Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94198.t1 | XP_003616715.1 | 98.1 | 263 | 5 | 0 | 1 | 263 | 234 | 496 | 6.00E-150 | 540 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94198.t1 | G7K793 | 98.1 | 263 | 5 | 0 | 1 | 263 | 234 | 496 | 4.3e-150 | 540.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057208 | MS.gene94198 | 0.832125 | 1.13E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94198.t1 | MTR_5g083500 | 98.099 | 263 | 5 | 0 | 1 | 263 | 234 | 496 | 0.0 | 547 |
| MS.gene94198.t1 | MTR_3g071950 | 76.336 | 262 | 60 | 2 | 1 | 262 | 235 | 494 | 1.72e-147 | 422 |
| MS.gene94198.t1 | MTR_1g107165 | 32.558 | 258 | 159 | 5 | 10 | 262 | 271 | 518 | 8.46e-39 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94198.t1 | AT2G40390 | 61.977 | 263 | 100 | 0 | 1 | 263 | 234 | 496 | 2.04e-120 | 353 |
| MS.gene94198.t1 | AT5G64190 | 63.118 | 263 | 95 | 2 | 1 | 262 | 240 | 501 | 8.31e-116 | 341 |
| MS.gene94198.t1 | AT5G64190 | 63.118 | 263 | 95 | 2 | 1 | 262 | 241 | 502 | 9.05e-116 | 341 |
| MS.gene94198.t1 | AT2G15020 | 29.183 | 257 | 158 | 4 | 15 | 262 | 281 | 522 | 5.64e-32 | 123 |
Find 60 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATGCCTATTTCCATTAT+TGG | 0.166320 | 5.4:-66197335 | None:intergenic |
| AACTAGGAGGTTGGAGTTTA+GGG | 0.189509 | 5.4:+66197772 | MS.gene94198:CDS |
| AAATTTGAAGAATCGGTTTA+TGG | 0.202767 | 5.4:+66197452 | MS.gene94198:CDS |
| AGATTATGGAATGGGAAATT+AGG | 0.215093 | 5.4:+66197696 | MS.gene94198:CDS |
| AAACTAGGAGGTTGGAGTTT+AGG | 0.246270 | 5.4:+66197771 | MS.gene94198:CDS |
| GGGAAATTAGGGGTTGGATA+TGG | 0.296559 | 5.4:+66197708 | MS.gene94198:CDS |
| GAAAATCCAATAATGGAAAT+AGG | 0.304600 | 5.4:+66197329 | MS.gene94198:CDS |
| ATCCCACCTTGTCTAGTAAA+AGG | 0.308754 | 5.4:-66197608 | None:intergenic |
| CTAAGTCTTGGTTTAGAGAT+AGG | 0.319369 | 5.4:+66197567 | MS.gene94198:CDS |
| GATTATGGAATGGGAAATTA+GGG | 0.322963 | 5.4:+66197697 | MS.gene94198:CDS |
| CAGCTCCTCTTTCATTCATA+AGG | 0.338915 | 5.4:-66197211 | None:intergenic |
| TAGAATGTCCTATACTTATT+AGG | 0.345792 | 5.4:-66197743 | None:intergenic |
| AGGTGCTAGAGGGAAGATTA+TGG | 0.357263 | 5.4:+66197682 | MS.gene94198:CDS |
| ATCCTCAGAAAATCCAATAA+TGG | 0.359644 | 5.4:+66197322 | MS.gene94198:CDS |
| GTTATAGTGCAAATTTGAAT+TGG | 0.366425 | 5.4:+66197474 | MS.gene94198:CDS |
| CTTATTTGCCTAATAAGTAT+AGG | 0.370748 | 5.4:+66197735 | MS.gene94198:CDS |
| TCTCTTTGACAAAGGCTTTG+TGG | 0.374875 | 5.4:-66196548 | None:intergenic |
| CTCAAATATCAACAACTTGA+AGG | 0.385045 | 5.4:+66196510 | MS.gene94198:CDS |
| TTGGTTTCTTCATGATAGTA+TGG | 0.390260 | 5.4:+66197493 | MS.gene94198:CDS |
| GCCATGGAAATTTGAAGAAT+CGG | 0.390639 | 5.4:+66197445 | MS.gene94198:CDS |
| ACTCCAACCTCCTAGTTTCA+TGG | 0.411113 | 5.4:-66197766 | None:intergenic |
| AGTCCTATGTAGGGATTTGT+TGG | 0.445356 | 5.4:-66197383 | None:intergenic |
| AGCTCCTCTTTCATTCATAA+GGG | 0.447164 | 5.4:-66197210 | None:intergenic |
| TGGTGAACATTGACAACATA+AGG | 0.454093 | 5.4:+66196586 | MS.gene94198:CDS |
| CTATCTCTAAACCAAGACTT+AGG | 0.456609 | 5.4:-66197566 | None:intergenic |
| AATTAATTAACCCTAAGTCT+TGG | 0.472762 | 5.4:+66197555 | MS.gene94198:CDS |
| TAGAGGGAAGATTATGGAAT+GGG | 0.487526 | 5.4:+66197688 | MS.gene94198:CDS |
| TGATACATTGAGTCCTATGT+AGG | 0.489703 | 5.4:-66197393 | None:intergenic |
| AGAGAAGTGGGTCGAAATCA+TGG | 0.497766 | 5.4:+66196566 | MS.gene94198:CDS |
| ACCGATTCTTCAAATTTCCA+TGG | 0.504149 | 5.4:-66197446 | None:intergenic |
| TTTCTTCATGATAGTATGGA+TGG | 0.509936 | 5.4:+66197497 | MS.gene94198:CDS |
| ATAGGACTCAATGTATCAGC+TGG | 0.510915 | 5.4:+66197398 | MS.gene94198:CDS |
| TGGCTTTAAACTCATTGTTG+TGG | 0.514405 | 5.4:-66197426 | None:intergenic |
| GACCCACTTCTCTTTGACAA+AGG | 0.515210 | 5.4:-66196556 | None:intergenic |
| GTGTGTTGGAAAGTTGATAA+AGG | 0.526294 | 5.4:+66197662 | MS.gene94198:CDS |
| AGTTGCAATGATATTCTTGA+TGG | 0.528882 | 5.4:-66197254 | None:intergenic |
| TCCAATAATGGAAATAGGCA+TGG | 0.529685 | 5.4:+66197334 | MS.gene94198:CDS |
| GACACCCTTATGAATGAAAG+AGG | 0.534438 | 5.4:+66197206 | MS.gene94198:CDS |
| CAACAATGAGTTTAAAGCCA+TGG | 0.542148 | 5.4:+66197429 | MS.gene94198:CDS |
| TATCTCTAAACCAAGACTTA+GGG | 0.543035 | 5.4:-66197565 | None:intergenic |
| AAGACTTGATGTTGGAGTGT+TGG | 0.551465 | 5.4:-66197281 | None:intergenic |
| CTTATGAATGAAAGAGGAGC+TGG | 0.553722 | 5.4:+66197212 | MS.gene94198:CDS |
| CTACCATGAAACTAGGAGGT+TGG | 0.561866 | 5.4:+66197763 | MS.gene94198:CDS |
| ATACACTTAAGACTTGATGT+TGG | 0.563809 | 5.4:-66197289 | None:intergenic |
| TGGAATGGGAAATTAGGGGT+TGG | 0.568241 | 5.4:+66197702 | MS.gene94198:CDS |
| GGACATTCTACCATGAAACT+AGG | 0.571006 | 5.4:+66197756 | MS.gene94198:CDS |
| CAACCAACAAATCCCTACAT+AGG | 0.576027 | 5.4:+66197380 | MS.gene94198:CDS |
| AAGCCTTTGTCAAAGAGAAG+TGG | 0.583293 | 5.4:+66196553 | MS.gene94198:CDS |
| CTAGAGGGAAGATTATGGAA+TGG | 0.593772 | 5.4:+66197687 | MS.gene94198:CDS |
| GATACATTGAGTCCTATGTA+GGG | 0.594381 | 5.4:-66197392 | None:intergenic |
| GAAGTCTCTCATCAGCTGCA+TGG | 0.595852 | 5.4:-66196479 | None:intergenic |
| AAGTTGATAAAGGTGCTAGA+GGG | 0.596415 | 5.4:+66197672 | MS.gene94198:CDS |
| TAAGCTAAGGAATATTGAGA+TGG | 0.598868 | 5.4:-66197802 | None:intergenic |
| CAAGTCTTAAGTGTATCCGT+TGG | 0.601844 | 5.4:+66197296 | MS.gene94198:CDS |
| AGCCTTTGTCAAAGAGAAGT+GGG | 0.605058 | 5.4:+66196554 | MS.gene94198:CDS |
| AAAGTTGATAAAGGTGCTAG+AGG | 0.611968 | 5.4:+66197671 | MS.gene94198:CDS |
| GTTGCAATGATATTCTTGAT+GGG | 0.617241 | 5.4:-66197253 | None:intergenic |
| AATATGGAGAAAGTGTGTGT+TGG | 0.621471 | 5.4:+66197648 | MS.gene94198:CDS |
| CATTCTACCATGAAACTAGG+AGG | 0.670138 | 5.4:+66197759 | MS.gene94198:CDS |
| ATTATGGAATGGGAAATTAG+GGG | 0.711741 | 5.4:+66197698 | MS.gene94198:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAATTAATTGATTATAT+AGG | - | chr5.4:66197141-66197160 | None:intergenic | 10.0% |
| !! | ATTGAATATTTAAATTTCTT+TGG | + | chr5.4:66197154-66197173 | MS.gene94198:intron | 10.0% |
| !!! | TTAATTAATTCAAATTTTGA+AGG | - | chr5.4:66197545-66197564 | None:intergenic | 10.0% |
| !! | AATTTAATTCTATCTTGAAA+TGG | - | chr5.4:66196655-66196674 | None:intergenic | 15.0% |
| !!! | TATGTTTTTTAACATATACT+AGG | + | chr5.4:66196855-66196874 | MS.gene94198:intron | 15.0% |
| !! | AAGAATTTGTATTTGATGAA+AGG | - | chr5.4:66197047-66197066 | None:intergenic | 20.0% |
| !! | AGAATTTGTATTTGATGAAA+GGG | - | chr5.4:66197046-66197065 | None:intergenic | 20.0% |
| !!! | AAAAAACTTTTAAGGTTTGA+TGG | - | chr5.4:66196684-66196703 | None:intergenic | 20.0% |
| ! | AATTAATTAACCCTAAGTCT+TGG | + | chr5.4:66197555-66197574 | MS.gene94198:CDS | 25.0% |
| ! | ATACTCATTGTACTTCAATT+TGG | + | chr5.4:66196989-66197008 | MS.gene94198:intron | 25.0% |
| ! | ATTAAGAATCCAAATGAAAG+AGG | - | chr5.4:66196943-66196962 | None:intergenic | 25.0% |
| ! | CATCAAATACAAATTCTTTG+CGG | + | chr5.4:66197049-66197068 | MS.gene94198:intron | 25.0% |
| ! | CATTAAAAAAATCCAGTCAA+AGG | + | chr5.4:66196762-66196781 | MS.gene94198:intron | 25.0% |
| ! | CTTATTTGCCTAATAAGTAT+AGG | + | chr5.4:66197735-66197754 | MS.gene94198:CDS | 25.0% |
| ! | GTTATAGTGCAAATTTGAAT+TGG | + | chr5.4:66197474-66197493 | MS.gene94198:CDS | 25.0% |
| ! | TAGAATGTCCTATACTTATT+AGG | - | chr5.4:66197746-66197765 | None:intergenic | 25.0% |
| ! | TTAAGAATCCAAATGAAAGA+GGG | - | chr5.4:66196942-66196961 | None:intergenic | 25.0% |
| !! | AAATTTGAAGAATCGGTTTA+TGG | + | chr5.4:66197452-66197471 | MS.gene94198:CDS | 25.0% |
| !! | GAAAATCCAATAATGGAAAT+AGG | + | chr5.4:66197329-66197348 | MS.gene94198:CDS | 25.0% |
| !!! | CTTTGACTGGATTTTTTTAA+TGG | - | chr5.4:66196764-66196783 | None:intergenic | 25.0% |
| !!! | TGGATTTTTTTAATGGCAAA+TGG | - | chr5.4:66196757-66196776 | None:intergenic | 25.0% |
| !!! | TTAGTTTTTTTTCCTTTGAC+TGG | - | chr5.4:66196777-66196796 | None:intergenic | 25.0% |
| AGATTATGGAATGGGAAATT+AGG | + | chr5.4:66197696-66197715 | MS.gene94198:CDS | 30.0% | |
| AGTTGCAATGATATTCTTGA+TGG | - | chr5.4:66197257-66197276 | None:intergenic | 30.0% | |
| ATACACTTAAGACTTGATGT+TGG | - | chr5.4:66197292-66197311 | None:intergenic | 30.0% | |
| ATCCTCAGAAAATCCAATAA+TGG | + | chr5.4:66197322-66197341 | MS.gene94198:CDS | 30.0% | |
| ATTATGGAATGGGAAATTAG+GGG | + | chr5.4:66197698-66197717 | MS.gene94198:CDS | 30.0% | |
| CTCAAATATCAACAACTTGA+AGG | + | chr5.4:66196510-66196529 | MS.gene94198:CDS | 30.0% | |
| GATTATGGAATGGGAAATTA+GGG | + | chr5.4:66197697-66197716 | MS.gene94198:CDS | 30.0% | |
| GTTGCAATGATATTCTTGAT+GGG | - | chr5.4:66197256-66197275 | None:intergenic | 30.0% | |
| TATCTCTAAACCAAGACTTA+GGG | - | chr5.4:66197568-66197587 | None:intergenic | 30.0% | |
| TATGTCTGTGTATGTATTAC+AGG | + | chr5.4:66196905-66196924 | MS.gene94198:intron | 30.0% | |
| TTGGTTTCTTCATGATAGTA+TGG | + | chr5.4:66197493-66197512 | MS.gene94198:CDS | 30.0% | |
| TTTCTTCATGATAGTATGGA+TGG | + | chr5.4:66197497-66197516 | MS.gene94198:CDS | 30.0% | |
| ! | TTCCATTATTGGATTTTCTG+AGG | - | chr5.4:66197327-66197346 | None:intergenic | 30.0% |
| ! | TTTTCAAACCCTCTTTCATT+TGG | + | chr5.4:66196931-66196950 | MS.gene94198:intron | 30.0% |
| !! | ATTTTCTGAGGATTTTCCAA+CGG | - | chr5.4:66197315-66197334 | None:intergenic | 30.0% |
| !! | ATTTTTGCTGGTGATGAATA+TGG | + | chr5.4:66197632-66197651 | MS.gene94198:CDS | 30.0% |
| AGAAAAAGCAACGATTCGAT+TGG | - | chr5.4:66196882-66196901 | None:intergenic | 35.0% | |
| AGCTCCTCTTTCATTCATAA+GGG | - | chr5.4:66197213-66197232 | None:intergenic | 35.0% | |
| CTAAGTCTTGGTTTAGAGAT+AGG | + | chr5.4:66197567-66197586 | MS.gene94198:CDS | 35.0% | |
| CTATCTCTAAACCAAGACTT+AGG | - | chr5.4:66197569-66197588 | None:intergenic | 35.0% | |
| GATACATTGAGTCCTATGTA+GGG | - | chr5.4:66197395-66197414 | None:intergenic | 35.0% | |
| GCCATGGAAATTTGAAGAAT+CGG | + | chr5.4:66197445-66197464 | MS.gene94198:CDS | 35.0% | |
| TAGAGGGAAGATTATGGAAT+GGG | + | chr5.4:66197688-66197707 | MS.gene94198:CDS | 35.0% | |
| TCCATGCCTATTTCCATTAT+TGG | - | chr5.4:66197338-66197357 | None:intergenic | 35.0% | |
| TGATACATTGAGTCCTATGT+AGG | - | chr5.4:66197396-66197415 | None:intergenic | 35.0% | |
| TGGTGAACATTGACAACATA+AGG | + | chr5.4:66196586-66196605 | MS.gene94198:CDS | 35.0% | |
| ! | AATATGGAGAAAGTGTGTGT+TGG | + | chr5.4:66197648-66197667 | MS.gene94198:CDS | 35.0% |
| ! | ACCGATTCTTCAAATTTCCA+TGG | - | chr5.4:66197449-66197468 | None:intergenic | 35.0% |
| ! | CAACAATGAGTTTAAAGCCA+TGG | + | chr5.4:66197429-66197448 | MS.gene94198:CDS | 35.0% |
| ! | TATCGTCCTTTTACTAGACA+AGG | + | chr5.4:66197602-66197621 | MS.gene94198:CDS | 35.0% |
| ! | TCCAATAATGGAAATAGGCA+TGG | + | chr5.4:66197334-66197353 | MS.gene94198:CDS | 35.0% |
| !! | AAAGTTGATAAAGGTGCTAG+AGG | + | chr5.4:66197671-66197690 | MS.gene94198:CDS | 35.0% |
| !! | AAGTTGATAAAGGTGCTAGA+GGG | + | chr5.4:66197672-66197691 | MS.gene94198:CDS | 35.0% |
| !! | GTGTGTTGGAAAGTTGATAA+AGG | + | chr5.4:66197662-66197681 | MS.gene94198:CDS | 35.0% |
| !! | TGGCTTTAAACTCATTGTTG+TGG | - | chr5.4:66197429-66197448 | None:intergenic | 35.0% |
| AAACTAGGAGGTTGGAGTTT+AGG | + | chr5.4:66197771-66197790 | MS.gene94198:CDS | 40.0% | |
| AACTAGGAGGTTGGAGTTTA+GGG | + | chr5.4:66197772-66197791 | MS.gene94198:CDS | 40.0% | |
| AAGACTTGATGTTGGAGTGT+TGG | - | chr5.4:66197284-66197303 | None:intergenic | 40.0% | |
| AAGCCTTTGTCAAAGAGAAG+TGG | + | chr5.4:66196553-66196572 | MS.gene94198:CDS | 40.0% | |
| AGCCTTTGTCAAAGAGAAGT+GGG | + | chr5.4:66196554-66196573 | MS.gene94198:CDS | 40.0% | |
| AGTCCTATGTAGGGATTTGT+TGG | - | chr5.4:66197386-66197405 | None:intergenic | 40.0% | |
| ATAGGACTCAATGTATCAGC+TGG | + | chr5.4:66197398-66197417 | MS.gene94198:CDS | 40.0% | |
| ATCCCACCTTGTCTAGTAAA+AGG | - | chr5.4:66197611-66197630 | None:intergenic | 40.0% | |
| CAACCAACAAATCCCTACAT+AGG | + | chr5.4:66197380-66197399 | MS.gene94198:CDS | 40.0% | |
| CAAGTCTTAAGTGTATCCGT+TGG | + | chr5.4:66197296-66197315 | MS.gene94198:CDS | 40.0% | |
| CAGCTCCTCTTTCATTCATA+AGG | - | chr5.4:66197214-66197233 | None:intergenic | 40.0% | |
| CATTCTACCATGAAACTAGG+AGG | + | chr5.4:66197759-66197778 | MS.gene94198:CDS | 40.0% | |
| CTAGAGGGAAGATTATGGAA+TGG | + | chr5.4:66197687-66197706 | MS.gene94198:CDS | 40.0% | |
| CTTATGAATGAAAGAGGAGC+TGG | + | chr5.4:66197212-66197231 | MS.gene94198:CDS | 40.0% | |
| GACACCCTTATGAATGAAAG+AGG | + | chr5.4:66197206-66197225 | MS.gene94198:CDS | 40.0% | |
| GGACATTCTACCATGAAACT+AGG | + | chr5.4:66197756-66197775 | MS.gene94198:CDS | 40.0% | |
| GTCTGTGTATGTATTACAGG+TGG | + | chr5.4:66196908-66196927 | MS.gene94198:intron | 40.0% | |
| TCTCTTTGACAAAGGCTTTG+TGG | - | chr5.4:66196551-66196570 | None:intergenic | 40.0% | |
| ! | GTCCTTTTACTAGACAAGGT+GGG | + | chr5.4:66197606-66197625 | MS.gene94198:CDS | 40.0% |
| !!! | CAAGGTGGGATTATTTTTGC+TGG | + | chr5.4:66197620-66197639 | MS.gene94198:CDS | 40.0% |
| ACTCCAACCTCCTAGTTTCA+TGG | - | chr5.4:66197769-66197788 | None:intergenic | 45.0% | |
| AGAGAAGTGGGTCGAAATCA+TGG | + | chr5.4:66196566-66196585 | MS.gene94198:CDS | 45.0% | |
| AGGTGCTAGAGGGAAGATTA+TGG | + | chr5.4:66197682-66197701 | MS.gene94198:CDS | 45.0% | |
| CTACCATGAAACTAGGAGGT+TGG | + | chr5.4:66197763-66197782 | MS.gene94198:CDS | 45.0% | |
| GACCCACTTCTCTTTGACAA+AGG | - | chr5.4:66196559-66196578 | None:intergenic | 45.0% | |
| GGGAAATTAGGGGTTGGATA+TGG | + | chr5.4:66197708-66197727 | MS.gene94198:CDS | 45.0% | |
| TGGAATGGGAAATTAGGGGT+TGG | + | chr5.4:66197702-66197721 | MS.gene94198:CDS | 45.0% | |
| ! | CGTCCTTTTACTAGACAAGG+TGG | + | chr5.4:66197605-66197624 | MS.gene94198:CDS | 45.0% |
| !!! | AAAAAAAAAAAAAACTTTTA+AGG | - | chr5.4:66196692-66196711 | None:intergenic | 5.0% |
| !!! | TTTAATTTATTTTTTGTTTT+AGG | + | chr5.4:66197101-66197120 | MS.gene94198:intron | 5.0% |
| GAAGTCTCTCATCAGCTGCA+TGG | - | chr5.4:66196482-66196501 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 66196456 | 66197820 | 66196456 | ID=MS.gene94198 |
| chr5.4 | mRNA | 66196456 | 66197820 | 66196456 | ID=MS.gene94198.t1;Parent=MS.gene94198 |
| chr5.4 | exon | 66196456 | 66196607 | 66196456 | ID=MS.gene94198.t1.exon1;Parent=MS.gene94198.t1 |
| chr5.4 | CDS | 66196456 | 66196607 | 66196456 | ID=cds.MS.gene94198.t1;Parent=MS.gene94198.t1 |
| chr5.4 | exon | 66197181 | 66197820 | 66197181 | ID=MS.gene94198.t1.exon2;Parent=MS.gene94198.t1 |
| chr5.4 | CDS | 66197181 | 66197820 | 66197181 | ID=cds.MS.gene94198.t1;Parent=MS.gene94198.t1 |
| Gene Sequence |
| Protein sequence |
