Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94922.t1 | RHN67368.1 | 93.6 | 204 | 13 | 0 | 3 | 206 | 546 | 749 | 1.30E-99 | 372.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94922.t1 | A0A072UW38 | 93.6 | 204 | 13 | 0 | 3 | 206 | 189 | 392 | 9.3e-100 | 372.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058168 | MS.gene94922 | 0.821523 | 3.86E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94922.t1 | MTR_3g058570 | 93.627 | 204 | 13 | 0 | 3 | 206 | 189 | 392 | 1.99e-131 | 375 |
| MS.gene94922.t1 | MTR_5g085340 | 67.647 | 204 | 65 | 1 | 3 | 206 | 550 | 752 | 2.81e-87 | 272 |
| MS.gene94922.t1 | MTR_5g085350 | 67.157 | 204 | 66 | 1 | 3 | 206 | 529 | 731 | 2.61e-86 | 269 |
| MS.gene94922.t1 | MTR_5g085350 | 67.157 | 204 | 66 | 1 | 3 | 206 | 542 | 744 | 3.29e-86 | 269 |
| MS.gene94922.t1 | MTR_5g085360 | 57.286 | 199 | 83 | 1 | 3 | 201 | 542 | 738 | 1.98e-74 | 238 |
| MS.gene94922.t1 | MTR_3g051060 | 53.695 | 203 | 91 | 2 | 3 | 203 | 538 | 739 | 4.36e-69 | 224 |
| MS.gene94922.t1 | MTR_2g020510 | 52.332 | 193 | 92 | 0 | 6 | 198 | 493 | 685 | 2.87e-60 | 200 |
| MS.gene94922.t1 | MTR_2g020470 | 49.223 | 193 | 98 | 0 | 6 | 198 | 475 | 667 | 3.89e-58 | 194 |
| MS.gene94922.t1 | MTR_3g050500 | 51.777 | 197 | 92 | 2 | 3 | 197 | 533 | 728 | 5.00e-57 | 192 |
| MS.gene94922.t1 | MTR_2g020450 | 50.259 | 193 | 95 | 1 | 6 | 198 | 496 | 687 | 7.78e-51 | 174 |
| MS.gene94922.t1 | MTR_3g055310 | 37.931 | 174 | 107 | 1 | 10 | 183 | 164 | 336 | 7.68e-37 | 131 |
| MS.gene94922.t1 | MTR_3g055310 | 37.931 | 174 | 107 | 1 | 10 | 183 | 227 | 399 | 2.80e-36 | 131 |
| MS.gene94922.t1 | MTR_3g055310 | 37.931 | 174 | 107 | 1 | 10 | 183 | 279 | 451 | 6.48e-36 | 131 |
| MS.gene94922.t1 | MTR_3g055310 | 37.931 | 174 | 107 | 1 | 10 | 183 | 307 | 479 | 7.14e-36 | 131 |
| MS.gene94922.t1 | MTR_3g055310 | 37.931 | 174 | 107 | 1 | 10 | 183 | 305 | 477 | 1.06e-35 | 131 |
| MS.gene94922.t1 | MTR_5g091220 | 39.444 | 180 | 108 | 1 | 10 | 189 | 420 | 598 | 1.14e-35 | 132 |
| MS.gene94922.t1 | MTR_2g438740 | 37.766 | 188 | 116 | 1 | 2 | 189 | 377 | 563 | 1.36e-35 | 131 |
| MS.gene94922.t1 | MTR_3g055310 | 37.931 | 174 | 107 | 1 | 10 | 183 | 332 | 504 | 1.78e-35 | 130 |
| MS.gene94922.t1 | MTR_5g028130 | 37.500 | 176 | 109 | 1 | 18 | 193 | 162 | 336 | 7.98e-35 | 126 |
| MS.gene94922.t1 | MTR_2g017930 | 38.605 | 215 | 114 | 4 | 9 | 206 | 461 | 674 | 6.74e-34 | 127 |
| MS.gene94922.t1 | MTR_5g028190 | 38.068 | 176 | 108 | 1 | 18 | 193 | 358 | 532 | 7.22e-34 | 126 |
| MS.gene94922.t1 | MTR_6g017215 | 35.135 | 185 | 119 | 1 | 3 | 187 | 6 | 189 | 1.38e-32 | 117 |
| MS.gene94922.t1 | MTR_2g438400 | 37.791 | 172 | 106 | 1 | 18 | 189 | 371 | 541 | 4.43e-32 | 122 |
| MS.gene94922.t1 | MTR_2g438440 | 38.372 | 172 | 105 | 1 | 18 | 189 | 512 | 682 | 7.79e-30 | 116 |
| MS.gene94922.t1 | MTR_2g438700 | 34.555 | 191 | 123 | 2 | 2 | 192 | 501 | 689 | 9.58e-30 | 115 |
| MS.gene94922.t1 | MTR_2g438720 | 34.574 | 188 | 121 | 2 | 2 | 189 | 503 | 688 | 1.78e-29 | 115 |
| MS.gene94922.t1 | MTR_3g455790 | 37.278 | 169 | 104 | 2 | 10 | 178 | 415 | 581 | 1.53e-28 | 112 |
| MS.gene94922.t1 | MTR_2g438640 | 33.862 | 189 | 123 | 2 | 2 | 190 | 502 | 688 | 5.42e-27 | 107 |
| MS.gene94922.t1 | MTR_2g438640 | 34.595 | 185 | 119 | 2 | 6 | 190 | 403 | 585 | 5.56e-27 | 107 |
| MS.gene94922.t1 | MTR_2g438640 | 33.684 | 190 | 124 | 2 | 2 | 191 | 476 | 663 | 5.71e-27 | 107 |
| MS.gene94922.t1 | MTR_5g028450 | 35.484 | 186 | 117 | 1 | 8 | 190 | 107 | 292 | 4.58e-26 | 102 |
| MS.gene94922.t1 | MTR_3g452020 | 33.149 | 181 | 117 | 3 | 10 | 190 | 240 | 416 | 1.61e-24 | 99.8 |
| MS.gene94922.t1 | MTR_3g452020 | 33.149 | 181 | 117 | 3 | 10 | 190 | 416 | 592 | 2.72e-24 | 100 |
| MS.gene94922.t1 | MTR_2g438760 | 30.168 | 179 | 120 | 2 | 16 | 191 | 384 | 560 | 3.40e-23 | 97.1 |
| MS.gene94922.t1 | MTR_5g028930 | 40.769 | 130 | 76 | 1 | 18 | 147 | 438 | 566 | 1.79e-22 | 94.7 |
| MS.gene94922.t1 | MTR_5g028690 | 36.842 | 133 | 84 | 0 | 18 | 150 | 56 | 188 | 5.01e-22 | 90.5 |
| MS.gene94922.t1 | MTR_5g028900 | 35.915 | 142 | 79 | 3 | 18 | 159 | 17 | 146 | 3.96e-20 | 83.2 |
| MS.gene94922.t1 | MTR_5g028110 | 31.973 | 147 | 99 | 1 | 47 | 193 | 28 | 173 | 1.45e-19 | 82.4 |
| MS.gene94922.t1 | MTR_5g028840 | 33.784 | 148 | 98 | 0 | 3 | 150 | 2 | 149 | 2.95e-19 | 82.0 |
| MS.gene94922.t1 | MTR_2g438580 | 40.506 | 79 | 46 | 1 | 18 | 96 | 3 | 80 | 1.71e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94922.t1 | AT5G04730 | 37.755 | 196 | 121 | 1 | 6 | 201 | 403 | 597 | 3.06e-34 | 128 |
| MS.gene94922.t1 | AT3G54065 | 34.715 | 193 | 120 | 2 | 6 | 192 | 25 | 217 | 7.72e-34 | 120 |
| MS.gene94922.t1 | AT5G35810 | 34.896 | 192 | 118 | 3 | 6 | 191 | 140 | 330 | 1.60e-33 | 122 |
| MS.gene94922.t1 | AT5G04700 | 39.344 | 183 | 110 | 1 | 8 | 189 | 470 | 652 | 1.71e-33 | 126 |
| MS.gene94922.t1 | AT3G18670 | 36.788 | 193 | 119 | 2 | 10 | 202 | 402 | 591 | 1.70e-32 | 123 |
| MS.gene94922.t1 | AT3G18670 | 36.788 | 193 | 119 | 2 | 10 | 202 | 457 | 646 | 2.45e-32 | 123 |
| MS.gene94922.t1 | AT5G04690 | 38.830 | 188 | 102 | 3 | 8 | 189 | 428 | 608 | 2.79e-32 | 122 |
| MS.gene94922.t1 | AT5G04690 | 38.830 | 188 | 102 | 3 | 8 | 189 | 462 | 642 | 3.89e-32 | 122 |
| MS.gene94922.t1 | AT5G04690 | 38.830 | 188 | 102 | 3 | 8 | 189 | 448 | 628 | 4.06e-32 | 122 |
| MS.gene94922.t1 | AT5G04680 | 36.979 | 192 | 110 | 3 | 8 | 189 | 334 | 524 | 4.15e-27 | 107 |
| MS.gene94922.t1 | AT5G04680 | 36.979 | 192 | 110 | 3 | 8 | 189 | 457 | 647 | 6.48e-27 | 107 |
| MS.gene94922.t1 | AT5G04680 | 36.979 | 192 | 110 | 3 | 8 | 189 | 486 | 676 | 6.57e-27 | 107 |
| MS.gene94922.t1 | AT5G04680 | 36.979 | 192 | 110 | 3 | 8 | 189 | 443 | 633 | 6.98e-27 | 107 |
Find 38 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCTTTGTCTTCAGTAAT+TGG | 0.161186 | 3.1:+43789664 | MS.gene94922:CDS |
| ACACAATGTGCTTGTATTTA+TGG | 0.189985 | 3.1:-43789864 | None:intergenic |
| TTTACCAAGGAAACTTCTTT+TGG | 0.277654 | 3.1:+43789768 | MS.gene94922:CDS |
| TAAATCAATTCTGAAATCTT+TGG | 0.278020 | 3.1:-43789749 | None:intergenic |
| CTTTACATTGATCTTCTTAG+AGG | 0.299445 | 3.1:+43789946 | MS.gene94922:CDS |
| CATGAAGAACTGGTACAAAA+AGG | 0.353958 | 3.1:+43789490 | MS.gene94922:CDS |
| GTAGTAGCAGCACTCCTTGC+CGG | 0.383621 | 3.1:+43789550 | MS.gene94922:CDS |
| TTCCTTTCTAGAAGTGAGTA+TGG | 0.391143 | 3.1:-43789725 | None:intergenic |
| GGCGGCAACAGAAGTGAAAC+AGG | 0.413726 | 3.1:+43789604 | MS.gene94922:CDS |
| GTGCCAAAGTCACGTGATAA+GGG | 0.414563 | 3.1:+43789982 | MS.gene94922:CDS |
| AGTGCCAAAGTCACGTGATA+AGG | 0.414651 | 3.1:+43789981 | MS.gene94922:CDS |
| AATCCCAAAAGAAGTTTCCT+TGG | 0.430549 | 3.1:-43789772 | None:intergenic |
| CAAGAAATCACATGAAGAAC+TGG | 0.431741 | 3.1:+43789480 | MS.gene94922:CDS |
| GTTTCACTTCTGTTGCCGCC+TGG | 0.452202 | 3.1:-43789601 | None:intergenic |
| GCAGGCCTTCCTTCCAATGC+TGG | 0.454814 | 3.1:-43789631 | None:intergenic |
| TGAAACAGGCGAACCAGCAT+TGG | 0.463366 | 3.1:+43789618 | MS.gene94922:CDS |
| TATGGTCAATCAGAAAGAAA+TGG | 0.463630 | 3.1:-43789846 | None:intergenic |
| ATCACGTGACTTTGGCACTT+TGG | 0.464104 | 3.1:-43789977 | None:intergenic |
| GTTCTAACTGTGAAATGCTC+TGG | 0.475307 | 3.1:-43789424 | None:intergenic |
| AACAAAGAAGACAAAACAGC+TGG | 0.494490 | 3.1:+43789448 | MS.gene94922:CDS |
| CTAAGAAGATCAATGTAAAG+TGG | 0.497918 | 3.1:-43789943 | None:intergenic |
| CACATGGATCAGTATACTAA+AGG | 0.504814 | 3.1:+43789394 | None:intergenic |
| GTCCATACTCACTTCTAGAA+AGG | 0.511769 | 3.1:+43789723 | MS.gene94922:CDS |
| TTCTAGAAGTGAGTATGGAC+AGG | 0.525171 | 3.1:-43789720 | None:intergenic |
| GCGAACCAGCATTGGAAGGA+AGG | 0.537330 | 3.1:+43789626 | MS.gene94922:CDS |
| AGCGACGAACATTGCAACAA+TGG | 0.549560 | 3.1:-43789812 | None:intergenic |
| GCTACTGCATACAAAGTCAC+AGG | 0.569943 | 3.1:-43789913 | None:intergenic |
| ATTTCAGAATTGATTTACCA+AGG | 0.574578 | 3.1:+43789755 | MS.gene94922:CDS |
| GCTACATCAAGCACTGTACC+AGG | 0.575420 | 3.1:+43789583 | MS.gene94922:CDS |
| AAAGCAAATGCATCAAATGC+AGG | 0.578955 | 3.1:-43789649 | None:intergenic |
| TACAGTGCTTGATGTAGCGA+AGG | 0.603317 | 3.1:-43789578 | None:intergenic |
| TGCCAAAGTCACGTGATAAG+GGG | 0.611312 | 3.1:+43789983 | MS.gene94922:CDS |
| ACAGGCGAACCAGCATTGGA+AGG | 0.613779 | 3.1:+43789622 | MS.gene94922:CDS |
| GCCAAAGTCACGTGATAAGG+GGG | 0.625779 | 3.1:+43789984 | MS.gene94922:CDS |
| TGATGTAGCGAAGGAAACAC+CGG | 0.642955 | 3.1:-43789569 | None:intergenic |
| TAGCGAAGGAAACACCGGCA+AGG | 0.651641 | 3.1:-43789564 | None:intergenic |
| CTACTGCATACAAAGTCACA+GGG | 0.663393 | 3.1:-43789912 | None:intergenic |
| ACATCAAGCACTGTACCAGG+CGG | 0.758516 | 3.1:+43789586 | MS.gene94922:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATCAATTCTGAAATCTT+TGG | - | chr3.1:43789752-43789771 | None:intergenic | 20.0% |
| !! | ATTTCAGAATTGATTTACCA+AGG | + | chr3.1:43789755-43789774 | MS.gene94922:CDS | 25.0% |
| ACACAATGTGCTTGTATTTA+TGG | - | chr3.1:43789867-43789886 | None:intergenic | 30.0% | |
| CTAAGAAGATCAATGTAAAG+TGG | - | chr3.1:43789946-43789965 | None:intergenic | 30.0% | |
| CTTTACATTGATCTTCTTAG+AGG | + | chr3.1:43789946-43789965 | MS.gene94922:CDS | 30.0% | |
| TATGGTCAATCAGAAAGAAA+TGG | - | chr3.1:43789849-43789868 | None:intergenic | 30.0% | |
| TTTGCTTTGTCTTCAGTAAT+TGG | + | chr3.1:43789664-43789683 | MS.gene94922:CDS | 30.0% | |
| ! | TTACCAAGGAAACTTCTTTT+GGG | + | chr3.1:43789769-43789788 | MS.gene94922:CDS | 30.0% |
| ! | TTTACCAAGGAAACTTCTTT+TGG | + | chr3.1:43789768-43789787 | MS.gene94922:CDS | 30.0% |
| AAAGCAAATGCATCAAATGC+AGG | - | chr3.1:43789652-43789671 | None:intergenic | 35.0% | |
| AACAAAGAAGACAAAACAGC+TGG | + | chr3.1:43789448-43789467 | MS.gene94922:CDS | 35.0% | |
| AATCCCAAAAGAAGTTTCCT+TGG | - | chr3.1:43789775-43789794 | None:intergenic | 35.0% | |
| CAAGAAATCACATGAAGAAC+TGG | + | chr3.1:43789480-43789499 | MS.gene94922:CDS | 35.0% | |
| CATGAAGAACTGGTACAAAA+AGG | + | chr3.1:43789490-43789509 | MS.gene94922:CDS | 35.0% | |
| ! | TTCCTTTCTAGAAGTGAGTA+TGG | - | chr3.1:43789728-43789747 | None:intergenic | 35.0% |
| CTACTGCATACAAAGTCACA+GGG | - | chr3.1:43789915-43789934 | None:intergenic | 40.0% | |
| GTTCTAACTGTGAAATGCTC+TGG | - | chr3.1:43789427-43789446 | None:intergenic | 40.0% | |
| TGGTACAAAAAGGCAGTGAA+TGG | + | chr3.1:43789500-43789519 | MS.gene94922:CDS | 40.0% | |
| ! | GTCCATACTCACTTCTAGAA+AGG | + | chr3.1:43789723-43789742 | MS.gene94922:CDS | 40.0% |
| ! | TTCTAGAAGTGAGTATGGAC+AGG | - | chr3.1:43789723-43789742 | None:intergenic | 40.0% |
| AGCGACGAACATTGCAACAA+TGG | - | chr3.1:43789815-43789834 | None:intergenic | 45.0% | |
| AGTGCCAAAGTCACGTGATA+AGG | + | chr3.1:43789981-43790000 | MS.gene94922:CDS | 45.0% | |
| ATCACGTGACTTTGGCACTT+TGG | - | chr3.1:43789980-43789999 | None:intergenic | 45.0% | |
| GCTACTGCATACAAAGTCAC+AGG | - | chr3.1:43789916-43789935 | None:intergenic | 45.0% | |
| GTGCCAAAGTCACGTGATAA+GGG | + | chr3.1:43789982-43790001 | MS.gene94922:CDS | 45.0% | |
| TACAGTGCTTGATGTAGCGA+AGG | - | chr3.1:43789581-43789600 | None:intergenic | 45.0% | |
| TGATGTAGCGAAGGAAACAC+CGG | - | chr3.1:43789572-43789591 | None:intergenic | 45.0% | |
| TGCCAAAGTCACGTGATAAG+GGG | + | chr3.1:43789983-43790002 | MS.gene94922:CDS | 45.0% | |
| !!! | ATGTTCGTCGCTTTTTGCTC+TGG | + | chr3.1:43789823-43789842 | MS.gene94922:CDS | 45.0% |
| AAAGTCACGTGATAAGGGGG+AGG | + | chr3.1:43789987-43790006 | MS.gene94922:CDS | 50.0% | |
| GCCAAAGTCACGTGATAAGG+GGG | + | chr3.1:43789984-43790003 | MS.gene94922:CDS | 50.0% | |
| TCCCCCTTATCACGTGACTT+TGG | - | chr3.1:43789988-43790007 | None:intergenic | 50.0% | |
| TGAAACAGGCGAACCAGCAT+TGG | + | chr3.1:43789618-43789637 | MS.gene94922:CDS | 50.0% | |
| ! | ACATCAAGCACTGTACCAGG+CGG | + | chr3.1:43789586-43789605 | MS.gene94922:CDS | 50.0% |
| ! | GCTACATCAAGCACTGTACC+AGG | + | chr3.1:43789583-43789602 | MS.gene94922:CDS | 50.0% |
| GGCGGCAACAGAAGTGAAAC+AGG | + | chr3.1:43789604-43789623 | MS.gene94922:CDS | 55.0% | |
| GTAGTAGCAGCACTCCTTGC+CGG | + | chr3.1:43789550-43789569 | MS.gene94922:CDS | 55.0% | |
| GTTTCACTTCTGTTGCCGCC+TGG | - | chr3.1:43789604-43789623 | None:intergenic | 55.0% | |
| TAGCGAAGGAAACACCGGCA+AGG | - | chr3.1:43789567-43789586 | None:intergenic | 55.0% | |
| ! | ACAGGCGAACCAGCATTGGA+AGG | + | chr3.1:43789622-43789641 | MS.gene94922:CDS | 55.0% |
| ! | GCGAACCAGCATTGGAAGGA+AGG | + | chr3.1:43789626-43789645 | MS.gene94922:CDS | 55.0% |
| GCAGGCCTTCCTTCCAATGC+TGG | - | chr3.1:43789634-43789653 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 43789397 | 43790017 | 43789397 | ID=MS.gene94922 |
| chr3.1 | mRNA | 43789397 | 43790017 | 43789397 | ID=MS.gene94922.t1;Parent=MS.gene94922 |
| chr3.1 | exon | 43789397 | 43790017 | 43789397 | ID=MS.gene94922.t1.exon1;Parent=MS.gene94922.t1 |
| chr3.1 | CDS | 43789397 | 43790017 | 43789397 | ID=cds.MS.gene94922.t1;Parent=MS.gene94922.t1 |
| Gene Sequence |
| Protein sequence |
