232 | None:intergenic | 45.0% | |
| AATGGAGAGGATCTGATGGA+TGG | + | chr5.4:66265600-66265619 | MS.gene94192:CDS | 45.0% | |
| AGGATTTCTCCGATGAATGG+TGG | + | chr5.4:66265201-66265220 | MS.gene94192:CDS | 45.0% | |
| TGGAGGATTTCTCCGATGAA+TGG | + | chr5.4:66265198-66265217 | MS.gene94192:CDS | 45.0% | |
| TTGGGATCGTTGAAATGGAG+AGG | + | chr5.4:66265587-66265606 | MS.gene94192:CDS | 45.0% | |
| ! | ATGGAGAGGATCTGATGGAT+GGG | + | chr5.4:66265601-66265620 | MS.gene94192:CDS | 45.0% |
| AGCCAGTAATCACGGAACCA+AGG | - | chr5.4:66265245-66265264 | None:intergenic | 50.0% | |
| CCAGTAATCACGGAACCAAG+GGG | - | chr5.4:66265243-66265262 | None:intergenic | 50.0% | |
| GCCAGTAATCACGGAACCAA+GGG | - | chr5.4:66265244-66265263 | None:intergenic | 50.0% | |
| GTTCACGGAGCCAGTAATCA+CGG | - | chr5.4:66265253-66265272 | None:intergenic | 50.0% | |
| TACGCAAGCGGAACGAACAT+CGG | - | chr5.4:66265170-66265189 | None:intergenic | 50.0% | |
| !! | ATGGATGGGCTGAAGTTCCA+AGG | + | chr5.4:66265615-66265634 | MS.gene94192:CDS | 50.0% |
| !! | GAGGATCTTGGAAGCGTTCA+CGG | - | chr5.4:66265268-66265287 | None:intergenic | 50.0% |
| ACCAAGGGGAGGAATGAACG+AGG | - | chr5.4:66265229-66265248 | None:intergenic | 55.0% | |
| ACCTCGTTCATTCCTCCCCT+TGG | + | chr5.4:66265225-66265244 | MS.gene94192:CDS | 55.0% | |
| CCCCTTGGTTCCGTGATTAC+TGG | + | chr5.4:66265240-66265259 | MS.gene94192:CDS | 55.0% | |
| CGTTCCGCTTGCGTATCGTA+CGG | + | chr5.4:66265175-66265194 | MS.gene94192:CDS | 55.0% | |
| GCGCGTGTAGAACACGAAGA+TGG | + | chr5.4:66265548-66265567 | MS.gene94192:intron | 55.0% | |
| GTAATCACGGAACCAAGGGG+AGG | - | chr5.4:66265240-66265259 | None:intergenic | 55.0% | |
| TCCACCGTACGATACGCAAG+CGG | - | chr5.4:66265182-66265201 | None:intergenic | 55.0% | |
| ! | GGAACGAACATCGGAGCGTT+GGG | - | chr5.4:66265161-66265180 | None:intergenic | 55.0% |
| CGGAACGAACATCGGAGCGT+TGG | - | chr5.4:66265162-66265181 | None:intergenic | 60.0% | |
| GCTTGCGTATCGTACGGTGG+AGG | + | chr5.4:66265181-66265200 | MS.gene94192:CDS | 60.0% | |
| GTTGGGATTCAGCGAGGAGG+AGG | - | chr5.4:66265144-66265163 | None:intergenic | 60.0% | |
| TCCGCTTGCGTATCGTACGG+TGG | + | chr5.4:66265178-66265197 | MS.gene94192:CDS | 60.0% | |
| ! | AGCGTTGGGATTCAGCGAGG+AGG | - | chr5.4:66265147-66265166 | None:intergenic | 60.0% |
| !! | CGGAGCGTTGGGATTCAGCG+AGG | - | chr5.4:66265150-66265169 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 66265113 | 66265646 | 66265113 | ID=MS.gene94192 |
| chr5.4 | mRNA | 66265113 | 66265646 | 66265113 | ID=MS.gene94192.t1;Parent=MS.gene94192 |
| chr5.4 | exon | 66265113 | 66265368 | 66265113 | ID=MS.gene94192.t1.exon1;Parent=MS.gene94192.t1 |
| chr5.4 | CDS | 66265113 | 66265368 | 66265113 | ID=cds.MS.gene94192.t1;Parent=MS.gene94192.t1 |
| chr5.4 | exon | 66265558 | 66265646 | 66265558 | ID=MS.gene94192.t1.exon2;Parent=MS.gene94192.t1 |
| chr5.4 | CDS | 66265558 | 66265646 | 66265558 | ID=cds.MS.gene94192.t1;Parent=MS.gene94192.t1 |
| Gene Sequence |
| Protein sequence |
