AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95060.t1 MTR_4g134490 96.944 360 11 0 1 360 14 373 0.0 733
MS.gene95060.t1 MTR_4g134490 95.355 366 11 1 1 360 14 379 0.0 726
MS.gene95060.t1 MTR_8g096880 78.841 345 73 0 9 353 3 347 0.0 591
MS.gene95060.t1 MTR_8g096880 78.193 321 70 0 9 329 3 323 0.0 539
MS.gene95060.t1 MTR_5g009170 67.151 344 111 2 9 352 44 385 1.80e-173 488
MS.gene95060.t1 MTR_8g096880 78.082 292 64 0 9 300 3 294 5.50e-173 483
MS.gene95060.t1 MTR_8g099535 67.449 341 109 2 12 352 4 342 4.25e-171 480
MS.gene95060.t1 MTR_5g009160 65.982 341 114 2 12 352 4 342 9.11e-169 474
MS.gene95060.t1 MTR_5g009160 68.030 269 85 1 84 352 12 279 5.45e-137 391
MS.gene95060.t1 MTR_5g009160 66.820 217 71 1 84 300 12 227 4.43e-104 306
MS.gene95060.t1 MTR_2g032100 41.311 351 180 10 14 352 73 409 1.66e-82 257
MS.gene95060.t1 MTR_8g089180 40.510 353 184 10 14 354 73 411 1.65e-78 247
MS.gene95060.t1 MTR_8g089180 40.510 353 184 10 14 354 73 411 1.65e-78 247
MS.gene95060.t1 MTR_2g032100 42.857 196 107 5 14 208 73 264 2.97e-46 160
MS.gene95060.t1 MTR_8g099285 74.545 55 13 1 244 298 3 56 6.35e-21 85.5
MS.gene95060.t1 MTR_1g009600 25.212 353 223 13 10 351 3 325 1.45e-19 90.5
MS.gene95060.t1 MTR_2g073240 24.786 351 223 14 12 351 19 339 4.62e-19 89.0
MS.gene95060.t1 MTR_0034s0060 23.714 350 228 13 12 351 137 457 3.58e-17 83.6
MS.gene95060.t1 MTR_4g102270 25.424 354 234 11 3 344 84 419 2.21e-16 80.5
MS.gene95060.t1 MTR_4g120030 27.300 337 195 13 7 336 106 399 2.53e-16 80.1
MS.gene95060.t1 MTR_4g129820 24.789 355 235 10 3 344 86 421 4.76e-15 76.3
MS.gene95060.t1 MTR_5g011940 24.781 343 230 10 13 344 91 416 5.64e-15 76.3
MS.gene95060.t1 MTR_1g088480 26.667 330 192 13 14 336 120 406 1.25e-13 72.0
MS.gene95060.t1 MTR_1g067530 26.705 352 207 17 11 336 111 437 5.21e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95060.t1 AT1G12780 78.510 349 75 0 8 356 3 351 0.0 592
MS.gene95060.t1 AT1G63180 76.301 346 82 0 8 353 3 348 0.0 580
MS.gene95060.t1 AT4G10960 66.667 342 112 2 12 353 4 343 1.22e-174 489
MS.gene95060.t1 AT4G23920 66.864 338 110 2 12 349 3 338 6.52e-171 480
MS.gene95060.t1 AT1G64440 66.667 339 111 2 14 352 5 341 1.72e-170 479
MS.gene95060.t1 AT1G63180 75.258 291 72 0 8 298 3 293 5.48e-169 473
MS.gene95060.t1 AT5G44480 38.857 350 190 8 14 352 73 409 2.84e-78 246
MS.gene95060.t1 AT5G44480 38.857 350 190 8 14 352 73 409 2.84e-78 246
MS.gene95060.t1 AT5G44480 38.857 350 190 8 14 352 73 409 2.84e-78 246
MS.gene95060.t1 AT5G44480 38.857 350 190 8 14 352 97 433 8.13e-78 246
MS.gene95060.t1 AT4G20460 38.000 350 193 7 14 352 72 408 1.54e-76 242
MS.gene95060.t1 AT1G30620 38.352 352 193 8 14 354 72 410 1.98e-74 236
MS.gene95060.t1 AT1G30620 38.352 352 193 8 14 354 73 411 2.22e-74 236
MS.gene95060.t1 AT1G30620 38.352 352 193 8 14 354 73 411 2.22e-74 236
MS.gene95060.t1 AT1G30620 38.352 352 193 8 14 354 73 411 2.22e-74 236
MS.gene95060.t1 AT1G30620 38.352 352 193 8 14 354 73 411 2.22e-74 236
MS.gene95060.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.12e-42 147
MS.gene95060.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.12e-42 147
MS.gene95060.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.12e-42 147
MS.gene95060.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.12e-42 147
MS.gene95060.t1 AT1G30620 38.974 195 116 3 14 208 73 264 1.92e-41 147
MS.gene95060.t1 AT1G53500 24.571 350 225 13 12 351 9 329 6.58e-19 88.6
MS.gene95060.t1 AT3G14790 24.370 357 231 12 5 351 3 330 6.76e-19 88.6
MS.gene95060.t1 AT3G14790 24.571 350 225 12 12 351 7 327 9.48e-19 88.2
MS.gene95060.t1 AT3G62830 27.879 330 193 14 14 336 121 412 1.23e-17 84.3
MS.gene95060.t1 AT3G62830 27.879 330 193 14 14 336 121 412 1.23e-17 84.3
MS.gene95060.t1 AT3G53520 27.809 356 208 16 10 354 118 435 1.80e-17 83.6
MS.gene95060.t1 AT2G47650 27.576 330 194 15 14 336 123 414 3.96e-17 82.8
MS.gene95060.t1 AT1G78570 24.786 351 223 14 12 351 7 327 6.55e-17 82.4
MS.gene95060.t1 AT3G53520 27.528 356 207 16 10 354 118 433 9.58e-16 78.6
MS.gene95060.t1 AT2G47650 27.083 336 194 16 14 336 123 420 4.81e-15 76.6
MS.gene95060.t1 AT4G12250 25.581 344 226 11 13 344 97 422 1.59e-14 74.7
MS.gene95060.t1 AT4G30440 25.205 365 223 14 2 344 78 414 2.77e-14 73.9
MS.gene95060.t1 AT3G53520 27.473 364 182 20 10 336 118 436 7.22e-14 72.8
MS.gene95060.t1 AT3G53520 30.159 252 137 13 10 254 118 337 5.50e-11 63.5

Find 121 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTCTGCAGCAGTTTA+TGG 0.176720 8.2:-341998 MS.gene95060:CDS
TTGCCCGAGCTCAACGTTTA+TGG 0.230700 8.2:-341658 MS.gene95060:CDS
TAAGTAAGGCATCAGATTAT+TGG 0.264960 8.2:+341702 None:intergenic
CATAAGGATTCATGGCTTGC+AGG 0.302019 8.2:+341939 None:intergenic
GCTGCATTTGAAAAGGCTTC+TGG 0.308975 8.2:-341299 MS.gene95060:CDS
GGTTCTTCCATAAGGATTCA+TGG 0.337426 8.2:+341931 None:intergenic
TACCAGCTTGAGCTTGGGTT+TGG 0.340617 8.2:-340898 MS.gene95060:CDS
AAGTAAGGCATCAGATTATT+GGG 0.352747 8.2:+341703 None:intergenic
GGTCGTTCAGCTTCTAAAAC+AGG 0.355343 8.2:-342326 MS.gene95060:CDS
CTGCAAGCCATGAATCCTTA+TGG 0.360608 8.2:-341938 MS.gene95060:CDS
TGTCCACAGGGTTCGCCATT+TGG 0.362347 8.2:-342254 MS.gene95060:CDS
GTCGGAAAGGAAAACGATAA+TGG 0.375088 8.2:-342383 MS.gene95060:CDS
ACCAGCTTGAGCTTGGGTTT+GGG 0.376831 8.2:-340897 MS.gene95060:CDS
TGTGCAGAGACCAATGGAAT+TGG 0.380858 8.2:-340942 MS.gene95060:CDS
GCAATCTTCCAACTGCTACT+TGG 0.382864 8.2:+341677 None:intergenic
ACGGGTGGGGCGGGGTTCAT+TGG 0.384071 8.2:-342358 MS.gene95060:CDS
ACTCACCGCAGTGCCATCTT+TGG 0.403250 8.2:+341621 None:intergenic
AGTACCTTGGTTCTTCCATA+AGG 0.405042 8.2:+341923 None:intergenic
TGGGGATACCAGCTTGAGCT+TGG 0.405772 8.2:-340904 MS.gene95060:CDS
ATTGTAGAGATTGTCGATAA+TGG 0.413237 8.2:+342291 None:intergenic
TAATGGTAACGGGTGGGGCG+GGG 0.417054 8.2:-342366 MS.gene95060:CDS
CAATCTTCCAACTGCTACTT+GGG 0.419596 8.2:+341678 None:intergenic
GGGGATACCAGCTTGAGCTT+GGG 0.426150 8.2:-340903 MS.gene95060:CDS
TGAGATACTTCAATCCGGTT+GGG 0.434754 8.2:-341768 MS.gene95060:CDS
GCACATGAGAGCGGTGAGAT+TGG 0.438760 8.2:-341745 MS.gene95060:CDS
TGTTGCTTGCCCAATTCCAT+TGG 0.442181 8.2:+340932 None:intergenic
CCGCTCTCATGTGCCCCAAC+CGG 0.449340 8.2:+341754 None:intergenic
GATAATGGTAACGGGTGGGG+CGG 0.450585 8.2:-342368 MS.gene95060:CDS
TCAACGCAGGGAATTTCATC+TGG 0.454641 8.2:+341971 None:intergenic
CACCGCAGTGCCATCTTTGG+TGG 0.455579 8.2:+341624 None:intergenic
ATAATGGTAACGGGTGGGGC+GGG 0.455901 8.2:-342367 MS.gene95060:CDS
CACAGGGTTCGCCATTTGGT+TGG 0.459853 8.2:-342250 MS.gene95060:CDS
AATGGTTGCTGCATTTGAAA+AGG 0.465118 8.2:-341306 MS.gene95060:CDS
TACAACTTGGGAACTGGCCG+CGG 0.465961 8.2:-341344 MS.gene95060:CDS
ATAGATAGCAGTAGCATCTC+CGG 0.466954 8.2:+341133 None:intergenic
AGATTATTGGGTATGCCCCT+TGG 0.469183 8.2:+341715 None:intergenic
GTGCAGAGACCAATGGAATT+GGG 0.470949 8.2:-340941 MS.gene95060:CDS
AGTGCCTACAACTTGGGAAC+TGG 0.472400 8.2:-341350 MS.gene95060:CDS
TACTTAACCCAAGTAGCAGT+TGG 0.476959 8.2:-341685 MS.gene95060:CDS
AACGATAATGGTAACGGGTG+GGG 0.478155 8.2:-342371 MS.gene95060:CDS
CATAAGGCTGAAAATGAACT+TGG 0.482154 8.2:-341101 MS.gene95060:CDS
CATGATTATCCCACCAAAGA+TGG 0.483974 8.2:-341634 MS.gene95060:CDS
CATCTGCCAGGTCCATTACA+TGG 0.489946 8.2:+341506 None:intergenic
GGTTGTAGTGCCTACAACTT+GGG 0.490332 8.2:-341356 MS.gene95060:CDS
ATTGGGCAAGCAACAATCCA+TGG 0.492922 8.2:-340924 MS.gene95060:CDS
CCGTCATGGAAGCTGTCCAC+AGG 0.495003 8.2:-342267 MS.gene95060:CDS
CGGTACATCAGTGCTTCAAA+TGG 0.497630 8.2:-341324 MS.gene95060:CDS
CTGAGATACTTCAATCCGGT+TGG 0.503362 8.2:-341769 MS.gene95060:CDS
TATCTATGCATCTACGCATA+AGG 0.505409 8.2:-341117 MS.gene95060:CDS
GTGAGATTGGCGAAGATCCA+AGG 0.505565 8.2:-341732 MS.gene95060:CDS
GTGGATCACAGCGTCAAACT+TGG 0.505767 8.2:+342147 None:intergenic
AGGCTGAAAATGAACTTGGT+TGG 0.510946 8.2:-341097 MS.gene95060:CDS
TGACCATAAACGTTGAGCTC+GGG 0.516024 8.2:+341655 None:intergenic
GTAGCATCTCCGGGCCTCCT+CGG 0.516501 8.2:+341143 None:intergenic
ACGGGACTTTATCCATGTAA+TGG 0.518510 8.2:-341518 MS.gene95060:CDS
AGGTTGTAGTGCCTACAACT+TGG 0.522897 8.2:-341357 MS.gene95060:CDS
GCGGCCAGTTCCCAAGTTGT+AGG 0.524177 8.2:+341346 None:intergenic
CAGCAATGTGACCATCTGCC+AGG 0.524516 8.2:+341494 None:intergenic
CTCTGCGACACTTTCTCCCA+CGG 0.527424 8.2:+342108 None:intergenic
ACTGCTACTTGGGTTAAGTA+AGG 0.528316 8.2:+341688 None:intergenic
GAGATACTTCAATCCGGTTG+GGG 0.536252 8.2:-341767 MS.gene95060:CDS
AAAATGTGTCCGAGGAGGCC+CGG 0.536292 8.2:-341152 MS.gene95060:CDS
GACGCTGTGATCCACTTTGG+TGG 0.540445 8.2:-342139 MS.gene95060:CDS
AGGAAAACGATAATGGTAAC+GGG 0.546669 8.2:-342376 MS.gene95060:CDS
TTGATTGACAGGGCAAAATA+CGG 0.547401 8.2:-340976 MS.gene95060:intron
ATGGTGAAAGCGGAGGTAGT+TGG 0.547626 8.2:+342216 None:intergenic
TCTCCAACTCTTGCTTGTTG+CGG 0.553361 8.2:+342185 None:intergenic
ATTGGAAAGAGATGGTGAGT+GGG 0.558504 8.2:-342417 None:intergenic
AAAGTTGAGGACCAACCAAA+TGG 0.558547 8.2:+342239 None:intergenic
GGCAATACCAATCGTCGGAA+AGG 0.560434 8.2:-342396 MS.gene95060:CDS
CTTTGGTGGTCTGAAAGCCG+TGG 0.561091 8.2:-342125 MS.gene95060:CDS
TGAGATTGGCGAAGATCCAA+GGG 0.561571 8.2:-341731 MS.gene95060:CDS
TCTCCGCAACAAGCAAGAGT+TGG 0.561707 8.2:-342188 MS.gene95060:CDS
TTGGGCAAGCAACAATCCAT+GGG 0.563515 8.2:-340923 MS.gene95060:CDS
CAGGGCAAAATACGGTATAG+AGG 0.565533 8.2:-340968 MS.gene95060:CDS
ATTTGAAAAGGCTTCTGGCA+AGG 0.566930 8.2:-341294 MS.gene95060:intron
TCCCAAACCCAAGCTCAAGC+TGG 0.567837 8.2:+340896 None:intergenic
ATTGCTGAGATACTTCAATC+CGG 0.576619 8.2:-341773 MS.gene95060:CDS
ATGACCATAAACGTTGAGCT+CGG 0.577684 8.2:+341654 None:intergenic
GATTGCACGAGATATTCAAA+AGG 0.578799 8.2:-341818 MS.gene95060:CDS
CTTTATCCATGTAATGGACC+TGG 0.579239 8.2:-341512 MS.gene95060:CDS
CATGTAATGGACCTGGCAGA+TGG 0.583826 8.2:-341505 MS.gene95060:CDS
AGCAATATTATCCTCCACTC+TGG 0.589937 8.2:+341790 None:intergenic
TTCAAAAGGCAGAGCCAGAG+TGG 0.592430 8.2:-341804 MS.gene95060:CDS
AGCTCAAGCTGGTATCCCCA+TGG 0.594214 8.2:+340907 None:intergenic
TTTGACGCTGTGATCCACTT+TGG 0.595864 8.2:-342142 MS.gene95060:CDS
CGGAGGTAGTTGGAAAGTTG+AGG 0.595990 8.2:+342226 None:intergenic
TAGATAGCAGTAGCATCTCC+GGG 0.603256 8.2:+341134 None:intergenic
AAACGATAATGGTAACGGGT+GGG 0.605066 8.2:-342372 MS.gene95060:CDS
CAGGTGGAAGTCCTCAACGC+AGG 0.605927 8.2:+341958 None:intergenic
GATTGGAAAGAGATGGTGAG+TGG 0.608418 8.2:-342418 None:intergenic
AGGAGATGTGCAGAGACCAA+TGG 0.609293 8.2:-340948 MS.gene95060:CDS
ACCGCAGTGCCATCTTTGGT+GGG 0.615427 8.2:+341625 None:intergenic
AAGGAAAACGATAATGGTAA+CGG 0.616022 8.2:-342377 MS.gene95060:CDS
CGTCATGGAAGCTGTCCACA+GGG 0.616430 8.2:-342266 MS.gene95060:CDS
AAGGATTCATGGCTTGCAGG+TGG 0.618135 8.2:+341942 None:intergenic
CGGCTTTCAGACCACCAAAG+TGG 0.621055 8.2:+342128 None:intergenic
TTGTGATCAAAATATTTGAG+TGG 0.621926 8.2:+342085 None:intergenic
TTTGGTGGTCTGAAAGCCGT+GGG 0.624204 8.2:-342124 MS.gene95060:CDS
AGGTGGAAGTCCTCAACGCA+GGG 0.631756 8.2:+341959 None:intergenic
AGATGAAATTCCCTGCGTTG+AGG 0.633891 8.2:-341969 MS.gene95060:CDS
CAATCTCTACAATTCCGTCA+TGG 0.635522 8.2:-342281 MS.gene95060:CDS
AAAACGATAATGGTAACGGG+TGG 0.639831 8.2:-342373 MS.gene95060:CDS
CCGGTTGGGGCACATGAGAG+CGG 0.640769 8.2:-341754 MS.gene95060:CDS
TTCCTATTAAAATGTGTCCG+AGG 0.642928 8.2:-341160 MS.gene95060:CDS
GAGTGGGCAATACCAATCGT+CGG 0.643532 8.2:-342401 MS.gene95060:CDS
GTCGTTCAGCTTCTAAAACA+GGG 0.648315 8.2:-342325 MS.gene95060:CDS
AAAAGGCAGAGCCAGAGTGG+AGG 0.653042 8.2:-341801 MS.gene95060:CDS
GGAGATCGACATGGTGAAAG+CGG 0.655928 8.2:+342206 None:intergenic
CTATTAAAATGTGTCCGAGG+AGG 0.658139 8.2:-341157 MS.gene95060:CDS
GCTTGTTGCGGAGATCGACA+TGG 0.660195 8.2:+342197 None:intergenic
TTGACCATAAACTGCTGCAG+AGG 0.664691 8.2:+341994 None:intergenic
CAACCAAATGGCGAACCCTG+TGG 0.665060 8.2:+342251 None:intergenic
GATCGACATGGTGAAAGCGG+AGG 0.669740 8.2:+342209 None:intergenic
CCTGTGGACAGCTTCCATGA+CGG 0.679733 8.2:+342267 None:intergenic
GAATCCTTATGGAAGAACCA+AGG 0.701863 8.2:-341927 MS.gene95060:intron
GGGGTTCATTGGATCACACA+CGG 0.730801 8.2:-342347 MS.gene95060:CDS
TTTGAAGCACTGATGTACCG+CGG 0.744540 8.2:+341327 None:intergenic
TGGGCAAGCAACAATCCATG+GGG 0.753069 8.2:-340922 MS.gene95060:CDS
GAGATTGGCGAAGATCCAAG+GGG 0.771146 8.2:-341730 MS.gene95060:CDS
TCCCACCAAAGATGGCACTG+CGG 0.774633 8.2:-341626 MS.gene95060:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAATACTGTTATTATT+AGG - chr8.2:341917-341936 MS.gene95060:intron 10.0%
!!! ATCATTATTCATTATTTTAA+TGG + chr8.2:341412-341431 None:intergenic 10.0%
!! AGAATACAATACAATACATT+CGG + chr8.2:342071-342090 None:intergenic 20.0%
!! AGTACTTTACTATATTTCAT+TGG - chr8.2:342225-342244 MS.gene95060:CDS 20.0%
!! ATAAACAGTTAAATGTAAGT+AGG + chr8.2:341866-341885 None:intergenic 20.0%
!! GTACTTTACTATATTTCATT+GGG - chr8.2:342226-342245 MS.gene95060:CDS 20.0%
!! TAAATTTCAGTGTAAAAAGA+TGG - chr8.2:341268-341287 MS.gene95060:intron 20.0%
!!! ACATTTTAATAGGAATTTTC+TGG + chr8.2:342129-342148 None:intergenic 20.0%
! AACAGTATTTAATTAAGTCC+TGG + chr8.2:341911-341930 None:intergenic 25.0%
! ACAATATATGAATCAGATAC+GGG - chr8.2:341758-341777 MS.gene95060:CDS 25.0%
! ATGAATAATGATACTTAAGC+TGG - chr8.2:341420-341439 MS.gene95060:intron 25.0%
! GACAATATATGAATCAGATA+CGG - chr8.2:341757-341776 MS.gene95060:CDS 25.0%
! TCAGTATCAGAATATATGTT+GGG + chr8.2:341739-341758 None:intergenic 25.0%
! TTATTTACGATTGATTGACA+GGG - chr8.2:342308-342327 MS.gene95060:CDS 25.0%
! TTGTGATCAAAATATTTGAG+TGG + chr8.2:341212-341231 None:intergenic 25.0%
! TTTATTTACGATTGATTGAC+AGG - chr8.2:342307-342326 MS.gene95060:CDS 25.0%
!!! TATTTTGATCACAACTTGAT+TGG - chr8.2:341218-341237 MS.gene95060:intron 25.0%
AGTCATCACTTGAAAAAGAT+TGG + chr8.2:341249-341268 None:intergenic 30.0%
ATATACTGAGTTAAGTACCT+TGG + chr8.2:341387-341406 None:intergenic 30.0%
ATTGTAGAGATTGTCGATAA+TGG + chr8.2:341006-341025 None:intergenic 30.0%
CTTTACTATATTTCATTGGG+AGG - chr8.2:342229-342248 MS.gene95060:CDS 30.0%
GTCAGTATCAGAATATATGT+TGG + chr8.2:341740-341759 None:intergenic 30.0%
! AAGGAAAACGATAATGGTAA+CGG - chr8.2:340917-340936 MS.gene95060:CDS 30.0%
! AAGTAAGGCATCAGATTATT+GGG + chr8.2:341594-341613 None:intergenic 30.0%
! TAAGTAAGGCATCAGATTAT+TGG + chr8.2:341595-341614 None:intergenic 30.0%
AATATATGTTGGGTGCTAAC+TGG + chr8.2:341729-341748 None:intergenic 35.0%
AATGGTTGCTGCATTTGAAA+AGG - chr8.2:341988-342007 MS.gene95060:CDS 35.0%
ACTATATTTCATTGGGAGGA+AGG - chr8.2:342233-342252 MS.gene95060:CDS 35.0%
ATTGCTGAGATACTTCAATC+CGG - chr8.2:341521-341540 MS.gene95060:CDS 35.0%
ATTGTAGATTAGAGCTAACC+AGG - chr8.2:341890-341909 MS.gene95060:intron 35.0%
CATAAGGCTGAAAATGAACT+TGG - chr8.2:342193-342212 MS.gene95060:CDS 35.0%
CTATATTTCATTGGGAGGAA+GGG - chr8.2:342234-342253 MS.gene95060:CDS 35.0%
GATTGCACGAGATATTCAAA+AGG - chr8.2:341476-341495 MS.gene95060:CDS 35.0%
TATCTATGCATCTACGCATA+AGG - chr8.2:342177-342196 MS.gene95060:CDS 35.0%
TGTATTCTTGATCCTTCGTT+TGG - chr8.2:342083-342102 MS.gene95060:CDS 35.0%
TTCCTATTAAAATGTGTCCG+AGG - chr8.2:342134-342153 MS.gene95060:CDS 35.0%
TTGATTGACAGGGCAAAATA+CGG - chr8.2:342318-342337 MS.gene95060:CDS 35.0%
! AGGAAAACGATAATGGTAAC+GGG - chr8.2:340918-340937 MS.gene95060:CDS 35.0%
! CTTTTTGCGACAGACAAAAT+CGG - chr8.2:341834-341853 MS.gene95060:CDS 35.0%
! TGATATATGACAGCTTTTCG+CGG - chr8.2:341449-341468 MS.gene95060:intron 35.0%
AAAGTTGAGGACCAACCAAA+TGG + chr8.2:341058-341077 None:intergenic 40.0%
ACGGGACTTTATCCATGTAA+TGG - chr8.2:341776-341795 MS.gene95060:CDS 40.0%
AGCAATATTATCCTCCACTC+TGG + chr8.2:341507-341526 None:intergenic 40.0%
AGGCTGAAAATGAACTTGGT+TGG - chr8.2:342197-342216 MS.gene95060:CDS 40.0%
ATAGATAGCAGTAGCATCTC+CGG + chr8.2:342164-342183 None:intergenic 40.0%
ATGACCATAAACGTTGAGCT+CGG + chr8.2:341643-341662 None:intergenic 40.0%
ATTTGAAAAGGCTTCTGGCA+AGG - chr8.2:342000-342019 MS.gene95060:CDS 40.0%
CAATCTCTACAATTCCGTCA+TGG - chr8.2:341013-341032 MS.gene95060:intron 40.0%
CATGATTATCCCACCAAAGA+TGG - chr8.2:341660-341679 MS.gene95060:CDS 40.0%
CTATTAAAATGTGTCCGAGG+AGG - chr8.2:342137-342156 MS.gene95060:CDS 40.0%
GAATCCTTATGGAAGAACCA+AGG - chr8.2:341367-341386 MS.gene95060:intron 40.0%
GGTTCTTCCATAAGGATTCA+TGG + chr8.2:341366-341385 None:intergenic 40.0%
GTCGGAAAGGAAAACGATAA+TGG - chr8.2:340911-340930 MS.gene95060:CDS 40.0%
TACTTAACCCAAGTAGCAGT+TGG - chr8.2:341609-341628 MS.gene95060:intron 40.0%
TCTGGAAAACAGATCCAAGA+AGG + chr8.2:342111-342130 None:intergenic 40.0%
TGAGATACTTCAATCCGGTT+GGG - chr8.2:341526-341545 MS.gene95060:intron 40.0%
! ACTGCTACTTGGGTTAAGTA+AGG + chr8.2:341609-341628 None:intergenic 40.0%
! AGTACCTTGGTTCTTCCATA+AGG + chr8.2:341374-341393 None:intergenic 40.0%
! CAATCTTCCAACTGCTACTT+GGG + chr8.2:341619-341638 None:intergenic 40.0%
!! AAAACGATAATGGTAACGGG+TGG - chr8.2:340921-340940 MS.gene95060:CDS 40.0%
!! AAACGATAATGGTAACGGGT+GGG - chr8.2:340922-340941 MS.gene95060:CDS 40.0%
!! CTCCTCGGACACATTTTAAT+AGG + chr8.2:342139-342158 None:intergenic 40.0%
!! CTTTATCCATGTAATGGACC+TGG - chr8.2:341782-341801 MS.gene95060:CDS 40.0%
!! GTCGTTCAGCTTCTAAAACA+GGG - chr8.2:340969-340988 MS.gene95060:intron 40.0%
AGATGAAATTCCCTGCGTTG+AGG - chr8.2:341325-341344 MS.gene95060:CDS 45.0%
AGATTATTGGGTATGCCCCT+TGG + chr8.2:341582-341601 None:intergenic 45.0%
AGGTTGTAGTGCCTACAACT+TGG - chr8.2:341937-341956 MS.gene95060:CDS 45.0%
ATTGGGCAAGCAACAATCCA+TGG - chr8.2:342370-342389 MS.gene95060:CDS 45.0%
CAGGGCAAAATACGGTATAG+AGG - chr8.2:342326-342345 MS.gene95060:CDS 45.0%
CGGTACATCAGTGCTTCAAA+TGG - chr8.2:341970-341989 MS.gene95060:CDS 45.0%
CTGAGATACTTCAATCCGGT+TGG - chr8.2:341525-341544 MS.gene95060:intron 45.0%
CTGCAAGCCATGAATCCTTA+TGG - chr8.2:341356-341375 MS.gene95060:CDS 45.0%
GAGATACTTCAATCCGGTTG+GGG - chr8.2:341527-341546 MS.gene95060:intron 45.0%
GCAATCTTCCAACTGCTACT+TGG + chr8.2:341620-341639 None:intergenic 45.0%
GCTGCATTTGAAAAGGCTTC+TGG - chr8.2:341995-342014 MS.gene95060:CDS 45.0%
GGTTGTAGTGCCTACAACTT+GGG - chr8.2:341938-341957 MS.gene95060:CDS 45.0%
GTGCAGAGACCAATGGAATT+GGG - chr8.2:342353-342372 MS.gene95060:CDS 45.0%
TAGATAGCAGTAGCATCTCC+GGG + chr8.2:342163-342182 None:intergenic 45.0%
TATTTCATTGGGAGGAAGGG+AGG - chr8.2:342237-342256 MS.gene95060:CDS 45.0%
TCAACGCAGGGAATTTCATC+TGG + chr8.2:341326-341345 None:intergenic 45.0%
TCATCCTCTGCAGCAGTTTA+TGG - chr8.2:341296-341315 MS.gene95060:CDS 45.0%
TGACCATAAACGTTGAGCTC+GGG + chr8.2:341642-341661 None:intergenic 45.0%
TGAGATTGGCGAAGATCCAA+GGG - chr8.2:341563-341582 MS.gene95060:intron 45.0%
TGTGCAGAGACCAATGGAAT+TGG - chr8.2:342352-342371 MS.gene95060:CDS 45.0%
TTGACCATAAACTGCTGCAG+AGG + chr8.2:341303-341322 None:intergenic 45.0%
TTGCGACAGACAAAATCGGT+AGG - chr8.2:341838-341857 MS.gene95060:intron 45.0%
TTGGGCAAGCAACAATCCAT+GGG - chr8.2:342371-342390 MS.gene95060:CDS 45.0%
TTTGACGCTGTGATCCACTT+TGG - chr8.2:341152-341171 MS.gene95060:CDS 45.0%
! CATAAGGATTCATGGCTTGC+AGG + chr8.2:341358-341377 None:intergenic 45.0%
! TCGTTTTCCTTTCCGACGAT+TGG + chr8.2:340908-340927 None:intergenic 45.0%
! TCTCCAACTCTTGCTTGTTG+CGG + chr8.2:341112-341131 None:intergenic 45.0%
! TGTTGCTTGCCCAATTCCAT+TGG + chr8.2:342365-342384 None:intergenic 45.0%
!! AACGATAATGGTAACGGGTG+GGG - chr8.2:340923-340942 MS.gene95060:CDS 45.0%
!! GGTCGTTCAGCTTCTAAAAC+AGG - chr8.2:340968-340987 MS.gene95060:CDS 45.0%
!! TTTGAAGCACTGATGTACCG+CGG + chr8.2:341970-341989 None:intergenic 45.0%
ACCAGCTTGAGCTTGGGTTT+GGG - chr8.2:342397-342416 MS.gene95060:CDS 50.0%
AGGAGATGTGCAGAGACCAA+TGG - chr8.2:342346-342365 MS.gene95060:CDS 50.0%
AGTGCCTACAACTTGGGAAC+TGG - chr8.2:341944-341963 MS.gene95060:CDS 50.0%
ATGGTGAAAGCGGAGGTAGT+TGG + chr8.2:341081-341100 None:intergenic 50.0%
CATCTGCCAGGTCCATTACA+TGG + chr8.2:341791-341810 None:intergenic 50.0%
CGGAGGTAGTTGGAAAGTTG+AGG + chr8.2:341071-341090 None:intergenic 50.0%
GAGATTGGCGAAGATCCAAG+GGG - chr8.2:341564-341583 MS.gene95060:intron 50.0%
GAGTGGGCAATACCAATCGT+CGG - chr8.2:340893-340912 MS.gene95060:CDS 50.0%
GGAGATCGACATGGTGAAAG+CGG + chr8.2:341091-341110 None:intergenic 50.0%
GGCAATACCAATCGTCGGAA+AGG - chr8.2:340898-340917 MS.gene95060:CDS 50.0%
GGGGTTCATTGGATCACACA+CGG - chr8.2:340947-340966 MS.gene95060:CDS 50.0%
GTGAGATTGGCGAAGATCCA+AGG - chr8.2:341562-341581 MS.gene95060:intron 50.0%
GTGGATCACAGCGTCAAACT+TGG + chr8.2:341150-341169 None:intergenic 50.0%
TACCAGCTTGAGCTTGGGTT+TGG - chr8.2:342396-342415 MS.gene95060:CDS 50.0%
TCCAAGAAGGAGCCAAACGA+AGG + chr8.2:342098-342117 None:intergenic 50.0%
TCTCCGCAACAAGCAAGAGT+TGG - chr8.2:341106-341125 MS.gene95060:CDS 50.0%
TGGGCAAGCAACAATCCATG+GGG - chr8.2:342372-342391 MS.gene95060:CDS 50.0%
TTCAAAAGGCAGAGCCAGAG+TGG - chr8.2:341490-341509 MS.gene95060:CDS 50.0%
TTGCCCGAGCTCAACGTTTA+TGG - chr8.2:341636-341655 MS.gene95060:CDS 50.0%
! AAGGATTCATGGCTTGCAGG+TGG + chr8.2:341355-341374 None:intergenic 50.0%
! TCCTTCGTTTGGCTCCTTCT+TGG - chr8.2:342094-342113 MS.gene95060:CDS 50.0%
! TTTGGTGGTCTGAAAGCCGT+GGG - chr8.2:341170-341189 MS.gene95060:intron 50.0%
!! CATGTAATGGACCTGGCAGA+TGG - chr8.2:341789-341808 MS.gene95060:CDS 50.0%
AAAAGGCAGAGCCAGAGTGG+AGG - chr8.2:341493-341512 MS.gene95060:CDS 55.0%
AAAATGTGTCCGAGGAGGCC+CGG - chr8.2:342142-342161 MS.gene95060:CDS 55.0%
ACCGCAGTGCCATCTTTGGT+GGG + chr8.2:341672-341691 None:intergenic 55.0%
ACTCACCGCAGTGCCATCTT+TGG + chr8.2:341676-341695 None:intergenic 55.0%
AGCTCAAGCTGGTATCCCCA+TGG + chr8.2:342390-342409 None:intergenic 55.0%
AGGTGGAAGTCCTCAACGCA+GGG + chr8.2:341338-341357 None:intergenic 55.0%
CAACCAAATGGCGAACCCTG+TGG + chr8.2:341046-341065 None:intergenic 55.0%
CACAGGGTTCGCCATTTGGT+TGG - chr8.2:341044-341063 MS.gene95060:intron 55.0%
CAGCAATGTGACCATCTGCC+AGG + chr8.2:341803-341822 None:intergenic 55.0%
CCTGTGGACAGCTTCCATGA+CGG + chr8.2:341030-341049 None:intergenic 55.0%
CGGCTTTCAGACCACCAAAG+TGG + chr8.2:341169-341188 None:intergenic 55.0%
CGTCATGGAAGCTGTCCACA+GGG - chr8.2:341028-341047 MS.gene95060:intron 55.0%
CTCTGCGACACTTTCTCCCA+CGG + chr8.2:341189-341208 None:intergenic 55.0%
GACGCTGTGATCCACTTTGG+TGG - chr8.2:341155-341174 MS.gene95060:CDS 55.0%
GATCGACATGGTGAAAGCGG+AGG + chr8.2:341088-341107 None:intergenic 55.0%
GCACATGAGAGCGGTGAGAT+TGG - chr8.2:341549-341568 MS.gene95060:intron 55.0%
GGGGATACCAGCTTGAGCTT+GGG - chr8.2:342391-342410 MS.gene95060:CDS 55.0%
TACAACTTGGGAACTGGCCG+CGG - chr8.2:341950-341969 MS.gene95060:CDS 55.0%
TCATTGGGAGGAAGGGAGGA+GGG - chr8.2:342241-342260 MS.gene95060:CDS 55.0%
TCCCAAACCCAAGCTCAAGC+TGG + chr8.2:342401-342420 None:intergenic 55.0%
TCCCACCAAAGATGGCACTG+CGG - chr8.2:341668-341687 MS.gene95060:CDS 55.0%
TGGGGATACCAGCTTGAGCT+TGG - chr8.2:342390-342409 MS.gene95060:CDS 55.0%
TGTCCACAGGGTTCGCCATT+TGG - chr8.2:341040-341059 MS.gene95060:intron 55.0%
TTCATTGGGAGGAAGGGAGG+AGG - chr8.2:342240-342259 MS.gene95060:CDS 55.0%
! CTTTGGTGGTCTGAAAGCCG+TGG - chr8.2:341169-341188 MS.gene95060:intron 55.0%
! GCTTGTTGCGGAGATCGACA+TGG + chr8.2:341100-341119 None:intergenic 55.0%
!! ATAATGGTAACGGGTGGGGC+GGG - chr8.2:340927-340946 MS.gene95060:CDS 55.0%
!! GATAATGGTAACGGGTGGGG+CGG - chr8.2:340926-340945 MS.gene95060:CDS 55.0%
CACCGCAGTGCCATCTTTGG+TGG + chr8.2:341673-341692 None:intergenic 60.0%
CAGGTGGAAGTCCTCAACGC+AGG + chr8.2:341339-341358 None:intergenic 60.0%
CCGTCATGGAAGCTGTCCAC+AGG - chr8.2:341027-341046 MS.gene95060:intron 60.0%
GCGGCCAGTTCCCAAGTTGT+AGG + chr8.2:341951-341970 None:intergenic 60.0%
!! TAATGGTAACGGGTGGGGCG+GGG - chr8.2:340928-340947 MS.gene95060:CDS 60.0%
CCGCTCTCATGTGCCCCAAC+CGG + chr8.2:341543-341562 None:intergenic 65.0%
CCGGTTGGGGCACATGAGAG+CGG - chr8.2:341540-341559 MS.gene95060:intron 65.0%
GTAGCATCTCCGGGCCTCCT+CGG + chr8.2:342154-342173 None:intergenic 65.0%
ACGGGTGGGGCGGGGTTCAT+TGG - chr8.2:340936-340955 MS.gene95060:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 340888 342428 340888 ID=MS.gene95060
chr8.2 mRNA 340888 342428 340888 ID=MS.gene95060.t1;Parent=MS.gene95060
chr8.2 exon 341928 342428 341928 ID=MS.gene95060.t1.exon1;Parent=MS.gene95060.t1
chr8.2 CDS 341928 342428 341928 ID=cds.MS.gene95060.t1;Parent=MS.gene95060.t1
chr8.2 exon 341627 341854 341627 ID=MS.gene95060.t1.exon2;Parent=MS.gene95060.t1
chr8.2 CDS 341627 341854 341627 ID=cds.MS.gene95060.t1;Parent=MS.gene95060.t1
chr8.2 exon 341461 341542 341461 ID=MS.gene95060.t1.exon3;Parent=MS.gene95060.t1
chr8.2 CDS 341461 341542 341461 ID=cds.MS.gene95060.t1;Parent=MS.gene95060.t1
chr8.2 exon 341295 341377 341295 ID=MS.gene95060.t1.exon4;Parent=MS.gene95060.t1
chr8.2 CDS 341295 341377 341295 ID=cds.MS.gene95060.t1;Parent=MS.gene95060.t1
chr8.2 exon 341095 341186 341095 ID=MS.gene95060.t1.exon5;Parent=MS.gene95060.t1
chr8.2 CDS 341095 341186 341095 ID=cds.MS.gene95060.t1;Parent=MS.gene95060.t1
chr8.2 exon 340888 340987 340888 ID=MS.gene95060.t1.exon6;Parent=MS.gene95060.t1
chr8.2 CDS 340888 340987 340888 ID=cds.MS.gene95060.t1;Parent=MS.gene95060.t1
Gene Sequence

>MS.gene95060

ATGGTGAGTGGGCAATACCAATCGTCGGAAAGGAAAACGATAATGGTAACGGGTGGGGCGGGGTTCATTGGATCACACACGGTCGTTCAGCTTCTAAAACAGGGCTATACAGTTTCCATTATCGACAATCTCTACAATTCCGTCATGGAAGCTGTCCACAGGGTTCGCCATTTGGTTGGTCCTCAACTTTCCAACTACCTCCGCTTTCACCATGTCGATCTCCGCAACAAGCAAGAGTTGGAGATGATCTTCTCTAAAACCAAGTTTGACGCTGTGATCCACTTTGGTGGTCTGAAAGCCGTGGGAGAAAGTGTCGCAGAGCCACTCAAATATTTTGATCACAACTTGATTGGCACTACCAATCTTTTTCAAGTGATGACTAAATTTCAGTGTAAAAAGATGGTATTGTCATCCTCTGCAGCAGTTTATGGTCAACCAGATGAAATTCCCTGCGTTGAGGACTTCCACCTGCAAGCCATGAATCCTTATGGAAGAACCAAGGTACTTAACTCAGTATATATCCATTAAAATAATGAATAATGATACTTAAGCTGGCATATATGATATATGACAGCTTTTCGCGGAAGAGATTGCACGAGATATTCAAAAGGCAGAGCCAGAGTGGAGGATAATATTGCTGAGATACTTCAATCCGGTTGGGGCACATGAGAGCGGTGAGATTGGCGAAGATCCAAGGGGCATACCCAATAATCTGATGCCTTACTTAACCCAAGTAGCAGTTGGAAGATTGCCCGAGCTCAACGTTTATGGTCATGATTATCCCACCAAAGATGGCACTGCGGTGAGTGTCTTAATTCACTCATCATATTGATATTTACCAGTTAGCACCCAACATATATTCTGATACTGACAATATATGAATCAGATACGGGACTTTATCCATGTAATGGACCTGGCAGATGGTCACATTGCTGCGTTGCGAAAGCTTTTTGCGACAGACAAAATCGGTAGGTACCTACTTACATTTAACTGTTTATTTGAATTGTAGATTAGAGCTAACCAGGACTTAATTAAATACTGTTATTATTAGGTTGTAGTGCCTACAACTTGGGAACTGGCCGCGGTACATCAGTGCTTCAAATGGTTGCTGCATTTGAAAAGGCTTCTGGCAAGGTCTTTTTTTTTATATAGTCTTCTGTACTGTAGCATGTATGCATCTCCGAATGTATTGTATTGTATTCTTGATCCTTCGTTTGGCTCCTTCTTGGATCTGTTTTCCAGAAAATTCCTATTAAAATGTGTCCGAGGAGGCCCGGAGATGCTACTGCTATCTATGCATCTACGCATAAGGCTGAAAATGAACTTGGTTGGAAGTAAGTACTTTACTATATTTCATTGGGAGGAAGGGAGGAGGGATTATTACATTCTTTTAATTAACTAATAATAAAAAGAGTGTGCTTTATTTACGATTGATTGACAGGGCAAAATACGGTATAGAGGAGATGTGCAGAGACCAATGGAATTGGGCAAGCAACAATCCATGGGGATACCAGCTTGAGCTTGGGTTTGGGAATCGTTGA
Protein sequence

>MS.gene95060.t1

MVSGQYQSSERKTIMVTGGAGFIGSHTVVQLLKQGYTVSIIDNLYNSVMEAVHRVRHLVGPQLSNYLRFHHVDLRNKQELEMIFSKTKFDAVIHFGGLKAVGESVAEPLKYFDHNLIGTTNLFQVMTKFQCKKMVLSSSAAVYGQPDEIPCVEDFHLQAMNPYGRTKLFAEEIARDIQKAEPEWRIILLRYFNPVGAHESGEIGEDPRGIPNNLMPYLTQVAVGRLPELNVYGHDYPTKDGTAIRDFIHVMDLADGHIAALRKLFATDKIGCSAYNLGTGRGTSVLQMVAAFEKASGKKIPIKMCPRRPGDATAIYASTHKAENELGWKAKYGIEEMCRDQWNWASNNPWGYQLELGFGNR