AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94545

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94545.t1 MTR_8g106980 87.816 435 34 7 1 429 1 422 0.0 623
MS.gene94545.t1 MTR_6g079520 33.735 332 124 10 127 389 215 519 4.18e-27 114
MS.gene94545.t1 MTR_6g079520 71.233 73 19 1 34 104 42 114 8.65e-24 103
MS.gene94545.t1 MTR_5g078330 38.182 165 64 5 35 188 13 150 6.65e-19 85.1
MS.gene94545.t1 MTR_3g065570 35.057 174 100 6 35 204 14 178 2.39e-13 68.6
MS.gene94545.t1 MTR_5g021730 44.156 77 43 0 31 107 32 108 2.32e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94545.t1 AT3G18035 34.500 400 176 18 29 361 60 440 1.20e-35 137
MS.gene94545.t1 AT1G48620 33.666 401 170 18 37 359 78 460 9.47e-33 129
MS.gene94545.t1 AT3G18035 35.350 314 130 14 29 279 60 363 3.22e-26 109
MS.gene94545.t1 AT1G14900 38.889 144 52 4 35 172 24 137 1.75e-16 78.2

Find 168 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACGACCTCTTTGGGTTAA+AGG 0.177310 4.1:+472042 None:intergenic
ATTTGGTATGGGGTTGGGCT+TGG 0.190257 4.1:+472005 None:intergenic
TGGCCTTCCGGAGGGATTTA+TGG 0.225963 4.1:+471615 None:intergenic
CATCTCTTAATGGTGCTTTA+AGG 0.229813 4.1:+470756 None:intergenic
ATACTTCTTGGGGCGGCCTC+TGG 0.231487 4.1:+471702 None:intergenic
TCTGTGCCTGTGGCACTTGC+TGG 0.231987 4.1:+470576 None:intergenic
GATACATTGGCTTCATCTTC+TGG 0.253002 4.1:+471742 None:intergenic
TGGGCATTTGGTATGGGGTT+GGG 0.253909 4.1:+472000 None:intergenic
CCCACCACGGCAAGCTTCTT+AGG 0.260962 4.1:+471643 None:intergenic
TTTGGTATGGGGTTGGGCTT+GGG 0.268353 4.1:+472006 None:intergenic
ATGGGGTTGGGCTTGGGCTT+AGG 0.277165 4.1:+472012 None:intergenic
GTTAGAAATGCTGGCAAGTT+TGG 0.278967 4.1:-470780 MS.gene94545:CDS
GAGCCCATTGGACACAGATT+TGG 0.290684 4.1:+471867 None:intergenic
TGTTGCAGCGGTTGTGATTC+CGG 0.297851 4.1:+470710 None:intergenic
CACCGCATCTGCATACTTCT+TGG 0.310561 4.1:+471690 None:intergenic
TGAAGTGTTTCATCTCTTAA+TGG 0.311071 4.1:+470746 None:intergenic
CAGCGCCGGTGGCACTGGTT+GGG 0.311789 4.1:+470619 None:intergenic
CCTGTTGCTGCAATGGCATC+TGG 0.319472 4.1:+470534 None:intergenic
TGGGCGGCCAATTGGCCTTC+CGG 0.321471 4.1:+471603 None:intergenic
TACTTCTTGGGGCGGCCTCT+GGG 0.324206 4.1:+471703 None:intergenic
AGCCCATTGGACACAGATTT+GGG 0.328566 4.1:+471868 None:intergenic
TGCTGCACCTGTTGCTGCAA+TGG 0.336500 4.1:+470527 None:intergenic
GGCCTTCCGGAGGGATTTAT+GGG 0.345829 4.1:+471616 None:intergenic
AAGGGAACGGTGGTGGGTAG+AGG 0.347976 4.1:+472381 None:intergenic
ACGAAAACGGGCTGGGCATT+TGG 0.348576 4.1:+471988 None:intergenic
CATGTAAATCTTCCTAGCTT+GGG 0.356230 4.1:+470480 None:intergenic
CTATTGAGAGTGGGAGGTGG+AGG 0.361661 4.1:+472099 None:intergenic
ATCACCTTTGGGCGGCCAAT+TGG 0.362515 4.1:+471595 None:intergenic
GGAACGGTGGTGGGTAGAGG+TGG 0.367498 4.1:+472384 None:intergenic
TTGCCAGCATTTCTAACTCT+TGG 0.371407 4.1:+470786 None:intergenic
CTGGGCATTTGGTATGGGGT+TGG 0.372983 4.1:+471999 None:intergenic
ACGGTGGTGGGTAGAGGTGG+AGG 0.374446 4.1:+472387 None:intergenic
ACCGCATCTGCATACTTCTT+GGG 0.385512 4.1:+471691 None:intergenic
AGCGCCGGTGGCACTGGTTG+GGG 0.396155 4.1:+470620 None:intergenic
AGGACGACCACGACCTCTTT+GGG 0.396456 4.1:+472035 None:intergenic
GAGGACGACCACGACCTCTT+TGG 0.397582 4.1:+472034 None:intergenic
ACTTCAGCCACAGCAATTTC+CGG 0.398242 4.1:-470729 MS.gene94545:CDS
GTGGTGGGTAGAGGTGGAGG+AGG 0.401260 4.1:+472390 None:intergenic
GCAGCGCCGGTGGCACTGGT+TGG 0.403759 4.1:+470618 None:intergenic
CTGTGCCTGTGGCACTTGCT+GGG 0.404679 4.1:+470577 None:intergenic
GGAGGTGGAGGGAGTGAGGA+AGG 0.407024 4.1:+472111 None:intergenic
AGTGGGAGGTGGAGGGAGTG+AGG 0.410669 4.1:+472107 None:intergenic
AACGGGCTGGGCATTTGGTA+TGG 0.411734 4.1:+471993 None:intergenic
GCAGCGACTGTGACACTGGC+TGG 0.411975 4.1:+470660 None:intergenic
CCAGATGCCATTGCAGCAAC+AGG 0.415754 4.1:-470534 MS.gene94545:CDS
GGATCAAGCAAAAGGGCAAT+AGG 0.418614 4.1:-472258 MS.gene94545:CDS
ACGGGCTGGGCATTTGGTAT+GGG 0.421048 4.1:+471994 None:intergenic
TTTGCAGCAATCAAAGGTTA+AGG 0.422418 4.1:-470873 MS.gene94545:intron
AGAGGGCGTGGACGTCCTCC+TGG 0.422567 4.1:-471919 MS.gene94545:CDS
AAAATAAAATAGGGAGCAAA+AGG 0.431280 4.1:-471028 MS.gene94545:intron
ACAGGCTCCACAGCAGTTGT+AGG 0.440856 4.1:+471946 None:intergenic
CCATGTAAATCTTCCTAGCT+TGG 0.443512 4.1:+470479 None:intergenic
ATTGCTGCAACTGCAGTGGC+TGG 0.445073 4.1:+470809 None:intergenic
CACGTGGTGGTGAGTGAAGT+TGG 0.448823 4.1:+472344 None:intergenic
AGAAGCTTGCCGTGGTGGGC+AGG 0.453200 4.1:-471639 MS.gene94545:CDS
GAGGGTGAAGGGAACGGTGG+TGG 0.454084 4.1:+472374 None:intergenic
AACAAGGTGCTGTGTTCTGC+TGG 0.458813 4.1:+472201 None:intergenic
GCAAAAGGTATGACAGTTGT+TGG 0.460250 4.1:+471799 None:intergenic
TGCTGAGATGGCAGCGCCGG+TGG 0.462473 4.1:+470608 None:intergenic
AGAAAGATGGATCAAGCAAA+AGG 0.462833 4.1:-472266 MS.gene94545:CDS
GTTGGGTTGTTCGAGGGTGA+AGG 0.465533 4.1:+472362 None:intergenic
CCGGTGGCACTGGTTGGGGA+TGG 0.467209 4.1:+470624 None:intergenic
AATCCCTCCGGAAGGCCAAT+TGG 0.470273 4.1:-471610 MS.gene94545:CDS
ACAGCAGTTGTAGGAGGAGG+AGG 0.471817 4.1:+471955 None:intergenic
GGACGACCCCGCCTTGTCGC+TGG 0.472354 4.1:+471664 None:intergenic
GGGTTGGGCTTGGGCTTAGG+AGG 0.475288 4.1:+472015 None:intergenic
GATGGCAGCGCCGGTGGCAC+TGG 0.477721 4.1:+470614 None:intergenic
TTGTTGGTTGCGACTGCGAT+TGG 0.477868 4.1:+471815 None:intergenic
ACAACAGAAGGAAAGGGAAC+AGG 0.483163 4.1:+471766 None:intergenic
TGGTGGGTAGAGGTGGAGGA+GGG 0.485584 4.1:+472391 None:intergenic
TTCACTCCTTTAACCCAAAG+AGG 0.486192 4.1:-472048 MS.gene94545:CDS
GGCCCAAATCTGTGTCCAAT+GGG 0.489592 4.1:-471870 MS.gene94545:CDS
CTCAACCACTTGAAATCTGC+TGG 0.490237 4.1:-472168 MS.gene94545:CDS
CATCTCAGCGTAAGGAGGAT+GGG 0.490876 4.1:+472317 None:intergenic
AATCCAAGAGTTAGAAATGC+TGG 0.492647 4.1:-470789 MS.gene94545:CDS
GCGGCCAATTGGCCTTCCGG+AGG 0.493639 4.1:+471606 None:intergenic
GGTGGTTCCAGCGACAAGGC+GGG 0.496767 4.1:-471671 MS.gene94545:CDS
AGGTGGAGGAGGGAGATCAA+AGG 0.498529 4.1:+472401 None:intergenic
CTTTGGGTTAAAGGAGTGAA+AGG 0.498701 4.1:+472051 None:intergenic
TCCTAAGAAGCTTGCCGTGG+TGG 0.501049 4.1:-471644 MS.gene94545:CDS
GAGTGAAGTTGGGTTGTTCG+AGG 0.501548 4.1:+472355 None:intergenic
AGGACGTCGTTTGAGCCCAT+TGG 0.502539 4.1:+471855 None:intergenic
CGGCCAATTGGCCTTCCGGA+GGG 0.504424 4.1:+471607 None:intergenic
TAGCTTGGGGCTAAAGTATG+TGG 0.505368 4.1:+470494 None:intergenic
TGTTGGTTGCGACTGCGATT+GGG 0.505909 4.1:+471816 None:intergenic
TGGGGATGCAGTTGCTGAGA+TGG 0.508395 4.1:+470596 None:intergenic
CGAGAGAAACGAAAACGGGC+TGG 0.514942 4.1:+471980 None:intergenic
CCATCCCCAACCAGTGCCAC+CGG 0.514985 4.1:-470624 MS.gene94545:CDS
CGGGCTGGGCATTTGGTATG+GGG 0.518221 4.1:+471995 None:intergenic
GATGGCAGCGACTGTGACAC+TGG 0.521680 4.1:+470656 None:intergenic
TTCTGGAACAACAGAAGGAA+AGG 0.523237 4.1:+471759 None:intergenic
ACAGGAGCAGCAGCAGCAAA+AGG 0.523324 4.1:+471784 None:intergenic
TGGGAATGCAGTTGCTGAGA+TGG 0.525277 4.1:+470680 None:intergenic
AGGGTGAAGGGAACGGTGGT+GGG 0.527527 4.1:+472375 None:intergenic
TCCACAGCAGTTGTAGGAGG+AGG 0.528426 4.1:+471952 None:intergenic
TTGTTCGAGGGTGAAGGGAA+CGG 0.529100 4.1:+472368 None:intergenic
TCATCTCAGCGTAAGGAGGA+TGG 0.530449 4.1:+472316 None:intergenic
AATGGGCTCAAACGACGTCC+TGG 0.531113 4.1:-471853 MS.gene94545:CDS
GCATCCCCAGCAAGTGCCAC+AGG 0.532601 4.1:-470582 MS.gene94545:CDS
TCATCTTCTGGAACAACAGA+AGG 0.535649 4.1:+471754 None:intergenic
TGGGGATGGAGTTGCAGAGA+TGG 0.537472 4.1:+470638 None:intergenic
CCTAAGAAGCTTGCCGTGGT+GGG 0.538381 4.1:-471643 MS.gene94545:CDS
AGGGTCGAGAGAAACGAAAA+CGG 0.539218 4.1:+471975 None:intergenic
TCAGATCTAACTCGATAAAG+AGG 0.543241 4.1:-471891 MS.gene94545:CDS
ACGTGGTGGTGAGTGAAGTT+GGG 0.543540 4.1:+472345 None:intergenic
GAGAGAAACGAAAACGGGCT+GGG 0.545382 4.1:+471981 None:intergenic
GGCAGGCCCATAAATCCCTC+CGG 0.545558 4.1:-471622 MS.gene94545:CDS
GAAGCACTGAAAGAGAAAGA+TGG 0.550195 4.1:-472279 MS.gene94545:CDS
AGGCCCAAATCTGTGTCCAA+TGG 0.550214 4.1:-471871 MS.gene94545:CDS
ATCAAAGGAAGTTGAGTCCA+TGG 0.551511 4.1:+472416 None:intergenic
TATTGAGAGTGGGAGGTGGA+GGG 0.551861 4.1:+472100 None:intergenic
GGGTCGAGAGAAACGAAAAC+GGG 0.555283 4.1:+471976 None:intergenic
GAAAGATGGATCAAGCAAAA+GGG 0.555450 4.1:-472265 MS.gene94545:CDS
GAGCCTGTTAAAAGAGGGCG+TGG 0.555621 4.1:-471931 MS.gene94545:CDS
GCTCACTTTGCAGCAATCAA+AGG 0.556497 4.1:-470879 MS.gene94545:intron
TCCTCCTCCTACAACTGCTG+TGG 0.559098 4.1:-471953 MS.gene94545:CDS
GAGGGCGTGGACGTCCTCCT+GGG 0.559128 4.1:-471918 MS.gene94545:CDS
TTGGGTTGTTCGAGGGTGAA+GGG 0.559588 4.1:+472363 None:intergenic
AGTGAAGTTGGGTTGTTCGA+GGG 0.560599 4.1:+472356 None:intergenic
TGTGCCTGTGGCACTTGCTG+GGG 0.560848 4.1:+470578 None:intergenic
ATCTCAGCGTAAGGAGGATG+GGG 0.561129 4.1:+472318 None:intergenic
AGTTGCTGAGATGGCAGCGC+CGG 0.561561 4.1:+470605 None:intergenic
TTGTATATCATCTCAGCGTA+AGG 0.563264 4.1:+472309 None:intergenic
GGCTCCACAGCAGTTGTAGG+AGG 0.564347 4.1:+471949 None:intergenic
TTGGATTGCTGCAACTGCAG+TGG 0.565398 4.1:+470805 None:intergenic
TTGTCAATGAAGATCTAAGA+AGG 0.565742 4.1:-470997 MS.gene94545:CDS
CAGCGACTGTGACACTGGCT+GGG 0.567986 4.1:+470661 None:intergenic
AAGGCCAATTGGCCGCCCAA+AGG 0.570612 4.1:-471599 MS.gene94545:intron
GCTGTGGAGCCTGTTAAAAG+AGG 0.572101 4.1:-471937 MS.gene94545:CDS
GTGGTCAGATCTATTGAGAG+TGG 0.574173 4.1:+472089 None:intergenic
TTTGGGTTAAAGGAGTGAAA+GGG 0.574578 4.1:+472052 None:intergenic
ATGTATCTGTTAGATCTACT+AGG 0.575325 4.1:-471726 MS.gene94545:CDS
GTAGACCAGCAGATTTCAAG+TGG 0.580659 4.1:+472163 None:intergenic
TCTGGAACAACAGAAGGAAA+GGG 0.582719 4.1:+471760 None:intergenic
CCCCAAGAAGTATGCAGATG+CGG 0.582736 4.1:-471692 MS.gene94545:CDS
GATCTATTGAGAGTGGGAGG+TGG 0.583566 4.1:+472096 None:intergenic
GAGGATGGGGTGCCACGTGG+TGG 0.584493 4.1:+472331 None:intergenic
AGTAGATCTAACAGATACAT+TGG 0.584810 4.1:+471729 None:intergenic
ACATACTTTAGCCCCAAGCT+AGG 0.588129 4.1:-470492 None:intergenic
CCGCATCTGCATACTTCTTG+GGG 0.591852 4.1:+471692 None:intergenic
CCTTTAACCCAAAGAGGTCG+TGG 0.592542 4.1:-472042 MS.gene94545:CDS
GAGTTAGATCTGAAAGTCCC+AGG 0.592545 4.1:+471901 None:intergenic
CTGTGGAGCCTGTTAAAAGA+GGG 0.595055 4.1:-471936 MS.gene94545:CDS
GGCCCATAAATCCCTCCGGA+AGG 0.596807 4.1:-471618 MS.gene94545:CDS
TGTTCATAATTCTGTGCCTG+TGG 0.597800 4.1:+470566 None:intergenic
ATGTAAATCTTCCTAGCTTG+GGG 0.601628 4.1:+470481 None:intergenic
TGGTCAGATCTATTGAGAGT+GGG 0.603697 4.1:+472090 None:intergenic
CGGTGGTTCCAGCGACAAGG+CGG 0.609470 4.1:-471672 MS.gene94545:CDS
ATTTATGGGCCTGCCCACCA+CGG 0.610897 4.1:+471630 None:intergenic
GGACTTTGATGATCAAAGAG+AGG 0.613003 4.1:+470830 None:intergenic
ATGCGGTGGTTCCAGCGACA+AGG 0.614719 4.1:-471675 MS.gene94545:CDS
AAGGAGGATGGGGTGCCACG+TGG 0.617913 4.1:+472328 None:intergenic
GTGGTTCCAGCGACAAGGCG+GGG 0.619166 4.1:-471670 MS.gene94545:CDS
TATATCATCTCAGCGTAAGG+AGG 0.625776 4.1:+472312 None:intergenic
GATGGCAGCTCTTGTTGCAG+CGG 0.632179 4.1:+470698 None:intergenic
CAGCAGTTGTAGGAGGAGGA+GGG 0.641753 4.1:+471956 None:intergenic
TCGTCCTAAGAAGCTTGCCG+TGG 0.649764 4.1:-471647 MS.gene94545:CDS
ACATTAGGATCACCTTTGGG+CGG 0.663242 4.1:+471587 None:intergenic
CAAGAAGTATGCAGATGCGG+TGG 0.663985 4.1:-471689 MS.gene94545:CDS
TGTGATTCCGGAAATTGCTG+TGG 0.664513 4.1:+470722 None:intergenic
TTCGAGGGTGAAGGGAACGG+TGG 0.666611 4.1:+472371 None:intergenic
TCAGATCTATTGAGAGTGGG+AGG 0.671854 4.1:+472093 None:intergenic
GTTGAGATGTTGTGTTAACA+AGG 0.674763 4.1:+472185 None:intergenic
CATCTGCATACTTCTTGGGG+CGG 0.687425 4.1:+471695 None:intergenic
CAACTTCACTCACCACCACG+TGG 0.718293 4.1:-472343 MS.gene94545:CDS
AAGGGAGATGAGCTTCAGAG+TGG 0.733637 4.1:+472070 None:intergenic
GTTAGATCTACTAGGCCCAG+AGG 0.757336 4.1:-471718 MS.gene94545:CDS
TTAGATCTGAAAGTCCCAGG+AGG 0.781122 4.1:+471904 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATAGTTAATCATTTA+GGG + chr4.1:471464-471483 None:intergenic 10.0%
!!! AAAAAATCTTTAATTTATAC+AGG + chr4.1:471840-471859 None:intergenic 10.0%
!!! CATTTTCTATAAAATAAAAT+AGG - chr4.1:471869-471888 MS.gene94545:CDS 10.0%
!! CATTAAATAGTTAATCATTT+AGG + chr4.1:471465-471484 None:intergenic 15.0%
!! AACAATAAAAACTCAACATT+AGG + chr4.1:471338-471357 None:intergenic 20.0%
!! AGTTGAATTTATCTTTCATT+TGG - chr4.1:471951-471970 MS.gene94545:CDS 20.0%
!!! TAAATTCAATTTGTTGTCTT+TGG - chr4.1:471727-471746 MS.gene94545:CDS 20.0%
! AAAATAAAATAGGGAGCAAA+AGG - chr4.1:471879-471898 MS.gene94545:CDS 25.0%
! AAACACACATAAATAGATGA+AGG + chr4.1:471362-471381 None:intergenic 25.0%
! GTAATAAACAATAAAGCAGA+TGG - chr4.1:471578-471597 MS.gene94545:intron 25.0%
!!! TAATTTGACTTGCCTTATTT+TGG - chr4.1:471401-471420 MS.gene94545:intron 25.0%
AGTAATTAAGCAAGTAGAGA+GGG - chr4.1:471648-471667 MS.gene94545:CDS 30.0%
AGTAGATCTAACAGATACAT+TGG + chr4.1:471181-471200 None:intergenic 30.0%
ATGTATCTGTTAGATCTACT+AGG - chr4.1:471181-471200 MS.gene94545:intron 30.0%
CACATTTATCTTGAAAACGA+AGG - chr4.1:471528-471547 MS.gene94545:intron 30.0%
TAGTAATTAAGCAAGTAGAG+AGG - chr4.1:471647-471666 MS.gene94545:CDS 30.0%
TCTCATACTAGACCAAAATA+AGG + chr4.1:471416-471435 None:intergenic 30.0%
TTGGTCTAGTATGAGATTAT+GGG - chr4.1:471420-471439 MS.gene94545:intron 30.0%
TTGTCAATGAAGATCTAAGA+AGG - chr4.1:471910-471929 MS.gene94545:CDS 30.0%
TTTGGTCTAGTATGAGATTA+TGG - chr4.1:471419-471438 MS.gene94545:intron 30.0%
TTTGTTTCCAAAAGTCAATG+TGG + chr4.1:471507-471526 None:intergenic 30.0%
! TGAAGTGTTTCATCTCTTAA+TGG + chr4.1:472164-472183 None:intergenic 30.0%
!! TGTTGTCTTTGGAACATATT+TGG - chr4.1:471738-471757 MS.gene94545:CDS 30.0%
AATCCAAGAGTTAGAAATGC+TGG - chr4.1:472118-472137 MS.gene94545:CDS 35.0%
AGAAAGATGGATCAAGCAAA+AGG - chr4.1:470641-470660 MS.gene94545:CDS 35.0%
CATCTCTTAATGGTGCTTTA+AGG + chr4.1:472154-472173 None:intergenic 35.0%
GAAAGATGGATCAAGCAAAA+GGG - chr4.1:470642-470661 MS.gene94545:CDS 35.0%
GTTGAGATGTTGTGTTAACA+AGG + chr4.1:470725-470744 None:intergenic 35.0%
TCAACATTAGGATCACCTTT+GGG + chr4.1:471326-471345 None:intergenic 35.0%
TCAGATCTAACTCGATAAAG+AGG - chr4.1:471016-471035 MS.gene94545:CDS 35.0%
TTGTATATCATCTCAGCGTA+AGG + chr4.1:470601-470620 None:intergenic 35.0%
TTTGCAGCAATCAAAGGTTA+AGG - chr4.1:472034-472053 MS.gene94545:CDS 35.0%
! AAACGAAGGCATGTCATATA+TGG - chr4.1:471542-471561 MS.gene94545:intron 35.0%
! TTTGGGTTAAAGGAGTGAAA+GGG + chr4.1:470858-470877 None:intergenic 35.0%
!!! AGCTTATCCACATTGACTTT+TGG - chr4.1:471497-471516 MS.gene94545:intron 35.0%
CTCAACATTAGGATCACCTT+TGG + chr4.1:471327-471346 None:intergenic 40.0%
GCAAAGTGAGCAAACAATAG+AGG + chr4.1:472019-472038 None:intergenic 40.0%
GTTAACATTCTCGAGCTATG+TGG - chr4.1:471767-471786 MS.gene94545:CDS 40.0%
GTTAGAAATGCTGGCAAGTT+TGG - chr4.1:472127-472146 MS.gene94545:CDS 40.0%
TATATCATCTCAGCGTAAGG+AGG + chr4.1:470598-470617 None:intergenic 40.0%
TGGTCAGATCTATTGAGAGT+GGG + chr4.1:470820-470839 None:intergenic 40.0%
TGTTCATAATTCTGTGCCTG+TGG + chr4.1:472344-472363 None:intergenic 40.0%
TTCACTCCTTTAACCCAAAG+AGG - chr4.1:470859-470878 MS.gene94545:CDS 40.0%
TTGCCAGCATTTCTAACTCT+TGG + chr4.1:472124-472143 None:intergenic 40.0%
! CTTTGGGTTAAAGGAGTGAA+AGG + chr4.1:470859-470878 None:intergenic 40.0%
! GCAAAAGGTATGACAGTTGT+TGG + chr4.1:471111-471130 None:intergenic 40.0%
! GGACTTTGATGATCAAAGAG+AGG + chr4.1:472080-472099 None:intergenic 40.0%
! TCTGGAACAACAGAAGGAAA+GGG + chr4.1:471150-471169 None:intergenic 40.0%
!! GAAGCACTGAAAGAGAAAGA+TGG - chr4.1:470628-470647 MS.gene94545:CDS 40.0%
!! GATACATTGGCTTCATCTTC+TGG + chr4.1:471168-471187 None:intergenic 40.0%
!! TCATCTTCTGGAACAACAGA+AGG + chr4.1:471156-471175 None:intergenic 40.0%
!! TTCTGGAACAACAGAAGGAA+AGG + chr4.1:471151-471170 None:intergenic 40.0%
ACAACAGAAGGAAAGGGAAC+AGG + chr4.1:471144-471163 None:intergenic 45.0%
ACATTAGGATCACCTTTGGG+CGG + chr4.1:471323-471342 None:intergenic 45.0%
ACCGCATCTGCATACTTCTT+GGG + chr4.1:471219-471238 None:intergenic 45.0%
ACTTCAGCCACAGCAATTTC+CGG - chr4.1:472178-472197 MS.gene94545:CDS 45.0%
AGCCCATTGGACACAGATTT+GGG + chr4.1:471042-471061 None:intergenic 45.0%
AGGGTCGAGAGAAACGAAAA+CGG + chr4.1:470935-470954 None:intergenic 45.0%
CTCAACCACTTGAAATCTGC+TGG - chr4.1:470739-470758 MS.gene94545:CDS 45.0%
CTGTGGAGCCTGTTAAAAGA+GGG - chr4.1:470971-470990 MS.gene94545:intron 45.0%
GAGTTAGATCTGAAAGTCCC+AGG + chr4.1:471009-471028 None:intergenic 45.0%
GCTCACTTTGCAGCAATCAA+AGG - chr4.1:472028-472047 MS.gene94545:CDS 45.0%
GGATCAAGCAAAAGGGCAAT+AGG - chr4.1:470649-470668 MS.gene94545:CDS 45.0%
GTAGACCAGCAGATTTCAAG+TGG + chr4.1:470747-470766 None:intergenic 45.0%
GTGGTCAGATCTATTGAGAG+TGG + chr4.1:470821-470840 None:intergenic 45.0%
TAGCTTGGGGCTAAAGTATG+TGG + chr4.1:472416-472435 None:intergenic 45.0%
TCAGATCTATTGAGAGTGGG+AGG + chr4.1:470817-470836 None:intergenic 45.0%
TGTGATTCCGGAAATTGCTG+TGG + chr4.1:472188-472207 None:intergenic 45.0%
TTAGATCTGAAAGTCCCAGG+AGG + chr4.1:471006-471025 None:intergenic 45.0%
! AGTGAAGTTGGGTTGTTCGA+GGG + chr4.1:470554-470573 None:intergenic 45.0%
!!! ATTTTCTATAAAATAAAATA+GGG - chr4.1:471870-471889 MS.gene94545:CDS 5.0%
AAGGGAGATGAGCTTCAGAG+TGG + chr4.1:470840-470859 None:intergenic 50.0%
AGGCCCAAATCTGTGTCCAA+TGG - chr4.1:471036-471055 MS.gene94545:intron 50.0%
ATCTCAGCGTAAGGAGGATG+GGG + chr4.1:470592-470611 None:intergenic 50.0%
ATTTGGTATGGGGTTGGGCT+TGG + chr4.1:470905-470924 None:intergenic 50.0%
CACCGCATCTGCATACTTCT+TGG + chr4.1:471220-471239 None:intergenic 50.0%
CATCTCAGCGTAAGGAGGAT+GGG + chr4.1:470593-470612 None:intergenic 50.0%
CATCTGCATACTTCTTGGGG+CGG + chr4.1:471215-471234 None:intergenic 50.0%
CCGCATCTGCATACTTCTTG+GGG + chr4.1:471218-471237 None:intergenic 50.0%
CCTTTAACCCAAAGAGGTCG+TGG - chr4.1:470865-470884 MS.gene94545:CDS 50.0%
GAGCCCATTGGACACAGATT+TGG + chr4.1:471043-471062 None:intergenic 50.0%
GATCTATTGAGAGTGGGAGG+TGG + chr4.1:470814-470833 None:intergenic 50.0%
GCTGTGGAGCCTGTTAAAAG+AGG - chr4.1:470970-470989 MS.gene94545:intron 50.0%
GGCCCAAATCTGTGTCCAAT+GGG - chr4.1:471037-471056 MS.gene94545:intron 50.0%
GGGTCGAGAGAAACGAAAAC+GGG + chr4.1:470934-470953 None:intergenic 50.0%
GTTAGATCTACTAGGCCCAG+AGG - chr4.1:471189-471208 MS.gene94545:intron 50.0%
TATTGAGAGTGGGAGGTGGA+GGG + chr4.1:470810-470829 None:intergenic 50.0%
TCATCTCAGCGTAAGGAGGA+TGG + chr4.1:470594-470613 None:intergenic 50.0%
TGGGAATGCAGTTGCTGAGA+TGG + chr4.1:472230-472249 None:intergenic 50.0%
TGGGCATTTGGTATGGGGTT+GGG + chr4.1:470910-470929 None:intergenic 50.0%
TGTTGCAGCGGTTGTGATTC+CGG + chr4.1:472200-472219 None:intergenic 50.0%
TTGGATTGCTGCAACTGCAG+TGG + chr4.1:472105-472124 None:intergenic 50.0%
TTGTTCGAGGGTGAAGGGAA+CGG + chr4.1:470542-470561 None:intergenic 50.0%
TTTGGTATGGGGTTGGGCTT+GGG + chr4.1:470904-470923 None:intergenic 50.0%
! ACGTGGTGGTGAGTGAAGTT+GGG + chr4.1:470565-470584 None:intergenic 50.0%
! CAAGAAGTATGCAGATGCGG+TGG - chr4.1:471218-471237 MS.gene94545:intron 50.0%
! CCACGACCTCTTTGGGTTAA+AGG + chr4.1:470868-470887 None:intergenic 50.0%
! CCCCAAGAAGTATGCAGATG+CGG - chr4.1:471215-471234 MS.gene94545:intron 50.0%
! GAGAGAAACGAAAACGGGCT+GGG + chr4.1:470929-470948 None:intergenic 50.0%
! GAGTGAAGTTGGGTTGTTCG+AGG + chr4.1:470555-470574 None:intergenic 50.0%
! TGCGACTGCGATTGGGTTTT+AGG + chr4.1:471087-471106 None:intergenic 50.0%
! TGTTGGTTGCGACTGCGATT+GGG + chr4.1:471094-471113 None:intergenic 50.0%
! TTGGGTTGTTCGAGGGTGAA+GGG + chr4.1:470547-470566 None:intergenic 50.0%
! TTGTTGGTTGCGACTGCGAT+TGG + chr4.1:471095-471114 None:intergenic 50.0%
!! AACAAGGTGCTGTGTTCTGC+TGG + chr4.1:470709-470728 None:intergenic 50.0%
!!! GACTGCGATTGGGTTTTAGG+CGG + chr4.1:471084-471103 None:intergenic 50.0%
AATCCCTCCGGAAGGCCAAT+TGG - chr4.1:471297-471316 MS.gene94545:intron 55.0%
ACAGCAGTTGTAGGAGGAGG+AGG + chr4.1:470955-470974 None:intergenic 55.0%
ACAGGAGCAGCAGCAGCAAA+AGG + chr4.1:471126-471145 None:intergenic 55.0%
ACAGGCTCCACAGCAGTTGT+AGG + chr4.1:470964-470983 None:intergenic 55.0%
AGGACGACCACGACCTCTTT+GGG + chr4.1:470875-470894 None:intergenic 55.0%
AGGACGTCGTTTGAGCCCAT+TGG + chr4.1:471055-471074 None:intergenic 55.0%
AGGTGGAGGAGGGAGATCAA+AGG + chr4.1:470509-470528 None:intergenic 55.0%
ATCACCTTTGGGCGGCCAAT+TGG + chr4.1:471315-471334 None:intergenic 55.0%
ATTGCTGCAACTGCAGTGGC+TGG + chr4.1:472101-472120 None:intergenic 55.0%
ATTTATGGGCCTGCCCACCA+CGG + chr4.1:471280-471299 None:intergenic 55.0%
CAACTTCACTCACCACCACG+TGG - chr4.1:470564-470583 MS.gene94545:CDS 55.0%
CAGCAGTTGTAGGAGGAGGA+GGG + chr4.1:470954-470973 None:intergenic 55.0%
CCAGATGCCATTGCAGCAAC+AGG - chr4.1:472373-472392 MS.gene94545:CDS 55.0%
CCTAAGAAGCTTGCCGTGGT+GGG - chr4.1:471264-471283 MS.gene94545:intron 55.0%
CCTGTTGCTGCAATGGCATC+TGG + chr4.1:472376-472395 None:intergenic 55.0%
CGAGAGAAACGAAAACGGGC+TGG + chr4.1:470930-470949 None:intergenic 55.0%
CTATTGAGAGTGGGAGGTGG+AGG + chr4.1:470811-470830 None:intergenic 55.0%
CTGGGCATTTGGTATGGGGT+TGG + chr4.1:470911-470930 None:intergenic 55.0%
GAGCCTGTTAAAAGAGGGCG+TGG - chr4.1:470976-470995 MS.gene94545:intron 55.0%
GATGGCAGCTCTTGTTGCAG+CGG + chr4.1:472212-472231 None:intergenic 55.0%
GGCCTTCCGGAGGGATTTAT+GGG + chr4.1:471294-471313 None:intergenic 55.0%
TCCACAGCAGTTGTAGGAGG+AGG + chr4.1:470958-470977 None:intergenic 55.0%
TCCTAAGAAGCTTGCCGTGG+TGG - chr4.1:471263-471282 MS.gene94545:intron 55.0%
TCCTCCTCCTACAACTGCTG+TGG - chr4.1:470954-470973 MS.gene94545:intron 55.0%
TCGTCCTAAGAAGCTTGCCG+TGG - chr4.1:471260-471279 MS.gene94545:intron 55.0%
TGCTGCACCTGTTGCTGCAA+TGG + chr4.1:472383-472402 None:intergenic 55.0%
TGGCCTTCCGGAGGGATTTA+TGG + chr4.1:471295-471314 None:intergenic 55.0%
TGGGGATGCAGTTGCTGAGA+TGG + chr4.1:472314-472333 None:intergenic 55.0%
TGGGGATGGAGTTGCAGAGA+TGG + chr4.1:472272-472291 None:intergenic 55.0%
! AATGGGCTCAAACGACGTCC+TGG - chr4.1:471054-471073 MS.gene94545:intron 55.0%
! CACGTGGTGGTGAGTGAAGT+TGG + chr4.1:470566-470585 None:intergenic 55.0%
! CGTCCACGCCCTCTTTTAAC+AGG + chr4.1:470982-471001 None:intergenic 55.0%
! GTTGGGTTGTTCGAGGGTGA+AGG + chr4.1:470548-470567 None:intergenic 55.0%
!! AACGGGCTGGGCATTTGGTA+TGG + chr4.1:470917-470936 None:intergenic 55.0%
!! ACGAAAACGGGCTGGGCATT+TGG + chr4.1:470922-470941 None:intergenic 55.0%
!! ACGGGCTGGGCATTTGGTAT+GGG + chr4.1:470916-470935 None:intergenic 55.0%
AAGGCCAATTGGCCGCCCAA+AGG - chr4.1:471308-471327 MS.gene94545:intron 60.0%
AGGGTGAAGGGAACGGTGGT+GGG + chr4.1:470535-470554 None:intergenic 60.0%
AGTTGCTGAGATGGCAGCGC+CGG + chr4.1:472305-472324 None:intergenic 60.0%
ATACTTCTTGGGGCGGCCTC+TGG + chr4.1:471208-471227 None:intergenic 60.0%
ATGCGGTGGTTCCAGCGACA+AGG - chr4.1:471232-471251 MS.gene94545:intron 60.0%
ATGGGGTTGGGCTTGGGCTT+AGG + chr4.1:470898-470917 None:intergenic 60.0%
CCCACCACGGCAAGCTTCTT+AGG + chr4.1:471267-471286 None:intergenic 60.0%
CTGTGCCTGTGGCACTTGCT+GGG + chr4.1:472333-472352 None:intergenic 60.0%
GAGGACGACCACGACCTCTT+TGG + chr4.1:470876-470895 None:intergenic 60.0%
GATGGCAGCGACTGTGACAC+TGG + chr4.1:472254-472273 None:intergenic 60.0%
GGCAGGCCCATAAATCCCTC+CGG - chr4.1:471285-471304 MS.gene94545:intron 60.0%
GGCCCATAAATCCCTCCGGA+AGG - chr4.1:471289-471308 MS.gene94545:intron 60.0%
GGCTCCACAGCAGTTGTAGG+AGG + chr4.1:470961-470980 None:intergenic 60.0%
TACTTCTTGGGGCGGCCTCT+GGG + chr4.1:471207-471226 None:intergenic 60.0%
TCTGTGCCTGTGGCACTTGC+TGG + chr4.1:472334-472353 None:intergenic 60.0%
TTCGAGGGTGAAGGGAACGG+TGG + chr4.1:470539-470558 None:intergenic 60.0%
! CAGCGACTGTGACACTGGCT+GGG + chr4.1:472249-472268 None:intergenic 60.0%
! CGGGCTGGGCATTTGGTATG+GGG + chr4.1:470915-470934 None:intergenic 60.0%
! TGGTGGGTAGAGGTGGAGGA+GGG + chr4.1:470519-470538 None:intergenic 60.0%
! TGTGCCTGTGGCACTTGCTG+GGG + chr4.1:472332-472351 None:intergenic 60.0%
!! AAGGGAACGGTGGTGGGTAG+AGG + chr4.1:470529-470548 None:intergenic 60.0%
!!! TGGGTTTTAGGCGGACGACC+AGG + chr4.1:471075-471094 None:intergenic 60.0%
AGTGGGAGGTGGAGGGAGTG+AGG + chr4.1:470803-470822 None:intergenic 65.0%
CCATCCCCAACCAGTGCCAC+CGG - chr4.1:472283-472302 MS.gene94545:CDS 65.0%
CGGTGGTTCCAGCGACAAGG+CGG - chr4.1:471235-471254 MS.gene94545:intron 65.0%
GAGGGTGAAGGGAACGGTGG+TGG + chr4.1:470536-470555 None:intergenic 65.0%
GCAGCGACTGTGACACTGGC+TGG + chr4.1:472250-472269 None:intergenic 65.0%
GCATCCCCAGCAAGTGCCAC+AGG - chr4.1:472325-472344 MS.gene94545:CDS 65.0%
GGAGGTGGAGGGAGTGAGGA+AGG + chr4.1:470799-470818 None:intergenic 65.0%
GGGTTGGGCTTGGGCTTAGG+AGG + chr4.1:470895-470914 None:intergenic 65.0%
GGTGGTTCCAGCGACAAGGC+GGG - chr4.1:471236-471255 MS.gene94545:intron 65.0%
GTGGTTCCAGCGACAAGGCG+GGG - chr4.1:471237-471256 MS.gene94545:intron 65.0%
! AAGGAGGATGGGGTGCCACG+TGG + chr4.1:470582-470601 None:intergenic 65.0%
!! ACGGTGGTGGGTAGAGGTGG+AGG + chr4.1:470523-470542 None:intergenic 65.0%
!! AGAAGCTTGCCGTGGTGGGC+AGG - chr4.1:471268-471287 MS.gene94545:intron 65.0%
!! CGGCCAATTGGCCTTCCGGA+GGG + chr4.1:471303-471322 None:intergenic 65.0%
!! GGAACGGTGGTGGGTAGAGG+TGG + chr4.1:470526-470545 None:intergenic 65.0%
!! GTGGTGGGTAGAGGTGGAGG+AGG + chr4.1:470520-470539 None:intergenic 65.0%
!! TGGGCGGCCAATTGGCCTTC+CGG + chr4.1:471307-471326 None:intergenic 65.0%
! AGAGGGCGTGGACGTCCTCC+TGG - chr4.1:470988-471007 MS.gene94545:CDS 70.0%
! GAGGATGGGGTGCCACGTGG+TGG + chr4.1:470579-470598 None:intergenic 70.0%
! GAGGGCGTGGACGTCCTCCT+GGG - chr4.1:470989-471008 MS.gene94545:CDS 70.0%
! TGCTGAGATGGCAGCGCCGG+TGG + chr4.1:472302-472321 None:intergenic 70.0%
!! AGCGCCGGTGGCACTGGTTG+GGG + chr4.1:472290-472309 None:intergenic 70.0%
!! CAGCGCCGGTGGCACTGGTT+GGG + chr4.1:472291-472310 None:intergenic 70.0%
!! CCGGTGGCACTGGTTGGGGA+TGG + chr4.1:472286-472305 None:intergenic 70.0%
!! GCGGCCAATTGGCCTTCCGG+AGG + chr4.1:471304-471323 None:intergenic 70.0%
GGACGACCCCGCCTTGTCGC+TGG + chr4.1:471246-471265 None:intergenic 75.0%
!! GATGGCAGCGCCGGTGGCAC+TGG + chr4.1:472296-472315 None:intergenic 75.0%
!! GCAGCGCCGGTGGCACTGGT+TGG + chr4.1:472292-472311 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 470493 472436 470493 ID=MS.gene94545
chr4.1 mRNA 470493 472436 470493 ID=MS.gene94545.t1;Parent=MS.gene94545
chr4.1 exon 471600 472436 471600 ID=MS.gene94545.t1.exon1;Parent=MS.gene94545.t1
chr4.1 CDS 471600 472436 471600 ID=cds.MS.gene94545.t1;Parent=MS.gene94545.t1
chr4.1 exon 470982 471038 470982 ID=MS.gene94545.t1.exon2;Parent=MS.gene94545.t1
chr4.1 CDS 470982 471038 470982 ID=cds.MS.gene94545.t1;Parent=MS.gene94545.t1
chr4.1 exon 470493 470888 470493 ID=MS.gene94545.t1.exon3;Parent=MS.gene94545.t1
chr4.1 CDS 470493 470888 470493 ID=cds.MS.gene94545.t1;Parent=MS.gene94545.t1
Gene Sequence

>MS.gene94545

ATGGACTCAACTTCCTTTGATCTCCCTCCTCCACCTCTACCCACCACCGTTCCCTTCACCCTCGAACAACCCAACTTCACTCACCACCACGTGGCACCCCATCCTCCTTACGCTGAGATGATATACAAAGCAATTGAAGCACTGAAAGAGAAAGATGGATCAAGCAAAAGGGCAATAGGAAAGTACATAGAACATGTCTACAAACAAGTTCTACCAGCAGAACACAGCACCTTGTTAACACAACATCTCAACCACTTGAAATCTGCTGGTCTACTAATCATGTTCAAAAAATCATACAAACTACCTTCCTCACTCCCTCCACCTCCCACTCTCAATAGATCTGACCACTCTGAAGCTCATCTCCCTTTCACTCCTTTAACCCAAAGAGGTCGTGGTCGTCCTCCTAAGCCCAAGCCCAACCCCATACCAAATGCCCAGCCCGTTTTCGTTTCTCTCGACCCTCCTCCTCCTACAACTGCTGTGGAGCCTGTTAAAAGAGGGCGTGGACGTCCTCCTGGGACTTTCAGATCTAACTCGATAAAGAGGCCCAAATCTGTGTCCAATGGGCTCAAACGACGTCCTGGTCGTCCGCCTAAAACCCAATCGCAGTCGCAACCAACAACTGTCATACCTTTTGCTGCTGCTGCTCCTGTTCCCTTTCCTTCTGTTGTTCCAGAAGATGAAGCCAATGTATCTGTTAGATCTACTAGGCCCAGAGGCCGCCCCAAGAAGTATGCAGATGCGGTGGTTCCAGCGACAAGGCGGGGTCGTCCTAAGAAGCTTGCCGTGGTGGGCAGGCCCATAAATCCCTCCGGAAGGCCAATTGGCCGCCCAAAGGTGATCCTAATGTTGAGTTTTTATTGTTTCCTTCATCTATTTATGTGTGTTTTACTACTACTACTACTACTTAATTTGACTTGCCTTATTTTGGTCTAGTATGAGATTATGGGTATTTAATTACTGTAAGTCCCTAAATGATTAACTATTTAATGTTAGTGTATTTAAGCTTATCCACATTGACTTTTGGAAACAAAACACATTTATCTTGAAAACGAAGGCATGTCATATATGGCATGTGTGTTCGTGTAATAAACAATAAAGCAGATGGATAAAATTGTTTTAAAAAAAAAAAATTCTGTTTCATTTTGTACTACTAGTAATTAAGCAAGTAGAGAGGGTAAAGAAATATTTTGCATGTAGTATTATTATTTTGCATCAATGAATAAGCAAATAATAAATTCAATTTGTTGTCTTTGGAACATATTTGGACAAATGTTAACATTCTCGAGCTATGTGGATTGTGCGTTTCTGTTAAAAGAGAGATATTTTAACATACTTTGCTTTCCTGTATAAATTAAAGATTTTTTCTTATCTTTCATTTTCTATAAAATAAAATAGGGAGCAAAAGGTAAAAAGTTTGTCAATGAAGATCTAAGAAGGAAGCTTGAACACTTTGTAAGTTGAATTTATCTTTCATTTGGTTGCTCGTTATCTTTCAAATTAAACAAATGATATATCTCATGCCTCTATTGTTTGCTCACTTTGCAGCAATCAAAGGTTAAGGAATCTCTTGACGTGCTTAAGCCTCTCTTTGATCATCAAAGTCCAGCCACTGCAGTTGCAGCAATCCAAGAGTTAGAAATGCTGGCAAGTTTGGACCTTAAAGCACCATTAAGAGATGAAACACTTCAGCCACAGCAATTTCCGGAATCACAACCGCTGCAACAAGAGCTGCCATCTCAGCAACTGCATTCCCAGCCAGTGTCACAGTCGCTGCCATCTCTGCAACTCCATCCCCAACCAGTGCCACCGGCGCTGCCATCTCAGCAACTGCATCCCCAGCAAGTGCCACAGGCACAGAATTATGAACAGCAATATCTCCAGATGCCATTGCAGCAACAGGTGCAGCAGCTGTTTCAACCACATACTTTAGCCCCAAGCTAG
Protein sequence

>MS.gene94545.t1

MDSTSFDLPPPPLPTTVPFTLEQPNFTHHHVAPHPPYAEMIYKAIEALKEKDGSSKRAIGKYIEHVYKQVLPAEHSTLLTQHLNHLKSAGLLIMFKKSYKLPSSLPPPPTLNRSDHSEAHLPFTPLTQRGRGRPPKPKPNPIPNAQPVFVSLDPPPPTTAVEPVKRGRGRPPGTFRSNSIKRPKSVSNGLKRRPGRPPKTQSQSQPTTVIPFAAAAPVPFPSVVPEDEANVSVRSTRPRGRPKKYADAVVPATRRGRPKKLAVVGRPINPSGRPIGRPKGAKGKKFVNEDLRRKLEHFQSKVKESLDVLKPLFDHQSPATAVAAIQELEMLASLDLKAPLRDETLQPQQFPESQPLQQELPSQQLHSQPVSQSLPSLQLHPQPVPPALPSQQLHPQQVPQAQNYEQQYLQMPLQQQVQQLFQPHTLAPS