Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95083.t1 | XP_013458668.1 | 90 | 110 | 5 | 3 | 2 | 108 | 39 | 145 | 1.60E-42 | 182.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95083.t1 | G7ZZT9 | 90.0 | 110 | 5 | 3 | 2 | 108 | 39 | 145 | 1.1e-42 | 182.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060412 | MS.gene95083 | 0.817327 | 3.49E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95083.t1 | MTR_4g134140 | 88.182 | 110 | 7 | 3 | 2 | 108 | 39 | 145 | 1.35e-54 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGCACTGAACAGCTTAA+AGG | 0.242225 | 8.2:+606534 | None:intergenic |
| CAAGTGTTTGATTACAATTT+CGG | 0.312597 | 8.2:-607552 | MS.gene95083:CDS |
| TTTGAAGAAGGAAAGTAGAT+TGG | 0.355768 | 8.2:-607487 | MS.gene95083:CDS |
| CAAGCATGGAAGCACTTTCA+TGG | 0.384069 | 8.2:-607344 | MS.gene95083:CDS |
| TTGGAATTAGTTTGACTTGA+GGG | 0.414606 | 8.2:+607265 | None:intergenic |
| CTGGTCCATGTAGCGGCTGC+TGG | 0.430691 | 8.2:-606501 | MS.gene95083:CDS |
| AGAGAAACAGTAGTTGTAGA+AGG | 0.431084 | 8.2:+607300 | None:intergenic |
| GTTTGAGATAAATTTGAAGA+AGG | 0.464932 | 8.2:-607499 | MS.gene95083:CDS |
| AGTGCTGTGCTGCTATCTGC+TGG | 0.472010 | 8.2:-606520 | MS.gene95083:CDS |
| AGGTGTCACGGCATGTTTCA+AGG | 0.477649 | 8.2:+607320 | None:intergenic |
| TATAGATGATGTTGTTTATG+TGG | 0.505857 | 8.2:-607391 | MS.gene95083:CDS |
| GAGAGCAACTTACATGGAGT+TGG | 0.508185 | 8.2:+607246 | None:intergenic |
| ATGGACCAGCAGCCGCTACA+TGG | 0.511356 | 8.2:+606496 | None:intergenic |
| AATTTCGGAGACGACGATGA+TGG | 0.516786 | 8.2:-607537 | MS.gene95083:CDS |
| CGAAATTGTAATCAAACACT+TGG | 0.523720 | 8.2:+607553 | None:intergenic |
| ATTGGATTCAATAAGAGAAG+AGG | 0.530259 | 8.2:-607469 | MS.gene95083:CDS |
| TATGTGGCAGTGGGAACAAA+TGG | 0.556548 | 8.2:-607375 | MS.gene95083:CDS |
| TGATGTTGTTTATGTGGCAG+TGG | 0.557388 | 8.2:-607385 | MS.gene95083:CDS |
| AAAAGCAACACAGAACTACA+TGG | 0.570086 | 8.2:+606477 | None:intergenic |
| GTTGGAATTAGTTTGACTTG+AGG | 0.589120 | 8.2:+607264 | None:intergenic |
| TGACTTGAGGGAAAACGTGT+AGG | 0.604542 | 8.2:+607277 | None:intergenic |
| AAATGGTGGAAGTTCAAGCA+TGG | 0.604604 | 8.2:-607358 | MS.gene95083:CDS |
| CTATCTGCTGGTCCATGTAG+CGG | 0.639553 | 8.2:-606508 | MS.gene95083:CDS |
| GATGTTGTTTATGTGGCAGT+GGG | 0.642705 | 8.2:-607384 | MS.gene95083:CDS |
| AGTAGTTGTAGAAGGTGTCA+CGG | 0.650946 | 8.2:+607308 | None:intergenic |
| ATTCAATAAGAGAAGAGGAG+TGG | 0.652515 | 8.2:-607464 | MS.gene95083:CDS |
| GTGGCAGTGGGAACAAATGG+TGG | 0.662195 | 8.2:-607372 | MS.gene95083:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTAGATAAAGTTAAATT+TGG | + | chr8.2:606897-606916 | None:intergenic | 15.0% |
| !!! | CAAAAATCATTTTGATACAT+TGG | - | chr8.2:607259-607278 | MS.gene95083:CDS | 20.0% |
| ! | CAAGTGTTTGATTACAATTT+CGG | - | chr8.2:606501-606520 | MS.gene95083:CDS | 25.0% |
| ! | TATGATGTGCAATTAATAAG+AGG | + | chr8.2:606937-606956 | None:intergenic | 25.0% |
| ! | TGTTTGTAAATAAGACAAGA+AGG | + | chr8.2:606832-606851 | None:intergenic | 25.0% |
| !! | GTTTGAGATAAATTTGAAGA+AGG | - | chr8.2:606554-606573 | MS.gene95083:intron | 25.0% |
| !! | TATAGATGATGTTGTTTATG+TGG | - | chr8.2:606662-606681 | MS.gene95083:intron | 25.0% |
| AGCGTGTAATGTATTAAACA+AGG | - | chr8.2:607207-607226 | MS.gene95083:intron | 30.0% | |
| ATGGTTTCTCGCTAAAATTA+AGG | - | chr8.2:607038-607057 | MS.gene95083:intron | 30.0% | |
| ATTGGATTCAATAAGAGAAG+AGG | - | chr8.2:606584-606603 | MS.gene95083:intron | 30.0% | |
| CGAAATTGTAATCAAACACT+TGG | + | chr8.2:606503-606522 | None:intergenic | 30.0% | |
| GCAGAACTATATGTATTCAA+TGG | + | chr8.2:607454-607473 | None:intergenic | 30.0% | |
| TTTGAAGAAGGAAAGTAGAT+TGG | - | chr8.2:606566-606585 | MS.gene95083:intron | 30.0% | |
| ! | ATTTTCCTCGGTATTTAACT+TGG | + | chr8.2:607146-607165 | None:intergenic | 30.0% |
| ! | TTGGTGATTTATGCTTATGT+TGG | + | chr8.2:606878-606897 | None:intergenic | 30.0% |
| !! | TTGGAATTAGTTTGACTTGA+GGG | + | chr8.2:606791-606810 | None:intergenic | 30.0% |
| AGAGAAACAGTAGTTGTAGA+AGG | + | chr8.2:606756-606775 | None:intergenic | 35.0% | |
| ATTCAATAAGAGAAGAGGAG+TGG | - | chr8.2:606589-606608 | MS.gene95083:intron | 35.0% | |
| CAAAAATTTCCGAGGAACAA+TGG | - | chr8.2:607019-607038 | MS.gene95083:intron | 35.0% | |
| GAAGGAAAATTGACAGAGTT+TGG | + | chr8.2:607371-607390 | None:intergenic | 35.0% | |
| GCTAAAATTAAGGATTTCCG+AGG | - | chr8.2:607048-607067 | MS.gene95083:intron | 35.0% | |
| GTGATTTATGCTTATGTTGG+AGG | + | chr8.2:606875-606894 | None:intergenic | 35.0% | |
| ! | CATTGTTCCTCGGAAATTTT+TGG | + | chr8.2:607021-607040 | None:intergenic | 35.0% |
| ! | CTTTTCCAAGTTAAATACCG+AGG | - | chr8.2:607138-607157 | MS.gene95083:intron | 35.0% |
| ! | TAATGACAACATGTTTTCCG+AGG | + | chr8.2:607172-607191 | None:intergenic | 35.0% |
| !! | GTTGGAATTAGTTTGACTTG+AGG | + | chr8.2:606792-606811 | None:intergenic | 35.0% |
| AAGAAGGAGAGCAACTTACA+TGG | + | chr8.2:606816-606835 | None:intergenic | 40.0% | |
| ATACCGAGGAAAATGTTCCT+CGG | - | chr8.2:607152-607171 | MS.gene95083:intron | 40.0% | |
| ATTCCGACCAAAAATTTCCG+AGG | - | chr8.2:607011-607030 | MS.gene95083:intron | 40.0% | |
| ATTCTGACATGTACATACCG+AGG | + | chr8.2:607082-607101 | None:intergenic | 40.0% | |
| ! | AAATGGTGGAAGTTCAAGCA+TGG | - | chr8.2:606695-606714 | MS.gene95083:intron | 40.0% |
| ! | ATTCGGTGAGAGTTCTTCAA+CGG | + | chr8.2:607397-607416 | None:intergenic | 40.0% |
| ! | CTGTTTGCACCTTCTGTTTT+TGG | - | chr8.2:607485-607504 | MS.gene95083:CDS | 40.0% |
| ! | GATGTTGTTTATGTGGCAGT+GGG | - | chr8.2:606669-606688 | MS.gene95083:intron | 40.0% |
| ! | TGATGTTGTTTATGTGGCAG+TGG | - | chr8.2:606668-606687 | MS.gene95083:intron | 40.0% |
| ! | TTTCCGAGGAACATTTTCCT+CGG | + | chr8.2:607158-607177 | None:intergenic | 40.0% |
| !! | AGTAGTTGTAGAAGGTGTCA+CGG | + | chr8.2:606748-606767 | None:intergenic | 40.0% |
| !!! | GTTCCTCGGAAATTTTTGGT+CGG | + | chr8.2:607017-607036 | None:intergenic | 40.0% |
| AATTTCGGAGACGACGATGA+TGG | - | chr8.2:606516-606535 | MS.gene95083:CDS | 45.0% | |
| AGAGTTCTTCAACGGTGAGA+AGG | + | chr8.2:607389-607408 | None:intergenic | 45.0% | |
| AGCGAGAAACCATTGTTCCT+CGG | + | chr8.2:607031-607050 | None:intergenic | 45.0% | |
| ATACCGAGGAAAGAGTTCCT+CGG | + | chr8.2:607068-607087 | None:intergenic | 45.0% | |
| GAGAGCAACTTACATGGAGT+TGG | + | chr8.2:606810-606829 | None:intergenic | 45.0% | |
| TATGTGGCAGTGGGAACAAA+TGG | - | chr8.2:606678-606697 | MS.gene95083:intron | 45.0% | |
| TGACTTGAGGGAAAACGTGT+AGG | + | chr8.2:606779-606798 | None:intergenic | 45.0% | |
| TTTCCGAGGAACTCTTTCCT+CGG | - | chr8.2:607062-607081 | MS.gene95083:intron | 45.0% | |
| ! | CACAGCACTGAACAGCTTAA+AGG | + | chr8.2:607522-607541 | None:intergenic | 45.0% |
| !! | CAAGCATGGAAGCACTTTCA+TGG | - | chr8.2:606709-606728 | MS.gene95083:intron | 45.0% |
| !!! | TGCACCTTCTGTTTTTGGTC+TGG | - | chr8.2:607490-607509 | MS.gene95083:CDS | 45.0% |
| AGAGAGAGAGAGAGAGAGAG+AGG | + | chr8.2:607293-607312 | None:intergenic | 50.0% | |
| ATGGTTGTGTGACCCAGCTT+CGG | + | chr8.2:607435-607454 | None:intergenic | 50.0% | |
| CCTGCCAGACCAAAAACAGA+AGG | + | chr8.2:607497-607516 | None:intergenic | 50.0% | |
| CTATCTGCTGGTCCATGTAG+CGG | - | chr8.2:607545-607564 | MS.gene95083:CDS | 50.0% | |
| CTCTCTCTCTCTCTCTCAGA+AGG | - | chr8.2:607313-607332 | MS.gene95083:CDS | 50.0% | |
| GGTTACGGTAAGGGAGGATT+CGG | + | chr8.2:607414-607433 | None:intergenic | 50.0% | |
| ! | AGGTGTCACGGCATGTTTCA+AGG | + | chr8.2:606736-606755 | None:intergenic | 50.0% |
| !!! | CCTTCTGTTTTTGGTCTGGC+AGG | - | chr8.2:607494-607513 | MS.gene95083:CDS | 50.0% |
| ACCCAGCTTCGGTTACGGTA+AGG | + | chr8.2:607424-607443 | None:intergenic | 55.0% | |
| AGCTTCGGTTACGGTAAGGG+AGG | + | chr8.2:607420-607439 | None:intergenic | 55.0% | |
| CACGCAGTGCAAACTCAGTG+CGG | - | chr8.2:607111-607130 | MS.gene95083:intron | 55.0% | |
| CCCAGCTTCGGTTACGGTAA+GGG | + | chr8.2:607423-607442 | None:intergenic | 55.0% | |
| CCCTTACCGTAACCGAAGCT+GGG | - | chr8.2:607420-607439 | MS.gene95083:CDS | 55.0% | |
| GTGGCAGTGGGAACAAATGG+TGG | - | chr8.2:606681-606700 | MS.gene95083:intron | 55.0% | |
| GTGTGACCCAGCTTCGGTTA+CGG | + | chr8.2:607429-607448 | None:intergenic | 55.0% | |
| TCCCTTACCGTAACCGAAGC+TGG | - | chr8.2:607419-607438 | MS.gene95083:CDS | 55.0% | |
| ! | AGTGCTGTGCTGCTATCTGC+TGG | - | chr8.2:607533-607552 | MS.gene95083:CDS | 55.0% |
| ATGGACCAGCAGCCGCTACA+TGG | + | chr8.2:607560-607579 | None:intergenic | 60.0% | |
| CGCACTGAGTTTGCACTGCG+TGG | + | chr8.2:607113-607132 | None:intergenic | 60.0% | |
| CTGGTCCATGTAGCGGCTGC+TGG | - | chr8.2:607552-607571 | MS.gene95083:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 606492 | 607583 | 606492 | ID=MS.gene95083 |
| chr8.2 | mRNA | 606492 | 607583 | 606492 | ID=MS.gene95083.t1;Parent=MS.gene95083 |
| chr8.2 | exon | 607259 | 607583 | 607259 | ID=MS.gene95083.t1.exon1;Parent=MS.gene95083.t1 |
| chr8.2 | CDS | 607259 | 607583 | 607259 | ID=cds.MS.gene95083.t1;Parent=MS.gene95083.t1 |
| chr8.2 | exon | 606492 | 606559 | 606492 | ID=MS.gene95083.t1.exon2;Parent=MS.gene95083.t1 |
| chr8.2 | CDS | 606492 | 606559 | 606492 | ID=cds.MS.gene95083.t1;Parent=MS.gene95083.t1 |
| Gene Sequence |
| Protein sequence |
